Lignin degradation and detoxification of lignin-derived products
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1623 |
| Acc16I | TGCGCA | 1 cut(s) | 270 |
| Acc36I | ACCTGC | 1 cut(s) | 1333 |
| AccB1I | GGYRCC | 1 cut(s) | 374 |
| AccII | CGCG | 3 cut(s) | 929, 1156, 1361 |
| AccIII | TCCGGA | 1 cut(s) | 554 |
| AciI | CCGC | 4 cut(s) | 861, 1164, 1268, 1304 |
| AclWI | GGATC | 2 cut(s) | 414, 1502 |
| AcoI | YGGCCR | 4 cut(s) | 83, 163, 858, 1161 |
| AcuI | CTGAAG | 2 cut(s) | 1298, 1641 |
| AdeI | CACNNNGTG | 1 cut(s) | 315 |
| AfaI | GTAC | 4 cut(s) | 1298, 1310, 1347, 1355 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 172, 384, 1328, 1646 |
| AflIII | ACRYGT | 2 cut(s) | 1110, 1154 |
| AgsI | TTSAA | 6 cut(s) | 326, 551, 640, 954, 1492, 1520 |
| AjnI | CCWGG | 4 cut(s) | 328, 601, 799, 1645 |
| AleI | CACNNNNGTG | 1 cut(s) | 101 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1240, 1272 |
| Alw26I | GTCTC | 3 cut(s) | 626, 670, 1028 |
| AlwI | GGATC | 2 cut(s) | 414, 1502 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1348 |
| Aor13HI | TCCGGA | 1 cut(s) | 554 |
| AoxI | GGCC | 7 cut(s) | 83, 163, 333, 839, 858, 1142, 1161 |
| AspLEI | GCGC | 4 cut(s) | 271, 884, 1139, 1294 |
| AspS9I | GGNCC | 7 cut(s) | 176, 296, 333, 562, 1142, 1483, 1616 |
| AsuC2I | CCSGG | 2 cut(s) | 174, 1141 |
| AsuHPI | GGTGA | 3 cut(s) | 202, 334, 789 |
| AsuNHI | GCTAGC | 1 cut(s) | 755 |
| AvaII | GGWCC | 5 cut(s) | 176, 296, 562, 1483, 1616 |
| BaeGI | GKGCMC | 3 cut(s) | 320, 1124, 1388 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1337, 1370 |
| BalI | TGGCCA | 2 cut(s) | 85, 165 |
| BanI | GGYRCC | 1 cut(s) | 374 |
| BccI | CCATC | 4 cut(s) | 725, 1106, 1419, 1563 |
| BciT130I | CCWGG | 4 cut(s) | 330, 603, 801, 1647 |
| BciVI | GTATCC | 2 cut(s) | 624, 1579 |
| BcnI | CCSGG | 2 cut(s) | 174, 1141 |
| BcoDI | GTCTC | 3 cut(s) | 626, 670, 1028 |
| BfaI | CTAG | 4 cut(s) | 756, 1008, 1167, 1197 |
| BfmI | CTRYAG | 2 cut(s) | 741, 864 |
| BfoI | RGCGCY | 1 cut(s) | 885 |
| BfuAI | ACCTGC | 1 cut(s) | 1333 |
| BfuI | GTATCC | 2 cut(s) | 624, 1579 |
| BisI | GCNGC | 3 cut(s) | 861, 1164, 1304 |
| BlsI | GCNGC | 3 cut(s) | 862, 1165, 1305 |
| BmcAI | AGTACT | 1 cut(s) | 1298 |
| Bme1390I | CCNGG | 6 cut(s) | 174, 330, 603, 801, 1141, 1647 |
| Bme18I | GGWCC | 5 cut(s) | 176, 296, 562, 1483, 1616 |
| BmgT120I | GGNCC | 7 cut(s) | 176, 296, 333, 562, 1142, 1483, 1616 |
| BmiI | GGNNCC | 7 cut(s) | 177, 334, 376, 563, 564, 1485, 1617 |
| BmrFI | CCNGG | 6 cut(s) | 174, 330, 603, 801, 1141, 1647 |
| BmsI | GCATC | 4 cut(s) | 34, 438, 1007, 1429 |
| BmtI | GCTAGC | 1 cut(s) | 759 |
| Bpu10I | CCTNAGC | 1 cut(s) | 843 |
| BpuMI | CCSGG | 2 cut(s) | 174, 1141 |
| BsaAI | YACGTR | 1 cut(s) | 874 |
| BsaBI | GATNNNNATC | 2 cut(s) | 612, 1410 |
| BsaI | GGTCTC | 1 cut(s) | 1028 |
| BsaJI | CCNNGG | 4 cut(s) | 284, 329, 421, 1365 |
| BsaWI | WCCGGW | 2 cut(s) | 554, 689 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1361, 1391 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 172, 384, 1328, 1646 |
| Bse1I | ACTGG | 5 cut(s) | 166, 260, 1024, 1117, 1353 |
| Bse3DI | GCAATG | 1 cut(s) | 1549 |
| Bse8I | GATNNNNATC | 2 cut(s) | 612, 1410 |
| BseAI | TCCGGA | 1 cut(s) | 554 |
| BseBI | CCWGG | 4 cut(s) | 330, 603, 801, 1647 |
| BseDI | CCNNGG | 4 cut(s) | 284, 329, 421, 1365 |
| BseGI | GGATG | 2 cut(s) | 1411, 1411 |
| BseJI | GATNNNNATC | 2 cut(s) | 612, 1410 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 172, 384, 1328, 1646 |
| BseMI | GCAATG | 1 cut(s) | 1549 |
| BseMII | CTCAG | 2 cut(s) | 665, 1039 |
| BseNI | ACTGG | 5 cut(s) | 166, 260, 1024, 1117, 1353 |
| BseRI | GAGGAG | 1 cut(s) | 1503 |
| BseSI | GKGCMC | 3 cut(s) | 320, 1124, 1388 |
| BsgI | GTGCAG | 2 cut(s) | 118, 1361 |
| Bsh1236I | CGCG | 3 cut(s) | 929, 1156, 1361 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1365 |
| BshFI | GGCC | 7 cut(s) | 85, 165, 335, 841, 860, 1144, 1163 |
| BshNI | GGYRCC | 1 cut(s) | 374 |
| BsiEI | CGRYCG | 1 cut(s) | 1365 |
| BsiSI | CCGG | 4 cut(s) | 173, 555, 690, 1140 |
| BslFI | GGGAC | 7 cut(s) | 189, 575, 760, 1165, 1345, 1520, 1629 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 172, 384, 1328, 1646 |
| BsmAI | GTCTC | 3 cut(s) | 626, 670, 1028 |
| BsmBI | CGTCTC | 1 cut(s) | 626 |
| BsmFI | GGGAC | 7 cut(s) | 189, 575, 760, 1165, 1345, 1520, 1629 |
| BsnI | GGCC | 7 cut(s) | 85, 165, 335, 841, 860, 1144, 1163 |
| Bso31I | GGTCTC | 1 cut(s) | 1028 |
| Bsp1286I | GDGCHC | 3 cut(s) | 320, 1124, 1388 |
| Bsp13I | TCCGGA | 1 cut(s) | 554 |
| Bsp1407I | TGTACA | 1 cut(s) | 1353 |
| Bsp143I | GATC | 5 cut(s) | 351, 406, 607, 970, 1507 |
| Bsp19I | CCATGG | 2 cut(s) | 284, 421 |
| BspACI | CCGC | 4 cut(s) | 861, 1164, 1268, 1304 |
| BspANI | GGCC | 7 cut(s) | 85, 165, 335, 841, 860, 1144, 1163 |
| BspCNI | CTCAG | 2 cut(s) | 666, 1038 |
| BspEI | TCCGGA | 1 cut(s) | 554 |
| BspFNI | CGCG | 3 cut(s) | 929, 1156, 1361 |
| BspLI | GGNNCC | 7 cut(s) | 177, 334, 376, 563, 564, 1485, 1617 |
| BspMI | ACCTGC | 1 cut(s) | 1333 |
| BspOI | GCTAGC | 1 cut(s) | 759 |
| BspPI | GGATC | 2 cut(s) | 414, 1502 |
| BspT107I | GGYRCC | 1 cut(s) | 374 |
| BspTNI | GGTCTC | 1 cut(s) | 1028 |
| BsrDI | GCAATG | 1 cut(s) | 1549 |
| BsrGI | TGTACA | 1 cut(s) | 1353 |
| BsrI | ACTGG | 5 cut(s) | 166, 260, 1024, 1117, 1353 |
| BssECI | CCNNGG | 4 cut(s) | 284, 329, 421, 1365 |
| BssMI | GATC | 5 cut(s) | 351, 406, 607, 970, 1507 |
| BssT1I | CCWWGG | 2 cut(s) | 284, 421 |
| Bst2UI | CCWGG | 4 cut(s) | 330, 603, 801, 1647 |
| Bst4CI | ACNGT | 8 cut(s) | 157, 214, 315, 398, 418, 1278, 1366, 1436 |
| Bst6I | CTCTTC | 1 cut(s) | 1521 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1300 |
| BstAUI | TGTACA | 1 cut(s) | 1353 |
| BstBAI | YACGTR | 1 cut(s) | 874 |
| BstC8I | GCNNGC | 3 cut(s) | 87, 390, 757 |
| BstDEI | CTNAG | 3 cut(s) | 674, 843, 1025 |
| BstDSI | CCRYGG | 3 cut(s) | 284, 421, 1365 |
| BstF5I | GGATG | 2 cut(s) | 1411, 1411 |
| BstFNI | CGCG | 3 cut(s) | 929, 1156, 1361 |
| BstH2I | RGCGCY | 1 cut(s) | 885 |
| BstHHI | GCGC | 4 cut(s) | 271, 884, 1139, 1294 |
| BstKTI | GATC | 5 cut(s) | 354, 409, 610, 973, 1510 |
| BstMAI | GTCTC | 3 cut(s) | 626, 670, 1028 |
| BstMBI | GATC | 5 cut(s) | 351, 406, 607, 970, 1507 |
| BstMCI | CGRYCG | 1 cut(s) | 1365 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 86, 866, 1300 |
| BstNI | CCWGG | 4 cut(s) | 330, 603, 801, 1647 |
| BstNSI | RCATGY | 1 cut(s) | 392 |
| BstSCI | CCNGG | 6 cut(s) | 172, 328, 601, 799, 1139, 1645 |
| BstSFI | CTRYAG | 2 cut(s) | 741, 864 |
| BstSLI | GKGCMC | 3 cut(s) | 320, 1124, 1388 |
| BstSNI | TACGTA | 1 cut(s) | 874 |
| BstUI | CGCG | 3 cut(s) | 929, 1156, 1361 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 260 |
| BsuI | GTATCC | 2 cut(s) | 624, 1579 |
| BsuRI | GGCC | 7 cut(s) | 85, 165, 335, 841, 860, 1144, 1163 |
| BtgI | CCRYGG | 3 cut(s) | 284, 421, 1365 |
| BtgZI | GCGATG | 1 cut(s) | 703 |
| BtsCI | GGATG | 2 cut(s) | 1411, 1411 |
| BtsI | GCAGTG | 1 cut(s) | 70 |
| BtsIMutI | CAGTG | 6 cut(s) | 70, 253, 320, 403, 1017, 1124 |
| BveI | ACCTGC | 1 cut(s) | 1333 |
| Cac8I | GCNNGC | 3 cut(s) | 87, 390, 757 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1348 |
| CfoI | GCGC | 4 cut(s) | 271, 884, 1139, 1294 |
| Cfr13I | GGNCC | 7 cut(s) | 176, 296, 333, 562, 1142, 1483, 1616 |
| CseI | GACGC | 4 cut(s) | 587, 761, 1145, 1162 |
| CsiI | ACCWGGT | 2 cut(s) | 799, 1645 |
| Csp6I | GTAC | 4 cut(s) | 1297, 1309, 1346, 1354 |
| CviQI | GTAC | 4 cut(s) | 1297, 1309, 1346, 1354 |
| DdeI | CTNAG | 3 cut(s) | 674, 843, 1025 |
| DpnI | GATC | 5 cut(s) | 353, 408, 609, 972, 1509 |
| DpnII | GATC | 5 cut(s) | 351, 406, 607, 970, 1507 |
| DraIII | CACNNNGTG | 1 cut(s) | 315 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1623 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1623 |
| EaeI | YGGCCR | 4 cut(s) | 83, 163, 858, 1161 |
| Eam1104I | CTCTTC | 1 cut(s) | 1521 |
| EarI | CTCTTC | 1 cut(s) | 1521 |
| Eco105I | TACGTA | 1 cut(s) | 874 |
| Eco130I | CCWWGG | 2 cut(s) | 284, 421 |
| Eco147I | AGGCCT | 1 cut(s) | 841 |
| Eco31I | GGTCTC | 1 cut(s) | 1028 |
| Eco32I | GATATC | 1 cut(s) | 281 |
| Eco47I | GGWCC | 5 cut(s) | 176, 296, 562, 1483, 1616 |
| Eco57I | CTGAAG | 2 cut(s) | 1298, 1641 |
| EcoO109I | RGGNCCY | 3 cut(s) | 562, 1483, 1616 |
| EcoRII | CCWGG | 4 cut(s) | 328, 601, 799, 1645 |
| EcoRV | GATATC | 1 cut(s) | 281 |
| EcoT14I | CCWWGG | 2 cut(s) | 284, 421 |
| EcoT22I | ATGCAT | 3 cut(s) | 1000, 1422, 1551 |
| ErhI | CCWWGG | 2 cut(s) | 284, 421 |
| Esp3I | CGTCTC | 1 cut(s) | 626 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 134, 166, 363, 395 |
| FaqI | GGGAC | 7 cut(s) | 189, 575, 760, 1165, 1345, 1520, 1629 |
| FauI | CCCGC | 1 cut(s) | 1275 |
| FauNDI | CATATG | 1 cut(s) | 1569 |
| Fnu4HI | GCNGC | 3 cut(s) | 861, 1164, 1304 |
| FokI | GGATG | 2 cut(s) | 1398, 1418 |
| Fsp4HI | GCNGC | 3 cut(s) | 861, 1164, 1304 |
| FspBI | CTAG | 4 cut(s) | 756, 1008, 1167, 1197 |
| FspI | TGCGCA | 1 cut(s) | 270 |
| GlaI | GCGC | 4 cut(s) | 270, 883, 1138, 1293 |
| GluI | GCNGC | 3 cut(s) | 861, 1164, 1304 |
| HaeII | RGCGCY | 1 cut(s) | 885 |
| HaeIII | GGCC | 7 cut(s) | 85, 165, 335, 841, 860, 1144, 1163 |
| HapII | CCGG | 4 cut(s) | 173, 555, 690, 1140 |
| HgaI | GACGC | 4 cut(s) | 587, 761, 1145, 1162 |
| HhaI | GCGC | 4 cut(s) | 271, 884, 1139, 1294 |
| Hin6I | GCGC | 4 cut(s) | 269, 882, 1137, 1292 |
| HinP1I | GCGC | 4 cut(s) | 269, 882, 1137, 1292 |
| HincII | GTYRAC | 4 cut(s) | 599, 898, 1108, 1234 |
| HindII | GTYRAC | 4 cut(s) | 599, 898, 1108, 1234 |
| HindIII | AAGCTT | 3 cut(s) | 78, 530, 1215 |
| HinfI | GANTC | 4 cut(s) | 129, 703, 931, 1081 |
| HpaI | GTTAAC | 1 cut(s) | 898 |
| HpaII | CCGG | 4 cut(s) | 173, 555, 690, 1140 |
| HphI | GGTGA | 3 cut(s) | 202, 334, 789 |
| Hpy166II | GTNNAC | 9 cut(s) | 193, 342, 599, 898, 1108, 1174, 1192, 1234, 1354 |
| Hpy188I | TCNGA | 5 cut(s) | 292, 577, 631, 837, 1317 |
| Hpy188III | TCNNGA | 9 cut(s) | 12, 151, 361, 555, 640, 968, 1027, 1259, 1559 |
| Hpy8I | GTNNAC | 9 cut(s) | 193, 342, 599, 898, 1108, 1174, 1192, 1234, 1354 |
| Hpy99I | CGWCG | 2 cut(s) | 218, 581 |
| HpyAV | CCTTC | 6 cut(s) | 358, 365, 508, 957, 1322, 1616 |
| HpyCH4III | ACNGT | 8 cut(s) | 157, 214, 315, 398, 418, 1278, 1366, 1436 |
| HpyCH4IV | ACGT | 4 cut(s) | 635, 873, 1062, 1112 |
| HpyCH4V | TGCA | 9 cut(s) | 25, 31, 135, 482, 697, 998, 1342, 1420, 1549 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 86, 866, 1300 |
| HpyF3I | CTNAG | 3 cut(s) | 674, 843, 1025 |
| HpySE526I | ACGT | 4 cut(s) | 635, 873, 1062, 1112 |
| HspAI | GCGC | 4 cut(s) | 269, 882, 1137, 1292 |
| KflI | GGGWCCC | 1 cut(s) | 562 |
| Kpn2I | TCCGGA | 1 cut(s) | 554 |
| KspAI | GTTAAC | 1 cut(s) | 898 |
| Kzo9I | GATC | 5 cut(s) | 351, 406, 607, 970, 1507 |
| LmnI | GCTCC | 2 cut(s) | 1029, 1369 |
| LweI | GCATC | 4 cut(s) | 34, 438, 1007, 1429 |
| MabI | ACCWGGT | 2 cut(s) | 799, 1645 |
| MaeI | CTAG | 4 cut(s) | 756, 1008, 1167, 1197 |
| MaeII | ACGT | 4 cut(s) | 635, 873, 1062, 1112 |
| MaeIII | GTNAC | 6 cut(s) | 92, 239, 733, 787, 938, 1430 |
| MalI | GATC | 5 cut(s) | 353, 408, 609, 972, 1509 |
| MboI | GATC | 5 cut(s) | 351, 406, 607, 970, 1507 |
| MboII | GAAGA | 4 cut(s) | 942, 965, 1415, 1508 |
| MhlI | GDGCHC | 3 cut(s) | 320, 1124, 1388 |
| MlsI | TGGCCA | 2 cut(s) | 85, 165 |
| MluCI | AATT | 8 cut(s) | 15, 321, 486, 681, 1002, 1074, 1129, 1390 |
| MluI | ACGCGT | 1 cut(s) | 1154 |
| MluNI | TGGCCA | 2 cut(s) | 85, 165 |
| MlyI | GAGTC | 2 cut(s) | 123, 1075 |
| MmeI | TCCRAC | 2 cut(s) | 600, 1429 |
| Mox20I | TGGCCA | 2 cut(s) | 85, 165 |
| Mph1103I | ATGCAT | 3 cut(s) | 1000, 1422, 1551 |
| MroI | TCCGGA | 1 cut(s) | 554 |
| MscI | TGGCCA | 2 cut(s) | 85, 165 |
| MseI | TTAA | 5 cut(s) | 57, 225, 684, 897, 1281 |
| MslI | CAYNNNNRTG | 3 cut(s) | 101, 258, 1425 |
| Msp20I | TGGCCA | 2 cut(s) | 85, 165 |
| MspA1I | CMGCKG | 2 cut(s) | 740, 1306 |
| MspI | CCGG | 4 cut(s) | 173, 555, 690, 1140 |
| MspR9I | CCNGG | 6 cut(s) | 174, 330, 603, 801, 1141, 1647 |
| MvaI | CCWGG | 4 cut(s) | 330, 603, 801, 1647 |
| MvnI | CGCG | 3 cut(s) | 929, 1156, 1361 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 86, 866, 1300 |
| NciI | CCSGG | 2 cut(s) | 174, 1141 |
| NcoI | CCATGG | 2 cut(s) | 284, 421 |
| NdeI | CATATG | 1 cut(s) | 1569 |
| NdeII | GATC | 5 cut(s) | 351, 406, 607, 970, 1507 |
| NheI | GCTAGC | 1 cut(s) | 755 |
| NlaIV | GGNNCC | 7 cut(s) | 177, 334, 376, 563, 564, 1485, 1617 |
| NmuCI | GTSAC | 2 cut(s) | 733, 787 |
| NsbI | TGCGCA | 1 cut(s) | 270 |
| NsiI | ATGCAT | 3 cut(s) | 1000, 1422, 1551 |
| NspI | RCATGY | 1 cut(s) | 392 |
| OliI | CACNNNNGTG | 1 cut(s) | 101 |
| PaeI | GCATGC | 1 cut(s) | 392 |
| PceI | AGGCCT | 1 cut(s) | 841 |
| PfeI | GAWTC | 2 cut(s) | 703, 931 |
| PfoI | TCCNGGA | 1 cut(s) | 172 |
| PkrI | GCNGC | 3 cut(s) | 862, 1165, 1305 |
| PleI | GAGTC | 2 cut(s) | 123, 1075 |
| PpsI | GAGTC | 2 cut(s) | 123, 1075 |
| Ppu21I | YACGTR | 1 cut(s) | 874 |
| PpuMI | RGGWCCY | 3 cut(s) | 562, 1483, 1616 |
| Psp5II | RGGWCCY | 3 cut(s) | 562, 1483, 1616 |
| Psp6I | CCWGG | 4 cut(s) | 328, 601, 799, 1645 |
| PspGI | CCWGG | 4 cut(s) | 328, 601, 799, 1645 |
| PspN4I | GGNNCC | 7 cut(s) | 177, 334, 376, 563, 564, 1485, 1617 |
| PspPI | GGNCC | 7 cut(s) | 176, 296, 333, 562, 1142, 1483, 1616 |
| PspPPI | RGGWCCY | 3 cut(s) | 562, 1483, 1616 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 443, 475 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1348 |
| PvuII | CAGCTG | 1 cut(s) | 740 |
| RsaI | GTAC | 4 cut(s) | 1298, 1310, 1347, 1355 |
| RsaNI | GTAC | 4 cut(s) | 1297, 1309, 1346, 1354 |
| RseI | CAYNNNNRTG | 3 cut(s) | 101, 258, 1425 |
| SaqAI | TTAA | 5 cut(s) | 57, 225, 684, 897, 1281 |
| SatI | GCNGC | 3 cut(s) | 861, 1164, 1304 |
| Sau3AI | GATC | 5 cut(s) | 351, 406, 607, 970, 1507 |
| Sau96I | GGNCC | 7 cut(s) | 176, 296, 333, 562, 1142, 1483, 1616 |
| ScaI | AGTACT | 1 cut(s) | 1298 |
| SchI | GAGTC | 2 cut(s) | 123, 1075 |
| ScrFI | CCNGG | 6 cut(s) | 174, 330, 603, 801, 1141, 1647 |
| SduI | GDGCHC | 3 cut(s) | 320, 1124, 1388 |
| SexAI | ACCWGGT | 2 cut(s) | 799, 1645 |
| SfaNI | GCATC | 4 cut(s) | 34, 438, 1007, 1429 |
| SfcI | CTRYAG | 2 cut(s) | 741, 864 |
| SinI | GGWCC | 5 cut(s) | 176, 296, 562, 1483, 1616 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 101, 258, 1425 |
| SnaBI | TACGTA | 1 cut(s) | 874 |
| SphI | GCATGC | 1 cut(s) | 392 |
| Sse9I | AATT | 8 cut(s) | 15, 321, 486, 681, 1002, 1074, 1129, 1390 |
| SseBI | AGGCCT | 1 cut(s) | 841 |
| SsiI | CCGC | 4 cut(s) | 861, 1164, 1268, 1304 |
| SspMI | CTAG | 4 cut(s) | 756, 1008, 1167, 1197 |
| StuI | AGGCCT | 1 cut(s) | 841 |
| StyD4I | CCNGG | 6 cut(s) | 172, 328, 601, 799, 1139, 1645 |
| StyI | CCWWGG | 2 cut(s) | 284, 421 |
| TaaI | ACNGT | 8 cut(s) | 157, 214, 315, 398, 418, 1278, 1366, 1436 |
| TaiI | ACGT | 4 cut(s) | 638, 876, 1065, 1115 |
| TaqI | TCGA | 2 cut(s) | 216, 362 |
| TasI | AATT | 8 cut(s) | 15, 321, 486, 681, 1002, 1074, 1129, 1390 |
| TatI | WGTACW | 2 cut(s) | 1296, 1353 |
| TauI | GCSGC | 3 cut(s) | 863, 1166, 1306 |
| TfiI | GAWTC | 2 cut(s) | 703, 931 |
| Tru1I | TTAA | 5 cut(s) | 57, 225, 684, 897, 1281 |
| Tru9I | TTAA | 5 cut(s) | 57, 225, 684, 897, 1281 |
| TscAI | CASTG | 6 cut(s) | 77, 260, 320, 403, 1024, 1124 |
| TseFI | GTSAC | 2 cut(s) | 733, 787 |
| Tsp45I | GTSAC | 2 cut(s) | 733, 787 |
| TspDTI | ATGAA | 5 cut(s) | 17, 815, 1122, 1415, 1585 |
| TspGWI | ACGGA | 2 cut(s) | 309, 1093 |
| TspRI | CASTG | 6 cut(s) | 77, 260, 320, 403, 1024, 1124 |
| VpaK11BI | GGWCC | 5 cut(s) | 176, 296, 562, 1483, 1616 |
| XceI | RCATGY | 1 cut(s) | 392 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 282 |
| XspI | CTAG | 4 cut(s) | 756, 1008, 1167, 1197 |
| ZrmI | AGTACT | 1 cut(s) | 1298 |
| Zsp2I | ATGCAT | 3 cut(s) | 1000, 1422, 1551 |