Lignin degradation and detoxification of lignin-derived products
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 2 cut(s) | 88, 936 |
| AccIII | TCCGGA | 1 cut(s) | 551 |
| AciI | CCGC | 7 cut(s) | 576, 687, 858, 936, 1173, 1277, 1313 |
| AclWI | GGATC | 2 cut(s) | 411, 1511 |
| AcoI | YGGCCR | 3 cut(s) | 80, 601, 855 |
| AcsI | RAATTY | 1 cut(s) | 1250 |
| AcuI | CTGAAG | 1 cut(s) | 1307 |
| AdeI | CACNNNGTG | 1 cut(s) | 312 |
| AfaI | GTAC | 4 cut(s) | 185, 417, 1319, 1364 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 445, 1149, 1337, 1553 |
| AflIII | ACRYGT | 1 cut(s) | 1163 |
| AgsI | TTSAA | 6 cut(s) | 323, 548, 637, 1391, 1501, 1576 |
| AjnI | CCWGG | 3 cut(s) | 325, 796, 1588 |
| AleI | CACNNNNGTG | 2 cut(s) | 98, 1373 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1249, 1281 |
| AluBI | AGCT | 8 cut(s) | 77, 529, 756, 1044, 1049, 1194, 1226, 1381 |
| AluI | AGCT | 8 cut(s) | 77, 529, 756, 1044, 1049, 1194, 1226, 1381 |
| Alw26I | GTCTC | 4 cut(s) | 192, 623, 1040, 1114 |
| AlwI | GGATC | 2 cut(s) | 411, 1511 |
| Aor13HI | TCCGGA | 1 cut(s) | 551 |
| AoxI | GGCC | 6 cut(s) | 80, 601, 855, 1151, 1578, 1672 |
| ApaI | GGGCCC | 1 cut(s) | 1676 |
| ApoI | RAATTY | 1 cut(s) | 1250 |
| AseI | ATTAAT | 1 cut(s) | 51 |
| AspLEI | GCGC | 3 cut(s) | 88, 1148, 1303 |
| AspS9I | GGNCC | 9 cut(s) | 173, 293, 559, 1151, 1201, 1492, 1642, 1672, 1673 |
| AsuC2I | CCSGG | 3 cut(s) | 171, 600, 1150 |
| AsuHPI | GGTGA | 3 cut(s) | 331, 786, 1360 |
| AsuNHI | GCTAGC | 2 cut(s) | 752, 1175 |
| AvaII | GGWCC | 6 cut(s) | 173, 293, 559, 1201, 1492, 1642 |
| BaeGI | GKGCMC | 2 cut(s) | 317, 1676 |
| BalI | TGGCCA | 1 cut(s) | 82 |
| BanII | GRGCYC | 1 cut(s) | 1676 |
| BccI | CCATC | 5 cut(s) | 612, 637, 722, 1428, 1620 |
| BceAI | ACGGC | 1 cut(s) | 1314 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 35 |
| BciT130I | CCWGG | 3 cut(s) | 327, 798, 1590 |
| BciVI | GTATCC | 1 cut(s) | 621 |
| BcnI | CCSGG | 3 cut(s) | 171, 600, 1150 |
| BcoDI | GTCTC | 4 cut(s) | 192, 623, 1040, 1114 |
| BfaI | CTAG | 3 cut(s) | 753, 1176, 1206 |
| BfuI | GTATCC | 1 cut(s) | 621 |
| BisI | GCNGC | 3 cut(s) | 858, 1173, 1313 |
| BlpI | GCTNAGC | 1 cut(s) | 1045 |
| BlsI | GCNGC | 3 cut(s) | 859, 1174, 1314 |
| Bme1390I | CCNGG | 6 cut(s) | 171, 327, 600, 798, 1150, 1590 |
| Bme18I | GGWCC | 6 cut(s) | 173, 293, 559, 1201, 1492, 1642 |
| BmgT120I | GGNCC | 9 cut(s) | 173, 293, 559, 1151, 1201, 1492, 1642, 1672, 1673 |
| BmiI | GGNNCC | 7 cut(s) | 331, 560, 561, 888, 1203, 1494, 1674 |
| BmrFI | CCNGG | 6 cut(s) | 171, 327, 600, 798, 1150, 1590 |
| BmsI | GCATC | 4 cut(s) | 66, 739, 1022, 1438 |
| BmtI | GCTAGC | 2 cut(s) | 756, 1179 |
| BpmI | CTGGAG | 1 cut(s) | 1611 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1045 |
| BpuEI | CTTGAG | 1 cut(s) | 1662 |
| BpuMI | CCSGG | 3 cut(s) | 171, 600, 1150 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1419 |
| BsaJI | CCNNGG | 5 cut(s) | 272, 281, 360, 1371, 1588 |
| BsaWI | WCCGGW | 2 cut(s) | 551, 1702 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 189, 219, 949, 979, 1094, 1124 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 445, 1149, 1337, 1553 |
| Bse1I | ACTGG | 1 cut(s) | 257 |
| Bse3DI | GCAATG | 1 cut(s) | 67 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1419 |
| BseAI | TCCGGA | 1 cut(s) | 551 |
| BseBI | CCWGG | 3 cut(s) | 327, 798, 1590 |
| BseDI | CCNNGG | 5 cut(s) | 272, 281, 360, 1371, 1588 |
| BseGI | GGATG | 3 cut(s) | 57, 1420, 1420 |
| BseJI | GATNNNNATC | 1 cut(s) | 1419 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 445, 1149, 1337, 1553 |
| BseMI | GCAATG | 1 cut(s) | 67 |
| BseMII | CTCAG | 1 cut(s) | 1051 |
| BseNI | ACTGG | 1 cut(s) | 257 |
| BseRI | GAGGAG | 3 cut(s) | 53, 377, 1512 |
| BseSI | GKGCMC | 2 cut(s) | 317, 1676 |
| Bsh1236I | CGCG | 2 cut(s) | 88, 936 |
| BshFI | GGCC | 6 cut(s) | 82, 603, 857, 1153, 1580, 1674 |
| BsiSI | CCGG | 6 cut(s) | 171, 552, 600, 1029, 1149, 1703 |
| BslFI | GGGAC | 5 cut(s) | 572, 1174, 1354, 1408, 1529 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 445, 1149, 1337, 1553 |
| BsmAI | GTCTC | 4 cut(s) | 192, 623, 1040, 1114 |
| BsmFI | GGGAC | 5 cut(s) | 572, 1174, 1354, 1408, 1529 |
| BsnI | GGCC | 6 cut(s) | 82, 603, 857, 1153, 1580, 1674 |
| Bsp120I | GGGCCC | 1 cut(s) | 1672 |
| Bsp1286I | GDGCHC | 2 cut(s) | 317, 1676 |
| Bsp13I | TCCGGA | 1 cut(s) | 551 |
| Bsp1407I | TGTACA | 1 cut(s) | 415 |
| Bsp143I | GATC | 3 cut(s) | 403, 489, 1516 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1045 |
| Bsp19I | CCATGG | 1 cut(s) | 281 |
| BspACI | CCGC | 7 cut(s) | 576, 687, 858, 936, 1173, 1277, 1313 |
| BspANI | GGCC | 6 cut(s) | 82, 603, 857, 1153, 1580, 1674 |
| BspCNI | CTCAG | 1 cut(s) | 1050 |
| BspEI | TCCGGA | 1 cut(s) | 551 |
| BspFNI | CGCG | 2 cut(s) | 88, 936 |
| BspLI | GGNNCC | 7 cut(s) | 331, 560, 561, 888, 1203, 1494, 1674 |
| BspOI | GCTAGC | 2 cut(s) | 756, 1179 |
| BspPI | GGATC | 2 cut(s) | 411, 1511 |
| BsrDI | GCAATG | 1 cut(s) | 67 |
| BsrGI | TGTACA | 1 cut(s) | 415 |
| BsrI | ACTGG | 1 cut(s) | 257 |
| BssECI | CCNNGG | 5 cut(s) | 272, 281, 360, 1371, 1588 |
| BssMI | GATC | 3 cut(s) | 403, 489, 1516 |
| BssT1I | CCWWGG | 2 cut(s) | 272, 281 |
| Bst2UI | CCWGG | 3 cut(s) | 327, 798, 1590 |
| Bst6I | CTCTTC | 3 cut(s) | 549, 993, 1530 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1309 |
| BstAUI | TGTACA | 1 cut(s) | 415 |
| BstC8I | GCNNGC | 6 cut(s) | 20, 84, 190, 387, 754, 1177 |
| BstDEI | CTNAG | 3 cut(s) | 671, 1037, 1045 |
| BstDSI | CCRYGG | 2 cut(s) | 281, 1371 |
| BstF5I | GGATG | 3 cut(s) | 57, 1420, 1420 |
| BstFNI | CGCG | 2 cut(s) | 88, 936 |
| BstHHI | GCGC | 3 cut(s) | 88, 1148, 1303 |
| BstKTI | GATC | 3 cut(s) | 406, 492, 1519 |
| BstMAI | GTCTC | 4 cut(s) | 192, 623, 1040, 1114 |
| BstMBI | GATC | 3 cut(s) | 403, 489, 1516 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 83, 863, 1309 |
| BstNI | CCWGG | 3 cut(s) | 327, 798, 1590 |
| BstNSI | RCATGY | 1 cut(s) | 389 |
| BstSCI | CCNGG | 6 cut(s) | 169, 325, 598, 796, 1148, 1588 |
| BstSLI | GKGCMC | 2 cut(s) | 317, 1676 |
| BstUI | CGCG | 2 cut(s) | 88, 936 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 257 |
| BsuI | GTATCC | 1 cut(s) | 621 |
| BsuRI | GGCC | 6 cut(s) | 82, 603, 857, 1153, 1580, 1674 |
| BtgI | CCRYGG | 2 cut(s) | 281, 1371 |
| BtgZI | GCGATG | 1 cut(s) | 700 |
| BtsCI | GGATG | 3 cut(s) | 57, 1420, 1420 |
| BtsI | GCAGTG | 3 cut(s) | 317, 822, 1606 |
| BtsIMutI | CAGTG | 6 cut(s) | 250, 317, 400, 411, 822, 1606 |
| Cac8I | GCNNGC | 6 cut(s) | 20, 84, 190, 387, 754, 1177 |
| CfoI | GCGC | 3 cut(s) | 88, 1148, 1303 |
| Cfr13I | GGNCC | 9 cut(s) | 173, 293, 559, 1151, 1201, 1492, 1642, 1672, 1673 |
| CpoI | CGGWCCG | 1 cut(s) | 173 |
| CsiI | ACCWGGT | 1 cut(s) | 796 |
| Csp6I | GTAC | 4 cut(s) | 184, 416, 1318, 1363 |
| CspI | CGGWCCG | 1 cut(s) | 173 |
| CviQI | GTAC | 4 cut(s) | 184, 416, 1318, 1363 |
| DdeI | CTNAG | 3 cut(s) | 671, 1037, 1045 |
| DpnI | GATC | 3 cut(s) | 405, 491, 1518 |
| DpnII | GATC | 3 cut(s) | 403, 489, 1516 |
| DraIII | CACNNNGTG | 1 cut(s) | 312 |
| EaeI | YGGCCR | 3 cut(s) | 80, 601, 855 |
| Eam1104I | CTCTTC | 3 cut(s) | 549, 993, 1530 |
| EarI | CTCTTC | 3 cut(s) | 549, 993, 1530 |
| Eco130I | CCWWGG | 2 cut(s) | 272, 281 |
| Eco24I | GRGCYC | 1 cut(s) | 1676 |
| Eco47I | GGWCC | 6 cut(s) | 173, 293, 559, 1201, 1492, 1642 |
| Eco57I | CTGAAG | 1 cut(s) | 1307 |
| EcoO109I | RGGNCCY | 4 cut(s) | 559, 1492, 1672, 1673 |
| EcoRII | CCWGG | 3 cut(s) | 325, 796, 1588 |
| EcoT14I | CCWWGG | 2 cut(s) | 272, 281 |
| EcoT22I | ATGCAT | 2 cut(s) | 1015, 1431 |
| EcoT38I | GRGCYC | 1 cut(s) | 1676 |
| ErhI | CCWWGG | 2 cut(s) | 272, 281 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 131, 163, 360, 392 |
| FaqI | GGGAC | 5 cut(s) | 572, 1174, 1354, 1408, 1529 |
| FauI | CCCGC | 2 cut(s) | 929, 1284 |
| FauNDI | CATATG | 1 cut(s) | 1626 |
| Fnu4HI | GCNGC | 3 cut(s) | 858, 1173, 1313 |
| FokI | GGATG | 3 cut(s) | 44, 1407, 1427 |
| FriOI | GRGCYC | 1 cut(s) | 1676 |
| Fsp4HI | GCNGC | 3 cut(s) | 858, 1173, 1313 |
| FspBI | CTAG | 3 cut(s) | 753, 1176, 1206 |
| GlaI | GCGC | 3 cut(s) | 87, 1147, 1302 |
| GluI | GCNGC | 3 cut(s) | 858, 1173, 1313 |
| GsuI | CTGGAG | 1 cut(s) | 1611 |
| HaeIII | GGCC | 6 cut(s) | 82, 603, 857, 1153, 1580, 1674 |
| HapII | CCGG | 6 cut(s) | 171, 552, 600, 1029, 1149, 1703 |
| HhaI | GCGC | 3 cut(s) | 88, 1148, 1303 |
| Hin6I | GCGC | 3 cut(s) | 86, 1146, 1301 |
| HinP1I | GCGC | 3 cut(s) | 86, 1146, 1301 |
| HincII | GTYRAC | 4 cut(s) | 265, 596, 1117, 1243 |
| HindII | GTYRAC | 4 cut(s) | 265, 596, 1117, 1243 |
| HindIII | AAGCTT | 4 cut(s) | 75, 527, 1042, 1224 |
| HinfI | GANTC | 5 cut(s) | 277, 319, 623, 1327, 1572 |
| HpaII | CCGG | 6 cut(s) | 171, 552, 600, 1029, 1149, 1703 |
| HphI | GGTGA | 3 cut(s) | 331, 786, 1360 |
| Hpy166II | GTNNAC | 7 cut(s) | 265, 596, 1117, 1183, 1201, 1243, 1560 |
| Hpy188III | TCNNGA | 5 cut(s) | 148, 487, 552, 1268, 1616 |
| Hpy8I | GTNNAC | 7 cut(s) | 265, 596, 1117, 1183, 1201, 1243, 1560 |
| Hpy99I | CGWCG | 1 cut(s) | 215 |
| HpyAV | CCTTC | 6 cut(s) | 362, 505, 972, 984, 1331, 1345 |
| HpyCH4IV | ACGT | 1 cut(s) | 1165 |
| HpyCH4V | TGCA | 9 cut(s) | 22, 425, 479, 694, 922, 1013, 1429, 1435, 1606 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 83, 863, 1309 |
| HpyF3I | CTNAG | 3 cut(s) | 671, 1037, 1045 |
| HpySE526I | ACGT | 1 cut(s) | 1165 |
| HspAI | GCGC | 3 cut(s) | 86, 1146, 1301 |
| KflI | GGGWCCC | 1 cut(s) | 559 |
| Kpn2I | TCCGGA | 1 cut(s) | 551 |
| Kzo9I | GATC | 3 cut(s) | 403, 489, 1516 |
| LmnI | GCTCC | 2 cut(s) | 329, 1378 |
| LweI | GCATC | 4 cut(s) | 66, 739, 1022, 1438 |
| MabI | ACCWGGT | 1 cut(s) | 796 |
| MaeI | CTAG | 3 cut(s) | 753, 1176, 1206 |
| MaeII | ACGT | 1 cut(s) | 1165 |
| MaeIII | GTNAC | 6 cut(s) | 89, 236, 730, 784, 1439, 1689 |
| MalI | GATC | 3 cut(s) | 405, 491, 1518 |
| MboI | GATC | 3 cut(s) | 403, 489, 1516 |
| MboII | GAAGA | 6 cut(s) | 16, 119, 527, 536, 980, 1517 |
| MhlI | GDGCHC | 2 cut(s) | 317, 1676 |
| MlsI | TGGCCA | 1 cut(s) | 82 |
| MluNI | TGGCCA | 1 cut(s) | 82 |
| MmeI | TCCRAC | 3 cut(s) | 267, 992, 1438 |
| Mox20I | TGGCCA | 1 cut(s) | 82 |
| Mph1103I | ATGCAT | 2 cut(s) | 1015, 1431 |
| MroI | TCCGGA | 1 cut(s) | 551 |
| MscI | TGGCCA | 1 cut(s) | 82 |
| MseI | TTAA | 8 cut(s) | 51, 222, 588, 941, 1020, 1080, 1290, 1720 |
| MslI | CAYNNNNRTG | 4 cut(s) | 98, 255, 1373, 1434 |
| Msp20I | TGGCCA | 1 cut(s) | 82 |
| MspA1I | CMGCKG | 2 cut(s) | 689, 1315 |
| MspI | CCGG | 6 cut(s) | 171, 552, 600, 1029, 1149, 1703 |
| MspR9I | CCNGG | 6 cut(s) | 171, 327, 600, 798, 1150, 1590 |
| MvaI | CCWGG | 3 cut(s) | 327, 798, 1590 |
| MvnI | CGCG | 2 cut(s) | 88, 936 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 83, 863, 1309 |
| NciI | CCSGG | 3 cut(s) | 171, 600, 1150 |
| NcoI | CCATGG | 1 cut(s) | 281 |
| NdeI | CATATG | 1 cut(s) | 1626 |
| NdeII | GATC | 3 cut(s) | 403, 489, 1516 |
| NheI | GCTAGC | 2 cut(s) | 752, 1175 |
| NlaIV | GGNNCC | 7 cut(s) | 331, 560, 561, 888, 1203, 1494, 1674 |
| NmuCI | GTSAC | 2 cut(s) | 730, 784 |
| NsiI | ATGCAT | 2 cut(s) | 1015, 1431 |
| NspI | RCATGY | 1 cut(s) | 389 |
| OliI | CACNNNNGTG | 2 cut(s) | 98, 1373 |
| PaeI | GCATGC | 1 cut(s) | 389 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 314, 1176 |
| PfeI | GAWTC | 5 cut(s) | 277, 319, 623, 1327, 1572 |
| PflFI | GACNNNGTC | 1 cut(s) | 203 |
| PfoI | TCCNGGA | 1 cut(s) | 169 |
| PkrI | GCNGC | 3 cut(s) | 859, 1174, 1314 |
| PpuMI | RGGWCCY | 2 cut(s) | 559, 1492 |
| PshBI | ATTAAT | 1 cut(s) | 51 |
| Psp5II | RGGWCCY | 2 cut(s) | 559, 1492 |
| Psp6I | CCWGG | 3 cut(s) | 325, 796, 1588 |
| PspGI | CCWGG | 3 cut(s) | 325, 796, 1588 |
| PspN4I | GGNNCC | 7 cut(s) | 331, 560, 561, 888, 1203, 1494, 1674 |
| PspOMI | GGGCCC | 1 cut(s) | 1672 |
| PspPI | GGNCC | 9 cut(s) | 173, 293, 559, 1151, 1201, 1492, 1642, 1672, 1673 |
| PspPPI | RGGWCCY | 2 cut(s) | 559, 1492 |
| PsyI | GACNNNGTC | 1 cut(s) | 203 |
| RsaI | GTAC | 4 cut(s) | 185, 417, 1319, 1364 |
| RsaNI | GTAC | 4 cut(s) | 184, 416, 1318, 1363 |
| RseI | CAYNNNNRTG | 4 cut(s) | 98, 255, 1373, 1434 |
| Rsr2I | CGGWCCG | 1 cut(s) | 173 |
| RsrII | CGGWCCG | 1 cut(s) | 173 |
| SaqAI | TTAA | 8 cut(s) | 51, 222, 588, 941, 1020, 1080, 1290, 1720 |
| SatI | GCNGC | 3 cut(s) | 858, 1173, 1313 |
| Sau3AI | GATC | 3 cut(s) | 403, 489, 1516 |
| Sau96I | GGNCC | 9 cut(s) | 173, 293, 559, 1151, 1201, 1492, 1642, 1672, 1673 |
| ScrFI | CCNGG | 6 cut(s) | 171, 327, 600, 798, 1150, 1590 |
| SduI | GDGCHC | 2 cut(s) | 317, 1676 |
| SexAI | ACCWGGT | 1 cut(s) | 796 |
| SfaNI | GCATC | 4 cut(s) | 66, 739, 1022, 1438 |
| SinI | GGWCC | 6 cut(s) | 173, 293, 559, 1201, 1492, 1642 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 98, 255, 1373, 1434 |
| SmlI | CTYRAG | 1 cut(s) | 1677 |
| SmoI | CTYRAG | 1 cut(s) | 1677 |
| SphI | GCATGC | 1 cut(s) | 389 |
| SsiI | CCGC | 7 cut(s) | 576, 687, 858, 936, 1173, 1277, 1313 |
| SspMI | CTAG | 3 cut(s) | 753, 1176, 1206 |
| StyD4I | CCNGG | 6 cut(s) | 169, 325, 598, 796, 1148, 1588 |
| StyI | CCWWGG | 2 cut(s) | 272, 281 |
| TaiI | ACGT | 1 cut(s) | 1168 |
| TaqI | TCGA | 4 cut(s) | 11, 213, 406, 1515 |
| TaqII | GACCGA | 1 cut(s) | 190 |
| TatI | WGTACW | 2 cut(s) | 415, 1362 |
| TauI | GCSGC | 3 cut(s) | 860, 1175, 1315 |
| TfiI | GAWTC | 5 cut(s) | 277, 319, 623, 1327, 1572 |
| Tru1I | TTAA | 8 cut(s) | 51, 222, 588, 941, 1020, 1080, 1290, 1720 |
| Tru9I | TTAA | 8 cut(s) | 51, 222, 588, 941, 1020, 1080, 1290, 1720 |
| TscAI | CASTG | 6 cut(s) | 257, 317, 400, 418, 829, 1613 |
| TseFI | GTSAC | 2 cut(s) | 730, 784 |
| Tsp45I | GTSAC | 2 cut(s) | 730, 784 |
| TspDTI | ATGAA | 2 cut(s) | 17, 1642 |
| TspGWI | ACGGA | 3 cut(s) | 306, 1102, 1358 |
| TspRI | CASTG | 6 cut(s) | 257, 317, 400, 418, 829, 1613 |
| Tth111I | GACNNNGTC | 1 cut(s) | 203 |
| VpaK11BI | GGWCC | 6 cut(s) | 173, 293, 559, 1201, 1492, 1642 |
| VspI | ATTAAT | 1 cut(s) | 51 |
| XapI | RAATTY | 1 cut(s) | 1250 |
| XceI | RCATGY | 1 cut(s) | 389 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 279 |
| XspI | CTAG | 3 cut(s) | 753, 1176, 1206 |
| Zsp2I | ATGCAT | 2 cut(s) | 1015, 1431 |