Rh7BG405000

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000400
Physical Location & Seq
Reverse (-)
45319969 .. 45320724
756 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG405000.1

Sequence Viewer

Length: 651 bp
ATGCTGAACTTCAGTGGATATGGAATTAAGAGGCTCCACCGAGAGATTGGTGGGCTAATATCTTTGCGGTATCTTTATCTTTCAAATACTCTCCTAGATACGATGCCAGCGACTATCTGTGATCTTTGCAATCTGCAGACACTGAATCTCTCAAGTTGTAGTGTGCTCAAGGAGTTACCAAGAGGTACTACCAAGTTGAGAAATCTGAGACATCTTAACATAGATGATTGTCCAAGACTTGCTGGCATGCCTCCATCAATGGGAATTTTACAACAACTTCAAACTTTGCCAGTATATATCGTCGGCCGCAACTTTGAAACTTCTATTTTTCAGCTCTCATCAATGAATCTGCGAGGGAAGTTAAAAATCAAATGTCTGGAGGAGGCTAAAATTCCATTTGGGAACAACATGATTAAAGTGTGGATGAAAACTAAAGAGTTTTATTCATTGGAACTGTTGTGGCAAAATGATGATGGGGGCAAGCTAGATCAAAACAGATCTAGGCAAGCTGGCAGGCAAGTTGATGATAAAACTGATTTTACTCTGGTAGATTCTTTAACTGTATCTCCATTTGTAAGGATGTTGTCAATAAATGGTTATTCAGGAACAAAGTTCCCAGATGAGATGAGTTGGTCTCAGAAACTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

216

Amino Acids

24.6

Weight (kDa)

9.1

Isoelectric Point (pI)

43.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 14 - 156 1.4e-18 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 111 - 209 1.8e-08 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 67, 307
AcoI YGGCCR 1 cut(s) 304
AcsI RAATTY 2 cut(s) 264, 390
AfaI GTAC 1 cut(s) 187
AfiI CCNNNNNNNGG 1 cut(s) 260
AgsI TTSAA 3 cut(s) 84, 281, 317
AluBI AGCT 3 cut(s) 334, 484, 509
AluI AGCT 3 cut(s) 334, 484, 509
Alw21I GWGCWC 1 cut(s) 168
Alw26I GTCTC 2 cut(s) 202, 639
AlwNI CAGNNNCTG 1 cut(s) 142
AoxI GGCC 1 cut(s) 304
ApoI RAATTY 2 cut(s) 264, 390
Bbv12I GWGCWC 1 cut(s) 168
BccI CCATC 2 cut(s) 262, 467
BcoDI GTCTC 2 cut(s) 202, 639
BfaI CTAG 3 cut(s) 95, 485, 501
BfmI CTRYAG 1 cut(s) 134
BglII AGATCT 1 cut(s) 497
BisI GCNGC 1 cut(s) 307
BlsI GCNGC 1 cut(s) 308
BmiI GGNNCC 1 cut(s) 35
BmsI GCATC 1 cut(s) 93
BplI GAGNNNNNCTC 2 cut(s) 619, 651
BpmI CTGGAG 1 cut(s) 398
BpuEI CTTGAG 2 cut(s) 136, 152
BsaI GGTCTC 1 cut(s) 639
BsaXI ACNNNNNCTCC 2 cut(s) 550, 580
Bsc4I CCNNNNNNNGG 1 cut(s) 260
Bse1I ACTGG 1 cut(s) 290
BseGI GGATG 2 cut(s) 429, 585
BseLI CCNNNNNNNGG 1 cut(s) 260
BseMII CTCAG 2 cut(s) 197, 650
BseNI ACTGG 1 cut(s) 290
BseRI GAGGAG 1 cut(s) 395
BseX3I CGGCCG 1 cut(s) 304
Bsh1285I CGRYCG 1 cut(s) 307
BshFI GGCC 1 cut(s) 306
BsiEI CGRYCG 1 cut(s) 307
BsiHKAI GWGCWC 1 cut(s) 168
BslI CCNNNNNNNGG 1 cut(s) 260
BsmAI GTCTC 2 cut(s) 202, 639
BsnI GGCC 1 cut(s) 306
Bso31I GGTCTC 1 cut(s) 639
Bsp1286I GDGCHC 1 cut(s) 168
Bsp143I GATC 3 cut(s) 121, 487, 497
BspACI CCGC 2 cut(s) 67, 307
BspANI GGCC 1 cut(s) 306
BspCNI CTCAG 2 cut(s) 198, 649
BspLI GGNNCC 1 cut(s) 35
BspMAI CTGCAG 1 cut(s) 138
BspTNI GGTCTC 1 cut(s) 639
BsrI ACTGG 1 cut(s) 290
BssMI GATC 3 cut(s) 121, 487, 497
Bst4CI ACNGT 2 cut(s) 456, 562
BstC8I GCNNGC 7 cut(s) 108, 244, 248, 482, 507, 511, 515
BstDEI CTNAG 2 cut(s) 206, 636
BstF5I GGATG 2 cut(s) 429, 585
BstKTI GATC 3 cut(s) 124, 490, 500
BstMAI GTCTC 2 cut(s) 202, 639
BstMBI GATC 3 cut(s) 121, 487, 497
BstMCI CGRYCG 1 cut(s) 307
BstNSI RCATGY 1 cut(s) 250
BstSFI CTRYAG 1 cut(s) 134
BstX2I RGATCY 1 cut(s) 497
BstYI RGATCY 1 cut(s) 497
BstZI CGGCCG 1 cut(s) 304
BsuRI GGCC 1 cut(s) 306
BtsCI GGATG 2 cut(s) 429, 585
BtsIMutI CAGTG 2 cut(s) 19, 140
Cac8I GCNNGC 7 cut(s) 108, 244, 248, 482, 507, 511, 515
CaiI CAGNNNCTG 1 cut(s) 142
Csp6I GTAC 1 cut(s) 186
CviAII CATG 2 cut(s) 247, 409
CviJI RGCY 7 cut(s) 34, 55, 306, 334, 386, 484, 509
CviKI_1 RGCY 7 cut(s) 34, 55, 306, 334, 386, 484, 509
CviQI GTAC 1 cut(s) 186
DdeI CTNAG 2 cut(s) 206, 636
DpnI GATC 3 cut(s) 123, 489, 499
DpnII GATC 3 cut(s) 121, 487, 497
EaeI YGGCCR 1 cut(s) 304
EagI CGGCCG 1 cut(s) 304
EclXI CGGCCG 1 cut(s) 304
Eco31I GGTCTC 1 cut(s) 639
Eco52I CGGCCG 1 cut(s) 304
FaeI CATG 2 cut(s) 250, 412
FaiI YATR 6 cut(s) 21, 221, 248, 295, 297, 410
FatI CATG 2 cut(s) 246, 408
Fnu4HI GCNGC 1 cut(s) 307
FokI GGATG 2 cut(s) 436, 592
Fsp4HI GCNGC 1 cut(s) 307
FspBI CTAG 3 cut(s) 95, 485, 501
GluI GCNGC 1 cut(s) 307
GsuI CTGGAG 1 cut(s) 398
HaeIII GGCC 1 cut(s) 306
Hin1II CATG 2 cut(s) 250, 412
HinfI GANTC 3 cut(s) 145, 346, 551
Hpy188I TCNGA 2 cut(s) 207, 639
Hpy188III TCNNGA 2 cut(s) 377, 603
Hpy99I CGWCG 1 cut(s) 305
HpyCH4III ACNGT 2 cut(s) 456, 562
HpyCH4V TGCA 2 cut(s) 129, 136
HpyF3I CTNAG 2 cut(s) 206, 636
Hsp92II CATG 2 cut(s) 250, 412
Kzo9I GATC 3 cut(s) 121, 487, 497
LmnI GCTCC 1 cut(s) 39
LpnPI CCDG 9 cut(s) 120, 228, 303, 362, 495, 499, 530, 588, 630
LweI GCATC 1 cut(s) 93
MaeI CTAG 3 cut(s) 95, 485, 501
MaeIII GTNAC 1 cut(s) 174
MalI GATC 3 cut(s) 123, 489, 499
MboI GATC 3 cut(s) 121, 487, 497
MflI RGATCY 1 cut(s) 497
MhlI GDGCHC 1 cut(s) 168
MluCI AATT 3 cut(s) 24, 264, 390
MnlI CCTC 6 cut(s) 24, 176, 261, 347, 373, 376
MseI TTAA 5 cut(s) 27, 216, 362, 414, 557
NdeII GATC 3 cut(s) 121, 487, 497
NlaIII CATG 2 cut(s) 250, 412
NlaIV GGNNCC 1 cut(s) 35
NspI RCATGY 1 cut(s) 250
PaeI GCATGC 1 cut(s) 250
PcsI WCGNNNNNNNCGW 1 cut(s) 107
PfeI GAWTC 3 cut(s) 145, 346, 551
PkrI GCNGC 1 cut(s) 308
PspN4I GGNNCC 1 cut(s) 35
PstI CTGCAG 1 cut(s) 138
PstNI CAGNNNCTG 1 cut(s) 142
PsuI RGATCY 1 cut(s) 497
RsaI GTAC 1 cut(s) 187
RsaNI GTAC 1 cut(s) 186
SaqAI TTAA 5 cut(s) 27, 216, 362, 414, 557
SatI GCNGC 1 cut(s) 307
Sau3AI GATC 3 cut(s) 121, 487, 497
SduI GDGCHC 1 cut(s) 168
SetI ASST 4 cut(s) 187, 336, 486, 511
SfaNI GCATC 1 cut(s) 93
SfcI CTRYAG 1 cut(s) 134
SmlI CTYRAG 2 cut(s) 151, 167
SmoI CTYRAG 2 cut(s) 151, 167
SphI GCATGC 1 cut(s) 250
Sse9I AATT 3 cut(s) 24, 264, 390
SsiI CCGC 2 cut(s) 67, 307
SspMI CTAG 3 cut(s) 95, 485, 501
TaaI ACNGT 2 cut(s) 456, 562
TasI AATT 3 cut(s) 24, 264, 390
TauI GCSGC 1 cut(s) 309
TfiI GAWTC 3 cut(s) 145, 346, 551
Tru1I TTAA 5 cut(s) 27, 216, 362, 414, 557
Tru9I TTAA 5 cut(s) 27, 216, 362, 414, 557
TscAI CASTG 2 cut(s) 19, 147
TspDTI ATGAA 3 cut(s) 359, 435, 440
TspRI CASTG 2 cut(s) 19, 147
XapI RAATTY 2 cut(s) 264, 390
XceI RCATGY 1 cut(s) 250
XcmI CCANNNNNNNNNTGG 1 cut(s) 44
XspI CTAG 3 cut(s) 95, 485, 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.