pycom07g10060

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000574
Physical Location & Seq
Reverse (-)
9665423 .. 9667212
1790 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g10060.1

Sequence Viewer

Length: 591 bp
ATGGAGAGCAGAGAGCACCATGCTGACTTTGCGGTTTGGAGATCTAGCAGCAGCAGCAATAAAGTCTCCAAATGGAATCATATCAAGCTCAGAAGGAAAATAAAAAATAAGAGGTTGCAAGGGTTCATTTCCTCAGTGCCATGGCATGCCATCTTTGCATTTCTGAATCCACTGCTAATCGGCGTCCTTCAAGTGAAATGTCAAGGTGCAAACGTCTCCCCATTCGACACACACCCAAGAGTCATGTGGATATTTTTGTCGACAACAGTAGTTTACTGCTTTGCATTTGCAGCTAACATAAAATCACGCCGTAACTGCACTACCATATTCTACTCTCGAATGTCCGGTCATATTGCTCTACTCACCGGCTCTCTTTCTTCAGTCTCTTTAGTCTCCATCTTCTTTCCAAGCGTGCTTGATGAGCGACTTGTTTTCTTCTCTTGGATCATTATGCCTTTGATTGTTGGAAGAACGTTGATTCGTCGAGTGTGTCAAAAGATTTGCAAATCTACGATGAATGCAGTCATCATAGTTTATAGCATATGGCAGGGGTTTGCAAGAAGGATGGAGCAGCCACAATTGCCGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

22.54

Weight (kDa)

10.79

Isoelectric Point (pI)

59.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 260
AciI CCGC 1 cut(s) 32
AclI AACGTT 1 cut(s) 473
AclWI GGATC 1 cut(s) 452
AcuI CTGAAG 1 cut(s) 363
AcyI GRCGYC 1 cut(s) 183
AgsI TTSAA 1 cut(s) 191
AluBI AGCT 2 cut(s) 88, 293
AluI AGCT 2 cut(s) 88, 293
Alw21I GWGCWC 1 cut(s) 18
Alw26I GTCTC 4 cut(s) 70, 220, 388, 397
AlwI GGATC 1 cut(s) 452
ApeKI GCWGC 5 cut(s) 48, 51, 54, 290, 571
ArsI GACNNNNNNTTYG 2 cut(s) 331, 363
AsuHPI GGTGA 1 cut(s) 355
Bbv12I GWGCWC 1 cut(s) 18
BbvI GCAGC 5 cut(s) 60, 63, 66, 302, 583
BccI CCATC 3 cut(s) 158, 404, 559
BceAI ACGGC 1 cut(s) 294
BcoDI GTCTC 4 cut(s) 70, 220, 388, 397
BfaI CTAG 1 cut(s) 45
BglII AGATCT 1 cut(s) 41
BisI GCNGC 5 cut(s) 49, 52, 55, 291, 572
BlsI GCNGC 5 cut(s) 50, 53, 56, 292, 573
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 140
BsaWI WCCGGW 1 cut(s) 344
Bse118I RCCGGY 1 cut(s) 365
BseDI CCNNGG 1 cut(s) 140
BseGI GGATG 1 cut(s) 570
BseMII CTCAG 2 cut(s) 103, 147
BseXI GCAGC 5 cut(s) 60, 63, 66, 302, 583
BsgI GTGCAG 1 cut(s) 301
BsiHKAI GWGCWC 1 cut(s) 18
BsiSI CCGG 2 cut(s) 345, 366
BsmAI GTCTC 4 cut(s) 70, 220, 388, 397
BsmBI CGTCTC 1 cut(s) 220
BsmI GAATGC 1 cut(s) 523
Bsp1286I GDGCHC 1 cut(s) 18
Bsp143I GATC 2 cut(s) 41, 444
Bsp19I CCATGG 1 cut(s) 140
BspACI CCGC 1 cut(s) 32
BspCNI CTCAG 2 cut(s) 102, 146
BspPI GGATC 1 cut(s) 452
BsrFI RCCGGY 1 cut(s) 365
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 1 cut(s) 140
BssMI GATC 2 cut(s) 41, 444
BssNI GRCGYC 1 cut(s) 183
BssT1I CCWWGG 1 cut(s) 140
Bst4CI ACNGT 1 cut(s) 268
BstACI GRCGYC 1 cut(s) 183
BstC8I GCNNGC 2 cut(s) 147, 413
BstDEI CTNAG 2 cut(s) 89, 133
BstDSI CCRYGG 1 cut(s) 140
BstF5I GGATG 1 cut(s) 570
BstKTI GATC 2 cut(s) 44, 447
BstMAI GTCTC 4 cut(s) 70, 220, 388, 397
BstMBI GATC 2 cut(s) 41, 444
BstMWI GCNNNNNNNGC 7 cut(s) 29, 54, 155, 290, 315, 421, 580
BstNSI RCATGY 1 cut(s) 149
BstV1I GCAGC 5 cut(s) 60, 63, 66, 302, 583
BstX2I RGATCY 1 cut(s) 41
BstYI RGATCY 1 cut(s) 41
BtgI CCRYGG 1 cut(s) 140
BtsCI GGATG 1 cut(s) 570
BtsI GCAGTG 1 cut(s) 170
BtsIMutI CAGTG 2 cut(s) 141, 170
Cac8I GCNNGC 2 cut(s) 147, 413
Cfr10I RCCGGY 1 cut(s) 365
CseI GACGC 1 cut(s) 172
CviAII CATG 4 cut(s) 20, 141, 146, 244
CviJI RGCY 4 cut(s) 88, 293, 369, 574
CviKI_1 RGCY 4 cut(s) 88, 293, 369, 574
DdeI CTNAG 2 cut(s) 89, 133
DpnI GATC 2 cut(s) 43, 446
DpnII GATC 2 cut(s) 41, 444
Eco130I CCWWGG 1 cut(s) 140
Eco57I CTGAAG 1 cut(s) 363
EcoT14I CCWWGG 1 cut(s) 140
ErhI CCWWGG 1 cut(s) 140
Esp3I CGTCTC 1 cut(s) 220
FaeI CATG 4 cut(s) 23, 144, 149, 247
FatI CATG 4 cut(s) 19, 140, 145, 243
FauNDI CATATG 1 cut(s) 542
FblI GTMKAC 1 cut(s) 260
Fnu4HI GCNGC 5 cut(s) 49, 52, 55, 291, 572
FokI GGATG 1 cut(s) 577
Fsp4HI GCNGC 5 cut(s) 49, 52, 55, 291, 572
FspBI CTAG 1 cut(s) 45
GluI GCNGC 5 cut(s) 49, 52, 55, 291, 572
HapII CCGG 2 cut(s) 345, 366
HgaI GACGC 1 cut(s) 172
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 4 cut(s) 23, 144, 149, 247
HincII GTYRAC 1 cut(s) 261
HindII GTYRAC 1 cut(s) 261
HinfI GANTC 4 cut(s) 76, 166, 240, 478
HpaII CCGG 2 cut(s) 345, 366
HphI GGTGA 1 cut(s) 355
Hpy166II GTNNAC 2 cut(s) 261, 274
Hpy188I TCNGA 2 cut(s) 92, 165
Hpy188III TCNNGA 1 cut(s) 336
Hpy8I GTNNAC 2 cut(s) 261, 274
Hpy99I CGWCG 1 cut(s) 486
HpyAV CCTTC 3 cut(s) 87, 197, 555
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4IV ACGT 2 cut(s) 213, 473
HpyCH4V TGCA 9 cut(s) 118, 158, 209, 284, 290, 318, 504, 521, 557
HpyF10VI GCNNNNNNNGC 7 cut(s) 29, 54, 155, 290, 315, 421, 580
HpyF3I CTNAG 2 cut(s) 89, 133
HpySE526I ACGT 2 cut(s) 213, 473
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 4 cut(s) 23, 144, 149, 247
Kzo9I GATC 2 cut(s) 41, 444
LmnI GCTCC 1 cut(s) 568
LpnPI CCDG 3 cut(s) 358, 379, 533
Lsp1109I GCAGC 5 cut(s) 60, 63, 66, 302, 583
MaeI CTAG 1 cut(s) 45
MaeII ACGT 2 cut(s) 213, 473
MaeIII GTNAC 1 cut(s) 311
MalI GATC 2 cut(s) 43, 446
MboI GATC 2 cut(s) 41, 444
MboII GAAGA 4 cut(s) 369, 391, 427, 480
MfeI CAATTG 1 cut(s) 578
MflI RGATCY 1 cut(s) 41
MhlI GDGCHC 1 cut(s) 18
MluCI AATT 1 cut(s) 578
MlyI GAGTC 1 cut(s) 249
MmeI TCCRAC 1 cut(s) 445
MnlI CCTC 2 cut(s) 105, 142
MspI CCGG 2 cut(s) 345, 366
MunI CAATTG 1 cut(s) 578
Mva1269I GAATGC 1 cut(s) 523
MwoI GCNNNNNNNGC 7 cut(s) 29, 54, 155, 290, 315, 421, 580
NcoI CCATGG 1 cut(s) 140
NdeI CATATG 1 cut(s) 542
NdeII GATC 2 cut(s) 41, 444
NlaIII CATG 4 cut(s) 23, 144, 149, 247
NspI RCATGY 1 cut(s) 149
PaeI GCATGC 1 cut(s) 149
PctI GAATGC 1 cut(s) 523
PfeI GAWTC 3 cut(s) 76, 166, 478
PkrI GCNGC 5 cut(s) 50, 53, 56, 292, 573
PleI GAGTC 1 cut(s) 248
PpsI GAGTC 1 cut(s) 248
Psp1406I AACGTT 1 cut(s) 473
PsuI RGATCY 1 cut(s) 41
SalI GTCGAC 1 cut(s) 259
SatI GCNGC 5 cut(s) 49, 52, 55, 291, 572
Sau3AI GATC 2 cut(s) 41, 444
SchI GAGTC 1 cut(s) 249
SduI GDGCHC 1 cut(s) 18
SetI ASST 6 cut(s) 90, 116, 208, 216, 295, 476
SphI GCATGC 1 cut(s) 149
Sse9I AATT 1 cut(s) 578
SsiI CCGC 1 cut(s) 32
SspMI CTAG 1 cut(s) 45
StyI CCWWGG 1 cut(s) 140
TaaI ACNGT 1 cut(s) 268
TaiI ACGT 2 cut(s) 216, 476
TaqI TCGA 4 cut(s) 225, 260, 337, 484
TasI AATT 1 cut(s) 578
TfiI GAWTC 3 cut(s) 76, 166, 478
TscAI CASTG 2 cut(s) 141, 177
TseI GCWGC 5 cut(s) 48, 51, 54, 290, 571
TspDTI ATGAA 2 cut(s) 115, 530
TspRI CASTG 2 cut(s) 141, 177
XceI RCATGY 1 cut(s) 149
XcmI CCANNNNNNNNNTGG 1 cut(s) 243
XmiI GTMKAC 1 cut(s) 260
XspI CTAG 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.