Prupe.2G143100_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000574
Physical Location & Seq
Reverse (-)
20017041 .. 20018243
1203 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G143100.1

Sequence Viewer

Length: 585 bp
ATGCATGCGGAAATCCAATCGTATTTTAATGAAGTTATTTACACCATCACCCCTGAAGTTTTTCACCTTTACACAAAATCCCCTGATATTTTAGAAATTACAAGAACATCCCCTGAGGTGATGAACAAATCTTCGGCCAGAAATTCTAACCATCATGATCATGACATTCTTGCTTTCATCATTCCCATACTGCTTACCTTTGTCCAGATCAGATATCTAGCTGGTCATCAAGATCTGTTCCAAACACACCCATTGACCATGATGGTTATGCTGTCTAGCTTACTTCCATACTGCTTTGCCTTCAGCTTACTTATAAGGCTTAGTGGTAGTGGTAGTCGTATGTATAATTGGTGCCGCCTGGCTATGATGGTGTTTGGTTTTCTCTCAGTTGCTTCACTCTTGTGTCTTCTGTTTATTCCAAACTACTCCAATCTAACCTTGTGCATCTTCTTCTTGTTGTTGTTGGTGGCGTTGGGCTTGAACCGCTTAGTCCGGAGATTGTGGCAGCACATCCGAACGGTACAAAATCGCGGCAGGACATGCCCTCTTTTGCCGTCGACCTTAATGGATATTGAACAAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

195

Amino Acids

22.37

Weight (kDa)

8.68

Isoelectric Point (pI)

45.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 314
AccB1I GGYRCC 1 cut(s) 351
AccI GTMKAC 1 cut(s) 557
AccII CGCG 1 cut(s) 531
AccIII TCCGGA 1 cut(s) 492
AciI CCGC 4 cut(s) 8, 355, 484, 531
AcoI YGGCCR 1 cut(s) 135
AcsI RAATTY 1 cut(s) 142
AcuI CTGAAG 2 cut(s) 75, 286
AfaI GTAC 1 cut(s) 522
AgsI TTSAA 2 cut(s) 481, 575
AjnI CCWGG 1 cut(s) 357
AleI CACNNNNGTG 1 cut(s) 400
AluBI AGCT 3 cut(s) 221, 279, 306
AluI AGCT 3 cut(s) 221, 279, 306
Aor13HI TCCGGA 1 cut(s) 492
AoxI GGCC 1 cut(s) 135
ApeKI GCWGC 1 cut(s) 505
ApoI RAATTY 1 cut(s) 142
Asp700I GAANNNNTTC 1 cut(s) 60
AsuHPI GGTGA 3 cut(s) 40, 56, 130
AxyI CCTNAGG 1 cut(s) 114
BanI GGYRCC 1 cut(s) 351
BbsI GAAGAC 1 cut(s) 398
BbvI GCAGC 1 cut(s) 517
BccI CCATC 4 cut(s) 53, 159, 256, 361
BceAI ACGGC 1 cut(s) 538
BciT130I CCWGG 1 cut(s) 359
BclI TGATCA 1 cut(s) 157
BfaI CTAG 2 cut(s) 218, 276
BglII AGATCT 1 cut(s) 232
BisI GCNGC 3 cut(s) 355, 506, 532
BlsI GCNGC 3 cut(s) 356, 507, 533
Bme1390I CCNGG 1 cut(s) 359
BmiI GGNNCC 1 cut(s) 353
BmrFI CCNGG 1 cut(s) 359
BmsI GCATC 1 cut(s) 453
BpiI GAAGAC 1 cut(s) 398
BsaWI WCCGGW 1 cut(s) 492
Bse21I CCTNAGG 1 cut(s) 114
BseAI TCCGGA 1 cut(s) 492
BseBI CCWGG 1 cut(s) 359
BseGI GGATG 2 cut(s) 107, 510
BseMII CTCAG 2 cut(s) 105, 399
BseXI GCAGC 1 cut(s) 517
Bsh1236I CGCG 1 cut(s) 531
BshFI GGCC 1 cut(s) 137
BshNI GGYRCC 1 cut(s) 351
BsiSI CCGG 1 cut(s) 493
BsnI GGCC 1 cut(s) 137
Bsp13I TCCGGA 1 cut(s) 492
Bsp143I GATC 3 cut(s) 157, 207, 232
BspACI CCGC 4 cut(s) 8, 355, 484, 531
BspANI GGCC 1 cut(s) 137
BspCNI CTCAG 2 cut(s) 106, 398
BspEI TCCGGA 1 cut(s) 492
BspFNI CGCG 1 cut(s) 531
BspHI TCATGA 2 cut(s) 154, 160
BspLI GGNNCC 1 cut(s) 353
BspT107I GGYRCC 1 cut(s) 351
BssMI GATC 3 cut(s) 157, 207, 232
Bst2UI CCWGG 1 cut(s) 359
Bst4CI ACNGT 1 cut(s) 520
BstAPI GCANNNNNTGC 1 cut(s) 540
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 4 cut(s) 114, 320, 385, 487
BstF5I GGATG 2 cut(s) 107, 510
BstFNI CGCG 1 cut(s) 531
BstKTI GATC 3 cut(s) 160, 210, 235
BstMBI GATC 3 cut(s) 157, 207, 232
BstMWI GCNNNNNNNGC 2 cut(s) 483, 540
BstNI CCWGG 1 cut(s) 359
BstNSI RCATGY 2 cut(s) 8, 543
BstSCI CCNGG 1 cut(s) 357
BstUI CGCG 1 cut(s) 531
BstV1I GCAGC 1 cut(s) 517
BstV2I GAAGAC 1 cut(s) 398
BstX2I RGATCY 1 cut(s) 232
BstYI RGATCY 1 cut(s) 232
Bsu36I CCTNAGG 1 cut(s) 114
BsuRI GGCC 1 cut(s) 137
BtsCI GGATG 2 cut(s) 107, 510
Cac8I GCNNGC 1 cut(s) 6
CciI TCATGA 2 cut(s) 154, 160
Csp6I GTAC 1 cut(s) 521
CviAII CATG 5 cut(s) 5, 155, 161, 259, 540
CviJI RGCY 7 cut(s) 137, 221, 279, 306, 319, 362, 477
CviKI_1 RGCY 7 cut(s) 137, 221, 279, 306, 319, 362, 477
CviQI GTAC 1 cut(s) 521
DdeI CTNAG 4 cut(s) 114, 320, 385, 487
DpnI GATC 3 cut(s) 159, 209, 234
DpnII GATC 3 cut(s) 157, 207, 232
EaeI YGGCCR 1 cut(s) 135
Eco32I GATATC 1 cut(s) 215
Eco57I CTGAAG 2 cut(s) 75, 286
Eco81I CCTNAGG 1 cut(s) 114
EcoRII CCWGG 1 cut(s) 357
EcoRV GATATC 1 cut(s) 215
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 5 cut(s) 8, 158, 164, 262, 543
FatI CATG 5 cut(s) 4, 154, 160, 258, 539
FbaI TGATCA 1 cut(s) 157
FblI GTMKAC 1 cut(s) 557
Fnu4HI GCNGC 3 cut(s) 355, 506, 532
FokI GGATG 2 cut(s) 94, 497
Fsp4HI GCNGC 3 cut(s) 355, 506, 532
FspBI CTAG 2 cut(s) 218, 276
GluI GCNGC 3 cut(s) 355, 506, 532
HaeIII GGCC 1 cut(s) 137
HapII CCGG 1 cut(s) 493
Hin1II CATG 5 cut(s) 8, 158, 164, 262, 543
HincII GTYRAC 1 cut(s) 558
HindII GTYRAC 1 cut(s) 558
HpaII CCGG 1 cut(s) 493
HphI GGTGA 3 cut(s) 40, 56, 130
Hpy166II GTNNAC 1 cut(s) 558
Hpy188I TCNGA 2 cut(s) 212, 515
Hpy188III TCNNGA 5 cut(s) 155, 161, 205, 230, 493
Hpy8I GTNNAC 1 cut(s) 558
Hpy99I CGWCG 1 cut(s) 559
HpyAV CCTTC 1 cut(s) 310
HpyCH4III ACNGT 1 cut(s) 520
HpyCH4V TGCA 2 cut(s) 4, 444
HpyF10VI GCNNNNNNNGC 2 cut(s) 483, 540
HpyF3I CTNAG 4 cut(s) 114, 320, 385, 487
Hsp92II CATG 5 cut(s) 8, 158, 164, 262, 543
Kpn2I TCCGGA 1 cut(s) 492
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 3 cut(s) 157, 207, 232
Lsp1109I GCAGC 1 cut(s) 517
LweI GCATC 1 cut(s) 453
MaeI CTAG 2 cut(s) 218, 276
MalI GATC 3 cut(s) 159, 209, 234
MboI GATC 3 cut(s) 157, 207, 232
MboII GAAGA 4 cut(s) 123, 398, 439, 442
MflI RGATCY 1 cut(s) 232
MluCI AATT 3 cut(s) 96, 142, 346
MnlI CCTC 2 cut(s) 109, 555
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 492
MroXI GAANNNNTTC 1 cut(s) 60
MseI TTAA 2 cut(s) 27, 563
MslI CAYNNNNRTG 2 cut(s) 159, 400
MspI CCGG 1 cut(s) 493
MspR9I CCNGG 1 cut(s) 359
MvaI CCWGG 1 cut(s) 359
MvnI CGCG 1 cut(s) 531
MwoI GCNNNNNNNGC 2 cut(s) 483, 540
NdeII GATC 3 cut(s) 157, 207, 232
NlaIII CATG 5 cut(s) 8, 158, 164, 262, 543
NlaIV GGNNCC 1 cut(s) 353
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 8, 543
OliI CACNNNNGTG 1 cut(s) 400
PaeI GCATGC 1 cut(s) 8
PagI TCATGA 2 cut(s) 154, 160
PdmI GAANNNNTTC 1 cut(s) 60
PkrI GCNGC 3 cut(s) 356, 507, 533
PsiI TTATAA 1 cut(s) 314
Psp6I CCWGG 1 cut(s) 357
PspGI CCWGG 1 cut(s) 357
PspN4I GGNNCC 1 cut(s) 353
PsuI RGATCY 1 cut(s) 232
RsaI GTAC 1 cut(s) 522
RsaNI GTAC 1 cut(s) 521
RseI CAYNNNNRTG 2 cut(s) 159, 400
SalI GTCGAC 1 cut(s) 556
SaqAI TTAA 2 cut(s) 27, 563
SatI GCNGC 3 cut(s) 355, 506, 532
Sau3AI GATC 3 cut(s) 157, 207, 232
ScrFI CCNGG 1 cut(s) 359
SetI ASST 8 cut(s) 69, 120, 200, 223, 281, 308, 440, 563
SfaNI GCATC 1 cut(s) 453
SmiMI CAYNNNNRTG 2 cut(s) 159, 400
SphI GCATGC 1 cut(s) 8
Sse9I AATT 3 cut(s) 96, 142, 346
SsiI CCGC 4 cut(s) 8, 355, 484, 531
SspMI CTAG 2 cut(s) 218, 276
StyD4I CCNGG 1 cut(s) 357
TaaI ACNGT 1 cut(s) 520
TaqI TCGA 1 cut(s) 557
TasI AATT 3 cut(s) 96, 142, 346
TauI GCSGC 2 cut(s) 357, 534
Tru1I TTAA 2 cut(s) 27, 563
Tru9I TTAA 2 cut(s) 27, 563
TseI GCWGC 1 cut(s) 505
TspDTI ATGAA 3 cut(s) 45, 137, 166
XapI RAATTY 1 cut(s) 142
XceI RCATGY 2 cut(s) 8, 543
XmiI GTMKAC 1 cut(s) 557
XmnI GAANNNNTTC 1 cut(s) 60
XspI CTAG 2 cut(s) 218, 276
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.