Lignin degradation and detoxification of lignin-derived products
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 125 |
| AccII | CGCG | 1 cut(s) | 901 |
| AccIII | TCCGGA | 1 cut(s) | 305 |
| AciI | CCGC | 4 cut(s) | 612, 635, 1013, 1049 |
| AclWI | GGATC | 2 cut(s) | 165, 1247 |
| AcoI | YGGCCR | 1 cut(s) | 609 |
| AcsI | RAATTY | 2 cut(s) | 237, 1329 |
| AcuI | CTGAAG | 2 cut(s) | 1043, 1434 |
| AcyI | GRCGYC | 1 cut(s) | 330 |
| AdeI | CACNNNGTG | 1 cut(s) | 66 |
| AfaI | GTAC | 5 cut(s) | 965, 1043, 1055, 1092, 1100 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 135, 1073, 1289 |
| AflIII | ACRYGT | 2 cut(s) | 855, 899 |
| AgsI | TTSAA | 7 cut(s) | 77, 302, 391, 705, 1237, 1265, 1312 |
| AjnI | CCWGG | 4 cut(s) | 79, 352, 550, 1324 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 985, 1017 |
| AluBI | AGCT | 6 cut(s) | 283, 510, 780, 930, 1117, 1418 |
| AluI | AGCT | 6 cut(s) | 283, 510, 780, 930, 1117, 1418 |
| Alw26I | GTCTC | 3 cut(s) | 377, 421, 776 |
| AlwI | GGATC | 2 cut(s) | 165, 1247 |
| Aor13HI | TCCGGA | 1 cut(s) | 305 |
| AoxI | GGCC | 4 cut(s) | 84, 590, 609, 887 |
| ApeKI | GCWGC | 1 cut(s) | 908 |
| ApoI | RAATTY | 2 cut(s) | 237, 1329 |
| AspLEI | GCGC | 2 cut(s) | 884, 1039 |
| AspS9I | GGNCC | 7 cut(s) | 47, 84, 176, 313, 887, 1228, 1409 |
| AsuC2I | CCSGG | 1 cut(s) | 886 |
| AsuHPI | GGTGA | 4 cut(s) | 85, 532, 540, 1096 |
| AsuNHI | GCTAGC | 1 cut(s) | 506 |
| AvaII | GGWCC | 5 cut(s) | 47, 176, 313, 1228, 1409 |
| BaeGI | GKGCMC | 2 cut(s) | 71, 869 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1082, 1115 |
| BanI | GGYRCC | 1 cut(s) | 125 |
| BanII | GRGCYC | 1 cut(s) | 1300 |
| BbvI | GCAGC | 1 cut(s) | 895 |
| BccI | CCATC | 9 cut(s) | 188, 391, 476, 551, 851, 1164, 1310, 1356, 1369 |
| BciT130I | CCWGG | 4 cut(s) | 81, 354, 552, 1326 |
| BciVI | GTATCC | 2 cut(s) | 375, 1372 |
| BcnI | CCSGG | 1 cut(s) | 886 |
| BcoDI | GTCTC | 3 cut(s) | 377, 421, 776 |
| BfaI | CTAG | 3 cut(s) | 507, 912, 942 |
| BfmI | CTRYAG | 1 cut(s) | 615 |
| BfuI | GTATCC | 2 cut(s) | 375, 1372 |
| BisI | GCNGC | 3 cut(s) | 612, 909, 1049 |
| BlsI | GCNGC | 3 cut(s) | 613, 910, 1050 |
| BmcAI | AGTACT | 1 cut(s) | 1043 |
| Bme1390I | CCNGG | 5 cut(s) | 81, 354, 552, 886, 1326 |
| Bme18I | GGWCC | 5 cut(s) | 47, 176, 313, 1228, 1409 |
| BmgT120I | GGNCC | 7 cut(s) | 47, 84, 176, 313, 887, 1228, 1409 |
| BmiI | GGNNCC | 9 cut(s) | 85, 127, 178, 314, 315, 639, 1230, 1299, 1410 |
| BmrFI | CCNGG | 5 cut(s) | 81, 354, 552, 886, 1326 |
| BmsI | GCATC | 3 cut(s) | 493, 758, 1174 |
| BmtI | GCTAGC | 1 cut(s) | 510 |
| BpmI | CTGGAG | 1 cut(s) | 1115 |
| BpuMI | CCSGG | 1 cut(s) | 886 |
| BsaBI | GATNNNNATC | 2 cut(s) | 363, 1155 |
| BsaHI | GRCGYC | 1 cut(s) | 330 |
| BsaJI | CCNNGG | 3 cut(s) | 35, 80, 172 |
| BsaWI | WCCGGW | 2 cut(s) | 305, 440 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1106, 1136 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 135, 1073, 1289 |
| Bse1I | ACTGG | 2 cut(s) | 862, 1098 |
| Bse3DI | GCAATG | 2 cut(s) | 1272, 1342 |
| Bse8I | GATNNNNATC | 2 cut(s) | 363, 1155 |
| BseAI | TCCGGA | 1 cut(s) | 305 |
| BseBI | CCWGG | 4 cut(s) | 81, 354, 552, 1326 |
| BseDI | CCNNGG | 3 cut(s) | 35, 80, 172 |
| BseGI | GGATG | 3 cut(s) | 1156, 1156, 1380 |
| BseJI | GATNNNNATC | 2 cut(s) | 363, 1155 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 135, 1073, 1289 |
| BseMI | GCAATG | 2 cut(s) | 1272, 1342 |
| BseMII | CTCAG | 3 cut(s) | 305, 416, 787 |
| BseNI | ACTGG | 2 cut(s) | 862, 1098 |
| BseRI | GAGGAG | 1 cut(s) | 1248 |
| BseSI | GKGCMC | 2 cut(s) | 71, 869 |
| BseXI | GCAGC | 1 cut(s) | 895 |
| Bsh1236I | CGCG | 1 cut(s) | 901 |
| BshFI | GGCC | 4 cut(s) | 86, 592, 611, 889 |
| BshNI | GGYRCC | 1 cut(s) | 125 |
| BsiSI | CCGG | 3 cut(s) | 306, 441, 885 |
| BslFI | GGGAC | 8 cut(s) | 162, 326, 511, 910, 1090, 1144, 1265, 1422 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 135, 1073, 1289 |
| BsmAI | GTCTC | 3 cut(s) | 377, 421, 776 |
| BsmBI | CGTCTC | 1 cut(s) | 377 |
| BsmFI | GGGAC | 8 cut(s) | 162, 326, 511, 910, 1090, 1144, 1265, 1422 |
| BsnI | GGCC | 4 cut(s) | 86, 592, 611, 889 |
| Bsp1286I | GDGCHC | 3 cut(s) | 71, 869, 1300 |
| Bsp13I | TCCGGA | 1 cut(s) | 305 |
| Bsp143I | GATC | 4 cut(s) | 102, 157, 358, 1252 |
| Bsp19I | CCATGG | 2 cut(s) | 35, 172 |
| BspACI | CCGC | 4 cut(s) | 612, 635, 1013, 1049 |
| BspANI | GGCC | 4 cut(s) | 86, 592, 611, 889 |
| BspCNI | CTCAG | 3 cut(s) | 304, 417, 786 |
| BspEI | TCCGGA | 1 cut(s) | 305 |
| BspFNI | CGCG | 1 cut(s) | 901 |
| BspLI | GGNNCC | 9 cut(s) | 85, 127, 178, 314, 315, 639, 1230, 1299, 1410 |
| BspOI | GCTAGC | 1 cut(s) | 510 |
| BspPI | GGATC | 2 cut(s) | 165, 1247 |
| BspT107I | GGYRCC | 1 cut(s) | 125 |
| BsrDI | GCAATG | 2 cut(s) | 1272, 1342 |
| BsrI | ACTGG | 2 cut(s) | 862, 1098 |
| BssECI | CCNNGG | 3 cut(s) | 35, 80, 172 |
| BssMI | GATC | 4 cut(s) | 102, 157, 358, 1252 |
| BssNI | GRCGYC | 1 cut(s) | 330 |
| BssT1I | CCWWGG | 2 cut(s) | 35, 172 |
| Bst2UI | CCWGG | 4 cut(s) | 81, 354, 552, 1326 |
| Bst4CI | ACNGT | 6 cut(s) | 66, 149, 169, 490, 1111, 1181 |
| Bst6I | CTCTTC | 1 cut(s) | 1266 |
| BstACI | GRCGYC | 1 cut(s) | 330 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1045 |
| BstC8I | GCNNGC | 3 cut(s) | 8, 141, 508 |
| BstDEI | CTNAG | 3 cut(s) | 291, 425, 773 |
| BstDSI | CCRYGG | 2 cut(s) | 35, 172 |
| BstF5I | GGATG | 3 cut(s) | 1156, 1156, 1380 |
| BstFNI | CGCG | 1 cut(s) | 901 |
| BstHHI | GCGC | 2 cut(s) | 884, 1039 |
| BstKTI | GATC | 4 cut(s) | 105, 160, 361, 1255 |
| BstMAI | GTCTC | 3 cut(s) | 377, 421, 776 |
| BstMBI | GATC | 4 cut(s) | 102, 157, 358, 1252 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 617, 1045 |
| BstNI | CCWGG | 4 cut(s) | 81, 354, 552, 1326 |
| BstNSI | RCATGY | 1 cut(s) | 143 |
| BstSCI | CCNGG | 5 cut(s) | 79, 352, 550, 884, 1324 |
| BstSFI | CTRYAG | 1 cut(s) | 615 |
| BstSLI | GKGCMC | 2 cut(s) | 71, 869 |
| BstUI | CGCG | 1 cut(s) | 901 |
| BstV1I | GCAGC | 1 cut(s) | 895 |
| BsuI | GTATCC | 2 cut(s) | 375, 1372 |
| BsuRI | GGCC | 4 cut(s) | 86, 592, 611, 889 |
| BtgI | CCRYGG | 2 cut(s) | 35, 172 |
| BtgZI | GCGATG | 1 cut(s) | 454 |
| BtsCI | GGATG | 3 cut(s) | 1156, 1156, 1380 |
| BtsIMutI | CAGTG | 4 cut(s) | 71, 154, 869, 1116 |
| Cac8I | GCNNGC | 3 cut(s) | 8, 141, 508 |
| CfoI | GCGC | 2 cut(s) | 884, 1039 |
| Cfr13I | GGNCC | 7 cut(s) | 47, 84, 176, 313, 887, 1228, 1409 |
| CseI | GACGC | 3 cut(s) | 338, 890, 907 |
| CsiI | ACCWGGT | 1 cut(s) | 550 |
| Csp6I | GTAC | 5 cut(s) | 964, 1042, 1054, 1091, 1099 |
| CviQI | GTAC | 5 cut(s) | 964, 1042, 1054, 1091, 1099 |
| DdeI | CTNAG | 3 cut(s) | 291, 425, 773 |
| DpnI | GATC | 4 cut(s) | 104, 159, 360, 1254 |
| DpnII | GATC | 4 cut(s) | 102, 157, 358, 1252 |
| DraIII | CACNNNGTG | 1 cut(s) | 66 |
| EaeI | YGGCCR | 1 cut(s) | 609 |
| Eam1104I | CTCTTC | 1 cut(s) | 1266 |
| EarI | CTCTTC | 1 cut(s) | 1266 |
| Eco130I | CCWWGG | 2 cut(s) | 35, 172 |
| Eco147I | AGGCCT | 1 cut(s) | 592 |
| Eco24I | GRGCYC | 1 cut(s) | 1300 |
| Eco32I | GATATC | 1 cut(s) | 32 |
| Eco47I | GGWCC | 5 cut(s) | 47, 176, 313, 1228, 1409 |
| Eco57I | CTGAAG | 2 cut(s) | 1043, 1434 |
| EcoO109I | RGGNCCY | 3 cut(s) | 313, 1228, 1409 |
| EcoRII | CCWGG | 4 cut(s) | 79, 352, 550, 1324 |
| EcoRV | GATATC | 1 cut(s) | 32 |
| EcoT14I | CCWWGG | 2 cut(s) | 35, 172 |
| EcoT22I | ATGCAT | 3 cut(s) | 751, 1167, 1344 |
| EcoT38I | GRGCYC | 1 cut(s) | 1300 |
| ErhI | CCWWGG | 2 cut(s) | 35, 172 |
| Esp3I | CGTCTC | 1 cut(s) | 377 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 114, 146 |
| FaqI | GGGAC | 8 cut(s) | 162, 326, 511, 910, 1090, 1144, 1265, 1422 |
| FauI | CCCGC | 1 cut(s) | 1020 |
| FauNDI | CATATG | 1 cut(s) | 1362 |
| Fnu4HI | GCNGC | 3 cut(s) | 612, 909, 1049 |
| FokI | GGATG | 3 cut(s) | 1143, 1163, 1387 |
| FriOI | GRGCYC | 1 cut(s) | 1300 |
| Fsp4HI | GCNGC | 3 cut(s) | 612, 909, 1049 |
| FspBI | CTAG | 3 cut(s) | 507, 912, 942 |
| GlaI | GCGC | 2 cut(s) | 883, 1038 |
| GluI | GCNGC | 3 cut(s) | 612, 909, 1049 |
| GsuI | CTGGAG | 1 cut(s) | 1115 |
| HaeIII | GGCC | 4 cut(s) | 86, 592, 611, 889 |
| HapII | CCGG | 3 cut(s) | 306, 441, 885 |
| HgaI | GACGC | 3 cut(s) | 338, 890, 907 |
| HhaI | GCGC | 2 cut(s) | 884, 1039 |
| Hin1I | GRCGYC | 1 cut(s) | 330 |
| Hin6I | GCGC | 2 cut(s) | 882, 1037 |
| HinP1I | GCGC | 2 cut(s) | 882, 1037 |
| HincII | GTYRAC | 3 cut(s) | 350, 853, 979 |
| HindII | GTYRAC | 3 cut(s) | 350, 853, 979 |
| HindIII | AAGCTT | 1 cut(s) | 281 |
| HinfI | GANTC | 3 cut(s) | 454, 682, 1308 |
| HpaI | GTTAAC | 1 cut(s) | 853 |
| HpaII | CCGG | 3 cut(s) | 306, 441, 885 |
| HphI | GGTGA | 4 cut(s) | 85, 532, 540, 1096 |
| Hpy166II | GTNNAC | 5 cut(s) | 93, 350, 853, 937, 979 |
| Hpy188I | TCNGA | 7 cut(s) | 43, 294, 328, 382, 588, 1062, 1307 |
| Hpy188III | TCNNGA | 4 cut(s) | 112, 306, 775, 1352 |
| Hpy8I | GTNNAC | 5 cut(s) | 93, 350, 853, 937, 979 |
| Hpy99I | CGWCG | 1 cut(s) | 332 |
| HpyAV | CCTTC | 6 cut(s) | 109, 116, 259, 708, 1067, 1081 |
| HpyCH4III | ACNGT | 6 cut(s) | 66, 149, 169, 490, 1111, 1181 |
| HpyCH4IV | ACGT | 4 cut(s) | 386, 624, 807, 857 |
| HpyCH4V | TGCA | 5 cut(s) | 233, 448, 749, 1165, 1342 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 617, 1045 |
| HpyF3I | CTNAG | 3 cut(s) | 291, 425, 773 |
| HpySE526I | ACGT | 4 cut(s) | 386, 624, 807, 857 |
| Hsp92I | GRCGYC | 1 cut(s) | 330 |
| HspAI | GCGC | 2 cut(s) | 882, 1037 |
| KflI | GGGWCCC | 1 cut(s) | 313 |
| Kpn2I | TCCGGA | 1 cut(s) | 305 |
| KspAI | GTTAAC | 1 cut(s) | 853 |
| Kzo9I | GATC | 4 cut(s) | 102, 157, 358, 1252 |
| LmnI | GCTCC | 4 cut(s) | 579, 777, 1114, 1303 |
| Lsp1109I | GCAGC | 1 cut(s) | 895 |
| LweI | GCATC | 3 cut(s) | 493, 758, 1174 |
| MabI | ACCWGGT | 1 cut(s) | 550 |
| MaeI | CTAG | 3 cut(s) | 507, 912, 942 |
| MaeII | ACGT | 4 cut(s) | 386, 624, 807, 857 |
| MaeIII | GTNAC | 4 cut(s) | 484, 538, 689, 1175 |
| MalI | GATC | 4 cut(s) | 104, 159, 360, 1254 |
| MboI | GATC | 4 cut(s) | 102, 157, 358, 1252 |
| MboII | GAAGA | 4 cut(s) | 693, 716, 1160, 1253 |
| MhlI | GDGCHC | 3 cut(s) | 71, 869, 1300 |
| MluCI | AATT | 7 cut(s) | 72, 237, 819, 874, 986, 1135, 1329 |
| MluI | ACGCGT | 1 cut(s) | 899 |
| MmeI | TCCRAC | 3 cut(s) | 21, 351, 1174 |
| Mph1103I | ATGCAT | 3 cut(s) | 751, 1167, 1344 |
| MroI | TCCGGA | 1 cut(s) | 305 |
| MseI | TTAA | 1 cut(s) | 852 |
| MslI | CAYNNNNRTG | 2 cut(s) | 762, 1170 |
| MspA1I | CMGCKG | 1 cut(s) | 1051 |
| MspI | CCGG | 3 cut(s) | 306, 441, 885 |
| MspR9I | CCNGG | 5 cut(s) | 81, 354, 552, 886, 1326 |
| MvaI | CCWGG | 4 cut(s) | 81, 354, 552, 1326 |
| MvnI | CGCG | 1 cut(s) | 901 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 617, 1045 |
| NciI | CCSGG | 1 cut(s) | 886 |
| NcoI | CCATGG | 2 cut(s) | 35, 172 |
| NdeI | CATATG | 1 cut(s) | 1362 |
| NdeII | GATC | 4 cut(s) | 102, 157, 358, 1252 |
| NheI | GCTAGC | 1 cut(s) | 506 |
| NlaIV | GGNNCC | 9 cut(s) | 85, 127, 178, 314, 315, 639, 1230, 1299, 1410 |
| NmuCI | GTSAC | 1 cut(s) | 538 |
| NsiI | ATGCAT | 3 cut(s) | 751, 1167, 1344 |
| NspI | RCATGY | 1 cut(s) | 143 |
| PaeI | GCATGC | 1 cut(s) | 143 |
| PceI | AGGCCT | 1 cut(s) | 592 |
| PfeI | GAWTC | 3 cut(s) | 454, 682, 1308 |
| PfoI | TCCNGGA | 1 cut(s) | 1324 |
| PkrI | GCNGC | 3 cut(s) | 613, 910, 1050 |
| PpuMI | RGGWCCY | 3 cut(s) | 313, 1228, 1409 |
| Psp5II | RGGWCCY | 3 cut(s) | 313, 1228, 1409 |
| Psp6I | CCWGG | 4 cut(s) | 79, 352, 550, 1324 |
| PspGI | CCWGG | 4 cut(s) | 79, 352, 550, 1324 |
| PspN4I | GGNNCC | 9 cut(s) | 85, 127, 178, 314, 315, 639, 1230, 1299, 1410 |
| PspPI | GGNCC | 7 cut(s) | 47, 84, 176, 313, 887, 1228, 1409 |
| PspPPI | RGGWCCY | 3 cut(s) | 313, 1228, 1409 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 194, 226 |
| RsaI | GTAC | 5 cut(s) | 965, 1043, 1055, 1092, 1100 |
| RsaNI | GTAC | 5 cut(s) | 964, 1042, 1054, 1091, 1099 |
| RseI | CAYNNNNRTG | 2 cut(s) | 762, 1170 |
| SaqAI | TTAA | 1 cut(s) | 852 |
| SatI | GCNGC | 3 cut(s) | 612, 909, 1049 |
| Sau3AI | GATC | 4 cut(s) | 102, 157, 358, 1252 |
| Sau96I | GGNCC | 7 cut(s) | 47, 84, 176, 313, 887, 1228, 1409 |
| ScaI | AGTACT | 1 cut(s) | 1043 |
| ScrFI | CCNGG | 5 cut(s) | 81, 354, 552, 886, 1326 |
| SduI | GDGCHC | 3 cut(s) | 71, 869, 1300 |
| SexAI | ACCWGGT | 1 cut(s) | 550 |
| SfaNI | GCATC | 3 cut(s) | 493, 758, 1174 |
| SfcI | CTRYAG | 1 cut(s) | 615 |
| SinI | GGWCC | 5 cut(s) | 47, 176, 313, 1228, 1409 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 762, 1170 |
| SphI | GCATGC | 1 cut(s) | 143 |
| Sse9I | AATT | 7 cut(s) | 72, 237, 819, 874, 986, 1135, 1329 |
| SseBI | AGGCCT | 1 cut(s) | 592 |
| SsiI | CCGC | 4 cut(s) | 612, 635, 1013, 1049 |
| SspMI | CTAG | 3 cut(s) | 507, 912, 942 |
| StuI | AGGCCT | 1 cut(s) | 592 |
| StyD4I | CCNGG | 5 cut(s) | 79, 352, 550, 884, 1324 |
| StyI | CCWWGG | 2 cut(s) | 35, 172 |
| TaaI | ACNGT | 6 cut(s) | 66, 149, 169, 490, 1111, 1181 |
| TaiI | ACGT | 4 cut(s) | 389, 627, 810, 860 |
| TaqI | TCGA | 1 cut(s) | 113 |
| TasI | AATT | 7 cut(s) | 72, 237, 819, 874, 986, 1135, 1329 |
| TatI | WGTACW | 2 cut(s) | 1041, 1098 |
| TauI | GCSGC | 2 cut(s) | 614, 1051 |
| TfiI | GAWTC | 3 cut(s) | 454, 682, 1308 |
| Tru1I | TTAA | 1 cut(s) | 852 |
| Tru9I | TTAA | 1 cut(s) | 852 |
| TscAI | CASTG | 4 cut(s) | 71, 154, 869, 1116 |
| TseFI | GTSAC | 1 cut(s) | 538 |
| TseI | GCWGC | 1 cut(s) | 908 |
| Tsp45I | GTSAC | 1 cut(s) | 538 |
| TspDTI | ATGAA | 4 cut(s) | 746, 867, 1160, 1378 |
| TspGWI | ACGGA | 2 cut(s) | 60, 838 |
| TspRI | CASTG | 4 cut(s) | 71, 154, 869, 1116 |
| VpaK11BI | GGWCC | 5 cut(s) | 47, 176, 313, 1228, 1409 |
| XapI | RAATTY | 2 cut(s) | 237, 1329 |
| XceI | RCATGY | 1 cut(s) | 143 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 33 |
| XspI | CTAG | 3 cut(s) | 507, 912, 942 |
| ZrmI | AGTACT | 1 cut(s) | 1043 |
| Zsp2I | ATGCAT | 3 cut(s) | 751, 1167, 1344 |