Rroxscaffold_3G00227820

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000400
Physical Location & Seq
Forward (+)
12100387 .. 12103827
3441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00227820.1

Sequence Viewer

Length: 2349 bp
ATGAGCATGGAGCTGAGGATTAATAACTTGCTGAGAAGATGGGAAGATTTAGTAAATGGTCACATGGGAGAGAACAAAATCGAAAGGCTTCAGTTTGTGCTTTTTTACGAACTTTGTGGGCTTTATCCTTCACGTCCGAACATGAAGGATAGGATTGGTATGATGGTGAGAAAGATTGGAGAGCGGATAAAATATGAGGTAAGGAAAACTAGCTTAACTCTTGAATCAATTTCTACTTACCGTAAGAAGTCACGCAAACTAAAGGAGCTTCGTGCAAGATTGGATGATGTAGCCAAGGAAATGTCTTCTTTCCGGTTCAAAGAAACTCAGTCCTATGGGCGGTCAAGTACCATGGAAAGACGTGAAACTGGACCCTCTATCAATGAGTCAAATGTTTATGGCAGAGAAGAGGATGTGGATAAGATTGTGGGGATGTTATTGTCTTCCAGTAGTGGTCCTGAAGTTGCAGTAATTCCCATTGCTGGAATTGGAGGGATGGGTAAAACAACACTCGCCCAGTTAGTCTATAACGATCCGAGGGTTACAAGCCAATTTCAATATTTGATGTGGGTTTCTGTCAATGATAATTTTAACCCGGCAAGGATCATAAATCAAATGTTGAGTTATGTCAGAAAGGGCAGCCATGACTCGTTTCAGATTGGGGTGCTGCAATCTCAATTGAGAGAATCCTTACTGGGAAAAAGATACTTAATAGTGCTCGATGATGTGTGGAATGAGGATCCAGATGAATGGGATAAAGTAATGAATCCATTGAAATGCTCCGCTGATGGAAGCAAAATTATATTAACCACCCGGAGTGAGGCGGTTGCAGCCATAACTAGCACATTTCCCCCTTTTTATTTGGAACCACTAAGAAAAGCAGAGTGCTGGAAGTTATTCAAGCACCGTGCCTTTGCAAATGGAACAGAGGATGGTTTTCCAAGCCTACTGCATATCGGTGAACAAATAGTAGACAAGTGCAAAGGTGTCCCATTGGTTGCAAATGTTCTTGGAAGCCTGCTGCGCTTTAAAAGAGAAGAAAGTGAGTGGTTGCATGTGCAAAGGAGTGAACTATGGAGTATTGATGCAGGCGAGAACAGAATTCTATCCATCTTAAGGCTGAGTTACAATCATTTGCCATCGCATCTGAAAGCTTGCTTTGCATATTGTTCAGTGTTCCCAAAGAATTATGAGATTAATAAGGAAAAGTTGATCCATCAATGGCTCGCACATGGCTTAATTCCTTGTATTGGAGATCCTTCATTTAGGTCGGAGGACATTGGCAATGAGTATTTTAACAATTTGTTGATGATGTCTTTCTTTCAAGAAATAAGAAAATCTGATGACAGAGGTATGTCAGAATTTAATATGCATGATCATATTAATGATCTTGCAAAATCTGTTGCTGGAGAGGAATCATTGACTCTAGGACAGGAAAATGTGCACTGTAGTCTCTCAAAGACATGTCATGCATCAGTTGTTTCCAGTTCTAACTCTGCTTTGATCCCTGAAGCCTTGTGTGAAGCAAAGAGATTGCGAACCCTCAATTTCCTGTCGCCAAGAGAGGATTATTTGGAAGCCATTCCAACCATACTAGCAACTTTTAAACATCTCAGAATGCTGAATTTCAGTGGATCTGGAATTAAGAGTCTCCACCAAGAGATTGGTGGGCTAATATCCTTGCGATATCTTGATCTATCAAATACTCTCCTAGAGACGATGCCAGCGACTATCAGTGATCTGTGCAATTTGCAGACCCTGAATCTCTCAAGTTGTAATGAGCTCAAGGAGTTACCAAGTGGTACTTCCAAGTTAAAAAATCTCAGACATCTTGACATAGATGATTGTCCAAGACTAGCTAGCATGCCCCCATCAATGGGAACTTTACAACAACTCCAAACTTTGCCGGTATATATCGTCGGCTGCAACTTTGAAACTTCTATTTTTCAGCTATCATCAATGGAAAATCTACGAGGGAAGTTAAAAATCAAATGTATGGAGGAGGCTATAATTCCATCTGGGAACAACATGATTAAAGTATGGATGAAAACAAAAGAGTTTCATTCATTGGAACTGTTGTGGCAAAATGATGGGGGTAAGCTAGATCATAATAGATCTAGGCAAGCTGGTAGGCAAGTTGATGATAGTACTGATTTTAGTCTGGTAGATTCTTTGACTGTGTCGCCCTTTATAAGAATGCTGTCTATAAATGGTTATTCAGGAACCAAGTTCCCAGATTGGATGAGTTGGTCTCAGAACTTGACAGAGCTAAACATAATTAATTGCAGAAGATGTGAAAGTCTTCCTCCACTCGGTCAACTTCCTGTCCTCAAGATCCTCAACATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

782

Amino Acids

88.91

Weight (kDa)

7.8

Isoelectric Point (pI)

48.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 136 - 307 9.6e-38 NB-ARC domain
WHD_DRP PF23559 392 - 465 1e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 513 - 782 3.7e-27 Leucine-rich repeat region
LRR_8 PF13855 537 - 593 9.3e-07 Leucine rich repeat
LRR_4 PF12799 583 - 622 7e-06 Leucine Rich repeats (2 copies)
LRR_R13L1-DRL21 PF25019 650 - 782 6e-23 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2192
AccBSI CCGCTC 1 cut(s) 184
AccI GTMKAC 1 cut(s) 972
AciI CCGC 4 cut(s) 184, 340, 783, 824
AclWI GGATC 9 cut(s) 527, 611, 734, 747, 1207, 1250, 1498, 1642, 2329
AcsI RAATTY 3 cut(s) 1101, 1361, 1624
AcuI CTGAAG 3 cut(s) 74, 480, 1530
AfaI GTAC 3 cut(s) 349, 1804, 2149
AfiI CCNNNNNNNGG 8 cut(s) 339, 482, 820, 1116, 1250, 1876, 1877, 2312
AflII CTTAAG 1 cut(s) 1114
AflIII ACRYGT 1 cut(s) 1463
AgsI TTSAA 7 cut(s) 224, 319, 557, 775, 901, 1325, 1934
AjiI CACGTC 2 cut(s) 134, 362
AjuI GAANNNNNNNTTGG 2 cut(s) 1789, 1821
Alw21I GWGCWC 3 cut(s) 720, 1446, 1785
Alw26I GTCTC 4 cut(s) 1457, 1655, 1709, 2256
Alw44I GTGCAC 1 cut(s) 1442
AlwI GGATC 9 cut(s) 527, 611, 734, 747, 1207, 1250, 1498, 1642, 2329
AlwNI CAGNNNCTG 1 cut(s) 1759
ApaLI GTGCAC 1 cut(s) 1442
ApeKI GCWGC 5 cut(s) 639, 667, 830, 1021, 1923
ApoI RAATTY 3 cut(s) 1101, 1361, 1624
AseI ATTAAT 4 cut(s) 21, 1197, 1383, 2280
Asp700I GAANNNNTTC 4 cut(s) 87, 896, 1581, 2301
AspLEI GCGC 1 cut(s) 1026
AspS9I GGNCC 2 cut(s) 371, 455
AsuC2I CCSGG 2 cut(s) 596, 814
AsuHPI GGTGA 2 cut(s) 178, 971
AsuNHI GCTAGC 1 cut(s) 1859
AvaII GGWCC 2 cut(s) 371, 455
BaeGI GKGCMC 1 cut(s) 1446
BamHI GGATCC 1 cut(s) 739
BanII GRGCYC 1 cut(s) 1785
BbsI GAAGAC 3 cut(s) 297, 435, 2294
Bbv12I GWGCWC 3 cut(s) 720, 1446, 1785
BbvCI CCTCAGC 1 cut(s) 14
BbvI GCAGC 5 cut(s) 651, 654, 842, 1008, 1910
BclI TGATCA 1 cut(s) 1375
BcnI CCSGG 2 cut(s) 596, 814
BcoDI GTCTC 4 cut(s) 1457, 1655, 1709, 2256
BfaI CTAG 9 cut(s) 210, 840, 1427, 1595, 1712, 1856, 1860, 2102, 2118
BfmI CTRYAG 1 cut(s) 1447
BfrI CTTAAG 1 cut(s) 1114
BglII AGATCT 1 cut(s) 2114
BisI GCNGC 5 cut(s) 640, 668, 831, 1022, 1924
BlsI GCNGC 5 cut(s) 641, 669, 832, 1023, 1925
BmcAI AGTACT 1 cut(s) 2149
Bme1390I CCNGG 2 cut(s) 596, 814
Bme18I GGWCC 2 cut(s) 371, 455
BmgBI CACGTC 2 cut(s) 134, 362
BmgT120I GGNCC 2 cut(s) 371, 455
BmiI GGNNCC 4 cut(s) 373, 741, 867, 2224
BmrFI CCNGG 2 cut(s) 596, 814
BmrI ACTGGG 2 cut(s) 511, 704
BmsI GCATC 4 cut(s) 1075, 1153, 1481, 1710
BmtI GCTAGC 1 cut(s) 1863
BmuI ACTGGG 2 cut(s) 511, 704
BpiI GAAGAC 3 cut(s) 297, 435, 2294
BplI GAGNNNNNCTC 2 cut(s) 2236, 2268
BpmI CTGGAG 1 cut(s) 1428
Bpu10I CCTNAGC 1 cut(s) 14
BpuEI CTTGAG 3 cut(s) 1753, 1769, 2315
BpuMI CCSGG 2 cut(s) 596, 814
BsaI GGTCTC 1 cut(s) 2256
BsaJI CCNNGG 3 cut(s) 294, 351, 536
BsaWI WCCGGW 1 cut(s) 312
BsaXI ACNNNNNCTCC 2 cut(s) 1878, 1908
Bsc4I CCNNNNNNNGG 8 cut(s) 339, 482, 820, 1116, 1250, 1876, 1877, 2312
Bse118I RCCGGY 1 cut(s) 1906
Bse1I ACTGG 5 cut(s) 373, 447, 517, 699, 1485
Bse3DI GCAATG 2 cut(s) 477, 1291
BseDI CCNNGG 3 cut(s) 294, 351, 536
BseGI GGATG 7 cut(s) 289, 418, 438, 501, 937, 2049, 2247
BseLI CCNNNNNNNGG 8 cut(s) 339, 482, 820, 1116, 1250, 1876, 1877, 2312
BseMI GCAATG 2 cut(s) 477, 1291
BseMII CTCAG 7 cut(s) 5, 23, 341, 1112, 1627, 1837, 2267
BseNI ACTGG 5 cut(s) 373, 447, 517, 699, 1485
BseRI GAGGAG 1 cut(s) 2015
BseSI GKGCMC 1 cut(s) 1446
BseXI GCAGC 5 cut(s) 651, 654, 842, 1008, 1910
BsiHKAI GWGCWC 3 cut(s) 720, 1446, 1785
BsiSI CCGG 4 cut(s) 313, 596, 814, 1907
BslFI GGGAC 1 cut(s) 974
BslI CCNNNNNNNGG 8 cut(s) 339, 482, 820, 1116, 1250, 1876, 1877, 2312
BsmAI GTCTC 4 cut(s) 1457, 1655, 1709, 2256
BsmBI CGTCTC 1 cut(s) 1709
BsmFI GGGAC 1 cut(s) 974
BsmI GAATGC 2 cut(s) 1623, 2202
Bso31I GGTCTC 1 cut(s) 2256
Bsp1286I GDGCHC 3 cut(s) 720, 1446, 1785
Bsp19I CCATGG 1 cut(s) 351
BspACI CCGC 4 cut(s) 184, 340, 783, 824
BspCNI CTCAG 7 cut(s) 6, 24, 340, 1113, 1626, 1836, 2266
BspLI GGNNCC 4 cut(s) 373, 741, 867, 2224
BspOI GCTAGC 1 cut(s) 1863
BspPI GGATC 9 cut(s) 527, 611, 734, 747, 1207, 1250, 1498, 1642, 2329
BspTI CTTAAG 1 cut(s) 1114
BspTNI GGTCTC 1 cut(s) 2256
BsrBI CCGCTC 1 cut(s) 184
BsrDI GCAATG 2 cut(s) 477, 1291
BsrFI RCCGGY 1 cut(s) 1906
BsrI ACTGG 5 cut(s) 373, 447, 517, 699, 1485
BssAI RCCGGY 1 cut(s) 1906
BssECI CCNNGG 3 cut(s) 294, 351, 536
BssT1I CCWWGG 2 cut(s) 294, 351
Bst4CI ACNGT 5 cut(s) 242, 908, 1448, 2076, 2179
Bst6I CTCTTC 1 cut(s) 402
BstAFI CTTAAG 1 cut(s) 1114
BstC8I GCNNGC 8 cut(s) 1019, 1090, 1156, 1227, 1725, 1861, 1865, 2124
BstDEI CTNAG 8 cut(s) 14, 32, 327, 872, 1121, 1613, 1823, 2253
BstDSI CCRYGG 1 cut(s) 351
BstF5I GGATG 7 cut(s) 289, 418, 438, 501, 937, 2049, 2247
BstHHI GCGC 1 cut(s) 1026
BstMAI GTCTC 4 cut(s) 1457, 1655, 1709, 2256
BstMWI GCNNNNNNNGC 3 cut(s) 830, 1023, 1160
BstNSI RCATGY 3 cut(s) 1058, 1467, 1867
BstSCI CCNGG 2 cut(s) 594, 812
BstSFI CTRYAG 1 cut(s) 1447
BstSLI GKGCMC 1 cut(s) 1446
BstV1I GCAGC 5 cut(s) 651, 654, 842, 1008, 1910
BstV2I GAAGAC 3 cut(s) 297, 435, 2294
BstX2I RGATCY 5 cut(s) 739, 1255, 1634, 2114, 2334
BstXI CCANNNNNNTGG 2 cut(s) 750, 1664
BstYI RGATCY 5 cut(s) 739, 1255, 1634, 2114, 2334
BtgI CCRYGG 1 cut(s) 351
BtgZI GCGATG 1 cut(s) 1125
BtrI CACGTC 2 cut(s) 134, 362
BtsCI GGATG 7 cut(s) 289, 418, 438, 501, 937, 2049, 2247
BtsIMutI CAGTG 4 cut(s) 1179, 1444, 1636, 1741
Cac8I GCNNGC 8 cut(s) 1019, 1090, 1156, 1227, 1725, 1861, 1865, 2124
CaiI CAGNNNCTG 1 cut(s) 1759
CfoI GCGC 1 cut(s) 1026
Cfr10I RCCGGY 1 cut(s) 1906
Cfr13I GGNCC 2 cut(s) 371, 455
Csp6I GTAC 3 cut(s) 348, 1803, 2148
CviQI GTAC 3 cut(s) 348, 1803, 2148
DdeI CTNAG 8 cut(s) 14, 32, 327, 872, 1121, 1613, 1823, 2253
DraI TTTAAA 2 cut(s) 1030, 1606
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
Ecl136II GAGCTC 1 cut(s) 1783
Eco130I CCWWGG 2 cut(s) 294, 351
Eco24I GRGCYC 1 cut(s) 1785
Eco31I GGTCTC 1 cut(s) 2256
Eco32I GATATC 1 cut(s) 1688
Eco47I GGWCC 2 cut(s) 371, 455
Eco53kI GAGCTC 1 cut(s) 1783
Eco57I CTGAAG 3 cut(s) 74, 480, 1530
EcoICRI GAGCTC 1 cut(s) 1783
EcoRI GAATTC 1 cut(s) 1101
EcoRV GATATC 1 cut(s) 1688
EcoT14I CCWWGG 2 cut(s) 294, 351
EcoT22I ATGCAT 2 cut(s) 1374, 1474
EcoT38I GRGCYC 1 cut(s) 1785
ErhI CCWWGG 2 cut(s) 294, 351
Esp3I CGTCTC 1 cut(s) 1709
FalI AAGNNNNNCTT 2 cut(s) 1789, 1821
FaqI GGGAC 1 cut(s) 974
FbaI TGATCA 1 cut(s) 1375
FblI GTMKAC 1 cut(s) 972
Fnu4HI GCNGC 5 cut(s) 640, 668, 831, 1022, 1924
FokI GGATG 7 cut(s) 296, 425, 445, 508, 944, 2056, 2254
FriOI GRGCYC 1 cut(s) 1785
Fsp4HI GCNGC 5 cut(s) 640, 668, 831, 1022, 1924
FspBI CTAG 9 cut(s) 210, 840, 1427, 1595, 1712, 1856, 1860, 2102, 2118
GlaI GCGC 1 cut(s) 1025
GluI GCNGC 5 cut(s) 640, 668, 831, 1022, 1924
GsuI CTGGAG 1 cut(s) 1428
HapII CCGG 4 cut(s) 313, 596, 814, 1907
HhaI GCGC 1 cut(s) 1026
Hin6I GCGC 1 cut(s) 1024
HinP1I GCGC 1 cut(s) 1024
HincII GTYRAC 1 cut(s) 2318
HindII GTYRAC 1 cut(s) 2318
HindIII AAGCTT 1 cut(s) 1152
HpaII CCGG 4 cut(s) 313, 596, 814, 1907
HphI GGTGA 2 cut(s) 178, 971
Hpy166II GTNNAC 5 cut(s) 962, 973, 1070, 1444, 2318
Hpy188III TCNNGA 9 cut(s) 221, 458, 743, 1325, 1638, 1691, 1832, 2220, 2332
Hpy8I GTNNAC 5 cut(s) 962, 973, 1070, 1444, 2318
Hpy99I CGWCG 1 cut(s) 1922
HpyAV CCTTC 3 cut(s) 138, 139, 1269
HpyCH4III ACNGT 5 cut(s) 242, 908, 1448, 2076, 2179
HpyCH4IV ACGT 2 cut(s) 133, 361
HpyF10VI GCNNNNNNNGC 3 cut(s) 830, 1023, 1160
HpyF3I CTNAG 8 cut(s) 14, 32, 327, 872, 1121, 1613, 1823, 2253
HpySE526I ACGT 2 cut(s) 133, 361
HspAI GCGC 1 cut(s) 1024
Ksp22I TGATCA 1 cut(s) 1375
LmnI GCTCC 3 cut(s) 10, 265, 785
Lsp1109I GCAGC 5 cut(s) 651, 654, 842, 1008, 1910
LweI GCATC 4 cut(s) 1075, 1153, 1481, 1710
MaeI CTAG 9 cut(s) 210, 840, 1427, 1595, 1712, 1856, 1860, 2102, 2118
MaeII ACGT 2 cut(s) 133, 361
MaeIII GTNAC 5 cut(s) 59, 249, 541, 1124, 1791
MbiI CCGCTC 1 cut(s) 184
MboII GAAGA 8 cut(s) 48, 56, 297, 419, 435, 1049, 2294, 2301
MfeI CAATTG 1 cut(s) 677
MflI RGATCY 5 cut(s) 739, 1255, 1634, 2114, 2334
MhlI GDGCHC 3 cut(s) 720, 1446, 1785
MlyI GAGTC 4 cut(s) 395, 641, 1417, 1657
MmeI TCCRAC 2 cut(s) 1251, 1610
Mph1103I ATGCAT 2 cut(s) 1374, 1474
MroXI GAANNNNTTC 4 cut(s) 87, 896, 1581, 2301
MslI CAYNNNNRTG 3 cut(s) 775, 957, 1383
MspA1I CMGCKG 1 cut(s) 785
MspCI CTTAAG 1 cut(s) 1114
MspI CCGG 4 cut(s) 313, 596, 814, 1907
MspR9I CCNGG 2 cut(s) 596, 814
MunI CAATTG 1 cut(s) 677
Mva1269I GAATGC 2 cut(s) 1623, 2202
MwoI GCNNNNNNNGC 3 cut(s) 830, 1023, 1160
NciI CCSGG 2 cut(s) 596, 814
NcoI CCATGG 1 cut(s) 351
NheI GCTAGC 1 cut(s) 1859
NlaIV GGNNCC 4 cut(s) 373, 741, 867, 2224
NmuCI GTSAC 2 cut(s) 59, 249
NsiI ATGCAT 2 cut(s) 1374, 1474
NspI RCATGY 3 cut(s) 1058, 1467, 1867
PaeI GCATGC 1 cut(s) 1867
PciI ACATGT 1 cut(s) 1463
PcsI WCGNNNNNNNCGW 1 cut(s) 1724
PctI GAATGC 2 cut(s) 1623, 2202
PdmI GAANNNNTTC 4 cut(s) 87, 896, 1581, 2301
PfeI GAWTC 6 cut(s) 224, 686, 766, 1415, 1762, 2168
PflFI GACNNNGTC 1 cut(s) 2179
PkrI GCNGC 5 cut(s) 641, 669, 832, 1023, 1925
PleI GAGTC 4 cut(s) 394, 641, 1417, 1656
PpsI GAGTC 4 cut(s) 394, 641, 1417, 1656
PscI ACATGT 1 cut(s) 1463
PshBI ATTAAT 4 cut(s) 21, 1197, 1383, 2280
PsiI TTATAA 1 cut(s) 2192
Psp124BI GAGCTC 1 cut(s) 1785
PspN4I GGNNCC 4 cut(s) 373, 741, 867, 2224
PspPI GGNCC 2 cut(s) 371, 455
PstNI CAGNNNCTG 1 cut(s) 1759
PsuI RGATCY 5 cut(s) 739, 1255, 1634, 2114, 2334
PsyI GACNNNGTC 1 cut(s) 2179
RsaI GTAC 3 cut(s) 349, 1804, 2149
RsaNI GTAC 3 cut(s) 348, 1803, 2148
RseI CAYNNNNRTG 3 cut(s) 775, 957, 1383
SacI GAGCTC 1 cut(s) 1785
SatI GCNGC 5 cut(s) 640, 668, 831, 1022, 1924
Sau96I GGNCC 2 cut(s) 371, 455
ScaI AGTACT 1 cut(s) 2149
SchI GAGTC 4 cut(s) 395, 641, 1417, 1657
ScrFI CCNGG 2 cut(s) 596, 814
SduI GDGCHC 3 cut(s) 720, 1446, 1785
SfaNI GCATC 4 cut(s) 1075, 1153, 1481, 1710
SfcI CTRYAG 1 cut(s) 1447
SinI GGWCC 2 cut(s) 371, 455
SmiMI CAYNNNNRTG 3 cut(s) 775, 957, 1383
SmlI CTYRAG 4 cut(s) 1114, 1768, 1784, 2330
SmoI CTYRAG 4 cut(s) 1114, 1768, 1784, 2330
SphI GCATGC 1 cut(s) 1867
SsiI CCGC 4 cut(s) 184, 340, 783, 824
SspI AATATT 1 cut(s) 560
SspMI CTAG 9 cut(s) 210, 840, 1427, 1595, 1712, 1856, 1860, 2102, 2118
SstI GAGCTC 1 cut(s) 1785
StyD4I CCNGG 2 cut(s) 594, 812
StyI CCWWGG 2 cut(s) 294, 351
TaaI ACNGT 5 cut(s) 242, 908, 1448, 2076, 2179
TaiI ACGT 2 cut(s) 136, 364
TaqI TCGA 2 cut(s) 81, 720
TaqII GACCGA 1 cut(s) 2303
TatI WGTACW 1 cut(s) 2147
TfiI GAWTC 6 cut(s) 224, 686, 766, 1415, 1762, 2168
TscAI CASTG 4 cut(s) 1179, 1451, 1636, 1741
TseFI GTSAC 2 cut(s) 59, 249
TseI GCWGC 5 cut(s) 639, 667, 830, 1021, 1923
Tsp45I GTSAC 2 cut(s) 59, 249
TspDTI ATGAA 7 cut(s) 158, 762, 779, 1251, 2051, 2055, 2060
TspRI CASTG 4 cut(s) 1179, 1451, 1636, 1741
Tth111I GACNNNGTC 1 cut(s) 2179
Vha464I CTTAAG 1 cut(s) 1114
VneI GTGCAC 1 cut(s) 1442
VpaK11BI GGWCC 2 cut(s) 371, 455
VspI ATTAAT 4 cut(s) 21, 1197, 1383, 2280
XapI RAATTY 3 cut(s) 1101, 1361, 1624
XceI RCATGY 3 cut(s) 1058, 1467, 1867
XcmI CCANNNNNNNNNTGG 2 cut(s) 1661, 1664
XmiI GTMKAC 1 cut(s) 972
XmnI GAANNNNTTC 4 cut(s) 87, 896, 1581, 2301
XspI CTAG 9 cut(s) 210, 840, 1427, 1595, 1712, 1856, 1860, 2102, 2118
ZrmI AGTACT 1 cut(s) 2149
Zsp2I ATGCAT 2 cut(s) 1374, 1474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.