RchiOBHm_Chr7g0232961

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000400
Physical Location & Seq
Reverse (-)
57174178 .. 57174936
759 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20880

Sequence Viewer

Length: 759 bp
ATGTCATCAGTTGTATGCAGTTCTAGCTCTGCTTTGATCCCTGAAGCCTTGTGTGAAGCAAAGAGATTGCGAACCCTCAATTTCCTGTCGCCAAGAGAGGATTATATGGAAGCCATTCCAACCATACTAGCAACTTTTAAACATCTCAGAATGCTGAATTTCAGTGGATCTGGAATTAAGAGTCTCCACCAGGAGATTGGTGGGCTAATATCCTTGCGGTATCTTGATCTGTCAAATACTACCCTAGAGACGATGCCAGCGACTATCTGTGATCGCTGCCATTTGCAGACCCTGAATCTCTCAAGTTGTCGTGAGCTCAAGGAGTTACCAAGTGGTACTACCAAGTTAATAAATCTGAGACATCTTAACATAGATGATTGTCCAAGACTTGCTGGCATGCCCCCATCGATGGGAATTTTACAACAACTTCAAACTTTGCCAGTATATATCATCGGCCGCAATTTTGAAACTTCTATTTTTCAGATTATCTCAATGAATCTACGAGGGAAGTTAAAAATCAAATGTCTGGAGGAGGCTAAAATTCCATTTGGAAACAACATGATTAAAAGATGGATGCTAACGAAAGAGTTTCAGTCATTGGAACTGTTGTGGCAAAATGATGGGGGCAAGCTAGATCATAATAGATCTAGGCAAGCTGGCAGGCAAGTTGATGATAGAACTGAATTTTGTCTGGTAGATTCTTTGACTGTATCGCCCTTTATAAGAATGTTGTCAATAAATGGTTATTCAGGAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

252

Amino Acids

28.42

Weight (kDa)

8.8

Isoelectric Point (pI)

57.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 23 - 203 2.1e-19 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 722
AciI CCGC 2 cut(s) 217, 457
AclWI GGATC 2 cut(s) 31, 175
AcoI YGGCCR 1 cut(s) 454
AcsI RAATTY 4 cut(s) 157, 414, 540, 683
AcuI CTGAAG 1 cut(s) 63
AfaI GTAC 1 cut(s) 337
AfiI CCNNNNNNNGG 2 cut(s) 409, 410
AgsI TTSAA 2 cut(s) 431, 467
AjnI CCWGG 1 cut(s) 189
AluBI AGCT 4 cut(s) 27, 316, 631, 656
AluI AGCT 4 cut(s) 27, 316, 631, 656
Alw21I GWGCWC 1 cut(s) 318
Alw26I GTCTC 3 cut(s) 188, 242, 352
AlwI GGATC 2 cut(s) 31, 175
AlwNI CAGNNNCTG 1 cut(s) 292
AoxI GGCC 1 cut(s) 454
ApeKI GCWGC 1 cut(s) 276
ApoI RAATTY 4 cut(s) 157, 414, 540, 683
Asp700I GAANNNNTTC 1 cut(s) 114
BanII GRGCYC 1 cut(s) 318
Bbv12I GWGCWC 1 cut(s) 318
BbvI GCAGC 1 cut(s) 263
BccI CCATC 4 cut(s) 403, 412, 564, 614
BciT130I CCWGG 1 cut(s) 191
BcoDI GTCTC 3 cut(s) 188, 242, 352
BfaI CTAG 6 cut(s) 24, 128, 245, 632, 648, 757
BglII AGATCT 1 cut(s) 644
BisI GCNGC 2 cut(s) 277, 457
BlsI GCNGC 2 cut(s) 278, 458
Bme1390I CCNGG 1 cut(s) 191
BmiI GGNNCC 1 cut(s) 754
BmrFI CCNGG 1 cut(s) 191
BmsI GCATC 2 cut(s) 243, 564
BpmI CTGGAG 1 cut(s) 548
BpuEI CTTGAG 2 cut(s) 286, 302
Bsa29I ATCGAT 1 cut(s) 407
Bsc4I CCNNNNNNNGG 2 cut(s) 409, 410
Bse1I ACTGG 1 cut(s) 440
BseBI CCWGG 1 cut(s) 191
BseCI ATCGAT 1 cut(s) 407
BseGI GGATG 1 cut(s) 579
BseLI CCNNNNNNNGG 2 cut(s) 409, 410
BseMII CTCAG 2 cut(s) 160, 347
BseNI ACTGG 1 cut(s) 440
BseRI GAGGAG 1 cut(s) 545
BseX3I CGGCCG 1 cut(s) 454
BseXI GCAGC 1 cut(s) 263
Bsh1285I CGRYCG 1 cut(s) 457
BshFI GGCC 1 cut(s) 456
BshVI ATCGAT 1 cut(s) 407
BsiEI CGRYCG 1 cut(s) 457
BsiHKAI GWGCWC 1 cut(s) 318
BslI CCNNNNNNNGG 2 cut(s) 409, 410
BsmAI GTCTC 3 cut(s) 188, 242, 352
BsmBI CGTCTC 1 cut(s) 242
BsmI GAATGC 1 cut(s) 156
BsnI GGCC 1 cut(s) 456
Bsp1286I GDGCHC 1 cut(s) 318
Bsp143I GATC 6 cut(s) 36, 167, 226, 271, 634, 644
BspACI CCGC 2 cut(s) 217, 457
BspANI GGCC 1 cut(s) 456
BspCNI CTCAG 2 cut(s) 159, 348
BspDI ATCGAT 1 cut(s) 407
BspLI GGNNCC 1 cut(s) 754
BspPI GGATC 2 cut(s) 31, 175
BsrI ACTGG 1 cut(s) 440
BssMI GATC 6 cut(s) 36, 167, 226, 271, 634, 644
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 2 cut(s) 606, 709
BstC8I GCNNGC 7 cut(s) 258, 394, 398, 629, 654, 658, 662
BstDEI CTNAG 2 cut(s) 146, 356
BstF5I GGATG 1 cut(s) 579
BstKTI GATC 6 cut(s) 39, 170, 229, 274, 637, 647
BstMAI GTCTC 3 cut(s) 188, 242, 352
BstMBI GATC 6 cut(s) 36, 167, 226, 271, 634, 644
BstMCI CGRYCG 1 cut(s) 457
BstMWI GCNNNNNNNGC 1 cut(s) 24
BstNI CCWGG 1 cut(s) 191
BstNSI RCATGY 1 cut(s) 400
BstSCI CCNGG 1 cut(s) 189
BstV1I GCAGC 1 cut(s) 263
BstX2I RGATCY 2 cut(s) 167, 644
BstXI CCANNNNNNTGG 1 cut(s) 197
BstYI RGATCY 2 cut(s) 167, 644
BstZI CGGCCG 1 cut(s) 454
Bsu15I ATCGAT 1 cut(s) 407
BsuRI GGCC 1 cut(s) 456
BsuTUI ATCGAT 1 cut(s) 407
BtsCI GGATG 1 cut(s) 579
BtsIMutI CAGTG 1 cut(s) 169
Cac8I GCNNGC 7 cut(s) 258, 394, 398, 629, 654, 658, 662
CaiI CAGNNNCTG 1 cut(s) 292
ClaI ATCGAT 1 cut(s) 407
Csp6I GTAC 1 cut(s) 336
CviAII CATG 2 cut(s) 397, 559
CviJI RGCY 9 cut(s) 27, 47, 113, 205, 316, 456, 536, 631, 656
CviKI_1 RGCY 9 cut(s) 27, 47, 113, 205, 316, 456, 536, 631, 656
CviQI GTAC 1 cut(s) 336
DdeI CTNAG 2 cut(s) 146, 356
DpnI GATC 6 cut(s) 38, 169, 228, 273, 636, 646
DpnII GATC 6 cut(s) 36, 167, 226, 271, 634, 644
DraI TTTAAA 1 cut(s) 139
EaeI YGGCCR 1 cut(s) 454
EagI CGGCCG 1 cut(s) 454
Ecl136II GAGCTC 1 cut(s) 316
EclXI CGGCCG 1 cut(s) 454
Eco24I GRGCYC 1 cut(s) 318
Eco52I CGGCCG 1 cut(s) 454
Eco53kI GAGCTC 1 cut(s) 316
Eco57I CTGAAG 1 cut(s) 63
EcoICRI GAGCTC 1 cut(s) 316
EcoRII CCWGG 1 cut(s) 189
EcoT38I GRGCYC 1 cut(s) 318
Esp3I CGTCTC 1 cut(s) 242
FaeI CATG 2 cut(s) 400, 562
FatI CATG 2 cut(s) 396, 558
Fnu4HI GCNGC 2 cut(s) 277, 457
FokI GGATG 1 cut(s) 586
FriOI GRGCYC 1 cut(s) 318
Fsp4HI GCNGC 2 cut(s) 277, 457
FspBI CTAG 6 cut(s) 24, 128, 245, 632, 648, 757
GluI GCNGC 2 cut(s) 277, 457
GsuI CTGGAG 1 cut(s) 548
HaeIII GGCC 1 cut(s) 456
Hin1II CATG 2 cut(s) 400, 562
HinfI GANTC 4 cut(s) 181, 295, 496, 698
Hpy188I TCNGA 3 cut(s) 149, 357, 483
Hpy188III TCNNGA 5 cut(s) 171, 224, 311, 527, 750
HpyCH4III ACNGT 2 cut(s) 606, 709
HpyCH4V TGCA 2 cut(s) 18, 286
HpyF10VI GCNNNNNNNGC 1 cut(s) 24
HpyF3I CTNAG 2 cut(s) 146, 356
Hsp92II CATG 2 cut(s) 400, 562
Kzo9I GATC 6 cut(s) 36, 167, 226, 271, 634, 644
Lsp1109I GCAGC 1 cut(s) 263
LweI GCATC 2 cut(s) 243, 564
MaeI CTAG 6 cut(s) 24, 128, 245, 632, 648, 757
MaeIII GTNAC 1 cut(s) 324
MalI GATC 6 cut(s) 38, 169, 228, 273, 636, 646
MboI GATC 6 cut(s) 36, 167, 226, 271, 634, 644
MflI RGATCY 2 cut(s) 167, 644
MhlI GDGCHC 1 cut(s) 318
MluCI AATT 7 cut(s) 79, 157, 174, 414, 460, 540, 683
MlyI GAGTC 1 cut(s) 190
MmeI TCCRAC 1 cut(s) 143
MnlI CCTC 5 cut(s) 86, 91, 497, 523, 526
MroXI GAANNNNTTC 1 cut(s) 114
MseI TTAA 6 cut(s) 138, 177, 347, 366, 512, 564
MspR9I CCNGG 1 cut(s) 191
Mva1269I GAATGC 1 cut(s) 156
MvaI CCWGG 1 cut(s) 191
MwoI GCNNNNNNNGC 1 cut(s) 24
NdeII GATC 6 cut(s) 36, 167, 226, 271, 634, 644
NlaIII CATG 2 cut(s) 400, 562
NlaIV GGNNCC 1 cut(s) 754
NspI RCATGY 1 cut(s) 400
PaeI GCATGC 1 cut(s) 400
PcsI WCGNNNNNNNCGW 1 cut(s) 257
PctI GAATGC 1 cut(s) 156
PdmI GAANNNNTTC 1 cut(s) 114
PfeI GAWTC 3 cut(s) 295, 496, 698
PkrI GCNGC 2 cut(s) 278, 458
PleI GAGTC 1 cut(s) 189
PpsI GAGTC 1 cut(s) 189
PsiI TTATAA 1 cut(s) 722
Psp124BI GAGCTC 1 cut(s) 318
Psp6I CCWGG 1 cut(s) 189
PspGI CCWGG 1 cut(s) 189
PspN4I GGNNCC 1 cut(s) 754
PstNI CAGNNNCTG 1 cut(s) 292
PsuI RGATCY 2 cut(s) 167, 644
RsaI GTAC 1 cut(s) 337
RsaNI GTAC 1 cut(s) 336
SacI GAGCTC 1 cut(s) 318
SaqAI TTAA 6 cut(s) 138, 177, 347, 366, 512, 564
SatI GCNGC 2 cut(s) 277, 457
Sau3AI GATC 6 cut(s) 36, 167, 226, 271, 634, 644
SchI GAGTC 1 cut(s) 190
ScrFI CCNGG 1 cut(s) 191
SduI GDGCHC 1 cut(s) 318
SetI ASST 5 cut(s) 29, 318, 633, 658, 758
SfaNI GCATC 2 cut(s) 243, 564
SmlI CTYRAG 2 cut(s) 301, 317
SmoI CTYRAG 2 cut(s) 301, 317
SphI GCATGC 1 cut(s) 400
Sse9I AATT 7 cut(s) 79, 157, 174, 414, 460, 540, 683
SsiI CCGC 2 cut(s) 217, 457
SspMI CTAG 6 cut(s) 24, 128, 245, 632, 648, 757
SstI GAGCTC 1 cut(s) 318
StyD4I CCNGG 1 cut(s) 189
TaaI ACNGT 2 cut(s) 606, 709
TaqI TCGA 1 cut(s) 407
TasI AATT 7 cut(s) 79, 157, 174, 414, 460, 540, 683
TauI GCSGC 1 cut(s) 459
TfiI GAWTC 3 cut(s) 295, 496, 698
Tru1I TTAA 6 cut(s) 138, 177, 347, 366, 512, 564
Tru9I TTAA 6 cut(s) 138, 177, 347, 366, 512, 564
TscAI CASTG 1 cut(s) 169
TseI GCWGC 1 cut(s) 276
TspDTI ATGAA 1 cut(s) 509
TspRI CASTG 1 cut(s) 169
XapI RAATTY 4 cut(s) 157, 414, 540, 683
XceI RCATGY 1 cut(s) 400
XcmI CCANNNNNNNNNTGG 2 cut(s) 194, 197
XmnI GAANNNNTTC 1 cut(s) 114
XspI CTAG 6 cut(s) 24, 128, 245, 632, 648, 757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.