Lignin degradation and detoxification of lignin-derived products
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 267 |
| AccB1I | GGYRCC | 1 cut(s) | 365 |
| AccII | CGCG | 1 cut(s) | 753 |
| AccIII | TCCGGA | 1 cut(s) | 470 |
| AciI | CCGC | 7 cut(s) | 495, 557, 669, 753, 915, 1019, 1055 |
| AclWI | GGATC | 2 cut(s) | 354, 1253 |
| AcoI | YGGCCR | 2 cut(s) | 80, 666 |
| AcsI | RAATTY | 1 cut(s) | 1012 |
| AcuI | CTGAAG | 1 cut(s) | 1049 |
| AdeI | CACNNNGTG | 1 cut(s) | 312 |
| AfaI | GTAC | 5 cut(s) | 185, 360, 773, 1061, 1106 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 388, 891, 1079, 1295 |
| AflIII | ACRYGT | 2 cut(s) | 768, 905 |
| AgsI | TTSAA | 5 cut(s) | 467, 786, 1133, 1243, 1318 |
| AjiI | CACGTC | 1 cut(s) | 1387 |
| AjnI | CCWGG | 2 cut(s) | 607, 1330 |
| AleI | CACNNNNGTG | 2 cut(s) | 98, 1115 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 991, 1023 |
| AluBI | AGCT | 8 cut(s) | 77, 86, 448, 567, 936, 968, 1123, 1210 |
| AluI | AGCT | 8 cut(s) | 77, 86, 448, 567, 936, 968, 1123, 1210 |
| Alw21I | GWGCWC | 1 cut(s) | 317 |
| Alw26I | GTCTC | 1 cut(s) | 192 |
| Alw44I | GTGCAC | 1 cut(s) | 313 |
| AlwI | GGATC | 2 cut(s) | 354, 1253 |
| Aor13HI | TCCGGA | 1 cut(s) | 470 |
| AoxI | GGCC | 5 cut(s) | 80, 666, 893, 1320, 1414 |
| ApaI | GGGCCC | 1 cut(s) | 1418 |
| ApaLI | GTGCAC | 1 cut(s) | 313 |
| ApoI | RAATTY | 1 cut(s) | 1012 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 740, 772 |
| AseI | ATTAAT | 1 cut(s) | 51 |
| AspLEI | GCGC | 2 cut(s) | 268, 890 |
| AspS9I | GGNCC | 8 cut(s) | 173, 293, 478, 893, 943, 1234, 1414, 1415 |
| AsuC2I | CCSGG | 3 cut(s) | 171, 519, 892 |
| AsuHPI | GGTGA | 4 cut(s) | 350, 597, 1054, 1102 |
| AsuNHI | GCTAGC | 2 cut(s) | 563, 917 |
| AvaII | GGWCC | 5 cut(s) | 173, 293, 478, 943, 1234 |
| BaeGI | GKGCMC | 2 cut(s) | 317, 1418 |
| BalI | TGGCCA | 1 cut(s) | 82 |
| BanI | GGYRCC | 1 cut(s) | 365 |
| BanII | GRGCYC | 1 cut(s) | 1418 |
| Bbv12I | GWGCWC | 1 cut(s) | 317 |
| BccI | CCATC | 3 cut(s) | 377, 1170, 1362 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 35 |
| BciT130I | CCWGG | 2 cut(s) | 609, 1332 |
| BcnI | CCSGG | 3 cut(s) | 171, 519, 892 |
| BcoDI | GTCTC | 1 cut(s) | 192 |
| BfaI | CTAG | 3 cut(s) | 564, 918, 948 |
| BfmI | CTRYAG | 1 cut(s) | 672 |
| BisI | GCNGC | 3 cut(s) | 669, 915, 1055 |
| BlsI | GCNGC | 3 cut(s) | 670, 916, 1056 |
| Bme1390I | CCNGG | 5 cut(s) | 171, 519, 609, 892, 1332 |
| Bme18I | GGWCC | 5 cut(s) | 173, 293, 478, 943, 1234 |
| BmgBI | CACGTC | 1 cut(s) | 1387 |
| BmgT120I | GGNCC | 8 cut(s) | 173, 293, 478, 893, 943, 1234, 1414, 1415 |
| BmiI | GGNNCC | 6 cut(s) | 367, 479, 480, 945, 1236, 1416 |
| BmrFI | CCNGG | 5 cut(s) | 171, 519, 609, 892, 1332 |
| BmsI | GCATC | 5 cut(s) | 66, 550, 839, 858, 1180 |
| BmtI | GCTAGC | 2 cut(s) | 567, 921 |
| BpmI | CTGGAG | 1 cut(s) | 1353 |
| BpuEI | CTTGAG | 1 cut(s) | 1404 |
| BpuMI | CCSGG | 3 cut(s) | 171, 519, 892 |
| BsaAI | YACGTR | 1 cut(s) | 771 |
| BsaBI | GATNNNNATC | 1 cut(s) | 528 |
| BsaJI | CCNNGG | 4 cut(s) | 272, 281, 1113, 1330 |
| BsaWI | WCCGGW | 2 cut(s) | 470, 1444 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 189, 219, 1112, 1142 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 388, 891, 1079, 1295 |
| Bse1I | ACTGG | 1 cut(s) | 257 |
| Bse3DI | GCAATG | 2 cut(s) | 67, 1348 |
| Bse8I | GATNNNNATC | 1 cut(s) | 528 |
| BseAI | TCCGGA | 1 cut(s) | 470 |
| BseBI | CCWGG | 2 cut(s) | 609, 1332 |
| BseDI | CCNNGG | 4 cut(s) | 272, 281, 1113, 1330 |
| BseGI | GGATG | 3 cut(s) | 57, 565, 1162 |
| BseJI | GATNNNNATC | 1 cut(s) | 528 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 388, 891, 1079, 1295 |
| BseMI | GCAATG | 2 cut(s) | 67, 1348 |
| BseNI | ACTGG | 1 cut(s) | 257 |
| BseRI | GAGGAG | 2 cut(s) | 53, 1254 |
| BseSI | GKGCMC | 2 cut(s) | 317, 1418 |
| BseYI | CCCAGC | 1 cut(s) | 692 |
| Bsh1236I | CGCG | 1 cut(s) | 753 |
| BshFI | GGCC | 5 cut(s) | 82, 668, 895, 1322, 1416 |
| BshNI | GGYRCC | 1 cut(s) | 365 |
| BsiHKAI | GWGCWC | 1 cut(s) | 317 |
| BsiSI | CCGG | 6 cut(s) | 171, 471, 519, 846, 891, 1445 |
| BslFI | GGGAC | 6 cut(s) | 242, 491, 916, 1096, 1150, 1271 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 388, 891, 1079, 1295 |
| BsmAI | GTCTC | 1 cut(s) | 192 |
| BsmFI | GGGAC | 6 cut(s) | 242, 491, 916, 1096, 1150, 1271 |
| BsnI | GGCC | 5 cut(s) | 82, 668, 895, 1322, 1416 |
| Bsp120I | GGGCCC | 1 cut(s) | 1414 |
| Bsp1286I | GDGCHC | 2 cut(s) | 317, 1418 |
| Bsp13I | TCCGGA | 1 cut(s) | 470 |
| Bsp1407I | TGTACA | 1 cut(s) | 358 |
| Bsp143I | GATC | 5 cut(s) | 346, 432, 523, 600, 1258 |
| Bsp19I | CCATGG | 1 cut(s) | 281 |
| BspACI | CCGC | 7 cut(s) | 495, 557, 669, 753, 915, 1019, 1055 |
| BspANI | GGCC | 5 cut(s) | 82, 668, 895, 1322, 1416 |
| BspEI | TCCGGA | 1 cut(s) | 470 |
| BspFNI | CGCG | 1 cut(s) | 753 |
| BspHI | TCATGA | 1 cut(s) | 418 |
| BspLI | GGNNCC | 6 cut(s) | 367, 479, 480, 945, 1236, 1416 |
| BspOI | GCTAGC | 2 cut(s) | 567, 921 |
| BspPI | GGATC | 2 cut(s) | 354, 1253 |
| BspT107I | GGYRCC | 1 cut(s) | 365 |
| BsrDI | GCAATG | 2 cut(s) | 67, 1348 |
| BsrGI | TGTACA | 1 cut(s) | 358 |
| BsrI | ACTGG | 1 cut(s) | 257 |
| BssECI | CCNNGG | 4 cut(s) | 272, 281, 1113, 1330 |
| BssMI | GATC | 5 cut(s) | 346, 432, 523, 600, 1258 |
| BssT1I | CCWWGG | 2 cut(s) | 272, 281 |
| Bst2UI | CCWGG | 2 cut(s) | 609, 1332 |
| Bst4CI | ACNGT | 9 cut(s) | 154, 205, 338, 358, 512, 1042, 1117, 1187, 1279 |
| Bst6I | CTCTTC | 2 cut(s) | 810, 1272 |
| BstAUI | TGTACA | 1 cut(s) | 358 |
| BstBAI | YACGTR | 1 cut(s) | 771 |
| BstC8I | GCNNGC | 6 cut(s) | 20, 84, 190, 330, 565, 919 |
| BstDSI | CCRYGG | 2 cut(s) | 281, 1113 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1293 |
| BstF5I | GGATG | 3 cut(s) | 57, 565, 1162 |
| BstFNI | CGCG | 1 cut(s) | 753 |
| BstHHI | GCGC | 2 cut(s) | 268, 890 |
| BstKTI | GATC | 5 cut(s) | 349, 435, 526, 603, 1261 |
| BstMAI | GTCTC | 1 cut(s) | 192 |
| BstMBI | GATC | 5 cut(s) | 346, 432, 523, 600, 1258 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 83, 674 |
| BstNI | CCWGG | 2 cut(s) | 609, 1332 |
| BstNSI | RCATGY | 1 cut(s) | 332 |
| BstSCI | CCNGG | 5 cut(s) | 169, 517, 607, 890, 1330 |
| BstSFI | CTRYAG | 1 cut(s) | 672 |
| BstSLI | GKGCMC | 2 cut(s) | 317, 1418 |
| BstUI | CGCG | 1 cut(s) | 753 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 257 |
| BsuRI | GGCC | 5 cut(s) | 82, 668, 895, 1322, 1416 |
| BtgI | CCRYGG | 2 cut(s) | 281, 1113 |
| BtrI | CACGTC | 1 cut(s) | 1387 |
| BtsCI | GGATG | 3 cut(s) | 57, 565, 1162 |
| BtsI | GCAGTG | 2 cut(s) | 317, 633 |
| BtsIMutI | CAGTG | 4 cut(s) | 250, 317, 354, 633 |
| Cac8I | GCNNGC | 6 cut(s) | 20, 84, 190, 330, 565, 919 |
| CciI | TCATGA | 1 cut(s) | 418 |
| CfoI | GCGC | 2 cut(s) | 268, 890 |
| Cfr13I | GGNCC | 8 cut(s) | 173, 293, 478, 893, 943, 1234, 1414, 1415 |
| CpoI | CGGWCCG | 1 cut(s) | 173 |
| CsiI | ACCWGGT | 1 cut(s) | 607 |
| Csp6I | GTAC | 5 cut(s) | 184, 359, 772, 1060, 1105 |
| CspI | CGGWCCG | 1 cut(s) | 173 |
| CviQI | GTAC | 5 cut(s) | 184, 359, 772, 1060, 1105 |
| DpnI | GATC | 5 cut(s) | 348, 434, 525, 602, 1260 |
| DpnII | GATC | 5 cut(s) | 346, 432, 523, 600, 1258 |
| DraIII | CACNNNGTG | 1 cut(s) | 312 |
| EaeI | YGGCCR | 2 cut(s) | 80, 666 |
| Eam1104I | CTCTTC | 2 cut(s) | 810, 1272 |
| EarI | CTCTTC | 2 cut(s) | 810, 1272 |
| EciI | GGCGGA | 1 cut(s) | 572 |
| Eco130I | CCWWGG | 2 cut(s) | 272, 281 |
| Eco24I | GRGCYC | 1 cut(s) | 1418 |
| Eco47I | GGWCC | 5 cut(s) | 173, 293, 478, 943, 1234 |
| Eco57I | CTGAAG | 1 cut(s) | 1049 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1293 |
| EcoO109I | RGGNCCY | 4 cut(s) | 478, 1234, 1414, 1415 |
| EcoRII | CCWGG | 2 cut(s) | 607, 1330 |
| EcoT14I | CCWWGG | 2 cut(s) | 272, 281 |
| EcoT22I | ATGCAT | 3 cut(s) | 832, 1173, 1350 |
| EcoT38I | GRGCYC | 1 cut(s) | 1418 |
| ErhI | CCWWGG | 2 cut(s) | 272, 281 |
| FaqI | GGGAC | 6 cut(s) | 242, 491, 916, 1096, 1150, 1271 |
| FauI | CCCGC | 2 cut(s) | 746, 1026 |
| FauNDI | CATATG | 1 cut(s) | 1368 |
| Fnu4HI | GCNGC | 3 cut(s) | 669, 915, 1055 |
| FokI | GGATG | 3 cut(s) | 44, 572, 1149 |
| FriOI | GRGCYC | 1 cut(s) | 1418 |
| Fsp4HI | GCNGC | 3 cut(s) | 669, 915, 1055 |
| FspBI | CTAG | 3 cut(s) | 564, 918, 948 |
| FspI | TGCGCA | 1 cut(s) | 267 |
| GlaI | GCGC | 2 cut(s) | 267, 889 |
| GluI | GCNGC | 3 cut(s) | 669, 915, 1055 |
| GsaI | CCCAGC | 1 cut(s) | 696 |
| GsuI | CTGGAG | 1 cut(s) | 1353 |
| HaeIII | GGCC | 5 cut(s) | 82, 668, 895, 1322, 1416 |
| HapII | CCGG | 6 cut(s) | 171, 471, 519, 846, 891, 1445 |
| HhaI | GCGC | 2 cut(s) | 268, 890 |
| Hin6I | GCGC | 2 cut(s) | 266, 888 |
| HinP1I | GCGC | 2 cut(s) | 266, 888 |
| HincII | GTYRAC | 3 cut(s) | 515, 859, 985 |
| HindII | GTYRAC | 3 cut(s) | 515, 859, 985 |
| HindIII | AAGCTT | 3 cut(s) | 75, 446, 966 |
| HinfI | GANTC | 4 cut(s) | 277, 542, 1069, 1314 |
| HpaII | CCGG | 6 cut(s) | 171, 471, 519, 846, 891, 1445 |
| HphI | GGTGA | 4 cut(s) | 350, 597, 1054, 1102 |
| Hpy166II | GTNNAC | 7 cut(s) | 315, 515, 774, 859, 925, 943, 985 |
| Hpy188I | TCNGA | 6 cut(s) | 289, 541, 645, 1068, 1074, 1272 |
| Hpy188III | TCNNGA | 4 cut(s) | 419, 430, 471, 1358 |
| Hpy8I | GTNNAC | 7 cut(s) | 315, 515, 774, 859, 925, 943, 985 |
| Hpy99I | CGWCG | 1 cut(s) | 215 |
| HpyAV | CCTTC | 5 cut(s) | 789, 801, 1039, 1073, 1087 |
| HpyCH4III | ACNGT | 9 cut(s) | 154, 205, 338, 358, 512, 1042, 1117, 1187, 1279 |
| HpyCH4IV | ACGT | 3 cut(s) | 770, 907, 1386 |
| HpyCH4V | TGCA | 8 cut(s) | 22, 315, 739, 830, 1171, 1177, 1292, 1348 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 83, 674 |
| HpySE526I | ACGT | 3 cut(s) | 770, 907, 1386 |
| HspAI | GCGC | 2 cut(s) | 266, 888 |
| KflI | GGGWCCC | 1 cut(s) | 478 |
| Kpn2I | TCCGGA | 1 cut(s) | 470 |
| Kzo9I | GATC | 5 cut(s) | 346, 432, 523, 600, 1258 |
| LmnI | GCTCC | 2 cut(s) | 1120, 1207 |
| LweI | GCATC | 5 cut(s) | 66, 550, 839, 858, 1180 |
| MabI | ACCWGGT | 1 cut(s) | 607 |
| MaeI | CTAG | 3 cut(s) | 564, 918, 948 |
| MaeII | ACGT | 3 cut(s) | 770, 907, 1386 |
| MaeIII | GTNAC | 6 cut(s) | 89, 236, 703, 1181, 1387, 1431 |
| MalI | GATC | 5 cut(s) | 348, 434, 525, 602, 1260 |
| MboI | GATC | 5 cut(s) | 346, 432, 523, 600, 1258 |
| MboII | GAAGA | 6 cut(s) | 16, 119, 434, 774, 797, 1259 |
| MhlI | GDGCHC | 2 cut(s) | 317, 1418 |
| MlsI | TGGCCA | 1 cut(s) | 82 |
| MluCI | AATT | 9 cut(s) | 397, 487, 763, 838, 880, 951, 1012, 1033, 1141 |
| MluNI | TGGCCA | 1 cut(s) | 82 |
| MmeI | TCCRAC | 2 cut(s) | 267, 1180 |
| Mox20I | TGGCCA | 1 cut(s) | 82 |
| Mph1103I | ATGCAT | 3 cut(s) | 832, 1173, 1350 |
| MroI | TCCGGA | 1 cut(s) | 470 |
| MscI | TGGCCA | 1 cut(s) | 82 |
| MseI | TTAA | 6 cut(s) | 51, 222, 507, 837, 1032, 1462 |
| MslI | CAYNNNNRTG | 5 cut(s) | 98, 255, 1115, 1176, 1385 |
| Msp20I | TGGCCA | 1 cut(s) | 82 |
| MspA1I | CMGCKG | 1 cut(s) | 1057 |
| MspI | CCGG | 6 cut(s) | 171, 471, 519, 846, 891, 1445 |
| MspR9I | CCNGG | 5 cut(s) | 171, 519, 609, 892, 1332 |
| MvaI | CCWGG | 2 cut(s) | 609, 1332 |
| MvnI | CGCG | 1 cut(s) | 753 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 83, 674 |
| NciI | CCSGG | 3 cut(s) | 171, 519, 892 |
| NcoI | CCATGG | 1 cut(s) | 281 |
| NdeI | CATATG | 1 cut(s) | 1368 |
| NdeII | GATC | 5 cut(s) | 346, 432, 523, 600, 1258 |
| NheI | GCTAGC | 2 cut(s) | 563, 917 |
| NlaIV | GGNNCC | 6 cut(s) | 367, 479, 480, 945, 1236, 1416 |
| NmuCI | GTSAC | 1 cut(s) | 1387 |
| NsbI | TGCGCA | 1 cut(s) | 267 |
| NsiI | ATGCAT | 3 cut(s) | 832, 1173, 1350 |
| NspI | RCATGY | 1 cut(s) | 332 |
| OliI | CACNNNNGTG | 2 cut(s) | 98, 1115 |
| PaeI | GCATGC | 1 cut(s) | 332 |
| PagI | TCATGA | 1 cut(s) | 418 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 918 |
| PfeI | GAWTC | 4 cut(s) | 277, 542, 1069, 1314 |
| PflFI | GACNNNGTC | 1 cut(s) | 203 |
| PfoI | TCCNGGA | 1 cut(s) | 169 |
| PkrI | GCNGC | 3 cut(s) | 670, 916, 1056 |
| Ppu21I | YACGTR | 1 cut(s) | 771 |
| PpuMI | RGGWCCY | 2 cut(s) | 478, 1234 |
| PshBI | ATTAAT | 1 cut(s) | 51 |
| Psp5II | RGGWCCY | 2 cut(s) | 478, 1234 |
| Psp6I | CCWGG | 2 cut(s) | 607, 1330 |
| PspFI | CCCAGC | 1 cut(s) | 692 |
| PspGI | CCWGG | 2 cut(s) | 607, 1330 |
| PspN4I | GGNNCC | 6 cut(s) | 367, 479, 480, 945, 1236, 1416 |
| PspOMI | GGGCCC | 1 cut(s) | 1414 |
| PspPI | GGNCC | 8 cut(s) | 173, 293, 478, 893, 943, 1234, 1414, 1415 |
| PspPPI | RGGWCCY | 2 cut(s) | 478, 1234 |
| PsyI | GACNNNGTC | 1 cut(s) | 203 |
| RsaI | GTAC | 5 cut(s) | 185, 360, 773, 1061, 1106 |
| RsaNI | GTAC | 5 cut(s) | 184, 359, 772, 1060, 1105 |
| RseI | CAYNNNNRTG | 5 cut(s) | 98, 255, 1115, 1176, 1385 |
| Rsr2I | CGGWCCG | 1 cut(s) | 173 |
| RsrII | CGGWCCG | 1 cut(s) | 173 |
| SaqAI | TTAA | 6 cut(s) | 51, 222, 507, 837, 1032, 1462 |
| SatI | GCNGC | 3 cut(s) | 669, 915, 1055 |
| Sau3AI | GATC | 5 cut(s) | 346, 432, 523, 600, 1258 |
| Sau96I | GGNCC | 8 cut(s) | 173, 293, 478, 893, 943, 1234, 1414, 1415 |
| ScrFI | CCNGG | 5 cut(s) | 171, 519, 609, 892, 1332 |
| SduI | GDGCHC | 2 cut(s) | 317, 1418 |
| SexAI | ACCWGGT | 1 cut(s) | 607 |
| SfaNI | GCATC | 5 cut(s) | 66, 550, 839, 858, 1180 |
| SfcI | CTRYAG | 1 cut(s) | 672 |
| SinI | GGWCC | 5 cut(s) | 173, 293, 478, 943, 1234 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 98, 255, 1115, 1176, 1385 |
| SmlI | CTYRAG | 1 cut(s) | 1419 |
| SmoI | CTYRAG | 1 cut(s) | 1419 |
| SphI | GCATGC | 1 cut(s) | 332 |
| Sse9I | AATT | 9 cut(s) | 397, 487, 763, 838, 880, 951, 1012, 1033, 1141 |
| SsiI | CCGC | 7 cut(s) | 495, 557, 669, 753, 915, 1019, 1055 |
| SspMI | CTAG | 3 cut(s) | 564, 918, 948 |
| StyD4I | CCNGG | 5 cut(s) | 169, 517, 607, 890, 1330 |
| StyI | CCWWGG | 2 cut(s) | 272, 281 |
| TaaI | ACNGT | 9 cut(s) | 154, 205, 338, 358, 512, 1042, 1117, 1187, 1279 |
| TaiI | ACGT | 3 cut(s) | 773, 910, 1389 |
| TaqI | TCGA | 5 cut(s) | 11, 213, 349, 599, 1257 |
| TaqII | GACCGA | 1 cut(s) | 190 |
| TasI | AATT | 9 cut(s) | 397, 487, 763, 838, 880, 951, 1012, 1033, 1141 |
| TatI | WGTACW | 2 cut(s) | 358, 1104 |
| TauI | GCSGC | 3 cut(s) | 671, 917, 1057 |
| TfiI | GAWTC | 4 cut(s) | 277, 542, 1069, 1314 |
| Tru1I | TTAA | 6 cut(s) | 51, 222, 507, 837, 1032, 1462 |
| Tru9I | TTAA | 6 cut(s) | 51, 222, 507, 837, 1032, 1462 |
| TscAI | CASTG | 4 cut(s) | 257, 317, 361, 640 |
| TseFI | GTSAC | 1 cut(s) | 1387 |
| Tsp45I | GTSAC | 1 cut(s) | 1387 |
| TspDTI | ATGAA | 3 cut(s) | 17, 435, 1384 |
| TspGWI | ACGGA | 3 cut(s) | 306, 844, 1100 |
| TspRI | CASTG | 4 cut(s) | 257, 317, 361, 640 |
| Tth111I | GACNNNGTC | 1 cut(s) | 203 |
| VneI | GTGCAC | 1 cut(s) | 313 |
| VpaK11BI | GGWCC | 5 cut(s) | 173, 293, 478, 943, 1234 |
| VspI | ATTAAT | 1 cut(s) | 51 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1293 |
| XapI | RAATTY | 1 cut(s) | 1012 |
| XceI | RCATGY | 1 cut(s) | 332 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 279 |
| XspI | CTAG | 3 cut(s) | 564, 918, 948 |
| Zsp2I | ATGCAT | 3 cut(s) | 832, 1173, 1350 |