Rw1G018040

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000400
Physical Location & Seq
Reverse (-)
39746242 .. 39749784
3543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G018040.1

Sequence Viewer

Length: 3543 bp
ATGGCAGACGTAGTTTTATCACCAGCTTTGCAAGTGATCTTTGACAGAGTAGCATCCCCAGTCCTACAAAAGCTTGCTGATATCTGGGACATGAAGGACAACCTCCAGAGCCTACAACATGCCTTGGTCATGGTTCAAGCTATTCTTGAAGATGCAGAGGAGCAACAACTGACCAACAAAGCCGTCCGGCTTTGGCTGTTGAAGCTCAAGAACGCAGCTCATGATGCTGATGACCTTCTGGATTTCGTTACGGCCAAAGGAAAAGATAAGTGGACTTTAGCAATTTCAAAATCCATAGATGCTGACAAAGTCAGAAAGATGCTTTTGACATTAGAAATGACAGTAAACGAGGGTCTATTGAAGTTTAATTTTCGAGAGCCTGGTACAGTAGATAGGCAATCTGATAAAAGGGAGACTAGCTCTTTTGTTATGGAGTCAGAAATATATGGAAGAGCTGATGACAAAGAGAAGTTAGTGAAACTCCTACTATCTTCTGAGTGTCACCAGGATGGTTATGCTATGTGTATTCCGATAATTGGTATTGGAGGAATTGGCAAGACGACTCTTGCTCAGTTGGCATATAATGATGAGCGGGTAATCCAACATTTTGATGTTAGGCTGTGGACTTTTGTCTCGCATGATTTCAATATCAAGAAGATCATGAAGTCAATTATTGAGTCTGTAACAAGGGAAGATTGCAAGTTCTCGGAGAGTGACTTACTTCAATCTCGACTTTGGCATTTGCTGCACAACAAAAGATATCTAATTGTGTTGGATGATGTTTGGACTGAAGATCAAGATGAGTGGGACAAACTAAGACCTTTGTTTAGACGAGGTGTTGATGGATGCAGAATCATTATCACTTCCCGCAATAAAAAAATCCCATTCATGATGGACTCCCTAACTTCGGCATACTATTTAAAGGGTTTGGTGGAGGATGACTGCTGGGCTTTGTTCAAGCAACGGGCTTTTGGACGAGGAGAAGAAGAGAAGTATCCCGACCTCTGCCTGATTGGAAAGCAGATTGCAAAGAAATGTGGGGGTGTACCATTAGCAGCTAAAAGTTTGGGAAGTGTTATGCGCTTTAAAAGAGAAGAACGGCAATGGTTGTTTATGCAAAATAGTGAACTTTGGGAAGTAGATGGATGCAAACAGAGAATTTTACCTGCGCTTATGTTAAGTTATCTTCATCTGCCAGCACATCTTCGACAATGCTTTGCATTCTGCTCCATATTTCCTAAAAATTATGAATTCAAGAAGCATAAATTAGTTCTCTTGTGGATGGCAGAGGGCTTAATTCTAGAAGAAGGTAGCAAGCGACCCGAAGACATTGGTGATGAATACTTTTCTGATTTGCTGTGGATGTCTTTCTTTCAAGAAGTAGAGTTACGTGATGGTGGAAGCACAATTGGATACAAAATGAATGATGTCATTCATGATCTTGAACGATATGTTTCTGGAAATGAATCCGTTATGCTTGAACAAGGTCTTCCACCAAGTAATCCAGCACAAATTCGCCGCTCATCCATTATTTATAAATACAGGGAAGTTACAATTCCAGAAGCTCTCTTTGAAGCAGAACATTTACGAACTGTCTTATTGATTGGAGAATATGGACTTCTAAAAGACAGTAGTAAAATGTTATTTAATTGTATATACCTGCGTGTGCTGGACTTGAACAACTGTGAAGTTCATTTTTTGCCGTCATCAATAGGTGGGTTGATATGTTTGAGGTATCTCGACTTGTCTTATACGCCTATCTCCAAGTTGCCGGCAACCACAAGGAATCTCTGTTCATTGCAGACTTTAAACCTCTCTGGATGTCATAATCTTAGAGTCTTGCGAGACTTCGAAGCAATGACCAGCCTAAGACATCTCAATATAACTGGATGTGATAGCTTGTGGTATATATCTCCTTGTATTAAAATGTTACTTCAACTTCAGACACTGCCATTATATTTCGTTCGCAAGAATCCCGGTAATCTTGGTGCACTAGAGAATTTGAATCTTTATGGGGAGTTGAATATTACACACTTGGAGAATATAAGAAATGCAACTGGAGCTCAATCATCTGGACTGAAGATGAAAGAAAATATTGAGTCGTTGGGATTATACTGGGGAGATTTTGGAGATGTGCACGATAGGAATAAATCACTATTGAAGAAACCAGCCAGGTCCTCAGCCAAGTCCTCAGCCAAATGGTACTGTTCAGAAGTAGGGATGCGGTGGCATGAACCTAATGCTGAAGAGGCGGTAGAAATTCTTGATGGCCTGAAACCACACAAGAATTTGAAAAAGCTAGTTATACATGGTTATCCAGGAATCAGATTTCCAGATTGGAAACTGCCAAATCTAATTGCAGTTGATTTGTCCAATTGTAAGAACTGTGAATATCTTCCAGCCCTTGGGAATCTTCTTCTACTCAAGACTCTTTCTCTGCGTGGAATGGAGAGCGTGACGCGCATTGGCTTTGAGTTCCATGGTGATAGTACAGATATTTGCTTTTCATCACTTGAAGAATTATCGCTCAGTGATTTTCCGAAATTGGAAACCTGGTCGAGTGCAAATAATGAAAATGCATTTCCGAGCTTGAGGAAGTTAACCATACGTAGGTGTCCCAAGTTGACACAGATACCATTTTGTTTGTCTCTTCAACATTTGGAGCTGCGGGATTGTAATCCATCTTCAATCTTCATTGGAGGTTTAACTTTGCTGACAGTGCTTGTAATAGAAAAACTTCCACAGCTGCGCACTCTACCACAAGGGCTTTCTGCATCAGTTAGTCTGTCTTCTCTGGAGGTCTTATCTTGCCCCAAGCTTTGTTCACTGCCTTCGGAGATGGGAAACCTCACTGCACTGAAATCATTGACCATTCGTTGGTGTGATGAGTTGTCCTCTCTGCCACAGAGTTTGCAGAACCTCAAAGCTCTGGAATCACTAGAGATTAGTGACTGTCATAGTATAATCTCGATGCCAGATGGTGCAATTGGAGGTTTGAGTTCCCTTCAAACTTTGTCCATTGAGAACTGCAGTAGTCTGACTTCTTTGTCTTCGAGTCTGGAACAACTCGCTTTTCTTGAGCGGTTCACCATTATGTACTGTCCAAACCTTGGTTCTTTCCCAGAGGGTGTGCGACACCTCTCAACCCTTCGAACTTTGTCTATCTTGAGCTGCCCTTGGTTTGATTCTTTGCCAGAAGGGTTGCAAATGGTCAAGACGTTGCAGTGTCTGGAAATTAGTAGCTGCCCGAATTTCACAGCTTTGCCAGAGTGGGTTGAGAAGCTTGCTTCACTCAGATCTTTGACCATTTTTGATTGTCCTAATTTGAAACTGTTGCCACAAAGTCTAGAACTTCTCACTAAACTCCAGCACCTCTCTATTCAAGAATGTCCCGAGCTTGAGGAAAGATGTAGACAAGGCAGTGGTGAGGATTGGCTGAAAATAGCTCATGTTCCACATAAGTACATTGGATCGCTGGAGATGAGGCGGTCCAGTAGTGAAGCAAGTACATCGGGCAGCTCTTCTGTTGAAGCATCTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1180

Amino Acids

133.51

Weight (kDa)

6.14

Isoelectric Point (pI)

54.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 85 1.1e-16 Rx N-terminal domain
NB-ARC PF00931 153 - 325 1.9e-36 NB-ARC domain
WHD_DRP PF23559 411 - 481 4.9e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 548 - 887 2.1e-17 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 663 - 816 3.7e-26 R13L1/DRL21 LRRs
LRR_14 PF23598 860 - 983 3.1e-07 Leucine-rich repeat region
LRR_14 PF23598 933 - 1031 3.7e-08 Leucine-rich repeat region
LRR_14 PF23598 1036 - 1130 5.9e-08 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 1066 - 1130 9.5e-06 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1536
AasI GACNNNNNNGTC 1 cut(s) 3046
Acc16I TGCGCA 1 cut(s) 2750
Acc36I ACCTGC 2 cut(s) 1174, 1668
AccB7I CCANNNNNTGG 3 cut(s) 2405, 2877, 3110
AccBSI CCGCTC 3 cut(s) 592, 1521, 3082
AccI GTMKAC 1 cut(s) 3412
AccII CGCG 1 cut(s) 2461
AciI CCGC 8 cut(s) 592, 868, 1519, 2224, 2252, 2668, 3082, 3487
AclWI GGATC 1 cut(s) 3478
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 7 cut(s) 1158, 1250, 1512, 1999, 2259, 2287, 3250
AcuI CTGAAG 4 cut(s) 810, 1925, 2099, 2265
AfaI GTAC 7 cut(s) 385, 1047, 2204, 2491, 3098, 3464, 3508
AfiI CCNNNNNNNGG 6 cut(s) 536, 907, 2405, 2610, 2877, 3110
AjnI CCWGG 5 cut(s) 379, 504, 2171, 2317, 2552
AjuI GAANNNNNNNTTGG 2 cut(s) 3097, 3129
AloI GAACNNNNNNTCC 2 cut(s) 1777, 1809
Alw21I GWGCWC 3 cut(s) 1993, 2065, 2139
Alw26I GTCTC 4 cut(s) 407, 637, 1839, 2652
Alw44I GTGCAC 2 cut(s) 1989, 2135
AlwI GGATC 1 cut(s) 3478
AlwNI CAGNNNCTG 2 cut(s) 1948, 3229
Ama87I CYCGRG 1 cut(s) 3392
AoxI GGCC 2 cut(s) 252, 2269
ApaLI GTGCAC 2 cut(s) 1989, 2135
ApeKI GCWGC 8 cut(s) 215, 745, 1055, 2665, 2746, 3171, 3243, 3516
ApoI RAATTY 7 cut(s) 1158, 1250, 1512, 1999, 2259, 2287, 3250
Asp700I GAANNNNTTC 2 cut(s) 2394, 2736
AspLEI GCGC 4 cut(s) 1083, 1171, 2463, 2751
AspS9I GGNCC 2 cut(s) 2175, 3489
AsuC2I CCSGG 1 cut(s) 1977
AsuHPI GGTGA 6 cut(s) 12, 494, 1346, 2495, 3079, 3437
AsuII TTCGAA 2 cut(s) 1851, 3151
AvaI CYCGRG 1 cut(s) 3392
AvaII GGWCC 2 cut(s) 2175, 3489
BaeGI GKGCMC 2 cut(s) 1993, 2139
BanII GRGCYC 1 cut(s) 2065
BarI GAAGNNNNNNTAC 6 cut(s) 1371, 1403, 2668, 2700, 3025, 3057
BbsI GAAGAC 4 cut(s) 1332, 1481, 2781, 3042
Bbv12I GWGCWC 3 cut(s) 1993, 2065, 2139
BbvCI CCTCAGC 2 cut(s) 2179, 2191
BbvI GCAGC 8 cut(s) 227, 732, 1067, 2652, 2733, 3158, 3230, 3528
BceAI ACGGC 4 cut(s) 167, 267, 1115, 1687
BcgI CGANNNNNNTGC 2 cut(s) 3492, 3526
BciT130I CCWGG 5 cut(s) 381, 506, 2173, 2319, 2554
BciVI GTATCC 2 cut(s) 1005, 1406
BcnI CCSGG 1 cut(s) 1977
BcoDI GTCTC 4 cut(s) 407, 637, 1839, 2652
BfaI CTAG 6 cut(s) 417, 1301, 1994, 2300, 2939, 3347
BfmI CTRYAG 1 cut(s) 3028
BfuAI ACCTGC 2 cut(s) 1174, 1668
BfuI GTATCC 2 cut(s) 1005, 1406
BglII AGATCT 1 cut(s) 3296
BisI GCNGC 9 cut(s) 216, 746, 1056, 1519, 2666, 2747, 3172, 3244, 3517
BlsI GCNGC 9 cut(s) 217, 747, 1057, 1520, 2667, 2748, 3173, 3245, 3518
Bme1390I CCNGG 6 cut(s) 381, 506, 1977, 2173, 2319, 2554
Bme18I GGWCC 2 cut(s) 2175, 3489
BmeT110I CYCGRG 1 cut(s) 3392
BmgT120I GGNCC 2 cut(s) 2175, 3489
BmrFI CCNGG 6 cut(s) 381, 506, 1977, 2173, 2319, 2554
BmrI ACTGGG 2 cut(s) 53, 2125
BmuI ACTGGG 2 cut(s) 53, 2125
BoxI GACNNNNGTC 1 cut(s) 629
BpiI GAAGAC 4 cut(s) 1332, 1481, 2781, 3042
BplI GAGNNNNNCTC 4 cut(s) 404, 436, 2879, 2911
BpmI CTGGAG 5 cut(s) 89, 2079, 2816, 3350, 3497
Bpu10I CCTNAGC 2 cut(s) 2179, 2191
Bpu14I TTCGAA 2 cut(s) 1851, 3151
BpuEI CTTGAG 6 cut(s) 191, 2408, 2611, 3098, 3187, 3419
BpuMI CCSGG 1 cut(s) 1977
BsaAI YACGTR 2 cut(s) 1391, 2609
BsaBI GATNNNNATC 1 cut(s) 2676
BsaJI CCNNGG 5 cut(s) 123, 2404, 2479, 3109, 3176
Bsc4I CCNNNNNNNGG 6 cut(s) 536, 907, 2405, 2610, 2877, 3110
Bse118I RCCGGY 1 cut(s) 1771
Bse1I ACTGG 5 cut(s) 59, 1891, 2062, 2120, 3492
Bse3DI GCAATG 3 cut(s) 1109, 1796, 1863
Bse8I GATNNNNATC 1 cut(s) 2676
BseBI CCWGG 5 cut(s) 381, 506, 2173, 2319, 2554
BseDI CCNNGG 5 cut(s) 123, 2404, 2479, 3109, 3176
BseJI GATNNNNATC 1 cut(s) 2676
BseLI CCNNNNNNNGG 6 cut(s) 536, 907, 2405, 2610, 2877, 3110
BseMI GCAATG 3 cut(s) 1109, 1796, 1863
BseMII CTCAG 6 cut(s) 486, 584, 2193, 2205, 2542, 3307
BseNI ACTGG 5 cut(s) 59, 1891, 2062, 2120, 3492
BseRI GAGGAG 2 cut(s) 173, 993
BseSI GKGCMC 2 cut(s) 1993, 2139
BseXI GCAGC 8 cut(s) 227, 732, 1067, 2652, 2733, 3158, 3230, 3528
BseYI CCCAGC 1 cut(s) 945
BsgI GTGCAG 2 cut(s) 731, 2838
Bsh1236I CGCG 1 cut(s) 2461
BshFI GGCC 2 cut(s) 254, 2271
BsiHKAI GWGCWC 3 cut(s) 1993, 2065, 2139
BsiHKCI CYCGRG 1 cut(s) 3392
BsiSI CCGG 3 cut(s) 187, 1772, 1977
BslFI GGGAC 4 cut(s) 101, 821, 2601, 3375
BslI CCNNNNNNNGG 6 cut(s) 536, 907, 2405, 2610, 2877, 3110
BsmAI GTCTC 4 cut(s) 407, 637, 1839, 2652
BsmFI GGGAC 4 cut(s) 101, 821, 2601, 3375
BsmI GAATGC 1 cut(s) 1220
BsnI GGCC 2 cut(s) 254, 2271
BsoBI CYCGRG 1 cut(s) 3392
Bsp119I TTCGAA 2 cut(s) 1851, 3151
Bsp1286I GDGCHC 3 cut(s) 1993, 2065, 2139
Bsp143I GATC 6 cut(s) 36, 657, 793, 1438, 3296, 3470
Bsp19I CCATGG 1 cut(s) 2479
BspACI CCGC 8 cut(s) 592, 868, 1519, 2224, 2252, 2668, 3082, 3487
BspANI GGCC 2 cut(s) 254, 2271
BspCNI CTCAG 6 cut(s) 487, 583, 2192, 2204, 2541, 3306
BspFNI CGCG 1 cut(s) 2461
BspHI TCATGA 4 cut(s) 220, 660, 888, 1435
BspMAI CTGCAG 1 cut(s) 3032
BspMI ACCTGC 2 cut(s) 1174, 1668
BspPI GGATC 1 cut(s) 3478
BspQI GCTCTTC 2 cut(s) 445, 3526
BspT104I TTCGAA 2 cut(s) 1851, 3151
BsrBI CCGCTC 3 cut(s) 592, 1521, 3082
BsrDI GCAATG 3 cut(s) 1109, 1796, 1863
BsrFI RCCGGY 1 cut(s) 1771
BsrI ACTGG 5 cut(s) 59, 1891, 2062, 2120, 3492
BssAI RCCGGY 1 cut(s) 1771
BssECI CCNNGG 5 cut(s) 123, 2404, 2479, 3109, 3176
BssMI GATC 6 cut(s) 36, 657, 793, 1438, 3296, 3470
BssT1I CCWWGG 5 cut(s) 123, 2404, 2479, 3109, 3176
Bst2UI CCWGG 5 cut(s) 381, 506, 2173, 2319, 2554
Bst6I CTCTTC 5 cut(s) 445, 981, 2241, 2655, 3526
BstAPI GCANNNNNTGC 1 cut(s) 745
BstBAI YACGTR 2 cut(s) 1391, 2609
BstBI TTCGAA 2 cut(s) 1851, 3151
BstC8I GCNNGC 5 cut(s) 75, 1197, 1316, 1773, 3285
BstDEI CTNAG 9 cut(s) 495, 570, 815, 1832, 1868, 2179, 2191, 2528, 3293
BstDSI CCRYGG 1 cut(s) 2479
BstFNI CGCG 1 cut(s) 2461
BstHHI GCGC 4 cut(s) 1083, 1171, 2463, 2751
BstKTI GATC 6 cut(s) 39, 660, 796, 1441, 3299, 3473
BstMAI GTCTC 4 cut(s) 407, 637, 1839, 2652
BstMBI GATC 6 cut(s) 36, 657, 793, 1438, 3296, 3470
BstMWI GCNNNNNNNGC 8 cut(s) 202, 224, 575, 745, 2060, 2249, 2460, 2719
BstNI CCWGG 5 cut(s) 381, 506, 2173, 2319, 2554
BstNSI RCATGY 1 cut(s) 122
BstPAI GACNNNNGTC 1 cut(s) 629
BstSCI CCNGG 6 cut(s) 379, 504, 1975, 2171, 2317, 2552
BstSFI CTRYAG 1 cut(s) 3028
BstSLI GKGCMC 2 cut(s) 1993, 2139
BstSNI TACGTA 1 cut(s) 2609
BstUI CGCG 1 cut(s) 2461
BstV1I GCAGC 8 cut(s) 227, 732, 1067, 2652, 2733, 3158, 3230, 3528
BstV2I GAAGAC 4 cut(s) 1332, 1481, 2781, 3042
BstX2I RGATCY 1 cut(s) 3296
BstYI RGATCY 1 cut(s) 3296
BsuI GTATCC 2 cut(s) 1005, 1406
BsuRI GGCC 2 cut(s) 254, 2271
BtgI CCRYGG 1 cut(s) 2479
BtsI GCAGTG 5 cut(s) 1946, 2825, 2850, 3230, 3427
BtsIMutI CAGTG 8 cut(s) 1946, 2536, 2724, 2825, 2850, 2855, 3230, 3427
BveI ACCTGC 2 cut(s) 1174, 1668
Cac8I GCNNGC 5 cut(s) 75, 1197, 1316, 1773, 3285
CaiI CAGNNNCTG 2 cut(s) 1948, 3229
CciI TCATGA 4 cut(s) 220, 660, 888, 1435
CfoI GCGC 4 cut(s) 1083, 1171, 2463, 2751
Cfr10I RCCGGY 1 cut(s) 1771
Cfr13I GGNCC 2 cut(s) 2175, 3489
CseI GACGC 1 cut(s) 2467
CsiI ACCWGGT 1 cut(s) 2552
Csp6I GTAC 7 cut(s) 384, 1046, 2203, 2490, 3097, 3463, 3507
CspCI CAANNNNNGTGG 4 cut(s) 785, 820, 2892, 2927
CviQI GTAC 7 cut(s) 384, 1046, 2203, 2490, 3097, 3463, 3507
DdeI CTNAG 9 cut(s) 495, 570, 815, 1832, 1868, 2179, 2191, 2528, 3293
DpnI GATC 6 cut(s) 38, 659, 795, 1440, 3298, 3472
DpnII GATC 6 cut(s) 36, 657, 793, 1438, 3296, 3470
DraI TTTAAA 3 cut(s) 921, 1087, 1809
DrdI GACNNNNNNGTC 1 cut(s) 3046
DseDI GACNNNNNNGTC 1 cut(s) 3046
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 5 cut(s) 445, 981, 2241, 2655, 3526
EarI CTCTTC 5 cut(s) 445, 981, 2241, 2655, 3526
Ecl136II GAGCTC 1 cut(s) 2063
Eco105I TACGTA 1 cut(s) 2609
Eco130I CCWWGG 5 cut(s) 123, 2404, 2479, 3109, 3176
Eco24I GRGCYC 1 cut(s) 2065
Eco32I GATATC 2 cut(s) 82, 761
Eco47I GGWCC 2 cut(s) 2175, 3489
Eco53kI GAGCTC 1 cut(s) 2063
Eco57I CTGAAG 4 cut(s) 810, 1925, 2099, 2265
Eco88I CYCGRG 1 cut(s) 3392
EcoICRI GAGCTC 1 cut(s) 2063
EcoO109I RGGNCCY 1 cut(s) 2175
EcoRI GAATTC 1 cut(s) 1250
EcoRII CCWGG 5 cut(s) 379, 504, 2171, 2317, 2552
EcoRV GATATC 2 cut(s) 82, 761
EcoT14I CCWWGG 5 cut(s) 123, 2404, 2479, 3109, 3176
EcoT22I ATGCAT 1 cut(s) 2581
EcoT38I GRGCYC 1 cut(s) 2065
ErhI CCWWGG 5 cut(s) 123, 2404, 2479, 3109, 3176
FalI AAGNNNNNCTT 2 cut(s) 129, 161
FaqI GGGAC 4 cut(s) 101, 821, 2601, 3375
FauI CCCGC 3 cut(s) 585, 875, 2661
FblI GTMKAC 1 cut(s) 3412
Fnu4HI GCNGC 9 cut(s) 216, 746, 1056, 1519, 2666, 2747, 3172, 3244, 3517
FriOI GRGCYC 1 cut(s) 2065
Fsp4HI GCNGC 9 cut(s) 216, 746, 1056, 1519, 2666, 2747, 3172, 3244, 3517
FspBI CTAG 6 cut(s) 417, 1301, 1994, 2300, 2939, 3347
FspI TGCGCA 1 cut(s) 2750
GlaI GCGC 4 cut(s) 1082, 1170, 2462, 2750
GluI GCNGC 9 cut(s) 216, 746, 1056, 1519, 2666, 2747, 3172, 3244, 3517
GsaI CCCAGC 1 cut(s) 949
GsuI CTGGAG 5 cut(s) 89, 2079, 2816, 3350, 3497
HaeIII GGCC 2 cut(s) 254, 2271
HapII CCGG 3 cut(s) 187, 1772, 1977
HgaI GACGC 1 cut(s) 2467
HhaI GCGC 4 cut(s) 1083, 1171, 2463, 2751
Hin6I GCGC 4 cut(s) 1081, 1169, 2461, 2749
HinP1I GCGC 4 cut(s) 1081, 1169, 2461, 2749
HincII GTYRAC 2 cut(s) 2601, 2625
HindII GTYRAC 2 cut(s) 2601, 2625
HindIII AAGCTT 3 cut(s) 71, 2816, 3281
HpaI GTTAAC 1 cut(s) 2601
HpaII CCGG 3 cut(s) 187, 1772, 1977
HphI GGTGA 6 cut(s) 12, 494, 1346, 2495, 3079, 3437
HpyAV CCTTC 7 cut(s) 88, 245, 1301, 2841, 3014, 3158, 3191
HpyCH4IV ACGT 4 cut(s) 9, 1390, 2608, 3218
HpyF10VI GCNNNNNNNGC 8 cut(s) 202, 224, 575, 745, 2060, 2249, 2460, 2719
HpyF3I CTNAG 9 cut(s) 495, 570, 815, 1832, 1868, 2179, 2191, 2528, 3293
HpySE526I ACGT 4 cut(s) 9, 1390, 2608, 3218
HspAI GCGC 4 cut(s) 1081, 1169, 2461, 2749
KroI GCCGGC 1 cut(s) 1771
KroNI GCCGGC 1 cut(s) 1773
KspAI GTTAAC 1 cut(s) 2601
Kzo9I GATC 6 cut(s) 36, 657, 793, 1438, 3296, 3470
LguI GCTCTTC 2 cut(s) 445, 3526
LmnI GCTCC 4 cut(s) 160, 1232, 2060, 2662
Lsp1109I GCAGC 8 cut(s) 227, 732, 1067, 2652, 2733, 3158, 3230, 3528
MabI ACCWGGT 1 cut(s) 2552
MaeI CTAG 6 cut(s) 417, 1301, 1994, 2300, 2939, 3347
MaeII ACGT 4 cut(s) 9, 1390, 2608, 3218
MaeIII GTNAC 9 cut(s) 247, 500, 682, 713, 1386, 1549, 1929, 2455, 2948
MalI GATC 6 cut(s) 38, 659, 795, 1440, 3298, 3472
MbiI CCGCTC 3 cut(s) 592, 1521, 3082
MboI GATC 6 cut(s) 36, 657, 793, 1438, 3296, 3470
MfeI CAATTG 3 cut(s) 1407, 2374, 2985
MflI RGATCY 1 cut(s) 3296
MhlI GDGCHC 3 cut(s) 1993, 2065, 2139
MlyI GAGTC 8 cut(s) 443, 556, 686, 890, 1845, 2108, 2422, 3064
MmeI TCCRAC 2 cut(s) 625, 753
Mph1103I ATGCAT 1 cut(s) 2581
MroNI GCCGGC 1 cut(s) 1771
MroXI GAANNNNTTC 2 cut(s) 2394, 2736
MslI CAYNNNNRTG 3 cut(s) 507, 609, 3092
MspA1I CMGCKG 1 cut(s) 2746
MspI CCGG 3 cut(s) 187, 1772, 1977
MspR9I CCNGG 6 cut(s) 381, 506, 1977, 2173, 2319, 2554
MunI CAATTG 3 cut(s) 1407, 2374, 2985
Mva1269I GAATGC 1 cut(s) 1220
MvaI CCWGG 5 cut(s) 381, 506, 2173, 2319, 2554
MvnI CGCG 1 cut(s) 2461
MwoI GCNNNNNNNGC 8 cut(s) 202, 224, 575, 745, 2060, 2249, 2460, 2719
NaeI GCCGGC 1 cut(s) 1773
NciI CCSGG 1 cut(s) 1977
NcoI CCATGG 1 cut(s) 2479
NdeII GATC 6 cut(s) 36, 657, 793, 1438, 3296, 3470
NgoMIV GCCGGC 1 cut(s) 1771
NmuCI GTSAC 4 cut(s) 500, 713, 2455, 2948
NsbI TGCGCA 1 cut(s) 2750
NsiI ATGCAT 1 cut(s) 2581
NspI RCATGY 1 cut(s) 122
NspV TTCGAA 2 cut(s) 1851, 3151
PagI TCATGA 4 cut(s) 220, 660, 888, 1435
PciSI GCTCTTC 2 cut(s) 445, 3526
PctI GAATGC 1 cut(s) 1220
PdiI GCCGGC 1 cut(s) 1773
PdmI GAANNNNTTC 2 cut(s) 2394, 2736
PfeI GAWTC 9 cut(s) 852, 1466, 1786, 1972, 2005, 2322, 2410, 2933, 3185
PflFI GACNNNGTC 1 cut(s) 308
PflMI CCANNNNNTGG 3 cut(s) 2405, 2877, 3110
PfoI TCCNGGA 1 cut(s) 2317
PkrI GCNGC 9 cut(s) 217, 747, 1057, 1520, 2667, 2748, 3173, 3245, 3518
PleI GAGTC 8 cut(s) 442, 556, 685, 890, 1844, 2107, 2422, 3063
PpsI GAGTC 8 cut(s) 442, 556, 685, 890, 1844, 2107, 2422, 3063
Ppu21I YACGTR 2 cut(s) 1391, 2609
PpuMI RGGWCCY 1 cut(s) 2175
PshAI GACNNNNGTC 1 cut(s) 629
PsiI TTATAA 1 cut(s) 1536
Psp124BI GAGCTC 1 cut(s) 2065
Psp5II RGGWCCY 1 cut(s) 2175
Psp6I CCWGG 5 cut(s) 379, 504, 2171, 2317, 2552
PspFI CCCAGC 1 cut(s) 945
PspGI CCWGG 5 cut(s) 379, 504, 2171, 2317, 2552
PspPI GGNCC 2 cut(s) 2175, 3489
PspPPI RGGWCCY 1 cut(s) 2175
PstI CTGCAG 1 cut(s) 3032
PstNI CAGNNNCTG 2 cut(s) 1948, 3229
PsuI RGATCY 1 cut(s) 3296
PsyI GACNNNGTC 1 cut(s) 308
PvuII CAGCTG 1 cut(s) 2746
RsaI GTAC 7 cut(s) 385, 1047, 2204, 2491, 3098, 3464, 3508
RsaNI GTAC 7 cut(s) 384, 1046, 2203, 2490, 3097, 3463, 3507
RseI CAYNNNNRTG 3 cut(s) 507, 609, 3092
SacI GAGCTC 1 cut(s) 2065
SapI GCTCTTC 2 cut(s) 445, 3526
SatI GCNGC 9 cut(s) 216, 746, 1056, 1519, 2666, 2747, 3172, 3244, 3517
Sau3AI GATC 6 cut(s) 36, 657, 793, 1438, 3296, 3470
Sau96I GGNCC 2 cut(s) 2175, 3489
SchI GAGTC 8 cut(s) 443, 556, 686, 890, 1845, 2108, 2422, 3064
ScrFI CCNGG 6 cut(s) 381, 506, 1977, 2173, 2319, 2554
SduI GDGCHC 3 cut(s) 1993, 2065, 2139
SexAI ACCWGGT 1 cut(s) 2552
SfcI CTRYAG 1 cut(s) 3028
SfuI TTCGAA 2 cut(s) 1851, 3151
SinI GGWCC 2 cut(s) 2175, 3489
SmiMI CAYNNNNRTG 3 cut(s) 507, 609, 3092
SmlI CTYRAG 6 cut(s) 206, 2423, 2590, 3077, 3166, 3398
SmoI CTYRAG 6 cut(s) 206, 2423, 2590, 3077, 3166, 3398
SnaBI TACGTA 1 cut(s) 2609
SsiI CCGC 8 cut(s) 592, 868, 1519, 2224, 2252, 2668, 3082, 3487
SspI AATATT 2 cut(s) 2026, 2095
SspMI CTAG 6 cut(s) 417, 1301, 1994, 2300, 2939, 3347
SstI GAGCTC 1 cut(s) 2065
StyD4I CCNGG 6 cut(s) 379, 504, 1975, 2171, 2317, 2552
StyI CCWWGG 5 cut(s) 123, 2404, 2479, 3109, 3176
TaiI ACGT 4 cut(s) 12, 1393, 2611, 3221
TaqI TCGA 9 cut(s) 373, 730, 1207, 1740, 1851, 2558, 2969, 3053, 3151
TatI WGTACW 4 cut(s) 2489, 3096, 3462, 3506
TauI GCSGC 1 cut(s) 1521
TfiI GAWTC 9 cut(s) 852, 1466, 1786, 1972, 2005, 2322, 2410, 2933, 3185
TscAI CASTG 8 cut(s) 1953, 2536, 2724, 2832, 2857, 2862, 3230, 3427
TseFI GTSAC 4 cut(s) 500, 713, 2455, 2948
TseI GCWGC 8 cut(s) 215, 745, 1055, 2665, 2746, 3171, 3243, 3516
Tsp45I GTSAC 4 cut(s) 500, 713, 2455, 2948
TspGWI ACGGA 1 cut(s) 1459
TspRI CASTG 8 cut(s) 1953, 2536, 2724, 2832, 2857, 2862, 3230, 3427
Tth111I GACNNNGTC 1 cut(s) 308
Van91I CCANNNNNTGG 3 cut(s) 2405, 2877, 3110
VneI GTGCAC 2 cut(s) 1989, 2135
VpaK11BI GGWCC 2 cut(s) 2175, 3489
XapI RAATTY 7 cut(s) 1158, 1250, 1512, 1999, 2259, 2287, 3250
XbaI TCTAGA 2 cut(s) 1300, 3346
XceI RCATGY 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 3412
XmnI GAANNNNTTC 2 cut(s) 2394, 2736
XspI CTAG 6 cut(s) 417, 1301, 1994, 2300, 2939, 3347
Zsp2I ATGCAT 1 cut(s) 2581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.