RchiOBHm_Chr6g0269421

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000400
Physical Location & Seq
Forward (+)
26730539 .. 26731394
856 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24169

Sequence Viewer

Length: 822 bp
ATGGCAGACCTGGTCGTATCCCCAGCTTTGCAAGTGATCTTTGACAGAATAGCATCCCCTGTCATAGAAAAGTTTGCTGACTTGTGGGATTTCAAGGACAACCTCCAGAGGCTAAGAGAGACCTTGATGCTGATTCAACCTACTCTCGAGGATGCGGAAGAGCAACAATTCTCCAACAAAGCTGTCAAAATTTGGTTGTCAAAGCTCGAGAAGGCAGCTTATGATGCTGAGGACGGACTGCACTACTTGACTGCTAAAGGTATGATCCCTAGCAGTCGTTATCAACTTGATGATCTTAAGACTGCTTCCCACATTAAAGAGATGCTTCTGGCATTAGAAACAACTGTAAATGAGGGACTTGTTAAGTTTACTTTTACAAGGCCTAATATGGTAGATCGTCGATCTTCCGTGTGGGAGACTAGCTCTTTTGTCATCAAGTCACAGATATATGGAAGAGATCAAGACAAAGATAAGGTCGTGAAACTGTTGCTGTCTTCTCAAGCTCACCAGGAAGGATATGCATCTTGTATCGCAATAGTTGGTATTGGAGGAATTGGTAAGACCACTCTTGCTCAATTAGCATATAATGACGAGAGAGTAATCCAAAACTTCGATGTTAGGATGTGGATTTATGTCTCTGATGATTTCAATGTCAAGAAGATCATGAAGACAATTATCGAGTCAGTAACCAATGATGAGTGCAGGTTCTCAGACATTGAACTTCTTCAGTCTCAGCTTTGGCGTCTGCTGCACAATAAAAGATACCTCCTCGTGTTGGATGATGTTTGGACTTCGCATCACAATGACTGGGACATTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

273

Amino Acids

31.49

Weight (kDa)

5.32

Isoelectric Point (pI)

47.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 6 - 80 2.2e-18 Rx N-terminal domain
NB-ARC PF00931 153 - 271 4.8e-27 NB-ARC domain
AAA_22 PF13401 173 - 262 5.1e-06 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 693
AciI CCGC 1 cut(s) 155
AclWI GGATC 1 cut(s) 259
AcsI RAATTY 1 cut(s) 189
AcuI CTGAAG 1 cut(s) 710
AcyI GRCGYC 1 cut(s) 742
AfiI CCNNNNNNNGG 1 cut(s) 775
AflII CTTAAG 1 cut(s) 296
AgsI TTSAA 4 cut(s) 94, 137, 649, 719
AjnI CCWGG 2 cut(s) 9, 507
AluBI AGCT 7 cut(s) 26, 182, 205, 218, 423, 503, 736
AluI AGCT 7 cut(s) 26, 182, 205, 218, 423, 503, 736
Alw26I GTCTC 4 cut(s) 113, 410, 640, 735
AlwI GGATC 1 cut(s) 259
Ama87I CYCGRG 2 cut(s) 146, 206
AoxI GGCC 1 cut(s) 380
ApeKI GCWGC 2 cut(s) 215, 748
ApoI RAATTY 1 cut(s) 189
ArsI GACNNNNNNTTYG 2 cut(s) 459, 491
Asp700I GAANNNNTTC 1 cut(s) 723
AsuHPI GGTGA 1 cut(s) 497
AvaI CYCGRG 2 cut(s) 146, 206
BauI CACGAG 1 cut(s) 770
BbsI GAAGAC 2 cut(s) 486, 674
BbvCI CCTCAGC 1 cut(s) 228
BbvI GCAGC 2 cut(s) 227, 735
BciT130I CCWGG 2 cut(s) 11, 509
BciVI GTATCC 1 cut(s) 28
BcoDI GTCTC 4 cut(s) 113, 410, 640, 735
BfaI CTAG 2 cut(s) 270, 420
BfrI CTTAAG 1 cut(s) 296
BfuAI ACCTGC 1 cut(s) 693
BfuI GTATCC 1 cut(s) 28
BisI GCNGC 2 cut(s) 216, 749
BlsI GCNGC 2 cut(s) 217, 750
Bme1390I CCNGG 2 cut(s) 11, 509
BmeT110I CYCGRG 2 cut(s) 146, 206
BmrFI CCNGG 2 cut(s) 11, 509
BmrI ACTGGG 1 cut(s) 817
BmsI GCATC 7 cut(s) 62, 117, 142, 214, 312, 530, 805
BmuI ACTGGG 1 cut(s) 817
BpiI GAAGAC 2 cut(s) 486, 674
BplI GAGNNNNNCTC 2 cut(s) 407, 439
BpmI CTGGAG 1 cut(s) 89
Bpu10I CCTNAGC 1 cut(s) 228
BpuEI CTTGAG 1 cut(s) 483
BsaBI GATNNNNATC 1 cut(s) 520
BsaHI GRCGYC 1 cut(s) 742
BsaI GGTCTC 1 cut(s) 113
Bsc4I CCNNNNNNNGG 1 cut(s) 775
Bse1I ACTGG 1 cut(s) 812
Bse8I GATNNNNATC 1 cut(s) 520
BseBI CCWGG 2 cut(s) 11, 509
BseGI GGATG 4 cut(s) 53, 157, 627, 784
BseJI GATNNNNATC 1 cut(s) 520
BseLI CCNNNNNNNGG 1 cut(s) 775
BseMII CTCAG 3 cut(s) 219, 723, 746
BseNI ACTGG 1 cut(s) 812
BseRI GAGGAG 1 cut(s) 758
BseXI GCAGC 2 cut(s) 227, 735
BseYI CCCAGC 1 cut(s) 22
BsgI GTGCAG 3 cut(s) 224, 721, 734
BshFI GGCC 1 cut(s) 382
BsiHKCI CYCGRG 2 cut(s) 146, 206
BslFI GGGAC 1 cut(s) 369
BslI CCNNNNNNNGG 1 cut(s) 775
BsmAI GTCTC 4 cut(s) 113, 410, 640, 735
BsmFI GGGAC 1 cut(s) 369
BsnI GGCC 1 cut(s) 382
Bso31I GGTCTC 1 cut(s) 113
BsoBI CYCGRG 2 cut(s) 146, 206
Bsp143I GATC 7 cut(s) 36, 264, 292, 394, 401, 457, 660
BspACI CCGC 1 cut(s) 155
BspANI GGCC 1 cut(s) 382
BspCNI CTCAG 3 cut(s) 220, 722, 745
BspHI TCATGA 1 cut(s) 663
BspMI ACCTGC 1 cut(s) 693
BspPI GGATC 1 cut(s) 259
BspQI GCTCTTC 1 cut(s) 153
BspTI CTTAAG 1 cut(s) 296
BspTNI GGTCTC 1 cut(s) 113
BsrI ACTGG 1 cut(s) 812
BssMI GATC 7 cut(s) 36, 264, 292, 394, 401, 457, 660
BssNI GRCGYC 1 cut(s) 742
BssSI CACGAG 1 cut(s) 770
Bst2BI CACGAG 1 cut(s) 770
Bst2UI CCWGG 2 cut(s) 11, 509
Bst4CI ACNGT 2 cut(s) 346, 486
Bst6I CTCTTC 2 cut(s) 153, 448
BstACI GRCGYC 1 cut(s) 742
BstAFI CTTAAG 1 cut(s) 296
BstDEI CTNAG 4 cut(s) 113, 228, 709, 732
BstF5I GGATG 4 cut(s) 53, 157, 627, 784
BstKTI GATC 7 cut(s) 39, 267, 295, 397, 404, 460, 663
BstMAI GTCTC 4 cut(s) 113, 410, 640, 735
BstMBI GATC 7 cut(s) 36, 264, 292, 394, 401, 457, 660
BstMWI GCNNNNNNNGC 3 cut(s) 224, 578, 748
BstNI CCWGG 2 cut(s) 11, 509
BstSCI CCNGG 2 cut(s) 9, 507
BstV1I GCAGC 2 cut(s) 227, 735
BstV2I GAAGAC 2 cut(s) 486, 674
BsuI GTATCC 1 cut(s) 28
BsuRI GGCC 1 cut(s) 382
BtsCI GGATG 4 cut(s) 53, 157, 627, 784
BveI ACCTGC 1 cut(s) 693
CciI TCATGA 1 cut(s) 663
CseI GACGC 1 cut(s) 731
CsiI ACCWGGT 1 cut(s) 9
CviAII CATG 1 cut(s) 664
CviJI RGCY 9 cut(s) 26, 112, 182, 205, 218, 382, 423, 503, 736
CviKI_1 RGCY 9 cut(s) 26, 112, 182, 205, 218, 382, 423, 503, 736
DdeI CTNAG 4 cut(s) 113, 228, 709, 732
DpnI GATC 7 cut(s) 38, 266, 294, 396, 403, 459, 662
DpnII GATC 7 cut(s) 36, 264, 292, 394, 401, 457, 660
Eam1104I CTCTTC 2 cut(s) 153, 448
EarI CTCTTC 2 cut(s) 153, 448
Eco147I AGGCCT 1 cut(s) 382
Eco31I GGTCTC 1 cut(s) 113
Eco57I CTGAAG 1 cut(s) 710
Eco88I CYCGRG 2 cut(s) 146, 206
EcoRII CCWGG 2 cut(s) 9, 507
EcoT22I ATGCAT 1 cut(s) 523
FaeI CATG 1 cut(s) 667
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 1 cut(s) 369
FatI CATG 1 cut(s) 663
Fnu4HI GCNGC 2 cut(s) 216, 749
FokI GGATG 4 cut(s) 40, 164, 634, 791
Fsp4HI GCNGC 2 cut(s) 216, 749
FspBI CTAG 2 cut(s) 270, 420
GluI GCNGC 2 cut(s) 216, 749
GsaI CCCAGC 1 cut(s) 26
GsuI CTGGAG 1 cut(s) 89
HaeIII GGCC 1 cut(s) 382
HgaI GACGC 1 cut(s) 731
Hin1I GRCGYC 1 cut(s) 742
Hin1II CATG 1 cut(s) 667
HinfI GANTC 2 cut(s) 133, 680
HphI GGTGA 1 cut(s) 497
Hpy166II GTNNAC 1 cut(s) 369
Hpy188I TCNGA 2 cut(s) 640, 712
Hpy188III TCNNGA 7 cut(s) 106, 146, 208, 461, 478, 655, 664
Hpy8I GTNNAC 1 cut(s) 369
Hpy99I CGWCG 1 cut(s) 402
HpyAV CCTTC 2 cut(s) 205, 506
HpyCH4III ACNGT 2 cut(s) 346, 486
HpyCH4V TGCA 5 cut(s) 31, 241, 521, 702, 751
HpyF10VI GCNNNNNNNGC 3 cut(s) 224, 578, 748
HpyF3I CTNAG 4 cut(s) 113, 228, 709, 732
Hsp92I GRCGYC 1 cut(s) 742
Hsp92II CATG 1 cut(s) 667
Kzo9I GATC 7 cut(s) 36, 264, 292, 394, 401, 457, 660
LguI GCTCTTC 1 cut(s) 153
LpnPI CCDG 9 cut(s) 23, 36, 72, 119, 314, 494, 521, 688, 793
Lsp1109I GCAGC 2 cut(s) 227, 735
LweI GCATC 7 cut(s) 62, 117, 142, 214, 312, 530, 805
MabI ACCWGGT 1 cut(s) 9
MaeI CTAG 2 cut(s) 270, 420
MaeIII GTNAC 2 cut(s) 438, 685
MalI GATC 7 cut(s) 38, 266, 294, 396, 403, 459, 662
MboI GATC 7 cut(s) 36, 264, 292, 394, 401, 457, 660
MboII GAAGA 7 cut(s) 170, 396, 465, 486, 670, 679, 716
MluCI AATT 5 cut(s) 167, 189, 552, 575, 672
MlyI GAGTC 1 cut(s) 689
MmeI TCCRAC 2 cut(s) 198, 756
MnlI CCTC 8 cut(s) 102, 113, 142, 223, 346, 542, 776, 779
Mph1103I ATGCAT 1 cut(s) 523
MroXI GAANNNNTTC 1 cut(s) 723
MseI TTAA 3 cut(s) 297, 315, 363
MslI CAYNNNNRTG 1 cut(s) 801
MspCI CTTAAG 1 cut(s) 296
MspR9I CCNGG 2 cut(s) 11, 509
MvaI CCWGG 2 cut(s) 11, 509
MwoI GCNNNNNNNGC 3 cut(s) 224, 578, 748
NdeII GATC 7 cut(s) 36, 264, 292, 394, 401, 457, 660
NlaIII CATG 1 cut(s) 667
NmuCI GTSAC 1 cut(s) 438
NsiI ATGCAT 1 cut(s) 523
PaeR7I CTCGAG 2 cut(s) 146, 206
PagI TCATGA 1 cut(s) 663
PceI AGGCCT 1 cut(s) 382
PciSI GCTCTTC 1 cut(s) 153
PdmI GAANNNNTTC 1 cut(s) 723
PfeI GAWTC 1 cut(s) 133
PflFI GACNNNGTC 1 cut(s) 11
PkrI GCNGC 2 cut(s) 217, 750
PleI GAGTC 1 cut(s) 688
PpsI GAGTC 1 cut(s) 688
Psp6I CCWGG 2 cut(s) 9, 507
PspFI CCCAGC 1 cut(s) 22
PspGI CCWGG 2 cut(s) 9, 507
PsyI GACNNNGTC 1 cut(s) 11
RseI CAYNNNNRTG 1 cut(s) 801
SapI GCTCTTC 1 cut(s) 153
SaqAI TTAA 3 cut(s) 297, 315, 363
SatI GCNGC 2 cut(s) 216, 749
Sau3AI GATC 7 cut(s) 36, 264, 292, 394, 401, 457, 660
SchI GAGTC 1 cut(s) 689
ScrFI CCNGG 2 cut(s) 11, 509
SexAI ACCWGGT 1 cut(s) 9
SfaNI GCATC 7 cut(s) 62, 117, 142, 214, 312, 530, 805
Sfr274I CTCGAG 2 cut(s) 146, 206
SlaI CTCGAG 2 cut(s) 146, 206
SmiMI CAYNNNNRTG 1 cut(s) 801
SmlI CTYRAG 4 cut(s) 146, 206, 296, 498
SmoI CTYRAG 4 cut(s) 146, 206, 296, 498
Sse9I AATT 5 cut(s) 167, 189, 552, 575, 672
SseBI AGGCCT 1 cut(s) 382
SsiI CCGC 1 cut(s) 155
SspMI CTAG 2 cut(s) 270, 420
StuI AGGCCT 1 cut(s) 382
StyD4I CCNGG 2 cut(s) 9, 507
TaaI ACNGT 2 cut(s) 346, 486
TaqI TCGA 5 cut(s) 147, 207, 400, 612, 678
TasI AATT 5 cut(s) 167, 189, 552, 575, 672
TfiI GAWTC 1 cut(s) 133
Tru1I TTAA 3 cut(s) 297, 315, 363
Tru9I TTAA 3 cut(s) 297, 315, 363
TseFI GTSAC 1 cut(s) 438
TseI GCWGC 2 cut(s) 215, 748
Tsp45I GTSAC 1 cut(s) 438
TspDTI ATGAA 1 cut(s) 680
TspGWI ACGGA 2 cut(s) 249, 397
Tth111I GACNNNGTC 1 cut(s) 11
Vha464I CTTAAG 1 cut(s) 296
XapI RAATTY 1 cut(s) 189
XhoI CTCGAG 2 cut(s) 146, 206
XmnI GAANNNNTTC 1 cut(s) 723
XspI CTAG 2 cut(s) 270, 420
Zsp2I ATGCAT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.