Lignin degradation and detoxification of lignin-derived products
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 468 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 38 |
| AccI | GTMKAC | 1 cut(s) | 972 |
| AciI | CCGC | 3 cut(s) | 705, 1049, 1469 |
| AclWI | GGATC | 2 cut(s) | 1283, 1398 |
| AcsI | RAATTY | 5 cut(s) | 678, 727, 1042, 1171, 1246 |
| AcyI | GRCGYC | 1 cut(s) | 1100 |
| AfaI | GTAC | 3 cut(s) | 919, 1091, 1136 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 38, 72, 167, 238, 239, 1109, 1325, 1475 |
| AgsI | TTSAA | 7 cut(s) | 109, 341, 366, 430, 567, 738, 1301 |
| AjiI | CACGTC | 2 cut(s) | 844, 1417 |
| AjnI | CCWGG | 3 cut(s) | 111, 1360, 1447 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 1021, 1053, 1406, 1438 |
| AluBI | AGCT | 5 cut(s) | 118, 433, 816, 998, 1073 |
| AluI | AGCT | 5 cut(s) | 118, 433, 816, 998, 1073 |
| AlwI | GGATC | 2 cut(s) | 1283, 1398 |
| Ama87I | CYCGRG | 2 cut(s) | 590, 946 |
| AoxI | GGCC | 2 cut(s) | 923, 1444 |
| ApaI | GGGCCC | 1 cut(s) | 1448 |
| ApeKI | GCWGC | 1 cut(s) | 650 |
| ApoI | RAATTY | 5 cut(s) | 678, 727, 1042, 1171, 1246 |
| AseI | ATTAAT | 1 cut(s) | 1323 |
| AsiSI | GCGATCGC | 1 cut(s) | 400 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 336 |
| AspS9I | GGNCC | 7 cut(s) | 79, 158, 352, 923, 1264, 1444, 1445 |
| AsuC2I | CCSGG | 3 cut(s) | 393, 591, 592 |
| AsuHPI | GGTGA | 1 cut(s) | 1132 |
| AvaI | CYCGRG | 2 cut(s) | 590, 946 |
| AvaII | GGWCC | 4 cut(s) | 79, 158, 352, 1264 |
| BaeGI | GKGCMC | 2 cut(s) | 937, 1448 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1118, 1151 |
| BanII | GRGCYC | 1 cut(s) | 1448 |
| BbvI | GCAGC | 1 cut(s) | 637 |
| BccI | CCATC | 5 cut(s) | 40, 70, 893, 1200, 1316 |
| BceAI | ACGGC | 3 cut(s) | 463, 467, 1069 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 179, 213 |
| BciT130I | CCWGG | 3 cut(s) | 113, 1362, 1449 |
| BcnI | CCSGG | 3 cut(s) | 393, 591, 592 |
| BfaI | CTAG | 3 cut(s) | 119, 675, 732 |
| BfmI | CTRYAG | 1 cut(s) | 1094 |
| BfuAI | ACCTGC | 1 cut(s) | 468 |
| BisI | GCNGC | 1 cut(s) | 651 |
| BlsI | GCNGC | 1 cut(s) | 652 |
| Bme1390I | CCNGG | 6 cut(s) | 113, 393, 591, 592, 1362, 1449 |
| Bme18I | GGWCC | 4 cut(s) | 79, 158, 352, 1264 |
| BmeT110I | CYCGRG | 2 cut(s) | 590, 946 |
| BmgBI | CACGTC | 2 cut(s) | 844, 1417 |
| BmgT120I | GGNCC | 7 cut(s) | 79, 158, 352, 923, 1264, 1444, 1445 |
| BmiI | GGNNCC | 9 cut(s) | 64, 81, 159, 160, 244, 353, 354, 1266, 1446 |
| BmrFI | CCNGG | 6 cut(s) | 113, 393, 591, 592, 1362, 1449 |
| BmrI | ACTGGG | 1 cut(s) | 930 |
| BmsI | GCATC | 4 cut(s) | 532, 794, 1188, 1210 |
| BmuI | ACTGGG | 1 cut(s) | 930 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1399 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1124, 1156 |
| BpmI | CTGGAG | 1 cut(s) | 1151 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1074 |
| BpuMI | CCSGG | 3 cut(s) | 393, 591, 592 |
| Bsa29I | ATCGAT | 1 cut(s) | 195 |
| BsaBI | GATNNNNATC | 2 cut(s) | 402, 1191 |
| BsaHI | GRCGYC | 1 cut(s) | 1100 |
| BsaJI | CCNNGG | 7 cut(s) | 58, 67, 211, 590, 669, 1207, 1447 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 482, 512, 571, 601 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 38, 72, 167, 238, 239, 1109, 1325, 1475 |
| Bse1I | ACTGG | 3 cut(s) | 801, 925, 1134 |
| Bse3DI | GCAATG | 3 cut(s) | 103, 507, 645 |
| Bse8I | GATNNNNATC | 2 cut(s) | 402, 1191 |
| BseBI | CCWGG | 3 cut(s) | 113, 1362, 1449 |
| BseCI | ATCGAT | 1 cut(s) | 195 |
| BseDI | CCNNGG | 7 cut(s) | 58, 67, 211, 590, 669, 1207, 1447 |
| BseGI | GGATG | 3 cut(s) | 32, 81, 1192 |
| BseJI | GATNNNNATC | 2 cut(s) | 402, 1191 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 38, 72, 167, 238, 239, 1109, 1325, 1475 |
| BseMI | GCAATG | 3 cut(s) | 103, 507, 645 |
| BseMII | CTCAG | 4 cut(s) | 161, 478, 552, 1088 |
| BseNI | ACTGG | 3 cut(s) | 801, 925, 1134 |
| BseRI | GAGGAG | 2 cut(s) | 1197, 1284 |
| BseSI | GKGCMC | 2 cut(s) | 937, 1448 |
| BseXI | GCAGC | 1 cut(s) | 637 |
| BsgI | GTGCAG | 1 cut(s) | 506 |
| Bsh1285I | CGRYCG | 2 cut(s) | 199, 400 |
| BshFI | GGCC | 2 cut(s) | 925, 1446 |
| BshVI | ATCGAT | 1 cut(s) | 195 |
| BsiEI | CGRYCG | 2 cut(s) | 199, 400 |
| BsiHKCI | CYCGRG | 2 cut(s) | 590, 946 |
| BsiSI | CCGG | 2 cut(s) | 393, 591 |
| BslFI | GGGAC | 7 cut(s) | 65, 171, 365, 1126, 1180, 1245, 1301 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 38, 72, 167, 238, 239, 1109, 1325, 1475 |
| BsmFI | GGGAC | 7 cut(s) | 65, 171, 365, 1126, 1180, 1245, 1301 |
| BsmI | GAATGC | 1 cut(s) | 1485 |
| BsnI | GGCC | 2 cut(s) | 925, 1446 |
| BsoBI | CYCGRG | 2 cut(s) | 590, 946 |
| Bsp120I | GGGCCC | 1 cut(s) | 1444 |
| Bsp1286I | GDGCHC | 2 cut(s) | 937, 1448 |
| Bsp143I | GATC | 6 cut(s) | 192, 196, 397, 604, 1288, 1390 |
| Bsp19I | CCATGG | 1 cut(s) | 67 |
| BspACI | CCGC | 3 cut(s) | 705, 1049, 1469 |
| BspANI | GGCC | 2 cut(s) | 925, 1446 |
| BspCNI | CTCAG | 4 cut(s) | 160, 477, 551, 1087 |
| BspDI | ATCGAT | 1 cut(s) | 195 |
| BspLI | GGNNCC | 9 cut(s) | 64, 81, 159, 160, 244, 353, 354, 1266, 1446 |
| BspMI | ACCTGC | 1 cut(s) | 468 |
| BspPI | GGATC | 2 cut(s) | 1283, 1398 |
| BsrDI | GCAATG | 3 cut(s) | 103, 507, 645 |
| BsrI | ACTGG | 3 cut(s) | 801, 925, 1134 |
| BssECI | CCNNGG | 7 cut(s) | 58, 67, 211, 590, 669, 1207, 1447 |
| BssMI | GATC | 6 cut(s) | 192, 196, 397, 604, 1288, 1390 |
| BssNI | GRCGYC | 1 cut(s) | 1100 |
| BssT1I | CCWWGG | 1 cut(s) | 67 |
| Bst2UI | CCWGG | 3 cut(s) | 113, 1362, 1449 |
| Bst4CI | ACNGT | 6 cut(s) | 208, 215, 439, 529, 1147, 1382 |
| Bst6I | CTCTTC | 1 cut(s) | 1302 |
| BstACI | GRCGYC | 1 cut(s) | 1100 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 941 |
| BstC8I | GCNNGC | 2 cut(s) | 173, 996 |
| BstDEI | CTNAG | 4 cut(s) | 147, 464, 538, 1074 |
| BstDSI | CCRYGG | 3 cut(s) | 67, 211, 1207 |
| BstF5I | GGATG | 3 cut(s) | 32, 81, 1192 |
| BstKTI | GATC | 6 cut(s) | 195, 199, 400, 607, 1291, 1393 |
| BstMBI | GATC | 6 cut(s) | 192, 196, 397, 604, 1288, 1390 |
| BstMCI | CGRYCG | 2 cut(s) | 199, 400 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 408, 656, 665, 941 |
| BstNI | CCWGG | 3 cut(s) | 113, 1362, 1449 |
| BstNSI | RCATGY | 1 cut(s) | 175 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1399 |
| BstSCI | CCNGG | 6 cut(s) | 111, 391, 589, 590, 1360, 1447 |
| BstSFI | CTRYAG | 1 cut(s) | 1094 |
| BstSLI | GKGCMC | 2 cut(s) | 937, 1448 |
| BstV1I | GCAGC | 1 cut(s) | 637 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 43, 1016 |
| Bsu15I | ATCGAT | 1 cut(s) | 195 |
| BsuRI | GGCC | 2 cut(s) | 925, 1446 |
| BsuTUI | ATCGAT | 1 cut(s) | 195 |
| BtgI | CCRYGG | 3 cut(s) | 67, 211, 1207 |
| BtgZI | GCGATG | 1 cut(s) | 1294 |
| BtrI | CACGTC | 2 cut(s) | 844, 1417 |
| BtsCI | GGATG | 3 cut(s) | 32, 81, 1192 |
| BtsIMutI | CAGTG | 1 cut(s) | 1378 |
| BveI | ACCTGC | 1 cut(s) | 468 |
| Cac8I | GCNNGC | 2 cut(s) | 173, 996 |
| Cfr13I | GGNCC | 7 cut(s) | 79, 158, 352, 923, 1264, 1444, 1445 |
| Cfr9I | CCCGGG | 1 cut(s) | 590 |
| ClaI | ATCGAT | 1 cut(s) | 195 |
| CseI | GACGC | 1 cut(s) | 1108 |
| Csp6I | GTAC | 3 cut(s) | 918, 1090, 1135 |
| CviAII | CATG | 8 cut(s) | 43, 68, 172, 915, 1010, 1404, 1411, 1488 |
| CviQI | GTAC | 3 cut(s) | 918, 1090, 1135 |
| DdeI | CTNAG | 4 cut(s) | 147, 464, 538, 1074 |
| DpnI | GATC | 6 cut(s) | 194, 198, 399, 606, 1290, 1392 |
| DpnII | GATC | 6 cut(s) | 192, 196, 397, 604, 1288, 1390 |
| Eam1104I | CTCTTC | 1 cut(s) | 1302 |
| EarI | CTCTTC | 1 cut(s) | 1302 |
| EciI | GGCGGA | 1 cut(s) | 1458 |
| Eco130I | CCWWGG | 1 cut(s) | 67 |
| Eco24I | GRGCYC | 1 cut(s) | 1448 |
| Eco47I | GGWCC | 4 cut(s) | 79, 158, 352, 1264 |
| Eco88I | CYCGRG | 2 cut(s) | 590, 946 |
| EcoO109I | RGGNCCY | 4 cut(s) | 158, 352, 1264, 1444 |
| EcoRI | GAATTC | 1 cut(s) | 678 |
| EcoRII | CCWGG | 3 cut(s) | 111, 1360, 1447 |
| EcoT14I | CCWWGG | 1 cut(s) | 67 |
| EcoT22I | ATGCAT | 1 cut(s) | 1203 |
| EcoT38I | GRGCYC | 1 cut(s) | 1448 |
| ErhI | CCWWGG | 1 cut(s) | 67 |
| FaeI | CATG | 8 cut(s) | 46, 71, 175, 918, 1013, 1407, 1414, 1491 |
| FaqI | GGGAC | 7 cut(s) | 65, 171, 365, 1126, 1180, 1245, 1301 |
| FatI | CATG | 8 cut(s) | 42, 67, 171, 914, 1009, 1403, 1410, 1487 |
| FauI | CCCGC | 1 cut(s) | 1056 |
| FauNDI | CATATG | 1 cut(s) | 1398 |
| FblI | GTMKAC | 1 cut(s) | 972 |
| Fnu4HI | GCNGC | 1 cut(s) | 651 |
| FokI | GGATG | 3 cut(s) | 19, 88, 1179 |
| FriOI | GRGCYC | 1 cut(s) | 1448 |
| Fsp4HI | GCNGC | 1 cut(s) | 651 |
| FspBI | CTAG | 3 cut(s) | 119, 675, 732 |
| GluI | GCNGC | 1 cut(s) | 651 |
| GsuI | CTGGAG | 1 cut(s) | 1151 |
| HaeIII | GGCC | 2 cut(s) | 925, 1446 |
| HapII | CCGG | 2 cut(s) | 393, 591 |
| HgaI | GACGC | 1 cut(s) | 1108 |
| Hin1I | GRCGYC | 1 cut(s) | 1100 |
| Hin1II | CATG | 8 cut(s) | 46, 71, 175, 918, 1013, 1407, 1414, 1491 |
| HincII | GTYRAC | 3 cut(s) | 389, 841, 973 |
| HindII | GTYRAC | 3 cut(s) | 389, 841, 973 |
| HindIII | AAGCTT | 2 cut(s) | 814, 996 |
| HinfI | GANTC | 3 cut(s) | 337, 1212, 1344 |
| HpaII | CCGG | 2 cut(s) | 393, 591 |
| HphI | GGTGA | 1 cut(s) | 1132 |
| Hpy166II | GTNNAC | 7 cut(s) | 125, 211, 389, 793, 806, 841, 973 |
| Hpy188I | TCNGA | 7 cut(s) | 75, 150, 467, 541, 716, 1181, 1197 |
| Hpy188III | TCNNGA | 3 cut(s) | 82, 1076, 1388 |
| Hpy8I | GTNNAC | 7 cut(s) | 125, 211, 389, 793, 806, 841, 973 |
| HpyAV | CCTTC | 4 cut(s) | 148, 232, 298, 774 |
| HpyCH4III | ACNGT | 6 cut(s) | 208, 215, 439, 529, 1147, 1382 |
| HpyCH4IV | ACGT | 6 cut(s) | 522, 843, 937, 1224, 1416, 1421 |
| HpyCH4V | TGCA | 4 cut(s) | 487, 994, 1201, 1378 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 408, 656, 665, 941 |
| HpyF3I | CTNAG | 4 cut(s) | 147, 464, 538, 1074 |
| HpySE526I | ACGT | 6 cut(s) | 522, 843, 937, 1224, 1416, 1421 |
| Hsp92I | GRCGYC | 1 cut(s) | 1100 |
| Hsp92II | CATG | 8 cut(s) | 46, 71, 175, 918, 1013, 1407, 1414, 1491 |
| KflI | GGGWCCC | 2 cut(s) | 158, 352 |
| Kzo9I | GATC | 6 cut(s) | 192, 196, 397, 604, 1288, 1390 |
| LmnI | GCTCC | 2 cut(s) | 115, 242 |
| Lsp1109I | GCAGC | 1 cut(s) | 637 |
| LweI | GCATC | 4 cut(s) | 532, 794, 1188, 1210 |
| MaeI | CTAG | 3 cut(s) | 119, 675, 732 |
| MaeII | ACGT | 6 cut(s) | 522, 843, 937, 1224, 1416, 1421 |
| MaeIII | GTNAC | 5 cut(s) | 22, 523, 577, 1417, 1461 |
| MalI | GATC | 6 cut(s) | 194, 198, 399, 606, 1290, 1392 |
| MboI | GATC | 6 cut(s) | 192, 196, 397, 604, 1288, 1390 |
| MboII | GAAGA | 4 cut(s) | 320, 752, 1135, 1289 |
| MhlI | GDGCHC | 2 cut(s) | 937, 1448 |
| MmeI | TCCRAC | 2 cut(s) | 53, 1310 |
| Mph1103I | ATGCAT | 1 cut(s) | 1203 |
| MroXI | GAANNNNTTC | 1 cut(s) | 336 |
| MseI | TTAA | 5 cut(s) | 50, 614, 1062, 1323, 1492 |
| MslI | CAYNNNNRTG | 3 cut(s) | 41, 1014, 1415 |
| MspI | CCGG | 2 cut(s) | 393, 591 |
| MspR9I | CCNGG | 6 cut(s) | 113, 393, 591, 592, 1362, 1449 |
| Mva1269I | GAATGC | 1 cut(s) | 1485 |
| MvaI | CCWGG | 3 cut(s) | 113, 1362, 1449 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 408, 656, 665, 941 |
| NciI | CCSGG | 3 cut(s) | 393, 591, 592 |
| NcoI | CCATGG | 1 cut(s) | 67 |
| NdeI | CATATG | 1 cut(s) | 1398 |
| NdeII | GATC | 6 cut(s) | 192, 196, 397, 604, 1288, 1390 |
| NlaIII | CATG | 8 cut(s) | 46, 71, 175, 918, 1013, 1407, 1414, 1491 |
| NlaIV | GGNNCC | 9 cut(s) | 64, 81, 159, 160, 244, 353, 354, 1266, 1446 |
| NmeAIII | GCCGAG | 1 cut(s) | 650 |
| NmuCI | GTSAC | 2 cut(s) | 523, 1417 |
| NsiI | ATGCAT | 1 cut(s) | 1203 |
| NspI | RCATGY | 1 cut(s) | 175 |
| PaeI | GCATGC | 1 cut(s) | 175 |
| PaeR7I | CTCGAG | 1 cut(s) | 946 |
| PctI | GAATGC | 1 cut(s) | 1485 |
| PdmI | GAANNNNTTC | 1 cut(s) | 336 |
| PfeI | GAWTC | 3 cut(s) | 337, 1212, 1344 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 38 |
| PkrI | GCNGC | 1 cut(s) | 652 |
| Ple19I | CGATCG | 2 cut(s) | 199, 400 |
| PpuMI | RGGWCCY | 3 cut(s) | 158, 352, 1264 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1399 |
| PshBI | ATTAAT | 1 cut(s) | 1323 |
| Psp5II | RGGWCCY | 3 cut(s) | 158, 352, 1264 |
| Psp6I | CCWGG | 3 cut(s) | 111, 1360, 1447 |
| PspGI | CCWGG | 3 cut(s) | 111, 1360, 1447 |
| PspN4I | GGNNCC | 9 cut(s) | 64, 81, 159, 160, 244, 353, 354, 1266, 1446 |
| PspOMI | GGGCCC | 1 cut(s) | 1444 |
| PspPI | GGNCC | 7 cut(s) | 79, 158, 352, 923, 1264, 1444, 1445 |
| PspPPI | RGGWCCY | 3 cut(s) | 158, 352, 1264 |
| PspXI | VCTCGAGB | 1 cut(s) | 946 |
| PvuI | CGATCG | 2 cut(s) | 199, 400 |
| RgaI | GCGATCGC | 1 cut(s) | 400 |
| RsaI | GTAC | 3 cut(s) | 919, 1091, 1136 |
| RsaNI | GTAC | 3 cut(s) | 918, 1090, 1135 |
| RseI | CAYNNNNRTG | 3 cut(s) | 41, 1014, 1415 |
| SalI | GTCGAC | 1 cut(s) | 971 |
| SaqAI | TTAA | 5 cut(s) | 50, 614, 1062, 1323, 1492 |
| SatI | GCNGC | 1 cut(s) | 651 |
| Sau3AI | GATC | 6 cut(s) | 192, 196, 397, 604, 1288, 1390 |
| Sau96I | GGNCC | 7 cut(s) | 79, 158, 352, 923, 1264, 1444, 1445 |
| ScrFI | CCNGG | 6 cut(s) | 113, 393, 591, 592, 1362, 1449 |
| SduI | GDGCHC | 2 cut(s) | 937, 1448 |
| SfaAI | GCGATCGC | 1 cut(s) | 400 |
| SfaNI | GCATC | 4 cut(s) | 532, 794, 1188, 1210 |
| SfcI | CTRYAG | 1 cut(s) | 1094 |
| Sfr274I | CTCGAG | 1 cut(s) | 946 |
| SgfI | GCGATCGC | 1 cut(s) | 400 |
| SinI | GGWCC | 4 cut(s) | 79, 158, 352, 1264 |
| SlaI | CTCGAG | 1 cut(s) | 946 |
| SmaI | CCCGGG | 1 cut(s) | 592 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 41, 1014, 1415 |
| SmlI | CTYRAG | 1 cut(s) | 946 |
| SmoI | CTYRAG | 1 cut(s) | 946 |
| SphI | GCATGC | 1 cut(s) | 175 |
| SsiI | CCGC | 3 cut(s) | 705, 1049, 1469 |
| SspMI | CTAG | 3 cut(s) | 119, 675, 732 |
| StyD4I | CCNGG | 6 cut(s) | 111, 391, 589, 590, 1360, 1447 |
| StyI | CCWWGG | 1 cut(s) | 67 |
| TaaI | ACNGT | 6 cut(s) | 208, 215, 439, 529, 1147, 1382 |
| TaiI | ACGT | 6 cut(s) | 525, 846, 940, 1227, 1419, 1424 |
| TaqI | TCGA | 5 cut(s) | 195, 199, 947, 972, 1393 |
| TatI | WGTACW | 2 cut(s) | 917, 1134 |
| TfiI | GAWTC | 3 cut(s) | 337, 1212, 1344 |
| Tru1I | TTAA | 5 cut(s) | 50, 614, 1062, 1323, 1492 |
| Tru9I | TTAA | 5 cut(s) | 50, 614, 1062, 1323, 1492 |
| TscAI | CASTG | 1 cut(s) | 1385 |
| TseFI | GTSAC | 2 cut(s) | 523, 1417 |
| TseI | GCWGC | 1 cut(s) | 650 |
| Tsp45I | GTSAC | 2 cut(s) | 523, 1417 |
| TspDTI | ATGAA | 3 cut(s) | 345, 903, 1414 |
| TspGWI | ACGGA | 2 cut(s) | 1130, 1224 |
| TspMI | CCCGGG | 1 cut(s) | 590 |
| TspRI | CASTG | 1 cut(s) | 1385 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 38 |
| VpaK11BI | GGWCC | 4 cut(s) | 79, 158, 352, 1264 |
| VspI | ATTAAT | 1 cut(s) | 1323 |
| XapI | RAATTY | 5 cut(s) | 678, 727, 1042, 1171, 1246 |
| XceI | RCATGY | 1 cut(s) | 175 |
| XhoI | CTCGAG | 1 cut(s) | 946 |
| XmaI | CCCGGG | 1 cut(s) | 590 |
| XmiI | GTMKAC | 1 cut(s) | 972 |
| XmnI | GAANNNNTTC | 1 cut(s) | 336 |
| XspI | CTAG | 3 cut(s) | 119, 675, 732 |
| Zsp2I | ATGCAT | 1 cut(s) | 1203 |