Rh6DG159400

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000400
Physical Location & Seq
Forward (+)
23759637 .. 23763468
3832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG159400.1

Sequence Viewer

Length: 2799 bp
ATGGCAGACCTGGTCTTATCCCCAGCTTTGCAAGTGATCTTTGACAGAATAGCATCCCCTGTCTTAGAAAAGATTGCTGACTTGTGGGATCTCAAGGACAACGTCCAGAGGCTAAGAGAGACCTTGATGCTGATTCAACCTACTCTCGAGGATGCTGAAGAGCAACAATTCTCCAACAAAGCTGTCAAAATTTGGCTGTCAAAGCTTGAGAAGGCAGCTTATGATGCTGAGGACGGACTGCAATACATGACTGCTAGAGGTATGAACCGTGACATTTCTGTTCAACTTGATGATCTTATGAATGCTTCCCACATTAAAGAGATGCTTCTGGCATTAGAAACAACTTTGAATGAGGGACTTGTTAAGTTTACTCTTAAAAGGCCTAATATGGTAGATCGTCGATCTTCCATGTGGGAGACTAGCTCTTTTGTCATCAAGTCACAGATATATGGAAGAGATGATGACAAAGATAAGGTCGTGAAACTGTTACTATCTTCTCAAGCCGACCAGGAAGGATATGCATCTTGTATCGCAATAGTTGGTATTGGAGGAATTGGCTTATTATTGGAAGACGCGAGCAAGCGACCTGAAAATGTTGGTGATGAATACTTCACCAATTTGTTGTGGATGTCTTTCTTTCAAGAAGTACATCGATGTGATGGTGGAGGTGTAATTGGGTACAAAATGAATGATGTCATTCATGACCTCGCACAGTATGTTGCAGGGAACACAACCATGATACTTGAAAATGGTCTCCAACCAAAGAATTTAGAAAAACTTCGCCACTCCTCTGTTGTTTATGGATATAGAGCAATTACAATTCCACGAGCTCTGTATGAAGCACAACACCTGCGAAGTCTATTATTTATTGGAGAATCTGGCTTGATAAATTACTTTCTTCTTGATACAATCTCTTCAAGTTTTGTATACTTGCGTATGCTAGACCTGAACAGTTGTGATGTTCATAGATTGCCAGAATCATTGTGCGACTTAATATGTTTGAGATATCTTGACCTGTCTTACACACCTATATTCACGCTTCCTTATGACAGGGCATCGGAACTCGTGTCTCTGCAGACTTTAAACCTACTTGGTTGCCCTAATTTGAGGCGTTTGCCTGACTTGGGAGGCATGACAGGCCTAAGACATCTCGATATAACTGCATGCGCAAGTTTGACTCAGATGCCTAAGGGTATTGGAGAACTACATCAACTTGAGACATTGCCATTGTATGTTATTAGCGACAACAGCGAGGCCCTTGAGCTGGGTGGTTTGAATCTTTATGGCACGTTGAATCTTACACACTTGGAAAATGTAACCAAGGTAGTAGAAGCGAAATCAGCAGGATTGAGGATGAAGGAGAATATCGAGTCATTGGGACTATATTGGAGAGGCGAAGATGTGGATGAATCAATCATGATAAAACTACCTAAAGCCCAACAAGAAGCAGTCAACTCAGGATCTCAGAGAGTGCCACAGAATTCTGAACCCGTGGAAGAAATTCTTGAAGCCTTGAAACCGCACGACAATCTGAAGATGCTAGTTATAAATGGGTATCCAGGAACTAGACTTCCTGATTGGACTCTCCCGAATCTAACTGCAGTTTATTTGAAGGAATGTAGAAATTGTCAGAATCTTCCAGCTCTTGGGAATCTCCTGATGCTTAAAACTCTTTCTTTGCAAGGAATGGATTGTCTGAAACACGTTGGTGCAGATTTCTACGGTGATGATACCAGCATACCATTCTTGTCACTTGAAGAGTTATCGCTCTGTGATTTATCCAACTTGGAAGAGTGGTCGAGTGCAAATAACATGACTTCATTCCCGAGATTGAGGAAGTTAACCGTTAACAGATGTCCCAAGTTGGCACATATACCATTATCTGAATCTCTTCGACATTTGGAGTTGCGGGGTTGTAAACTACGTTTGATGTCCATAGCAAATTTAAGTCTACTTTCTGTGCTCGTCTTAGAAAATATTCCTGACCTATCCTCTCTCCCAGAAGGACTTATTGCATCAGCTCATCTGTCTTGTCTGAAGATTTTGTCATGCCCCAAGCTTCGTTCACTGCCTTTGGAGATGGGAAATTGCCTTACTAGTCTGAAATCATTGACCATTGGTTGGTGTCAAGATCTGTCGTCTCTGCCTGACAGTTTGCAAAACCTCAAAGCCCTGGAATCACTGGAGATTAGTGACTGTCACTACATAATCTCCATGCCGGATGGTGCTATTGGAGGTTTGAGTTCCCTTCGAACTTTGTCCATTGAGAACTGCACTAGTCTGAATTCTTTGTCCTCAAGTTTGGAACATTTGACATTCCTTGAACACTTGACCATTATGTATTGTCCAAGTCTTGGTTCTTTTCCAGAGGGTGTGCAACACCTCTCTGCCCTTCGAAGTTTGACTATCCTGAGCTGTCCTTGGTTTGACTCTCTGCCAAATGGCTTACAAAATGTCAGGACACTACACTGTTTGGAAATTAGTAGCTGCACAAATCTAAGAGCTTTGCCAGAATGGTTTGAGAATCTTAACTCTCTTAGATCCTTGACCATATCTGCTTGTCCTAATTTAGAAGAATTGCCGGCAGGTCTAAAGCATCTCACTAAACTCCAGTACCTCTCTATCCAAGAATGTCCTGAGCTTGAGCAAAGATGCAGACAAGGAAGTGGAGAGGATTGGTTCAAAATAGCTCATGTTCCACATAAATACATTGGTTCTCCTCAGGTCAGTCAATCCAGCGAAGCAAGCACCTCAGGCGGATCGTCTTCTCAAACATCTGCTCACGGCATGAGCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

932

Amino Acids

103.61

Weight (kDa)

5.28

Isoelectric Point (pI)

52.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 6 - 81 4.3e-17 Rx N-terminal domain
WHD_DRP PF23559 186 - 237 6e-10 Disease resistance protein Winged helix domain
LRR_14 PF23598 283 - 614 1.6e-20 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 421 - 563 1.8e-28 R13L1/DRL21 LRRs
LRR_14 PF23598 603 - 730 2.6e-06 Leucine-rich repeat region
LRR_14 PF23598 748 - 877 2.1e-12 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 835 - 878 5.9e-06 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1547
AarI CACCTGC 1 cut(s) 858
Acc16I TGCGCA 1 cut(s) 1168
Acc36I ACCTGC 2 cut(s) 858, 2576
AccB7I CCANNNNNTGG 3 cut(s) 1646, 2121, 2354
AccI GTMKAC 2 cut(s) 927, 1951
AccII CGCG 1 cut(s) 575
AciI CCGC 3 cut(s) 1520, 1909, 2757
AclWI GGATC 4 cut(s) 96, 1468, 2535, 2767
AcsI RAATTY 6 cut(s) 189, 766, 1480, 1500, 1942, 2284
AcuI CTGAAG 3 cut(s) 177, 1553, 2057
AfaI GTAC 3 cut(s) 648, 680, 2615
AfiI CCNNNNNNNGG 5 cut(s) 1124, 1264, 1646, 2121, 2354
AflIII ACRYGT 1 cut(s) 1702
AhlI ACTAGT 2 cut(s) 2096, 2276
AjnI CCWGG 4 cut(s) 9, 507, 1558, 2172
AjuI GAANNNNNNNTTGG 2 cut(s) 2341, 2373
AleI CACNNNNGTG 1 cut(s) 1707
AloI GAACNNNNNNTCC 2 cut(s) 1555, 1587
Alw21I GWGCWC 2 cut(s) 832, 1965
Alw26I GTCTC 6 cut(s) 113, 410, 758, 1074, 1211, 2145
AlwI GGATC 4 cut(s) 96, 1468, 2535, 2767
Ama87I CYCGRG 2 cut(s) 146, 1825
AoxI GGCC 3 cut(s) 380, 1138, 1254
ApeKI GCWGC 2 cut(s) 215, 2487
ApoI RAATTY 6 cut(s) 189, 766, 1480, 1500, 1942, 2284
ArsI GACNNNNNNTTYG 2 cut(s) 459, 491
Asp700I GAANNNNTTC 4 cut(s) 777, 1500, 1890, 1977
AspLEI GCGC 1 cut(s) 1169
AspS9I GGNCC 1 cut(s) 1255
AsuHPI GGTGA 3 cut(s) 604, 611, 1736
AsuII TTCGAA 2 cut(s) 2251, 2395
AvaI CYCGRG 2 cut(s) 146, 1825
AxyI CCTNAGG 3 cut(s) 1188, 2721, 2752
BaeI ACNNNNGTAYC 2 cut(s) 2597, 2630
BanII GRGCYC 1 cut(s) 832
BauI CACGAG 2 cut(s) 825, 1064
BbsI GAAGAC 2 cut(s) 576, 2757
Bbv12I GWGCWC 2 cut(s) 832, 1965
BbvCI CCTCAGC 1 cut(s) 228
BbvI GCAGC 2 cut(s) 227, 2474
BccI CCATC 3 cut(s) 653, 2074, 2216
BcgI CGANNNNNNTGC 2 cut(s) 1142, 1176
BciT130I CCWGG 4 cut(s) 11, 509, 1560, 2174
BciVI GTATCC 1 cut(s) 1566
BcoDI GTCTC 6 cut(s) 113, 410, 758, 1074, 1211, 2145
BcuI ACTAGT 2 cut(s) 2096, 2276
BfaI CTAG 7 cut(s) 255, 420, 941, 1541, 1566, 2097, 2277
BfmI CTRYAG 2 cut(s) 1073, 1599
BfuAI ACCTGC 2 cut(s) 858, 2576
BfuI GTATCC 1 cut(s) 1566
BglII AGATCT 1 cut(s) 2131
BisI GCNGC 2 cut(s) 216, 2488
BlsI GCNGC 2 cut(s) 217, 2489
Bme1390I CCNGG 4 cut(s) 11, 509, 1560, 2174
BmeT110I CYCGRG 2 cut(s) 146, 1825
BmgT120I GGNCC 1 cut(s) 1255
BmrFI CCNGG 4 cut(s) 11, 509, 1560, 2174
BpiI GAAGAC 2 cut(s) 576, 2757
BplI GAGNNNNNCTC 2 cut(s) 407, 439
BpmI CTGGAG 2 cut(s) 2204, 2594
Bpu10I CCTNAGC 3 cut(s) 228, 2411, 2637
Bpu14I TTCGAA 2 cut(s) 2251, 2395
BpuEI CTTGAG 7 cut(s) 77, 227, 483, 1235, 1280, 2281, 2663
Bsa29I ATCGAT 1 cut(s) 652
BsaBI GATNNNNATC 1 cut(s) 520
BsaI GGTCTC 2 cut(s) 113, 758
BsaJI CCNNGG 4 cut(s) 1320, 1491, 2172, 2420
BsaXI ACNNNNNCTCC 2 cut(s) 2195, 2225
Bsc4I CCNNNNNNNGG 5 cut(s) 1124, 1264, 1646, 2121, 2354
Bse118I RCCGGY 1 cut(s) 2581
Bse1I ACTGG 2 cut(s) 2187, 2611
Bse21I CCTNAGG 3 cut(s) 1188, 2721, 2752
Bse3DI GCAATG 1 cut(s) 1220
Bse8I GATNNNNATC 1 cut(s) 520
BseBI CCWGG 4 cut(s) 11, 509, 1560, 2174
BseCI ATCGAT 1 cut(s) 652
BseDI CCNNGG 4 cut(s) 1320, 1491, 2172, 2420
BseGI GGATG 6 cut(s) 53, 157, 633, 1359, 1411, 2227
BseJI GATNNNNATC 1 cut(s) 520
BseLI CCNNNNNNNGG 5 cut(s) 1124, 1264, 1646, 2121, 2354
BseMI GCAATG 1 cut(s) 1220
BseMII CTCAG 8 cut(s) 219, 1193, 1470, 1478, 2402, 2628, 2735, 2766
BseNI ACTGG 2 cut(s) 2187, 2611
BseRI GAGGAG 2 cut(s) 778, 2709
BseXI GCAGC 2 cut(s) 227, 2474
BseYI CCCAGC 2 cut(s) 22, 1264
BsgI GTGCAG 3 cut(s) 1731, 2257, 2473
Bsh1236I CGCG 1 cut(s) 575
BshFI GGCC 3 cut(s) 382, 1140, 1256
BshVI ATCGAT 1 cut(s) 652
BsiHKAI GWGCWC 2 cut(s) 832, 1965
BsiHKCI CYCGRG 2 cut(s) 146, 1825
BsiSI CCGG 2 cut(s) 2219, 2582
BslFI GGGAC 3 cut(s) 369, 1392, 1842
BslI CCNNNNNNNGG 5 cut(s) 1124, 1264, 1646, 2121, 2354
BsmAI GTCTC 6 cut(s) 113, 410, 758, 1074, 1211, 2145
BsmBI CGTCTC 1 cut(s) 2145
BsmFI GGGAC 3 cut(s) 369, 1392, 1842
BsmI GAATGC 1 cut(s) 307
BsnI GGCC 3 cut(s) 382, 1140, 1256
Bso31I GGTCTC 2 cut(s) 113, 758
BsoBI CYCGRG 2 cut(s) 146, 1825
Bsp119I TTCGAA 2 cut(s) 2251, 2395
Bsp1286I GDGCHC 2 cut(s) 832, 1965
Bsp143I GATC 9 cut(s) 36, 88, 292, 394, 401, 1460, 2131, 2540, 2759
BspACI CCGC 3 cut(s) 1520, 1909, 2757
BspANI GGCC 3 cut(s) 382, 1140, 1256
BspCNI CTCAG 8 cut(s) 220, 1192, 1469, 1477, 2403, 2629, 2734, 2765
BspDI ATCGAT 1 cut(s) 652
BspFNI CGCG 1 cut(s) 575
BspHI TCATGA 2 cut(s) 700, 1416
BspMAI CTGCAG 2 cut(s) 1077, 1603
BspMI ACCTGC 2 cut(s) 858, 2576
BspPI GGATC 4 cut(s) 96, 1468, 2535, 2767
BspQI GCTCTTC 1 cut(s) 153
BspT104I TTCGAA 2 cut(s) 2251, 2395
BspTNI GGTCTC 2 cut(s) 113, 758
BsrDI GCAATG 1 cut(s) 1220
BsrFI RCCGGY 1 cut(s) 2581
BsrI ACTGG 2 cut(s) 2187, 2611
BssAI RCCGGY 1 cut(s) 2581
BssECI CCNNGG 4 cut(s) 1320, 1491, 2172, 2420
BssMI GATC 9 cut(s) 36, 88, 292, 394, 401, 1460, 2131, 2540, 2759
BssNAI GTATAC 1 cut(s) 928
BssSI CACGAG 2 cut(s) 825, 1064
BssT1I CCWWGG 2 cut(s) 1320, 2420
Bst1107I GTATAC 1 cut(s) 928
Bst2BI CACGAG 2 cut(s) 825, 1064
Bst2UI CCWGG 4 cut(s) 11, 509, 1560, 2174
Bst4CI ACNGT 9 cut(s) 269, 486, 714, 953, 1724, 1846, 2153, 2198, 2471
Bst6I CTCTTC 6 cut(s) 153, 448, 919, 1752, 1785, 1896
BstBI TTCGAA 2 cut(s) 2251, 2395
BstC8I GCNNGC 5 cut(s) 577, 581, 1165, 2583, 2746
BstDSI CCRYGG 1 cut(s) 1491
BstF5I GGATG 6 cut(s) 53, 157, 633, 1359, 1411, 2227
BstFNI CGCG 1 cut(s) 575
BstHHI GCGC 1 cut(s) 1169
BstKTI GATC 9 cut(s) 39, 91, 295, 397, 404, 1463, 2134, 2543, 2762
BstMAI GTCTC 6 cut(s) 113, 410, 758, 1074, 1211, 2145
BstMBI GATC 9 cut(s) 36, 88, 292, 394, 401, 1460, 2131, 2540, 2759
BstMWI GCNNNNNNNGC 7 cut(s) 202, 224, 1137, 1248, 1340, 2745, 2754
BstNI CCWGG 4 cut(s) 11, 509, 1560, 2174
BstNSI RCATGY 1 cut(s) 1167
BstSCI CCNGG 4 cut(s) 9, 507, 1558, 2172
BstSFI CTRYAG 2 cut(s) 1073, 1599
BstUI CGCG 1 cut(s) 575
BstV1I GCAGC 2 cut(s) 227, 2474
BstV2I GAAGAC 2 cut(s) 576, 2757
BstX2I RGATCY 4 cut(s) 88, 1460, 2131, 2540
BstYI RGATCY 4 cut(s) 88, 1460, 2131, 2540
BstZ17I GTATAC 1 cut(s) 928
Bsu15I ATCGAT 1 cut(s) 652
Bsu36I CCTNAGG 3 cut(s) 1188, 2721, 2752
BsuI GTATCC 1 cut(s) 1566
BsuRI GGCC 3 cut(s) 382, 1140, 1256
BsuTUI ATCGAT 1 cut(s) 652
BtgI CCRYGG 1 cut(s) 1491
BtsCI GGATG 6 cut(s) 53, 157, 633, 1359, 1411, 2227
BtsI GCAGTG 1 cut(s) 2066
BtsIMutI CAGTG 3 cut(s) 2066, 2180, 2467
BveI ACCTGC 2 cut(s) 858, 2576
Cac8I GCNNGC 5 cut(s) 577, 581, 1165, 2583, 2746
CciI TCATGA 2 cut(s) 700, 1416
CfoI GCGC 1 cut(s) 1169
Cfr10I RCCGGY 1 cut(s) 2581
Cfr13I GGNCC 1 cut(s) 1255
ClaI ATCGAT 1 cut(s) 652
CseI GACGC 1 cut(s) 581
CsiI ACCWGGT 1 cut(s) 9
Csp6I GTAC 3 cut(s) 647, 679, 2614
CviQI GTAC 3 cut(s) 647, 679, 2614
DpnI GATC 9 cut(s) 38, 90, 294, 396, 403, 1462, 2133, 2542, 2761
DpnII GATC 9 cut(s) 36, 88, 292, 394, 401, 1460, 2131, 2540, 2759
DraI TTTAAA 1 cut(s) 1083
Eam1104I CTCTTC 6 cut(s) 153, 448, 919, 1752, 1785, 1896
EarI CTCTTC 6 cut(s) 153, 448, 919, 1752, 1785, 1896
EciI GGCGGA 1 cut(s) 2772
Ecl136II GAGCTC 1 cut(s) 830
Eco130I CCWWGG 2 cut(s) 1320, 2420
Eco147I AGGCCT 2 cut(s) 382, 1140
Eco24I GRGCYC 1 cut(s) 832
Eco31I GGTCTC 2 cut(s) 113, 758
Eco32I GATATC 1 cut(s) 1007
Eco53kI GAGCTC 1 cut(s) 830
Eco57I CTGAAG 3 cut(s) 177, 1553, 2057
Eco81I CCTNAGG 3 cut(s) 1188, 2721, 2752
Eco88I CYCGRG 2 cut(s) 146, 1825
EcoICRI GAGCTC 1 cut(s) 830
EcoO109I RGGNCCY 1 cut(s) 1255
EcoRI GAATTC 2 cut(s) 1480, 2284
EcoRII CCWGG 4 cut(s) 9, 507, 1558, 2172
EcoRV GATATC 1 cut(s) 1007
EcoT14I CCWWGG 2 cut(s) 1320, 2420
EcoT22I ATGCAT 1 cut(s) 523
EcoT38I GRGCYC 1 cut(s) 832
ErhI CCWWGG 2 cut(s) 1320, 2420
Esp3I CGTCTC 1 cut(s) 2145
FalI AAGNNNNNCTT 4 cut(s) 309, 341, 1488, 1520
FaqI GGGAC 3 cut(s) 369, 1392, 1842
FauI CCCGC 1 cut(s) 1902
FblI GTMKAC 2 cut(s) 927, 1951
Fnu4HI GCNGC 2 cut(s) 216, 2488
FokI GGATG 6 cut(s) 40, 164, 640, 1366, 1418, 2234
FriOI GRGCYC 1 cut(s) 832
Fsp4HI GCNGC 2 cut(s) 216, 2488
FspBI CTAG 7 cut(s) 255, 420, 941, 1541, 1566, 2097, 2277
FspI TGCGCA 1 cut(s) 1168
GlaI GCGC 1 cut(s) 1168
GluI GCNGC 2 cut(s) 216, 2488
GsaI CCCAGC 2 cut(s) 26, 1268
GsuI CTGGAG 2 cut(s) 2204, 2594
HaeIII GGCC 3 cut(s) 382, 1140, 1256
HapII CCGG 2 cut(s) 2219, 2582
HgaI GACGC 1 cut(s) 581
HhaI GCGC 1 cut(s) 1169
Hin6I GCGC 1 cut(s) 1167
HinP1I GCGC 1 cut(s) 1167
HincII GTYRAC 3 cut(s) 1453, 1842, 1849
HindII GTYRAC 3 cut(s) 1453, 1842, 1849
HindIII AAGCTT 2 cut(s) 203, 2057
HpaI GTTAAC 2 cut(s) 1842, 1849
HpaII CCGG 2 cut(s) 2219, 2582
HphI GGTGA 3 cut(s) 604, 611, 1736
Hpy166II GTNNAC 8 cut(s) 369, 928, 1453, 1842, 1849, 1919, 1952, 2066
Hpy8I GTNNAC 8 cut(s) 369, 928, 1453, 1842, 1849, 1919, 1952, 2066
Hpy99I CGWCG 1 cut(s) 402
HpyAV CCTTC 7 cut(s) 205, 506, 1351, 1606, 1997, 2258, 2402
HpyCH4III ACNGT 9 cut(s) 269, 486, 714, 953, 1724, 1846, 2153, 2198, 2471
HpyCH4IV ACGT 4 cut(s) 102, 1289, 1704, 1924
HpyF10VI GCNNNNNNNGC 7 cut(s) 202, 224, 1137, 1248, 1340, 2745, 2754
HpySE526I ACGT 4 cut(s) 102, 1289, 1704, 1924
HspAI GCGC 1 cut(s) 1167
KroI GCCGGC 1 cut(s) 2581
KroNI GCCGGC 1 cut(s) 2583
KspAI GTTAAC 2 cut(s) 1842, 1849
Kzo9I GATC 9 cut(s) 36, 88, 292, 394, 401, 1460, 2131, 2540, 2759
LguI GCTCTTC 1 cut(s) 153
Lsp1109I GCAGC 2 cut(s) 227, 2474
MabI ACCWGGT 1 cut(s) 9
MaeI CTAG 7 cut(s) 255, 420, 941, 1541, 1566, 2097, 2277
MaeII ACGT 4 cut(s) 102, 1289, 1704, 1924
MaeIII GTNAC 7 cut(s) 269, 438, 486, 1315, 1749, 2192, 2198
MalI GATC 9 cut(s) 38, 90, 294, 396, 403, 1462, 2133, 2542, 2761
MboI GATC 9 cut(s) 36, 88, 292, 394, 401, 1460, 2131, 2540, 2759
MflI RGATCY 4 cut(s) 88, 1460, 2131, 2540
MhlI GDGCHC 2 cut(s) 832, 1965
MlyI GAGTC 4 cut(s) 1171, 1379, 1576, 2423
MmeI TCCRAC 3 cut(s) 198, 781, 1806
Mph1103I ATGCAT 1 cut(s) 523
MroNI GCCGGC 1 cut(s) 2581
MroXI GAANNNNTTC 4 cut(s) 777, 1500, 1890, 1977
MslI CAYNNNNRTG 3 cut(s) 654, 734, 1707
MspI CCGG 2 cut(s) 2219, 2582
MspR9I CCNGG 4 cut(s) 11, 509, 1560, 2174
Mva1269I GAATGC 1 cut(s) 307
MvaI CCWGG 4 cut(s) 11, 509, 1560, 2174
MvnI CGCG 1 cut(s) 575
MwoI GCNNNNNNNGC 7 cut(s) 202, 224, 1137, 1248, 1340, 2745, 2754
NaeI GCCGGC 1 cut(s) 2583
NdeII GATC 9 cut(s) 36, 88, 292, 394, 401, 1460, 2131, 2540, 2759
NgoMIV GCCGGC 1 cut(s) 2581
NmuCI GTSAC 5 cut(s) 269, 438, 1749, 2192, 2198
NsbI TGCGCA 1 cut(s) 1168
NsiI ATGCAT 1 cut(s) 523
NspI RCATGY 1 cut(s) 1167
NspV TTCGAA 2 cut(s) 2251, 2395
OliI CACNNNNGTG 1 cut(s) 1707
PaeI GCATGC 1 cut(s) 1167
PaeR7I CTCGAG 1 cut(s) 146
PagI TCATGA 2 cut(s) 700, 1416
PaqCI CACCTGC 1 cut(s) 858
PceI AGGCCT 2 cut(s) 382, 1140
PciSI GCTCTTC 1 cut(s) 153
PctI GAATGC 1 cut(s) 307
PdiI GCCGGC 1 cut(s) 2583
PdmI GAANNNNTTC 4 cut(s) 777, 1500, 1890, 1977
PflFI GACNNNGTC 2 cut(s) 11, 101
PflMI CCANNNNNTGG 3 cut(s) 1646, 2121, 2354
PfoI TCCNGGA 1 cut(s) 1558
PkrI GCNGC 2 cut(s) 217, 2489
PleI GAGTC 4 cut(s) 1171, 1378, 1576, 2423
PpsI GAGTC 4 cut(s) 1171, 1378, 1576, 2423
PsiI TTATAA 1 cut(s) 1547
Psp124BI GAGCTC 1 cut(s) 832
Psp6I CCWGG 4 cut(s) 9, 507, 1558, 2172
PspFI CCCAGC 2 cut(s) 22, 1264
PspGI CCWGG 4 cut(s) 9, 507, 1558, 2172
PspPI GGNCC 1 cut(s) 1255
PstI CTGCAG 2 cut(s) 1077, 1603
PsuI RGATCY 4 cut(s) 88, 1460, 2131, 2540
PsyI GACNNNGTC 2 cut(s) 11, 101
RsaI GTAC 3 cut(s) 648, 680, 2615
RsaNI GTAC 3 cut(s) 647, 679, 2614
RseI CAYNNNNRTG 3 cut(s) 654, 734, 1707
SacI GAGCTC 1 cut(s) 832
SapI GCTCTTC 1 cut(s) 153
SatI GCNGC 2 cut(s) 216, 2488
Sau3AI GATC 9 cut(s) 36, 88, 292, 394, 401, 1460, 2131, 2540, 2759
Sau96I GGNCC 1 cut(s) 1255
SchI GAGTC 4 cut(s) 1171, 1379, 1576, 2423
ScrFI CCNGG 4 cut(s) 11, 509, 1560, 2174
SduI GDGCHC 2 cut(s) 832, 1965
SexAI ACCWGGT 1 cut(s) 9
SfcI CTRYAG 2 cut(s) 1073, 1599
Sfr274I CTCGAG 1 cut(s) 146
SfuI TTCGAA 2 cut(s) 2251, 2395
SlaI CTCGAG 1 cut(s) 146
SmiMI CAYNNNNRTG 3 cut(s) 654, 734, 1707
SmlI CTYRAG 8 cut(s) 92, 146, 206, 498, 1214, 1259, 2296, 2642
SmoI CTYRAG 8 cut(s) 92, 146, 206, 498, 1214, 1259, 2296, 2642
SpeI ACTAGT 2 cut(s) 2096, 2276
SphI GCATGC 1 cut(s) 1167
SseBI AGGCCT 2 cut(s) 382, 1140
SsiI CCGC 3 cut(s) 1520, 1909, 2757
SspI AATATT 1 cut(s) 1978
SspMI CTAG 7 cut(s) 255, 420, 941, 1541, 1566, 2097, 2277
SstI GAGCTC 1 cut(s) 832
StuI AGGCCT 2 cut(s) 382, 1140
StyD4I CCNGG 4 cut(s) 9, 507, 1558, 2172
StyI CCWWGG 2 cut(s) 1320, 2420
TaaI ACNGT 9 cut(s) 269, 486, 714, 953, 1724, 1846, 2153, 2198, 2471
TaiI ACGT 4 cut(s) 105, 1292, 1707, 1927
TaqI TCGA 9 cut(s) 147, 400, 652, 1152, 1368, 1799, 1894, 2251, 2395
TatI WGTACW 1 cut(s) 646
TscAI CASTG 3 cut(s) 2073, 2187, 2474
TseFI GTSAC 5 cut(s) 269, 438, 1749, 2192, 2198
TseI GCWGC 2 cut(s) 215, 2487
Tsp45I GTSAC 5 cut(s) 269, 438, 1749, 2192, 2198
TspGWI ACGGA 1 cut(s) 249
TspRI CASTG 3 cut(s) 2073, 2187, 2474
Tth111I GACNNNGTC 2 cut(s) 11, 101
Van91I CCANNNNNTGG 3 cut(s) 1646, 2121, 2354
XapI RAATTY 6 cut(s) 189, 766, 1480, 1500, 1942, 2284
XceI RCATGY 1 cut(s) 1167
XhoI CTCGAG 1 cut(s) 146
XmiI GTMKAC 2 cut(s) 927, 1951
XmnI GAANNNNTTC 4 cut(s) 777, 1500, 1890, 1977
XspI CTAG 7 cut(s) 255, 420, 941, 1541, 1566, 2097, 2277
Zsp2I ATGCAT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.