Lignin degradation and detoxification of lignin-derived products
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 267 |
| AccB1I | GGYRCC | 1 cut(s) | 422 |
| AccII | CGCG | 1 cut(s) | 942 |
| AccIII | TCCGGA | 1 cut(s) | 551 |
| AciI | CCGC | 6 cut(s) | 576, 746, 858, 942, 1179, 1319 |
| AclWI | GGATC | 3 cut(s) | 411, 1392, 1517 |
| AcoI | YGGCCR | 2 cut(s) | 80, 855 |
| AcsI | RAATTY | 1 cut(s) | 1276 |
| AcuI | CTGAAG | 1 cut(s) | 1313 |
| AdeI | CACNNNGTG | 1 cut(s) | 312 |
| AfaI | GTAC | 4 cut(s) | 185, 417, 1325, 1370 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 445, 1155, 1343, 1559 |
| AflIII | ACRYGT | 1 cut(s) | 1169 |
| AgsI | TTSAA | 6 cut(s) | 323, 548, 637, 975, 1397, 1507 |
| AjiI | CACGTC | 1 cut(s) | 1585 |
| AjnI | CCWGG | 2 cut(s) | 325, 796 |
| AleI | CACNNNNGTG | 2 cut(s) | 98, 1379 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1255, 1287 |
| AluBI | AGCT | 8 cut(s) | 77, 86, 529, 756, 1050, 1055, 1200, 1232 |
| AluI | AGCT | 8 cut(s) | 77, 86, 529, 756, 1050, 1055, 1200, 1232 |
| Alw21I | GWGCWC | 1 cut(s) | 696 |
| Alw26I | GTCTC | 4 cut(s) | 192, 623, 1046, 1120 |
| Alw44I | GTGCAC | 1 cut(s) | 692 |
| AlwI | GGATC | 3 cut(s) | 411, 1392, 1517 |
| Aor13HI | TCCGGA | 1 cut(s) | 551 |
| AoxI | GGCC | 5 cut(s) | 80, 855, 1157, 1283, 1612 |
| ApaI | GGGCCC | 1 cut(s) | 1616 |
| ApaLI | GTGCAC | 1 cut(s) | 692 |
| ApoI | RAATTY | 1 cut(s) | 1276 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 929, 961 |
| AseI | ATTAAT | 1 cut(s) | 51 |
| AspLEI | GCGC | 2 cut(s) | 268, 1154 |
| AspS9I | GGNCC | 9 cut(s) | 173, 293, 559, 1157, 1207, 1284, 1498, 1612, 1613 |
| AsuC2I | CCSGG | 3 cut(s) | 171, 600, 1156 |
| AsuHPI | GGTGA | 4 cut(s) | 115, 331, 786, 1366 |
| AsuNHI | GCTAGC | 2 cut(s) | 752, 1181 |
| AvaII | GGWCC | 5 cut(s) | 173, 293, 559, 1207, 1498 |
| BaeGI | GKGCMC | 3 cut(s) | 317, 696, 1616 |
| BalI | TGGCCA | 1 cut(s) | 82 |
| BanI | GGYRCC | 1 cut(s) | 422 |
| BanII | GRGCYC | 1 cut(s) | 1616 |
| BauI | CACGAG | 1 cut(s) | 695 |
| Bbv12I | GWGCWC | 1 cut(s) | 696 |
| BccI | CCATC | 5 cut(s) | 434, 637, 722, 1127, 1434 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 35 |
| BciT130I | CCWGG | 2 cut(s) | 327, 798 |
| BcnI | CCSGG | 3 cut(s) | 171, 600, 1156 |
| BcoDI | GTCTC | 4 cut(s) | 192, 623, 1046, 1120 |
| BfaI | CTAG | 4 cut(s) | 753, 872, 1182, 1212 |
| BfmI | CTRYAG | 1 cut(s) | 861 |
| BisI | GCNGC | 3 cut(s) | 858, 1179, 1319 |
| BlpI | GCTNAGC | 1 cut(s) | 1051 |
| BlsI | GCNGC | 3 cut(s) | 859, 1180, 1320 |
| Bme1390I | CCNGG | 5 cut(s) | 171, 327, 600, 798, 1156 |
| Bme18I | GGWCC | 5 cut(s) | 173, 293, 559, 1207, 1498 |
| BmgBI | CACGTC | 1 cut(s) | 1585 |
| BmgT120I | GGNCC | 9 cut(s) | 173, 293, 559, 1157, 1207, 1284, 1498, 1612, 1613 |
| BmiI | GGNNCC | 8 cut(s) | 331, 424, 560, 561, 894, 1209, 1500, 1614 |
| BmrFI | CCNGG | 5 cut(s) | 171, 327, 600, 798, 1156 |
| BmsI | GCATC | 4 cut(s) | 66, 739, 1028, 1444 |
| BmtI | GCTAGC | 2 cut(s) | 756, 1185 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1051 |
| BpuEI | CTTGAG | 1 cut(s) | 1602 |
| BpuMI | CCSGG | 3 cut(s) | 171, 600, 1156 |
| BsaBI | GATNNNNATC | 1 cut(s) | 609 |
| BsaJI | CCNNGG | 4 cut(s) | 272, 281, 363, 1377 |
| BsaWI | WCCGGW | 2 cut(s) | 551, 1642 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 189, 219, 1100, 1130 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 445, 1155, 1343, 1559 |
| Bse1I | ACTGG | 1 cut(s) | 257 |
| Bse3DI | GCAATG | 1 cut(s) | 67 |
| Bse8I | GATNNNNATC | 1 cut(s) | 609 |
| BseAI | TCCGGA | 1 cut(s) | 551 |
| BseBI | CCWGG | 2 cut(s) | 327, 798 |
| BseDI | CCNNGG | 4 cut(s) | 272, 281, 363, 1377 |
| BseGI | GGATG | 3 cut(s) | 57, 754, 1426 |
| BseJI | GATNNNNATC | 1 cut(s) | 609 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 445, 1155, 1343, 1559 |
| BseMI | GCAATG | 1 cut(s) | 67 |
| BseMII | CTCAG | 2 cut(s) | 662, 1057 |
| BseNI | ACTGG | 1 cut(s) | 257 |
| BseRI | GAGGAG | 2 cut(s) | 53, 1518 |
| BseSI | GKGCMC | 3 cut(s) | 317, 696, 1616 |
| BseYI | CCCAGC | 1 cut(s) | 881 |
| Bsh1236I | CGCG | 1 cut(s) | 942 |
| BshFI | GGCC | 5 cut(s) | 82, 857, 1159, 1285, 1614 |
| BshNI | GGYRCC | 1 cut(s) | 422 |
| BsiHKAI | GWGCWC | 1 cut(s) | 696 |
| BsiSI | CCGG | 7 cut(s) | 171, 552, 600, 1035, 1155, 1282, 1643 |
| BslFI | GGGAC | 5 cut(s) | 572, 1180, 1360, 1414, 1535 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 445, 1155, 1343, 1559 |
| BsmAI | GTCTC | 4 cut(s) | 192, 623, 1046, 1120 |
| BsmFI | GGGAC | 5 cut(s) | 572, 1180, 1360, 1414, 1535 |
| BsnI | GGCC | 5 cut(s) | 82, 857, 1159, 1285, 1614 |
| Bsp120I | GGGCCC | 1 cut(s) | 1612 |
| Bsp1286I | GDGCHC | 3 cut(s) | 317, 696, 1616 |
| Bsp13I | TCCGGA | 1 cut(s) | 551 |
| Bsp1407I | TGTACA | 1 cut(s) | 415 |
| Bsp143I | GATC | 6 cut(s) | 403, 489, 604, 789, 1384, 1522 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1051 |
| Bsp19I | CCATGG | 1 cut(s) | 281 |
| BspACI | CCGC | 6 cut(s) | 576, 746, 858, 942, 1179, 1319 |
| BspANI | GGCC | 5 cut(s) | 82, 857, 1159, 1285, 1614 |
| BspCNI | CTCAG | 2 cut(s) | 663, 1056 |
| BspEI | TCCGGA | 1 cut(s) | 551 |
| BspFNI | CGCG | 1 cut(s) | 942 |
| BspLI | GGNNCC | 8 cut(s) | 331, 424, 560, 561, 894, 1209, 1500, 1614 |
| BspOI | GCTAGC | 2 cut(s) | 756, 1185 |
| BspPI | GGATC | 3 cut(s) | 411, 1392, 1517 |
| BspT107I | GGYRCC | 1 cut(s) | 422 |
| BsrDI | GCAATG | 1 cut(s) | 67 |
| BsrGI | TGTACA | 1 cut(s) | 415 |
| BsrI | ACTGG | 1 cut(s) | 257 |
| BssECI | CCNNGG | 4 cut(s) | 272, 281, 363, 1377 |
| BssMI | GATC | 6 cut(s) | 403, 489, 604, 789, 1384, 1522 |
| BssSI | CACGAG | 1 cut(s) | 695 |
| BssT1I | CCWWGG | 2 cut(s) | 272, 281 |
| Bst2BI | CACGAG | 1 cut(s) | 695 |
| Bst2UI | CCWGG | 2 cut(s) | 327, 798 |
| Bst6I | CTCTTC | 3 cut(s) | 549, 999, 1536 |
| BstAUI | TGTACA | 1 cut(s) | 415 |
| BstC8I | GCNNGC | 6 cut(s) | 20, 84, 190, 387, 754, 1183 |
| BstDEI | CTNAG | 3 cut(s) | 671, 1043, 1051 |
| BstDSI | CCRYGG | 2 cut(s) | 281, 1377 |
| BstF5I | GGATG | 3 cut(s) | 57, 754, 1426 |
| BstFNI | CGCG | 1 cut(s) | 942 |
| BstHHI | GCGC | 2 cut(s) | 268, 1154 |
| BstKTI | GATC | 6 cut(s) | 406, 492, 607, 792, 1387, 1525 |
| BstMAI | GTCTC | 4 cut(s) | 192, 623, 1046, 1120 |
| BstMBI | GATC | 6 cut(s) | 403, 489, 604, 789, 1384, 1522 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 83, 863 |
| BstNI | CCWGG | 2 cut(s) | 327, 798 |
| BstNSI | RCATGY | 1 cut(s) | 389 |
| BstSCI | CCNGG | 5 cut(s) | 169, 325, 598, 796, 1154 |
| BstSFI | CTRYAG | 1 cut(s) | 861 |
| BstSLI | GKGCMC | 3 cut(s) | 317, 696, 1616 |
| BstUI | CGCG | 1 cut(s) | 942 |
| BstX2I | RGATCY | 1 cut(s) | 1384 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 257 |
| BstYI | RGATCY | 1 cut(s) | 1384 |
| BsuRI | GGCC | 5 cut(s) | 82, 857, 1159, 1285, 1614 |
| BtgI | CCRYGG | 2 cut(s) | 281, 1377 |
| BtgZI | GCGATG | 1 cut(s) | 700 |
| BtrI | CACGTC | 1 cut(s) | 1585 |
| BtsCI | GGATG | 3 cut(s) | 57, 754, 1426 |
| BtsI | GCAGTG | 2 cut(s) | 317, 822 |
| BtsIMutI | CAGTG | 5 cut(s) | 250, 317, 400, 411, 822 |
| Cac8I | GCNNGC | 6 cut(s) | 20, 84, 190, 387, 754, 1183 |
| CfoI | GCGC | 2 cut(s) | 268, 1154 |
| Cfr13I | GGNCC | 9 cut(s) | 173, 293, 559, 1157, 1207, 1284, 1498, 1612, 1613 |
| CpoI | CGGWCCG | 1 cut(s) | 173 |
| CsiI | ACCWGGT | 1 cut(s) | 796 |
| Csp6I | GTAC | 4 cut(s) | 184, 416, 1324, 1369 |
| CspI | CGGWCCG | 1 cut(s) | 173 |
| CviQI | GTAC | 4 cut(s) | 184, 416, 1324, 1369 |
| DdeI | CTNAG | 3 cut(s) | 671, 1043, 1051 |
| DpnI | GATC | 6 cut(s) | 405, 491, 606, 791, 1386, 1524 |
| DpnII | GATC | 6 cut(s) | 403, 489, 604, 789, 1384, 1522 |
| DraIII | CACNNNGTG | 1 cut(s) | 312 |
| EaeI | YGGCCR | 2 cut(s) | 80, 855 |
| Eam1104I | CTCTTC | 3 cut(s) | 549, 999, 1536 |
| EarI | CTCTTC | 3 cut(s) | 549, 999, 1536 |
| EciI | GGCGGA | 1 cut(s) | 761 |
| Eco130I | CCWWGG | 2 cut(s) | 272, 281 |
| Eco24I | GRGCYC | 1 cut(s) | 1616 |
| Eco47I | GGWCC | 5 cut(s) | 173, 293, 559, 1207, 1498 |
| Eco57I | CTGAAG | 1 cut(s) | 1313 |
| EcoO109I | RGGNCCY | 4 cut(s) | 559, 1498, 1612, 1613 |
| EcoRII | CCWGG | 2 cut(s) | 325, 796 |
| EcoT14I | CCWWGG | 2 cut(s) | 272, 281 |
| EcoT22I | ATGCAT | 2 cut(s) | 1021, 1437 |
| EcoT38I | GRGCYC | 1 cut(s) | 1616 |
| ErhI | CCWWGG | 2 cut(s) | 272, 281 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 360, 392 |
| FaqI | GGGAC | 5 cut(s) | 572, 1180, 1360, 1414, 1535 |
| FauI | CCCGC | 1 cut(s) | 935 |
| FauNDI | CATATG | 1 cut(s) | 1566 |
| Fnu4HI | GCNGC | 3 cut(s) | 858, 1179, 1319 |
| FokI | GGATG | 3 cut(s) | 44, 761, 1413 |
| FriOI | GRGCYC | 1 cut(s) | 1616 |
| Fsp4HI | GCNGC | 3 cut(s) | 858, 1179, 1319 |
| FspBI | CTAG | 4 cut(s) | 753, 872, 1182, 1212 |
| FspI | TGCGCA | 1 cut(s) | 267 |
| GlaI | GCGC | 2 cut(s) | 267, 1153 |
| GluI | GCNGC | 3 cut(s) | 858, 1179, 1319 |
| GsaI | CCCAGC | 1 cut(s) | 885 |
| HaeIII | GGCC | 5 cut(s) | 82, 857, 1159, 1285, 1614 |
| HapII | CCGG | 7 cut(s) | 171, 552, 600, 1035, 1155, 1282, 1643 |
| HhaI | GCGC | 2 cut(s) | 268, 1154 |
| Hin6I | GCGC | 2 cut(s) | 266, 1152 |
| HinP1I | GCGC | 2 cut(s) | 266, 1152 |
| HincII | GTYRAC | 3 cut(s) | 596, 649, 1249 |
| HindII | GTYRAC | 3 cut(s) | 596, 649, 1249 |
| HindIII | AAGCTT | 4 cut(s) | 75, 527, 1048, 1230 |
| HinfI | GANTC | 4 cut(s) | 277, 319, 623, 1333 |
| HpaII | CCGG | 7 cut(s) | 171, 552, 600, 1035, 1155, 1282, 1643 |
| HphI | GGTGA | 4 cut(s) | 115, 331, 786, 1366 |
| Hpy166II | GTNNAC | 6 cut(s) | 596, 649, 694, 1189, 1207, 1249 |
| Hpy188I | TCNGA | 9 cut(s) | 289, 622, 628, 642, 834, 1046, 1332, 1338, 1536 |
| Hpy188III | TCNNGA | 3 cut(s) | 487, 552, 1619 |
| Hpy8I | GTNNAC | 6 cut(s) | 596, 649, 694, 1189, 1207, 1249 |
| Hpy99I | CGWCG | 1 cut(s) | 215 |
| HpyAV | CCTTC | 7 cut(s) | 362, 505, 978, 990, 1303, 1337, 1351 |
| HpyCH4IV | ACGT | 2 cut(s) | 1171, 1584 |
| HpyCH4V | TGCA | 7 cut(s) | 22, 479, 694, 928, 1019, 1435, 1441 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 83, 863 |
| HpyF3I | CTNAG | 3 cut(s) | 671, 1043, 1051 |
| HpySE526I | ACGT | 2 cut(s) | 1171, 1584 |
| HspAI | GCGC | 2 cut(s) | 266, 1152 |
| KflI | GGGWCCC | 1 cut(s) | 559 |
| Kpn2I | TCCGGA | 1 cut(s) | 551 |
| Kzo9I | GATC | 6 cut(s) | 403, 489, 604, 789, 1384, 1522 |
| LmnI | GCTCC | 1 cut(s) | 329 |
| LweI | GCATC | 4 cut(s) | 66, 739, 1028, 1444 |
| MabI | ACCWGGT | 1 cut(s) | 796 |
| MaeI | CTAG | 4 cut(s) | 753, 872, 1182, 1212 |
| MaeII | ACGT | 2 cut(s) | 1171, 1584 |
| MaeIII | GTNAC | 6 cut(s) | 89, 236, 730, 1445, 1585, 1629 |
| MalI | GATC | 6 cut(s) | 405, 491, 606, 791, 1386, 1524 |
| MboI | GATC | 6 cut(s) | 403, 489, 604, 789, 1384, 1522 |
| MboII | GAAGA | 6 cut(s) | 16, 527, 536, 963, 986, 1523 |
| MflI | RGATCY | 1 cut(s) | 1384 |
| MhlI | GDGCHC | 3 cut(s) | 317, 696, 1616 |
| MlsI | TGGCCA | 1 cut(s) | 82 |
| MluNI | TGGCCA | 1 cut(s) | 82 |
| MmeI | TCCRAC | 2 cut(s) | 267, 1444 |
| Mox20I | TGGCCA | 1 cut(s) | 82 |
| Mph1103I | ATGCAT | 2 cut(s) | 1021, 1437 |
| MroI | TCCGGA | 1 cut(s) | 551 |
| MscI | TGGCCA | 1 cut(s) | 82 |
| MseI | TTAA | 7 cut(s) | 51, 222, 588, 1026, 1086, 1296, 1660 |
| MslI | CAYNNNNRTG | 5 cut(s) | 98, 255, 1379, 1440, 1583 |
| Msp20I | TGGCCA | 1 cut(s) | 82 |
| MspA1I | CMGCKG | 1 cut(s) | 1321 |
| MspI | CCGG | 7 cut(s) | 171, 552, 600, 1035, 1155, 1282, 1643 |
| MspR9I | CCNGG | 5 cut(s) | 171, 327, 600, 798, 1156 |
| MvaI | CCWGG | 2 cut(s) | 327, 798 |
| MvnI | CGCG | 1 cut(s) | 942 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 83, 863 |
| NciI | CCSGG | 3 cut(s) | 171, 600, 1156 |
| NcoI | CCATGG | 1 cut(s) | 281 |
| NdeI | CATATG | 1 cut(s) | 1566 |
| NdeII | GATC | 6 cut(s) | 403, 489, 604, 789, 1384, 1522 |
| NheI | GCTAGC | 2 cut(s) | 752, 1181 |
| NlaIV | GGNNCC | 8 cut(s) | 331, 424, 560, 561, 894, 1209, 1500, 1614 |
| NmuCI | GTSAC | 2 cut(s) | 730, 1585 |
| NsbI | TGCGCA | 1 cut(s) | 267 |
| NsiI | ATGCAT | 2 cut(s) | 1021, 1437 |
| NspI | RCATGY | 1 cut(s) | 389 |
| OliI | CACNNNNGTG | 2 cut(s) | 98, 1379 |
| PaeI | GCATGC | 1 cut(s) | 389 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 314, 1182 |
| PfeI | GAWTC | 4 cut(s) | 277, 319, 623, 1333 |
| PflFI | GACNNNGTC | 1 cut(s) | 203 |
| PfoI | TCCNGGA | 1 cut(s) | 169 |
| PkrI | GCNGC | 3 cut(s) | 859, 1180, 1320 |
| PpuMI | RGGWCCY | 2 cut(s) | 559, 1498 |
| PshBI | ATTAAT | 1 cut(s) | 51 |
| Psp5II | RGGWCCY | 2 cut(s) | 559, 1498 |
| Psp6I | CCWGG | 2 cut(s) | 325, 796 |
| PspFI | CCCAGC | 1 cut(s) | 881 |
| PspGI | CCWGG | 2 cut(s) | 325, 796 |
| PspN4I | GGNNCC | 8 cut(s) | 331, 424, 560, 561, 894, 1209, 1500, 1614 |
| PspOMI | GGGCCC | 1 cut(s) | 1612 |
| PspPI | GGNCC | 9 cut(s) | 173, 293, 559, 1157, 1207, 1284, 1498, 1612, 1613 |
| PspPPI | RGGWCCY | 2 cut(s) | 559, 1498 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1614, 1646 |
| PsuI | RGATCY | 1 cut(s) | 1384 |
| PsyI | GACNNNGTC | 1 cut(s) | 203 |
| RsaI | GTAC | 4 cut(s) | 185, 417, 1325, 1370 |
| RsaNI | GTAC | 4 cut(s) | 184, 416, 1324, 1369 |
| RseI | CAYNNNNRTG | 5 cut(s) | 98, 255, 1379, 1440, 1583 |
| Rsr2I | CGGWCCG | 1 cut(s) | 173 |
| RsrII | CGGWCCG | 1 cut(s) | 173 |
| SaqAI | TTAA | 7 cut(s) | 51, 222, 588, 1026, 1086, 1296, 1660 |
| SatI | GCNGC | 3 cut(s) | 858, 1179, 1319 |
| Sau3AI | GATC | 6 cut(s) | 403, 489, 604, 789, 1384, 1522 |
| Sau96I | GGNCC | 9 cut(s) | 173, 293, 559, 1157, 1207, 1284, 1498, 1612, 1613 |
| ScrFI | CCNGG | 5 cut(s) | 171, 327, 600, 798, 1156 |
| SduI | GDGCHC | 3 cut(s) | 317, 696, 1616 |
| SexAI | ACCWGGT | 1 cut(s) | 796 |
| SfaNI | GCATC | 4 cut(s) | 66, 739, 1028, 1444 |
| SfcI | CTRYAG | 1 cut(s) | 861 |
| SinI | GGWCC | 5 cut(s) | 173, 293, 559, 1207, 1498 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 98, 255, 1379, 1440, 1583 |
| SmlI | CTYRAG | 1 cut(s) | 1617 |
| SmoI | CTYRAG | 1 cut(s) | 1617 |
| SphI | GCATGC | 1 cut(s) | 389 |
| SsiI | CCGC | 6 cut(s) | 576, 746, 858, 942, 1179, 1319 |
| SspMI | CTAG | 4 cut(s) | 753, 872, 1182, 1212 |
| StyD4I | CCNGG | 5 cut(s) | 169, 325, 598, 796, 1154 |
| StyI | CCWWGG | 2 cut(s) | 272, 281 |
| TaiI | ACGT | 2 cut(s) | 1174, 1587 |
| TaqI | TCGA | 5 cut(s) | 11, 213, 406, 788, 1521 |
| TaqII | GACCGA | 1 cut(s) | 190 |
| TatI | WGTACW | 2 cut(s) | 415, 1368 |
| TauI | GCSGC | 3 cut(s) | 860, 1181, 1321 |
| TfiI | GAWTC | 4 cut(s) | 277, 319, 623, 1333 |
| Tru1I | TTAA | 7 cut(s) | 51, 222, 588, 1026, 1086, 1296, 1660 |
| Tru9I | TTAA | 7 cut(s) | 51, 222, 588, 1026, 1086, 1296, 1660 |
| TscAI | CASTG | 5 cut(s) | 257, 317, 400, 418, 829 |
| TseFI | GTSAC | 2 cut(s) | 730, 1585 |
| Tsp45I | GTSAC | 2 cut(s) | 730, 1585 |
| TspDTI | ATGAA | 3 cut(s) | 17, 1322, 1582 |
| TspGWI | ACGGA | 3 cut(s) | 306, 676, 1364 |
| TspRI | CASTG | 5 cut(s) | 257, 317, 400, 418, 829 |
| Tth111I | GACNNNGTC | 1 cut(s) | 203 |
| VneI | GTGCAC | 1 cut(s) | 692 |
| VpaK11BI | GGWCC | 5 cut(s) | 173, 293, 559, 1207, 1498 |
| VspI | ATTAAT | 1 cut(s) | 51 |
| XapI | RAATTY | 1 cut(s) | 1276 |
| XceI | RCATGY | 1 cut(s) | 389 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 279 |
| XspI | CTAG | 4 cut(s) | 753, 872, 1182, 1212 |
| Zsp2I | ATGCAT | 2 cut(s) | 1021, 1437 |