RchiOBHm_Chr1g0348561

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000400
Physical Location & Seq
Reverse (-)
41469677 .. 41473207
3531 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57458

Sequence Viewer

Length: 3531 bp
ATGGCAGACGTAGTTTTATCACCAGCTTTGCAAGTGATCTTTGACAGAGTAGCATCCCCAGTCCTACAAAAGCTTGCTGATATCTGGGACATGAAGGACAACCTCCAGAGCCTACAACATGCCTTGGTCATGGTTCAAGCTATTCTTGAAGATGCAGAGGAGCAACAACTGACCAATAAAGCCGTCCGGCTTTGGCTGTTGAAGCTCAAGAACGCAGCTCATGATGCTGATGACCTTCTGGATTTCGTTACGGCCAAAGGAAAAGATAAGTGGACTTTAGCAATTTCAAAATCCATAGATGCTGACAAAGTCAGAAAGATGCTTTTGACATTAGAAATGACAGTAAAAGAGGGTCTATTGAAGTTTAATTTTCGAGAGCCTGGTACAGTAGATAGGCAATCTGATAAAAGGGAGACTAGCTCTTTTGTTATGGAGTCAGAAATATATGGAAGAGCTGATGACAAAGAGAACTTAGTGAAACTCTTACTATCTTCTGAGTGTCACCAGGATGGTTATGCTATGTGTATTCCGATAATTGGTATTGGAGGAATTGGCAAGACGACTCTTGCTCAGTTGGCATATAATGATGAGCGGGTAATCCAACATTTTGATGTTAGGCTGTGGACTTTTGTCTCGCATGATTTCAATATCAAGAAGATCATGAAGTCAATTATTGAGTCTGTAACAAGGGAAGATTGCAAGTTCTCGGAGAGTGACTTACTTCAATCTCGACTTTGGCATTTGCTGCACAACAAAAGATATCTAATTGTGTTGGATGATGTTTGGACTGAAGATCAAGATGACTGGGACAAACTAAGACCTTTGTTTAGACGAGGTGTTGATGGATGCAAAATCATTATCACTTCCCGCAATAAAAAAATCCCATTCATGATGGACTCCCTAAATTCGGCATACTATTTAAAGGGTTTGATGGAGGATGACTGCTGGGCTTTGTTCAAGCAACGGGCTTTTGGACGAGAAGAAGAAGAGAAGTATCCCGACCTCTGCCTGATTGGAAAGCAGATTGCAAAGACTTGTGGGGGTGTACCATTAGCAGCCAAAAGTTTGGGAAGTGTTATGCGCTTTAAAAGAGAAGAACGGCAATGGTTGTTTATGCAAAATAGTGAACTTTGGGAAGTAGATGGATGCAAACAGAGAATTTTACCTGCGCTTATGTTAAGTTATCTTCATCTGGCAGCACATCTTCGACAATGCTTTGCATTCTGCTCCATATTTCCTAAAAATTATGAATTCAAGAAGCATAAATTAGTTCTCTTGTGGATGGCAGAGGGCTTAATTCTAGAAGAAGGTAGCAAGCGACCAGAAGACATTGGTGATGAATACTTTTCTGATTTGCTGTGGATGTCTTTCTTTCAAGAAGTAGAGTTACGTGATGGTGGTAGCACAATTGGATACAAAATGAATGATGTCATTCATGATCTTGCACGATATGTTTCTGGAAATGAATCCGTTATGCTTGAACAAGGTCTTCCACCAAGTAATCCAGCACAAATTCGTCGCTCATCCATTATTTATAAATACAGGGAAGTTACAATTCCAGAAGCTCTCTTTGAAGCAGAACATTTACGAACTGTCTTATTGATTGGAGAATATGGACTTCTAAAAGACAGTAGTAAAATGTTATTAAATTGTATATACCTGCGTGTGCTGGACTTGAACAACTGTGATGTTCATTTTTTGCCGTCATCAATAGGTGGGTTGATATGTTTGAGGTATCTCGACTTGTCTTATACGCCTATCTCCAAGTTGCCGGCAACCACAAGGAATCTCTGTTCATTGCAGACTTTAAACCTCTCTGGATGTCATAATCTTAGAGTATTGCGAGACTTCGAAGCAATGACCAGCCTAAGACATCTCAATATAACTGGATGTGATAGCTTGTGGTATATATCTCCTTGTATTAAAATGTTACTTCAACTTCAGACACTGCCATTATATTTCGTTCGCAAGAATCCCGGTAATCTTGGTGCACTAGAGAATTTGAATCTTTATGGGGAGTTGAATATTACACACTTGGAGAATATAAGAAATGCAACTGGAGCTCAATCATCTGGACTGAAGATGAAAGAAAATATTGAGTCGTTGGGATTATACTGGGGAGATTTTGGAGATGTGCACGATAGGAATAAATCAATATTGAAGAAACCAGCCAAGTCCTCAGCCAAATGGTACTGTTCAGAAGTAGGGATGCGGTGGCATGAACCTGATGCTGAAGAGGCGGTAGAAATTCTTGATGGCCTGAAACCACACAAGAATTTGAAAAAGCTAGTTATACATGGTTATCCAGGAATCAGATTTCCAGATTGGAAACTGCCAAATCTAATTGCAGTTGATTTGTCCAATTGTAAGAACTGTGAATATCTTCCAGCCCTTGGGAATCTTCTTCTACTCAAGACTCTTTCTCTGCATGGAATGGAGAGCGTGACGCGCATTGGCTTTGAGTTCCATCGTGATAGTACAGACATATGCTTTTCATCACTTGAAGAACTATCGCTCAGTGATTTTCCGAAATTGGAAACCTGGTCGAGTGCAAATAATGAAAATGCATTTCCGAGCTTGAGGAAGTTAACCATACGTAGGTGTCCCAAGTTGACACAGATACCATTTTGTTTGTCTCTTCAACATTTGGAGCTGCGGGATTGTAATCCATCTTCAATCTTCATTGGAGGTTTAACTTTGCTGACAGTGCTGGTAATAGAAAAACTTCCACAGCTGCGCACTCTACCACAAGGGCTTTCTGCATCAGCTAGTCTGTCTTCTCTGGAGGTCTTATCTTGCCCCAAGCTTTGTTCACTGCCTTTGGAGATGGGAAACCTCACTGCACTGAAATCATTGACCATTCGTTGGTGTGATGAGTTGTCCTCTCTGCCACAGAGTTTGCAGAACCTCAAAGCTCTGGAATCACTAGAGATTAGTGACTGTCATAGTATAATCTCGATGCCAGATGGTGCAATTGGAGGTTTGAGTTCCCTTCAAACTTTGTCCATTGAGAACTGCAGTAGTCTGACTTCTTTGTCTTCGAGTCTGGAACAACTCGCTTTCCTTGAGCGGTTCACCATTATGTACTGTCCAAACCTTGGTTCTTTCCCAGAGGGTGTGCAACACCTCTCAACCCTTCGAACTTTGTCTATCTTGAGCTGTCCTTGGTTTGATTCTTTGCCAGAAGAGTTGCAAATGGTCAAGACGCTGCAGTGTCTGGAAATTAGTAGCTGCCCGAATTTCACAGCTTTGCCAGAGTGGGTTGAGAAGCTTGCTTCACTCAGATCTTTGACCATTTTTGATTGTCCTAATTTGAAACTGTTGCCACAAAGTCTAGAACTTCTCACTAAACTCCAGCACCTCTCTATTCAAGAATGTCCCGAGCTTGAGGAAAGATGTAGACAAGGCAGTGGTGAGGATTGGCTGAAAATAGCTCATGTTCCACATAAGCACATTGGATCACCGGAGATGAGGCAGTCCAGTAGTGAAGCAAGTACATCGGGCAGCTCTTCTGTTGAAACATCTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1176

Amino Acids

133.11

Weight (kDa)

5.92

Isoelectric Point (pI)

54.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 85 1.1e-16 Rx N-terminal domain
NB-ARC PF00931 154 - 327 1.1e-36 NB-ARC domain
WHD_DRP PF23559 411 - 482 1.2e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 547 - 883 5.1e-18 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 663 - 812 1.3e-26 R13L1/DRL21 LRRs
LRR_14 PF23598 880 - 1022 7.6e-08 Leucine-rich repeat region
LRR_14 PF23598 1002 - 1125 1.5e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1536
AasI GACNNNNNNGTC 1 cut(s) 3034
Acc16I TGCGCA 1 cut(s) 2738
Acc36I ACCTGC 2 cut(s) 1174, 1668
AccB7I CCANNNNNTGG 3 cut(s) 2393, 2865, 3098
AccBSI CCGCTC 2 cut(s) 592, 3070
AccI GTMKAC 1 cut(s) 3400
AccII CGCG 1 cut(s) 2449
AciI CCGC 6 cut(s) 592, 868, 2212, 2240, 2656, 3070
AclWI GGATC 1 cut(s) 3466
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 8 cut(s) 904, 1158, 1250, 1512, 1999, 2247, 2275, 3238
AcuI CTGAAG 4 cut(s) 810, 1925, 2099, 2253
AfaI GTAC 6 cut(s) 385, 1047, 2192, 2479, 3086, 3496
AfiI CCNNNNNNNGG 6 cut(s) 536, 907, 2393, 2598, 2865, 3098
AjnI CCWGG 4 cut(s) 379, 504, 2305, 2540
AjuI GAANNNNNNNTTGG 2 cut(s) 3085, 3117
AloI GAACNNNNNNTCC 2 cut(s) 1777, 1809
Alw21I GWGCWC 3 cut(s) 1993, 2065, 2139
Alw26I GTCTC 4 cut(s) 407, 637, 1839, 2640
Alw44I GTGCAC 2 cut(s) 1989, 2135
AlwI GGATC 1 cut(s) 3466
AlwNI CAGNNNCTG 2 cut(s) 1948, 3217
Ama87I CYCGRG 1 cut(s) 3380
AoxI GGCC 2 cut(s) 252, 2257
ApaLI GTGCAC 2 cut(s) 1989, 2135
ApeKI GCWGC 9 cut(s) 215, 745, 1055, 1196, 2653, 2734, 3208, 3231, 3504
ApoI RAATTY 8 cut(s) 904, 1158, 1250, 1512, 1999, 2247, 2275, 3238
Asp700I GAANNNNTTC 2 cut(s) 2382, 2724
AspLEI GCGC 4 cut(s) 1083, 1171, 2451, 2739
AsuC2I CCSGG 1 cut(s) 1977
AsuHPI GGTGA 6 cut(s) 12, 494, 1346, 3067, 3425, 3453
AsuII TTCGAA 2 cut(s) 1851, 3139
AvaI CYCGRG 1 cut(s) 3380
BaeGI GKGCMC 2 cut(s) 1993, 2139
BanII GRGCYC 1 cut(s) 2065
BarI GAAGNNNNNNTAC 6 cut(s) 1371, 1403, 2656, 2688, 3013, 3045
BbsI GAAGAC 4 cut(s) 1332, 1481, 2769, 3030
Bbv12I GWGCWC 3 cut(s) 1993, 2065, 2139
BbvCI CCTCAGC 1 cut(s) 2179
BbvI GCAGC 9 cut(s) 227, 732, 1067, 1208, 2640, 2721, 3195, 3218, 3516
BceAI ACGGC 4 cut(s) 167, 267, 1115, 1687
BcgI CGANNNNNNTGC 2 cut(s) 3480, 3514
BciT130I CCWGG 4 cut(s) 381, 506, 2307, 2542
BciVI GTATCC 2 cut(s) 1005, 1406
BcnI CCSGG 1 cut(s) 1977
BcoDI GTCTC 4 cut(s) 407, 637, 1839, 2640
BfaI CTAG 7 cut(s) 417, 1301, 1994, 2288, 2769, 2927, 3335
BfmI CTRYAG 2 cut(s) 3016, 3209
BfuAI ACCTGC 2 cut(s) 1174, 1668
BfuI GTATCC 2 cut(s) 1005, 1406
BglII AGATCT 1 cut(s) 3284
BisI GCNGC 9 cut(s) 216, 746, 1056, 1197, 2654, 2735, 3209, 3232, 3505
BlsI GCNGC 9 cut(s) 217, 747, 1057, 1198, 2655, 2736, 3210, 3233, 3506
Bme1390I CCNGG 5 cut(s) 381, 506, 1977, 2307, 2542
BmeT110I CYCGRG 1 cut(s) 3380
BmrFI CCNGG 5 cut(s) 381, 506, 1977, 2307, 2542
BmrI ACTGGG 3 cut(s) 53, 814, 2125
BmuI ACTGGG 3 cut(s) 53, 814, 2125
BoxI GACNNNNGTC 1 cut(s) 629
BpiI GAAGAC 4 cut(s) 1332, 1481, 2769, 3030
BplI GAGNNNNNCTC 4 cut(s) 404, 436, 2867, 2899
BpmI CTGGAG 4 cut(s) 89, 2079, 2804, 3338
Bpu10I CCTNAGC 1 cut(s) 2179
Bpu14I TTCGAA 2 cut(s) 1851, 3139
BpuEI CTTGAG 6 cut(s) 191, 2396, 2599, 3086, 3175, 3407
BpuMI CCSGG 1 cut(s) 1977
BsaAI YACGTR 2 cut(s) 1391, 2597
BsaBI GATNNNNATC 1 cut(s) 2664
BsaJI CCNNGG 4 cut(s) 123, 2392, 3097, 3164
BsaWI WCCGGW 1 cut(s) 3463
Bsc4I CCNNNNNNNGG 6 cut(s) 536, 907, 2393, 2598, 2865, 3098
Bse118I RCCGGY 1 cut(s) 1771
Bse1I ACTGG 6 cut(s) 59, 809, 1891, 2062, 2120, 3480
Bse3DI GCAATG 3 cut(s) 1109, 1796, 1863
Bse8I GATNNNNATC 1 cut(s) 2664
BseBI CCWGG 4 cut(s) 381, 506, 2307, 2542
BseDI CCNNGG 4 cut(s) 123, 2392, 3097, 3164
BseJI GATNNNNATC 1 cut(s) 2664
BseLI CCNNNNNNNGG 6 cut(s) 536, 907, 2393, 2598, 2865, 3098
BseMI GCAATG 3 cut(s) 1109, 1796, 1863
BseMII CTCAG 5 cut(s) 486, 584, 2193, 2530, 3295
BseNI ACTGG 6 cut(s) 59, 809, 1891, 2062, 2120, 3480
BseRI GAGGAG 1 cut(s) 173
BseSI GKGCMC 2 cut(s) 1993, 2139
BseXI GCAGC 9 cut(s) 227, 732, 1067, 1208, 2640, 2721, 3195, 3218, 3516
BseYI CCCAGC 1 cut(s) 945
BsgI GTGCAG 2 cut(s) 731, 2826
Bsh1236I CGCG 1 cut(s) 2449
BshFI GGCC 2 cut(s) 254, 2259
BsiHKAI GWGCWC 3 cut(s) 1993, 2065, 2139
BsiHKCI CYCGRG 1 cut(s) 3380
BsiSI CCGG 4 cut(s) 187, 1772, 1977, 3464
BslFI GGGAC 4 cut(s) 101, 821, 2589, 3363
BslI CCNNNNNNNGG 6 cut(s) 536, 907, 2393, 2598, 2865, 3098
BsmAI GTCTC 4 cut(s) 407, 637, 1839, 2640
BsmFI GGGAC 4 cut(s) 101, 821, 2589, 3363
BsmI GAATGC 1 cut(s) 1220
BsnI GGCC 2 cut(s) 254, 2259
BsoBI CYCGRG 1 cut(s) 3380
Bsp119I TTCGAA 2 cut(s) 1851, 3139
Bsp1286I GDGCHC 3 cut(s) 1993, 2065, 2139
Bsp143I GATC 6 cut(s) 36, 657, 793, 1438, 3284, 3458
BspACI CCGC 6 cut(s) 592, 868, 2212, 2240, 2656, 3070
BspANI GGCC 2 cut(s) 254, 2259
BspCNI CTCAG 5 cut(s) 487, 583, 2192, 2529, 3294
BspFNI CGCG 1 cut(s) 2449
BspHI TCATGA 4 cut(s) 220, 660, 888, 1435
BspMAI CTGCAG 2 cut(s) 3020, 3213
BspMI ACCTGC 2 cut(s) 1174, 1668
BspPI GGATC 1 cut(s) 3466
BspQI GCTCTTC 2 cut(s) 445, 3514
BspT104I TTCGAA 2 cut(s) 1851, 3139
BsrBI CCGCTC 2 cut(s) 592, 3070
BsrDI GCAATG 3 cut(s) 1109, 1796, 1863
BsrFI RCCGGY 1 cut(s) 1771
BsrI ACTGG 6 cut(s) 59, 809, 1891, 2062, 2120, 3480
BssAI RCCGGY 1 cut(s) 1771
BssECI CCNNGG 4 cut(s) 123, 2392, 3097, 3164
BssMI GATC 6 cut(s) 36, 657, 793, 1438, 3284, 3458
BssT1I CCWWGG 4 cut(s) 123, 2392, 3097, 3164
Bst2UI CCWGG 4 cut(s) 381, 506, 2307, 2542
Bst6I CTCTTC 6 cut(s) 445, 981, 2229, 2643, 3180, 3514
BstAPI GCANNNNNTGC 1 cut(s) 745
BstBAI YACGTR 2 cut(s) 1391, 2597
BstBI TTCGAA 2 cut(s) 1851, 3139
BstC8I GCNNGC 4 cut(s) 75, 1316, 1773, 3273
BstDEI CTNAG 9 cut(s) 472, 495, 570, 815, 1832, 1868, 2179, 2516, 3281
BstFNI CGCG 1 cut(s) 2449
BstHHI GCGC 4 cut(s) 1083, 1171, 2451, 2739
BstKTI GATC 6 cut(s) 39, 660, 796, 1441, 3287, 3461
BstMAI GTCTC 4 cut(s) 407, 637, 1839, 2640
BstMBI GATC 6 cut(s) 36, 657, 793, 1438, 3284, 3458
BstMWI GCNNNNNNNGC 8 cut(s) 202, 224, 575, 745, 2060, 2237, 2448, 2707
BstNI CCWGG 4 cut(s) 381, 506, 2307, 2542
BstNSI RCATGY 1 cut(s) 122
BstPAI GACNNNNGTC 1 cut(s) 629
BstSCI CCNGG 5 cut(s) 379, 504, 1975, 2305, 2540
BstSFI CTRYAG 2 cut(s) 3016, 3209
BstSLI GKGCMC 2 cut(s) 1993, 2139
BstSNI TACGTA 1 cut(s) 2597
BstUI CGCG 1 cut(s) 2449
BstV1I GCAGC 9 cut(s) 227, 732, 1067, 1208, 2640, 2721, 3195, 3218, 3516
BstV2I GAAGAC 4 cut(s) 1332, 1481, 2769, 3030
BstX2I RGATCY 1 cut(s) 3284
BstXI CCANNNNNNTGG 1 cut(s) 1066
BstYI RGATCY 1 cut(s) 3284
BsuI GTATCC 2 cut(s) 1005, 1406
BsuRI GGCC 2 cut(s) 254, 2259
BtsI GCAGTG 5 cut(s) 1946, 2813, 2838, 3218, 3415
BtsIMutI CAGTG 8 cut(s) 1946, 2524, 2712, 2813, 2838, 2843, 3218, 3415
BveI ACCTGC 2 cut(s) 1174, 1668
Cac8I GCNNGC 4 cut(s) 75, 1316, 1773, 3273
CaiI CAGNNNCTG 2 cut(s) 1948, 3217
CciI TCATGA 4 cut(s) 220, 660, 888, 1435
CfoI GCGC 4 cut(s) 1083, 1171, 2451, 2739
Cfr10I RCCGGY 1 cut(s) 1771
CseI GACGC 2 cut(s) 2455, 3214
CsiI ACCWGGT 1 cut(s) 2540
Csp6I GTAC 6 cut(s) 384, 1046, 2191, 2478, 3085, 3495
CspCI CAANNNNNGTGG 2 cut(s) 2880, 2915
CviQI GTAC 6 cut(s) 384, 1046, 2191, 2478, 3085, 3495
DdeI CTNAG 9 cut(s) 472, 495, 570, 815, 1832, 1868, 2179, 2516, 3281
DpnI GATC 6 cut(s) 38, 659, 795, 1440, 3286, 3460
DpnII GATC 6 cut(s) 36, 657, 793, 1438, 3284, 3458
DraI TTTAAA 3 cut(s) 921, 1087, 1809
DrdI GACNNNNNNGTC 1 cut(s) 3034
DseDI GACNNNNNNGTC 1 cut(s) 3034
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 6 cut(s) 445, 981, 2229, 2643, 3180, 3514
EarI CTCTTC 6 cut(s) 445, 981, 2229, 2643, 3180, 3514
Ecl136II GAGCTC 1 cut(s) 2063
Eco105I TACGTA 1 cut(s) 2597
Eco130I CCWWGG 4 cut(s) 123, 2392, 3097, 3164
Eco24I GRGCYC 1 cut(s) 2065
Eco32I GATATC 2 cut(s) 82, 761
Eco53kI GAGCTC 1 cut(s) 2063
Eco57I CTGAAG 4 cut(s) 810, 1925, 2099, 2253
Eco88I CYCGRG 1 cut(s) 3380
EcoICRI GAGCTC 1 cut(s) 2063
EcoRI GAATTC 1 cut(s) 1250
EcoRII CCWGG 4 cut(s) 379, 504, 2305, 2540
EcoRV GATATC 2 cut(s) 82, 761
EcoT14I CCWWGG 4 cut(s) 123, 2392, 3097, 3164
EcoT22I ATGCAT 1 cut(s) 2569
EcoT38I GRGCYC 1 cut(s) 2065
ErhI CCWWGG 4 cut(s) 123, 2392, 3097, 3164
FalI AAGNNNNNCTT 2 cut(s) 129, 161
FaqI GGGAC 4 cut(s) 101, 821, 2589, 3363
FauI CCCGC 3 cut(s) 585, 875, 2649
FauNDI CATATG 1 cut(s) 2486
FblI GTMKAC 1 cut(s) 3400
Fnu4HI GCNGC 9 cut(s) 216, 746, 1056, 1197, 2654, 2735, 3209, 3232, 3505
FriOI GRGCYC 1 cut(s) 2065
Fsp4HI GCNGC 9 cut(s) 216, 746, 1056, 1197, 2654, 2735, 3209, 3232, 3505
FspBI CTAG 7 cut(s) 417, 1301, 1994, 2288, 2769, 2927, 3335
FspI TGCGCA 1 cut(s) 2738
GlaI GCGC 4 cut(s) 1082, 1170, 2450, 2738
GluI GCNGC 9 cut(s) 216, 746, 1056, 1197, 2654, 2735, 3209, 3232, 3505
GsaI CCCAGC 1 cut(s) 949
GsuI CTGGAG 4 cut(s) 89, 2079, 2804, 3338
HaeIII GGCC 2 cut(s) 254, 2259
HapII CCGG 4 cut(s) 187, 1772, 1977, 3464
HgaI GACGC 2 cut(s) 2455, 3214
HhaI GCGC 4 cut(s) 1083, 1171, 2451, 2739
Hin6I GCGC 4 cut(s) 1081, 1169, 2449, 2737
HinP1I GCGC 4 cut(s) 1081, 1169, 2449, 2737
HincII GTYRAC 2 cut(s) 2589, 2613
HindII GTYRAC 2 cut(s) 2589, 2613
HindIII AAGCTT 3 cut(s) 71, 2804, 3269
HpaI GTTAAC 1 cut(s) 2589
HpaII CCGG 4 cut(s) 187, 1772, 1977, 3464
HphI GGTGA 6 cut(s) 12, 494, 1346, 3067, 3425, 3453
Hpy99I CGWCG 1 cut(s) 1521
HpyAV CCTTC 5 cut(s) 88, 245, 1301, 3002, 3146
HpyCH4IV ACGT 3 cut(s) 9, 1390, 2596
HpyF10VI GCNNNNNNNGC 8 cut(s) 202, 224, 575, 745, 2060, 2237, 2448, 2707
HpyF3I CTNAG 9 cut(s) 472, 495, 570, 815, 1832, 1868, 2179, 2516, 3281
HpySE526I ACGT 3 cut(s) 9, 1390, 2596
HspAI GCGC 4 cut(s) 1081, 1169, 2449, 2737
KroI GCCGGC 1 cut(s) 1771
KroNI GCCGGC 1 cut(s) 1773
KspAI GTTAAC 1 cut(s) 2589
Kzo9I GATC 6 cut(s) 36, 657, 793, 1438, 3284, 3458
LguI GCTCTTC 2 cut(s) 445, 3514
LmnI GCTCC 4 cut(s) 160, 1232, 2060, 2650
Lsp1109I GCAGC 9 cut(s) 227, 732, 1067, 1208, 2640, 2721, 3195, 3218, 3516
MabI ACCWGGT 1 cut(s) 2540
MaeI CTAG 7 cut(s) 417, 1301, 1994, 2288, 2769, 2927, 3335
MaeII ACGT 3 cut(s) 9, 1390, 2596
MaeIII GTNAC 9 cut(s) 247, 500, 682, 713, 1386, 1549, 1929, 2443, 2936
MalI GATC 6 cut(s) 38, 659, 795, 1440, 3286, 3460
MbiI CCGCTC 2 cut(s) 592, 3070
MboI GATC 6 cut(s) 36, 657, 793, 1438, 3284, 3458
MfeI CAATTG 3 cut(s) 1407, 2362, 2973
MflI RGATCY 1 cut(s) 3284
MhlI GDGCHC 3 cut(s) 1993, 2065, 2139
MlyI GAGTC 7 cut(s) 443, 556, 686, 890, 2108, 2410, 3052
MmeI TCCRAC 2 cut(s) 625, 753
Mph1103I ATGCAT 1 cut(s) 2569
MroNI GCCGGC 1 cut(s) 1771
MroXI GAANNNNTTC 2 cut(s) 2382, 2724
MslI CAYNNNNRTG 3 cut(s) 507, 609, 3080
MspA1I CMGCKG 1 cut(s) 2734
MspI CCGG 4 cut(s) 187, 1772, 1977, 3464
MspR9I CCNGG 5 cut(s) 381, 506, 1977, 2307, 2542
MunI CAATTG 3 cut(s) 1407, 2362, 2973
Mva1269I GAATGC 1 cut(s) 1220
MvaI CCWGG 4 cut(s) 381, 506, 2307, 2542
MvnI CGCG 1 cut(s) 2449
MwoI GCNNNNNNNGC 8 cut(s) 202, 224, 575, 745, 2060, 2237, 2448, 2707
NaeI GCCGGC 1 cut(s) 1773
NciI CCSGG 1 cut(s) 1977
NdeI CATATG 1 cut(s) 2486
NdeII GATC 6 cut(s) 36, 657, 793, 1438, 3284, 3458
NgoMIV GCCGGC 1 cut(s) 1771
NmuCI GTSAC 4 cut(s) 500, 713, 2443, 2936
NsbI TGCGCA 1 cut(s) 2738
NsiI ATGCAT 1 cut(s) 2569
NspI RCATGY 1 cut(s) 122
NspV TTCGAA 2 cut(s) 1851, 3139
PagI TCATGA 4 cut(s) 220, 660, 888, 1435
PciSI GCTCTTC 2 cut(s) 445, 3514
PctI GAATGC 1 cut(s) 1220
PdiI GCCGGC 1 cut(s) 1773
PdmI GAANNNNTTC 2 cut(s) 2382, 2724
PfeI GAWTC 8 cut(s) 1466, 1786, 1972, 2005, 2310, 2398, 2921, 3173
PflFI GACNNNGTC 1 cut(s) 308
PflMI CCANNNNNTGG 3 cut(s) 2393, 2865, 3098
PfoI TCCNGGA 1 cut(s) 2305
PkrI GCNGC 9 cut(s) 217, 747, 1057, 1198, 2655, 2736, 3210, 3233, 3506
PleI GAGTC 7 cut(s) 442, 556, 685, 890, 2107, 2410, 3051
PpsI GAGTC 7 cut(s) 442, 556, 685, 890, 2107, 2410, 3051
Ppu21I YACGTR 2 cut(s) 1391, 2597
PshAI GACNNNNGTC 1 cut(s) 629
PsiI TTATAA 1 cut(s) 1536
Psp124BI GAGCTC 1 cut(s) 2065
Psp6I CCWGG 4 cut(s) 379, 504, 2305, 2540
PspFI CCCAGC 1 cut(s) 945
PspGI CCWGG 4 cut(s) 379, 504, 2305, 2540
PstI CTGCAG 2 cut(s) 3020, 3213
PstNI CAGNNNCTG 2 cut(s) 1948, 3217
PsuI RGATCY 1 cut(s) 3284
PsyI GACNNNGTC 1 cut(s) 308
PvuII CAGCTG 1 cut(s) 2734
RsaI GTAC 6 cut(s) 385, 1047, 2192, 2479, 3086, 3496
RsaNI GTAC 6 cut(s) 384, 1046, 2191, 2478, 3085, 3495
RseI CAYNNNNRTG 3 cut(s) 507, 609, 3080
SacI GAGCTC 1 cut(s) 2065
SapI GCTCTTC 2 cut(s) 445, 3514
SatI GCNGC 9 cut(s) 216, 746, 1056, 1197, 2654, 2735, 3209, 3232, 3505
Sau3AI GATC 6 cut(s) 36, 657, 793, 1438, 3284, 3458
SchI GAGTC 7 cut(s) 443, 556, 686, 890, 2108, 2410, 3052
ScrFI CCNGG 5 cut(s) 381, 506, 1977, 2307, 2542
SduI GDGCHC 3 cut(s) 1993, 2065, 2139
SexAI ACCWGGT 1 cut(s) 2540
SfcI CTRYAG 2 cut(s) 3016, 3209
SfuI TTCGAA 2 cut(s) 1851, 3139
SmiMI CAYNNNNRTG 3 cut(s) 507, 609, 3080
SmlI CTYRAG 6 cut(s) 206, 2411, 2578, 3065, 3154, 3386
SmoI CTYRAG 6 cut(s) 206, 2411, 2578, 3065, 3154, 3386
SnaBI TACGTA 1 cut(s) 2597
SsiI CCGC 6 cut(s) 592, 868, 2212, 2240, 2656, 3070
SspI AATATT 3 cut(s) 2026, 2095, 2157
SspMI CTAG 7 cut(s) 417, 1301, 1994, 2288, 2769, 2927, 3335
SstI GAGCTC 1 cut(s) 2065
StyD4I CCNGG 5 cut(s) 379, 504, 1975, 2305, 2540
StyI CCWWGG 4 cut(s) 123, 2392, 3097, 3164
TaiI ACGT 3 cut(s) 12, 1393, 2599
TaqI TCGA 9 cut(s) 373, 730, 1207, 1740, 1851, 2546, 2957, 3041, 3139
TatI WGTACW 3 cut(s) 2477, 3084, 3494
TfiI GAWTC 8 cut(s) 1466, 1786, 1972, 2005, 2310, 2398, 2921, 3173
TscAI CASTG 8 cut(s) 1953, 2524, 2712, 2820, 2845, 2850, 3218, 3415
TseFI GTSAC 4 cut(s) 500, 713, 2443, 2936
TseI GCWGC 9 cut(s) 215, 745, 1055, 1196, 2653, 2734, 3208, 3231, 3504
Tsp45I GTSAC 4 cut(s) 500, 713, 2443, 2936
TspGWI ACGGA 1 cut(s) 1459
TspRI CASTG 8 cut(s) 1953, 2524, 2712, 2820, 2845, 2850, 3218, 3415
Tth111I GACNNNGTC 1 cut(s) 308
Van91I CCANNNNNTGG 3 cut(s) 2393, 2865, 3098
VneI GTGCAC 2 cut(s) 1989, 2135
XapI RAATTY 8 cut(s) 904, 1158, 1250, 1512, 1999, 2247, 2275, 3238
XbaI TCTAGA 2 cut(s) 1300, 3334
XceI RCATGY 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 3400
XmnI GAANNNNTTC 2 cut(s) 2382, 2724
XspI CTAG 7 cut(s) 417, 1301, 1994, 2288, 2769, 2927, 3335
Zsp2I ATGCAT 1 cut(s) 2569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.