Lignin degradation and detoxification of lignin-derived products
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 87, 186 |
| AccII | CGCG | 1 cut(s) | 758 |
| AciI | CCGC | 3 cut(s) | 756, 1533, 1682 |
| AclI | AACGTT | 1 cut(s) | 1655 |
| AclWI | GGATC | 4 cut(s) | 1166, 1179, 1345, 1481 |
| AcoI | YGGCCR | 1 cut(s) | 1408 |
| AcsI | RAATTY | 3 cut(s) | 158, 533, 1369 |
| AcuI | CTGAAG | 4 cut(s) | 14, 60, 495, 1293 |
| AdeI | CACNNNGTG | 1 cut(s) | 308 |
| AfaI | GTAC | 2 cut(s) | 54, 525 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 375, 406, 440, 730, 1100, 1523, 1603 |
| AflII | CTTAAG | 1 cut(s) | 1519 |
| AhlI | ACTAGT | 1 cut(s) | 49 |
| AjnI | CCWGG | 6 cut(s) | 163, 439, 592, 790, 832, 1558 |
| AleI | CACNNNNGTG | 1 cut(s) | 1316 |
| AluBI | AGCT | 8 cut(s) | 74, 182, 450, 1142, 1164, 1196, 1416, 1473 |
| AluI | AGCT | 8 cut(s) | 74, 182, 450, 1142, 1164, 1196, 1416, 1473 |
| Alw26I | GTCTC | 1 cut(s) | 591 |
| AlwI | GGATC | 4 cut(s) | 1166, 1179, 1345, 1481 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1511 |
| Ama87I | CYCGRG | 2 cut(s) | 399, 461 |
| AoxI | GGCC | 3 cut(s) | 1408, 1530, 1548 |
| ApeKI | GCWGC | 8 cut(s) | 126, 680, 737, 746, 851, 992, 1142, 1660 |
| ApoI | RAATTY | 3 cut(s) | 158, 533, 1369 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1066 |
| AspLEI | GCGC | 1 cut(s) | 760 |
| AspS9I | GGNCC | 8 cut(s) | 167, 279, 287, 396, 643, 1121, 1462, 1530 |
| AsuC2I | CCSGG | 3 cut(s) | 321, 400, 401 |
| AsuHPI | GGTGA | 4 cut(s) | 506, 1093, 1330, 1586 |
| AvaI | CYCGRG | 2 cut(s) | 399, 461 |
| AvaII | GGWCC | 7 cut(s) | 167, 279, 287, 396, 643, 1121, 1462 |
| BaeGI | GKGCMC | 1 cut(s) | 1518 |
| BalI | TGGCCA | 1 cut(s) | 1410 |
| BamHI | GGATCC | 1 cut(s) | 1171 |
| BanII | GRGCYC | 1 cut(s) | 468 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 45, 77 |
| BbsI | GAAGAC | 2 cut(s) | 1281, 1612 |
| BbvI | GCAGC | 8 cut(s) | 138, 667, 749, 758, 838, 1004, 1129, 1647 |
| BccI | CCATC | 4 cut(s) | 278, 587, 920, 1521 |
| BceAI | ACGGC | 2 cut(s) | 1284, 1669 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 452, 486 |
| BciT130I | CCWGG | 6 cut(s) | 165, 441, 594, 792, 834, 1560 |
| BcnI | CCSGG | 3 cut(s) | 321, 400, 401 |
| BcoDI | GTCTC | 1 cut(s) | 591 |
| BcuI | ACTAGT | 1 cut(s) | 49 |
| BfaI | CTAG | 5 cut(s) | 50, 447, 855, 875, 1025 |
| BfrI | CTTAAG | 1 cut(s) | 1519 |
| BmcAI | AGTACT | 1 cut(s) | 54 |
| Bme1390I | CCNGG | 9 cut(s) | 165, 321, 400, 401, 441, 594, 792, 834, 1560 |
| Bme18I | GGWCC | 7 cut(s) | 167, 279, 287, 396, 643, 1121, 1462 |
| BmeT110I | CYCGRG | 2 cut(s) | 399, 461 |
| BmgT120I | GGNCC | 8 cut(s) | 167, 279, 287, 396, 643, 1121, 1462, 1530 |
| BmiI | GGNNCC | 5 cut(s) | 367, 398, 1123, 1173, 1464 |
| BmrFI | CCNGG | 9 cut(s) | 165, 321, 400, 401, 441, 594, 792, 834, 1560 |
| BmsI | GCATC | 4 cut(s) | 559, 702, 796, 1408 |
| BoxI | GACNNNNGTC | 1 cut(s) | 731 |
| BpiI | GAAGAC | 2 cut(s) | 1281, 1612 |
| BpmI | CTGGAG | 1 cut(s) | 1581 |
| BpuMI | CCSGG | 3 cut(s) | 321, 400, 401 |
| BsaI | GGTCTC | 1 cut(s) | 591 |
| BsaJI | CCNNGG | 5 cut(s) | 266, 275, 399, 865, 1099 |
| BsaWI | WCCGGW | 1 cut(s) | 545 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 183, 213, 772, 802 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 375, 406, 440, 730, 1100, 1523, 1603 |
| Bse1I | ACTGG | 4 cut(s) | 170, 251, 1146, 1438 |
| Bse3DI | GCAATG | 2 cut(s) | 937, 1029 |
| BseBI | CCWGG | 6 cut(s) | 165, 441, 594, 792, 834, 1560 |
| BseDI | CCNNGG | 5 cut(s) | 266, 275, 399, 865, 1099 |
| BseGI | GGATG | 4 cut(s) | 693, 1348, 1390, 1532 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 375, 406, 440, 730, 1100, 1523, 1603 |
| BseMI | GCAATG | 2 cut(s) | 937, 1029 |
| BseMII | CTCAG | 1 cut(s) | 720 |
| BseNI | ACTGG | 4 cut(s) | 170, 251, 1146, 1438 |
| BseRI | GAGGAG | 2 cut(s) | 1482, 1656 |
| BseSI | GKGCMC | 1 cut(s) | 1518 |
| BseXI | GCAGC | 8 cut(s) | 138, 667, 749, 758, 838, 1004, 1129, 1647 |
| BseYI | CCCAGC | 1 cut(s) | 1379 |
| BsgI | GTGCAG | 1 cut(s) | 145 |
| Bsh1236I | CGCG | 1 cut(s) | 758 |
| BshFI | GGCC | 3 cut(s) | 1410, 1532, 1550 |
| BsiHKCI | CYCGRG | 2 cut(s) | 399, 461 |
| BsiSI | CCGG | 4 cut(s) | 320, 400, 546, 1551 |
| BslFI | GGGAC | 4 cut(s) | 382, 955, 1144, 1322 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 375, 406, 440, 730, 1100, 1523, 1603 |
| BsmAI | GTCTC | 1 cut(s) | 591 |
| BsmFI | GGGAC | 4 cut(s) | 382, 955, 1144, 1322 |
| BsmI | GAATGC | 2 cut(s) | 994, 1614 |
| BsnI | GGCC | 3 cut(s) | 1410, 1532, 1550 |
| Bso31I | GGTCTC | 1 cut(s) | 591 |
| BsoBI | CYCGRG | 2 cut(s) | 399, 461 |
| Bsp1286I | GDGCHC | 2 cut(s) | 468, 1518 |
| Bsp143I | GATC | 5 cut(s) | 342, 1171, 1337, 1387, 1486 |
| Bsp19I | CCATGG | 1 cut(s) | 275 |
| BspACI | CCGC | 3 cut(s) | 756, 1533, 1682 |
| BspANI | GGCC | 3 cut(s) | 1410, 1532, 1550 |
| BspCNI | CTCAG | 1 cut(s) | 719 |
| BspFNI | CGCG | 1 cut(s) | 758 |
| BspLI | GGNNCC | 5 cut(s) | 367, 398, 1123, 1173, 1464 |
| BspPI | GGATC | 4 cut(s) | 1166, 1179, 1345, 1481 |
| BspTI | CTTAAG | 1 cut(s) | 1519 |
| BspTNI | GGTCTC | 1 cut(s) | 591 |
| BsrDI | GCAATG | 2 cut(s) | 937, 1029 |
| BsrI | ACTGG | 4 cut(s) | 170, 251, 1146, 1438 |
| BssECI | CCNNGG | 5 cut(s) | 266, 275, 399, 865, 1099 |
| BssMI | GATC | 5 cut(s) | 342, 1171, 1337, 1387, 1486 |
| BssT1I | CCWWGG | 3 cut(s) | 275, 865, 1099 |
| Bst2UI | CCWGG | 6 cut(s) | 165, 441, 594, 792, 834, 1560 |
| Bst6I | CTCTTC | 1 cut(s) | 62 |
| BstAFI | CTTAAG | 1 cut(s) | 1519 |
| BstC8I | GCNNGC | 1 cut(s) | 381 |
| BstDEI | CTNAG | 3 cut(s) | 132, 706, 1496 |
| BstDSI | CCRYGG | 1 cut(s) | 275 |
| BstEII | GGTNACC | 1 cut(s) | 869 |
| BstF5I | GGATG | 4 cut(s) | 693, 1348, 1390, 1532 |
| BstFNI | CGCG | 1 cut(s) | 758 |
| BstHHI | GCGC | 1 cut(s) | 760 |
| BstKTI | GATC | 5 cut(s) | 345, 1174, 1340, 1390, 1489 |
| BstMAI | GTCTC | 1 cut(s) | 591 |
| BstMBI | GATC | 5 cut(s) | 342, 1171, 1337, 1387, 1486 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 32, 743, 746, 755, 857, 1020 |
| BstNI | CCWGG | 6 cut(s) | 165, 441, 594, 792, 834, 1560 |
| BstNSI | RCATGY | 1 cut(s) | 1681 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 731 |
| BstPI | GGTNACC | 1 cut(s) | 869 |
| BstSCI | CCNGG | 9 cut(s) | 163, 319, 398, 399, 439, 592, 790, 832, 1558 |
| BstSLI | GKGCMC | 1 cut(s) | 1518 |
| BstUI | CGCG | 1 cut(s) | 758 |
| BstV1I | GCAGC | 8 cut(s) | 138, 667, 749, 758, 838, 1004, 1129, 1647 |
| BstV2I | GAAGAC | 2 cut(s) | 1281, 1612 |
| BstX2I | RGATCY | 2 cut(s) | 1171, 1486 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 833, 1427 |
| BstYI | RGATCY | 2 cut(s) | 1171, 1486 |
| BsuRI | GGCC | 3 cut(s) | 1410, 1532, 1550 |
| BtgI | CCRYGG | 1 cut(s) | 275 |
| BtsCI | GGATG | 4 cut(s) | 693, 1348, 1390, 1532 |
| BtsIMutI | CAGTG | 2 cut(s) | 244, 1576 |
| Cac8I | GCNNGC | 1 cut(s) | 381 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1511 |
| CfoI | GCGC | 1 cut(s) | 760 |
| Cfr13I | GGNCC | 8 cut(s) | 167, 279, 287, 396, 643, 1121, 1462, 1530 |
| Cfr9I | CCCGGG | 1 cut(s) | 399 |
| CseI | GACGC | 1 cut(s) | 752 |
| CsiI | ACCWGGT | 1 cut(s) | 832 |
| Csp6I | GTAC | 2 cut(s) | 53, 524 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1356, 1391 |
| CviAII | CATG | 9 cut(s) | 251, 276, 413, 685, 847, 1396, 1406, 1602, 1678 |
| CviQI | GTAC | 2 cut(s) | 53, 524 |
| DdeI | CTNAG | 3 cut(s) | 132, 706, 1496 |
| DpnI | GATC | 5 cut(s) | 344, 1173, 1339, 1389, 1488 |
| DpnII | GATC | 5 cut(s) | 342, 1171, 1337, 1387, 1486 |
| DraIII | CACNNNGTG | 1 cut(s) | 308 |
| EaeI | YGGCCR | 1 cut(s) | 1408 |
| Eam1104I | CTCTTC | 1 cut(s) | 62 |
| EarI | CTCTTC | 1 cut(s) | 62 |
| Eco130I | CCWWGG | 3 cut(s) | 275, 865, 1099 |
| Eco24I | GRGCYC | 1 cut(s) | 468 |
| Eco31I | GGTCTC | 1 cut(s) | 591 |
| Eco47I | GGWCC | 7 cut(s) | 167, 279, 287, 396, 643, 1121, 1462 |
| Eco57I | CTGAAG | 4 cut(s) | 14, 60, 495, 1293 |
| Eco88I | CYCGRG | 2 cut(s) | 399, 461 |
| Eco91I | GGTNACC | 1 cut(s) | 869 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1121, 1462 |
| EcoO65I | GGTNACC | 1 cut(s) | 869 |
| EcoRII | CCWGG | 6 cut(s) | 163, 439, 592, 790, 832, 1558 |
| EcoT14I | CCWWGG | 3 cut(s) | 275, 865, 1099 |
| EcoT22I | ATGCAT | 1 cut(s) | 1401 |
| EcoT38I | GRGCYC | 1 cut(s) | 468 |
| ErhI | CCWWGG | 3 cut(s) | 275, 865, 1099 |
| FaeI | CATG | 9 cut(s) | 254, 279, 416, 688, 850, 1399, 1409, 1605, 1681 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 354, 386 |
| FaqI | GGGAC | 4 cut(s) | 382, 955, 1144, 1322 |
| FatI | CATG | 9 cut(s) | 250, 275, 412, 684, 846, 1395, 1405, 1601, 1677 |
| FokI | GGATG | 4 cut(s) | 680, 1355, 1377, 1539 |
| FriOI | GRGCYC | 1 cut(s) | 468 |
| FspBI | CTAG | 5 cut(s) | 50, 447, 855, 875, 1025 |
| GlaI | GCGC | 1 cut(s) | 759 |
| GsaI | CCCAGC | 1 cut(s) | 1383 |
| GsuI | CTGGAG | 1 cut(s) | 1581 |
| HaeIII | GGCC | 3 cut(s) | 1410, 1532, 1550 |
| HapII | CCGG | 4 cut(s) | 320, 400, 546, 1551 |
| HgaI | GACGC | 1 cut(s) | 752 |
| HhaI | GCGC | 1 cut(s) | 760 |
| Hin1II | CATG | 9 cut(s) | 254, 279, 416, 688, 850, 1399, 1409, 1605, 1681 |
| Hin6I | GCGC | 1 cut(s) | 758 |
| HinP1I | GCGC | 1 cut(s) | 758 |
| HincII | GTYRAC | 1 cut(s) | 590 |
| HindII | GTYRAC | 1 cut(s) | 590 |
| HindIII | AAGCTT | 3 cut(s) | 72, 1162, 1194 |
| HinfI | GANTC | 6 cut(s) | 104, 255, 271, 655, 1132, 1542 |
| HpaII | CCGG | 4 cut(s) | 320, 400, 546, 1551 |
| HphI | GGTGA | 4 cut(s) | 506, 1093, 1330, 1586 |
| Hpy166II | GTNNAC | 4 cut(s) | 590, 643, 1084, 1456 |
| Hpy188I | TCNGA | 7 cut(s) | 283, 568, 622, 668, 1287, 1342, 1541 |
| Hpy188III | TCNNGA | 3 cut(s) | 290, 461, 1586 |
| Hpy8I | GTNNAC | 4 cut(s) | 590, 643, 1084, 1456 |
| Hpy99I | CGWCG | 1 cut(s) | 409 |
| HpyAV | CCTTC | 5 cut(s) | 71, 356, 977, 1112, 1315 |
| HpyCH4IV | ACGT | 2 cut(s) | 1591, 1655 |
| HpyCH4V | TGCA | 5 cut(s) | 100, 126, 992, 1399, 1612 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 32, 743, 746, 755, 857, 1020 |
| HpyF3I | CTNAG | 3 cut(s) | 132, 706, 1496 |
| HpySE526I | ACGT | 2 cut(s) | 1591, 1655 |
| Hsp92II | CATG | 9 cut(s) | 254, 279, 416, 688, 850, 1399, 1409, 1605, 1681 |
| HspAI | GCGC | 1 cut(s) | 758 |
| Kzo9I | GATC | 5 cut(s) | 342, 1171, 1337, 1387, 1486 |
| LmnI | GCTCC | 2 cut(s) | 416, 669 |
| Lsp1109I | GCAGC | 8 cut(s) | 138, 667, 749, 758, 838, 1004, 1129, 1647 |
| LweI | GCATC | 4 cut(s) | 559, 702, 796, 1408 |
| MabI | ACCWGGT | 1 cut(s) | 832 |
| MaeI | CTAG | 5 cut(s) | 50, 447, 855, 875, 1025 |
| MaeII | ACGT | 2 cut(s) | 1591, 1655 |
| MaeIII | GTNAC | 3 cut(s) | 869, 1507, 1592 |
| MalI | GATC | 5 cut(s) | 344, 1173, 1339, 1389, 1488 |
| MboI | GATC | 5 cut(s) | 342, 1171, 1337, 1387, 1486 |
| MboII | GAAGA | 6 cut(s) | 49, 352, 694, 1286, 1394, 1612 |
| MflI | RGATCY | 2 cut(s) | 1171, 1486 |
| MhlI | GDGCHC | 2 cut(s) | 468, 1518 |
| MlsI | TGGCCA | 1 cut(s) | 1410 |
| MluCI | AATT | 7 cut(s) | 88, 158, 420, 533, 1350, 1369, 1444 |
| MluNI | TGGCCA | 1 cut(s) | 1410 |
| MlyI | GAGTC | 3 cut(s) | 264, 649, 1126 |
| MmeI | TCCRAC | 1 cut(s) | 1408 |
| Mox20I | TGGCCA | 1 cut(s) | 1410 |
| Mph1103I | ATGCAT | 1 cut(s) | 1401 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1066 |
| MscI | TGGCCA | 1 cut(s) | 1410 |
| MseI | TTAA | 5 cut(s) | 150, 176, 1017, 1260, 1520 |
| MslI | CAYNNNNRTG | 4 cut(s) | 249, 1316, 1404, 1647 |
| Msp20I | TGGCCA | 1 cut(s) | 1410 |
| MspA1I | CMGCKG | 1 cut(s) | 1535 |
| MspCI | CTTAAG | 1 cut(s) | 1519 |
| MspI | CCGG | 4 cut(s) | 320, 400, 546, 1551 |
| MspR9I | CCNGG | 9 cut(s) | 165, 321, 400, 401, 441, 594, 792, 834, 1560 |
| Mva1269I | GAATGC | 2 cut(s) | 994, 1614 |
| MvaI | CCWGG | 6 cut(s) | 165, 441, 594, 792, 834, 1560 |
| MvnI | CGCG | 1 cut(s) | 758 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 32, 743, 746, 755, 857, 1020 |
| NciI | CCSGG | 3 cut(s) | 321, 400, 401 |
| NcoI | CCATGG | 1 cut(s) | 275 |
| NdeII | GATC | 5 cut(s) | 342, 1171, 1337, 1387, 1486 |
| NlaIII | CATG | 9 cut(s) | 254, 279, 416, 688, 850, 1399, 1409, 1605, 1681 |
| NlaIV | GGNNCC | 5 cut(s) | 367, 398, 1123, 1173, 1464 |
| NmuCI | GTSAC | 1 cut(s) | 1592 |
| NsiI | ATGCAT | 1 cut(s) | 1401 |
| NspI | RCATGY | 1 cut(s) | 1681 |
| OliI | CACNNNNGTG | 1 cut(s) | 1316 |
| PctI | GAATGC | 2 cut(s) | 994, 1614 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1066 |
| PfeI | GAWTC | 3 cut(s) | 104, 271, 1542 |
| PfoI | TCCNGGA | 3 cut(s) | 163, 790, 1558 |
| PleI | GAGTC | 3 cut(s) | 263, 649, 1126 |
| PpsI | GAGTC | 3 cut(s) | 263, 649, 1126 |
| PpuMI | RGGWCCY | 2 cut(s) | 1121, 1462 |
| PshAI | GACNNNNGTC | 1 cut(s) | 731 |
| PsiI | TTATAA | 2 cut(s) | 87, 186 |
| Psp1406I | AACGTT | 1 cut(s) | 1655 |
| Psp5II | RGGWCCY | 2 cut(s) | 1121, 1462 |
| Psp6I | CCWGG | 6 cut(s) | 163, 439, 592, 790, 832, 1558 |
| PspEI | GGTNACC | 1 cut(s) | 869 |
| PspFI | CCCAGC | 1 cut(s) | 1379 |
| PspGI | CCWGG | 6 cut(s) | 163, 439, 592, 790, 832, 1558 |
| PspN4I | GGNNCC | 5 cut(s) | 367, 398, 1123, 1173, 1464 |
| PspPI | GGNCC | 8 cut(s) | 167, 279, 287, 396, 643, 1121, 1462, 1530 |
| PspPPI | RGGWCCY | 2 cut(s) | 1121, 1462 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1511 |
| PsuI | RGATCY | 2 cut(s) | 1171, 1486 |
| RsaI | GTAC | 2 cut(s) | 54, 525 |
| RsaNI | GTAC | 2 cut(s) | 53, 524 |
| RseI | CAYNNNNRTG | 4 cut(s) | 249, 1316, 1404, 1647 |
| SaqAI | TTAA | 5 cut(s) | 150, 176, 1017, 1260, 1520 |
| Sau3AI | GATC | 5 cut(s) | 342, 1171, 1337, 1387, 1486 |
| Sau96I | GGNCC | 8 cut(s) | 167, 279, 287, 396, 643, 1121, 1462, 1530 |
| ScaI | AGTACT | 1 cut(s) | 54 |
| SchI | GAGTC | 3 cut(s) | 264, 649, 1126 |
| ScrFI | CCNGG | 9 cut(s) | 165, 321, 400, 401, 441, 594, 792, 834, 1560 |
| SduI | GDGCHC | 2 cut(s) | 468, 1518 |
| SexAI | ACCWGGT | 1 cut(s) | 832 |
| SfaNI | GCATC | 4 cut(s) | 559, 702, 796, 1408 |
| SinI | GGWCC | 7 cut(s) | 167, 279, 287, 396, 643, 1121, 1462 |
| SmaI | CCCGGG | 1 cut(s) | 401 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 249, 1316, 1404, 1647 |
| SmlI | CTYRAG | 1 cut(s) | 1519 |
| SmoI | CTYRAG | 1 cut(s) | 1519 |
| SpeI | ACTAGT | 1 cut(s) | 49 |
| Sse9I | AATT | 7 cut(s) | 88, 158, 420, 533, 1350, 1369, 1444 |
| SsiI | CCGC | 3 cut(s) | 756, 1533, 1682 |
| SspI | AATATT | 2 cut(s) | 141, 489 |
| SspMI | CTAG | 5 cut(s) | 50, 447, 855, 875, 1025 |
| StyD4I | CCNGG | 9 cut(s) | 163, 319, 398, 399, 439, 592, 790, 832, 1558 |
| StyI | CCWWGG | 3 cut(s) | 275, 865, 1099 |
| TaiI | ACGT | 2 cut(s) | 1594, 1658 |
| TaqI | TCGA | 2 cut(s) | 1060, 1135 |
| TasI | AATT | 7 cut(s) | 88, 158, 420, 533, 1350, 1369, 1444 |
| TatI | WGTACW | 1 cut(s) | 52 |
| TauI | GCSGC | 2 cut(s) | 1535, 1684 |
| TfiI | GAWTC | 3 cut(s) | 104, 271, 1542 |
| Tru1I | TTAA | 5 cut(s) | 150, 176, 1017, 1260, 1520 |
| Tru9I | TTAA | 5 cut(s) | 150, 176, 1017, 1260, 1520 |
| TscAI | CASTG | 2 cut(s) | 251, 1583 |
| TseFI | GTSAC | 1 cut(s) | 1592 |
| TseI | GCWGC | 8 cut(s) | 126, 680, 737, 746, 851, 992, 1142, 1660 |
| Tsp45I | GTSAC | 1 cut(s) | 1592 |
| TspDTI | ATGAA | 9 cut(s) | 53, 203, 401, 546, 701, 1166, 1394, 1612, 1665 |
| TspMI | CCCGGG | 1 cut(s) | 399 |
| TspRI | CASTG | 2 cut(s) | 251, 1583 |
| Vha464I | CTTAAG | 1 cut(s) | 1519 |
| VpaK11BI | GGWCC | 7 cut(s) | 167, 279, 287, 396, 643, 1121, 1462 |
| XapI | RAATTY | 3 cut(s) | 158, 533, 1369 |
| XceI | RCATGY | 1 cut(s) | 1681 |
| XmaI | CCCGGG | 1 cut(s) | 399 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1066 |
| XspI | CTAG | 5 cut(s) | 50, 447, 855, 875, 1025 |
| ZrmI | AGTACT | 1 cut(s) | 54 |
| Zsp2I | ATGCAT | 1 cut(s) | 1401 |