Rh6BG173500

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000400
Physical Location & Seq
Forward (+)
28601783 .. 28608130
6348 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG173500.1

Sequence Viewer

Length: 3594 bp
ATGGCAGACCTGGTCGTATCCCCAGCTTTGCAAGTGATCTTTGACAGAATAGCATCCCCCGTCATAGAAAAGTTTGCTGACTTGTGGGATTTCAAGGACAACCTCCAGAGGCTAAGAGAGACCTTGATGCTGATTCAACCTACTCTCGAGGATGCGGAAGAGCAACAATTCTCCAACAAAGCTGTCAAAATTTGGTTGTCAAAGCTCGAGAAGGCAGCTTATGATGCTGAGGACGGACTGCACTACTTGACTGCTAAAGGTATGATCCCTAGCAGTCGTTATCAACTTGATGATCTTAAGACTGCTTCCCACATTAAAGAGATGCTTCTGGCATTAGAAACAACTGTAAATGAGGGACTTGATAAGTTTACTTTTACAAGGCCTAATATGGTAGATCGTCGATCTTCAATGTGGGAGACTAGCTCTTTTGTCATCAAGTCACAGATATATGGAAGAGATGATGACAAAGATAAGGTTGTGAAACTGTTGCTGTCTTCTCAAGCTCACCGGGAAGGATATGCATCTTGTATCGCAATAGTTGGTATTGGAGGAATTGGTAAGACCACTCTTGCTCAATTAGCATATAATGACGAGAGAGTAATCCAAAACTTCGATGTTAGGATGTGGATTTATGTCTCTGATGATTTCAATGTCAAGAAGATCATGAAGACAATTATCGAGTCAGTAACCAATGATGAGTGCAGGTTCTCAGACATTGAACTTCTTCAGTCTCAACTTTGGCGTCTGCTGCACAATAAAAGATACCTCCTCGTGTTGGATGATGTTTGGACTTCGCATCACAATGACTGGGACAAACTAAAACCTTTGTTTAGAGGGGGTGTTGATGGATGCAAAGTCATTGTTACCACCCGCAATACAAAAACCGCACTCATGACAGACTCTCCAAATTCCCCTTTTTATTTGAAGGGCTTAGAGGAGGAGGATTGCTGGGCTTTGTTCAAGCAACGGGCTTTTGGGCGAGGAGAAGAAGAGAAATATCCACACCTTTTACCAATTGGTAAGCAGATTGTCAGAAAATGTGGAGGTGTGCCATTAGCAGCAAGAAGTTTGGGAAGTATAATGCGCTTCAAAAGAAATCAACAACAGTGGTTGTCTGTGCAAAATACTGAACTTTGGAAATTGGATGTGTGCGAACTAGACATTCTTCCTGCCCTGATGTTGAGTTACTTTTATCTACCATTTCATCTTAGAAGATGCTTTGCCTTCTGCTCAATGTTTCCAATAGGTTATGAATTCAGCAAGCACAAGTTAATTCATATGTGGATGGCGGAAGGCTTATTATTGGGGGAAGACTCGTGCAAGCGACCTGAAAATGTTGGTGATGACTACTTCACCAATTTGTTGTGGATGTCTTTCTTTCAAGAAGTAGATCGATGTGATGGTGGGGGTGTAATTGGGTACAAAATGAATGATGTCATTCATGGCCTCGCACAGTATGTTGCAGGGAACACAACCATGATACTTGAAAGTGGTGTCCGACCAAAGAATTTAGAAAAAGTTCGCCACTCCTCTGTTGTTTATAAATATAGATCAATCACAATTCCAGCTCTGTATGAAGCACAACACCTGCGAAGTCTGTTATTTATTGGAGAATCTGGCTTGATAAAGAACCTTCATACAATTTGCTCACGTTTAGTATACTTGCGTATGCTAGATCTGAACAATTGTGATGTTCATAAATTGCCGGAAACATTGTGCTACTTAATATGTTTGAGATATCTTGACCTGTCTTACACACCTATAGTCACGCTTCCTCATATCATGGCACAGAAACTCGTGTCTCTGCAGACTTTAAACCTACTTGGTTGCTCTAATCTGAGGCATTTGCCTGCCTCATTGGGACACATGACAGGCCTAAGACATCTCGATATAACTGCATGCACAAGTTTGACTGACATGCCTAGGGGTATTGGAAATCTATATCAACTTGAGACGTTGCCATTGTATGTTATTAGCTACAATGGCAGAGCCTTTGAGCTGGGTGGTTTGAATCTATATGGCAGGTTGAATCTCACACACTTGGAAAATATAACCAAGACAGTAGAAGCGAGATCAGCAAGATTGAAGATGAAGGAGAATATTGAGTCATTGGGACTATATTGGAGAGGCGAAGATGTGGATGAGTCAACCATGGTAAAACTACCTAAAGCCCAACAAGAAGCAGTCATCTCAGGATCTCAGAGAGTGCCACAGAAGTTTGGCCCGTCAGTCATAGACAGTCTACTTAGGGGTGTAATCAAAGAAGTCTCAGAGAATTCTGACACTGTGGAAGAAATTCTTGAAGCCTTGAAACCGCACGACAATTTGAAGATGCTAGTTATAAATGGGTATCCAGGAACTAGACTTCCTGATTGGACTCTCCCAAATCTAACTGCAGTTTATTTGAAGGAATGTAGAAATTGTCAGAATCTTCCAGCTCTTGGGAATCTCCTTGCGCTTAAAACTCTTTCTCTGCAAGGAATGGATAGTCTGAAACACGTCGGTGCAGATTTCTATGGTGATGGTACCAGCATTCCATTCTTGTCACTTGAAGAGTTATCACTCTGTGATTTATCCAACTTGGAAGAGTGGTCGAGTGCAAACAACATGGCTTCATTCCCGAGATTGAGGAAGTTAACCGTTAACAGATGTCCCAAGTTAGCACATATACAATTATCTGAATCTCTTCGATGTTTGGAGTTGCGGGGTTGTAAACTACATATGATGTGCATAGCAAATTTAAGTTTACTTTCTGTGCTCGTCTTAGAAAATATTCCAGACCTATCCTCTCTCCCAGAAGGACTTATTGCATCAGCTCATCTGTCTTGTCTGAAGATTTTGTCATGCCCCAAGCTTCGTTCACTGCCTTTGGAGATGGGAAATTGCCTTACTAGTCTGAAATCATTGACCATTGGTTGGTGTCAAGATCTTTCATCTCTGCCTGAGAGTTTGCAAAACCTCAAAGCCCTGGAATCACTGGAGATTAGTGACTGTCACTACATAATCTCCATGCCCGATGGTGCTATTGGAGGTTTGAGTTCCCTTCGAACTTTGTCCATTGAAAACTGCACTAGTCTGACTTCCTTGTCCTCAAGTTTGGAACATTTGACATTCCTTGAACACTTGACCATTATGTATTGTCCAAGTCTTGATTCTTTTCCAGAGGGTGTGCAACACCTCTCTGCCCTTCGAAGTTTGACTATCCTGAGCTGTCCTTGGTTTGACTCCCTGCCAAATGGCTTACAAAATGTCAGGACACTGCACTGTTTGGAAATTAGTAGCTGCACAAATCTAAGAGCTTTGCCAGAATGGTTTGAGAATCTTGACTCTCTTAGGTCCTTGACCATATCTGCTTGTCCTAATTTAGAAGAATTGCCGGCAGGTCTAAAGCATCTCACTAAACTCCAGTACCTCTCTATCCAAGAATGTCCTGAGCTTGAGCAAAGATGCAGACAAGGAAGTGGAGAGGATTGGTTCAAAATAGCTCATGTTCCACATAAATACATTGGTTCTCCTCAGGTCAGTCAATCCAGCGAAGCAAGCACCTCAGGCGGATCGTCTTCTCAAACATCTGCTCACGGCATGAGCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1197

Amino Acids

134.77

Weight (kDa)

6.21

Isoelectric Point (pI)

52.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 6 - 80 2.2e-17 Rx N-terminal domain
NB-ARC PF00931 153 - 326 4.9e-39 NB-ARC domain
WHD_DRP PF23559 412 - 484 1.1e-19 Disease resistance protein Winged helix domain
LRR_14 PF23598 529 - 746 4.1e-21 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 666 - 716 1.5e-06 R13L1/DRL21 LRRs
LRR_R13L1-DRL21 PF25019 757 - 828 2.1e-19 R13L1/DRL21 LRRs
LRR_14 PF23598 868 - 996 2.8e-06 Leucine-rich repeat region
LRR_13 PF23286 963 - 1149 1.2e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 1013 - 1142 4.6e-12 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 1080 - 1143 5e-07 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1542, 2342
AarI CACCTGC 1 cut(s) 1596
AasI GACNNNNNNGTC 1 cut(s) 3085
Acc36I ACCTGC 4 cut(s) 693, 1596, 2013, 3371
Acc65I GGTACC 1 cut(s) 2525
AccB1I GGYRCC 1 cut(s) 2525
AccB7I CCANNNNNTGG 2 cut(s) 2441, 2916
AccI GTMKAC 2 cut(s) 1659, 2242
AciI CCGC 7 cut(s) 155, 871, 885, 1289, 2315, 2704, 3552
AclWI GGATC 3 cut(s) 259, 2203, 3562
AcsI RAATTY 7 cut(s) 189, 907, 1253, 1507, 2275, 2295, 2737
AcuI CTGAAG 2 cut(s) 710, 2852
AcyI GRCGYC 1 cut(s) 742
AdeI CACNNNGTG 1 cut(s) 2567
AfaI GTAC 3 cut(s) 1421, 2527, 3410
AfiI CCNNNNNNNGG 3 cut(s) 775, 2441, 2916
AflII CTTAAG 1 cut(s) 296
AflIII ACRYGT 1 cut(s) 2497
AhlI ACTAGT 2 cut(s) 2891, 3071
AjiI CACGTC 1 cut(s) 2500
AjnI CCWGG 3 cut(s) 9, 2353, 2967
AjuI GAANNNNNNNTTGG 2 cut(s) 3136, 3168
AleI CACNNNNGTG 1 cut(s) 2502
AloI GAACNNNNNNTCC 2 cut(s) 2350, 2382
Alw21I GWGCWC 1 cut(s) 2760
Alw26I GTCTC 7 cut(s) 113, 410, 640, 735, 1806, 1946, 2272
AlwI GGATC 3 cut(s) 259, 2203, 3562
Ama87I CYCGRG 3 cut(s) 146, 206, 2620
AoxI GGCC 4 cut(s) 380, 1444, 1873, 2221
ApeKI GCWGC 4 cut(s) 215, 748, 1058, 3282
ApoI RAATTY 7 cut(s) 189, 907, 1253, 1507, 2275, 2295, 2737
Asp700I GAANNNNTTC 5 cut(s) 723, 1518, 2295, 2685, 2772
Asp718I GGTACC 1 cut(s) 2525
AspA2I CCTAGG 1 cut(s) 1922
AspLEI GCGC 2 cut(s) 1086, 2458
AspS9I GGNCC 2 cut(s) 2222, 3336
AsuC2I CCSGG 1 cut(s) 509
AsuHPI GGTGA 4 cut(s) 497, 1345, 1352, 2531
AsuII TTCGAA 2 cut(s) 3046, 3190
AvaI CYCGRG 3 cut(s) 146, 206, 2620
AvaII GGWCC 1 cut(s) 3336
AvrII CCTAGG 1 cut(s) 1922
AxyI CCTNAGG 2 cut(s) 3516, 3547
BaeI ACNNNNGTAYC 2 cut(s) 3392, 3425
BanI GGYRCC 1 cut(s) 2525
BauI CACGAG 3 cut(s) 770, 1315, 1796
BbsI GAAGAC 4 cut(s) 486, 674, 1317, 3552
Bbv12I GWGCWC 1 cut(s) 2760
BbvCI CCTCAGC 1 cut(s) 228
BbvI GCAGC 4 cut(s) 227, 735, 1070, 3269
BccI CCATC 6 cut(s) 839, 1279, 1394, 2516, 2869, 3011
BcgI CGANNNNNNTGC 2 cut(s) 1877, 1911
BciT130I CCWGG 3 cut(s) 11, 2355, 2969
BciVI GTATCC 2 cut(s) 28, 2361
BcnI CCSGG 1 cut(s) 509
BcoDI GTCTC 7 cut(s) 113, 410, 640, 735, 1806, 1946, 2272
BcuI ACTAGT 2 cut(s) 2891, 3071
BfaI CTAG 9 cut(s) 270, 420, 1157, 1673, 1923, 2336, 2361, 2892, 3072
BfmI CTRYAG 3 cut(s) 1761, 1805, 2394
BfrI CTTAAG 1 cut(s) 296
BfuAI ACCTGC 4 cut(s) 693, 1596, 2013, 3371
BfuI GTATCC 2 cut(s) 28, 2361
BglII AGATCT 2 cut(s) 1675, 2926
BisI GCNGC 4 cut(s) 216, 749, 1059, 3283
BlnI CCTAGG 1 cut(s) 1922
BlsI GCNGC 4 cut(s) 217, 750, 1060, 3284
Bme1390I CCNGG 4 cut(s) 11, 509, 2355, 2969
Bme18I GGWCC 1 cut(s) 3336
BmeT110I CYCGRG 3 cut(s) 146, 206, 2620
BmgBI CACGTC 1 cut(s) 2500
BmgT120I GGNCC 2 cut(s) 2222, 3336
BmiI GGNNCC 1 cut(s) 2527
BmrFI CCNGG 4 cut(s) 11, 509, 2355, 2969
BmrI ACTGGG 1 cut(s) 817
BmuI ACTGGG 1 cut(s) 817
BpiI GAAGAC 4 cut(s) 486, 674, 1317, 3552
BplI GAGNNNNNCTC 2 cut(s) 407, 439
BpmI CTGGAG 3 cut(s) 89, 2999, 3389
Bpu10I CCTNAGC 3 cut(s) 228, 3206, 3432
Bpu14I TTCGAA 2 cut(s) 3046, 3190
BpuEI CTTGAG 4 cut(s) 483, 1970, 3076, 3458
BpuMI CCSGG 1 cut(s) 509
Bsa29I ATCGAT 1 cut(s) 1393
BsaBI GATNNNNATC 1 cut(s) 520
BsaHI GRCGYC 1 cut(s) 742
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 4 cut(s) 1922, 2151, 2967, 3215
BsaXI ACNNNNNCTCC 4 cut(s) 886, 916, 2990, 3020
Bsc4I CCNNNNNNNGG 3 cut(s) 775, 2441, 2916
Bse118I RCCGGY 1 cut(s) 3376
Bse1I ACTGG 3 cut(s) 812, 2982, 3406
Bse21I CCTNAGG 2 cut(s) 3516, 3547
Bse8I GATNNNNATC 1 cut(s) 520
BseBI CCWGG 3 cut(s) 11, 2355, 2969
BseCI ATCGAT 1 cut(s) 1393
BseDI CCNNGG 4 cut(s) 1922, 2151, 2967, 3215
BseGI GGATG 9 cut(s) 53, 157, 627, 784, 854, 1150, 1290, 1374, 2146
BseJI GATNNNNATC 1 cut(s) 520
BseLI CCNNNNNNNGG 3 cut(s) 775, 2441, 2916
BseNI ACTGG 3 cut(s) 812, 2982, 3406
BseRI GAGGAG 6 cut(s) 758, 950, 953, 996, 1519, 3504
BseXI GCAGC 4 cut(s) 227, 735, 1070, 3269
BseYI CCCAGC 3 cut(s) 22, 948, 1999
BsgI GTGCAG 7 cut(s) 224, 721, 734, 2526, 3052, 3245, 3268
BshFI GGCC 4 cut(s) 382, 1446, 1875, 2223
BshNI GGYRCC 1 cut(s) 2525
BshVI ATCGAT 1 cut(s) 1393
BsiHKAI GWGCWC 1 cut(s) 2760
BsiHKCI CYCGRG 3 cut(s) 146, 206, 2620
BsiSI CCGG 3 cut(s) 508, 1706, 3377
BslFI GGGAC 5 cut(s) 369, 824, 1875, 2127, 2637
BslI CCNNNNNNNGG 3 cut(s) 775, 2441, 2916
BsmAI GTCTC 7 cut(s) 113, 410, 640, 735, 1806, 1946, 2272
BsmBI CGTCTC 1 cut(s) 1946
BsmFI GGGAC 5 cut(s) 369, 824, 1875, 2127, 2637
BsmI GAATGC 1 cut(s) 2532
BsnI GGCC 4 cut(s) 382, 1446, 1875, 2223
Bso31I GGTCTC 1 cut(s) 113
BsoBI CYCGRG 3 cut(s) 146, 206, 2620
Bsp119I TTCGAA 2 cut(s) 3046, 3190
Bsp1286I GDGCHC 1 cut(s) 2760
Bsp19I CCATGG 1 cut(s) 2151
BspACI CCGC 7 cut(s) 155, 871, 885, 1289, 2315, 2704, 3552
BspANI GGCC 4 cut(s) 382, 1446, 1875, 2223
BspDI ATCGAT 1 cut(s) 1393
BspHI TCATGA 2 cut(s) 663, 891
BspLI GGNNCC 1 cut(s) 2527
BspMAI CTGCAG 2 cut(s) 1809, 2398
BspMI ACCTGC 4 cut(s) 693, 1596, 2013, 3371
BspPI GGATC 3 cut(s) 259, 2203, 3562
BspQI GCTCTTC 1 cut(s) 153
BspT104I TTCGAA 2 cut(s) 3046, 3190
BspT107I GGYRCC 1 cut(s) 2525
BspTI CTTAAG 1 cut(s) 296
BspTNI GGTCTC 1 cut(s) 113
BsrFI RCCGGY 1 cut(s) 3376
BsrI ACTGG 3 cut(s) 812, 2982, 3406
BssAI RCCGGY 1 cut(s) 3376
BssECI CCNNGG 4 cut(s) 1922, 2151, 2967, 3215
BssNAI GTATAC 1 cut(s) 1660
BssNI GRCGYC 1 cut(s) 742
BssSI CACGAG 3 cut(s) 770, 1315, 1796
BssT1I CCWWGG 3 cut(s) 1922, 2151, 3215
Bst1107I GTATAC 1 cut(s) 1660
Bst2BI CACGAG 3 cut(s) 770, 1315, 1796
Bst2UI CCWGG 3 cut(s) 11, 2355, 2969
Bst6I CTCTTC 6 cut(s) 153, 448, 984, 2547, 2580, 2691
BstACI GRCGYC 1 cut(s) 742
BstAFI CTTAAG 1 cut(s) 296
BstBI TTCGAA 2 cut(s) 3046, 3190
BstC8I GCNNGC 6 cut(s) 1262, 1322, 1851, 1900, 3378, 3541
BstDSI CCRYGG 1 cut(s) 2151
BstF5I GGATG 9 cut(s) 53, 157, 627, 784, 854, 1150, 1290, 1374, 2146
BstHHI GCGC 2 cut(s) 1086, 2458
BstMAI GTCTC 7 cut(s) 113, 410, 640, 735, 1806, 1946, 2272
BstMWI GCNNNNNNNGC 7 cut(s) 224, 578, 748, 1983, 2075, 3540, 3549
BstNI CCWGG 3 cut(s) 11, 2355, 2969
BstNSI RCATGY 2 cut(s) 1902, 1921
BstSCI CCNGG 4 cut(s) 9, 507, 2353, 2967
BstSFI CTRYAG 3 cut(s) 1761, 1805, 2394
BstV1I GCAGC 4 cut(s) 227, 735, 1070, 3269
BstV2I GAAGAC 4 cut(s) 486, 674, 1317, 3552
BstX2I RGATCY 3 cut(s) 1675, 2195, 2926
BstYI RGATCY 3 cut(s) 1675, 2195, 2926
BstZ17I GTATAC 1 cut(s) 1660
Bsu15I ATCGAT 1 cut(s) 1393
Bsu36I CCTNAGG 2 cut(s) 3516, 3547
BsuI GTATCC 2 cut(s) 28, 2361
BsuRI GGCC 4 cut(s) 382, 1446, 1875, 2223
BsuTUI ATCGAT 1 cut(s) 1393
BtgI CCRYGG 1 cut(s) 2151
BtrI CACGTC 1 cut(s) 2500
BtsCI GGATG 9 cut(s) 53, 157, 627, 784, 854, 1150, 1290, 1374, 2146
BtsI GCAGTG 2 cut(s) 2861, 3257
BtsIMutI CAGTG 6 cut(s) 1112, 2283, 2861, 2975, 3257, 3262
BveI ACCTGC 4 cut(s) 693, 1596, 2013, 3371
Cac8I GCNNGC 6 cut(s) 1262, 1322, 1851, 1900, 3378, 3541
CciI TCATGA 2 cut(s) 663, 891
CfoI GCGC 2 cut(s) 1086, 2458
Cfr10I RCCGGY 1 cut(s) 3376
Cfr13I GGNCC 2 cut(s) 2222, 3336
ClaI ATCGAT 1 cut(s) 1393
CseI GACGC 1 cut(s) 731
CsiI ACCWGGT 1 cut(s) 9
Csp6I GTAC 3 cut(s) 1420, 2526, 3409
CspCI CAANNNNNGTGG 2 cut(s) 1088, 1123
CviQI GTAC 3 cut(s) 1420, 2526, 3409
DraI TTTAAA 1 cut(s) 1815
DraIII CACNNNGTG 1 cut(s) 2567
DrdI GACNNNNNNGTC 1 cut(s) 3085
DseDI GACNNNNNNGTC 1 cut(s) 3085
Eam1104I CTCTTC 6 cut(s) 153, 448, 984, 2547, 2580, 2691
EarI CTCTTC 6 cut(s) 153, 448, 984, 2547, 2580, 2691
EciI GGCGGA 2 cut(s) 1304, 3567
Eco130I CCWWGG 3 cut(s) 1922, 2151, 3215
Eco147I AGGCCT 2 cut(s) 382, 1875
Eco31I GGTCTC 1 cut(s) 113
Eco32I GATATC 1 cut(s) 1739
Eco47I GGWCC 1 cut(s) 3336
Eco57I CTGAAG 2 cut(s) 710, 2852
Eco81I CCTNAGG 2 cut(s) 3516, 3547
Eco88I CYCGRG 3 cut(s) 146, 206, 2620
EcoO109I RGGNCCY 1 cut(s) 3336
EcoRI GAATTC 2 cut(s) 1253, 2275
EcoRII CCWGG 3 cut(s) 9, 2353, 2967
EcoRV GATATC 1 cut(s) 1739
EcoT14I CCWWGG 3 cut(s) 1922, 2151, 3215
EcoT22I ATGCAT 1 cut(s) 523
ErhI CCWWGG 3 cut(s) 1922, 2151, 3215
Esp3I CGTCTC 1 cut(s) 1946
FalI AAGNNNNNCTT 4 cut(s) 309, 341, 2283, 2315
FaqI GGGAC 5 cut(s) 369, 824, 1875, 2127, 2637
FauI CCCGC 2 cut(s) 878, 2697
FauNDI CATATG 2 cut(s) 1278, 2721
FblI GTMKAC 2 cut(s) 1659, 2242
Fnu4HI GCNGC 4 cut(s) 216, 749, 1059, 3283
FokI GGATG 9 cut(s) 40, 164, 634, 791, 861, 1157, 1297, 1381, 2153
Fsp4HI GCNGC 4 cut(s) 216, 749, 1059, 3283
FspBI CTAG 9 cut(s) 270, 420, 1157, 1673, 1923, 2336, 2361, 2892, 3072
GlaI GCGC 2 cut(s) 1085, 2457
GluI GCNGC 4 cut(s) 216, 749, 1059, 3283
GsaI CCCAGC 3 cut(s) 26, 952, 2003
GsuI CTGGAG 3 cut(s) 89, 2999, 3389
HaeIII GGCC 4 cut(s) 382, 1446, 1875, 2223
HapII CCGG 3 cut(s) 508, 1706, 3377
HgaI GACGC 1 cut(s) 731
HhaI GCGC 2 cut(s) 1086, 2458
Hin1I GRCGYC 1 cut(s) 742
Hin6I GCGC 2 cut(s) 1084, 2456
HinP1I GCGC 2 cut(s) 1084, 2456
HincII GTYRAC 3 cut(s) 2148, 2637, 2644
HindII GTYRAC 3 cut(s) 2148, 2637, 2644
HindIII AAGCTT 1 cut(s) 2852
HpaI GTTAAC 2 cut(s) 2637, 2644
HpaII CCGG 3 cut(s) 508, 1706, 3377
HphI GGTGA 4 cut(s) 497, 1345, 1352, 2531
Hpy166II GTNNAC 9 cut(s) 369, 1660, 2148, 2243, 2637, 2644, 2714, 2747, 2861
Hpy8I GTNNAC 9 cut(s) 369, 1660, 2148, 2243, 2637, 2644, 2714, 2747, 2861
Hpy99I CGWCG 2 cut(s) 402, 2504
HpyCH4IV ACGT 3 cut(s) 1651, 1955, 2499
HpyF10VI GCNNNNNNNGC 7 cut(s) 224, 578, 748, 1983, 2075, 3540, 3549
HpySE526I ACGT 3 cut(s) 1651, 1955, 2499
Hsp92I GRCGYC 1 cut(s) 742
HspAI GCGC 2 cut(s) 1084, 2456
KpnI GGTACC 1 cut(s) 2529
KroI GCCGGC 1 cut(s) 3376
KroNI GCCGGC 1 cut(s) 3378
KspAI GTTAAC 2 cut(s) 2637, 2644
LguI GCTCTTC 1 cut(s) 153
Lsp1109I GCAGC 4 cut(s) 227, 735, 1070, 3269
MabI ACCWGGT 1 cut(s) 9
MaeI CTAG 9 cut(s) 270, 420, 1157, 1673, 1923, 2336, 2361, 2892, 3072
MaeII ACGT 3 cut(s) 1651, 1955, 2499
MaeIII GTNAC 8 cut(s) 438, 685, 862, 1184, 1765, 2544, 2987, 2993
MfeI CAATTG 2 cut(s) 1014, 1684
MflI RGATCY 3 cut(s) 1675, 2195, 2926
MhlI GDGCHC 1 cut(s) 2760
MlyI GAGTC 8 cut(s) 689, 893, 1307, 2114, 2153, 2371, 3218, 3320
MmeI TCCRAC 4 cut(s) 198, 756, 1522, 2601
Mph1103I ATGCAT 1 cut(s) 523
MroNI GCCGGC 1 cut(s) 3376
MroXI GAANNNNTTC 5 cut(s) 723, 1518, 2295, 2685, 2772
MseI TTAA 9 cut(s) 297, 315, 1271, 1724, 1814, 2460, 2636, 2643, 2741
MslI CAYNNNNRTG 3 cut(s) 801, 1475, 2502
MspCI CTTAAG 1 cut(s) 296
MspI CCGG 3 cut(s) 508, 1706, 3377
MspR9I CCNGG 4 cut(s) 11, 509, 2355, 2969
MunI CAATTG 2 cut(s) 1014, 1684
Mva1269I GAATGC 1 cut(s) 2532
MvaI CCWGG 3 cut(s) 11, 2355, 2969
MwoI GCNNNNNNNGC 7 cut(s) 224, 578, 748, 1983, 2075, 3540, 3549
NaeI GCCGGC 1 cut(s) 3378
NciI CCSGG 1 cut(s) 509
NcoI CCATGG 1 cut(s) 2151
NdeI CATATG 2 cut(s) 1278, 2721
NgoMIV GCCGGC 1 cut(s) 3376
NlaIV GGNNCC 1 cut(s) 2527
NmuCI GTSAC 5 cut(s) 438, 1765, 2544, 2987, 2993
NsiI ATGCAT 1 cut(s) 523
NspI RCATGY 2 cut(s) 1902, 1921
NspV TTCGAA 2 cut(s) 3046, 3190
OliI CACNNNNGTG 1 cut(s) 2502
PaeI GCATGC 1 cut(s) 1902
PaeR7I CTCGAG 2 cut(s) 146, 206
PagI TCATGA 2 cut(s) 663, 891
PaqCI CACCTGC 1 cut(s) 1596
PceI AGGCCT 2 cut(s) 382, 1875
PciSI GCTCTTC 1 cut(s) 153
PctI GAATGC 1 cut(s) 2532
PdiI GCCGGC 1 cut(s) 3378
PdmI GAANNNNTTC 5 cut(s) 723, 1518, 2295, 2685, 2772
PflFI GACNNNGTC 1 cut(s) 11
PflMI CCANNNNNTGG 2 cut(s) 2441, 2916
PfoI TCCNGGA 1 cut(s) 2353
PkrI GCNGC 4 cut(s) 217, 750, 1060, 3284
PleI GAGTC 8 cut(s) 688, 893, 1307, 2113, 2152, 2371, 3218, 3320
PpsI GAGTC 8 cut(s) 688, 893, 1307, 2113, 2152, 2371, 3218, 3320
PpuMI RGGWCCY 1 cut(s) 3336
PsiI TTATAA 2 cut(s) 1542, 2342
Psp5II RGGWCCY 1 cut(s) 3336
Psp6I CCWGG 3 cut(s) 9, 2353, 2967
PspFI CCCAGC 3 cut(s) 22, 948, 1999
PspGI CCWGG 3 cut(s) 9, 2353, 2967
PspN4I GGNNCC 1 cut(s) 2527
PspPI GGNCC 2 cut(s) 2222, 3336
PspPPI RGGWCCY 1 cut(s) 3336
PstI CTGCAG 2 cut(s) 1809, 2398
PsuI RGATCY 3 cut(s) 1675, 2195, 2926
PsyI GACNNNGTC 1 cut(s) 11
RsaI GTAC 3 cut(s) 1421, 2527, 3410
RsaNI GTAC 3 cut(s) 1420, 2526, 3409
RseI CAYNNNNRTG 3 cut(s) 801, 1475, 2502
SapI GCTCTTC 1 cut(s) 153
SaqAI TTAA 9 cut(s) 297, 315, 1271, 1724, 1814, 2460, 2636, 2643, 2741
SatI GCNGC 4 cut(s) 216, 749, 1059, 3283
Sau96I GGNCC 2 cut(s) 2222, 3336
SchI GAGTC 8 cut(s) 689, 893, 1307, 2114, 2153, 2371, 3218, 3320
ScrFI CCNGG 4 cut(s) 11, 509, 2355, 2969
SduI GDGCHC 1 cut(s) 2760
SexAI ACCWGGT 1 cut(s) 9
SfcI CTRYAG 3 cut(s) 1761, 1805, 2394
Sfr274I CTCGAG 2 cut(s) 146, 206
SfuI TTCGAA 2 cut(s) 3046, 3190
SinI GGWCC 1 cut(s) 3336
SlaI CTCGAG 2 cut(s) 146, 206
SmiMI CAYNNNNRTG 3 cut(s) 801, 1475, 2502
SmlI CTYRAG 7 cut(s) 146, 206, 296, 498, 1949, 3091, 3437
SmoI CTYRAG 7 cut(s) 146, 206, 296, 498, 1949, 3091, 3437
SpeI ACTAGT 2 cut(s) 2891, 3071
SphI GCATGC 1 cut(s) 1902
SseBI AGGCCT 2 cut(s) 382, 1875
SsiI CCGC 7 cut(s) 155, 871, 885, 1289, 2315, 2704, 3552
SspI AATATT 2 cut(s) 2101, 2773
SspMI CTAG 9 cut(s) 270, 420, 1157, 1673, 1923, 2336, 2361, 2892, 3072
StuI AGGCCT 2 cut(s) 382, 1875
StyD4I CCNGG 4 cut(s) 9, 507, 2353, 2967
StyI CCWWGG 3 cut(s) 1922, 2151, 3215
TaiI ACGT 3 cut(s) 1654, 1958, 2502
Tru1I TTAA 9 cut(s) 297, 315, 1271, 1724, 1814, 2460, 2636, 2643, 2741
Tru9I TTAA 9 cut(s) 297, 315, 1271, 1724, 1814, 2460, 2636, 2643, 2741
TscAI CASTG 6 cut(s) 1112, 2290, 2868, 2982, 3264, 3269
TseFI GTSAC 5 cut(s) 438, 1765, 2544, 2987, 2993
TseI GCWGC 4 cut(s) 215, 748, 1058, 3282
Tsp45I GTSAC 5 cut(s) 438, 1765, 2544, 2987, 2993
TspGWI ACGGA 1 cut(s) 249
TspRI CASTG 6 cut(s) 1112, 2290, 2868, 2982, 3264, 3269
Tth111I GACNNNGTC 1 cut(s) 11
Van91I CCANNNNNTGG 2 cut(s) 2441, 2916
Vha464I CTTAAG 1 cut(s) 296
VpaK11BI GGWCC 1 cut(s) 3336
XapI RAATTY 7 cut(s) 189, 907, 1253, 1507, 2275, 2295, 2737
XceI RCATGY 2 cut(s) 1902, 1921
XhoI CTCGAG 2 cut(s) 146, 206
XmaJI CCTAGG 1 cut(s) 1922
XmiI GTMKAC 2 cut(s) 1659, 2242
XmnI GAANNNNTTC 5 cut(s) 723, 1518, 2295, 2685, 2772
XspI CTAG 9 cut(s) 270, 420, 1157, 1673, 1923, 2336, 2361, 2892, 3072
Zsp2I ATGCAT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.