Rh2BG150000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000574
Physical Location & Seq
Forward (+)
12827962 .. 12828486
525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG150000.1

Sequence Viewer

Length: 525 bp
ATGGCCGATTTTCAATATCAAGGGCCACTCTTACGACGTGAATTTTATAATATCGTCATCTTCAACGTTGTACAAGCGGCCCCTTCCACCTTCGCCATTTTCACTTTCATAGTGCCAATACTTCTCACCTTCATCCAGATCAAATTCCCGGCTGTCGAAACTCTACCTCTCCCATTTGAAACCCACCCCATCAAGTTCATGGCTGCCATTCTCAGCTTACTTGCCTTCTGTTGCTTTGCCATTGGAGCCACCCAAAACTTCTTCCCAAATCACGCTCCAAAGCTTTCAATGGCTATCAAGTATACGGCCTCCCTCTCCGTGGCTTCACTGGTGTCGCTTTTGCTTCCCCAGCCTTGGGACCAAGTGCCGTACGTGACCTACTTCTTGTATACATGCCACTGTCTCGGCTTACTCAGAAATATTAATCTGGCTTATTACTACGACATCGCTTGTGGATTTGTGCGTAGCTCACGCGCCCGTCGACAGCACTTGAGACAAACGCGCCGCCCACCGTTGCCGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

174

Amino Acids

19.87

Weight (kDa)

9.51

Isoelectric Point (pI)

64.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 48
AccI GTMKAC 3 cut(s) 302, 389, 481
AccII CGCG 2 cut(s) 474, 502
AciI CCGC 2 cut(s) 77, 505
AclI AACGTT 1 cut(s) 66
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 41, 143
AfaI GTAC 2 cut(s) 72, 371
AfiI CCNNNNNNNGG 3 cut(s) 319, 354, 355
AgsI TTSAA 4 cut(s) 14, 64, 179, 288
AjiI CACGTC 1 cut(s) 38
AluBI AGCT 3 cut(s) 216, 283, 468
AluI AGCT 3 cut(s) 216, 283, 468
Alw26I GTCTC 2 cut(s) 407, 487
AoxI GGCC 4 cut(s) 3, 23, 78, 306
ApeKI GCWGC 1 cut(s) 203
ApoI RAATTY 2 cut(s) 41, 143
AseI ATTAAT 1 cut(s) 423
AspLEI GCGC 2 cut(s) 476, 504
AspS9I GGNCC 3 cut(s) 23, 79, 358
AsuC2I CCSGG 1 cut(s) 149
AsuHPI GGTGA 1 cut(s) 118
AvaII GGWCC 1 cut(s) 358
BbvI GCAGC 1 cut(s) 190
BccI CCATC 1 cut(s) 197
BceAI ACGGC 2 cut(s) 321, 352
BcnI CCSGG 1 cut(s) 149
BcoDI GTCTC 2 cut(s) 407, 487
BisI GCNGC 3 cut(s) 78, 204, 505
BlsI GCNGC 3 cut(s) 79, 205, 506
Bme1390I CCNGG 1 cut(s) 149
Bme18I GGWCC 1 cut(s) 358
BmgBI CACGTC 1 cut(s) 38
BmgT120I GGNCC 3 cut(s) 23, 79, 358
BmiI GGNNCC 3 cut(s) 81, 247, 359
BmrFI CCNGG 1 cut(s) 149
BpuEI CTTGAG 1 cut(s) 511
BpuMI CCSGG 1 cut(s) 149
BsaAI YACGTR 1 cut(s) 373
BsaJI CCNNGG 2 cut(s) 318, 353
Bsc4I CCNNNNNNNGG 3 cut(s) 319, 354, 355
Bse118I RCCGGY 1 cut(s) 517
Bse1I ACTGG 1 cut(s) 333
BseDI CCNNGG 2 cut(s) 318, 353
BseGI GGATG 1 cut(s) 132
BseLI CCNNNNNNNGG 3 cut(s) 319, 354, 355
BseMII CTCAG 2 cut(s) 226, 427
BseNI ACTGG 1 cut(s) 333
BseXI GCAGC 1 cut(s) 190
BseYI CCCAGC 1 cut(s) 348
Bsh1236I CGCG 2 cut(s) 474, 502
BshFI GGCC 4 cut(s) 5, 25, 80, 308
BsiSI CCGG 2 cut(s) 149, 518
BsiWI CGTACG 1 cut(s) 369
BslFI GGGAC 1 cut(s) 371
BslI CCNNNNNNNGG 3 cut(s) 319, 354, 355
BsmAI GTCTC 2 cut(s) 407, 487
BsmFI GGGAC 1 cut(s) 371
BsnI GGCC 4 cut(s) 5, 25, 80, 308
Bsp1407I TGTACA 1 cut(s) 70
Bsp143I GATC 1 cut(s) 138
BspACI CCGC 2 cut(s) 77, 505
BspANI GGCC 4 cut(s) 5, 25, 80, 308
BspCNI CTCAG 2 cut(s) 225, 426
BspFNI CGCG 2 cut(s) 474, 502
BspLI GGNNCC 3 cut(s) 81, 247, 359
BsrFI RCCGGY 1 cut(s) 517
BsrGI TGTACA 1 cut(s) 70
BsrI ACTGG 1 cut(s) 333
BssAI RCCGGY 1 cut(s) 517
BssECI CCNNGG 2 cut(s) 318, 353
BssMI GATC 1 cut(s) 138
BssNAI GTATAC 2 cut(s) 303, 390
BssT1I CCWWGG 1 cut(s) 353
Bst1107I GTATAC 2 cut(s) 303, 390
Bst4CI ACNGT 2 cut(s) 401, 513
BstAUI TGTACA 1 cut(s) 70
BstBAI YACGTR 1 cut(s) 373
BstDEI CTNAG 2 cut(s) 212, 413
BstDSI CCRYGG 1 cut(s) 318
BstF5I GGATG 1 cut(s) 132
BstFNI CGCG 2 cut(s) 474, 502
BstHHI GCGC 2 cut(s) 476, 504
BstKTI GATC 1 cut(s) 141
BstMAI GTCTC 2 cut(s) 407, 487
BstMBI GATC 1 cut(s) 138
BstMWI GCNNNNNNNGC 2 cut(s) 245, 349
BstNSI RCATGY 1 cut(s) 396
BstSCI CCNGG 1 cut(s) 147
BstUI CGCG 2 cut(s) 474, 502
BstV1I GCAGC 1 cut(s) 190
BstZ17I GTATAC 2 cut(s) 303, 390
BsuRI GGCC 4 cut(s) 5, 25, 80, 308
BtgI CCRYGG 1 cut(s) 318
BtgZI GCGATG 1 cut(s) 430
BtrI CACGTC 1 cut(s) 38
BtsCI GGATG 1 cut(s) 132
BtsIMutI CAGTG 2 cut(s) 326, 397
CfoI GCGC 2 cut(s) 476, 504
Cfr10I RCCGGY 1 cut(s) 517
Cfr13I GGNCC 3 cut(s) 23, 79, 358
Csp6I GTAC 2 cut(s) 71, 370
CviAII CATG 2 cut(s) 199, 393
CviQI GTAC 2 cut(s) 71, 370
DdeI CTNAG 2 cut(s) 212, 413
DpnI GATC 1 cut(s) 140
DpnII GATC 1 cut(s) 138
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 353
Eco47I GGWCC 1 cut(s) 358
EcoT14I CCWWGG 1 cut(s) 353
ErhI CCWWGG 1 cut(s) 353
FaeI CATG 2 cut(s) 202, 396
FaiI YATR 6 cut(s) 48, 110, 200, 303, 390, 394
FaqI GGGAC 1 cut(s) 371
FatI CATG 2 cut(s) 198, 392
FblI GTMKAC 3 cut(s) 302, 389, 481
Fnu4HI GCNGC 3 cut(s) 78, 204, 505
FokI GGATG 1 cut(s) 119
Fsp4HI GCNGC 3 cut(s) 78, 204, 505
GlaI GCGC 2 cut(s) 475, 503
GluI GCNGC 3 cut(s) 78, 204, 505
GsaI CCCAGC 1 cut(s) 352
HaeIII GGCC 4 cut(s) 5, 25, 80, 308
HapII CCGG 2 cut(s) 149, 518
HhaI GCGC 2 cut(s) 476, 504
Hin1II CATG 2 cut(s) 202, 396
Hin6I GCGC 2 cut(s) 474, 502
HinP1I GCGC 2 cut(s) 474, 502
HincII GTYRAC 1 cut(s) 482
HindII GTYRAC 1 cut(s) 482
HindIII AAGCTT 1 cut(s) 281
HpaII CCGG 2 cut(s) 149, 518
HphI GGTGA 1 cut(s) 118
Hpy166II GTNNAC 3 cut(s) 303, 390, 482
Hpy188I TCNGA 1 cut(s) 416
Hpy188III TCNNGA 1 cut(s) 136
Hpy8I GTNNAC 3 cut(s) 303, 390, 482
Hpy99I CGWCG 2 cut(s) 39, 483
HpyAV CCTTC 4 cut(s) 93, 100, 139, 235
HpyCH4III ACNGT 2 cut(s) 401, 513
HpyCH4IV ACGT 3 cut(s) 37, 66, 372
HpyF10VI GCNNNNNNNGC 2 cut(s) 245, 349
HpyF3I CTNAG 2 cut(s) 212, 413
HpySE526I ACGT 3 cut(s) 37, 66, 372
Hsp92II CATG 2 cut(s) 202, 396
HspAI GCGC 2 cut(s) 474, 502
Kzo9I GATC 1 cut(s) 138
LmnI GCTCC 2 cut(s) 245, 280
LpnPI CCDG 5 cut(s) 149, 162, 314, 362, 413
Lsp1109I GCAGC 1 cut(s) 190
MaeII ACGT 3 cut(s) 37, 66, 372
MaeIII GTNAC 1 cut(s) 373
MalI GATC 1 cut(s) 140
MboI GATC 1 cut(s) 138
MboII GAAGA 2 cut(s) 52, 253
MluCI AATT 2 cut(s) 41, 143
MnlI CCTC 3 cut(s) 177, 319, 323
MseI TTAA 1 cut(s) 423
MspI CCGG 2 cut(s) 149, 518
MspR9I CCNGG 1 cut(s) 149
MvnI CGCG 2 cut(s) 474, 502
MwoI GCNNNNNNNGC 2 cut(s) 245, 349
NciI CCSGG 1 cut(s) 149
NdeII GATC 1 cut(s) 138
NlaIII CATG 2 cut(s) 202, 396
NlaIV GGNNCC 3 cut(s) 81, 247, 359
NmeAIII GCCGAG 1 cut(s) 384
NmuCI GTSAC 1 cut(s) 373
NspI RCATGY 1 cut(s) 396
PcsI WCGNNNNNNNCGW 1 cut(s) 478
Pfl23II CGTACG 1 cut(s) 369
PkrI GCNGC 3 cut(s) 79, 205, 506
Ppu21I YACGTR 1 cut(s) 373
PshBI ATTAAT 1 cut(s) 423
PsiI TTATAA 1 cut(s) 48
Psp1406I AACGTT 1 cut(s) 66
PspFI CCCAGC 1 cut(s) 348
PspLI CGTACG 1 cut(s) 369
PspN4I GGNNCC 3 cut(s) 81, 247, 359
PspPI GGNCC 3 cut(s) 23, 79, 358
RsaI GTAC 2 cut(s) 72, 371
RsaNI GTAC 2 cut(s) 71, 370
SalI GTCGAC 1 cut(s) 480
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 3 cut(s) 78, 204, 505
Sau3AI GATC 1 cut(s) 138
Sau96I GGNCC 3 cut(s) 23, 79, 358
ScrFI CCNGG 1 cut(s) 149
SinI GGWCC 1 cut(s) 358
SmlI CTYRAG 1 cut(s) 490
SmoI CTYRAG 1 cut(s) 490
Sse9I AATT 2 cut(s) 41, 143
SsiI CCGC 2 cut(s) 77, 505
SspI AATATT 1 cut(s) 421
StyD4I CCNGG 1 cut(s) 147
StyI CCWWGG 1 cut(s) 353
TaaI ACNGT 2 cut(s) 401, 513
TaiI ACGT 3 cut(s) 40, 69, 375
TaqI TCGA 2 cut(s) 156, 481
TasI AATT 2 cut(s) 41, 143
TatI WGTACW 1 cut(s) 70
TauI GCSGC 2 cut(s) 80, 507
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TscAI CASTG 2 cut(s) 333, 404
TseFI GTSAC 1 cut(s) 373
TseI GCWGC 1 cut(s) 203
Tsp45I GTSAC 1 cut(s) 373
TspDTI ATGAA 3 cut(s) 97, 121, 187
TspGWI ACGGA 1 cut(s) 307
TspRI CASTG 2 cut(s) 333, 404
VpaK11BI GGWCC 1 cut(s) 358
VspI ATTAAT 1 cut(s) 423
XapI RAATTY 2 cut(s) 41, 143
XceI RCATGY 1 cut(s) 396
XcmI CCANNNNNNNNNTGG 1 cut(s) 196
XmiI GTMKAC 3 cut(s) 302, 389, 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.