Rroxscaffold_4G00306930

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000400
Physical Location & Seq
Forward (+)
27920840 .. 27924391
3552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00306930.1

Sequence Viewer

Length: 3552 bp
ATGGCAGACGTAGTTTTATCACCAGCTTTGCAAGTGATCTTTGACAGAGTAGCATCCCCAGTCCTACAAAAGCTTGCTGATATCTGGGACATGAAGGACAACCTCCAGAGCCTACAACATGCCTTGGTCATGGTTCAAGCTATTCTTGAAGATGCAGAGGAGCAACAACTGACCAACAAAGCCGTCCGGCTTTGGCTGTTGAAGCTCAAGAACGCAGCTCATGATGCTGATGACCTTCTGGATTTCGTTACGGCCAAAGGAAAAGATAAGTGGACTATAGCAATTTCAAAATCCATAGATGCTGACAAAGTCAGAAAGATGCTTTTGACATTAGAAATGACAGTAAACGAGGGTCTATTGAAGTTTAATTTTCGAGAGCCTGGTACAGTAGATAGGCAATCTGACAAAAGGGAGACTAGCTCTTTTGTTATGGAGTCAGAAATATATGGAAGAGCTGATGACAAAGAGAAGTTAGTGAAACTGTTACTATCTTCTGAGTGTCACCAGGATGGTTATGCTATGTGTATTTCGATAATTGGTATTGGAGGAATTGGCAAGACGACTCTTGCTCAGTTGGCATATAATGATGAGCGGGTAATCCAACATTTTGATGTTAGGCTGTGGACTTTTGTCTCGCATGATTTCAATATCAAGAAGATCATGAAGTCAATTATTGAGTCTGTAACAAGGGAAGATTGCAAGTTCTCGGAGAGTGACTTACTTCAATCTCGACTTTGGCATTTGCTGCACAACAAAAGATATCTAATTGTGTTGGATGATGTTTGGACTGAAGATCAAGATGACTGGGACAAACTAAGACCTTTGTTTAGACGAGGTGTTGATGGATGCAAAATCATTATCACTTCCCGCAATAAAAAAATCCCATTCATGATGGACTCCCTAACTTCGGCATACTATTTAAAGGGTTTGATGGAGGATGACTGCTGGGCTTTGTTCAAGCAACGGGCTTTTGGACGAGGAGAAGAAGAGAAGTATCCCGACCTCTGCCTGATTGGAAAGCAGATTGCAAAGAAATGTGGGGGCGTACCATTAGCAGCCAAAAGTTTGGGAAGTGTTATGCGCTTTAAAAGAGAAGAACGGCAATGGTTGTTTATGCAAAATAGTGAACTTTGGGAAGTAGATGGATGCAAACAGAGAATTTTACCTGCGCTTATGTTAAGTTATCTTCATCTGCCAGCACATCTTCGACAATGCTTTGCATTCTGCTCCATATTTCCTAAAAATTATGAATTCAAGAAGCATAAATTAGTTCTCTTGTGGATGGCAGAGGGCTTAATTCTGGAAGAAGGTAGCAAGCGACCAGAAGACATTGGTGACGAATACTTTTCTGATTTGCTGTGGATGTCTTTCTTTCAAGAAGTAGAGTTACGTGATGGTGGTAGCACAATTGGATACAAAATGAATGATGTCATTCATGATCTTGCACGATATGTTTCTGGAAATGAATCCGTTATGCTTGAACAAGGTCTTCCACCAAGTAATCCAGCACAAATTCGCCGCTCATCCATTATTTATAAATACAGGGAAGTTACAATTCCAGAAGCTCTCTTTGAAGCAGAACATTTACGAACTGTCTTAATGATTGGAGAATATGGACTTCTAAAAGACAGTAGTAAAATGTTATTAAGTTGTATATACTTGCGTGTGCTGGACTTGAACAACTGTGAAGTTCATTTTTTGCCGGCATCAATAGGTGGGTTGATATGTTTGAGGTATCTCGACTTGTCTTATACGCCAATCTCCAAGTTGCCGGCAACCACAAGGAATCTCTGTTCATTACAGACTTTAAACCTCTCTGGATGTCATAATCTTAGAGTCTTGCGAGACTTCGAAGCAATGACCAGCCTAAGACATCTCAATATAACTGGATGTGATAGCTTGTGGTATATATCTCCTTGTATTAAAATGTTACTTCAACTTCAGACACTGCCATTATATTTCGTTCGCAAGAATCCCGGTAATCTTGGTGCACTAGAGAATTTGAATCTTTATGGGGAGTTGAATATTACACACTTGGAGAATATAAGAAATGCAACTGGAGCTCAATCATCTGGACTGAAGATGAAAGAAAATATTGAGTCGTTGGGATTATACTGGGGAGATTTTGGAGATGTGCACGATAGGAATAAATCATTGAAGAAACCAGCCAAGTCCTCAGCCAAATGGTACTGTTCAGAAGTAGGGATGCGGTGGCATGAACCTGATGCTGAAGAGGCGGTAGAAATTCTTGATGGCCTGAAACCACACAAGAATTTGAAAAAGCTTGTTATACATGGTTATCCAGGAACCAGATTTCCAGATTGGAAACTGCCAAATCTAATTGCAGTTGATTTGTCCAATTGTAAGAACTGTGAATTTCTTCCAGCCCTTGGGAATATTCTTCTACTCAAGACTCTTTCTCTGCATGGAATGGAGAGCGTGACGCGCATTGGCTATGAGTTCTATGGTGATAGTACAGACATATGCTTTTTATCACTTGAAGAACTATCGCTCAGTGATTTTCCGAAATTGGAAACCTGGTCGAGTGCAAATAATGAAAATGCGTTTCCGAGCTTGAGGAAGTTAACCATACGCAGGTGTCCCAAGTTGACACAGATACCATTTTGTTTGTCTCTTCAACATTTGGAGCTGCGGGATTGTAATCCATCTTCAATCTTCATTGGAGGTTTAACTTTGCTGACAGTGCTTGTAATAGAAAAACTTCCAGAGCTGCGCTCTCTACCACAAGGGCTTTCTGCATCAGCTAGTCTGTCTTCTCTGGAGGTCTTATCTTGCCCCAAGCTTTGTTCACTGCCTTCGGACATGGGAAACCTCACTGCACTGAAATCATTGACCATTCGTTGGTGTGATGAGTTGTCCTATCTGCCACAGAGTTTGAAAAACCTCAAAGCTCTGGAGTCACTAGAGATTAGTGACTGTCATAGTATAATCTCGATGCCAGATGGTGCAATTGGAGGTTTGAGTTCCCTTCAAACTTTGTCCATTGAGAACTGCAGTAGTCTGACTTCTTTGTCTTCGAGTCTGGAACAACTTGCTTTCCTTGAGCGGTTCACCATTATGTACTGTCCAAACCTTGGTTCTTTCCCAGAGGGTGTGCAACACCTCTCAACCCTTCGAACTTTGTCTATCTTGAGCTGTCCTTGGTTTGATTCTTTGCCAGAAGGGTTGCAAATGGTCAAGACGTTGCAGTGTCTGGAAATTAGTAGCTGCCCGAATTTCACAGCTTTGCCAGAGTGGGTTGAGAAGCTTGCTTCACTCAGGTCTTTGACCATTTTTGATTGTCCTAATTTGAAACTGTTGCCACAAAGTCTAGAACTTCTCACTAAACTCCAGCACCTCTCTATTCAAGAATGTCCCGAGCTTGAGGAAAGATGTAGACAAGGCAGTGGTGAGGATTGGCTGAAAATAGCTCATGTTCCACATAAGTACATTGGATCACCGGAGATGAGGCGGTCCAGTAGTGAAGCAAGTACATCGGGCAGCTCTTCTGTTGAAACATCTGTACAGCAAGCAAATGCTTCATCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1183

Amino Acids

133.66

Weight (kDa)

6.0

Isoelectric Point (pI)

54.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 85 9.3e-17 Rx N-terminal domain
NB-ARC PF00931 153 - 325 2.7e-37 NB-ARC domain
WHD_DRP PF23559 411 - 482 1.2e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 546 - 882 5e-18 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 663 - 811 3.4e-26 R13L1/DRL21 LRRs
LRR_14 PF23598 879 - 1018 1.3e-07 Leucine-rich repeat region
LRR_14 PF23598 1031 - 1125 8.3e-08 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 1061 - 1125 1e-05 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1536
AarI CACCTGC 1 cut(s) 2586
AasI GACNNNNNNGTC 1 cut(s) 3031
Acc36I ACCTGC 2 cut(s) 1174, 2586
AccB7I CCANNNNNTGG 3 cut(s) 2390, 2862, 3095
AccBSI CCGCTC 3 cut(s) 592, 1521, 3067
AccI GTMKAC 1 cut(s) 3397
AccII CGCG 1 cut(s) 2446
AciI CCGC 8 cut(s) 592, 868, 1519, 2209, 2237, 2653, 3067, 3472
AclWI GGATC 1 cut(s) 3463
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 8 cut(s) 1158, 1250, 1512, 1999, 2244, 2272, 2375, 3235
AcuI CTGAAG 4 cut(s) 810, 1925, 2099, 2250
AfaI GTAC 8 cut(s) 385, 1047, 2189, 2476, 3083, 3449, 3493, 3525
AfiI CCNNNNNNNGG 5 cut(s) 907, 2390, 2595, 2862, 3095
AjnI CCWGG 4 cut(s) 379, 504, 2302, 2537
AjuI GAANNNNNNNTTGG 2 cut(s) 3082, 3114
AloI GAACNNNNNNTCC 4 cut(s) 1777, 1809, 2299, 2331
Alw21I GWGCWC 3 cut(s) 1993, 2065, 2139
Alw26I GTCTC 4 cut(s) 407, 637, 1839, 2637
Alw44I GTGCAC 2 cut(s) 1989, 2135
AlwI GGATC 1 cut(s) 3463
AlwNI CAGNNNCTG 2 cut(s) 1948, 3214
Ama87I CYCGRG 1 cut(s) 3377
AoxI GGCC 2 cut(s) 252, 2254
ApaLI GTGCAC 2 cut(s) 1989, 2135
ApeKI GCWGC 7 cut(s) 215, 745, 1055, 2650, 2731, 3228, 3501
ApoI RAATTY 8 cut(s) 1158, 1250, 1512, 1999, 2244, 2272, 2375, 3235
Asp700I GAANNNNTTC 2 cut(s) 2379, 2721
AspLEI GCGC 4 cut(s) 1083, 1171, 2448, 2736
AspS9I GGNCC 1 cut(s) 3474
AsuC2I CCSGG 1 cut(s) 1977
AsuHPI GGTGA 7 cut(s) 12, 494, 1346, 2480, 3064, 3422, 3450
AsuII TTCGAA 2 cut(s) 1851, 3136
AvaI CYCGRG 1 cut(s) 3377
AvaII GGWCC 1 cut(s) 3474
BaeGI GKGCMC 2 cut(s) 1993, 2139
BanII GRGCYC 1 cut(s) 2065
BarI GAAGNNNNNNTAC 6 cut(s) 1371, 1403, 2653, 2685, 3010, 3042
BbsI GAAGAC 4 cut(s) 1332, 1481, 2766, 3027
Bbv12I GWGCWC 3 cut(s) 1993, 2065, 2139
BbvCI CCTCAGC 1 cut(s) 2176
BbvI GCAGC 7 cut(s) 227, 732, 1067, 2637, 2718, 3215, 3513
BceAI ACGGC 3 cut(s) 167, 267, 1115
BcgI CGANNNNNNTGC 2 cut(s) 3477, 3511
BciT130I CCWGG 4 cut(s) 381, 506, 2304, 2539
BciVI GTATCC 2 cut(s) 1005, 1406
BcnI CCSGG 1 cut(s) 1977
BcoDI GTCTC 4 cut(s) 407, 637, 1839, 2637
BfaI CTAG 5 cut(s) 417, 1994, 2766, 2924, 3332
BfmI CTRYAG 2 cut(s) 276, 3013
BfuAI ACCTGC 2 cut(s) 1174, 2586
BfuI GTATCC 2 cut(s) 1005, 1406
BisI GCNGC 8 cut(s) 216, 746, 1056, 1519, 2651, 2732, 3229, 3502
BlsI GCNGC 8 cut(s) 217, 747, 1057, 1520, 2652, 2733, 3230, 3503
Bme1390I CCNGG 5 cut(s) 381, 506, 1977, 2304, 2539
Bme18I GGWCC 1 cut(s) 3474
BmeT110I CYCGRG 1 cut(s) 3377
BmgT120I GGNCC 1 cut(s) 3474
BmiI GGNNCC 1 cut(s) 2308
BmrFI CCNGG 5 cut(s) 381, 506, 1977, 2304, 2539
BmrI ACTGGG 3 cut(s) 53, 814, 2125
BmuI ACTGGG 3 cut(s) 53, 814, 2125
BoxI GACNNNNGTC 1 cut(s) 629
BpiI GAAGAC 4 cut(s) 1332, 1481, 2766, 3027
BplI GAGNNNNNCTC 4 cut(s) 404, 436, 2720, 2752
BpmI CTGGAG 5 cut(s) 89, 2079, 2801, 2936, 3335
Bpu10I CCTNAGC 1 cut(s) 2176
Bpu14I TTCGAA 2 cut(s) 1851, 3136
BpuEI CTTGAG 6 cut(s) 191, 2393, 2596, 3083, 3172, 3404
BpuMI CCSGG 1 cut(s) 1977
BsaAI YACGTR 1 cut(s) 1391
BsaBI GATNNNNATC 1 cut(s) 2661
BsaJI CCNNGG 4 cut(s) 123, 2389, 3094, 3161
BsaWI WCCGGW 1 cut(s) 3460
Bsc4I CCNNNNNNNGG 5 cut(s) 907, 2390, 2595, 2862, 3095
Bse118I RCCGGY 2 cut(s) 1702, 1771
Bse1I ACTGG 6 cut(s) 59, 809, 1891, 2062, 2120, 3477
Bse3DI GCAATG 2 cut(s) 1109, 1863
Bse8I GATNNNNATC 1 cut(s) 2661
BseBI CCWGG 4 cut(s) 381, 506, 2304, 2539
BseDI CCNNGG 4 cut(s) 123, 2389, 3094, 3161
BseJI GATNNNNATC 1 cut(s) 2661
BseLI CCNNNNNNNGG 5 cut(s) 907, 2390, 2595, 2862, 3095
BseMI GCAATG 2 cut(s) 1109, 1863
BseMII CTCAG 5 cut(s) 486, 584, 2190, 2527, 3292
BseNI ACTGG 6 cut(s) 59, 809, 1891, 2062, 2120, 3477
BseRI GAGGAG 2 cut(s) 173, 993
BseSI GKGCMC 2 cut(s) 1993, 2139
BseXI GCAGC 7 cut(s) 227, 732, 1067, 2637, 2718, 3215, 3513
BseYI CCCAGC 1 cut(s) 945
BsgI GTGCAG 2 cut(s) 731, 2823
Bsh1236I CGCG 1 cut(s) 2446
BshFI GGCC 2 cut(s) 254, 2256
BsiHKAI GWGCWC 3 cut(s) 1993, 2065, 2139
BsiHKCI CYCGRG 1 cut(s) 3377
BsiSI CCGG 5 cut(s) 187, 1703, 1772, 1977, 3461
BslFI GGGAC 4 cut(s) 101, 821, 2586, 3360
BslI CCNNNNNNNGG 5 cut(s) 907, 2390, 2595, 2862, 3095
BsmAI GTCTC 4 cut(s) 407, 637, 1839, 2637
BsmFI GGGAC 4 cut(s) 101, 821, 2586, 3360
BsmI GAATGC 1 cut(s) 1220
BsnI GGCC 2 cut(s) 254, 2256
BsoBI CYCGRG 1 cut(s) 3377
Bsp119I TTCGAA 2 cut(s) 1851, 3136
Bsp1286I GDGCHC 3 cut(s) 1993, 2065, 2139
Bsp1407I TGTACA 1 cut(s) 3523
Bsp143I GATC 5 cut(s) 36, 657, 793, 1438, 3455
BspACI CCGC 8 cut(s) 592, 868, 1519, 2209, 2237, 2653, 3067, 3472
BspANI GGCC 2 cut(s) 254, 2256
BspCNI CTCAG 5 cut(s) 487, 583, 2189, 2526, 3291
BspFNI CGCG 1 cut(s) 2446
BspHI TCATGA 5 cut(s) 220, 660, 888, 1435, 3548
BspLI GGNNCC 1 cut(s) 2308
BspMAI CTGCAG 1 cut(s) 3017
BspMI ACCTGC 2 cut(s) 1174, 2586
BspPI GGATC 1 cut(s) 3463
BspQI GCTCTTC 2 cut(s) 445, 3511
BspT104I TTCGAA 2 cut(s) 1851, 3136
BsrBI CCGCTC 3 cut(s) 592, 1521, 3067
BsrDI GCAATG 2 cut(s) 1109, 1863
BsrFI RCCGGY 2 cut(s) 1702, 1771
BsrGI TGTACA 1 cut(s) 3523
BsrI ACTGG 6 cut(s) 59, 809, 1891, 2062, 2120, 3477
BssAI RCCGGY 2 cut(s) 1702, 1771
BssECI CCNNGG 4 cut(s) 123, 2389, 3094, 3161
BssMI GATC 5 cut(s) 36, 657, 793, 1438, 3455
BssT1I CCWWGG 4 cut(s) 123, 2389, 3094, 3161
Bst2UI CCWGG 4 cut(s) 381, 506, 2304, 2539
Bst6I CTCTTC 5 cut(s) 445, 981, 2226, 2640, 3511
BstAPI GCANNNNNTGC 1 cut(s) 745
BstAUI TGTACA 1 cut(s) 3523
BstBAI YACGTR 1 cut(s) 1391
BstBI TTCGAA 2 cut(s) 1851, 3136
BstC8I GCNNGC 7 cut(s) 75, 1197, 1316, 1704, 1773, 3270, 3531
BstDEI CTNAG 8 cut(s) 495, 570, 815, 1832, 1868, 2176, 2513, 3278
BstFNI CGCG 1 cut(s) 2446
BstHHI GCGC 4 cut(s) 1083, 1171, 2448, 2736
BstKTI GATC 5 cut(s) 39, 660, 796, 1441, 3458
BstMAI GTCTC 4 cut(s) 407, 637, 1839, 2637
BstMBI GATC 5 cut(s) 36, 657, 793, 1438, 3455
BstMWI GCNNNNNNNGC 8 cut(s) 202, 224, 575, 745, 2060, 2234, 2445, 2704
BstNI CCWGG 4 cut(s) 381, 506, 2304, 2539
BstNSI RCATGY 1 cut(s) 122
BstPAI GACNNNNGTC 1 cut(s) 629
BstSCI CCNGG 5 cut(s) 379, 504, 1975, 2302, 2537
BstSFI CTRYAG 2 cut(s) 276, 3013
BstSLI GKGCMC 2 cut(s) 1993, 2139
BstUI CGCG 1 cut(s) 2446
BstV1I GCAGC 7 cut(s) 227, 732, 1067, 2637, 2718, 3215, 3513
BstV2I GAAGAC 4 cut(s) 1332, 1481, 2766, 3027
BstXI CCANNNNNNTGG 1 cut(s) 1066
BsuI GTATCC 2 cut(s) 1005, 1406
BsuRI GGCC 2 cut(s) 254, 2256
BtsI GCAGTG 5 cut(s) 1946, 2810, 2835, 3215, 3412
BtsIMutI CAGTG 8 cut(s) 1946, 2521, 2709, 2810, 2835, 2840, 3215, 3412
BveI ACCTGC 2 cut(s) 1174, 2586
Cac8I GCNNGC 7 cut(s) 75, 1197, 1316, 1704, 1773, 3270, 3531
CaiI CAGNNNCTG 2 cut(s) 1948, 3214
CciI TCATGA 5 cut(s) 220, 660, 888, 1435, 3548
CfoI GCGC 4 cut(s) 1083, 1171, 2448, 2736
Cfr10I RCCGGY 2 cut(s) 1702, 1771
Cfr13I GGNCC 1 cut(s) 3474
CseI GACGC 1 cut(s) 2452
CsiI ACCWGGT 1 cut(s) 2537
Csp6I GTAC 8 cut(s) 384, 1046, 2188, 2475, 3082, 3448, 3492, 3524
CspCI CAANNNNNGTGG 2 cut(s) 2877, 2912
CviQI GTAC 8 cut(s) 384, 1046, 2188, 2475, 3082, 3448, 3492, 3524
DdeI CTNAG 8 cut(s) 495, 570, 815, 1832, 1868, 2176, 2513, 3278
DpnI GATC 5 cut(s) 38, 659, 795, 1440, 3457
DpnII GATC 5 cut(s) 36, 657, 793, 1438, 3455
DraI TTTAAA 3 cut(s) 921, 1087, 1809
DrdI GACNNNNNNGTC 1 cut(s) 3031
DseDI GACNNNNNNGTC 1 cut(s) 3031
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 5 cut(s) 445, 981, 2226, 2640, 3511
EarI CTCTTC 5 cut(s) 445, 981, 2226, 2640, 3511
Ecl136II GAGCTC 1 cut(s) 2063
Eco130I CCWWGG 4 cut(s) 123, 2389, 3094, 3161
Eco24I GRGCYC 1 cut(s) 2065
Eco32I GATATC 2 cut(s) 82, 761
Eco47I GGWCC 1 cut(s) 3474
Eco53kI GAGCTC 1 cut(s) 2063
Eco57I CTGAAG 4 cut(s) 810, 1925, 2099, 2250
Eco88I CYCGRG 1 cut(s) 3377
EcoICRI GAGCTC 1 cut(s) 2063
EcoRI GAATTC 1 cut(s) 1250
EcoRII CCWGG 4 cut(s) 379, 504, 2302, 2537
EcoRV GATATC 2 cut(s) 82, 761
EcoT14I CCWWGG 4 cut(s) 123, 2389, 3094, 3161
EcoT38I GRGCYC 1 cut(s) 2065
ErhI CCWWGG 4 cut(s) 123, 2389, 3094, 3161
FalI AAGNNNNNCTT 2 cut(s) 129, 161
FaqI GGGAC 4 cut(s) 101, 821, 2586, 3360
FauI CCCGC 3 cut(s) 585, 875, 2646
FauNDI CATATG 1 cut(s) 2483
FblI GTMKAC 1 cut(s) 3397
Fnu4HI GCNGC 8 cut(s) 216, 746, 1056, 1519, 2651, 2732, 3229, 3502
FriOI GRGCYC 1 cut(s) 2065
Fsp4HI GCNGC 8 cut(s) 216, 746, 1056, 1519, 2651, 2732, 3229, 3502
FspBI CTAG 5 cut(s) 417, 1994, 2766, 2924, 3332
GlaI GCGC 4 cut(s) 1082, 1170, 2447, 2735
GluI GCNGC 8 cut(s) 216, 746, 1056, 1519, 2651, 2732, 3229, 3502
GsaI CCCAGC 1 cut(s) 949
GsuI CTGGAG 5 cut(s) 89, 2079, 2801, 2936, 3335
HaeIII GGCC 2 cut(s) 254, 2256
HapII CCGG 5 cut(s) 187, 1703, 1772, 1977, 3461
HgaI GACGC 1 cut(s) 2452
HhaI GCGC 4 cut(s) 1083, 1171, 2448, 2736
Hin6I GCGC 4 cut(s) 1081, 1169, 2446, 2734
HinP1I GCGC 4 cut(s) 1081, 1169, 2446, 2734
HincII GTYRAC 2 cut(s) 2586, 2610
HindII GTYRAC 2 cut(s) 2586, 2610
HindIII AAGCTT 4 cut(s) 71, 2282, 2801, 3266
HpaI GTTAAC 1 cut(s) 2586
HpaII CCGG 5 cut(s) 187, 1703, 1772, 1977, 3461
HphI GGTGA 7 cut(s) 12, 494, 1346, 2480, 3064, 3422, 3450
HpyAV CCTTC 7 cut(s) 88, 245, 1301, 2826, 2999, 3143, 3176
HpyCH4IV ACGT 3 cut(s) 9, 1390, 3203
HpyF10VI GCNNNNNNNGC 8 cut(s) 202, 224, 575, 745, 2060, 2234, 2445, 2704
HpyF3I CTNAG 8 cut(s) 495, 570, 815, 1832, 1868, 2176, 2513, 3278
HpySE526I ACGT 3 cut(s) 9, 1390, 3203
HspAI GCGC 4 cut(s) 1081, 1169, 2446, 2734
KroI GCCGGC 2 cut(s) 1702, 1771
KroNI GCCGGC 2 cut(s) 1704, 1773
KspAI GTTAAC 1 cut(s) 2586
Kzo9I GATC 5 cut(s) 36, 657, 793, 1438, 3455
LguI GCTCTTC 2 cut(s) 445, 3511
LmnI GCTCC 4 cut(s) 160, 1232, 2060, 2647
Lsp1109I GCAGC 7 cut(s) 227, 732, 1067, 2637, 2718, 3215, 3513
MabI ACCWGGT 1 cut(s) 2537
MaeI CTAG 5 cut(s) 417, 1994, 2766, 2924, 3332
MaeII ACGT 3 cut(s) 9, 1390, 3203
MalI GATC 5 cut(s) 38, 659, 795, 1440, 3457
MbiI CCGCTC 3 cut(s) 592, 1521, 3067
MboI GATC 5 cut(s) 36, 657, 793, 1438, 3455
MfeI CAATTG 3 cut(s) 1407, 2359, 2970
MhlI GDGCHC 3 cut(s) 1993, 2065, 2139
MlyI GAGTC 9 cut(s) 443, 556, 686, 890, 1845, 2108, 2407, 2927, 3049
MmeI TCCRAC 2 cut(s) 625, 753
MroNI GCCGGC 2 cut(s) 1702, 1771
MroXI GAANNNNTTC 2 cut(s) 2379, 2721
MslI CAYNNNNRTG 4 cut(s) 507, 609, 3077, 3547
MspI CCGG 5 cut(s) 187, 1703, 1772, 1977, 3461
MspR9I CCNGG 5 cut(s) 381, 506, 1977, 2304, 2539
MunI CAATTG 3 cut(s) 1407, 2359, 2970
Mva1269I GAATGC 1 cut(s) 1220
MvaI CCWGG 4 cut(s) 381, 506, 2304, 2539
MvnI CGCG 1 cut(s) 2446
MwoI GCNNNNNNNGC 8 cut(s) 202, 224, 575, 745, 2060, 2234, 2445, 2704
NaeI GCCGGC 2 cut(s) 1704, 1773
NciI CCSGG 1 cut(s) 1977
NdeI CATATG 1 cut(s) 2483
NdeII GATC 5 cut(s) 36, 657, 793, 1438, 3455
NgoMIV GCCGGC 2 cut(s) 1702, 1771
NlaIV GGNNCC 1 cut(s) 2308
NmuCI GTSAC 6 cut(s) 500, 713, 1334, 2440, 2919, 2933
NspI RCATGY 1 cut(s) 122
NspV TTCGAA 2 cut(s) 1851, 3136
PagI TCATGA 5 cut(s) 220, 660, 888, 1435, 3548
PaqCI CACCTGC 1 cut(s) 2586
PciSI GCTCTTC 2 cut(s) 445, 3511
PctI GAATGC 1 cut(s) 1220
PdiI GCCGGC 2 cut(s) 1704, 1773
PdmI GAANNNNTTC 2 cut(s) 2379, 2721
PfeI GAWTC 5 cut(s) 1466, 1786, 1972, 2005, 3170
PflFI GACNNNGTC 1 cut(s) 308
PflMI CCANNNNNTGG 3 cut(s) 2390, 2862, 3095
PfoI TCCNGGA 1 cut(s) 2302
PkrI GCNGC 8 cut(s) 217, 747, 1057, 1520, 2652, 2733, 3230, 3503
PleI GAGTC 9 cut(s) 442, 556, 685, 890, 1844, 2107, 2407, 2926, 3048
PpsI GAGTC 9 cut(s) 442, 556, 685, 890, 1844, 2107, 2407, 2926, 3048
Ppu21I YACGTR 1 cut(s) 1391
PshAI GACNNNNGTC 1 cut(s) 629
PsiI TTATAA 1 cut(s) 1536
Psp124BI GAGCTC 1 cut(s) 2065
Psp6I CCWGG 4 cut(s) 379, 504, 2302, 2537
PspFI CCCAGC 1 cut(s) 945
PspGI CCWGG 4 cut(s) 379, 504, 2302, 2537
PspN4I GGNNCC 1 cut(s) 2308
PspPI GGNCC 1 cut(s) 3474
PstI CTGCAG 1 cut(s) 3017
PstNI CAGNNNCTG 2 cut(s) 1948, 3214
PsyI GACNNNGTC 1 cut(s) 308
RsaI GTAC 8 cut(s) 385, 1047, 2189, 2476, 3083, 3449, 3493, 3525
RsaNI GTAC 8 cut(s) 384, 1046, 2188, 2475, 3082, 3448, 3492, 3524
RseI CAYNNNNRTG 4 cut(s) 507, 609, 3077, 3547
SacI GAGCTC 1 cut(s) 2065
SapI GCTCTTC 2 cut(s) 445, 3511
SatI GCNGC 8 cut(s) 216, 746, 1056, 1519, 2651, 2732, 3229, 3502
Sau3AI GATC 5 cut(s) 36, 657, 793, 1438, 3455
Sau96I GGNCC 1 cut(s) 3474
SchI GAGTC 9 cut(s) 443, 556, 686, 890, 1845, 2108, 2407, 2927, 3049
ScrFI CCNGG 5 cut(s) 381, 506, 1977, 2304, 2539
SduI GDGCHC 3 cut(s) 1993, 2065, 2139
SexAI ACCWGGT 1 cut(s) 2537
SfcI CTRYAG 2 cut(s) 276, 3013
SfuI TTCGAA 2 cut(s) 1851, 3136
SinI GGWCC 1 cut(s) 3474
SmiMI CAYNNNNRTG 4 cut(s) 507, 609, 3077, 3547
SmlI CTYRAG 6 cut(s) 206, 2408, 2575, 3062, 3151, 3383
SmoI CTYRAG 6 cut(s) 206, 2408, 2575, 3062, 3151, 3383
SsiI CCGC 8 cut(s) 592, 868, 1519, 2209, 2237, 2653, 3067, 3472
SspI AATATT 3 cut(s) 2026, 2095, 2398
SspMI CTAG 5 cut(s) 417, 1994, 2766, 2924, 3332
SstI GAGCTC 1 cut(s) 2065
StyD4I CCNGG 5 cut(s) 379, 504, 1975, 2302, 2537
StyI CCWWGG 4 cut(s) 123, 2389, 3094, 3161
TaiI ACGT 3 cut(s) 12, 1393, 3206
TatI WGTACW 5 cut(s) 2474, 3081, 3447, 3491, 3523
TauI GCSGC 1 cut(s) 1521
TfiI GAWTC 5 cut(s) 1466, 1786, 1972, 2005, 3170
TscAI CASTG 8 cut(s) 1953, 2521, 2709, 2817, 2842, 2847, 3215, 3412
TseFI GTSAC 6 cut(s) 500, 713, 1334, 2440, 2919, 2933
TseI GCWGC 7 cut(s) 215, 745, 1055, 2650, 2731, 3228, 3501
Tsp45I GTSAC 6 cut(s) 500, 713, 1334, 2440, 2919, 2933
TspGWI ACGGA 1 cut(s) 1459
TspRI CASTG 8 cut(s) 1953, 2521, 2709, 2817, 2842, 2847, 3215, 3412
Tth111I GACNNNGTC 1 cut(s) 308
Van91I CCANNNNNTGG 3 cut(s) 2390, 2862, 3095
VneI GTGCAC 2 cut(s) 1989, 2135
VpaK11BI GGWCC 1 cut(s) 3474
XapI RAATTY 8 cut(s) 1158, 1250, 1512, 1999, 2244, 2272, 2375, 3235
XbaI TCTAGA 1 cut(s) 3331
XceI RCATGY 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 3397
XmnI GAANNNNTTC 2 cut(s) 2379, 2721
XspI CTAG 5 cut(s) 417, 1994, 2766, 2924, 3332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.