Lignin degradation and detoxification of lignin-derived products
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 84 |
| Acc36I | ACCTGC | 1 cut(s) | 1296 |
| Acc65I | GGTACC | 1 cut(s) | 362 |
| AccB1I | GGYRCC | 1 cut(s) | 362 |
| AccB7I | CCANNNNNTGG | 4 cut(s) | 15, 243, 372, 799 |
| AccBSI | CCGCTC | 1 cut(s) | 1867 |
| AccI | GTMKAC | 1 cut(s) | 1191 |
| AccII | CGCG | 2 cut(s) | 734, 1695 |
| AccIII | TCCGGA | 2 cut(s) | 286, 1843 |
| AciI | CCGC | 7 cut(s) | 543, 897, 1542, 1693, 1758, 1824, 1865 |
| AclWI | GGATC | 3 cut(s) | 685, 1490, 1659 |
| AcoI | YGGCCR | 1 cut(s) | 790 |
| AcsI | RAATTY | 3 cut(s) | 155, 530, 1453 |
| AcvI | CACGTG | 1 cut(s) | 398 |
| AfaI | GTAC | 5 cut(s) | 364, 522, 917, 1262, 1778 |
| AflII | CTTAAG | 1 cut(s) | 1013 |
| AflIII | ACRYGT | 1 cut(s) | 1263 |
| AjnI | CCWGG | 8 cut(s) | 316, 436, 589, 787, 829, 1104, 1567, 1725 |
| Alw21I | GWGCWC | 1 cut(s) | 1843 |
| Alw26I | GTCTC | 5 cut(s) | 540, 588, 1009, 1768, 1809 |
| AlwI | GGATC | 3 cut(s) | 685, 1490, 1659 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1288 |
| Ama87I | CYCGRG | 1 cut(s) | 1868 |
| Aor13HI | TCCGGA | 2 cut(s) | 286, 1843 |
| AoxI | GGCC | 4 cut(s) | 790, 1178, 1539, 1557 |
| ApeKI | GCWGC | 3 cut(s) | 123, 848, 1148 |
| ApoI | RAATTY | 3 cut(s) | 155, 530, 1453 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1029, 1061 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1072 |
| Asp718I | GGTACC | 1 cut(s) | 362 |
| AspLEI | GCGC | 1 cut(s) | 1697 |
| AsuC2I | CCSGG | 1 cut(s) | 162 |
| AsuHPI | GGTGA | 5 cut(s) | 226, 503, 1339, 1595, 1780 |
| AvaI | CYCGRG | 1 cut(s) | 1868 |
| AvaII | GGWCC | 8 cut(s) | 164, 284, 413, 640, 1127, 1137, 1285, 1471 |
| BalI | TGGCCA | 1 cut(s) | 792 |
| BanI | GGYRCC | 1 cut(s) | 362 |
| BanII | GRGCYC | 1 cut(s) | 1843 |
| BbrPI | CACGTG | 1 cut(s) | 398 |
| BbsI | GAAGAC | 1 cut(s) | 1222 |
| Bbv12I | GWGCWC | 1 cut(s) | 1843 |
| BbvI | GCAGC | 3 cut(s) | 135, 835, 1135 |
| BccI | CCATC | 2 cut(s) | 275, 1712 |
| BceAI | ACGGC | 1 cut(s) | 1745 |
| BciT130I | CCWGG | 8 cut(s) | 318, 438, 591, 789, 831, 1106, 1569, 1727 |
| BcnI | CCSGG | 1 cut(s) | 162 |
| BcoDI | GTCTC | 5 cut(s) | 540, 588, 1009, 1768, 1809 |
| BfaI | CTAG | 4 cut(s) | 444, 852, 1394, 1800 |
| BfmI | CTRYAG | 2 cut(s) | 351, 906 |
| BfrI | CTTAAG | 1 cut(s) | 1013 |
| BfuAI | ACCTGC | 1 cut(s) | 1296 |
| BisI | GCNGC | 4 cut(s) | 124, 849, 1149, 1542 |
| BlsI | GCNGC | 4 cut(s) | 125, 850, 1150, 1543 |
| Bme1390I | CCNGG | 9 cut(s) | 162, 318, 438, 591, 789, 831, 1106, 1569, 1727 |
| Bme18I | GGWCC | 8 cut(s) | 164, 284, 413, 640, 1127, 1137, 1285, 1471 |
| BmeT110I | CYCGRG | 1 cut(s) | 1868 |
| BmiI | GGNNCC | 6 cut(s) | 364, 1129, 1138, 1139, 1179, 1473 |
| BmrFI | CCNGG | 9 cut(s) | 162, 318, 438, 591, 789, 831, 1106, 1569, 1727 |
| BmsI | GCATC | 2 cut(s) | 793, 1417 |
| BpiI | GAAGAC | 1 cut(s) | 1222 |
| BpmI | CTGGAG | 2 cut(s) | 612, 1590 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1281 |
| BpuMI | CCSGG | 1 cut(s) | 162 |
| BsaAI | YACGTR | 2 cut(s) | 398, 1264 |
| BsaI | GGTCTC | 1 cut(s) | 588 |
| BsaJI | CCNNGG | 7 cut(s) | 263, 272, 317, 798, 1726, 1781, 1827 |
| BsaWI | WCCGGW | 2 cut(s) | 286, 1843 |
| Bse1I | ACTGG | 5 cut(s) | 20, 167, 248, 694, 1152 |
| BseAI | TCCGGA | 2 cut(s) | 286, 1843 |
| BseBI | CCWGG | 8 cut(s) | 318, 438, 591, 789, 831, 1106, 1569, 1727 |
| BseDI | CCNNGG | 7 cut(s) | 263, 272, 317, 798, 1726, 1781, 1827 |
| BseGI | GGATG | 3 cut(s) | 808, 1357, 1399 |
| BseMII | CTCAG | 1 cut(s) | 1295 |
| BseNI | ACTGG | 5 cut(s) | 20, 167, 248, 694, 1152 |
| BseRI | GAGGAG | 1 cut(s) | 201 |
| BseXI | GCAGC | 3 cut(s) | 135, 835, 1135 |
| BsgI | GTGCAG | 1 cut(s) | 142 |
| Bsh1236I | CGCG | 2 cut(s) | 734, 1695 |
| BshFI | GGCC | 4 cut(s) | 792, 1180, 1541, 1559 |
| BshNI | GGYRCC | 1 cut(s) | 362 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1843 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1868 |
| BsiSI | CCGG | 3 cut(s) | 162, 287, 1844 |
| BsiWI | CGTACG | 1 cut(s) | 1260 |
| BslFI | GGGAC | 5 cut(s) | 1031, 1150, 1333, 1360, 1508 |
| BsmAI | GTCTC | 5 cut(s) | 540, 588, 1009, 1768, 1809 |
| BsmBI | CGTCTC | 1 cut(s) | 1768 |
| BsmFI | GGGAC | 5 cut(s) | 1031, 1150, 1333, 1360, 1508 |
| BsnI | GGCC | 4 cut(s) | 792, 1180, 1541, 1559 |
| Bso31I | GGTCTC | 1 cut(s) | 588 |
| BsoBI | CYCGRG | 1 cut(s) | 1868 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1843 |
| Bsp13I | TCCGGA | 2 cut(s) | 286, 1843 |
| Bsp143I | GATC | 5 cut(s) | 339, 690, 1396, 1495, 1664 |
| Bsp19I | CCATGG | 3 cut(s) | 272, 798, 1781 |
| Bsp68I | TCGCGA | 1 cut(s) | 734 |
| BspACI | CCGC | 7 cut(s) | 543, 897, 1542, 1693, 1758, 1824, 1865 |
| BspANI | GGCC | 4 cut(s) | 792, 1180, 1541, 1559 |
| BspCNI | CTCAG | 1 cut(s) | 1294 |
| BspEI | TCCGGA | 2 cut(s) | 286, 1843 |
| BspFNI | CGCG | 2 cut(s) | 734, 1695 |
| BspHI | TCATGA | 1 cut(s) | 1851 |
| BspLI | GGNNCC | 6 cut(s) | 364, 1129, 1138, 1139, 1179, 1473 |
| BspMI | ACCTGC | 1 cut(s) | 1296 |
| BspPI | GGATC | 3 cut(s) | 685, 1490, 1659 |
| BspT107I | GGYRCC | 1 cut(s) | 362 |
| BspTI | CTTAAG | 1 cut(s) | 1013 |
| BspTNI | GGTCTC | 1 cut(s) | 588 |
| BsrBI | CCGCTC | 1 cut(s) | 1867 |
| BsrI | ACTGG | 5 cut(s) | 20, 167, 248, 694, 1152 |
| BssECI | CCNNGG | 7 cut(s) | 263, 272, 317, 798, 1726, 1781, 1827 |
| BssMI | GATC | 5 cut(s) | 339, 690, 1396, 1495, 1664 |
| BssT1I | CCWWGG | 4 cut(s) | 263, 272, 798, 1781 |
| Bst2UI | CCWGG | 8 cut(s) | 318, 438, 591, 789, 831, 1106, 1569, 1727 |
| Bst6I | CTCTTC | 1 cut(s) | 59 |
| BstAFI | CTTAAG | 1 cut(s) | 1013 |
| BstBAI | YACGTR | 2 cut(s) | 398, 1264 |
| BstC8I | GCNNGC | 3 cut(s) | 378, 745, 1711 |
| BstDEI | CTNAG | 4 cut(s) | 1008, 1281, 1381, 1505 |
| BstDSI | CCRYGG | 4 cut(s) | 272, 798, 1781, 1827 |
| BstF5I | GGATG | 3 cut(s) | 808, 1357, 1399 |
| BstFNI | CGCG | 2 cut(s) | 734, 1695 |
| BstHHI | GCGC | 1 cut(s) | 1697 |
| BstKTI | GATC | 5 cut(s) | 342, 693, 1399, 1498, 1667 |
| BstMAI | GTCTC | 5 cut(s) | 540, 588, 1009, 1768, 1809 |
| BstMBI | GATC | 5 cut(s) | 339, 690, 1396, 1495, 1664 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 566, 740, 854 |
| BstNI | CCWGG | 8 cut(s) | 318, 438, 591, 789, 831, 1106, 1569, 1727 |
| BstNSI | RCATGY | 1 cut(s) | 1046 |
| BstSCI | CCNGG | 9 cut(s) | 160, 316, 436, 589, 787, 829, 1104, 1567, 1725 |
| BstSFI | CTRYAG | 2 cut(s) | 351, 906 |
| BstUI | CGCG | 2 cut(s) | 734, 1695 |
| BstV1I | GCAGC | 3 cut(s) | 135, 835, 1135 |
| BstV2I | GAAGAC | 1 cut(s) | 1222 |
| BstX2I | RGATCY | 2 cut(s) | 690, 1664 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 830, 1436 |
| BstYI | RGATCY | 2 cut(s) | 690, 1664 |
| BsuRI | GGCC | 4 cut(s) | 792, 1180, 1541, 1559 |
| BtgI | CCRYGG | 4 cut(s) | 272, 798, 1781, 1827 |
| BtgZI | GCGATG | 2 cut(s) | 760, 1690 |
| BtsCI | GGATG | 3 cut(s) | 808, 1357, 1399 |
| BtsI | GCAGTG | 2 cut(s) | 1275, 1585 |
| BtsIMutI | CAGTG | 6 cut(s) | 241, 391, 870, 1275, 1512, 1585 |
| BtuMI | TCGCGA | 1 cut(s) | 734 |
| BveI | ACCTGC | 1 cut(s) | 1296 |
| Cac8I | GCNNGC | 3 cut(s) | 378, 745, 1711 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1288 |
| CciI | TCATGA | 1 cut(s) | 1851 |
| CfoI | GCGC | 1 cut(s) | 1697 |
| CseI | GACGC | 4 cut(s) | 77, 575, 749, 1589 |
| CsiI | ACCWGGT | 1 cut(s) | 829 |
| Csp6I | GTAC | 5 cut(s) | 363, 521, 916, 1261, 1777 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1365, 1400 |
| CviQI | GTAC | 5 cut(s) | 363, 521, 916, 1261, 1777 |
| DdeI | CTNAG | 4 cut(s) | 1008, 1281, 1381, 1505 |
| DpnI | GATC | 5 cut(s) | 341, 692, 1398, 1497, 1666 |
| DpnII | GATC | 5 cut(s) | 339, 690, 1396, 1495, 1664 |
| EaeI | YGGCCR | 1 cut(s) | 790 |
| Eam1104I | CTCTTC | 1 cut(s) | 59 |
| EarI | CTCTTC | 1 cut(s) | 59 |
| Ecl136II | GAGCTC | 1 cut(s) | 1841 |
| Eco130I | CCWWGG | 4 cut(s) | 263, 272, 798, 1781 |
| Eco24I | GRGCYC | 1 cut(s) | 1843 |
| Eco31I | GGTCTC | 1 cut(s) | 588 |
| Eco47I | GGWCC | 8 cut(s) | 164, 284, 413, 640, 1127, 1137, 1285, 1471 |
| Eco53kI | GAGCTC | 1 cut(s) | 1841 |
| Eco72I | CACGTG | 1 cut(s) | 398 |
| Eco88I | CYCGRG | 1 cut(s) | 1868 |
| EcoICRI | GAGCTC | 1 cut(s) | 1841 |
| EcoO109I | RGGNCCY | 4 cut(s) | 1127, 1137, 1285, 1471 |
| EcoRII | CCWGG | 8 cut(s) | 316, 436, 589, 787, 829, 1104, 1567, 1725 |
| EcoT14I | CCWWGG | 4 cut(s) | 263, 272, 798, 1781 |
| EcoT22I | ATGCAT | 1 cut(s) | 1410 |
| EcoT38I | GRGCYC | 1 cut(s) | 1843 |
| ErhI | CCWWGG | 4 cut(s) | 263, 272, 798, 1781 |
| Esp3I | CGTCTC | 1 cut(s) | 1768 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 641, 673 |
| FaqI | GGGAC | 5 cut(s) | 1031, 1150, 1333, 1360, 1508 |
| FauI | CCCGC | 1 cut(s) | 1765 |
| FblI | GTMKAC | 1 cut(s) | 1191 |
| Fnu4HI | GCNGC | 4 cut(s) | 124, 849, 1149, 1542 |
| FokI | GGATG | 3 cut(s) | 815, 1364, 1386 |
| FriOI | GRGCYC | 1 cut(s) | 1843 |
| Fsp4HI | GCNGC | 4 cut(s) | 124, 849, 1149, 1542 |
| FspBI | CTAG | 4 cut(s) | 444, 852, 1394, 1800 |
| GlaI | GCGC | 1 cut(s) | 1696 |
| GluI | GCNGC | 4 cut(s) | 124, 849, 1149, 1542 |
| GsuI | CTGGAG | 2 cut(s) | 612, 1590 |
| HaeIII | GGCC | 4 cut(s) | 792, 1180, 1541, 1559 |
| HapII | CCGG | 3 cut(s) | 162, 287, 1844 |
| HgaI | GACGC | 4 cut(s) | 77, 575, 749, 1589 |
| HhaI | GCGC | 1 cut(s) | 1697 |
| Hin6I | GCGC | 1 cut(s) | 1695 |
| HinP1I | GCGC | 1 cut(s) | 1695 |
| HincII | GTYRAC | 2 cut(s) | 587, 1771 |
| HindII | GTYRAC | 2 cut(s) | 587, 1771 |
| HindIII | AAGCTT | 3 cut(s) | 1010, 1168, 1203 |
| HinfI | GANTC | 7 cut(s) | 268, 877, 1227, 1250, 1551, 1795, 1805 |
| HpaII | CCGG | 3 cut(s) | 162, 287, 1844 |
| HphI | GGTGA | 5 cut(s) | 226, 503, 1339, 1595, 1780 |
| Hpy166II | GTNNAC | 4 cut(s) | 587, 640, 1192, 1771 |
| Hpy188I | TCNGA | 8 cut(s) | 280, 565, 619, 665, 1296, 1550, 1814, 1876 |
| Hpy188III | TCNNGA | 9 cut(s) | 65, 94, 287, 733, 817, 1247, 1283, 1844, 1852 |
| Hpy8I | GTNNAC | 4 cut(s) | 587, 640, 1192, 1771 |
| HpyAV | CCTTC | 5 cut(s) | 353, 974, 982, 1318, 1651 |
| HpyCH4IV | ACGT | 2 cut(s) | 397, 1263 |
| HpyCH4V | TGCA | 4 cut(s) | 123, 1303, 1408, 1585 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 566, 740, 854 |
| HpyF3I | CTNAG | 4 cut(s) | 1008, 1281, 1381, 1505 |
| HpySE526I | ACGT | 2 cut(s) | 397, 1263 |
| HspAI | GCGC | 1 cut(s) | 1695 |
| KflI | GGGWCCC | 1 cut(s) | 1137 |
| Kpn2I | TCCGGA | 2 cut(s) | 286, 1843 |
| KpnI | GGTACC | 1 cut(s) | 366 |
| Kzo9I | GATC | 5 cut(s) | 339, 690, 1396, 1495, 1664 |
| LmnI | GCTCC | 4 cut(s) | 666, 1597, 1838, 1846 |
| Lsp1109I | GCAGC | 3 cut(s) | 135, 835, 1135 |
| LweI | GCATC | 2 cut(s) | 793, 1417 |
| MabI | ACCWGGT | 1 cut(s) | 829 |
| MaeI | CTAG | 4 cut(s) | 444, 852, 1394, 1800 |
| MaeII | ACGT | 2 cut(s) | 397, 1263 |
| MaeIII | GTNAC | 4 cut(s) | 65, 393, 814, 1601 |
| MalI | GATC | 5 cut(s) | 341, 692, 1398, 1497, 1666 |
| MbiI | CCGCTC | 1 cut(s) | 1867 |
| MboI | GATC | 5 cut(s) | 339, 690, 1396, 1495, 1664 |
| MboII | GAAGA | 7 cut(s) | 46, 368, 1222, 1342, 1403, 1475, 1651 |
| MfeI | CAATTG | 1 cut(s) | 555 |
| MflI | RGATCY | 2 cut(s) | 690, 1664 |
| MhlI | GDGCHC | 1 cut(s) | 1843 |
| MlsI | TGGCCA | 1 cut(s) | 792 |
| MluCI | AATT | 8 cut(s) | 85, 155, 530, 555, 1071, 1270, 1440, 1453 |
| MluNI | TGGCCA | 1 cut(s) | 792 |
| MlyI | GAGTC | 2 cut(s) | 1236, 1799 |
| MmeI | TCCRAC | 2 cut(s) | 1417, 1854 |
| Mox20I | TGGCCA | 1 cut(s) | 792 |
| Mph1103I | ATGCAT | 1 cut(s) | 1410 |
| MroI | TCCGGA | 2 cut(s) | 286, 1843 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1072 |
| MscI | TGGCCA | 1 cut(s) | 792 |
| MseI | TTAA | 4 cut(s) | 147, 1014, 1029, 1269 |
| MslI | CAYNNNNRTG | 4 cut(s) | 246, 704, 1413, 1419 |
| Msp20I | TGGCCA | 1 cut(s) | 792 |
| MspCI | CTTAAG | 1 cut(s) | 1013 |
| MspI | CCGG | 3 cut(s) | 162, 287, 1844 |
| MspR9I | CCNGG | 9 cut(s) | 162, 318, 438, 591, 789, 831, 1106, 1569, 1727 |
| MunI | CAATTG | 1 cut(s) | 555 |
| MvaI | CCWGG | 8 cut(s) | 318, 438, 591, 789, 831, 1106, 1569, 1727 |
| MvnI | CGCG | 2 cut(s) | 734, 1695 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 566, 740, 854 |
| NciI | CCSGG | 1 cut(s) | 162 |
| NcoI | CCATGG | 3 cut(s) | 272, 798, 1781 |
| NdeII | GATC | 5 cut(s) | 339, 690, 1396, 1495, 1664 |
| NlaIV | GGNNCC | 6 cut(s) | 364, 1129, 1138, 1139, 1179, 1473 |
| NmuCI | GTSAC | 4 cut(s) | 65, 393, 814, 1601 |
| NruI | TCGCGA | 1 cut(s) | 734 |
| NsiI | ATGCAT | 1 cut(s) | 1410 |
| NspI | RCATGY | 1 cut(s) | 1046 |
| PagI | TCATGA | 1 cut(s) | 1851 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 731, 1875 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1072 |
| PfeI | GAWTC | 5 cut(s) | 268, 877, 1250, 1551, 1795 |
| Pfl23II | CGTACG | 1 cut(s) | 1260 |
| PflMI | CCANNNNNTGG | 4 cut(s) | 15, 243, 372, 799 |
| PfoI | TCCNGGA | 2 cut(s) | 160, 1567 |
| PkrI | GCNGC | 4 cut(s) | 125, 850, 1150, 1543 |
| PleI | GAGTC | 2 cut(s) | 1235, 1799 |
| PmaCI | CACGTG | 1 cut(s) | 398 |
| PmlI | CACGTG | 1 cut(s) | 398 |
| PpsI | GAGTC | 2 cut(s) | 1235, 1799 |
| Ppu21I | YACGTR | 2 cut(s) | 398, 1264 |
| PpuMI | RGGWCCY | 4 cut(s) | 1127, 1137, 1285, 1471 |
| PsiI | TTATAA | 1 cut(s) | 84 |
| Psp124BI | GAGCTC | 1 cut(s) | 1843 |
| Psp5II | RGGWCCY | 4 cut(s) | 1127, 1137, 1285, 1471 |
| Psp6I | CCWGG | 8 cut(s) | 316, 436, 589, 787, 829, 1104, 1567, 1725 |
| PspCI | CACGTG | 1 cut(s) | 398 |
| PspGI | CCWGG | 8 cut(s) | 316, 436, 589, 787, 829, 1104, 1567, 1725 |
| PspLI | CGTACG | 1 cut(s) | 1260 |
| PspN4I | GGNNCC | 6 cut(s) | 364, 1129, 1138, 1139, 1179, 1473 |
| PspPPI | RGGWCCY | 4 cut(s) | 1127, 1137, 1285, 1471 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1288 |
| PsuI | RGATCY | 2 cut(s) | 690, 1664 |
| RruI | TCGCGA | 1 cut(s) | 734 |
| RsaI | GTAC | 5 cut(s) | 364, 522, 917, 1262, 1778 |
| RsaNI | GTAC | 5 cut(s) | 363, 521, 916, 1261, 1777 |
| RseI | CAYNNNNRTG | 4 cut(s) | 246, 704, 1413, 1419 |
| SacI | GAGCTC | 1 cut(s) | 1843 |
| SaqAI | TTAA | 4 cut(s) | 147, 1014, 1029, 1269 |
| SatI | GCNGC | 4 cut(s) | 124, 849, 1149, 1542 |
| Sau3AI | GATC | 5 cut(s) | 339, 690, 1396, 1495, 1664 |
| SchI | GAGTC | 2 cut(s) | 1236, 1799 |
| ScrFI | CCNGG | 9 cut(s) | 162, 318, 438, 591, 789, 831, 1106, 1569, 1727 |
| SduI | GDGCHC | 1 cut(s) | 1843 |
| SexAI | ACCWGGT | 1 cut(s) | 829 |
| SfaNI | GCATC | 2 cut(s) | 793, 1417 |
| SfcI | CTRYAG | 2 cut(s) | 351, 906 |
| SinI | GGWCC | 8 cut(s) | 164, 284, 413, 640, 1127, 1137, 1285, 1471 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 246, 704, 1413, 1419 |
| SmlI | CTYRAG | 1 cut(s) | 1013 |
| SmoI | CTYRAG | 1 cut(s) | 1013 |
| Sse9I | AATT | 8 cut(s) | 85, 155, 530, 555, 1071, 1270, 1440, 1453 |
| SsiI | CCGC | 7 cut(s) | 543, 897, 1542, 1693, 1758, 1824, 1865 |
| SspI | AATATT | 1 cut(s) | 486 |
| SspMI | CTAG | 4 cut(s) | 444, 852, 1394, 1800 |
| SstI | GAGCTC | 1 cut(s) | 1843 |
| StyD4I | CCNGG | 9 cut(s) | 160, 316, 436, 589, 787, 829, 1104, 1567, 1725 |
| StyI | CCWWGG | 4 cut(s) | 263, 272, 798, 1781 |
| TaiI | ACGT | 2 cut(s) | 400, 1266 |
| TaqI | TCGA | 3 cut(s) | 762, 885, 1066 |
| TasI | AATT | 8 cut(s) | 85, 155, 530, 555, 1071, 1270, 1440, 1453 |
| TatI | WGTACW | 1 cut(s) | 915 |
| TauI | GCSGC | 1 cut(s) | 1544 |
| TfiI | GAWTC | 5 cut(s) | 268, 877, 1250, 1551, 1795 |
| Tru1I | TTAA | 4 cut(s) | 147, 1014, 1029, 1269 |
| Tru9I | TTAA | 4 cut(s) | 147, 1014, 1029, 1269 |
| TscAI | CASTG | 6 cut(s) | 248, 391, 877, 1282, 1519, 1592 |
| TseFI | GTSAC | 4 cut(s) | 65, 393, 814, 1601 |
| TseI | GCWGC | 3 cut(s) | 123, 848, 1148 |
| Tsp45I | GTSAC | 4 cut(s) | 65, 393, 814, 1601 |
| TspDTI | ATGAA | 9 cut(s) | 50, 200, 398, 543, 688, 698, 1172, 1403, 1476 |
| TspGWI | ACGGA | 4 cut(s) | 231, 782, 1364, 1756 |
| TspRI | CASTG | 6 cut(s) | 248, 391, 877, 1282, 1519, 1592 |
| Van91I | CCANNNNNTGG | 4 cut(s) | 15, 243, 372, 799 |
| Vha464I | CTTAAG | 1 cut(s) | 1013 |
| VpaK11BI | GGWCC | 8 cut(s) | 164, 284, 413, 640, 1127, 1137, 1285, 1471 |
| XapI | RAATTY | 3 cut(s) | 155, 530, 1453 |
| XceI | RCATGY | 1 cut(s) | 1046 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 270 |
| XmiI | GTMKAC | 1 cut(s) | 1191 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1072 |
| XspI | CTAG | 4 cut(s) | 444, 852, 1394, 1800 |
| Zsp2I | ATGCAT | 1 cut(s) | 1410 |