RLG00000028520

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000574
Physical Location & Seq
Forward (+)
23851127 .. 23851792
666 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028520

Sequence Viewer

Length: 552 bp
ATGGGAGTCATGCACTCCATCATTAACAACATTCAGTTCCAGAACTTTGGACAGATATTAATTCTGCCGGGAACTGCTTCCAGATATTCTTACAGGTATAGTCATGTTCACGACGCCGTTGTGTTCATCATTCCGGTACTCCTGACCTTGGTCCAGATCAGATATCCGGCTGAAAATCTGTTCACCACACACCCCATCACCATCATCTTTGTTCAGTCTAGCTTACTTGGATATTGCTTGGTCTTAAGCTTCTGGGTACTTATAGGTCAAACTCAGAGTTATAGTCATAGATGTGATGCAAAATATTATTGGTGCTGCATAGCCAAATTGGTATTTGGTTCAGTTACAGTTTTTTCACTCATCTGGCTTCTGCTGGACGAGGCTGTGCTTTATCTTTTGTGGATGGGTCAGCTGTTGCTAGTATTTATATTCCTCCTCAAGCTAGTACTGCTTCTCCGTAAAATCTTTCCTTCTTTGTTGTGTCCGGAGCGGCAAAATCGAAAAGGGAGAACATATCCACTTTTGCCGCTGACCACAGTCGATTTGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

184

Amino Acids

21.1

Weight (kDa)

9.18

Isoelectric Point (pI)

32.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 490
AccIII TCCGGA 1 cut(s) 484
AciI CCGC 2 cut(s) 490, 527
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 3 cut(s) 138, 258, 447
AfiI CCNNNNNNNGG 1 cut(s) 148
AflII CTTAAG 1 cut(s) 244
AluBI AGCT 4 cut(s) 222, 249, 412, 442
AluI AGCT 4 cut(s) 222, 249, 412, 442
Aor13HI TCCGGA 1 cut(s) 484
AoxI GGCC 1 cut(s) 546
ApeKI GCWGC 1 cut(s) 315
AseI ATTAAT 1 cut(s) 59
Asp700I GAANNNNTTC 1 cut(s) 76
AspS9I GGNCC 1 cut(s) 151
AsuC2I CCSGG 1 cut(s) 69
AsuHPI GGTGA 2 cut(s) 175, 190
AvaII GGWCC 1 cut(s) 151
BbvI GCAGC 1 cut(s) 302
BccI CCATC 4 cut(s) 26, 203, 209, 397
BceAI ACGGC 1 cut(s) 101
BcnI CCSGG 1 cut(s) 69
BfaI CTAG 3 cut(s) 219, 419, 443
BfrI CTTAAG 1 cut(s) 244
BisI GCNGC 3 cut(s) 316, 491, 527
BlsI GCNGC 3 cut(s) 317, 492, 528
BmcAI AGTACT 1 cut(s) 447
Bme1390I CCNGG 1 cut(s) 69
Bme18I GGWCC 1 cut(s) 151
BmgT120I GGNCC 1 cut(s) 151
BmrFI CCNGG 1 cut(s) 69
BmsI GCATC 1 cut(s) 286
BoxI GACNNNNGTC 2 cut(s) 149, 536
BpuEI CTTGAG 1 cut(s) 422
BpuMI CCSGG 1 cut(s) 69
BsaHI GRCGYC 1 cut(s) 114
BsaJI CCNNGG 1 cut(s) 147
BsaWI WCCGGW 2 cut(s) 133, 484
BsaXI ACNNNNNCTCC 2 cut(s) 438, 468
Bsc4I CCNNNNNNNGG 1 cut(s) 148
BseAI TCCGGA 1 cut(s) 484
BseDI CCNNGG 1 cut(s) 147
BseGI GGATG 1 cut(s) 408
BseLI CCNNNNNNNGG 1 cut(s) 148
BseMII CTCAG 1 cut(s) 287
BseRI GAGGAG 1 cut(s) 425
BseXI GCAGC 1 cut(s) 302
BshFI GGCC 1 cut(s) 548
BsiSI CCGG 4 cut(s) 68, 134, 167, 485
BslI CCNNNNNNNGG 1 cut(s) 148
BsnI GGCC 1 cut(s) 548
Bsp13I TCCGGA 1 cut(s) 484
Bsp143I GATC 1 cut(s) 156
BspACI CCGC 2 cut(s) 490, 527
BspANI GGCC 1 cut(s) 548
BspCNI CTCAG 1 cut(s) 286
BspEI TCCGGA 1 cut(s) 484
BspTI CTTAAG 1 cut(s) 244
BsrBI CCGCTC 1 cut(s) 490
BssECI CCNNGG 1 cut(s) 147
BssMI GATC 1 cut(s) 156
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 1 cut(s) 147
Bst4CI ACNGT 2 cut(s) 349, 538
BstACI GRCGYC 1 cut(s) 114
BstAFI CTTAAG 1 cut(s) 244
BstDEI CTNAG 1 cut(s) 273
BstF5I GGATG 1 cut(s) 408
BstKTI GATC 1 cut(s) 159
BstMBI GATC 1 cut(s) 156
BstMWI GCNNNNNNNGC 1 cut(s) 448
BstPAI GACNNNNGTC 2 cut(s) 149, 536
BstSCI CCNGG 1 cut(s) 67
BstV1I GCAGC 1 cut(s) 302
BstXI CCANNNNNNTGG 1 cut(s) 47
BsuRI GGCC 1 cut(s) 548
BtsCI GGATG 1 cut(s) 408
Cfr13I GGNCC 1 cut(s) 151
CseI GACGC 1 cut(s) 122
Csp6I GTAC 3 cut(s) 137, 257, 446
CviAII CATG 2 cut(s) 10, 104
CviJI RGCY 9 cut(s) 170, 222, 249, 323, 367, 383, 412, 442, 548
CviKI_1 RGCY 9 cut(s) 170, 222, 249, 323, 367, 383, 412, 442, 548
CviQI GTAC 3 cut(s) 137, 257, 446
DdeI CTNAG 1 cut(s) 273
DpnI GATC 1 cut(s) 158
DpnII GATC 1 cut(s) 156
Eco130I CCWWGG 1 cut(s) 147
Eco32I GATATC 1 cut(s) 164
Eco47I GGWCC 1 cut(s) 151
EcoRV GATATC 1 cut(s) 164
EcoT14I CCWWGG 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 147
FaeI CATG 2 cut(s) 13, 107
FaiI YATR 9 cut(s) 11, 99, 105, 263, 282, 288, 320, 428, 514
FatI CATG 2 cut(s) 9, 103
Fnu4HI GCNGC 3 cut(s) 316, 491, 527
FokI GGATG 1 cut(s) 415
Fsp4HI GCNGC 3 cut(s) 316, 491, 527
FspBI CTAG 3 cut(s) 219, 419, 443
GluI GCNGC 3 cut(s) 316, 491, 527
HaeIII GGCC 1 cut(s) 548
HapII CCGG 4 cut(s) 68, 134, 167, 485
HgaI GACGC 1 cut(s) 122
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 2 cut(s) 13, 107
HindIII AAGCTT 1 cut(s) 247
HinfI GANTC 1 cut(s) 6
HpaII CCGG 4 cut(s) 68, 134, 167, 485
HphI GGTGA 2 cut(s) 175, 190
Hpy166II GTNNAC 2 cut(s) 109, 183
Hpy188I TCNGA 2 cut(s) 161, 276
Hpy188III TCNNGA 6 cut(s) 40, 81, 110, 142, 154, 485
Hpy8I GTNNAC 2 cut(s) 109, 183
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 1 cut(s) 480
HpyCH4III ACNGT 2 cut(s) 349, 538
HpyCH4V TGCA 3 cut(s) 13, 299, 318
HpyF10VI GCNNNNNNNGC 1 cut(s) 448
HpyF3I CTNAG 1 cut(s) 273
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 2 cut(s) 13, 107
Kpn2I TCCGGA 1 cut(s) 484
Kzo9I GATC 1 cut(s) 156
LmnI GCTCC 1 cut(s) 487
Lsp1109I GCAGC 1 cut(s) 302
LweI GCATC 1 cut(s) 286
MaeI CTAG 3 cut(s) 219, 419, 443
MaeIII GTNAC 1 cut(s) 343
MalI GATC 1 cut(s) 158
MbiI CCGCTC 1 cut(s) 490
MboI GATC 1 cut(s) 156
MluCI AATT 2 cut(s) 60, 326
MlyI GAGTC 1 cut(s) 15
MnlI CCTC 3 cut(s) 373, 443, 446
MroI TCCGGA 1 cut(s) 484
MroXI GAANNNNTTC 1 cut(s) 76
MseI TTAA 3 cut(s) 24, 59, 245
MslI CAYNNNNRTG 1 cut(s) 291
MspA1I CMGCKG 2 cut(s) 412, 529
MspCI CTTAAG 1 cut(s) 244
MspI CCGG 4 cut(s) 68, 134, 167, 485
MspR9I CCNGG 1 cut(s) 69
MwoI GCNNNNNNNGC 1 cut(s) 448
NciI CCSGG 1 cut(s) 69
NdeII GATC 1 cut(s) 156
NlaIII CATG 2 cut(s) 13, 107
PdmI GAANNNNTTC 1 cut(s) 76
PkrI GCNGC 3 cut(s) 317, 492, 528
PleI GAGTC 1 cut(s) 14
PpsI GAGTC 1 cut(s) 14
PshAI GACNNNNGTC 2 cut(s) 149, 536
PshBI ATTAAT 1 cut(s) 59
PspPI GGNCC 1 cut(s) 151
PvuII CAGCTG 1 cut(s) 412
RsaI GTAC 3 cut(s) 138, 258, 447
RsaNI GTAC 3 cut(s) 137, 257, 446
RseI CAYNNNNRTG 1 cut(s) 291
SaqAI TTAA 3 cut(s) 24, 59, 245
SatI GCNGC 3 cut(s) 316, 491, 527
Sau3AI GATC 1 cut(s) 156
Sau96I GGNCC 1 cut(s) 151
ScaI AGTACT 1 cut(s) 447
SchI GAGTC 1 cut(s) 15
ScrFI CCNGG 1 cut(s) 69
SetI ASST 7 cut(s) 98, 149, 224, 251, 268, 414, 444
SfaNI GCATC 1 cut(s) 286
SinI GGWCC 1 cut(s) 151
SmiMI CAYNNNNRTG 1 cut(s) 291
SmlI CTYRAG 2 cut(s) 244, 437
SmoI CTYRAG 2 cut(s) 244, 437
Sse9I AATT 2 cut(s) 60, 326
SsiI CCGC 2 cut(s) 490, 527
SspI AATATT 1 cut(s) 305
SspMI CTAG 3 cut(s) 219, 419, 443
StyD4I CCNGG 1 cut(s) 67
StyI CCWWGG 1 cut(s) 147
TaaI ACNGT 2 cut(s) 349, 538
TaqI TCGA 2 cut(s) 499, 540
TasI AATT 2 cut(s) 60, 326
TatI WGTACW 1 cut(s) 445
TauI GCSGC 2 cut(s) 493, 529
Tru1I TTAA 3 cut(s) 24, 59, 245
Tru9I TTAA 3 cut(s) 24, 59, 245
TseI GCWGC 1 cut(s) 315
TspDTI ATGAA 1 cut(s) 115
TspGWI ACGGA 1 cut(s) 446
Vha464I CTTAAG 1 cut(s) 244
VpaK11BI GGWCC 1 cut(s) 151
VspI ATTAAT 1 cut(s) 59
XcmI CCANNNNNNNNNTGG 1 cut(s) 541
XmnI GAANNNNTTC 1 cut(s) 76
XspI CTAG 3 cut(s) 219, 419, 443
ZrmI AGTACT 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.