RchiOBHm_Chr7g0232931

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000400
Physical Location & Seq
Reverse (-)
57128076 .. 57131496
3421 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20877

Sequence Viewer

Length: 2664 bp
ATGGAGTCTTTGATTGTATCCCCGCTGCTGCAGCTGCTCTTTAGAAGGTTGACTGACACTGTTGTTGTTGGTCAAGTGCGAACTCGCAGAGAGTTCAGGACGGAGATCGAGAAGATGCAGGAGACGTTACCGATGATTAAGGCGGTGATAGAAGATGCAGAGGAGCAGCAGAGGAAAAATCAGAGGGTGAAGGCTTGGCTGGTAAAGCTCAAAGACGTCGCAGTCGATGCTGATAATCTGCTTGATGAGGTTGCCACCCTAGTTCTGCGCAAGCATTTGATGAAAGAGGAATTTTGCCGTTTATATGCTTCCGACCGATATCGCTCTCGACATCCATCTTCGTGCTCCAGAATACTGTATGATTGCCAACATCGTGAATTTCTTTCTTCACGTACGAATCTGAACAGCAAGGACAGGATTGGCATGATGGTGAGAAAGATTGGAAAGCGGACAAGATATGAGGTAAGGAAAACTTGCTTAACTCTTGAATTGATTTCTACTTACCGTAAGAAGTCACGCAAACTAAAGGAGCTTCGTGCAAGATTGGATGATGTAGCCAAGGAGATGTCTAGTTTCCACTTCAAAGAAACTCAGTCTTATGGACGGTCAAGTACAATGGAGAGACGCCAAACTGGACCCTCTGTCAATGATTCAAAGGTTTATGGCAGAGAAGAGGATGTGGATAAAATTGTGGGGATGCTATTGTCTTCCAGTAGTGGTCCTGAAGTTGCAGTAATTCCCATTGTTGGACTTGCAGGGATGGGGAAAACAACACTTGCTCAGTTAGTCTACAATGATCTGAGGGTTACAAGCCACTTTCAATCTTCAATATGGGTGTCTGTCAATGATAATTTTAACCCGGCAAGGATCATAAATCAAATGTTGAGTTATGTCGGAAAGGGCTGCCATGACTCGTTTCAGATTGGGGTACTGCAATCTCAATTGAGAGAATCGTTACTGGGAAAAAGGTACTTGATAGTGCTCGATGATGTGTGGAATGAGGATCCAGATGAATGGGATAAAGTAATGAATCCATTGAAAGGCTCCCCAGGTGGAAGTAAAATTATACTAACCACCCGGAATAAGCCAGTTGCAGCCATAACTAGCACATTTCCCCCATTTCATTTGGAACCACTGAGAAAAGAAGAGTGCTGGAAGTTGTTCAAGCACCGAGCCTTTGCAGATGGAACAGAGGGTGATTTTCCGAGACTACTGCAGATTGGTGAGAAAATAGTAGACAAGTGCAAAGGTGTCCCGTTGGTTGCAAATATTCTTGGAATCATGCTGCGCTTTAAAAGAGAAGAAAGCGAGTGGTTACATGTGCAAAGGAGTGAACTATGGAGTATTGATGCAGGTGAGAACAGAATTCTATCCATCTTCAGGCTGAGTTACAATCATTTGCCATCGCATCTGAAAGCTTGCTTTGCATATTGTTCAATATTCCCAAAGAATTATGAGATTAATAAGGAAATGTTGATCCATCAATGGCTCGCACATGGCTTAATTCCTCATATTGTGGAGTCTTCAATTAGGCCAGAGGACACTGGCAATGAGTATTTTAACAATTTGTTGATGATGTTCTTCTTCCAAGAAATAAAAAAATATGATGGCAGAGGTATGACAGAATTTAAAATGCATGATCATATTAATGATCTTGCAAAATCTGTTGCTGGAGAGGAATCCTTGACTCTAGAACAGGAAAATGTGCACTGTAGTCTCTCAAAGACATGTCATATATCAGTTGTTTGCAGTTCTAGCTCTGCTTTGATCCCTGAAGCCTTGTGTAAAGCAAAGAGATTGCGAACCCTTAATTTCCTTTCACCAAGAGAGGATTATGTGGAAGCCATTCCAACCATACAAGCAACTTTTAAACACCTCAGAATGCTGAATTTCAGTGGATTTGGAATTAAGAGGCTTCACCCAGAGATTGGTGGGCTACTATCCTTGCGGTATCTTGATCTTTCAAATACTCTCCTAGAGACATTGCCAGCGACTATCTGTGATCTATGCAATTTGCAGACATTAAATCTCTCAAGTTGTAGTGAGCTCAGGGAGTTACCGAGTGGTACTACCAAGTTAATAAATCTGAGACATCTTAACATAGATGATTGTCCAAGACTTGCTAGCATGCCCCCATCAATGAGTATGCTACGACAACTTCAAACTTTGCCGGTATATATCATCGGCCGCAACCCTGAAACTTCTATTGCTCAGCTTGTATCAATGACAAATCTACGAGGGAAGTTAAAACTCAAATGTCTGGAGGAGGCTAAAAGTCCACGTGACGTTGTCATGATAACAGAATGGATGAAAACGAAAGAATTCTATTCATTGGAACTGTTGTGGCAAAATGATGATGAGTGCAAGCTAGATCATAACAGATCTAGGCAAGCTCGCAGGCAAATTGATGATAAAATTGATTTTATTCTGGTAGATTCTTTAACTTTATCTCCCTTTGTAAGGATGTTGTCAATAAATGGTTATTCAGGAACCAAGTTCCCAGATGAGATGAGTTGCTCTCAGAACTTGACCGAGCTAAACATAATAAATTGCAGAAGATGTGAAAGTCTTCCTCCGCTCGGTCAACTCCCTGTCCTCAAGATCCTCAACATCCAAGGAATGCATAATGTAGTCTGCATTGCTATCCCTTATAGATTTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

887

Amino Acids

101.77

Weight (kDa)

8.86

Isoelectric Point (pI)

53.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 96 4.6e-20 Rx N-terminal domain
NB-ARC PF00931 224 - 394 2.3e-37 NB-ARC domain
WHD_DRP PF23559 480 - 553 1.9e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 602 - 873 4.3e-23 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 736 - 874 4.1e-22 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1343
AasI GACNNNNNNGTC 1 cut(s) 221
AatII GACGTC 1 cut(s) 219
Acc16I TGCGCA 1 cut(s) 269
Acc36I ACCTGC 1 cut(s) 1343
AccB7I CCANNNNNTGG 1 cut(s) 1928
AccBSI CCGCTC 1 cut(s) 2578
AccI GTMKAC 2 cut(s) 789, 1236
AciI CCGC 6 cut(s) 23, 143, 448, 1948, 2188, 2576
AclWI GGATC 6 cut(s) 875, 998, 1011, 1471, 1762, 2596
AcoI YGGCCR 1 cut(s) 2185
AcsI RAATTY 6 cut(s) 290, 377, 1365, 1625, 1888, 2321
AcuI CTGAAG 3 cut(s) 744, 1363, 1794
AcvI CACGTG 1 cut(s) 2282
AcyI GRCGYC 2 cut(s) 216, 625
AfaI GTAC 5 cut(s) 394, 613, 930, 971, 2068
AfiI CCNNNNNNNGG 6 cut(s) 746, 1040, 1380, 1928, 2460, 2579
AflIII ACRYGT 2 cut(s) 1318, 1727
AhdI GACNNNNNGTC 1 cut(s) 641
AjnI CCWGG 1 cut(s) 1048
Alw21I GWGCWC 4 cut(s) 347, 984, 1710, 2049
Alw26I GTCTC 6 cut(s) 116, 616, 1201, 1721, 1973, 2083
Alw44I GTGCAC 1 cut(s) 1706
AlwI GGATC 6 cut(s) 875, 998, 1011, 1471, 1762, 2596
AoxI GGCC 2 cut(s) 1532, 2185
ApaLI GTGCAC 1 cut(s) 1706
ApeKI GCWGC 8 cut(s) 25, 28, 31, 34, 166, 903, 1094, 1285
ApoI RAATTY 6 cut(s) 290, 377, 1365, 1625, 1888, 2321
AseI ATTAAT 2 cut(s) 1461, 1647
Asp700I GAANNNNTTC 4 cut(s) 1160, 1845, 2321, 2568
AspLEI GCGC 2 cut(s) 270, 1290
AspS9I GGNCC 2 cut(s) 635, 719
AsuC2I CCSGG 2 cut(s) 860, 1078
AsuHPI GGTGA 8 cut(s) 157, 199, 442, 1208, 1235, 1367, 1812, 1910
AsuNHI GCTAGC 1 cut(s) 2123
AvaII GGWCC 2 cut(s) 635, 719
BaeGI GKGCMC 1 cut(s) 1710
BamHI GGATCC 1 cut(s) 1003
BanII GRGCYC 1 cut(s) 2049
BbrPI CACGTG 1 cut(s) 2282
BbsI GAAGAC 3 cut(s) 699, 1515, 2561
Bbv12I GWGCWC 4 cut(s) 347, 984, 1710, 2049
BbvI GCAGC 8 cut(s) 12, 15, 21, 43, 178, 890, 1106, 1272
BccI CCATC 9 cut(s) 343, 421, 754, 1178, 1382, 1411, 1488, 1601, 2143
BceAI ACGGC 1 cut(s) 282
BcgI CGANNNNNNTGC 2 cut(s) 1195, 1229
BciT130I CCWGG 1 cut(s) 1050
BciVI GTATCC 1 cut(s) 28
BclI TGATCA 1 cut(s) 1639
BcnI CCSGG 2 cut(s) 860, 1078
BcoDI GTCTC 6 cut(s) 116, 616, 1201, 1721, 1973, 2083
BfaI CTAG 9 cut(s) 260, 570, 1104, 1691, 1755, 1976, 2124, 2369, 2385
BfmI CTRYAG 3 cut(s) 29, 1214, 1711
BfuAI ACCTGC 1 cut(s) 1343
BfuI GTATCC 1 cut(s) 28
BglII AGATCT 1 cut(s) 2381
BisI GCNGC 9 cut(s) 26, 29, 32, 35, 167, 904, 1095, 1286, 2188
BlpI GCTNAGC 1 cut(s) 2211
BlsI GCNGC 9 cut(s) 27, 30, 33, 36, 168, 905, 1096, 1287, 2189
Bme1390I CCNGG 3 cut(s) 860, 1050, 1078
Bme18I GGWCC 2 cut(s) 635, 719
BmeRI GACNNNNNGTC 1 cut(s) 641
BmgT120I GGNCC 2 cut(s) 635, 719
BmiI GGNNCC 5 cut(s) 637, 1005, 1045, 1131, 2491
BmrFI CCNGG 3 cut(s) 860, 1050, 1078
BmrI ACTGGG 1 cut(s) 968
BmsI GCATC 6 cut(s) 105, 145, 217, 687, 1339, 1417
BmtI GCTAGC 1 cut(s) 2127
BmuI ACTGGG 1 cut(s) 968
BpiI GAAGAC 3 cut(s) 699, 1515, 2561
BplI GAGNNNNNCTC 2 cut(s) 2503, 2535
BpmI CTGGAG 3 cut(s) 331, 1692, 2282
Bpu10I CCTNAGC 1 cut(s) 2048
Bpu1102I GCTNAGC 1 cut(s) 2211
BpuEI CTTGAG 2 cut(s) 2017, 2582
BpuMI CCSGG 2 cut(s) 860, 1078
BsaAI YACGTR 2 cut(s) 392, 2282
BsaHI GRCGYC 2 cut(s) 216, 625
BsaJI CCNNGG 3 cut(s) 558, 1048, 2614
BsaXI ACNNNNNCTCC 2 cut(s) 2434, 2464
Bsc4I CCNNNNNNNGG 6 cut(s) 746, 1040, 1380, 1928, 2460, 2579
Bse118I RCCGGY 1 cut(s) 2170
Bse1I ACTGG 5 cut(s) 637, 711, 963, 1088, 1549
Bse3DI GCAATG 3 cut(s) 1555, 1982, 2637
BseBI CCWGG 1 cut(s) 1050
BseDI CCNNGG 3 cut(s) 558, 1048, 2614
BseGI GGATG 8 cut(s) 331, 553, 682, 702, 765, 2313, 2469, 2610
BseLI CCNNNNNNNGG 6 cut(s) 746, 1040, 1380, 1928, 2460, 2579
BseMI GCAATG 3 cut(s) 1555, 1982, 2637
BseNI ACTGG 5 cut(s) 637, 711, 963, 1088, 1549
BseRI GAGGAG 2 cut(s) 176, 2279
BseSI GKGCMC 1 cut(s) 1710
BseX3I CGGCCG 1 cut(s) 2185
BseXI GCAGC 8 cut(s) 12, 15, 21, 43, 178, 890, 1106, 1272
Bsh1285I CGRYCG 2 cut(s) 316, 2188
BshFI GGCC 2 cut(s) 1534, 2187
BsiEI CGRYCG 2 cut(s) 316, 2188
BsiHKAI GWGCWC 4 cut(s) 347, 984, 1710, 2049
BsiSI CCGG 3 cut(s) 860, 1078, 2171
BsiWI CGTACG 1 cut(s) 392
BslFI GGGAC 1 cut(s) 1238
BslI CCNNNNNNNGG 6 cut(s) 746, 1040, 1380, 1928, 2460, 2579
BsmAI GTCTC 6 cut(s) 116, 616, 1201, 1721, 1973, 2083
BsmBI CGTCTC 2 cut(s) 116, 616
BsmFI GGGAC 1 cut(s) 1238
BsmI GAATGC 2 cut(s) 1887, 2625
BsnI GGCC 2 cut(s) 1534, 2187
Bsp1286I GDGCHC 4 cut(s) 347, 984, 1710, 2049
Bsp1720I GCTNAGC 1 cut(s) 2211
BspACI CCGC 6 cut(s) 23, 143, 448, 1948, 2188, 2576
BspANI GGCC 2 cut(s) 1534, 2187
BspHI TCATGA 1 cut(s) 2292
BspLI GGNNCC 5 cut(s) 637, 1005, 1045, 1131, 2491
BspMAI CTGCAG 2 cut(s) 33, 1218
BspMI ACCTGC 1 cut(s) 1343
BspOI GCTAGC 1 cut(s) 2127
BspPI GGATC 6 cut(s) 875, 998, 1011, 1471, 1762, 2596
BsrBI CCGCTC 1 cut(s) 2578
BsrDI GCAATG 3 cut(s) 1555, 1982, 2637
BsrFI RCCGGY 1 cut(s) 2170
BsrI ACTGG 5 cut(s) 637, 711, 963, 1088, 1549
BssAI RCCGGY 1 cut(s) 2170
BssECI CCNNGG 3 cut(s) 558, 1048, 2614
BssNI GRCGYC 2 cut(s) 216, 625
BssT1I CCWWGG 2 cut(s) 558, 2614
Bst2UI CCWGG 1 cut(s) 1050
Bst4CI ACNGT 6 cut(s) 61, 357, 506, 606, 1712, 2340
Bst6I CTCTTC 2 cut(s) 666, 1140
BstACI GRCGYC 2 cut(s) 216, 625
BstAPI GCANNNNNTGC 1 cut(s) 227
BstBAI YACGTR 2 cut(s) 392, 2282
BstENI CCTNNNNNAGG 1 cut(s) 2458
BstF5I GGATG 8 cut(s) 331, 553, 682, 702, 765, 2313, 2469, 2610
BstHHI GCGC 2 cut(s) 270, 1290
BstMAI GTCTC 6 cut(s) 116, 616, 1201, 1721, 1973, 2083
BstMCI CGRYCG 2 cut(s) 316, 2188
BstMWI GCNNNNNNNGC 6 cut(s) 31, 34, 205, 227, 1424, 1755
BstNI CCWGG 1 cut(s) 1050
BstNSI RCATGY 3 cut(s) 1322, 1731, 2131
BstSCI CCNGG 3 cut(s) 858, 1048, 1076
BstSFI CTRYAG 3 cut(s) 29, 1214, 1711
BstSLI GKGCMC 1 cut(s) 1710
BstV1I GCAGC 8 cut(s) 12, 15, 21, 43, 178, 890, 1106, 1272
BstV2I GAAGAC 3 cut(s) 699, 1515, 2561
BstX2I RGATCY 3 cut(s) 1003, 2381, 2601
BstXI CCANNNNNNTGG 1 cut(s) 1014
BstYI RGATCY 3 cut(s) 1003, 2381, 2601
BstZI CGGCCG 1 cut(s) 2185
BsuI GTATCC 1 cut(s) 28
BsuRI GGCC 2 cut(s) 1534, 2187
BtgZI GCGATG 1 cut(s) 1389
BtsCI GGATG 8 cut(s) 331, 553, 682, 702, 765, 2313, 2469, 2610
BtsIMutI CAGTG 5 cut(s) 57, 1133, 1542, 1708, 1900
BveI ACCTGC 1 cut(s) 1343
CciI TCATGA 1 cut(s) 2292
CfoI GCGC 2 cut(s) 270, 1290
Cfr10I RCCGGY 1 cut(s) 2170
Cfr13I GGNCC 2 cut(s) 635, 719
CseI GACGC 1 cut(s) 633
Csp6I GTAC 5 cut(s) 393, 612, 929, 970, 2067
CviAII CATG 9 cut(s) 424, 908, 1282, 1319, 1496, 1637, 1728, 2128, 2293
CviQI GTAC 5 cut(s) 393, 612, 929, 970, 2067
DraI TTTAAA 3 cut(s) 1294, 1630, 1870
DrdI GACNNNNNNGTC 1 cut(s) 221
DriI GACNNNNNGTC 1 cut(s) 641
DseDI GACNNNNNNGTC 1 cut(s) 221
EaeI YGGCCR 1 cut(s) 2185
EagI CGGCCG 1 cut(s) 2185
Eam1104I CTCTTC 2 cut(s) 666, 1140
Eam1105I GACNNNNNGTC 1 cut(s) 641
EarI CTCTTC 2 cut(s) 666, 1140
Ecl136II GAGCTC 1 cut(s) 2047
EclXI CGGCCG 1 cut(s) 2185
Eco130I CCWWGG 2 cut(s) 558, 2614
Eco24I GRGCYC 1 cut(s) 2049
Eco32I GATATC 1 cut(s) 320
Eco47I GGWCC 2 cut(s) 635, 719
Eco52I CGGCCG 1 cut(s) 2185
Eco53kI GAGCTC 1 cut(s) 2047
Eco57I CTGAAG 3 cut(s) 744, 1363, 1794
Eco72I CACGTG 1 cut(s) 2282
EcoICRI GAGCTC 1 cut(s) 2047
EcoNI CCTNNNNNAGG 1 cut(s) 2458
EcoRI GAATTC 2 cut(s) 1365, 2321
EcoRII CCWGG 1 cut(s) 1048
EcoRV GATATC 1 cut(s) 320
EcoT14I CCWWGG 2 cut(s) 558, 2614
EcoT22I ATGCAT 2 cut(s) 1638, 2625
EcoT38I GRGCYC 1 cut(s) 2049
ErhI CCWWGG 2 cut(s) 558, 2614
Esp3I CGTCTC 2 cut(s) 116, 616
FaeI CATG 9 cut(s) 427, 911, 1285, 1322, 1499, 1640, 1731, 2131, 2296
FalI AAGNNNNNCTT 2 cut(s) 457, 489
FaqI GGGAC 1 cut(s) 1238
FatI CATG 9 cut(s) 423, 907, 1281, 1318, 1495, 1636, 1727, 2127, 2292
FauI CCCGC 1 cut(s) 30
FbaI TGATCA 1 cut(s) 1639
FblI GTMKAC 2 cut(s) 789, 1236
Fnu4HI GCNGC 9 cut(s) 26, 29, 32, 35, 167, 904, 1095, 1286, 2188
FokI GGATG 8 cut(s) 318, 560, 689, 709, 772, 2320, 2476, 2597
FriOI GRGCYC 1 cut(s) 2049
Fsp4HI GCNGC 9 cut(s) 26, 29, 32, 35, 167, 904, 1095, 1286, 2188
FspBI CTAG 9 cut(s) 260, 570, 1104, 1691, 1755, 1976, 2124, 2369, 2385
FspI TGCGCA 1 cut(s) 269
GlaI GCGC 2 cut(s) 269, 1289
GluI GCNGC 9 cut(s) 26, 29, 32, 35, 167, 904, 1095, 1286, 2188
GsuI CTGGAG 3 cut(s) 331, 1692, 2282
HaeIII GGCC 2 cut(s) 1534, 2187
HapII CCGG 3 cut(s) 860, 1078, 2171
HgaI GACGC 1 cut(s) 633
HhaI GCGC 2 cut(s) 270, 1290
Hin1I GRCGYC 2 cut(s) 216, 625
Hin1II CATG 9 cut(s) 427, 911, 1285, 1322, 1499, 1640, 1731, 2131, 2296
Hin6I GCGC 2 cut(s) 268, 1288
HinP1I GCGC 2 cut(s) 268, 1288
HincII GTYRAC 2 cut(s) 51, 2585
HindII GTYRAC 2 cut(s) 51, 2585
HindIII AAGCTT 1 cut(s) 1416
HpaII CCGG 3 cut(s) 860, 1078, 2171
HphI GGTGA 8 cut(s) 157, 199, 442, 1208, 1235, 1367, 1812, 1910
Hpy166II GTNNAC 7 cut(s) 51, 790, 1237, 1334, 1708, 2279, 2585
Hpy8I GTNNAC 7 cut(s) 51, 790, 1237, 1334, 1708, 2279, 2585
Hpy99I CGWCG 1 cut(s) 221
HpyAV CCTTC 2 cut(s) 39, 184
HpyCH4III ACNGT 6 cut(s) 61, 357, 506, 606, 1712, 2340
HpyCH4IV ACGT 5 cut(s) 125, 216, 391, 2281, 2286
HpyF10VI GCNNNNNNNGC 6 cut(s) 31, 34, 205, 227, 1424, 1755
HpySE526I ACGT 5 cut(s) 125, 216, 391, 2281, 2286
Hsp92I GRCGYC 2 cut(s) 216, 625
Hsp92II CATG 9 cut(s) 427, 911, 1285, 1322, 1499, 1640, 1731, 2131, 2296
HspAI GCGC 2 cut(s) 268, 1288
Ksp22I TGATCA 1 cut(s) 1639
LmnI GCTCC 4 cut(s) 163, 350, 529, 1049
Lsp1109I GCAGC 8 cut(s) 12, 15, 21, 43, 178, 890, 1106, 1272
LweI GCATC 6 cut(s) 105, 145, 217, 687, 1339, 1417
MaeI CTAG 9 cut(s) 260, 570, 1104, 1691, 1755, 1976, 2124, 2369, 2385
MaeII ACGT 5 cut(s) 125, 216, 391, 2281, 2286
MaeIII GTNAC 8 cut(s) 126, 513, 805, 954, 1314, 1388, 2055, 2282
MbiI CCGCTC 1 cut(s) 2578
MfeI CAATTG 1 cut(s) 941
MflI RGATCY 3 cut(s) 1003, 2381, 2601
MhlI GDGCHC 4 cut(s) 347, 984, 1710, 2049
MlyI GAGTC 4 cut(s) 14, 905, 1529, 1681
MmeI TCCRAC 4 cut(s) 336, 727, 874, 1874
Mph1103I ATGCAT 2 cut(s) 1638, 2625
MroXI GAANNNNTTC 4 cut(s) 1160, 1845, 2321, 2568
MslI CAYNNNNRTG 3 cut(s) 340, 428, 1647
MspA1I CMGCKG 2 cut(s) 25, 34
MspI CCGG 3 cut(s) 860, 1078, 2171
MspR9I CCNGG 3 cut(s) 860, 1050, 1078
MunI CAATTG 1 cut(s) 941
Mva1269I GAATGC 2 cut(s) 1887, 2625
MvaI CCWGG 1 cut(s) 1050
MwoI GCNNNNNNNGC 6 cut(s) 31, 34, 205, 227, 1424, 1755
NciI CCSGG 2 cut(s) 860, 1078
NheI GCTAGC 1 cut(s) 2123
NlaIII CATG 9 cut(s) 427, 911, 1285, 1322, 1499, 1640, 1731, 2131, 2296
NlaIV GGNNCC 5 cut(s) 637, 1005, 1045, 1131, 2491
NmuCI GTSAC 2 cut(s) 513, 2282
NsbI TGCGCA 1 cut(s) 269
NsiI ATGCAT 2 cut(s) 1638, 2625
NspI RCATGY 3 cut(s) 1322, 1731, 2131
PaeI GCATGC 1 cut(s) 2131
PagI TCATGA 1 cut(s) 2292
PaqCI CACCTGC 1 cut(s) 1343
PciI ACATGT 2 cut(s) 1318, 1727
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 2 cut(s) 1887, 2625
PdmI GAANNNNTTC 4 cut(s) 1160, 1845, 2321, 2568
PfeI GAWTC 7 cut(s) 397, 650, 950, 1030, 1278, 1679, 2435
Pfl23II CGTACG 1 cut(s) 392
PflFI GACNNNGTC 1 cut(s) 2288
PflMI CCANNNNNTGG 1 cut(s) 1928
PkrI GCNGC 9 cut(s) 27, 30, 33, 36, 168, 905, 1096, 1287, 2189
PleI GAGTC 4 cut(s) 13, 905, 1528, 1681
PmaCI CACGTG 1 cut(s) 2282
PmlI CACGTG 1 cut(s) 2282
PpsI GAGTC 4 cut(s) 13, 905, 1528, 1681
Ppu21I YACGTR 2 cut(s) 392, 2282
PscI ACATGT 2 cut(s) 1318, 1727
PshBI ATTAAT 2 cut(s) 1461, 1647
Psp124BI GAGCTC 1 cut(s) 2049
Psp6I CCWGG 1 cut(s) 1048
PspCI CACGTG 1 cut(s) 2282
PspGI CCWGG 1 cut(s) 1048
PspLI CGTACG 1 cut(s) 392
PspN4I GGNNCC 5 cut(s) 637, 1005, 1045, 1131, 2491
PspPI GGNCC 2 cut(s) 635, 719
PstI CTGCAG 2 cut(s) 33, 1218
PsuI RGATCY 3 cut(s) 1003, 2381, 2601
PsyI GACNNNGTC 1 cut(s) 2288
PvuII CAGCTG 1 cut(s) 34
RsaI GTAC 5 cut(s) 394, 613, 930, 971, 2068
RsaNI GTAC 5 cut(s) 393, 612, 929, 970, 2067
RseI CAYNNNNRTG 3 cut(s) 340, 428, 1647
SacI GAGCTC 1 cut(s) 2049
SatI GCNGC 9 cut(s) 26, 29, 32, 35, 167, 904, 1095, 1286, 2188
Sau96I GGNCC 2 cut(s) 635, 719
SchI GAGTC 4 cut(s) 14, 905, 1529, 1681
ScrFI CCNGG 3 cut(s) 860, 1050, 1078
SduI GDGCHC 4 cut(s) 347, 984, 1710, 2049
SfaNI GCATC 6 cut(s) 105, 145, 217, 687, 1339, 1417
SfcI CTRYAG 3 cut(s) 29, 1214, 1711
SinI GGWCC 2 cut(s) 635, 719
SmiMI CAYNNNNRTG 3 cut(s) 340, 428, 1647
SmlI CTYRAG 2 cut(s) 2032, 2597
SmoI CTYRAG 2 cut(s) 2032, 2597
SphI GCATGC 1 cut(s) 2131
SsiI CCGC 6 cut(s) 23, 143, 448, 1948, 2188, 2576
SspI AATATT 2 cut(s) 1270, 1440
SspMI CTAG 9 cut(s) 260, 570, 1104, 1691, 1755, 1976, 2124, 2369, 2385
SstI GAGCTC 1 cut(s) 2049
StyD4I CCNGG 3 cut(s) 858, 1048, 1076
StyI CCWWGG 2 cut(s) 558, 2614
TaaI ACNGT 6 cut(s) 61, 357, 506, 606, 1712, 2340
TaiI ACGT 5 cut(s) 128, 219, 394, 2284, 2289
TaqI TCGA 4 cut(s) 108, 225, 328, 984
TaqII GACCGA 3 cut(s) 330, 2546, 2570
TatI WGTACW 1 cut(s) 611
TauI GCSGC 1 cut(s) 2190
TfiI GAWTC 7 cut(s) 397, 650, 950, 1030, 1278, 1679, 2435
TscAI CASTG 5 cut(s) 64, 1140, 1549, 1715, 1900
TseFI GTSAC 2 cut(s) 513, 2282
TseI GCWGC 8 cut(s) 25, 28, 31, 34, 166, 903, 1094, 1285
Tsp45I GTSAC 2 cut(s) 513, 2282
TspDTI ATGAA 6 cut(s) 296, 1026, 1043, 1112, 2319, 2324
TspGWI ACGGA 1 cut(s) 116
TspRI CASTG 5 cut(s) 64, 1140, 1549, 1715, 1900
Tth111I GACNNNGTC 1 cut(s) 2288
Van91I CCANNNNNTGG 1 cut(s) 1928
VneI GTGCAC 1 cut(s) 1706
VpaK11BI GGWCC 2 cut(s) 635, 719
VspI ATTAAT 2 cut(s) 1461, 1647
XagI CCTNNNNNAGG 1 cut(s) 2458
XapI RAATTY 6 cut(s) 290, 377, 1365, 1625, 1888, 2321
XbaI TCTAGA 1 cut(s) 1690
XceI RCATGY 3 cut(s) 1322, 1731, 2131
XmiI GTMKAC 2 cut(s) 789, 1236
XmnI GAANNNNTTC 4 cut(s) 1160, 1845, 2321, 2568
XspI CTAG 9 cut(s) 260, 570, 1104, 1691, 1755, 1976, 2124, 2369, 2385
ZraI GACGTC 1 cut(s) 217
Zsp2I ATGCAT 2 cut(s) 1638, 2625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.