MD10G1042700.v1.1

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000359
Physical Location & Seq
Reverse (-)
5540869 .. 5545481
4613 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1042700.v1.1.491

Sequence Viewer

Length: 645 bp
ATGGGCAACTGTAACAACATGGATTTCCTGGTCTTAGCTCTTGTAGGGTTTATTTTCCTGGGCAAGGCTAATGGCGCTATCCATTATTATGATTTCGTTCTGCAAGAAACAAACTTTACAAGGCTTTGTAGCACAAAGAGTATCTTAACTGTGAATGGAACTCTTCCGGGACCAACCATAACTGTTCACAGAGGAGACACCGCTTTTATCAATGTTCACAACCAAGGATATTACGGTGTCACCCTTCACTGGCATGGAGTGAAGCAACCAAGAAATCCATGGTCAGATGGACCTGAGAATATCACTCAATGTCCTATTCAACCAGGAACAAACTTCACGTACGAAGTTATATTCTCCAATGAAGAAGGAACATTATGGTGGCATGCTCATAGTGACTGGACGCGCGCCACCGTCCATGGTGCAATCATTATTCTACCGGCTGCCGGCACCACTTATCCATTTGCAACACCAGATGCACAAGAAACAGTTATTCTAGAGACAACATACCGATGGATAGTTGATTACGGTAAGACCTATCTTCTCAAGGTAATCAACGCCGTGATGAACGAAGAACAGTTCTTTGCCATCGCGAAAAACAGTCTACACAAGCGTACACAAAACAGACGTTCATACAAATACCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

215

Amino Acids

24.3

Weight (kDa)

7.12

Isoelectric Point (pI)

30.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 35 - 147 5.8e-40 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000359)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48100
fragaria_vesca FvH4_1g24380 FvH4_2g05800 FvH4_5g30730
malus_domestica MD01G1234900.v1.1 MD01G1235100.v1.1 MD01G1235200.v1.1 MD07G1307400.v1.1 MD07G1308000.v1.1 MD10G1042300.v1.1 MD10G1042400.v1.1 MD10G1042500.v1.1 MD10G1042700.v1.1
prunus_persica Prupe.2G325200_v2.0.a1 Prupe.6G242000_v2.0.a1 Prupe.8G046800_v2.0.a1 Prupe.8G046900_v2.0.a1 Prupe.8G046900_v2.0.a1 Prupe.8G046900_v2.0.a1
pyrus_communis pycom01g24320 pycom01g24330 pycom07g28020 pycom10g02950 pycom17g03690
rosa_chinensis RchiOBHm_Chr1g0384031 RchiOBHm_Chr2g0120941 RchiOBHm_Chr2g0120971 RchiOBHm_Chr2g0121051 RchiOBHm_Chr2g0121181 RchiOBHm_Chr2g0121211 RchiOBHm_Chr3g0473931 RchiOBHm_Chr3g0473941 RchiOBHm_Chr6g0257941 RchiOBHm_Chr6g0284781
rosa_laevigata RLG00000008541 RLG00000014737 RLG00000018604 RLG00000018607 RLG00000018608 RLG00000018610 RLG00000018611 RLG00000018613 RLG00000023967 RLG00000023970 RLG00000026078 RLG00000035183
rosa_multiflora Rmu_sc0000079.1_g000043 Rmu_sc0000079.1_g000062 Rmu_sc0000079.1_g000069 Rmu_sc0000621.1_g000019 Rmu_sc0000621.1_g000025 Rmu_sc0000621.1_g000041 Rmu_sc0000652.1_g000018 Rmu_sc0002414.1_g000030 Rmu_sc0002414.1_g000033 Rmu_sc0002843.1_g000016 Rmu_sc0005137.1_g000035 Rmu_sc0005139.1_g000021
rosa_roxburghii Rroxscaffold_2G00122530 Rroxscaffold_2G00122570 Rroxscaffold_2G00122580 Rroxscaffold_2G00122650 Rroxscaffold_4G00277080 Rroxscaffold_6G00407610 Rroxscaffold_6G00407620 Rroxscaffold_7G00209050
rosa_rugosa Rorug01G0444300 Rorug02G0232700 Rorug02G0232700 Rorug02G0232900 Rorug03G0137100 Rorug05G0565600 Rorug05G0566500
rosa_samantha Rh1BG427300 Rh1CG440100 Rh2CG278100 Rh2CG278200 Rh2CG278700 Rh2CG278800 Rh2CG279100 Rh2DG314200 Rh2DG314300 Rh2DG314600 Rh3AG187200 Rh3DG211700 Rh3DG211800 Rh5AG356600 Rh6BG078000 Rh6BG078700 Rh6CG073300 Rh6DG070300 Rh6DG190200 Rh6DG274700 Rh7BG365900
rosa_wichuraiana Rw0G018400 Rw1G040270 Rw2G023160 Rw2G023170 Rw2G023190 Rw2G023200 Rw2G023220 Rw2G023230 Rw3G017140 Rw3G017150 Rw4G012840 Rw6G007580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 446
AccI GTMKAC 1 cut(s) 601
AccII CGCG 3 cut(s) 403, 405, 590
AciI CCGC 1 cut(s) 201
AfaI GTAC 2 cut(s) 341, 613
AfiI CCNNNNNNNGG 3 cut(s) 64, 249, 443
AgsI TTSAA 1 cut(s) 320
AjnI CCWGG 3 cut(s) 27, 57, 322
AluBI AGCT 1 cut(s) 38
AluI AGCT 1 cut(s) 38
Alw26I GTCTC 2 cut(s) 189, 491
ApeKI GCWGC 1 cut(s) 440
AspLEI GCGC 3 cut(s) 77, 405, 407
AspS9I GGNCC 2 cut(s) 170, 290
AsuC2I CCSGG 1 cut(s) 168
AsuHPI GGTGA 1 cut(s) 232
AvaII GGWCC 2 cut(s) 170, 290
BanI GGYRCC 1 cut(s) 446
BbvI GCAGC 1 cut(s) 427
BccI CCATC 3 cut(s) 281, 504, 593
BceAI ACGGC 1 cut(s) 542
BciT130I CCWGG 3 cut(s) 29, 59, 324
BcnI CCSGG 1 cut(s) 168
BcoDI GTCTC 2 cut(s) 189, 491
BfaI CTAG 1 cut(s) 494
BfoI RGCGCY 1 cut(s) 78
BisI GCNGC 1 cut(s) 441
BlsI GCNGC 1 cut(s) 442
Bme1390I CCNGG 4 cut(s) 29, 59, 168, 324
Bme18I GGWCC 2 cut(s) 170, 290
BmgT120I GGNCC 2 cut(s) 170, 290
BmiI GGNNCC 2 cut(s) 171, 448
BmrFI CCNGG 4 cut(s) 29, 59, 168, 324
BmsI GCATC 1 cut(s) 463
BpuEI CTTGAG 1 cut(s) 527
BpuMI CCSGG 1 cut(s) 168
BsaAI YACGTR 1 cut(s) 339
BsaJI CCNNGG 4 cut(s) 58, 223, 278, 415
Bsc4I CCNNNNNNNGG 3 cut(s) 64, 249, 443
Bse118I RCCGGY 2 cut(s) 436, 443
Bse1I ACTGG 2 cut(s) 254, 401
BseBI CCWGG 3 cut(s) 29, 59, 324
BseDI CCNNGG 4 cut(s) 58, 223, 278, 415
BseLI CCNNNNNNNGG 3 cut(s) 64, 249, 443
BseMII CTCAG 1 cut(s) 285
BseNI ACTGG 2 cut(s) 254, 401
BsePI GCGCGC 1 cut(s) 403
BseRI GAGGAG 1 cut(s) 207
BseXI GCAGC 1 cut(s) 427
Bsh1236I CGCG 3 cut(s) 403, 405, 590
BshNI GGYRCC 1 cut(s) 446
BsiSI CCGG 3 cut(s) 167, 437, 444
BsiWI CGTACG 1 cut(s) 339
BslFI GGGAC 1 cut(s) 183
BslI CCNNNNNNNGG 3 cut(s) 64, 249, 443
BsmAI GTCTC 2 cut(s) 189, 491
BsmFI GGGAC 1 cut(s) 183
Bsp19I CCATGG 2 cut(s) 278, 415
Bsp68I TCGCGA 1 cut(s) 590
BspACI CCGC 1 cut(s) 201
BspCNI CTCAG 1 cut(s) 286
BspFNI CGCG 3 cut(s) 403, 405, 590
BspLI GGNNCC 2 cut(s) 171, 448
BspT107I GGYRCC 1 cut(s) 446
BsrFI RCCGGY 2 cut(s) 436, 443
BsrI ACTGG 2 cut(s) 254, 401
BssAI RCCGGY 2 cut(s) 436, 443
BssECI CCNNGG 4 cut(s) 58, 223, 278, 415
BssHII GCGCGC 1 cut(s) 403
BssT1I CCWWGG 3 cut(s) 223, 278, 415
Bst2UI CCWGG 3 cut(s) 29, 59, 324
Bst4CI ACNGT 9 cut(s) 11, 151, 184, 236, 412, 487, 527, 576, 599
Bst6I CTCTTC 1 cut(s) 168
BstBAI YACGTR 1 cut(s) 339
BstC8I GCNNGC 3 cut(s) 384, 405, 445
BstDEI CTNAG 2 cut(s) 34, 294
BstDSI CCRYGG 2 cut(s) 278, 415
BstENI CCTNNNNNAGG 1 cut(s) 62
BstFNI CGCG 3 cut(s) 403, 405, 590
BstH2I RGCGCY 1 cut(s) 78
BstHHI GCGC 3 cut(s) 77, 405, 407
BstMAI GTCTC 2 cut(s) 189, 491
BstMWI GCNNNNNNNGC 1 cut(s) 74
BstNI CCWGG 3 cut(s) 29, 59, 324
BstNSI RCATGY 1 cut(s) 386
BstSCI CCNGG 4 cut(s) 27, 57, 166, 322
BstUI CGCG 3 cut(s) 403, 405, 590
BstV1I GCAGC 1 cut(s) 427
BtgI CCRYGG 2 cut(s) 278, 415
BtgZI GCGATG 1 cut(s) 571
BtsIMutI CAGTG 1 cut(s) 247
BtuMI TCGCGA 1 cut(s) 590
Cac8I GCNNGC 3 cut(s) 384, 405, 445
CfoI GCGC 3 cut(s) 77, 405, 407
Cfr10I RCCGGY 2 cut(s) 436, 443
Cfr13I GGNCC 2 cut(s) 170, 290
CseI GACGC 1 cut(s) 409
Csp6I GTAC 2 cut(s) 340, 612
CviAII CATG 5 cut(s) 19, 254, 279, 383, 416
CviJI RGCY 4 cut(s) 38, 68, 124, 440
CviKI_1 RGCY 4 cut(s) 38, 68, 124, 440
CviQI GTAC 2 cut(s) 340, 612
DdeI CTNAG 2 cut(s) 34, 294
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
Eco130I CCWWGG 3 cut(s) 223, 278, 415
Eco47I GGWCC 2 cut(s) 170, 290
EcoNI CCTNNNNNAGG 1 cut(s) 62
EcoRII CCWGG 3 cut(s) 27, 57, 322
EcoT14I CCWWGG 3 cut(s) 223, 278, 415
ErhI CCWWGG 3 cut(s) 223, 278, 415
FaeI CATG 5 cut(s) 22, 257, 282, 386, 419
FalI AAGNNNNNCTT 2 cut(s) 128, 160
FaqI GGGAC 1 cut(s) 183
FatI CATG 5 cut(s) 18, 253, 278, 382, 415
FblI GTMKAC 1 cut(s) 601
Fnu4HI GCNGC 1 cut(s) 441
Fsp4HI GCNGC 1 cut(s) 441
FspBI CTAG 1 cut(s) 494
GlaI GCGC 3 cut(s) 76, 404, 406
GluI GCNGC 1 cut(s) 441
HaeII RGCGCY 1 cut(s) 78
HapII CCGG 3 cut(s) 167, 437, 444
HgaI GACGC 1 cut(s) 409
HhaI GCGC 3 cut(s) 77, 405, 407
Hin1II CATG 5 cut(s) 22, 257, 282, 386, 419
Hin6I GCGC 3 cut(s) 75, 403, 405
HinP1I GCGC 3 cut(s) 75, 403, 405
HpaII CCGG 3 cut(s) 167, 437, 444
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 4 cut(s) 187, 217, 602, 614
Hpy188I TCNGA 1 cut(s) 286
Hpy188III TCNNGA 2 cut(s) 494, 589
Hpy8I GTNNAC 4 cut(s) 187, 217, 602, 614
HpyAV CCTTC 2 cut(s) 254, 359
HpyCH4III ACNGT 9 cut(s) 11, 151, 184, 236, 412, 487, 527, 576, 599
HpyCH4IV ACGT 2 cut(s) 338, 625
HpyCH4V TGCA 4 cut(s) 103, 422, 464, 476
HpyF10VI GCNNNNNNNGC 1 cut(s) 74
HpyF3I CTNAG 2 cut(s) 34, 294
HpySE526I ACGT 2 cut(s) 338, 625
Hsp92II CATG 5 cut(s) 22, 257, 282, 386, 419
HspAI GCGC 3 cut(s) 75, 403, 405
KroI GCCGGC 1 cut(s) 443
KroNI GCCGGC 1 cut(s) 445
Lsp1109I GCAGC 1 cut(s) 427
LweI GCATC 1 cut(s) 463
MaeI CTAG 1 cut(s) 494
MaeII ACGT 2 cut(s) 338, 625
MaeIII GTNAC 3 cut(s) 11, 238, 392
MboII GAAGA 4 cut(s) 155, 374, 530, 581
MnlI CCTC 1 cut(s) 185
MroNI GCCGGC 1 cut(s) 443
MseI TTAA 1 cut(s) 146
MslI CAYNNNNRTG 4 cut(s) 87, 252, 376, 508
MspI CCGG 3 cut(s) 167, 437, 444
MspR9I CCNGG 4 cut(s) 29, 59, 168, 324
MvaI CCWGG 3 cut(s) 29, 59, 324
MvnI CGCG 3 cut(s) 403, 405, 590
MwoI GCNNNNNNNGC 1 cut(s) 74
NaeI GCCGGC 1 cut(s) 445
NciI CCSGG 1 cut(s) 168
NcoI CCATGG 2 cut(s) 278, 415
NgoMIV GCCGGC 1 cut(s) 443
NlaIII CATG 5 cut(s) 22, 257, 282, 386, 419
NlaIV GGNNCC 2 cut(s) 171, 448
NmuCI GTSAC 2 cut(s) 238, 392
NruI TCGCGA 1 cut(s) 590
NspI RCATGY 1 cut(s) 386
PaeI GCATGC 1 cut(s) 386
PauI GCGCGC 1 cut(s) 403
PdiI GCCGGC 1 cut(s) 445
Pfl23II CGTACG 1 cut(s) 339
PfoI TCCNGGA 1 cut(s) 166
PkrI GCNGC 1 cut(s) 442
Ppu21I YACGTR 1 cut(s) 339
Psp6I CCWGG 3 cut(s) 27, 57, 322
PspGI CCWGG 3 cut(s) 27, 57, 322
PspLI CGTACG 1 cut(s) 339
PspN4I GGNNCC 2 cut(s) 171, 448
PspPI GGNCC 2 cut(s) 170, 290
PteI GCGCGC 1 cut(s) 403
RruI TCGCGA 1 cut(s) 590
RsaI GTAC 2 cut(s) 341, 613
RsaNI GTAC 2 cut(s) 340, 612
RseI CAYNNNNRTG 4 cut(s) 87, 252, 376, 508
SaqAI TTAA 1 cut(s) 146
SatI GCNGC 1 cut(s) 441
Sau96I GGNCC 2 cut(s) 170, 290
ScrFI CCNGG 4 cut(s) 29, 59, 168, 324
SetI ASST 6 cut(s) 40, 295, 341, 536, 549, 628
SfaNI GCATC 1 cut(s) 463
SinI GGWCC 2 cut(s) 170, 290
SmiMI CAYNNNNRTG 4 cut(s) 87, 252, 376, 508
SmlI CTYRAG 1 cut(s) 542
SmoI CTYRAG 1 cut(s) 542
SphI GCATGC 1 cut(s) 386
SsiI CCGC 1 cut(s) 201
SspMI CTAG 1 cut(s) 494
StyD4I CCNGG 4 cut(s) 27, 57, 166, 322
StyI CCWWGG 3 cut(s) 223, 278, 415
TaaI ACNGT 9 cut(s) 11, 151, 184, 236, 412, 487, 527, 576, 599
TaiI ACGT 2 cut(s) 341, 628
Tru1I TTAA 1 cut(s) 146
Tru9I TTAA 1 cut(s) 146
TscAI CASTG 1 cut(s) 254
TseFI GTSAC 2 cut(s) 238, 392
TseI GCWGC 1 cut(s) 440
Tsp45I GTSAC 2 cut(s) 238, 392
TspDTI ATGAA 3 cut(s) 375, 578, 618
TspRI CASTG 1 cut(s) 254
VpaK11BI GGWCC 2 cut(s) 170, 290
XagI CCTNNNNNAGG 1 cut(s) 62
XbaI TCTAGA 1 cut(s) 493
XceI RCATGY 1 cut(s) 386
XcmI CCANNNNNNNNNTGG 1 cut(s) 276
XmiI GTMKAC 1 cut(s) 601
XspI CTAG 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.