Lignin degradation and detoxification of lignin-derived products
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 534 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1150 |
| Acc36I | ACCTGC | 1 cut(s) | 675 |
| AccB1I | GGYRCC | 1 cut(s) | 371 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 252 |
| AccI | GTMKAC | 1 cut(s) | 1182 |
| AccII | CGCG | 2 cut(s) | 1060, 1352 |
| AciI | CCGC | 4 cut(s) | 912, 1058, 1259, 1679 |
| AclWI | GGATC | 2 cut(s) | 411, 1608 |
| AcoI | YGGCCR | 3 cut(s) | 6, 160, 799 |
| AcsI | RAATTY | 5 cut(s) | 482, 1252, 1381, 1456, 1668 |
| AcuI | CTGAAG | 2 cut(s) | 384, 1361 |
| AdeI | CACNNNGTG | 1 cut(s) | 312 |
| AfaI | GTAC | 2 cut(s) | 1301, 1346 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 252, 381, 446, 1318, 1319, 1535, 1685 |
| AflIII | ACRYGT | 1 cut(s) | 1145 |
| AgsI | TTSAA | 5 cut(s) | 548, 637, 951, 1511, 1558 |
| AjiI | CACGTC | 3 cut(s) | 1054, 1148, 1627 |
| AjnI | CCWGG | 5 cut(s) | 169, 325, 598, 1570, 1684 |
| AleI | CACNNNNGTG | 2 cut(s) | 98, 737 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 1231, 1263, 1616, 1648 |
| Alw26I | GTCTC | 2 cut(s) | 104, 1022 |
| AlwI | GGATC | 2 cut(s) | 411, 1608 |
| Ama87I | CYCGRG | 1 cut(s) | 193 |
| AoxI | GGCC | 4 cut(s) | 6, 160, 799, 1133 |
| ApaI | GGGCCC | 1 cut(s) | 1137 |
| ApeKI | GCWGC | 2 cut(s) | 333, 857 |
| ApoI | RAATTY | 5 cut(s) | 482, 1252, 1381, 1456, 1668 |
| AspLEI | GCGC | 2 cut(s) | 425, 1062 |
| AspS9I | GGNCC | 5 cut(s) | 173, 293, 1133, 1134, 1474 |
| AsuC2I | CCSGG | 1 cut(s) | 798 |
| AsuHPI | GGTGA | 2 cut(s) | 75, 786 |
| AsuNHI | GCTAGC | 2 cut(s) | 752, 1157 |
| AvaI | CYCGRG | 1 cut(s) | 193 |
| AvaII | GGWCC | 3 cut(s) | 173, 293, 1474 |
| BaeGI | GKGCMC | 2 cut(s) | 317, 1137 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1328, 1361 |
| BalI | TGGCCA | 1 cut(s) | 8 |
| BanI | GGYRCC | 1 cut(s) | 371 |
| BanII | GRGCYC | 4 cut(s) | 746, 839, 1137, 1666 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1320, 1352 |
| BbsI | GAAGAC | 2 cut(s) | 130, 665 |
| BbvI | GCAGC | 2 cut(s) | 345, 844 |
| BccI | CCATC | 5 cut(s) | 254, 637, 1437, 1526, 1602 |
| BceAI | ACGGC | 3 cut(s) | 175, 436, 1296 |
| BciT130I | CCWGG | 5 cut(s) | 171, 327, 600, 1572, 1686 |
| BclI | TGATCA | 1 cut(s) | 1033 |
| BcnI | CCSGG | 1 cut(s) | 798 |
| BcoDI | GTCTC | 2 cut(s) | 104, 1022 |
| BfaI | CTAG | 4 cut(s) | 753, 1028, 1158, 1658 |
| BfmI | CTRYAG | 2 cut(s) | 130, 426 |
| BfoI | RGCGCY | 1 cut(s) | 426 |
| BfuAI | ACCTGC | 1 cut(s) | 675 |
| BisI | GCNGC | 3 cut(s) | 334, 858, 1058 |
| BlsI | GCNGC | 3 cut(s) | 335, 859, 1059 |
| Bme1390I | CCNGG | 6 cut(s) | 171, 327, 600, 798, 1572, 1686 |
| Bme18I | GGWCC | 3 cut(s) | 173, 293, 1474 |
| BmeT110I | CYCGRG | 1 cut(s) | 193 |
| BmgBI | CACGTC | 3 cut(s) | 1054, 1148, 1627 |
| BmgT120I | GGNCC | 5 cut(s) | 173, 293, 1133, 1134, 1474 |
| BmiI | GGNNCC | 6 cut(s) | 373, 451, 561, 1135, 1476, 1663 |
| BmrFI | CCNGG | 6 cut(s) | 171, 327, 600, 798, 1572, 1686 |
| BmsI | GCATC | 4 cut(s) | 72, 739, 1004, 1420 |
| BmtI | GCTAGC | 2 cut(s) | 756, 1161 |
| BpiI | GAAGAC | 2 cut(s) | 130, 665 |
| BpmI | CTGGAG | 1 cut(s) | 1593 |
| Bpu10I | CCTNAGC | 2 cut(s) | 671, 1284 |
| BpuMI | CCSGG | 1 cut(s) | 798 |
| BsaBI | GATNNNNATC | 1 cut(s) | 609 |
| BsaJI | CCNNGG | 4 cut(s) | 281, 418, 1356, 1417 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1352, 1382 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 252, 381, 446, 1318, 1319, 1535, 1685 |
| Bse1I | ACTGG | 4 cut(s) | 163, 257, 573, 1015 |
| Bse3DI | GCAATG | 2 cut(s) | 70, 852 |
| Bse8I | GATNNNNATC | 1 cut(s) | 609 |
| BseBI | CCWGG | 5 cut(s) | 171, 327, 600, 1572, 1686 |
| BseDI | CCNNGG | 4 cut(s) | 281, 418, 1356, 1417 |
| BseGI | GGATG | 3 cut(s) | 246, 1402, 1448 |
| BseJI | GATNNNNATC | 1 cut(s) | 609 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 252, 381, 446, 1318, 1319, 1535, 1685 |
| BseMI | GCAATG | 2 cut(s) | 70, 852 |
| BseMII | CTCAG | 3 cut(s) | 759, 1033, 1298 |
| BseNI | ACTGG | 4 cut(s) | 163, 257, 573, 1015 |
| BseRI | GAGGAG | 1 cut(s) | 1494 |
| BseSI | GKGCMC | 2 cut(s) | 317, 1137 |
| BseXI | GCAGC | 2 cut(s) | 345, 844 |
| BseYI | CCCAGC | 2 cut(s) | 881, 1187 |
| BsgI | GTGCAG | 1 cut(s) | 1149 |
| Bsh1236I | CGCG | 2 cut(s) | 1060, 1352 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1356 |
| BshFI | GGCC | 4 cut(s) | 8, 162, 801, 1135 |
| BshNI | GGYRCC | 1 cut(s) | 371 |
| BsiEI | CGRYCG | 1 cut(s) | 1356 |
| BsiHKCI | CYCGRG | 1 cut(s) | 193 |
| BsiSI | CCGG | 1 cut(s) | 798 |
| BslFI | GGGAC | 5 cut(s) | 1156, 1336, 1390, 1461, 1511 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 252, 381, 446, 1318, 1319, 1535, 1685 |
| BsmAI | GTCTC | 2 cut(s) | 104, 1022 |
| BsmFI | GGGAC | 5 cut(s) | 1156, 1336, 1390, 1461, 1511 |
| BsmI | GAATGC | 1 cut(s) | 1695 |
| BsnI | GGCC | 4 cut(s) | 8, 162, 801, 1135 |
| BsoBI | CYCGRG | 1 cut(s) | 193 |
| Bsp120I | GGGCCC | 1 cut(s) | 1133 |
| Bsp1286I | GDGCHC | 5 cut(s) | 317, 746, 839, 1137, 1666 |
| Bsp143I | GATC | 4 cut(s) | 403, 604, 1033, 1600 |
| Bsp19I | CCATGG | 2 cut(s) | 281, 1417 |
| BspACI | CCGC | 4 cut(s) | 912, 1058, 1259, 1679 |
| BspANI | GGCC | 4 cut(s) | 8, 162, 801, 1135 |
| BspCNI | CTCAG | 3 cut(s) | 758, 1032, 1297 |
| BspFNI | CGCG | 2 cut(s) | 1060, 1352 |
| BspLI | GGNNCC | 6 cut(s) | 373, 451, 561, 1135, 1476, 1663 |
| BspMI | ACCTGC | 1 cut(s) | 675 |
| BspOI | GCTAGC | 2 cut(s) | 756, 1161 |
| BspPI | GGATC | 2 cut(s) | 411, 1608 |
| BspT107I | GGYRCC | 1 cut(s) | 371 |
| BsrDI | GCAATG | 2 cut(s) | 70, 852 |
| BsrI | ACTGG | 4 cut(s) | 163, 257, 573, 1015 |
| BssECI | CCNNGG | 4 cut(s) | 281, 418, 1356, 1417 |
| BssMI | GATC | 4 cut(s) | 403, 604, 1033, 1600 |
| BssT1I | CCWWGG | 2 cut(s) | 281, 1417 |
| Bst2UI | CCWGG | 5 cut(s) | 171, 327, 600, 1572, 1686 |
| Bst4CI | ACNGT | 8 cut(s) | 154, 183, 293, 395, 415, 736, 1357, 1549 |
| Bst6I | CTCTTC | 2 cut(s) | 102, 1512 |
| BstC8I | GCNNGC | 5 cut(s) | 29, 387, 754, 1159, 1206 |
| BstDEI | CTNAG | 6 cut(s) | 272, 496, 671, 745, 1019, 1284 |
| BstDSI | CCRYGG | 4 cut(s) | 281, 418, 1356, 1417 |
| BstF5I | GGATG | 3 cut(s) | 246, 1402, 1448 |
| BstFNI | CGCG | 2 cut(s) | 1060, 1352 |
| BstH2I | RGCGCY | 1 cut(s) | 426 |
| BstHHI | GCGC | 2 cut(s) | 425, 1062 |
| BstKTI | GATC | 4 cut(s) | 406, 607, 1036, 1603 |
| BstMAI | GTCTC | 2 cut(s) | 104, 1022 |
| BstMBI | GATC | 4 cut(s) | 403, 604, 1033, 1600 |
| BstMCI | CGRYCG | 1 cut(s) | 1356 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 863 |
| BstNI | CCWGG | 5 cut(s) | 171, 327, 600, 1572, 1686 |
| BstNSI | RCATGY | 1 cut(s) | 389 |
| BstSCI | CCNGG | 6 cut(s) | 169, 325, 598, 796, 1570, 1684 |
| BstSFI | CTRYAG | 2 cut(s) | 130, 426 |
| BstSLI | GKGCMC | 2 cut(s) | 317, 1137 |
| BstUI | CGCG | 2 cut(s) | 1060, 1352 |
| BstV1I | GCAGC | 2 cut(s) | 345, 844 |
| BstV2I | GAAGAC | 2 cut(s) | 130, 665 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 170, 257, 1226 |
| BsuRI | GGCC | 4 cut(s) | 8, 162, 801, 1135 |
| BtgI | CCRYGG | 4 cut(s) | 281, 418, 1356, 1417 |
| BtgZI | GCGATG | 1 cut(s) | 1504 |
| BtrI | CACGTC | 3 cut(s) | 1054, 1148, 1627 |
| BtsCI | GGATG | 3 cut(s) | 246, 1402, 1448 |
| BtsI | GCAGTG | 3 cut(s) | 317, 822, 1588 |
| BtsIMutI | CAGTG | 7 cut(s) | 179, 250, 317, 400, 580, 822, 1588 |
| BveI | ACCTGC | 1 cut(s) | 675 |
| Cac8I | GCNNGC | 5 cut(s) | 29, 387, 754, 1159, 1206 |
| CfoI | GCGC | 2 cut(s) | 425, 1062 |
| Cfr13I | GGNCC | 5 cut(s) | 173, 293, 1133, 1134, 1474 |
| CseI | GACGC | 1 cut(s) | 1150 |
| Csp6I | GTAC | 2 cut(s) | 1300, 1345 |
| CviQI | GTAC | 2 cut(s) | 1300, 1345 |
| DdeI | CTNAG | 6 cut(s) | 272, 496, 671, 745, 1019, 1284 |
| DpnI | GATC | 4 cut(s) | 405, 606, 1035, 1602 |
| DpnII | GATC | 4 cut(s) | 403, 604, 1033, 1600 |
| DraIII | CACNNNGTG | 1 cut(s) | 312 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1150 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1150 |
| EaeI | YGGCCR | 3 cut(s) | 6, 160, 799 |
| Eam1104I | CTCTTC | 2 cut(s) | 102, 1512 |
| EarI | CTCTTC | 2 cut(s) | 102, 1512 |
| EciI | GGCGGA | 1 cut(s) | 1668 |
| Eco130I | CCWWGG | 2 cut(s) | 281, 1417 |
| Eco24I | GRGCYC | 4 cut(s) | 746, 839, 1137, 1666 |
| Eco47I | GGWCC | 3 cut(s) | 173, 293, 1474 |
| Eco57I | CTGAAG | 2 cut(s) | 384, 1361 |
| Eco88I | CYCGRG | 1 cut(s) | 193 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1133, 1474 |
| EcoRII | CCWGG | 5 cut(s) | 169, 325, 598, 1570, 1684 |
| EcoT14I | CCWWGG | 2 cut(s) | 281, 1417 |
| EcoT22I | ATGCAT | 1 cut(s) | 1413 |
| EcoT38I | GRGCYC | 4 cut(s) | 746, 839, 1137, 1666 |
| ErhI | CCWWGG | 2 cut(s) | 281, 1417 |
| FaqI | GGGAC | 5 cut(s) | 1156, 1336, 1390, 1461, 1511 |
| FauI | CCCGC | 1 cut(s) | 1266 |
| FauNDI | CATATG | 1 cut(s) | 1608 |
| FbaI | TGATCA | 1 cut(s) | 1033 |
| FblI | GTMKAC | 1 cut(s) | 1182 |
| Fnu4HI | GCNGC | 3 cut(s) | 334, 858, 1058 |
| FokI | GGATG | 3 cut(s) | 233, 1409, 1455 |
| FriOI | GRGCYC | 4 cut(s) | 746, 839, 1137, 1666 |
| Fsp4HI | GCNGC | 3 cut(s) | 334, 858, 1058 |
| FspBI | CTAG | 4 cut(s) | 753, 1028, 1158, 1658 |
| GlaI | GCGC | 2 cut(s) | 424, 1061 |
| GluI | GCNGC | 3 cut(s) | 334, 858, 1058 |
| GsaI | CCCAGC | 2 cut(s) | 885, 1191 |
| GsuI | CTGGAG | 1 cut(s) | 1593 |
| HaeII | RGCGCY | 1 cut(s) | 426 |
| HaeIII | GGCC | 4 cut(s) | 8, 162, 801, 1135 |
| HapII | CCGG | 1 cut(s) | 798 |
| HgaI | GACGC | 1 cut(s) | 1150 |
| HhaI | GCGC | 2 cut(s) | 425, 1062 |
| Hin6I | GCGC | 2 cut(s) | 423, 1060 |
| HinP1I | GCGC | 2 cut(s) | 423, 1060 |
| HincII | GTYRAC | 6 cut(s) | 596, 1051, 1099, 1165, 1183, 1498 |
| HindII | GTYRAC | 6 cut(s) | 596, 1051, 1099, 1165, 1183, 1498 |
| HindIII | AAGCTT | 1 cut(s) | 78 |
| HinfI | GANTC | 5 cut(s) | 112, 126, 277, 1422, 1554 |
| HpaII | CCGG | 1 cut(s) | 798 |
| HphI | GGTGA | 2 cut(s) | 75, 786 |
| Hpy188I | TCNGA | 6 cut(s) | 289, 364, 748, 778, 1022, 1251 |
| Hpy188III | TCNNGA | 5 cut(s) | 195, 296, 712, 1286, 1598 |
| Hpy99I | CGWCG | 1 cut(s) | 215 |
| HpyAV | CCTTC | 4 cut(s) | 362, 505, 966, 984 |
| HpyCH4III | ACNGT | 8 cut(s) | 154, 183, 293, 395, 415, 736, 1357, 1549 |
| HpyCH4IV | ACGT | 6 cut(s) | 47, 870, 1053, 1147, 1626, 1631 |
| HpyCH4V | TGCA | 8 cut(s) | 31, 479, 694, 1130, 1204, 1391, 1411, 1588 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 863 |
| HpyF3I | CTNAG | 6 cut(s) | 272, 496, 671, 745, 1019, 1284 |
| HpySE526I | ACGT | 6 cut(s) | 47, 870, 1053, 1147, 1626, 1631 |
| HspAI | GCGC | 2 cut(s) | 423, 1060 |
| Ksp22I | TGATCA | 1 cut(s) | 1033 |
| Kzo9I | GATC | 4 cut(s) | 403, 604, 1033, 1600 |
| LmnI | GCTCC | 2 cut(s) | 449, 1661 |
| Lsp1109I | GCAGC | 2 cut(s) | 345, 844 |
| LweI | GCATC | 4 cut(s) | 72, 739, 1004, 1420 |
| MaeI | CTAG | 4 cut(s) | 753, 1028, 1158, 1658 |
| MaeII | ACGT | 6 cut(s) | 47, 870, 1053, 1147, 1626, 1631 |
| MaeIII | GTNAC | 5 cut(s) | 89, 236, 730, 784, 1627 |
| MalI | GATC | 4 cut(s) | 405, 606, 1035, 1602 |
| MboI | GATC | 4 cut(s) | 403, 604, 1033, 1600 |
| MboII | GAAGA | 9 cut(s) | 58, 119, 135, 377, 527, 670, 962, 1345, 1499 |
| MhlI | GDGCHC | 5 cut(s) | 317, 746, 839, 1137, 1666 |
| MlsI | TGGCCA | 1 cut(s) | 8 |
| MluNI | TGGCCA | 1 cut(s) | 8 |
| MlyI | GAGTC | 2 cut(s) | 106, 120 |
| MmeI | TCCRAC | 2 cut(s) | 267, 346 |
| Mox20I | TGGCCA | 1 cut(s) | 8 |
| Mph1103I | ATGCAT | 1 cut(s) | 1413 |
| MscI | TGGCCA | 1 cut(s) | 8 |
| MseI | TTAA | 3 cut(s) | 20, 1272, 1702 |
| MslI | CAYNNNNRTG | 7 cut(s) | 98, 255, 737, 1108, 1224, 1416, 1625 |
| Msp20I | TGGCCA | 1 cut(s) | 8 |
| MspA1I | CMGCKG | 1 cut(s) | 689 |
| MspI | CCGG | 1 cut(s) | 798 |
| MspR9I | CCNGG | 6 cut(s) | 171, 327, 600, 798, 1572, 1686 |
| Mva1269I | GAATGC | 1 cut(s) | 1695 |
| MvaI | CCWGG | 5 cut(s) | 171, 327, 600, 1572, 1686 |
| MvnI | CGCG | 2 cut(s) | 1060, 1352 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 863 |
| NciI | CCSGG | 1 cut(s) | 798 |
| NcoI | CCATGG | 2 cut(s) | 281, 1417 |
| NdeI | CATATG | 1 cut(s) | 1608 |
| NdeII | GATC | 4 cut(s) | 403, 604, 1033, 1600 |
| NheI | GCTAGC | 2 cut(s) | 752, 1157 |
| NlaIV | GGNNCC | 6 cut(s) | 373, 451, 561, 1135, 1476, 1663 |
| NmuCI | GTSAC | 3 cut(s) | 730, 784, 1627 |
| NsiI | ATGCAT | 1 cut(s) | 1413 |
| NspI | RCATGY | 1 cut(s) | 389 |
| OliI | CACNNNNGTG | 2 cut(s) | 98, 737 |
| PaeI | GCATGC | 1 cut(s) | 389 |
| PctI | GAATGC | 1 cut(s) | 1695 |
| PfeI | GAWTC | 3 cut(s) | 277, 1422, 1554 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 252 |
| PfoI | TCCNGGA | 2 cut(s) | 169, 1570 |
| PkrI | GCNGC | 3 cut(s) | 335, 859, 1059 |
| PleI | GAGTC | 2 cut(s) | 106, 120 |
| PpsI | GAGTC | 2 cut(s) | 106, 120 |
| PpuMI | RGGWCCY | 1 cut(s) | 1474 |
| PsiI | TTATAA | 1 cut(s) | 534 |
| Psp5II | RGGWCCY | 1 cut(s) | 1474 |
| Psp6I | CCWGG | 5 cut(s) | 169, 325, 598, 1570, 1684 |
| PspFI | CCCAGC | 2 cut(s) | 881, 1187 |
| PspGI | CCWGG | 5 cut(s) | 169, 325, 598, 1570, 1684 |
| PspN4I | GGNNCC | 6 cut(s) | 373, 451, 561, 1135, 1476, 1663 |
| PspOMI | GGGCCC | 1 cut(s) | 1133 |
| PspPI | GGNCC | 5 cut(s) | 173, 293, 1133, 1134, 1474 |
| PspPPI | RGGWCCY | 1 cut(s) | 1474 |
| PvuII | CAGCTG | 1 cut(s) | 689 |
| RsaI | GTAC | 2 cut(s) | 1301, 1346 |
| RsaNI | GTAC | 2 cut(s) | 1300, 1345 |
| RseI | CAYNNNNRTG | 7 cut(s) | 98, 255, 737, 1108, 1224, 1416, 1625 |
| SalI | GTCGAC | 1 cut(s) | 1181 |
| SaqAI | TTAA | 3 cut(s) | 20, 1272, 1702 |
| SatI | GCNGC | 3 cut(s) | 334, 858, 1058 |
| Sau3AI | GATC | 4 cut(s) | 403, 604, 1033, 1600 |
| Sau96I | GGNCC | 5 cut(s) | 173, 293, 1133, 1134, 1474 |
| SchI | GAGTC | 2 cut(s) | 106, 120 |
| ScrFI | CCNGG | 6 cut(s) | 171, 327, 600, 798, 1572, 1686 |
| SduI | GDGCHC | 5 cut(s) | 317, 746, 839, 1137, 1666 |
| SfaNI | GCATC | 4 cut(s) | 72, 739, 1004, 1420 |
| SfcI | CTRYAG | 2 cut(s) | 130, 426 |
| SinI | GGWCC | 3 cut(s) | 173, 293, 1474 |
| SmiMI | CAYNNNNRTG | 7 cut(s) | 98, 255, 737, 1108, 1224, 1416, 1625 |
| SphI | GCATGC | 1 cut(s) | 389 |
| SsiI | CCGC | 4 cut(s) | 912, 1058, 1259, 1679 |
| SspMI | CTAG | 4 cut(s) | 753, 1028, 1158, 1658 |
| StyD4I | CCNGG | 6 cut(s) | 169, 325, 598, 796, 1570, 1684 |
| StyI | CCWWGG | 2 cut(s) | 281, 1417 |
| TaaI | ACNGT | 8 cut(s) | 154, 183, 293, 395, 415, 736, 1357, 1549 |
| TaiI | ACGT | 6 cut(s) | 50, 873, 1056, 1150, 1629, 1634 |
| TaqI | TCGA | 5 cut(s) | 213, 406, 713, 1182, 1243 |
| TatI | WGTACW | 2 cut(s) | 1299, 1344 |
| TauI | GCSGC | 1 cut(s) | 1060 |
| TfiI | GAWTC | 3 cut(s) | 277, 1422, 1554 |
| Tru1I | TTAA | 3 cut(s) | 20, 1272, 1702 |
| Tru9I | TTAA | 3 cut(s) | 20, 1272, 1702 |
| TscAI | CASTG | 7 cut(s) | 186, 257, 317, 400, 580, 829, 1595 |
| TseFI | GTSAC | 3 cut(s) | 730, 784, 1627 |
| TseI | GCWGC | 2 cut(s) | 333, 857 |
| Tsp45I | GTSAC | 3 cut(s) | 730, 784, 1627 |
| TspDTI | ATGAA | 4 cut(s) | 762, 992, 1113, 1624 |
| TspGWI | ACGGA | 1 cut(s) | 1340 |
| TspRI | CASTG | 7 cut(s) | 186, 257, 317, 400, 580, 829, 1595 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 252 |
| VpaK11BI | GGWCC | 3 cut(s) | 173, 293, 1474 |
| XapI | RAATTY | 5 cut(s) | 482, 1252, 1381, 1456, 1668 |
| XceI | RCATGY | 1 cut(s) | 389 |
| XmiI | GTMKAC | 1 cut(s) | 1182 |
| XspI | CTAG | 4 cut(s) | 753, 1028, 1158, 1658 |
| Zsp2I | ATGCAT | 1 cut(s) | 1413 |