RchiOBHm_Chr1g0348461

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000400
Physical Location & Seq
Reverse (-)
41371864 .. 41375391
3528 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57450

Sequence Viewer

Length: 3528 bp
ATGGCAGACGTAGTTTTATCACCAGCTTTGCAAGTGATCTTTGACAGAGTAGCATCCCCAGTCCTACAAAAGCTTGCTGATATCTGGGACATGAAGGATAACCTCCGGAGCCTACAACATGCCTTGGTCATGGTTCAAGCAATTCTTGAAGATGCAGAGGAGCAACAACTGACCAACAAAGCCGTCCGGCTTTGGCTGTCGAAGCTCAAGAACGCAGCTCATGTTGCTGATGACCTTCTGGATTTCGTTACGGCCAAAGGAAAAGATAAGTGGACTTTAGCAATTTCAAAATCCATAGATGCTGACAAAGTCAGAAAGATGCTTTTGACATTAGAAATGACAGTAAACGAGGGTCTATTGAAGTTTAATTTTCGAGAGCGTGGTACAGTAGATAGGCAATCTGATAAAAGGGAGACTAGCTCTTTTGTTATGGAGTCAGAAATATATGGAAGAGCTGATGACAAAGAGAAGTTGGTGAAACTGTTGCTATCTTCTGAATGTCACCAGGATGGATATGCTATGTGTATTCCGATAATTGGTATTGGAGGAATTGGCAAGACGACTCTTGCTCAGTTGGCATATAATGATGAGCGGGTAATCCAACATTTTGATGTTAGGCTGTGGACTTTTGTCTCGGATTATTTCAATATCAAGAAGATCATGAAGTCAATTATCGAGTCTGTAACAAGGGAAGATTGCAAGTTCTCGGAGAGTGACTTACTTCAAGCTCGGCTTTGGCATTTGCTGCACAACAAAAGATATCTAATTGTGTTGGATGATGTTTGGACTGAAGATCAAGATGACTGGGACAAACTAAGACCTTTGTTTAGAGGAGGTGTTGATGGATGCAAAATCATTATCACTTCCCGCAATAAAAAAGTCCCATTCATGATGGGCTCCCCAACTTCGGCATATTATTTGAAGGGTTTGATGGAGGATGGCTGCTGGGCTTTGTTCAAGCAACGAGCTTTTGGACGAGGAGAAGAAGAGAAGTATGCCAACCTCTGCCTGATTGGAAAGAAGATTGTGAAGAAATGTGGTGGTGTACCATTGGCAGCCAAAAGTTTGGGAAGTGTTATGCGCTTTAAAAGAGAAGAACGGCAATGGTTGTTTATGCAAAATAGTGAACTGTGGGAAGTAGAAGGATGCAAACAAAGAATTTTACCTGCGCTTATGTTGAGTTATCTTCATCTGCCATCACATCTTCGACAATGCTTTGCATTCTGTTCCATATTTCCTAAAAATTATGAATTCAAGAAGCATAAATTAGTTCTCTTGTGGATGGCAGAGGGCTTAATTCTAGAAGAAAGTAGCAAGCGACCGGAAGACATTGGTGATGAATACTTTTCTGATTTGCTGTGGATGTCTTTCTTTCAAGAAGTAGAGTTACGTGATGGTGGCAGCACAGTTGGATACAAAATGAATGATGTCATTCATGACCTTGCACGATATGTTTCTGGAAATGAATCCGTTATGCTTGAACAAGGTCTTCCACCGAGTAATCCAGCACAAATTCGCCGCTCATCCATTATTTATAAATACAGGGAAGTTACAATTCCAGAAGCTCTCTTTGAAGCAGCACATTTACGAACTGTCTTATTGATTGGAGAATATGGACTTCTAAAAGACAGTAGTAAAATGTTATTAAGTTGTATATACTTGCGTGTGCTGGACTTGAACAACTGTGATGTTCATTTTTTGCCGTCATCAATAGGTGGGTTGATATGTTTGAGGTATCTCGACTTGTCTTATACACCTATCTCCAAGTTGCCGGCAACCACAAGGAATCTCTGTTCATTGCAGACTTTAAACCTCTCTGGATGTCATAATCTTAGAGTCTTGCCAGACTTCGAAGTAATGACCGGCCTAAGACATCTCAATATCGCTGGATGTGATAGGTTGTGGTATATACCTCCTGGTATTAAAATGTTACTTCAACTTCAGACACTGCCATTATATTTCGTTCAAAAGAGGGTCGGTGATCTTGGTGTACTAGAGAATTTGAATCTTTATGGGGAGTTGAATATTACACGCTTGGAGAATGTGAGAAGCGCAACTGGAGCTCTATCATCTGGACTGAAGATGAAAGAAAATCTTGAGTCGTTGGGATTATGTTGGGGAGATTTTGAAGATGTGAACGACAGGAAAAAATCATTGAAGAAACTAGCCAAGTCCCCAGCCAAAAGGTTTTTTTTAGAAGCAGGGGTACGGTGGCATGAACCTGATGCTGAAGAGGCGGTAGAAATTCTTGATGGCCTGAAACCACACAAGAATTTGAAAAAGCTAGTTATACATGGTTATCCAGGAATCAGATTTCCAGATTGGACTCTCCCAAATGTAATTGCAGTTGATTTGTCCAATTGTAAGAACTGTGAATATCTTCCAGCCCTTGGGAATCTTCGTCTACTCAAGACTCTTTCTCTGCACGGAATGGAGAGCGTGACACGCATTGGCTTTGAGTTCTATGGTGATAGTACAGACATTTGCTTTTCATCACTTGAAGAACTATCGCTCAGTGATTTTCCGAACTTGGAAACCTGGTCGAGTGCAAATAATGAAAATGCATTTCCGAGCTTGAGGAAGTTAACCATAAATAGGTGTCCCAAGTTGACACATATCCCATTTGGTTTGTCTCTTCAACATTTGGAGCTGCGGGATTGTAACCCATCTTCAATCTTCATTGGAGGTTTAACTTTGCTGACAGTGCTTGTAATAGAAAAACTTCCAGAGCTGCGCTCTCTACCACCAGGGCTTTCTGCATCAGCTAATCTGTCTTCTCTGGAGGTCTTATCTTGCCCCAAGCTTTGTTCCCTGCCTTTGGAGATCGGAAACCTCACTGCGCTGAAATCATTAACCATTCGTTGGTGTGATGAGTTGTCCTCTCTGCCACAGAGTTTGCAGAACCTCAAAGCTCTGGAGTCACTAGAGATTAGTGACTGTCATAGTATAATCTCGATGCCAGATGGTGCAATTGGAGGTTTGAGTTCCCTTCAAACTTTGTCCATTGAGAACTGCAGTAATCTGACGTCTTTGTCATCGAGTTTGGAACAACTCGCTTTCCTTGAGCGGTTCACCATTATGTACTGTCCAAACCTTGGTTCTTTCGCTGAGGGTGTGCAACACCTCTCAACCCTTCGAAGTTTGTCTATATTGAGTTGTCCTTGGTTTGATTCTTTGCCTGAAGGGTTGCAAATGGTCAAGACGTTGCAGTGTCTGGAAATTAGTAGCTGCCCGAATTTCACAGCTTTGCCAGAGTGGGTTGAGAAGCTTGCTTCACTCAGATCTTTGACCATTTTTGATTGTGCTAATTTGAAACTGTTGCCACAAAGTCTAAAGCTTCTCACTAAACTTCAGCACCTCTCTATTCAAGAATGTCCTGAGCTTGAGAGAAGATGTAGACAAGGAAGCGGTGAGGAGTGGCTTAAAATAGCTCATGTTCCACATAAGTACATTGGATCACCGGAGATCAGGCAGTCTGGTAGTGGAGCAAGCACATCCAACAGCTCTTCTTTTGATACATCTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1175

Amino Acids

132.41

Weight (kDa)

6.45

Isoelectric Point (pI)

52.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 85 6.5e-16 Rx N-terminal domain
NB-ARC PF00931 153 - 325 4.6e-36 NB-ARC domain
WHD_DRP PF23559 411 - 482 1.4e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 546 - 883 1.6e-22 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 663 - 811 4e-27 R13L1/DRL21 LRRs
LRR_14 PF23598 922 - 1121 4.3e-15 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1536
AasI GACNNNNNNGTC 1 cut(s) 3031
AatII GACGTC 1 cut(s) 3029
Acc36I ACCTGC 1 cut(s) 1174
AccB7I CCANNNNNTGG 3 cut(s) 2390, 2862, 3095
AccBSI CCGCTC 3 cut(s) 592, 1521, 3067
AccI GTMKAC 2 cut(s) 2404, 3397
AccIII TCCGGA 1 cut(s) 105
AciI CCGC 7 cut(s) 592, 868, 1519, 2237, 2653, 3067, 3408
AclWI GGATC 1 cut(s) 3463
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 7 cut(s) 1158, 1250, 1512, 1999, 2244, 2272, 3235
AcuI CTGAAG 6 cut(s) 810, 1925, 2099, 2250, 3201, 3335
AcyI GRCGYC 1 cut(s) 3026
AfaI GTAC 7 cut(s) 385, 1047, 1992, 2208, 2476, 3083, 3449
AfiI CCNNNNNNNGG 7 cut(s) 536, 907, 2390, 2595, 2818, 2862, 3095
AjnI CCWGG 5 cut(s) 504, 1915, 2302, 2537, 2746
AjuI GAANNNNNNNTTGG 2 cut(s) 3082, 3114
AloI GAACNNNNNNTCC 2 cut(s) 1777, 1809
Alw21I GWGCWC 1 cut(s) 2065
Alw26I GTCTC 3 cut(s) 407, 637, 2637
AlwI GGATC 1 cut(s) 3463
AlwNI CAGNNNCTG 2 cut(s) 1948, 3214
Aor13HI TCCGGA 1 cut(s) 105
AoxI GGCC 3 cut(s) 252, 1864, 2254
ApeKI GCWGC 9 cut(s) 215, 745, 942, 1055, 1401, 1577, 2650, 2731, 3228
ApoI RAATTY 7 cut(s) 1158, 1250, 1512, 1999, 2244, 2272, 3235
Asp700I GAANNNNTTC 2 cut(s) 2379, 2721
AspLEI GCGC 5 cut(s) 1083, 1171, 2054, 2736, 2842
AsuHPI GGTGA 9 cut(s) 12, 487, 494, 1346, 1991, 2480, 3064, 3422, 3450
AsuII TTCGAA 2 cut(s) 1851, 3136
BaeI ACNNNNGTAYC 1 cut(s) 3507
BanII GRGCYC 2 cut(s) 899, 2065
BarI GAAGNNNNNNTAC 6 cut(s) 1371, 1403, 2190, 2222, 2653, 2685
BbsI GAAGAC 3 cut(s) 1332, 1481, 2766
Bbv12I GWGCWC 1 cut(s) 2065
BbvCI CCTCAGC 1 cut(s) 3108
BbvI GCAGC 9 cut(s) 227, 732, 929, 1067, 1413, 1589, 2637, 2718, 3215
BceAI ACGGC 4 cut(s) 167, 267, 1115, 1687
BciT130I CCWGG 5 cut(s) 506, 1917, 2304, 2539, 2748
BciVI GTATCC 1 cut(s) 1406
BcoDI GTCTC 3 cut(s) 407, 637, 2637
BfaI CTAG 6 cut(s) 417, 1301, 1994, 2165, 2285, 2924
BfmI CTRYAG 1 cut(s) 3013
BfuAI ACCTGC 1 cut(s) 1174
BfuI GTATCC 1 cut(s) 1406
BglII AGATCT 1 cut(s) 3281
Bme1390I CCNGG 5 cut(s) 506, 1917, 2304, 2539, 2748
BmiI GGNNCC 2 cut(s) 110, 898
BmrFI CCNGG 5 cut(s) 506, 1917, 2304, 2539, 2748
BmrI ACTGGG 2 cut(s) 53, 814
BmsI GCATC 9 cut(s) 62, 142, 289, 309, 836, 1136, 2215, 2768, 2946
BmuI ACTGGG 2 cut(s) 53, 814
BoxI GACNNNNGTC 1 cut(s) 629
BpiI GAAGAC 3 cut(s) 1332, 1481, 2766
BplI GAGNNNNNCTC 6 cut(s) 404, 436, 2720, 2752, 2864, 2896
BpmI CTGGAG 3 cut(s) 2079, 2801, 2936
Bpu10I CCTNAGC 2 cut(s) 3108, 3378
Bpu14I TTCGAA 2 cut(s) 1851, 3136
BpuEI CTTGAG 6 cut(s) 191, 2117, 2393, 2596, 3083, 3404
BsaAI YACGTR 1 cut(s) 1391
BsaHI GRCGYC 1 cut(s) 3026
BsaJI CCNNGG 5 cut(s) 123, 2389, 2747, 3094, 3161
BsaWI WCCGGW 3 cut(s) 105, 1321, 3460
Bsc4I CCNNNNNNNGG 7 cut(s) 536, 907, 2390, 2595, 2818, 2862, 3095
Bse118I RCCGGY 2 cut(s) 1771, 1862
Bse1I ACTGG 3 cut(s) 59, 809, 2062
Bse3DI GCAATG 2 cut(s) 1109, 1796
BseAI TCCGGA 1 cut(s) 105
BseBI CCWGG 5 cut(s) 506, 1917, 2304, 2539, 2748
BseDI CCNNGG 5 cut(s) 123, 2389, 2747, 3094, 3161
BseLI CCNNNNNNNGG 7 cut(s) 536, 907, 2390, 2595, 2818, 2862, 3095
BseMI GCAATG 2 cut(s) 1109, 1796
BseMII CTCAG 5 cut(s) 584, 2527, 3099, 3292, 3369
BseNI ACTGG 3 cut(s) 59, 809, 2062
BseRI GAGGAG 4 cut(s) 173, 846, 993, 3428
BseXI GCAGC 9 cut(s) 227, 732, 929, 1067, 1413, 1589, 2637, 2718, 3215
BseYI CCCAGC 2 cut(s) 945, 2176
BsgI GTGCAG 2 cut(s) 731, 2408
Bsh1285I CGRYCG 1 cut(s) 1322
BshFI GGCC 3 cut(s) 254, 1866, 2256
BsiEI CGRYCG 1 cut(s) 1322
BsiHKAI GWGCWC 1 cut(s) 2065
BsiSI CCGG 6 cut(s) 106, 187, 1322, 1772, 1863, 3461
BslFI GGGAC 5 cut(s) 101, 821, 866, 2158, 2586
BslI CCNNNNNNNGG 7 cut(s) 536, 907, 2390, 2595, 2818, 2862, 3095
BsmAI GTCTC 3 cut(s) 407, 637, 2637
BsmFI GGGAC 5 cut(s) 101, 821, 866, 2158, 2586
BsmI GAATGC 1 cut(s) 1220
BsnI GGCC 3 cut(s) 254, 1866, 2256
Bsp119I TTCGAA 2 cut(s) 1851, 3136
Bsp1286I GDGCHC 2 cut(s) 899, 2065
Bsp13I TCCGGA 1 cut(s) 105
Bsp143I GATC 8 cut(s) 36, 657, 793, 1981, 2823, 3281, 3455, 3465
BspACI CCGC 7 cut(s) 592, 868, 1519, 2237, 2653, 3067, 3408
BspANI GGCC 3 cut(s) 254, 1866, 2256
BspCNI CTCAG 5 cut(s) 583, 2526, 3100, 3291, 3370
BspEI TCCGGA 1 cut(s) 105
BspHI TCATGA 3 cut(s) 660, 888, 1435
BspLI GGNNCC 2 cut(s) 110, 898
BspMAI CTGCAG 1 cut(s) 3017
BspMI ACCTGC 1 cut(s) 1174
BspPI GGATC 1 cut(s) 3463
BspQI GCTCTTC 2 cut(s) 445, 3511
BspT104I TTCGAA 2 cut(s) 1851, 3136
BsrBI CCGCTC 3 cut(s) 592, 1521, 3067
BsrDI GCAATG 2 cut(s) 1109, 1796
BsrFI RCCGGY 2 cut(s) 1771, 1862
BsrI ACTGG 3 cut(s) 59, 809, 2062
BssAI RCCGGY 2 cut(s) 1771, 1862
BssECI CCNNGG 5 cut(s) 123, 2389, 2747, 3094, 3161
BssMI GATC 8 cut(s) 36, 657, 793, 1981, 2823, 3281, 3455, 3465
BssNI GRCGYC 1 cut(s) 3026
BssT1I CCWWGG 4 cut(s) 123, 2389, 3094, 3161
Bst2UI CCWGG 5 cut(s) 506, 1917, 2304, 2539, 2748
Bst6I CTCTTC 5 cut(s) 445, 981, 2226, 2640, 3511
BstACI GRCGYC 1 cut(s) 3026
BstAPI GCANNNNNTGC 1 cut(s) 745
BstBAI YACGTR 1 cut(s) 1391
BstBI TTCGAA 2 cut(s) 1851, 3136
BstC8I GCNNGC 5 cut(s) 75, 1316, 1773, 3270, 3490
BstDEI CTNAG 8 cut(s) 570, 815, 1832, 1868, 2513, 3108, 3278, 3378
BstHHI GCGC 5 cut(s) 1083, 1171, 2054, 2736, 2842
BstKTI GATC 8 cut(s) 39, 660, 796, 1984, 2826, 3284, 3458, 3468
BstMAI GTCTC 3 cut(s) 407, 637, 2637
BstMBI GATC 8 cut(s) 36, 657, 793, 1981, 2823, 3281, 3455, 3465
BstMCI CGRYCG 1 cut(s) 1322
BstMWI GCNNNNNNNGC 8 cut(s) 202, 224, 575, 745, 2060, 2234, 2445, 2704
BstNI CCWGG 5 cut(s) 506, 1917, 2304, 2539, 2748
BstNSI RCATGY 1 cut(s) 122
BstPAI GACNNNNGTC 1 cut(s) 629
BstSCI CCNGG 5 cut(s) 504, 1915, 2302, 2537, 2746
BstSFI CTRYAG 1 cut(s) 3013
BstV1I GCAGC 9 cut(s) 227, 732, 929, 1067, 1413, 1589, 2637, 2718, 3215
BstV2I GAAGAC 3 cut(s) 1332, 1481, 2766
BstX2I RGATCY 1 cut(s) 3281
BstXI CCANNNNNNTGG 1 cut(s) 1066
BstYI RGATCY 1 cut(s) 3281
BsuI GTATCC 1 cut(s) 1406
BsuRI GGCC 3 cut(s) 254, 1866, 2256
BtsI GCAGTG 3 cut(s) 1946, 2835, 3215
BtsIMutI CAGTG 5 cut(s) 1946, 2521, 2709, 2835, 3215
BveI ACCTGC 1 cut(s) 1174
Cac8I GCNNGC 5 cut(s) 75, 1316, 1773, 3270, 3490
CaiI CAGNNNCTG 2 cut(s) 1948, 3214
CciI TCATGA 3 cut(s) 660, 888, 1435
CfoI GCGC 5 cut(s) 1083, 1171, 2054, 2736, 2842
Cfr10I RCCGGY 2 cut(s) 1771, 1862
CsiI ACCWGGT 1 cut(s) 2537
Csp6I GTAC 7 cut(s) 384, 1046, 1991, 2207, 2475, 3082, 3448
CspCI CAANNNNNGTGG 2 cut(s) 2877, 2912
CviQI GTAC 7 cut(s) 384, 1046, 1991, 2207, 2475, 3082, 3448
DdeI CTNAG 8 cut(s) 570, 815, 1832, 1868, 2513, 3108, 3278, 3378
DpnI GATC 8 cut(s) 38, 659, 795, 1983, 2825, 3283, 3457, 3467
DpnII GATC 8 cut(s) 36, 657, 793, 1981, 2823, 3281, 3455, 3465
DraI TTTAAA 2 cut(s) 1087, 1809
DrdI GACNNNNNNGTC 1 cut(s) 3031
DseDI GACNNNNNNGTC 1 cut(s) 3031
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 5 cut(s) 445, 981, 2226, 2640, 3511
EarI CTCTTC 5 cut(s) 445, 981, 2226, 2640, 3511
Ecl136II GAGCTC 1 cut(s) 2063
Eco130I CCWWGG 4 cut(s) 123, 2389, 3094, 3161
Eco24I GRGCYC 2 cut(s) 899, 2065
Eco32I GATATC 2 cut(s) 82, 761
Eco53kI GAGCTC 1 cut(s) 2063
Eco57I CTGAAG 6 cut(s) 810, 1925, 2099, 2250, 3201, 3335
EcoICRI GAGCTC 1 cut(s) 2063
EcoRI GAATTC 1 cut(s) 1250
EcoRII CCWGG 5 cut(s) 504, 1915, 2302, 2537, 2746
EcoRV GATATC 2 cut(s) 82, 761
EcoT14I CCWWGG 4 cut(s) 123, 2389, 3094, 3161
EcoT22I ATGCAT 1 cut(s) 2566
EcoT38I GRGCYC 2 cut(s) 899, 2065
ErhI CCWWGG 4 cut(s) 123, 2389, 3094, 3161
FalI AAGNNNNNCTT 6 cut(s) 129, 161, 717, 749, 2079, 2111
FaqI GGGAC 5 cut(s) 101, 821, 866, 2158, 2586
FauI CCCGC 3 cut(s) 585, 875, 2646
FblI GTMKAC 2 cut(s) 2404, 3397
FriOI GRGCYC 2 cut(s) 899, 2065
FspBI CTAG 6 cut(s) 417, 1301, 1994, 2165, 2285, 2924
GlaI GCGC 5 cut(s) 1082, 1170, 2053, 2735, 2841
GsaI CCCAGC 2 cut(s) 949, 2180
GsuI CTGGAG 3 cut(s) 2079, 2801, 2936
HaeIII GGCC 3 cut(s) 254, 1866, 2256
HapII CCGG 6 cut(s) 106, 187, 1322, 1772, 1863, 3461
HhaI GCGC 5 cut(s) 1083, 1171, 2054, 2736, 2842
Hin1I GRCGYC 1 cut(s) 3026
Hin6I GCGC 5 cut(s) 1081, 1169, 2052, 2734, 2840
HinP1I GCGC 5 cut(s) 1081, 1169, 2052, 2734, 2840
HincII GTYRAC 2 cut(s) 2586, 2610
HindII GTYRAC 2 cut(s) 2586, 2610
HindIII AAGCTT 4 cut(s) 71, 2801, 3266, 3335
HpaI GTTAAC 1 cut(s) 2586
HpaII CCGG 6 cut(s) 106, 187, 1322, 1772, 1863, 3461
HphI GGTGA 9 cut(s) 12, 487, 494, 1346, 1991, 2480, 3064, 3422, 3450
HpyAV CCTTC 7 cut(s) 88, 245, 916, 1136, 2999, 3143, 3176
HpyCH4IV ACGT 4 cut(s) 9, 1390, 3026, 3203
HpyF10VI GCNNNNNNNGC 8 cut(s) 202, 224, 575, 745, 2060, 2234, 2445, 2704
HpyF3I CTNAG 8 cut(s) 570, 815, 1832, 1868, 2513, 3108, 3278, 3378
HpySE526I ACGT 4 cut(s) 9, 1390, 3026, 3203
Hsp92I GRCGYC 1 cut(s) 3026
HspAI GCGC 5 cut(s) 1081, 1169, 2052, 2734, 2840
Kpn2I TCCGGA 1 cut(s) 105
KroI GCCGGC 1 cut(s) 1771
KroNI GCCGGC 1 cut(s) 1773
KspAI GTTAAC 1 cut(s) 2586
Kzo9I GATC 8 cut(s) 36, 657, 793, 1981, 2823, 3281, 3455, 3465
LguI GCTCTTC 2 cut(s) 445, 3511
LmnI GCTCC 6 cut(s) 108, 160, 902, 2060, 2647, 3485
Lsp1109I GCAGC 9 cut(s) 227, 732, 929, 1067, 1413, 1589, 2637, 2718, 3215
LweI GCATC 9 cut(s) 62, 142, 289, 309, 836, 1136, 2215, 2768, 2946
MabI ACCWGGT 1 cut(s) 2537
MaeI CTAG 6 cut(s) 417, 1301, 1994, 2165, 2285, 2924
MaeII ACGT 4 cut(s) 9, 1390, 3026, 3203
MalI GATC 8 cut(s) 38, 659, 795, 1983, 2825, 3283, 3457, 3467
MbiI CCGCTC 3 cut(s) 592, 1521, 3067
MboI GATC 8 cut(s) 36, 657, 793, 1981, 2823, 3281, 3455, 3465
MfeI CAATTG 2 cut(s) 2359, 2970
MflI RGATCY 1 cut(s) 3281
MhlI GDGCHC 2 cut(s) 899, 2065
MlyI GAGTC 8 cut(s) 443, 556, 686, 1845, 2108, 2320, 2407, 2927
MmeI TCCRAC 4 cut(s) 625, 753, 1390, 3522
Mph1103I ATGCAT 1 cut(s) 2566
MroI TCCGGA 1 cut(s) 105
MroNI GCCGGC 1 cut(s) 1771
MroXI GAANNNNTTC 2 cut(s) 2379, 2721
MslI CAYNNNNRTG 3 cut(s) 507, 609, 3077
MspI CCGG 6 cut(s) 106, 187, 1322, 1772, 1863, 3461
MspR9I CCNGG 5 cut(s) 506, 1917, 2304, 2539, 2748
MunI CAATTG 2 cut(s) 2359, 2970
Mva1269I GAATGC 1 cut(s) 1220
MvaI CCWGG 5 cut(s) 506, 1917, 2304, 2539, 2748
MwoI GCNNNNNNNGC 8 cut(s) 202, 224, 575, 745, 2060, 2234, 2445, 2704
NaeI GCCGGC 1 cut(s) 1773
NdeII GATC 8 cut(s) 36, 657, 793, 1981, 2823, 3281, 3455, 3465
NgoMIV GCCGGC 1 cut(s) 1771
NlaIV GGNNCC 2 cut(s) 110, 898
NmeAIII GCCGAG 1 cut(s) 709
NmuCI GTSAC 5 cut(s) 500, 713, 2440, 2919, 2933
NsiI ATGCAT 1 cut(s) 2566
NspI RCATGY 1 cut(s) 122
NspV TTCGAA 2 cut(s) 1851, 3136
PagI TCATGA 3 cut(s) 660, 888, 1435
PciSI GCTCTTC 2 cut(s) 445, 3511
PctI GAATGC 1 cut(s) 1220
PdiI GCCGGC 1 cut(s) 1773
PdmI GAANNNNTTC 2 cut(s) 2379, 2721
PfeI GAWTC 6 cut(s) 1466, 1786, 2005, 2307, 2395, 3170
PflFI GACNNNGTC 1 cut(s) 308
PflMI CCANNNNNTGG 3 cut(s) 2390, 2862, 3095
PfoI TCCNGGA 1 cut(s) 2302
PleI GAGTC 8 cut(s) 442, 556, 685, 1844, 2107, 2320, 2407, 2926
PpsI GAGTC 8 cut(s) 442, 556, 685, 1844, 2107, 2320, 2407, 2926
Ppu21I YACGTR 1 cut(s) 1391
PshAI GACNNNNGTC 1 cut(s) 629
PsiI TTATAA 1 cut(s) 1536
Psp124BI GAGCTC 1 cut(s) 2065
Psp6I CCWGG 5 cut(s) 504, 1915, 2302, 2537, 2746
PspFI CCCAGC 2 cut(s) 945, 2176
PspGI CCWGG 5 cut(s) 504, 1915, 2302, 2537, 2746
PspN4I GGNNCC 2 cut(s) 110, 898
PstI CTGCAG 1 cut(s) 3017
PstNI CAGNNNCTG 2 cut(s) 1948, 3214
PsuI RGATCY 1 cut(s) 3281
PsyI GACNNNGTC 1 cut(s) 308
RsaI GTAC 7 cut(s) 385, 1047, 1992, 2208, 2476, 3083, 3449
RsaNI GTAC 7 cut(s) 384, 1046, 1991, 2207, 2475, 3082, 3448
RseI CAYNNNNRTG 3 cut(s) 507, 609, 3077
SacI GAGCTC 1 cut(s) 2065
SapI GCTCTTC 2 cut(s) 445, 3511
Sau3AI GATC 8 cut(s) 36, 657, 793, 1981, 2823, 3281, 3455, 3465
SchI GAGTC 8 cut(s) 443, 556, 686, 1845, 2108, 2320, 2407, 2927
ScrFI CCNGG 5 cut(s) 506, 1917, 2304, 2539, 2748
SduI GDGCHC 2 cut(s) 899, 2065
SexAI ACCWGGT 1 cut(s) 2537
SfaNI GCATC 9 cut(s) 62, 142, 289, 309, 836, 1136, 2215, 2768, 2946
SfcI CTRYAG 1 cut(s) 3013
SfuI TTCGAA 2 cut(s) 1851, 3136
SmiMI CAYNNNNRTG 3 cut(s) 507, 609, 3077
SmlI CTYRAG 6 cut(s) 206, 2096, 2408, 2575, 3062, 3383
SmoI CTYRAG 6 cut(s) 206, 2096, 2408, 2575, 3062, 3383
SsiI CCGC 7 cut(s) 592, 868, 1519, 2237, 2653, 3067, 3408
SspI AATATT 1 cut(s) 2026
SspMI CTAG 6 cut(s) 417, 1301, 1994, 2165, 2285, 2924
SstI GAGCTC 1 cut(s) 2065
StyD4I CCNGG 5 cut(s) 504, 1915, 2302, 2537, 2746
StyI CCWWGG 4 cut(s) 123, 2389, 3094, 3161
TaiI ACGT 4 cut(s) 12, 1393, 3029, 3206
TatI WGTACW 4 cut(s) 1990, 2474, 3081, 3447
TauI GCSGC 1 cut(s) 1521
TfiI GAWTC 6 cut(s) 1466, 1786, 2005, 2307, 2395, 3170
TscAI CASTG 5 cut(s) 1953, 2521, 2709, 2842, 3215
TseFI GTSAC 5 cut(s) 500, 713, 2440, 2919, 2933
TseI GCWGC 9 cut(s) 215, 745, 942, 1055, 1401, 1577, 2650, 2731, 3228
Tsp45I GTSAC 5 cut(s) 500, 713, 2440, 2919, 2933
TspGWI ACGGA 2 cut(s) 1459, 2442
TspRI CASTG 5 cut(s) 1953, 2521, 2709, 2842, 3215
Tth111I GACNNNGTC 1 cut(s) 308
Van91I CCANNNNNTGG 3 cut(s) 2390, 2862, 3095
XapI RAATTY 7 cut(s) 1158, 1250, 1512, 1999, 2244, 2272, 3235
XbaI TCTAGA 1 cut(s) 1300
XceI RCATGY 1 cut(s) 122
XmiI GTMKAC 2 cut(s) 2404, 3397
XmnI GAANNNNTTC 2 cut(s) 2379, 2721
XspI CTAG 6 cut(s) 417, 1301, 1994, 2165, 2285, 2924
ZraI GACGTC 1 cut(s) 3027
Zsp2I ATGCAT 1 cut(s) 2566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.