Rh1BG190900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000574
Physical Location & Seq
Reverse (-)
30614860 .. 30615393
534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG190900.1

Sequence Viewer

Length: 534 bp
ATGGCTATTTATCGTCTTTTTATTGGAATGAGTAATCCTTCAAATGTCAATATGTGCTTCTTCGTCAACAATCAATGGTACACGAACGCATCTTCAATCTATTCTAGCCATCACGACGTTGTTACTTTCACTGTTCCGATACTCCTCACCTTCATCCAGATCAGATATCCAGCAGAGAATCTGTTCCAAACTCACCCTGTCACCATCATCCTTGTCCTGCTCAGCTTACTCGCACATTGCTTGTCCTTAATGGGATTCCGGATTCTTACAAGCCAAAGTCATGATGTTGATTGTGATCGATATGCTGGAGCTCGTAAGTGGTGCAGCCTGGGCATGATATTCTTTGGTTTTGTCTTAGTTTCGTTACTCGCGGCCCTTCTTTTTCCGGTCATCTTATGGATATGTCAGTTAGTGTTGCTGCTGCCGGCACTTCTTGGTTTGGTCTTGCTGGTGGTTAAATTCCTTTGGCTACAGGGCAAAAGGAGGAGACTACCACTTTTGCCACTGACGTCAACGGATTTGCTGCGCGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

177

Amino Acids

20.17

Weight (kDa)

9.17

Isoelectric Point (pI)

46.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 512
AccII CGCG 2 cut(s) 371, 528
AccIII TCCGGA 1 cut(s) 258
AciI CCGC 1 cut(s) 371
AcsI RAATTY 1 cut(s) 458
AcyI GRCGYC 1 cut(s) 509
AfaI GTAC 1 cut(s) 80
AgsI TTSAA 2 cut(s) 42, 96
AjnI CCWGG 1 cut(s) 327
AluBI AGCT 2 cut(s) 225, 311
AluI AGCT 2 cut(s) 225, 311
Alw21I GWGCWC 1 cut(s) 313
Alw26I GTCTC 1 cut(s) 481
Aor13HI TCCGGA 1 cut(s) 258
AoxI GGCC 1 cut(s) 372
ApeKI GCWGC 4 cut(s) 324, 418, 421, 523
ApoI RAATTY 1 cut(s) 458
Asp700I GAANNNNTTC 1 cut(s) 182
AspLEI GCGC 1 cut(s) 528
AspS9I GGNCC 1 cut(s) 373
AsuHPI GGTGA 3 cut(s) 139, 185, 193
BanII GRGCYC 1 cut(s) 313
Bbv12I GWGCWC 1 cut(s) 313
BbvI GCAGC 4 cut(s) 336, 405, 408, 510
BccI CCATC 2 cut(s) 117, 212
BciT130I CCWGG 1 cut(s) 329
BcoDI GTCTC 1 cut(s) 481
BfaI CTAG 1 cut(s) 105
BfmI CTRYAG 1 cut(s) 470
BisI GCNGC 5 cut(s) 325, 372, 419, 422, 524
BlpI GCTNAGC 1 cut(s) 221
BlsI GCNGC 5 cut(s) 326, 373, 420, 423, 525
Bme1390I CCNGG 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 373
BmrFI CCNGG 1 cut(s) 329
BmsI GCATC 1 cut(s) 98
BpmI CTGGAG 1 cut(s) 327
Bpu1102I GCTNAGC 1 cut(s) 221
Bsa29I ATCGAT 1 cut(s) 298
BsaBI GATNNNNATC 1 cut(s) 294
BsaHI GRCGYC 1 cut(s) 509
BsaJI CCNNGG 1 cut(s) 328
BsaWI WCCGGW 2 cut(s) 258, 385
Bse118I RCCGGY 1 cut(s) 424
Bse3DI GCAATG 1 cut(s) 235
Bse8I GATNNNNATC 1 cut(s) 294
BseAI TCCGGA 1 cut(s) 258
BseBI CCWGG 1 cut(s) 329
BseCI ATCGAT 1 cut(s) 298
BseDI CCNNGG 1 cut(s) 328
BseGI GGATG 2 cut(s) 153, 207
BseJI GATNNNNATC 1 cut(s) 294
BseMI GCAATG 1 cut(s) 235
BseMII CTCAG 1 cut(s) 235
BseRI GAGGAG 2 cut(s) 134, 499
BseXI GCAGC 4 cut(s) 336, 405, 408, 510
BsgI GTGCAG 1 cut(s) 343
Bsh1236I CGCG 2 cut(s) 371, 528
BshFI GGCC 1 cut(s) 374
BshVI ATCGAT 1 cut(s) 298
BsiHKAI GWGCWC 1 cut(s) 313
BsiSI CCGG 3 cut(s) 259, 386, 425
BsmAI GTCTC 1 cut(s) 481
BsnI GGCC 1 cut(s) 374
Bsp1286I GDGCHC 1 cut(s) 313
Bsp13I TCCGGA 1 cut(s) 258
Bsp143I GATC 2 cut(s) 159, 295
Bsp1720I GCTNAGC 1 cut(s) 221
BspACI CCGC 1 cut(s) 371
BspANI GGCC 1 cut(s) 374
BspCNI CTCAG 1 cut(s) 234
BspDI ATCGAT 1 cut(s) 298
BspEI TCCGGA 1 cut(s) 258
BspFNI CGCG 2 cut(s) 371, 528
BspHI TCATGA 1 cut(s) 280
BsrDI GCAATG 1 cut(s) 235
BsrFI RCCGGY 1 cut(s) 424
BssAI RCCGGY 1 cut(s) 424
BssECI CCNNGG 1 cut(s) 328
BssMI GATC 2 cut(s) 159, 295
BssNI GRCGYC 1 cut(s) 509
Bst2UI CCWGG 1 cut(s) 329
Bst4CI ACNGT 1 cut(s) 133
BstACI GRCGYC 1 cut(s) 509
BstC8I GCNNGC 1 cut(s) 426
BstDEI CTNAG 2 cut(s) 221, 355
BstF5I GGATG 2 cut(s) 153, 207
BstFNI CGCG 2 cut(s) 371, 528
BstHHI GCGC 1 cut(s) 528
BstKTI GATC 2 cut(s) 162, 298
BstMAI GTCTC 1 cut(s) 481
BstMBI GATC 2 cut(s) 159, 295
BstMWI GCNNNNNNNGC 1 cut(s) 330
BstNI CCWGG 1 cut(s) 329
BstSCI CCNGG 1 cut(s) 327
BstSFI CTRYAG 1 cut(s) 470
BstUI CGCG 2 cut(s) 371, 528
BstV1I GCAGC 4 cut(s) 336, 405, 408, 510
Bsu15I ATCGAT 1 cut(s) 298
BsuRI GGCC 1 cut(s) 374
BsuTUI ATCGAT 1 cut(s) 298
BtsCI GGATG 2 cut(s) 153, 207
BtsIMutI CAGTG 2 cut(s) 129, 503
Cac8I GCNNGC 1 cut(s) 426
CciI TCATGA 1 cut(s) 280
CfoI GCGC 1 cut(s) 528
Cfr10I RCCGGY 1 cut(s) 424
Cfr13I GGNCC 1 cut(s) 373
ClaI ATCGAT 1 cut(s) 298
Csp6I GTAC 1 cut(s) 79
CviAII CATG 2 cut(s) 281, 334
CviJI RGCY 8 cut(s) 5, 108, 225, 273, 311, 327, 374, 469
CviKI_1 RGCY 8 cut(s) 5, 108, 225, 273, 311, 327, 374, 469
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 2 cut(s) 221, 355
DpnI GATC 2 cut(s) 161, 297
DpnII GATC 2 cut(s) 159, 295
Ecl136II GAGCTC 1 cut(s) 311
Eco24I GRGCYC 1 cut(s) 313
Eco32I GATATC 1 cut(s) 167
Eco53kI GAGCTC 1 cut(s) 311
EcoICRI GAGCTC 1 cut(s) 311
EcoRII CCWGG 1 cut(s) 327
EcoRV GATATC 1 cut(s) 167
EcoT38I GRGCYC 1 cut(s) 313
FaeI CATG 2 cut(s) 284, 337
FaiI YATR 6 cut(s) 53, 282, 303, 335, 397, 403
FatI CATG 2 cut(s) 280, 333
Fnu4HI GCNGC 5 cut(s) 325, 372, 419, 422, 524
FokI GGATG 2 cut(s) 140, 194
FriOI GRGCYC 1 cut(s) 313
Fsp4HI GCNGC 5 cut(s) 325, 372, 419, 422, 524
FspBI CTAG 1 cut(s) 105
GlaI GCGC 1 cut(s) 527
GluI GCNGC 5 cut(s) 325, 372, 419, 422, 524
GsuI CTGGAG 1 cut(s) 327
HaeIII GGCC 1 cut(s) 374
HapII CCGG 3 cut(s) 259, 386, 425
HhaI GCGC 1 cut(s) 528
Hin1I GRCGYC 1 cut(s) 509
Hin1II CATG 2 cut(s) 284, 337
Hin6I GCGC 1 cut(s) 526
HinP1I GCGC 1 cut(s) 526
HincII GTYRAC 2 cut(s) 67, 513
HindII GTYRAC 2 cut(s) 67, 513
HinfI GANTC 3 cut(s) 178, 255, 262
HpaII CCGG 3 cut(s) 259, 386, 425
HphI GGTGA 3 cut(s) 139, 185, 193
Hpy166II GTNNAC 3 cut(s) 67, 81, 513
Hpy188I TCNGA 2 cut(s) 138, 164
Hpy188III TCNNGA 4 cut(s) 113, 157, 259, 281
Hpy8I GTNNAC 3 cut(s) 67, 81, 513
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 3 cut(s) 48, 160, 386
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4IV ACGT 2 cut(s) 117, 509
HpyCH4V TGCA 1 cut(s) 324
HpyF10VI GCNNNNNNNGC 1 cut(s) 330
HpyF3I CTNAG 2 cut(s) 221, 355
HpySE526I ACGT 2 cut(s) 117, 509
Hsp92I GRCGYC 1 cut(s) 509
Hsp92II CATG 2 cut(s) 284, 337
HspAI GCGC 1 cut(s) 526
Kpn2I TCCGGA 1 cut(s) 258
KroI GCCGGC 1 cut(s) 424
KroNI GCCGGC 1 cut(s) 426
Kzo9I GATC 2 cut(s) 159, 295
LmnI GCTCC 1 cut(s) 308
Lsp1109I GCAGC 4 cut(s) 336, 405, 408, 510
LweI GCATC 1 cut(s) 98
MaeI CTAG 1 cut(s) 105
MaeII ACGT 2 cut(s) 117, 509
MaeIII GTNAC 3 cut(s) 121, 199, 363
MalI GATC 2 cut(s) 161, 297
MboI GATC 2 cut(s) 159, 295
MboII GAAGA 2 cut(s) 52, 84
MhlI GDGCHC 1 cut(s) 313
MluCI AATT 1 cut(s) 458
MnlI CCTC 2 cut(s) 155, 477
MroI TCCGGA 1 cut(s) 258
MroNI GCCGGC 1 cut(s) 424
MroXI GAANNNNTTC 1 cut(s) 182
MseI TTAA 2 cut(s) 248, 456
MspI CCGG 3 cut(s) 259, 386, 425
MspR9I CCNGG 1 cut(s) 329
MvaI CCWGG 1 cut(s) 329
MvnI CGCG 2 cut(s) 371, 528
MwoI GCNNNNNNNGC 1 cut(s) 330
NaeI GCCGGC 1 cut(s) 426
NdeII GATC 2 cut(s) 159, 295
NgoMIV GCCGGC 1 cut(s) 424
NlaIII CATG 2 cut(s) 284, 337
NmuCI GTSAC 1 cut(s) 199
PagI TCATGA 1 cut(s) 280
PdiI GCCGGC 1 cut(s) 426
PdmI GAANNNNTTC 1 cut(s) 182
PfeI GAWTC 3 cut(s) 178, 255, 262
PkrI GCNGC 5 cut(s) 326, 373, 420, 423, 525
Psp124BI GAGCTC 1 cut(s) 313
Psp6I CCWGG 1 cut(s) 327
PspGI CCWGG 1 cut(s) 327
PspPI GGNCC 1 cut(s) 373
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
SacI GAGCTC 1 cut(s) 313
SaqAI TTAA 2 cut(s) 248, 456
SatI GCNGC 5 cut(s) 325, 372, 419, 422, 524
Sau3AI GATC 2 cut(s) 159, 295
Sau96I GGNCC 1 cut(s) 373
ScrFI CCNGG 1 cut(s) 329
SduI GDGCHC 1 cut(s) 313
SetI ASST 5 cut(s) 120, 152, 227, 313, 512
SfaNI GCATC 1 cut(s) 98
SfcI CTRYAG 1 cut(s) 470
Sse9I AATT 1 cut(s) 458
SsiI CCGC 1 cut(s) 371
SspMI CTAG 1 cut(s) 105
SstI GAGCTC 1 cut(s) 313
StyD4I CCNGG 1 cut(s) 327
TaaI ACNGT 1 cut(s) 133
TaiI ACGT 2 cut(s) 120, 512
TaqI TCGA 1 cut(s) 298
TasI AATT 1 cut(s) 458
TauI GCSGC 1 cut(s) 374
TfiI GAWTC 3 cut(s) 178, 255, 262
Tru1I TTAA 2 cut(s) 248, 456
Tru9I TTAA 2 cut(s) 248, 456
TscAI CASTG 2 cut(s) 136, 510
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 4 cut(s) 324, 418, 421, 523
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 1 cut(s) 142
TspGWI ACGGA 1 cut(s) 530
TspRI CASTG 2 cut(s) 136, 510
XapI RAATTY 1 cut(s) 458
XmnI GAANNNNTTC 1 cut(s) 182
XspI CTAG 1 cut(s) 105
ZraI GACGTC 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.