Lignin degradation and detoxification of lignin-derived products
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 783 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 360 |
| AccI | GTMKAC | 1 cut(s) | 158 |
| AccII | CGCG | 1 cut(s) | 1789 |
| AciI | CCGC | 7 cut(s) | 660, 935, 1163, 1260, 1667, 1784, 1787 |
| AclWI | GGATC | 2 cut(s) | 519, 1598 |
| AcoI | YGGCCR | 3 cut(s) | 268, 631, 907 |
| AcsI | RAATTY | 5 cut(s) | 590, 942, 1042, 1357, 1561 |
| AcuI | CTGAAG | 1 cut(s) | 1598 |
| AfaI | GTAC | 4 cut(s) | 641, 1406, 1410, 1451 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 360, 553, 554, 990, 1640, 1790 |
| AgsI | TTSAA | 5 cut(s) | 133, 656, 681, 1531, 1663 |
| AhlI | ACTAGT | 1 cut(s) | 14 |
| AjiI | CACGTC | 1 cut(s) | 1159 |
| AjnI | CCWGG | 4 cut(s) | 277, 433, 706, 1675 |
| AleI | CACNNNNGTG | 1 cut(s) | 206 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1336, 1368 |
| AluBI | AGCT | 8 cut(s) | 129, 185, 748, 864, 1131, 1281, 1313, 1388 |
| AluI | AGCT | 8 cut(s) | 129, 185, 748, 864, 1131, 1281, 1313, 1388 |
| Alw21I | GWGCWC | 1 cut(s) | 1622 |
| AlwI | GGATC | 2 cut(s) | 519, 1598 |
| AoxI | GGCC | 6 cut(s) | 268, 631, 907, 1238, 1723, 1759 |
| ApeKI | GCWGC | 1 cut(s) | 965 |
| ApoI | RAATTY | 5 cut(s) | 590, 942, 1042, 1357, 1561 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 651 |
| AspA2I | CCTAGG | 1 cut(s) | 1762 |
| AspLEI | GCGC | 1 cut(s) | 1771 |
| AspS9I | GGNCC | 6 cut(s) | 401, 937, 1238, 1579, 1723, 1759 |
| AsuC2I | CCSGG | 3 cut(s) | 906, 985, 1415 |
| AsuHPI | GGTGA | 1 cut(s) | 1447 |
| AsuNHI | GCTAGC | 1 cut(s) | 860 |
| AvaII | GGWCC | 3 cut(s) | 401, 937, 1579 |
| AvrII | CCTAGG | 1 cut(s) | 1762 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 300, 333, 1433, 1466 |
| Bbv12I | GWGCWC | 1 cut(s) | 1622 |
| BbvI | GCAGC | 1 cut(s) | 952 |
| BccI | CCATC | 7 cut(s) | 51, 362, 1490, 1515, 1542, 1631, 1745 |
| BceAI | ACGGC | 2 cut(s) | 283, 1384 |
| BciT130I | CCWGG | 4 cut(s) | 279, 435, 708, 1677 |
| BcnI | CCSGG | 3 cut(s) | 906, 985, 1415 |
| BcuI | ACTAGT | 1 cut(s) | 14 |
| BfaI | CTAG | 4 cut(s) | 15, 861, 1727, 1763 |
| BfmI | CTRYAG | 3 cut(s) | 534, 795, 976 |
| BfuAI | ACCTGC | 1 cut(s) | 783 |
| BisI | GCNGC | 4 cut(s) | 966, 1163, 1260, 1787 |
| BlnI | CCTAGG | 1 cut(s) | 1762 |
| BlpI | GCTNAGC | 1 cut(s) | 1389 |
| BlsI | GCNGC | 4 cut(s) | 967, 1164, 1261, 1788 |
| Bme1390I | CCNGG | 7 cut(s) | 279, 435, 708, 906, 985, 1415, 1677 |
| Bme18I | GGWCC | 3 cut(s) | 401, 937, 1579 |
| BmgBI | CACGTC | 1 cut(s) | 1159 |
| BmgT120I | GGNCC | 6 cut(s) | 401, 937, 1238, 1579, 1723, 1759 |
| BmiI | GGNNCC | 5 cut(s) | 439, 559, 996, 1581, 1724 |
| BmrFI | CCNGG | 7 cut(s) | 279, 435, 708, 906, 985, 1415, 1677 |
| BmsI | GCATC | 5 cut(s) | 77, 847, 1109, 1503, 1525 |
| BmtI | GCTAGC | 1 cut(s) | 864 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1714 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1439, 1471 |
| BpmI | CTGGAG | 2 cut(s) | 324, 1466 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1389 |
| BpuMI | CCSGG | 3 cut(s) | 906, 985, 1415 |
| BsaAI | YACGTR | 1 cut(s) | 1408 |
| BsaBI | GATNNNNATC | 2 cut(s) | 717, 1506 |
| BsaJI | CCNNGG | 7 cut(s) | 380, 389, 526, 984, 1036, 1522, 1762 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 886, 916, 1457, 1487 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 360, 553, 554, 990, 1640, 1790 |
| Bse1I | ACTGG | 5 cut(s) | 166, 365, 1116, 1263, 1449 |
| Bse3DI | GCAATG | 4 cut(s) | 175, 425, 822, 960 |
| Bse8I | GATNNNNATC | 2 cut(s) | 717, 1506 |
| BseBI | CCWGG | 4 cut(s) | 279, 435, 708, 1677 |
| BseDI | CCNNGG | 7 cut(s) | 380, 389, 526, 984, 1036, 1522, 1762 |
| BseGI | GGATG | 5 cut(s) | 43, 354, 1501, 1507, 1553 |
| BseJI | GATNNNNATC | 2 cut(s) | 717, 1506 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 360, 553, 554, 990, 1640, 1790 |
| BseMI | GCAATG | 4 cut(s) | 175, 425, 822, 960 |
| BseMII | CTCAG | 4 cut(s) | 483, 793, 867, 1403 |
| BseNI | ACTGG | 5 cut(s) | 166, 365, 1116, 1263, 1449 |
| BseRI | GAGGAG | 2 cut(s) | 183, 1599 |
| BseXI | GCAGC | 1 cut(s) | 952 |
| BseYI | CCCAGC | 2 cut(s) | 989, 1235 |
| Bsh1236I | CGCG | 1 cut(s) | 1789 |
| Bsh1285I | CGRYCG | 2 cut(s) | 107, 1708 |
| BshFI | GGCC | 6 cut(s) | 270, 633, 909, 1240, 1725, 1761 |
| BsiEI | CGRYCG | 2 cut(s) | 107, 1708 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1622 |
| BsiSI | CCGG | 4 cut(s) | 762, 906, 984, 1415 |
| BslFI | GGGAC | 5 cut(s) | 493, 680, 1261, 1495, 1616 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 360, 553, 554, 990, 1640, 1790 |
| BsmFI | GGGAC | 5 cut(s) | 493, 680, 1261, 1495, 1616 |
| BsmI | GAATGC | 1 cut(s) | 1800 |
| BsnI | GGCC | 6 cut(s) | 270, 633, 909, 1240, 1725, 1761 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1622 |
| Bsp1407I | TGTACA | 1 cut(s) | 639 |
| Bsp143I | GATC | 5 cut(s) | 104, 511, 712, 1603, 1705 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1389 |
| Bsp19I | CCATGG | 2 cut(s) | 389, 1522 |
| BspACI | CCGC | 7 cut(s) | 660, 935, 1163, 1260, 1667, 1784, 1787 |
| BspANI | GGCC | 6 cut(s) | 270, 633, 909, 1240, 1725, 1761 |
| BspCNI | CTCAG | 4 cut(s) | 482, 792, 866, 1402 |
| BspFNI | CGCG | 1 cut(s) | 1789 |
| BspHI | TCATGA | 1 cut(s) | 1731 |
| BspLI | GGNNCC | 5 cut(s) | 439, 559, 996, 1581, 1724 |
| BspMI | ACCTGC | 1 cut(s) | 783 |
| BspOI | GCTAGC | 1 cut(s) | 864 |
| BspPI | GGATC | 2 cut(s) | 519, 1598 |
| BsrDI | GCAATG | 4 cut(s) | 175, 425, 822, 960 |
| BsrGI | TGTACA | 1 cut(s) | 639 |
| BsrI | ACTGG | 5 cut(s) | 166, 365, 1116, 1263, 1449 |
| BssECI | CCNNGG | 7 cut(s) | 380, 389, 526, 984, 1036, 1522, 1762 |
| BssMI | GATC | 5 cut(s) | 104, 511, 712, 1603, 1705 |
| BssT1I | CCWWGG | 4 cut(s) | 389, 1036, 1522, 1762 |
| Bst2UI | CCWGG | 4 cut(s) | 279, 435, 708, 1677 |
| Bst4CI | ACNGT | 9 cut(s) | 237, 262, 401, 523, 530, 639, 644, 844, 1697 |
| Bst6I | CTCTTC | 1 cut(s) | 1617 |
| BstAUI | TGTACA | 1 cut(s) | 639 |
| BstBAI | YACGTR | 1 cut(s) | 1408 |
| BstC8I | GCNNGC | 4 cut(s) | 495, 862, 1311, 1425 |
| BstDEI | CTNAG | 4 cut(s) | 469, 779, 853, 1389 |
| BstDSI | CCRYGG | 3 cut(s) | 389, 526, 1522 |
| BstF5I | GGATG | 5 cut(s) | 43, 354, 1501, 1507, 1553 |
| BstFNI | CGCG | 1 cut(s) | 1789 |
| BstHHI | GCGC | 1 cut(s) | 1771 |
| BstKTI | GATC | 5 cut(s) | 107, 514, 715, 1606, 1708 |
| BstMBI | GATC | 5 cut(s) | 104, 511, 712, 1603, 1705 |
| BstMCI | CGRYCG | 2 cut(s) | 107, 1708 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 971, 980, 1424, 1795 |
| BstNI | CCWGG | 4 cut(s) | 279, 435, 708, 1677 |
| BstNSI | RCATGY | 2 cut(s) | 497, 1210 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1714 |
| BstSCI | CCNGG | 7 cut(s) | 277, 433, 706, 904, 983, 1413, 1675 |
| BstSFI | CTRYAG | 3 cut(s) | 534, 795, 976 |
| BstSNI | TACGTA | 1 cut(s) | 1408 |
| BstUI | CGCG | 1 cut(s) | 1789 |
| BstV1I | GCAGC | 1 cut(s) | 952 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 278, 365, 1331 |
| BsuRI | GGCC | 6 cut(s) | 270, 633, 909, 1240, 1725, 1761 |
| BtgI | CCRYGG | 3 cut(s) | 389, 526, 1522 |
| BtgZI | GCGATG | 1 cut(s) | 1609 |
| BtrI | CACGTC | 1 cut(s) | 1159 |
| BtsCI | GGATG | 5 cut(s) | 43, 354, 1501, 1507, 1553 |
| BtsI | GCAGTG | 1 cut(s) | 1017 |
| BtsIMutI | CAGTG | 5 cut(s) | 358, 649, 1017, 1270, 1693 |
| BveI | ACCTGC | 1 cut(s) | 783 |
| Cac8I | GCNNGC | 4 cut(s) | 495, 862, 1311, 1425 |
| CciI | TCATGA | 1 cut(s) | 1731 |
| CfoI | GCGC | 1 cut(s) | 1771 |
| Cfr13I | GGNCC | 6 cut(s) | 401, 937, 1238, 1579, 1723, 1759 |
| CseI | GACGC | 1 cut(s) | 677 |
| Csp6I | GTAC | 4 cut(s) | 640, 1405, 1409, 1450 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1473, 1508 |
| CviQI | GTAC | 4 cut(s) | 640, 1405, 1409, 1450 |
| DdeI | CTNAG | 4 cut(s) | 469, 779, 853, 1389 |
| DpnI | GATC | 5 cut(s) | 106, 513, 714, 1605, 1707 |
| DpnII | GATC | 5 cut(s) | 104, 511, 712, 1603, 1705 |
| DraI | TTTAAA | 1 cut(s) | 9 |
| EaeI | YGGCCR | 3 cut(s) | 268, 631, 907 |
| Eam1104I | CTCTTC | 1 cut(s) | 1617 |
| EarI | CTCTTC | 1 cut(s) | 1617 |
| EciI | GGCGGA | 2 cut(s) | 1682, 1773 |
| Eco105I | TACGTA | 1 cut(s) | 1408 |
| Eco130I | CCWWGG | 4 cut(s) | 389, 1036, 1522, 1762 |
| Eco32I | GATATC | 2 cut(s) | 325, 628 |
| Eco47I | GGWCC | 3 cut(s) | 401, 937, 1579 |
| Eco57I | CTGAAG | 1 cut(s) | 1598 |
| EcoO109I | RGGNCCY | 3 cut(s) | 1579, 1723, 1759 |
| EcoRII | CCWGG | 4 cut(s) | 277, 433, 706, 1675 |
| EcoRV | GATATC | 2 cut(s) | 325, 628 |
| EcoT14I | CCWWGG | 4 cut(s) | 389, 1036, 1522, 1762 |
| EcoT22I | ATGCAT | 1 cut(s) | 1518 |
| ErhI | CCWWGG | 4 cut(s) | 389, 1036, 1522, 1762 |
| FaqI | GGGAC | 5 cut(s) | 493, 680, 1261, 1495, 1616 |
| FauNDI | CATATG | 1 cut(s) | 1713 |
| FblI | GTMKAC | 1 cut(s) | 158 |
| Fnu4HI | GCNGC | 4 cut(s) | 966, 1163, 1260, 1787 |
| FokI | GGATG | 5 cut(s) | 30, 341, 1494, 1508, 1560 |
| Fsp4HI | GCNGC | 4 cut(s) | 966, 1163, 1260, 1787 |
| FspBI | CTAG | 4 cut(s) | 15, 861, 1727, 1763 |
| GlaI | GCGC | 1 cut(s) | 1770 |
| GluI | GCNGC | 4 cut(s) | 966, 1163, 1260, 1787 |
| GsaI | CCCAGC | 2 cut(s) | 993, 1239 |
| GsuI | CTGGAG | 2 cut(s) | 324, 1466 |
| HaeIII | GGCC | 6 cut(s) | 270, 633, 909, 1240, 1725, 1761 |
| HapII | CCGG | 4 cut(s) | 762, 906, 984, 1415 |
| HgaI | GACGC | 1 cut(s) | 677 |
| HhaI | GCGC | 1 cut(s) | 1771 |
| Hin6I | GCGC | 1 cut(s) | 1769 |
| HinP1I | GCGC | 1 cut(s) | 1769 |
| HincII | GTYRAC | 5 cut(s) | 704, 1156, 1204, 1270, 1288 |
| HindII | GTYRAC | 5 cut(s) | 704, 1156, 1204, 1270, 1288 |
| HindIII | AAGCTT | 3 cut(s) | 183, 1129, 1311 |
| HinfI | GANTC | 6 cut(s) | 385, 481, 652, 1249, 1527, 1659 |
| HpaII | CCGG | 4 cut(s) | 762, 906, 984, 1415 |
| HphI | GGTGA | 1 cut(s) | 1447 |
| Hpy188I | TCNGA | 7 cut(s) | 86, 397, 472, 782, 856, 1512, 1617 |
| Hpy188III | TCNNGA | 3 cut(s) | 256, 404, 1732 |
| Hpy99I | CGWCG | 2 cut(s) | 102, 105 |
| HpyAV | CCTTC | 4 cut(s) | 470, 547, 613, 1089 |
| HpyCH4III | ACNGT | 9 cut(s) | 237, 262, 401, 523, 530, 639, 644, 844, 1697 |
| HpyCH4IV | ACGT | 5 cut(s) | 1158, 1407, 1539, 1589, 1736 |
| HpyCH4V | TGCA | 5 cut(s) | 68, 802, 1309, 1516, 1693 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 971, 980, 1424, 1795 |
| HpyF3I | CTNAG | 4 cut(s) | 469, 779, 853, 1389 |
| HpySE526I | ACGT | 5 cut(s) | 1158, 1407, 1539, 1589, 1736 |
| HspAI | GCGC | 1 cut(s) | 1769 |
| Kzo9I | GATC | 5 cut(s) | 104, 511, 712, 1603, 1705 |
| LmnI | GCTCC | 3 cut(s) | 437, 557, 1766 |
| Lsp1109I | GCAGC | 1 cut(s) | 952 |
| LweI | GCATC | 5 cut(s) | 77, 847, 1109, 1503, 1525 |
| MaeI | CTAG | 4 cut(s) | 15, 861, 1727, 1763 |
| MaeII | ACGT | 5 cut(s) | 1158, 1407, 1539, 1589, 1736 |
| MaeIII | GTNAC | 5 cut(s) | 197, 344, 838, 892, 1776 |
| MalI | GATC | 5 cut(s) | 106, 513, 714, 1605, 1707 |
| MboI | GATC | 5 cut(s) | 104, 511, 712, 1603, 1705 |
| MboII | GAAGA | 5 cut(s) | 127, 227, 635, 1450, 1604 |
| MhlI | GDGCHC | 1 cut(s) | 1622 |
| MlyI | GAGTC | 2 cut(s) | 475, 1243 |
| MmeI | TCCRAC | 3 cut(s) | 375, 454, 1625 |
| Mph1103I | ATGCAT | 1 cut(s) | 1518 |
| MroXI | GAANNNNTTC | 1 cut(s) | 651 |
| MseI | TTAA | 8 cut(s) | 8, 75, 122, 372, 744, 881, 929, 1377 |
| MslI | CAYNNNNRTG | 4 cut(s) | 206, 363, 1329, 1521 |
| MspI | CCGG | 4 cut(s) | 762, 906, 984, 1415 |
| MspR9I | CCNGG | 7 cut(s) | 279, 435, 708, 906, 985, 1415, 1677 |
| Mva1269I | GAATGC | 1 cut(s) | 1800 |
| MvaI | CCWGG | 4 cut(s) | 279, 435, 708, 1677 |
| MvnI | CGCG | 1 cut(s) | 1789 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 971, 980, 1424, 1795 |
| NciI | CCSGG | 3 cut(s) | 906, 985, 1415 |
| NcoI | CCATGG | 2 cut(s) | 389, 1522 |
| NdeI | CATATG | 1 cut(s) | 1713 |
| NdeII | GATC | 5 cut(s) | 104, 511, 712, 1603, 1705 |
| NheI | GCTAGC | 1 cut(s) | 860 |
| NlaIV | GGNNCC | 5 cut(s) | 439, 559, 996, 1581, 1724 |
| NmuCI | GTSAC | 2 cut(s) | 838, 892 |
| NsiI | ATGCAT | 1 cut(s) | 1518 |
| NspI | RCATGY | 2 cut(s) | 497, 1210 |
| OliI | CACNNNNGTG | 1 cut(s) | 206 |
| PaeI | GCATGC | 1 cut(s) | 497 |
| PagI | TCATGA | 1 cut(s) | 1731 |
| PctI | GAATGC | 1 cut(s) | 1800 |
| PdmI | GAANNNNTTC | 1 cut(s) | 651 |
| PfeI | GAWTC | 4 cut(s) | 385, 652, 1527, 1659 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 360 |
| PfoI | TCCNGGA | 1 cut(s) | 277 |
| PkrI | GCNGC | 4 cut(s) | 967, 1164, 1261, 1788 |
| Ple19I | CGATCG | 2 cut(s) | 107, 1708 |
| PleI | GAGTC | 2 cut(s) | 475, 1243 |
| PpsI | GAGTC | 2 cut(s) | 475, 1243 |
| Ppu21I | YACGTR | 1 cut(s) | 1408 |
| PpuMI | RGGWCCY | 1 cut(s) | 1579 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1714 |
| Psp5II | RGGWCCY | 1 cut(s) | 1579 |
| Psp6I | CCWGG | 4 cut(s) | 277, 433, 706, 1675 |
| PspFI | CCCAGC | 2 cut(s) | 989, 1235 |
| PspGI | CCWGG | 4 cut(s) | 277, 433, 706, 1675 |
| PspN4I | GGNNCC | 5 cut(s) | 439, 559, 996, 1581, 1724 |
| PspPI | GGNCC | 6 cut(s) | 401, 937, 1238, 1579, 1723, 1759 |
| PspPPI | RGGWCCY | 1 cut(s) | 1579 |
| PvuI | CGATCG | 2 cut(s) | 107, 1708 |
| RsaI | GTAC | 4 cut(s) | 641, 1406, 1410, 1451 |
| RsaNI | GTAC | 4 cut(s) | 640, 1405, 1409, 1450 |
| RseI | CAYNNNNRTG | 4 cut(s) | 206, 363, 1329, 1521 |
| SaqAI | TTAA | 8 cut(s) | 8, 75, 122, 372, 744, 881, 929, 1377 |
| SatI | GCNGC | 4 cut(s) | 966, 1163, 1260, 1787 |
| Sau3AI | GATC | 5 cut(s) | 104, 511, 712, 1603, 1705 |
| Sau96I | GGNCC | 6 cut(s) | 401, 937, 1238, 1579, 1723, 1759 |
| SchI | GAGTC | 2 cut(s) | 475, 1243 |
| ScrFI | CCNGG | 7 cut(s) | 279, 435, 708, 906, 985, 1415, 1677 |
| SduI | GDGCHC | 1 cut(s) | 1622 |
| SfaNI | GCATC | 5 cut(s) | 77, 847, 1109, 1503, 1525 |
| SfcI | CTRYAG | 3 cut(s) | 534, 795, 976 |
| SinI | GGWCC | 3 cut(s) | 401, 937, 1579 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 206, 363, 1329, 1521 |
| SnaBI | TACGTA | 1 cut(s) | 1408 |
| SpeI | ACTAGT | 1 cut(s) | 14 |
| SphI | GCATGC | 1 cut(s) | 497 |
| SsiI | CCGC | 7 cut(s) | 660, 935, 1163, 1260, 1667, 1784, 1787 |
| SspI | AATATT | 1 cut(s) | 1075 |
| SspMI | CTAG | 4 cut(s) | 15, 861, 1727, 1763 |
| StyD4I | CCNGG | 7 cut(s) | 277, 433, 706, 904, 983, 1413, 1675 |
| StyI | CCWWGG | 4 cut(s) | 389, 1036, 1522, 1762 |
| TaaI | ACNGT | 9 cut(s) | 237, 262, 401, 523, 530, 639, 644, 844, 1697 |
| TaiI | ACGT | 5 cut(s) | 1161, 1410, 1542, 1592, 1739 |
| TaqI | TCGA | 6 cut(s) | 103, 107, 213, 514, 1602, 1708 |
| TatI | WGTACW | 2 cut(s) | 639, 1449 |
| TauI | GCSGC | 3 cut(s) | 1165, 1262, 1789 |
| TfiI | GAWTC | 4 cut(s) | 385, 652, 1527, 1659 |
| Tru1I | TTAA | 8 cut(s) | 8, 75, 122, 372, 744, 881, 929, 1377 |
| Tru9I | TTAA | 8 cut(s) | 8, 75, 122, 372, 744, 881, 929, 1377 |
| TscAI | CASTG | 5 cut(s) | 365, 649, 1024, 1270, 1700 |
| TseFI | GTSAC | 2 cut(s) | 838, 892 |
| TseI | GCWGC | 1 cut(s) | 965 |
| Tsp45I | GTSAC | 2 cut(s) | 838, 892 |
| TspDTI | ATGAA | 5 cut(s) | 128, 287, 1041, 1218, 1729 |
| TspGWI | ACGGA | 2 cut(s) | 1189, 1791 |
| TspRI | CASTG | 5 cut(s) | 365, 649, 1024, 1270, 1700 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 360 |
| VpaK11BI | GGWCC | 3 cut(s) | 401, 937, 1579 |
| XapI | RAATTY | 5 cut(s) | 590, 942, 1042, 1357, 1561 |
| XceI | RCATGY | 2 cut(s) | 497, 1210 |
| XmaJI | CCTAGG | 1 cut(s) | 1762 |
| XmiI | GTMKAC | 1 cut(s) | 158 |
| XmnI | GAANNNNTTC | 1 cut(s) | 651 |
| XspI | CTAG | 4 cut(s) | 15, 861, 1727, 1763 |
| Zsp2I | ATGCAT | 1 cut(s) | 1518 |