Prupe.8G077100_v2.0.a1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000400
Physical Location & Seq
Forward (+)
11136571 .. 11141502
4932 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G077100.1

Sequence Viewer

Length: 3528 bp
ATGGAAGCAGCAGGCATAATTTTATCCCCTGCTTTGCAAGTGATCTTTGACAGATTGGCATGCCCAGCCTTAGAAAAGGTTGCTGATATCTGGGGTGTCGAGGACAACCGCGACAGCCTACGAGATGCCTTGATGAGGACTCAAGTTATTCTTCAAGATGCAGAGGAGCAACAATTAACCAACAAATCTGTTAGGCTTTGGTTGTCAAACCTCAAGAATGCTGCTTCTGATGCTGAGGACATTCTGGACTTGTTTATCGCTTCCCAAACCGTCATCCGCTTTAAAAAACCAGGTGCAATTTTTGGTTTTCCAATTACAACAGCCTCTGTAGCTGAAACACTCCACAGATTAGAAAAGAAGATAAATGAGGGACTCTCGACATTTAATTTTAGAGAGCCTAGCTTAGAGCAAGACTGGCTCTTGCGTAGACGACTCAAATTCTTCACTAACAAGAGGGAGACTGGCTCTTGCGTCGTCGACTCAAAGATATATGGAAGAGATGATGAGAAAGAGAAGTTAGTCAAACTGTTACTATCTTCGGAAACATCCCAGGATGAATATGCAACTTGTATACCAGTAATTGGTATTGGAGGAATTGGAAAGACAACTCTTGCTCAATTGGCATATAATGATGAGAGGGTACTCCAACATTTCGACTCTAGAATATGGATTTTTGTTTCTGAGGATTTCAATGTCAAGACGATTATGAAGACAGCCATTGAGTGTGCAACAGAGGATGAATGCAAGTTGTCGGAGATTGAACTACTTCAGTCTCGGCTTTCAAAATTGCTACAGAAGAAAAGATACCTGATTGTGTTAGATGATGTTTGGACTGAAGACCAGGATGATTGGGACAATCTAATACCTTTGTTTACAGGGGGTCTTGATGGATGCAAAATCATTGTCACTACCCGCAGTCAAAAAATTCCATTCATGATGGACTTCCCGAATTCACCATTTTATTTAAATGGTTTGAAGGATCATGATTGCTGGTCTTTGTTCAAGCACCGGGCATTTGGACGAGGAGAAGAAGAGAAGTATCCAAATCTTACACGGATTGGGAAGGAGATTATCAAAAAAATTGGAGGTGTGCCATTAGCTGCAAAAAGATTGGGAAGCTCAATGCGCTTGAAAAGAGAAGAAAAACAGTGGTTATTTATGCGAGATTGTGAACTCTGGGAATTAGATGAAAGCCAACATAAAGTTTTCCCTGCCCTGATGTTGAGTCTATCACCACATCTTAGACAATGCTTTGCATTCTGCTCACTATTTCCCAAAAAATATGAGTTCAATAAGCACAAGTTAATTCATCTATGGATGGCAGAAGGCTTCATTCCAAAAGAGGGAAGCAAGCGACCTGAAGACATTGGTGACGAATACTTTTCCGAATTGTTGTGGATATCTTTCTTGCAAGAAGTACAGCTACACGAAGGTGGAGAAACAATTGGATACAAGATGAATGAGATCATTCATGATTTTGCACGATATGTTGCAGGAAAAGAATATGTGGTACTTGAACAAGGTCGGCCACAAAATTGGTCACCAGCAGAGATTCGCCACGCATCTGTTGTTTATACATACGGTGCAATTACAATTCCAGAAACTCTATATGAAGCAAAACATTTGCGAACTCTCTTATTGATCGGAGATTCCGGCTTCTTAAACATAGATAAAATTTATTCAAGTTTTGAATACTTGCGTGTGCTAGATCTCAACAACTGTGATCTTGTTGATTTGCCTAAATCCTTGAGTGGCTTCATGTGTTTGAGGTATCTTGACTTGTCTTACACGCTTATCTCCCAGTTACCTGAAGGCATGAAGTATCTCTTTTTTTTGCAGACCTTAAACCTACTTGGTTGCCATAATCTTGAGATTTTGCCTTCTTTGGGATTAAACCTAAGACATCTGAACTTAAGTGGATGTGTGCGTTTGACTGGTATGCCTTCTACAATTGGATTGTTAGTTCAACTTCAGACATTGCCACTTTTTGTTGTGGCGAATAAGGAAAGGAATATTCAGCTGCAATATTTAAATCTTCATGGGGAGTTGAATATTACTGGCTTGGAGAATATTGAAGTTGCTTCTTCAGCGGAATTGGCAGAACTGCATATGAAGATAAATCTTGAGTCATTGGGATTATACTGGGGTTTTTTTCTGGGATTTAAAGATTCATTTGCCAAACCACCTAATGCCCCACCCGAAGTAGGTGTCTCAAGATTACATATAGCGCCGCAACCTGAACAGGTTATTGAAAGCCTGCAACCAAGCAAAAATCTGAAAAAGCTAGTTATAAATGGATATCCAGGAACCGAATTTCCTGATTGGGCTCTCCCAAATCTTATTGCCGCTGATTTCACCAACTGTAGAAGTTGTAAACATCTTCCAGCCCTTGGGGAACTTCCGCTACTTAAGACGCTTTCTTTGCATGGAATGCATGGCGTTAAGAGAATTGGTACAGAGTTCTATGGTGACGGTACTGACATATGGTTTCCATCACTCGAAGAACTATCAATAAGTGATTTTGCAAACTTGGAAGAGTGGTCAAGTGCAAATGATGGAAATGCATTCCGTAGACTGAAGAAATTAACTGTAAAGAGTTGCCCGAAGTTAGCACATATACCGCTACCTCAGTCCCTTCAACATTTGGAGCTGCGAGATTGTAATACTGGGATGATGCCCATGGCAGATTTAAGTTTGCTATCTGTGCTTATTCTTGACAAAATTCCAGAATTAGTGTCTCTCCCAGAAGGGCTCTTTGCATCAGCTAGTCTGTCTTCCTTGAAGATTTTGTCTTGCCCCAAGCTTCATTCAATGCCCTTGCATATGCAAAACCTTAGTTCTCTGAAATCATTGACCATTCGTTGGTGTGGAGAGCTGTCCTCTCTGCCACAAAGTTTGCAGAACCTTAAAGCTCTGGAGTCAATGGAGATTAGTGACTGTCACAGTCTAACATCCCTGCCAAATTGTGGAATTGCAGGTTTGGCTTCGCTTCGAACTTTGTCCATTGAAAACTGCAGTAAACTAACTTCTCTGTCGTCTAGTTTGGAACATCTCACATTGCTTGAGCACTTGACTATCATGTACTGTCCAAAACTTGGTTCTTTTCCAGCCGGTGTGCAACACCTCTCCTCCCTTCGAAGTTTGATTGTTCTGAGCTGTCCTTGGTTTGATTCTCTGCCAGAAGGGTTGGAAAATGTAAAGACCCTGCACTGCTTGGAAATTAGTAGCTGCCCCAATTTAACAGCTTTGCCAGAATGGTTTGAGGATCTTGATTCTCTTAGATCTTTGACCATATATGATTGTCCTAATTTAAAAATGCTGTCACCGGGTTTCAAGCTGCTCACTAAACTCCAGCACCTCTCTATTCAAGAATGTCCTGAGCTTGAGGAAAGATGTAGATGCGGTAGTGGTGAGGATTGGTCGAAAATAGCCCATGTCCCGCATAAGTACATTGGATCGCCTCAGGTCAGGCAGTCTGGAGATGCAAGCACGTCTGGCAGCTCTTCTATCCAAGCATCTTCTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1176

Amino Acids

132.37

Weight (kDa)

5.83

Isoelectric Point (pI)

55.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2291
Acc36I ACCTGC 1 cut(s) 2966
AccB7I CCANNNNNTGG 5 cut(s) 581, 2390, 2862, 2966, 3095
AccI GTMKAC 4 cut(s) 427, 477, 571, 2572
AccII CGCG 1 cut(s) 111
AclWI GGATC 3 cut(s) 987, 3273, 3463
AcoI YGGCCR 1 cut(s) 1526
AcsI RAATTY 6 cut(s) 437, 924, 949, 1674, 2312, 2721
AcuI CTGAAG 7 cut(s) 752, 855, 1380, 1830, 1955, 2070, 2597
AfaI GTAC 7 cut(s) 642, 1419, 1512, 2455, 2476, 3083, 3449
AfiI CCNNNNNNNGG 9 cut(s) 135, 581, 1343, 1886, 2204, 2390, 2862, 2966, 3095
AflII CTTAAG 2 cut(s) 1912, 2408
AjiI CACGTC 1 cut(s) 3492
AjnI CCWGG 4 cut(s) 289, 549, 840, 2302
AjuI GAANNNNNNNTTGG 2 cut(s) 3082, 3114
AleI CACNNNNGTG 1 cut(s) 1431
AloI GAACNNNNNNTCC 4 cut(s) 1947, 1979, 2299, 2331
Alw21I GWGCWC 1 cut(s) 3069
Alw26I GTCTC 4 cut(s) 452, 777, 2215, 2742
AlwI GGATC 3 cut(s) 987, 3273, 3463
AlwNI CAGNNNCTG 1 cut(s) 326
AoxI GGCC 1 cut(s) 1526
ApeKI GCWGC 8 cut(s) 8, 221, 1100, 2020, 2650, 3228, 3337, 3498
ApoI RAATTY 6 cut(s) 437, 924, 949, 1674, 2312, 2721
ArsI GACNNNNNNTTYG 6 cut(s) 888, 920, 2193, 2225, 2404, 2436
Asp700I GAANNNNTTC 1 cut(s) 765
AspLEI GCGC 2 cut(s) 1128, 2230
AsuC2I CCSGG 2 cut(s) 1010, 3327
AsuHPI GGTGA 8 cut(s) 945, 1224, 1382, 1533, 2347, 2480, 3315, 3422
AsuII TTCGAA 2 cut(s) 2992, 3136
AxyI CCTNAGG 1 cut(s) 3462
BanII GRGCYC 2 cut(s) 2329, 2754
BarI GAAGNNNNNNTAC 6 cut(s) 788, 820, 1407, 1439, 3010, 3042
BbsI GAAGAC 4 cut(s) 716, 843, 1368, 2766
Bbv12I GWGCWC 1 cut(s) 3069
BbvCI CCTCAGC 1 cut(s) 234
BbvI GCAGC 8 cut(s) 20, 208, 1087, 2007, 2637, 3215, 3324, 3510
BccI CCATC 5 cut(s) 881, 931, 1312, 2500, 2549
BciT130I CCWGG 4 cut(s) 291, 551, 842, 2304
BciVI GTATCC 2 cut(s) 1050, 1442
BcnI CCSGG 2 cut(s) 1010, 3327
BcoDI GTCTC 4 cut(s) 452, 777, 2215, 2742
BfaI CTAG 6 cut(s) 399, 660, 1706, 2285, 2766, 3039
BfmI CTRYAG 4 cut(s) 327, 791, 2362, 3013
BfoI RGCGCY 1 cut(s) 2231
BfrI CTTAAG 2 cut(s) 1912, 2408
BfuAI ACCTGC 1 cut(s) 2966
BfuI GTATCC 2 cut(s) 1050, 1442
BglII AGATCT 2 cut(s) 1708, 3281
Bme1390I CCNGG 6 cut(s) 291, 551, 842, 1010, 2304, 3327
BmgBI CACGTC 1 cut(s) 3492
BmiI GGNNCC 1 cut(s) 2308
BmrFI CCNGG 6 cut(s) 291, 551, 842, 1010, 2304, 3327
BmrI ACTGGG 3 cut(s) 1795, 2152, 2676
BmuI ACTGGG 3 cut(s) 1795, 2152, 2676
BpiI GAAGAC 4 cut(s) 716, 843, 1368, 2766
BplI GAGNNNNNCTC 6 cut(s) 359, 391, 449, 481, 2864, 2896
BpmI CTGGAG 3 cut(s) 2936, 3335, 3498
Bpu10I CCTNAGC 2 cut(s) 234, 3378
Bpu14I TTCGAA 2 cut(s) 2992, 3136
BpuEI CTTGAG 8 cut(s) 126, 197, 1768, 1889, 2144, 2197, 3083, 3404
BpuMI CCSGG 2 cut(s) 1010, 3327
BsaJI CCNNGG 4 cut(s) 549, 2389, 2679, 3161
BsaXI ACNNNNNCTCC 2 cut(s) 3113, 3143
Bsc4I CCNNNNNNNGG 9 cut(s) 135, 581, 1343, 1886, 2204, 2390, 2862, 2966, 3095
Bse118I RCCGGY 1 cut(s) 3110
Bse1I ACTGG 8 cut(s) 419, 466, 575, 1801, 1939, 2062, 2147, 2671
Bse21I CCTNAGG 1 cut(s) 3462
Bse3DI GCAATG 2 cut(s) 1976, 3056
BseBI CCWGG 4 cut(s) 291, 551, 842, 2304
BseDI CCNNGG 4 cut(s) 549, 2389, 2679, 3161
BseLI CCNNNNNNNGG 9 cut(s) 135, 581, 1343, 1886, 2204, 2390, 2862, 2966, 3095
BseMI GCAATG 2 cut(s) 1976, 3056
BseMII CTCAG 6 cut(s) 225, 672, 2642, 3143, 3369, 3476
BseNI ACTGG 8 cut(s) 419, 466, 575, 1801, 1939, 2062, 2147, 2671
BseRI GAGGAG 3 cut(s) 179, 1038, 3118
BseXI GCAGC 8 cut(s) 20, 208, 1087, 2007, 2637, 3215, 3324, 3510
BseYI CCCAGC 1 cut(s) 64
BsgI GTGCAG 1 cut(s) 3191
Bsh1236I CGCG 1 cut(s) 111
BshFI GGCC 1 cut(s) 1528
BsiHKAI GWGCWC 1 cut(s) 3069
BsiSI CCGG 4 cut(s) 1009, 1653, 3111, 3326
BslFI GGGAC 4 cut(s) 384, 866, 2617, 3422
BslI CCNNNNNNNGG 9 cut(s) 135, 581, 1343, 1886, 2204, 2390, 2862, 2966, 3095
BsmAI GTCTC 4 cut(s) 452, 777, 2215, 2742
BsmFI GGGAC 4 cut(s) 384, 866, 2617, 3422
BsmI GAATGC 5 cut(s) 223, 746, 1256, 2436, 2564
BsnI GGCC 1 cut(s) 1528
Bsp119I TTCGAA 2 cut(s) 2992, 3136
Bsp1286I GDGCHC 3 cut(s) 2329, 2754, 3069
Bsp143I GATC 9 cut(s) 42, 979, 1464, 1641, 1708, 1723, 3265, 3281, 3455
Bsp19I CCATGG 1 cut(s) 2679
BspANI GGCC 1 cut(s) 1528
BspCNI CTCAG 6 cut(s) 226, 673, 2641, 3144, 3370, 3475
BspFNI CGCG 1 cut(s) 111
BspHI TCATGA 3 cut(s) 933, 982, 1471
BspLI GGNNCC 1 cut(s) 2308
BspMAI CTGCAG 1 cut(s) 3017
BspMI ACCTGC 1 cut(s) 2966
BspPI GGATC 3 cut(s) 987, 3273, 3463
BspQI GCTCTTC 1 cut(s) 3508
BspT104I TTCGAA 2 cut(s) 2992, 3136
BspTI CTTAAG 2 cut(s) 1912, 2408
BsrDI GCAATG 2 cut(s) 1976, 3056
BsrFI RCCGGY 1 cut(s) 3110
BsrI ACTGG 8 cut(s) 419, 466, 575, 1801, 1939, 2062, 2147, 2671
BssAI RCCGGY 1 cut(s) 3110
BssECI CCNNGG 4 cut(s) 549, 2389, 2679, 3161
BssMI GATC 9 cut(s) 42, 979, 1464, 1641, 1708, 1723, 3265, 3281, 3455
BssNAI GTATAC 1 cut(s) 572
BssT1I CCWWGG 3 cut(s) 2389, 2679, 3161
Bst1107I GTATAC 1 cut(s) 572
Bst2UI CCWGG 4 cut(s) 291, 551, 842, 2304
Bst6I CTCTTC 4 cut(s) 490, 1026, 2529, 3508
BstAFI CTTAAG 2 cut(s) 1912, 2408
BstAPI GCANNNNNTGC 1 cut(s) 2431
BstBI TTCGAA 2 cut(s) 2992, 3136
BstC8I GCNNGC 5 cut(s) 13, 61, 1352, 2258, 3487
BstDSI CCRYGG 1 cut(s) 2679
BstEII GGTNACC 1 cut(s) 1539
BstENI CCTNNNNNAGG 1 cut(s) 133
BstFNI CGCG 1 cut(s) 111
BstH2I RGCGCY 1 cut(s) 2231
BstHHI GCGC 2 cut(s) 1128, 2230
BstKTI GATC 9 cut(s) 45, 982, 1467, 1644, 1711, 1726, 3268, 3284, 3458
BstMAI GTCTC 4 cut(s) 452, 777, 2215, 2742
BstMBI GATC 9 cut(s) 42, 979, 1464, 1641, 1708, 1723, 3265, 3281, 3455
BstNI CCWGG 4 cut(s) 291, 551, 842, 2304
BstNSI RCATGY 1 cut(s) 63
BstPI GGTNACC 1 cut(s) 1539
BstSCI CCNGG 6 cut(s) 289, 549, 840, 1008, 2302, 3325
BstSFI CTRYAG 4 cut(s) 327, 791, 2362, 3013
BstUI CGCG 1 cut(s) 111
BstV1I GCAGC 8 cut(s) 20, 208, 1087, 2007, 2637, 3215, 3324, 3510
BstV2I GAAGAC 4 cut(s) 716, 843, 1368, 2766
BstX2I RGATCY 3 cut(s) 1708, 3265, 3281
BstXI CCANNNNNNTGG 1 cut(s) 1536
BstYI RGATCY 3 cut(s) 1708, 3265, 3281
BstZ17I GTATAC 1 cut(s) 572
Bsu36I CCTNAGG 1 cut(s) 3462
BsuI GTATCC 2 cut(s) 1050, 1442
BsuRI GGCC 1 cut(s) 1528
BtgI CCRYGG 1 cut(s) 2679
BtrI CACGTC 1 cut(s) 3492
BtsI GCAGTG 1 cut(s) 3208
BtsIMutI CAGTG 2 cut(s) 1154, 3208
BveI ACCTGC 1 cut(s) 2966
Cac8I GCNNGC 5 cut(s) 13, 61, 1352, 2258, 3487
CaiI CAGNNNCTG 1 cut(s) 326
CciI TCATGA 3 cut(s) 933, 982, 1471
CfoI GCGC 2 cut(s) 1128, 2230
Cfr10I RCCGGY 1 cut(s) 3110
CseI GACGC 2 cut(s) 460, 2422
CsiI ACCWGGT 1 cut(s) 289
Csp6I GTAC 7 cut(s) 641, 1418, 1511, 2454, 2475, 3082, 3448
CspCI CAANNNNNGTGG 2 cut(s) 2877, 2912
CviQI GTAC 7 cut(s) 641, 1418, 1511, 2454, 2475, 3082, 3448
DpnI GATC 9 cut(s) 44, 981, 1466, 1643, 1710, 1725, 3267, 3283, 3457
DpnII GATC 9 cut(s) 42, 979, 1464, 1641, 1708, 1723, 3265, 3281, 3455
DraI TTTAAA 5 cut(s) 283, 966, 2031, 2164, 3312
EaeI YGGCCR 1 cut(s) 1526
Eam1104I CTCTTC 4 cut(s) 490, 1026, 2529, 3508
EarI CTCTTC 4 cut(s) 490, 1026, 2529, 3508
Eco130I CCWWGG 3 cut(s) 2389, 2679, 3161
Eco24I GRGCYC 2 cut(s) 2329, 2754
Eco32I GATATC 3 cut(s) 88, 1401, 2300
Eco57I CTGAAG 7 cut(s) 752, 855, 1380, 1830, 1955, 2070, 2597
Eco81I CCTNAGG 1 cut(s) 3462
Eco91I GGTNACC 1 cut(s) 1539
EcoNI CCTNNNNNAGG 1 cut(s) 133
EcoO65I GGTNACC 1 cut(s) 1539
EcoRI GAATTC 1 cut(s) 949
EcoRII CCWGG 4 cut(s) 289, 549, 840, 2302
EcoRV GATATC 3 cut(s) 88, 1401, 2300
EcoT14I CCWWGG 3 cut(s) 2389, 2679, 3161
EcoT22I ATGCAT 2 cut(s) 2436, 2566
EcoT38I GRGCYC 2 cut(s) 2329, 2754
ErhI CCWWGG 3 cut(s) 2389, 2679, 3161
FalI AAGNNNNNCTT 4 cut(s) 135, 167, 1811, 1843
FaqI GGGAC 4 cut(s) 384, 866, 2617, 3422
FauI CCCGC 2 cut(s) 920, 3447
FauNDI CATATG 3 cut(s) 2109, 2483, 2823
FblI GTMKAC 4 cut(s) 427, 477, 571, 2572
FriOI GRGCYC 2 cut(s) 2329, 2754
FspBI CTAG 6 cut(s) 399, 660, 1706, 2285, 2766, 3039
GlaI GCGC 2 cut(s) 1127, 2229
GsaI CCCAGC 1 cut(s) 68
GsuI CTGGAG 3 cut(s) 2936, 3335, 3498
HaeII RGCGCY 1 cut(s) 2231
HaeIII GGCC 1 cut(s) 1528
HapII CCGG 4 cut(s) 1009, 1653, 3111, 3326
HgaI GACGC 2 cut(s) 460, 2422
HhaI GCGC 2 cut(s) 1128, 2230
Hin6I GCGC 2 cut(s) 1126, 2228
HinP1I GCGC 2 cut(s) 1126, 2228
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HindIII AAGCTT 1 cut(s) 2801
HpaII CCGG 4 cut(s) 1009, 1653, 3111, 3326
HphI GGTGA 8 cut(s) 945, 1224, 1382, 1533, 2347, 2480, 3315, 3422
Hpy166II GTNNAC 8 cut(s) 428, 478, 572, 873, 1172, 2375, 2573, 3020
Hpy8I GTNNAC 8 cut(s) 428, 478, 572, 873, 1172, 2375, 2573, 3020
Hpy99I CGWCG 2 cut(s) 476, 479
HpyCH4IV ACGT 1 cut(s) 3491
HpySE526I ACGT 1 cut(s) 3491
HspAI GCGC 2 cut(s) 1126, 2228
Kzo9I GATC 9 cut(s) 42, 979, 1464, 1641, 1708, 1723, 3265, 3281, 3455
LguI GCTCTTC 1 cut(s) 3508
LmnI GCTCC 2 cut(s) 166, 2647
Lsp1109I GCAGC 8 cut(s) 20, 208, 1087, 2007, 2637, 3215, 3324, 3510
MabI ACCWGGT 1 cut(s) 289
MaeI CTAG 6 cut(s) 399, 660, 1706, 2285, 2766, 3039
MaeII ACGT 1 cut(s) 3491
MaeIII GTNAC 9 cut(s) 528, 904, 1370, 1539, 1803, 2468, 2933, 2939, 3321
MalI GATC 9 cut(s) 44, 981, 1466, 1643, 1710, 1725, 3267, 3283, 3457
MboI GATC 9 cut(s) 42, 979, 1464, 1641, 1708, 1723, 3265, 3281, 3455
MfeI CAATTG 3 cut(s) 617, 1443, 1950
MflI RGATCY 3 cut(s) 1708, 3265, 3281
MhlI GDGCHC 3 cut(s) 2329, 2754, 3069
MlyI GAGTC 8 cut(s) 133, 366, 426, 473, 650, 1234, 2135, 2927
MmeI TCCRAC 3 cut(s) 670, 732, 3168
Mph1103I ATGCAT 2 cut(s) 2436, 2566
MroXI GAANNNNTTC 1 cut(s) 765
MslI CAYNNNNRTG 1 cut(s) 1431
MspA1I CMGCKG 3 cut(s) 2020, 2090, 2348
MspCI CTTAAG 2 cut(s) 1912, 2408
MspI CCGG 4 cut(s) 1009, 1653, 3111, 3326
MspR9I CCNGG 6 cut(s) 291, 551, 842, 1010, 2304, 3327
MunI CAATTG 3 cut(s) 617, 1443, 1950
Mva1269I GAATGC 5 cut(s) 223, 746, 1256, 2436, 2564
MvaI CCWGG 4 cut(s) 291, 551, 842, 2304
MvnI CGCG 1 cut(s) 111
NciI CCSGG 2 cut(s) 1010, 3327
NcoI CCATGG 1 cut(s) 2679
NdeI CATATG 3 cut(s) 2109, 2483, 2823
NdeII GATC 9 cut(s) 42, 979, 1464, 1641, 1708, 1723, 3265, 3281, 3455
NlaIV GGNNCC 1 cut(s) 2308
NmeAIII GCCGAG 1 cut(s) 754
NmuCI GTSAC 7 cut(s) 904, 1370, 1539, 2468, 2933, 2939, 3321
NsiI ATGCAT 2 cut(s) 2436, 2566
NspI RCATGY 1 cut(s) 63
NspV TTCGAA 2 cut(s) 2992, 3136
OliI CACNNNNGTG 1 cut(s) 1431
PaeI GCATGC 1 cut(s) 63
PagI TCATGA 3 cut(s) 933, 982, 1471
PciSI GCTCTTC 1 cut(s) 3508
PctI GAATGC 5 cut(s) 223, 746, 1256, 2436, 2564
PdmI GAANNNNTTC 1 cut(s) 765
PfeI GAWTC 5 cut(s) 1552, 1649, 2168, 3170, 3272
PflMI CCANNNNNTGG 5 cut(s) 581, 2390, 2862, 2966, 3095
PfoI TCCNGGA 1 cut(s) 2302
PleI GAGTC 8 cut(s) 133, 366, 426, 473, 650, 1233, 2134, 2926
PpsI GAGTC 8 cut(s) 133, 366, 426, 473, 650, 1233, 2134, 2926
PsiI TTATAA 1 cut(s) 2291
Psp6I CCWGG 4 cut(s) 289, 549, 840, 2302
PspEI GGTNACC 1 cut(s) 1539
PspFI CCCAGC 1 cut(s) 64
PspGI CCWGG 4 cut(s) 289, 549, 840, 2302
PspN4I GGNNCC 1 cut(s) 2308
PsrI GAACNNNNNNTAC 2 cut(s) 2388, 2420
PstI CTGCAG 1 cut(s) 3017
PstNI CAGNNNCTG 1 cut(s) 326
PsuI RGATCY 3 cut(s) 1708, 3265, 3281
PvuII CAGCTG 1 cut(s) 2020
RsaI GTAC 7 cut(s) 642, 1419, 1512, 2455, 2476, 3083, 3449
RsaNI GTAC 7 cut(s) 641, 1418, 1511, 2454, 2475, 3082, 3448
RseI CAYNNNNRTG 1 cut(s) 1431
SalI GTCGAC 1 cut(s) 476
SapI GCTCTTC 1 cut(s) 3508
Sau3AI GATC 9 cut(s) 42, 979, 1464, 1641, 1708, 1723, 3265, 3281, 3455
SchI GAGTC 8 cut(s) 133, 366, 426, 473, 650, 1234, 2135, 2927
ScrFI CCNGG 6 cut(s) 291, 551, 842, 1010, 2304, 3327
SduI GDGCHC 3 cut(s) 2329, 2754, 3069
SexAI ACCWGGT 1 cut(s) 289
SfcI CTRYAG 4 cut(s) 327, 791, 2362, 3013
SfuI TTCGAA 2 cut(s) 2992, 3136
SmiI ATTTAAAT 2 cut(s) 966, 2031
SmiMI CAYNNNNRTG 1 cut(s) 1431
SphI GCATGC 1 cut(s) 63
SspI AATATT 4 cut(s) 2014, 2027, 2053, 2071
SspMI CTAG 6 cut(s) 399, 660, 1706, 2285, 2766, 3039
StyD4I CCNGG 6 cut(s) 289, 549, 840, 1008, 2302, 3325
StyI CCWWGG 3 cut(s) 2389, 2679, 3161
SwaI ATTTAAAT 2 cut(s) 966, 2031
TaiI ACGT 1 cut(s) 3494
TaqI TCGA 8 cut(s) 99, 377, 477, 654, 2499, 2992, 3136, 3422
TatI WGTACW 3 cut(s) 1417, 3081, 3447
TauI GCSGC 2 cut(s) 2233, 2348
TfiI GAWTC 5 cut(s) 1552, 1649, 2168, 3170, 3272
TscAI CASTG 2 cut(s) 1154, 3215
TseFI GTSAC 7 cut(s) 904, 1370, 1539, 2468, 2933, 2939, 3321
TseI GCWGC 8 cut(s) 8, 221, 1100, 2020, 2650, 3228, 3337, 3498
Tsp45I GTSAC 7 cut(s) 904, 1370, 1539, 2468, 2933, 2939, 3321
TspGWI ACGGA 2 cut(s) 1069, 2558
TspRI CASTG 2 cut(s) 1154, 3215
Van91I CCANNNNNTGG 5 cut(s) 581, 2390, 2862, 2966, 3095
Vha464I CTTAAG 2 cut(s) 1912, 2408
XagI CCTNNNNNAGG 1 cut(s) 133
XapI RAATTY 6 cut(s) 437, 924, 949, 1674, 2312, 2721
XbaI TCTAGA 1 cut(s) 659
XceI RCATGY 1 cut(s) 63
XmiI GTMKAC 4 cut(s) 427, 477, 571, 2572
XmnI GAANNNNTTC 1 cut(s) 765
XspI CTAG 6 cut(s) 399, 660, 1706, 2285, 2766, 3039
Zsp2I ATGCAT 2 cut(s) 2436, 2566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.