Rroxscaffold_7G00198730

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000400
Physical Location & Seq
Reverse (-)
44401857 .. 44407641
5785 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00198730.1

Sequence Viewer

Length: 3537 bp
ATGGCAGACCTGGTCTTATGCCCAGCTCTTCAAGTGATCTTTGACAGAATAGCATCCCCTGTCTTAGAAAAGTTTGCTGACTTGTGGGGTCTCAAGGACAACCTCCAGAGCCTAAGAGAGACCTTGATGCTGATTCAACCTACTCTCGAGGATGCGGAAGAGCAACAATTCTCCAACAAAGCTGTCAAAATTTGGTTGTCAAAGCTCGAGAAGGCAGCTTATGATGCTGAGGACGGACTGCACTACTTGACAGCTAAAGGTATGATCCCTAGCAGTTGTTTTCAACCTCTGGATTTTGATCTTATGACTGGTTCCCACATTAAAGAGATGCTTCTGGCATTAGAAACAACTGTAAATGAGGGACTTGTTAAGTTTAATTTTACAAGGCTTAATATGGTAGATCGTCGTTCTTCCCTGTGGGAGACTAGCTCTTTTGTCATCAAGTCACAGATATATGGAAGAGATGATGACAAAGATAAGGTCGTGAAACTGTTGCTGTCTTCTCAAGCTTACCAGGAAGGATATGCATCTTGTATCGCAATAGTTGGTATTGGAGGAATTGGTAAGACCACTCTTGCTCAATTAGCATATAATGACGAGAGAGTAATCCAAAACTTCGATGTTAGGGTGTGGATTTATGTCTCTGATGATTTCAATGTCAAGAAGATCATGAAGACAATTATCGAGTCAGTAACGAACGATGAGTGCAGGTTCTCAGACATTGAACTTCTTCAGTCTCAGCTTTGGCGTTTGCTGCACAATAAAAGATACCTCCTCGTGTTGGATGATGTTTGGACTGCGCATCACAATGACTGGGACAAACTAAAATCTTTGTTTAGAGGGGGTGTTGATGGATGCAAAGTCATTGTTACCACCCGCAATACAAAAACCGCACTCATGACAGACTCTCCAAATTCCCCTTTTTATTTGAAGGGCTTAGAGGAGGAGGATTGCTGGGCTTTGTTCAAGCAACGGGCTTTTGGGCGAGGAGAAGAAGAGAAATATCCACACCTTTTGCCAATTGGGAAGCAGATTGTCAAAAAATGTGGGGGTGTGCCATTAGCAGCAAGAAGTTTGGGAAGTGTAATGCGCTTCAAAAGAGATCAACAACAGTGGCTGTTTATGCAAAATACTGAACTCTGGAAGTTGGATGTGGGCGAACTAGACATTCTTCCTGCCCTGATGTTGAGCTACTTTTACCTACCATTTCATCTTAGAAGATGCTTTGCCTTCTGCTCAATATTTCCAAAAGGTTATGAATTCAACAAGCACAAGTTAATTCATCTGTGGATGGCAGAAGGCTTATTATTGGAAGACGGTAGCAAGAGACCTGAAAATGTTGGTGATGAATACTTCACCCATTTGTTGTGGATGTCTTTCTTTCAAGATGTAGATCGATGTGATGGTGGAGGTGTAATTGGGTACAAAATGAATGATGTCATTCATGGCCTCTCACAGTATGTTTCAGGGAACACAACCATGATACTTGAAAATGGTCTCCAACCAAAGAGTTTAGAAAAAATTCGCCACTCCTCTGTTGTTTATAGATATAGAGCAATCACAATTCCACGGGCTCTGTATGAAGCACAACACCTACGAAGTCTGTTATTTATTGGAGAATCTGGCTTGATAAAGAACATTCATAAAATCTGTTCAAGTTTTGTATACTTGCGTATGCTAGATCTGCACAGTTGTGATGTTCATATATTGCCAAAATCATTGTGCGACTTAATATGTTTGAGATATCTTGACCTGTCTTACACACCTATATTCACGCTTCCTGATTACAATTCATGTAACCTCGTGTCTCTGCAGACTTTAAACCTACTTGGTTGCCCTAATCTGAAGCGTTTGCCTTACTTGGGAGGCATGACAGGCCTAAGACATCTTGATATAACTGCATGCAGAAGTTTGACTCGGATGCCTAAGGGTTTTGGAGAACTACATCAACTTGAGACATTGCCATTGTATGTTATTAGCTACGACGGCGAGGCCTTTGAGCTGGGTGGTTTGAATCTTTATGGCACGTTGAATCTCACACACTTGGAAAATGTAACCAAGGCAGTAGAAGCGAAATCAGCACGATTGAGGATGAAGGAGAATATCGAGTCATTGGGACTATATTGGAAAGGCGAAGATGTGGATGAATCAATCATGATAAAAGTACCTAAAGCCCAACAAGAAGCAGTCATCTCAGGATCACAGAGACTGCCACAGAATTCTGACCCTGTGGAAGAAATTCTTGAAGCCTTGAAACCGCACGACAATTTGAAGATGCTAGTTATAAATGGGTATCCAGGATCTAGACTTCCTGATTGGACTCTCCCGAATCTAACTGCAGTTTATTTGAAGGAATGTAGAAATTGTCAGAATCTTCCAGCTCTTGGGAATCTCCTGATGCTTAAAACTCTTTCTTTGCAAGGAATGGATAGTCTGAAACACGTTGGTGCAGATTTCTATGGTGATGGTACCAGCATACCATTCTTGTCACTTGAAGAGTTATCGCTCTGTGATTTATCCAACTTGGAAGAGTGGTCGAGTGCAAATAACATGACTTCATTCCCAAGATTGAGGAAGTTAACCGTTAACAGATGTCCCAAGTTAGCACATATACCATTATCTGAATCTCTTCGACATTTGGAGTTGCGGGGTTGTAAACTACGTTTGATGTCCATAGAAAATTTAAGTTTACTTTCTGTGCTCGTCTTAGAAAATATTCCAGACCTATCCTCTCTCCCAGAAGGACTTATTGCATCAGCTCATCTGTCTTGTCTGAAGATTTTGTCATGCCCCAAGCTTCGTTCGCTGCCTTTGGAGATGGGAAATTGCCTTACTAGTCTGAAATCATTGACCATTGGTTGGTGTCAAGATCTTTTGTCTCTGCCTGAGAGTTTGCAAAACCTCAAAACCCTGGAATCACTGGAGATTAGTGACTGTCACTACATAATCTCCATGCCGGATGGTGCTATTGGAGGTTTGAGTTCCCTTCGAACTTTGTCCATTGAGAACTGCACTAGTCTGACTTCCTTGTCCTCGAGTTTGGAACATTTGACATTCCTTGAACACTTGACCATTATGTATTGTCCAAGTCTTGATTCTTTTCCAGAGGGTGTGCATCACCTCTCTGCCCTTCGAAGTTTGACTATCCTGAGCTGTCCTTGGTTTGACTCTCTGCCAAATGGCTTACAAAATGTCAGGACACTGCACTGTTTGGAAATTAGTAGCTGCACAAATCTAAGAGCTTTGCCAGAATGGTTTGAGAATCTTGACTCTCTTAGATCCTTGGCCATATCTGCTTGTCCTAATTTAGAAGAATTGCCGGCAGGTCTAAAGCATCTCACTAAACTCCAGTACCTCTCTATCCAAGAATGTCCTGAGCTTGAGCAAAGATGCAGGCAAGGAAGTGGAGAGGATTGGTTCAAAATAGCTCATGTTCCACATAAATACATTGGTTCTCCTCAGGTCAGTCAATCCGGCGAAGCAAGCACCTCAGGCGGATCGTCTTCTCAAACATCTGCTCACGGCATGAGCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1178

Amino Acids

132.67

Weight (kDa)

6.01

Isoelectric Point (pI)

53.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 80 5.3e-17 Rx N-terminal domain
NB-ARC PF00931 155 - 328 2.7e-39 NB-ARC domain
AAA_22 PF13401 175 - 284 2.9e-06 AAA domain
WHD_DRP PF23559 414 - 484 4.8e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 531 - 837 5.3e-21 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 667 - 809 1.6e-28 R13L1/DRL21 LRRs
LRR_14 PF23598 994 - 1123 4.2e-12 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 1061 - 1124 7.1e-07 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2285
AasI GACNNNNNNGTC 1 cut(s) 3028
Acc16I TGCGCA 1 cut(s) 801
Acc36I ACCTGC 2 cut(s) 699, 3314
Acc65I GGTACC 1 cut(s) 2468
AccB1I GGYRCC 1 cut(s) 2468
AccB7I CCANNNNNTGG 2 cut(s) 2384, 2859
AccI GTMKAC 1 cut(s) 1665
AciI CCGC 6 cut(s) 155, 877, 891, 2258, 2647, 3495
AclWI GGATC 5 cut(s) 259, 2206, 2308, 3273, 3505
AcoI YGGCCR 1 cut(s) 3285
AcsI RAATTY 7 cut(s) 189, 913, 1259, 1521, 2218, 2238, 2680
AcuI CTGAAG 3 cut(s) 716, 1865, 2795
AfaI GTAC 4 cut(s) 1424, 2166, 2470, 3353
AfiI CCNNNNNNNGG 4 cut(s) 781, 1862, 2384, 2859
AflIII ACRYGT 1 cut(s) 2440
AhlI ACTAGT 2 cut(s) 2834, 3014
AjnI CCWGG 4 cut(s) 9, 513, 2296, 2910
AjuI GAANNNNNNNTTGG 2 cut(s) 3079, 3111
AleI CACNNNNGTG 2 cut(s) 1692, 2445
Alw21I GWGCWC 1 cut(s) 2703
AlwI GGATC 5 cut(s) 259, 2206, 2308, 3273, 3505
AlwNI CAGNNNCTG 2 cut(s) 1117, 2209
Ama87I CYCGRG 3 cut(s) 146, 206, 3034
AoxI GGCC 4 cut(s) 1447, 1876, 1992, 3285
ApeKI GCWGC 5 cut(s) 215, 754, 1064, 2806, 3225
ApoI RAATTY 7 cut(s) 189, 913, 1259, 1521, 2218, 2238, 2680
ArsI GACNNNNNNTTYG 2 cut(s) 465, 497
Asp700I GAANNNNTTC 5 cut(s) 729, 1521, 2238, 2628, 2715
Asp718I GGTACC 1 cut(s) 2468
AspLEI GCGC 2 cut(s) 802, 1092
AsuHPI GGTGA 4 cut(s) 1348, 1355, 2474, 3110
AsuII TTCGAA 2 cut(s) 2989, 3133
AvaI CYCGRG 3 cut(s) 146, 206, 3034
AxyI CCTNAGG 3 cut(s) 1926, 3459, 3490
BaeI ACNNNNGTAYC 2 cut(s) 3335, 3368
BalI TGGCCA 1 cut(s) 3287
BanI GGYRCC 1 cut(s) 2468
BanII GRGCYC 1 cut(s) 1576
BauI CACGAG 2 cut(s) 776, 1802
BbsI GAAGAC 4 cut(s) 492, 680, 1320, 3495
Bbv12I GWGCWC 1 cut(s) 2703
BbvCI CCTCAGC 1 cut(s) 228
BbvI GCAGC 5 cut(s) 227, 741, 1076, 2793, 3212
BccI CCATC 6 cut(s) 845, 1285, 1397, 2459, 2812, 2954
BceAI ACGGC 1 cut(s) 2002
BciT130I CCWGG 4 cut(s) 11, 515, 2298, 2912
BciVI GTATCC 1 cut(s) 2304
BcuI ACTAGT 2 cut(s) 2834, 3014
BfaI CTAG 8 cut(s) 270, 426, 1163, 1679, 2279, 2304, 2835, 3015
BfmI CTRYAG 2 cut(s) 1811, 2337
BfuAI ACCTGC 2 cut(s) 699, 3314
BfuI GTATCC 1 cut(s) 2304
BglII AGATCT 2 cut(s) 1681, 2869
BisI GCNGC 5 cut(s) 216, 755, 1065, 2807, 3226
BlsI GCNGC 5 cut(s) 217, 756, 1066, 2808, 3227
Bme1390I CCNGG 4 cut(s) 11, 515, 2298, 2912
BmeT110I CYCGRG 3 cut(s) 146, 206, 3034
BmiI GGNNCC 2 cut(s) 313, 2470
BmrFI CCNGG 4 cut(s) 11, 515, 2298, 2912
BmrI ACTGGG 1 cut(s) 823
BmuI ACTGGG 1 cut(s) 823
BpiI GAAGAC 4 cut(s) 492, 680, 1320, 3495
BplI GAGNNNNNCTC 2 cut(s) 413, 445
BpmI CTGGAG 3 cut(s) 89, 2942, 3332
Bpu10I CCTNAGC 3 cut(s) 228, 3149, 3375
Bpu14I TTCGAA 2 cut(s) 2989, 3133
BpuEI CTTGAG 4 cut(s) 77, 489, 1973, 3401
Bsa29I ATCGAT 1 cut(s) 1396
BsaBI GATNNNNATC 3 cut(s) 297, 526, 1392
BsaI GGTCTC 4 cut(s) 95, 113, 1321, 1502
BsaJI CCNNGG 5 cut(s) 1568, 2058, 2910, 3158, 3282
BsaXI ACNNNNNCTCC 4 cut(s) 892, 922, 2933, 2963
Bsc4I CCNNNNNNNGG 4 cut(s) 781, 1862, 2384, 2859
Bse118I RCCGGY 1 cut(s) 3319
Bse1I ACTGG 4 cut(s) 313, 818, 2925, 3349
Bse21I CCTNAGG 3 cut(s) 1926, 3459, 3490
Bse3DI GCAATG 1 cut(s) 1958
Bse8I GATNNNNATC 3 cut(s) 297, 526, 1392
BseBI CCWGG 4 cut(s) 11, 515, 2298, 2912
BseCI ATCGAT 1 cut(s) 1396
BseDI CCNNGG 5 cut(s) 1568, 2058, 2910, 3158, 3282
BseJI GATNNNNATC 3 cut(s) 297, 526, 1392
BseLI CCNNNNNNNGG 4 cut(s) 781, 1862, 2384, 2859
BseMI GCAATG 1 cut(s) 1958
BseMII CTCAG 9 cut(s) 219, 729, 752, 2208, 2877, 3140, 3366, 3473, 3504
BseNI ACTGG 4 cut(s) 313, 818, 2925, 3349
BseRI GAGGAG 6 cut(s) 764, 956, 959, 1002, 1522, 3447
BseXI GCAGC 5 cut(s) 227, 741, 1076, 2793, 3212
BseYI CCCAGC 3 cut(s) 22, 954, 2002
BsgI GTGCAG 8 cut(s) 224, 727, 740, 1670, 2469, 2995, 3188, 3211
BshFI GGCC 4 cut(s) 1449, 1878, 1994, 3287
BshNI GGYRCC 1 cut(s) 2468
BshVI ATCGAT 1 cut(s) 1396
BsiHKAI GWGCWC 1 cut(s) 2703
BsiHKCI CYCGRG 3 cut(s) 146, 206, 3034
BsiSI CCGG 3 cut(s) 2957, 3320, 3474
BslFI GGGAC 4 cut(s) 375, 830, 2130, 2580
BslI CCNNNNNNNGG 4 cut(s) 781, 1862, 2384, 2859
BsmFI GGGAC 4 cut(s) 375, 830, 2130, 2580
BsnI GGCC 4 cut(s) 1449, 1878, 1994, 3287
Bso31I GGTCTC 4 cut(s) 95, 113, 1321, 1502
BsoBI CYCGRG 3 cut(s) 146, 206, 3034
Bsp119I TTCGAA 2 cut(s) 2989, 3133
Bsp1286I GDGCHC 2 cut(s) 1576, 2703
BspACI CCGC 6 cut(s) 155, 877, 891, 2258, 2647, 3495
BspANI GGCC 4 cut(s) 1449, 1878, 1994, 3287
BspCNI CTCAG 9 cut(s) 220, 728, 751, 2207, 2878, 3141, 3367, 3472, 3503
BspDI ATCGAT 1 cut(s) 1396
BspHI TCATGA 3 cut(s) 669, 897, 2154
BspLI GGNNCC 2 cut(s) 313, 2470
BspMAI CTGCAG 2 cut(s) 1815, 2341
BspMI ACCTGC 2 cut(s) 699, 3314
BspPI GGATC 5 cut(s) 259, 2206, 2308, 3273, 3505
BspQI GCTCTTC 2 cut(s) 33, 153
BspT104I TTCGAA 2 cut(s) 2989, 3133
BspT107I GGYRCC 1 cut(s) 2468
BspTNI GGTCTC 4 cut(s) 95, 113, 1321, 1502
BsrDI GCAATG 1 cut(s) 1958
BsrFI RCCGGY 1 cut(s) 3319
BsrI ACTGG 4 cut(s) 313, 818, 2925, 3349
BssAI RCCGGY 1 cut(s) 3319
BssECI CCNNGG 5 cut(s) 1568, 2058, 2910, 3158, 3282
BssNAI GTATAC 1 cut(s) 1666
BssSI CACGAG 2 cut(s) 776, 1802
BssT1I CCWWGG 3 cut(s) 2058, 3158, 3282
Bst1107I GTATAC 1 cut(s) 1666
Bst2BI CACGAG 2 cut(s) 776, 1802
Bst2UI CCWGG 4 cut(s) 11, 515, 2298, 2912
Bst4CI ACNGT 9 cut(s) 352, 492, 1113, 1319, 1458, 1691, 2584, 2936, 3209
Bst6I CTCTTC 7 cut(s) 33, 153, 454, 990, 2490, 2523, 2634
BstBI TTCGAA 2 cut(s) 2989, 3133
BstC8I GCNNGC 4 cut(s) 1903, 3321, 3395, 3484
BstDSI CCRYGG 1 cut(s) 1568
BstHHI GCGC 2 cut(s) 802, 1092
BstNI CCWGG 4 cut(s) 11, 515, 2298, 2912
BstNSI RCATGY 1 cut(s) 1905
BstSCI CCNGG 4 cut(s) 9, 513, 2296, 2910
BstSFI CTRYAG 2 cut(s) 1811, 2337
BstV1I GCAGC 5 cut(s) 227, 741, 1076, 2793, 3212
BstV2I GAAGAC 4 cut(s) 492, 680, 1320, 3495
BstX2I RGATCY 4 cut(s) 1681, 2300, 2869, 3278
BstYI RGATCY 4 cut(s) 1681, 2300, 2869, 3278
BstZ17I GTATAC 1 cut(s) 1666
Bsu15I ATCGAT 1 cut(s) 1396
Bsu36I CCTNAGG 3 cut(s) 1926, 3459, 3490
BsuI GTATCC 1 cut(s) 2304
BsuRI GGCC 4 cut(s) 1449, 1878, 1994, 3287
BsuTUI ATCGAT 1 cut(s) 1396
BtgI CCRYGG 1 cut(s) 1568
BtsI GCAGTG 1 cut(s) 3200
BtsIMutI CAGTG 4 cut(s) 1118, 2918, 3200, 3205
BveI ACCTGC 2 cut(s) 699, 3314
Cac8I GCNNGC 4 cut(s) 1903, 3321, 3395, 3484
CaiI CAGNNNCTG 2 cut(s) 1117, 2209
CciI TCATGA 3 cut(s) 669, 897, 2154
CfoI GCGC 2 cut(s) 802, 1092
Cfr10I RCCGGY 1 cut(s) 3319
ClaI ATCGAT 1 cut(s) 1396
CsiI ACCWGGT 1 cut(s) 9
Csp6I GTAC 4 cut(s) 1423, 2165, 2469, 3352
CspCI CAANNNNNGTGG 6 cut(s) 996, 1027, 1031, 1062, 1094, 1129
CviQI GTAC 4 cut(s) 1423, 2165, 2469, 3352
DraI TTTAAA 1 cut(s) 1821
DrdI GACNNNNNNGTC 1 cut(s) 3028
DseDI GACNNNNNNGTC 1 cut(s) 3028
EaeI YGGCCR 1 cut(s) 3285
Eam1104I CTCTTC 7 cut(s) 33, 153, 454, 990, 2490, 2523, 2634
EarI CTCTTC 7 cut(s) 33, 153, 454, 990, 2490, 2523, 2634
EciI GGCGGA 1 cut(s) 3510
Eco130I CCWWGG 3 cut(s) 2058, 3158, 3282
Eco147I AGGCCT 2 cut(s) 1878, 1994
Eco24I GRGCYC 1 cut(s) 1576
Eco31I GGTCTC 4 cut(s) 95, 113, 1321, 1502
Eco32I GATATC 1 cut(s) 1745
Eco57I CTGAAG 3 cut(s) 716, 1865, 2795
Eco81I CCTNAGG 3 cut(s) 1926, 3459, 3490
Eco88I CYCGRG 3 cut(s) 146, 206, 3034
EcoRI GAATTC 2 cut(s) 1259, 2218
EcoRII CCWGG 4 cut(s) 9, 513, 2296, 2910
EcoRV GATATC 1 cut(s) 1745
EcoT14I CCWWGG 3 cut(s) 2058, 3158, 3282
EcoT22I ATGCAT 1 cut(s) 529
EcoT38I GRGCYC 1 cut(s) 1576
ErhI CCWWGG 3 cut(s) 2058, 3158, 3282
FalI AAGNNNNNCTT 4 cut(s) 315, 347, 2226, 2258
FaqI GGGAC 4 cut(s) 375, 830, 2130, 2580
FauI CCCGC 2 cut(s) 884, 2640
FblI GTMKAC 1 cut(s) 1665
Fnu4HI GCNGC 5 cut(s) 216, 755, 1065, 2807, 3226
FriOI GRGCYC 1 cut(s) 1576
Fsp4HI GCNGC 5 cut(s) 216, 755, 1065, 2807, 3226
FspBI CTAG 8 cut(s) 270, 426, 1163, 1679, 2279, 2304, 2835, 3015
FspI TGCGCA 1 cut(s) 801
GlaI GCGC 2 cut(s) 801, 1091
GluI GCNGC 5 cut(s) 216, 755, 1065, 2807, 3226
GsaI CCCAGC 3 cut(s) 26, 958, 2006
GsuI CTGGAG 3 cut(s) 89, 2942, 3332
HaeIII GGCC 4 cut(s) 1449, 1878, 1994, 3287
HapII CCGG 3 cut(s) 2957, 3320, 3474
HhaI GCGC 2 cut(s) 802, 1092
Hin6I GCGC 2 cut(s) 800, 1090
HinP1I GCGC 2 cut(s) 800, 1090
HincII GTYRAC 2 cut(s) 2580, 2587
HindII GTYRAC 2 cut(s) 2580, 2587
HindIII AAGCTT 2 cut(s) 507, 2795
HpaI GTTAAC 2 cut(s) 2580, 2587
HpaII CCGG 3 cut(s) 2957, 3320, 3474
HphI GGTGA 4 cut(s) 1348, 1355, 2474, 3110
Hpy166II GTNNAC 5 cut(s) 1666, 2580, 2587, 2657, 2690
Hpy8I GTNNAC 5 cut(s) 1666, 2580, 2587, 2657, 2690
Hpy99I CGWCG 2 cut(s) 408, 1988
HpyCH4III ACNGT 9 cut(s) 352, 492, 1113, 1319, 1458, 1691, 2584, 2936, 3209
HpyCH4IV ACGT 3 cut(s) 2027, 2442, 2662
HpySE526I ACGT 3 cut(s) 2027, 2442, 2662
HspAI GCGC 2 cut(s) 800, 1090
KpnI GGTACC 1 cut(s) 2472
KroI GCCGGC 1 cut(s) 3319
KroNI GCCGGC 1 cut(s) 3321
KspAI GTTAAC 2 cut(s) 2580, 2587
LguI GCTCTTC 2 cut(s) 33, 153
Lsp1109I GCAGC 5 cut(s) 227, 741, 1076, 2793, 3212
MabI ACCWGGT 1 cut(s) 9
MaeI CTAG 8 cut(s) 270, 426, 1163, 1679, 2279, 2304, 2835, 3015
MaeII ACGT 3 cut(s) 2027, 2442, 2662
MaeIII GTNAC 8 cut(s) 444, 691, 868, 1796, 2053, 2487, 2930, 2936
MfeI CAATTG 1 cut(s) 1020
MflI RGATCY 4 cut(s) 1681, 2300, 2869, 3278
MhlI GDGCHC 2 cut(s) 1576, 2703
MlsI TGGCCA 1 cut(s) 3287
MluNI TGGCCA 1 cut(s) 3287
MlyI GAGTC 7 cut(s) 695, 899, 1909, 2117, 2314, 3161, 3263
MmeI TCCRAC 5 cut(s) 198, 762, 1128, 1525, 2544
Mox20I TGGCCA 1 cut(s) 3287
Mph1103I ATGCAT 1 cut(s) 529
MroNI GCCGGC 1 cut(s) 3319
MroXI GAANNNNTTC 5 cut(s) 729, 1521, 2238, 2628, 2715
MscI TGGCCA 1 cut(s) 3287
MslI CAYNNNNRTG 4 cut(s) 807, 1478, 1692, 2445
Msp20I TGGCCA 1 cut(s) 3287
MspI CCGG 3 cut(s) 2957, 3320, 3474
MspR9I CCNGG 4 cut(s) 11, 515, 2298, 2912
MunI CAATTG 1 cut(s) 1020
MvaI CCWGG 4 cut(s) 11, 515, 2298, 2912
NaeI GCCGGC 1 cut(s) 3321
NgoMIV GCCGGC 1 cut(s) 3319
NlaIV GGNNCC 2 cut(s) 313, 2470
NmuCI GTSAC 4 cut(s) 444, 2487, 2930, 2936
NsbI TGCGCA 1 cut(s) 801
NsiI ATGCAT 1 cut(s) 529
NspI RCATGY 1 cut(s) 1905
NspV TTCGAA 2 cut(s) 2989, 3133
OliI CACNNNNGTG 2 cut(s) 1692, 2445
PaeI GCATGC 1 cut(s) 1905
PaeR7I CTCGAG 3 cut(s) 146, 206, 3034
PagI TCATGA 3 cut(s) 669, 897, 2154
PceI AGGCCT 2 cut(s) 1878, 1994
PciSI GCTCTTC 2 cut(s) 33, 153
PdiI GCCGGC 1 cut(s) 3321
PdmI GAANNNNTTC 5 cut(s) 729, 1521, 2238, 2628, 2715
PflFI GACNNNGTC 1 cut(s) 11
PflMI CCANNNNNTGG 2 cut(s) 2384, 2859
PfoI TCCNGGA 1 cut(s) 2296
PkrI GCNGC 5 cut(s) 217, 756, 1066, 2808, 3227
PleI GAGTC 7 cut(s) 694, 899, 1909, 2116, 2314, 3161, 3263
PpsI GAGTC 7 cut(s) 694, 899, 1909, 2116, 2314, 3161, 3263
PsiI TTATAA 1 cut(s) 2285
Psp6I CCWGG 4 cut(s) 9, 513, 2296, 2910
PspFI CCCAGC 3 cut(s) 22, 954, 2002
PspGI CCWGG 4 cut(s) 9, 513, 2296, 2910
PspN4I GGNNCC 2 cut(s) 313, 2470
PspXI VCTCGAGB 1 cut(s) 3034
PstI CTGCAG 2 cut(s) 1815, 2341
PstNI CAGNNNCTG 2 cut(s) 1117, 2209
PsuI RGATCY 4 cut(s) 1681, 2300, 2869, 3278
PsyI GACNNNGTC 1 cut(s) 11
RsaI GTAC 4 cut(s) 1424, 2166, 2470, 3353
RsaNI GTAC 4 cut(s) 1423, 2165, 2469, 3352
RseI CAYNNNNRTG 4 cut(s) 807, 1478, 1692, 2445
SapI GCTCTTC 2 cut(s) 33, 153
SatI GCNGC 5 cut(s) 216, 755, 1065, 2807, 3226
SchI GAGTC 7 cut(s) 695, 899, 1909, 2117, 2314, 3161, 3263
ScrFI CCNGG 4 cut(s) 11, 515, 2298, 2912
SduI GDGCHC 2 cut(s) 1576, 2703
SexAI ACCWGGT 1 cut(s) 9
SfcI CTRYAG 2 cut(s) 1811, 2337
Sfr274I CTCGAG 3 cut(s) 146, 206, 3034
SfuI TTCGAA 2 cut(s) 2989, 3133
SlaI CTCGAG 3 cut(s) 146, 206, 3034
SmiMI CAYNNNNRTG 4 cut(s) 807, 1478, 1692, 2445
SmlI CTYRAG 7 cut(s) 92, 146, 206, 504, 1952, 3034, 3380
SmoI CTYRAG 7 cut(s) 92, 146, 206, 504, 1952, 3034, 3380
SpeI ACTAGT 2 cut(s) 2834, 3014
SphI GCATGC 1 cut(s) 1905
SseBI AGGCCT 2 cut(s) 1878, 1994
SsiI CCGC 6 cut(s) 155, 877, 891, 2258, 2647, 3495
SspI AATATT 2 cut(s) 1242, 2716
SspMI CTAG 8 cut(s) 270, 426, 1163, 1679, 2279, 2304, 2835, 3015
StuI AGGCCT 2 cut(s) 1878, 1994
StyD4I CCNGG 4 cut(s) 9, 513, 2296, 2910
StyI CCWWGG 3 cut(s) 2058, 3158, 3282
TaaI ACNGT 9 cut(s) 352, 492, 1113, 1319, 1458, 1691, 2584, 2936, 3209
TaiI ACGT 3 cut(s) 2030, 2445, 2665
TscAI CASTG 4 cut(s) 1118, 2925, 3207, 3212
TseFI GTSAC 4 cut(s) 444, 2487, 2930, 2936
TseI GCWGC 5 cut(s) 215, 754, 1064, 2806, 3225
Tsp45I GTSAC 4 cut(s) 444, 2487, 2930, 2936
TspGWI ACGGA 1 cut(s) 249
TspRI CASTG 4 cut(s) 1118, 2925, 3207, 3212
Tth111I GACNNNGTC 1 cut(s) 11
Van91I CCANNNNNTGG 2 cut(s) 2384, 2859
XapI RAATTY 7 cut(s) 189, 913, 1259, 1521, 2218, 2238, 2680
XbaI TCTAGA 1 cut(s) 2303
XceI RCATGY 1 cut(s) 1905
XhoI CTCGAG 3 cut(s) 146, 206, 3034
XmiI GTMKAC 1 cut(s) 1665
XmnI GAANNNNTTC 5 cut(s) 729, 1521, 2238, 2628, 2715
XspI CTAG 8 cut(s) 270, 426, 1163, 1679, 2279, 2304, 2835, 3015
Zsp2I ATGCAT 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.