RLG00000017055

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000574
Physical Location & Seq
Forward (+)
12462000 .. 12462611
612 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017055

Sequence Viewer

Length: 612 bp
ATGGCCAGTTTTCCCGATCAATGGACACCCTCGGTCTTATTACCACGTTCACGTGGTAATAATATCAACCTCATCATCAACGTTGTACAAGCGGCCCCTTCCACCTTCGCCATTTTCACTTTCATGGTGCCAATACTTCTCACCTTCATCCAGATCAAATTCCCGGCCGTCGGAACTCTATCTCTCCCATTTGAAACCCACCACACCAAGTTCATGGTTGCCATTCTCAGCTTACTTGCCTTCTGTTGCTTTGCCATTGGAGCCACACAAACTAACTTGTTCCCGAATCACGCTGCAAAACTTTCTATGGCCATCAAGTGTACGGCTTCGCTCTCCGTGGCTTCGCTTGTGTCTCTTTTGCTTCCCAAGCCTTGGGACCTAGTGCCGTACGTGACCTACTTCTTGTATGCATGCCACTGTCTCGGCCTACTCAGAAATATTAATCTGGCTTATTACAACGACATTGCTTGTGGATTCGTGCATAGCTCACGCGCCCGTCTACGGCAGTTGAGACAAACGCGCCGCCCACTGTTGCCGATGACAGTCACAGATATCTCCAACCACCCGATTGCAAACCGTGGATCAAGCACTGAAGTCAGAACAGTCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

204

Amino Acids

22.55

Weight (kDa)

9.78

Isoelectric Point (pI)

50.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 127
AccB7I CCANNNNNTGG 1 cut(s) 372
AccI GTMKAC 1 cut(s) 499
AccII CGCG 2 cut(s) 492, 520
AciI CCGC 2 cut(s) 92, 523
AclI AACGTT 1 cut(s) 81
AclWI GGATC 1 cut(s) 589
AcoI YGGCCR 3 cut(s) 3, 165, 309
AcsI RAATTY 1 cut(s) 158
AcuI CTGAAG 1 cut(s) 612
AcvI CACGTG 1 cut(s) 53
AfaI GTAC 3 cut(s) 87, 322, 389
AfiI CCNNNNNNNGG 4 cut(s) 21, 170, 372, 501
AgsI TTSAA 1 cut(s) 194
AluBI AGCT 2 cut(s) 231, 486
AluI AGCT 2 cut(s) 231, 486
Alw26I GTCTC 3 cut(s) 357, 425, 505
AlwI GGATC 1 cut(s) 589
AoxI GGCC 5 cut(s) 3, 93, 165, 309, 424
ApeKI GCWGC 1 cut(s) 293
ApoI RAATTY 1 cut(s) 158
AseI ATTAAT 1 cut(s) 441
AspLEI GCGC 2 cut(s) 494, 522
AspS9I GGNCC 2 cut(s) 94, 376
AsuC2I CCSGG 1 cut(s) 164
AsuHPI GGTGA 1 cut(s) 133
AvaII GGWCC 1 cut(s) 376
BalI TGGCCA 2 cut(s) 5, 311
BanI GGYRCC 1 cut(s) 127
BbrPI CACGTG 1 cut(s) 53
BbvI GCAGC 1 cut(s) 280
BccI CCATC 1 cut(s) 320
BceAI ACGGC 4 cut(s) 152, 339, 370, 518
BcnI CCSGG 1 cut(s) 164
BcoDI GTCTC 3 cut(s) 357, 425, 505
BfaI CTAG 1 cut(s) 380
BisI GCNGC 3 cut(s) 93, 294, 523
BlsI GCNGC 3 cut(s) 94, 295, 524
Bme1390I CCNGG 1 cut(s) 164
Bme18I GGWCC 1 cut(s) 376
BmgT120I GGNCC 2 cut(s) 94, 376
BmiI GGNNCC 4 cut(s) 96, 129, 262, 377
BmrFI CCNGG 1 cut(s) 164
BpuMI CCSGG 1 cut(s) 164
BsaAI YACGTR 2 cut(s) 53, 391
BsaJI CCNNGG 4 cut(s) 30, 336, 371, 577
Bsc4I CCNNNNNNNGG 4 cut(s) 21, 170, 372, 501
Bse1I ACTGG 1 cut(s) 6
Bse3DI GCAATG 1 cut(s) 462
BseDI CCNNGG 4 cut(s) 30, 336, 371, 577
BseGI GGATG 1 cut(s) 147
BseLI CCNNNNNNNGG 4 cut(s) 21, 170, 372, 501
BseMI GCAATG 1 cut(s) 462
BseMII CTCAG 2 cut(s) 241, 445
BseNI ACTGG 1 cut(s) 6
BseX3I CGGCCG 1 cut(s) 165
BseXI GCAGC 1 cut(s) 280
Bsh1236I CGCG 2 cut(s) 492, 520
Bsh1285I CGRYCG 1 cut(s) 168
BshFI GGCC 5 cut(s) 5, 95, 167, 311, 426
BshNI GGYRCC 1 cut(s) 127
BsiEI CGRYCG 1 cut(s) 168
BsiSI CCGG 1 cut(s) 164
BsiWI CGTACG 1 cut(s) 387
BslFI GGGAC 1 cut(s) 389
BslI CCNNNNNNNGG 4 cut(s) 21, 170, 372, 501
BsmAI GTCTC 3 cut(s) 357, 425, 505
BsmFI GGGAC 1 cut(s) 389
BsnI GGCC 5 cut(s) 5, 95, 167, 311, 426
Bsp1407I TGTACA 1 cut(s) 85
Bsp143I GATC 3 cut(s) 16, 153, 581
BspACI CCGC 2 cut(s) 92, 523
BspANI GGCC 5 cut(s) 5, 95, 167, 311, 426
BspCNI CTCAG 2 cut(s) 240, 444
BspFNI CGCG 2 cut(s) 492, 520
BspLI GGNNCC 4 cut(s) 96, 129, 262, 377
BspPI GGATC 1 cut(s) 589
BspT107I GGYRCC 1 cut(s) 127
BsrDI GCAATG 1 cut(s) 462
BsrGI TGTACA 1 cut(s) 85
BsrI ACTGG 1 cut(s) 6
BssECI CCNNGG 4 cut(s) 30, 336, 371, 577
BssMI GATC 3 cut(s) 16, 153, 581
BssT1I CCWWGG 1 cut(s) 371
Bst4CI ACNGT 5 cut(s) 419, 531, 544, 578, 604
BstAUI TGTACA 1 cut(s) 85
BstBAI YACGTR 2 cut(s) 53, 391
BstC8I GCNNGC 1 cut(s) 412
BstDEI CTNAG 2 cut(s) 227, 431
BstDSI CCRYGG 2 cut(s) 336, 577
BstF5I GGATG 1 cut(s) 147
BstFNI CGCG 2 cut(s) 492, 520
BstHHI GCGC 2 cut(s) 494, 522
BstKTI GATC 3 cut(s) 19, 156, 584
BstMAI GTCTC 3 cut(s) 357, 425, 505
BstMBI GATC 3 cut(s) 16, 153, 581
BstMCI CGRYCG 1 cut(s) 168
BstMWI GCNNNNNNNGC 2 cut(s) 260, 367
BstNSI RCATGY 1 cut(s) 414
BstSCI CCNGG 1 cut(s) 162
BstUI CGCG 2 cut(s) 492, 520
BstV1I GCAGC 1 cut(s) 280
BstXI CCANNNNNNTGG 1 cut(s) 214
BstZI CGGCCG 1 cut(s) 165
BsuRI GGCC 5 cut(s) 5, 95, 167, 311, 426
BtgI CCRYGG 2 cut(s) 336, 577
BtsCI GGATG 1 cut(s) 147
BtsIMutI CAGTG 3 cut(s) 415, 527, 588
Cac8I GCNNGC 1 cut(s) 412
CfoI GCGC 2 cut(s) 494, 522
Cfr13I GGNCC 2 cut(s) 94, 376
Csp6I GTAC 3 cut(s) 86, 321, 388
CviAII CATG 3 cut(s) 124, 214, 411
CviQI GTAC 3 cut(s) 86, 321, 388
DdeI CTNAG 2 cut(s) 227, 431
DpnI GATC 3 cut(s) 18, 155, 583
DpnII GATC 3 cut(s) 16, 153, 581
EaeI YGGCCR 3 cut(s) 3, 165, 309
EagI CGGCCG 1 cut(s) 165
EclXI CGGCCG 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 371
Eco32I GATATC 1 cut(s) 553
Eco47I GGWCC 1 cut(s) 376
Eco52I CGGCCG 1 cut(s) 165
Eco57I CTGAAG 1 cut(s) 612
Eco72I CACGTG 1 cut(s) 53
EcoO109I RGGNCCY 1 cut(s) 376
EcoRV GATATC 1 cut(s) 553
EcoT14I CCWWGG 1 cut(s) 371
EcoT22I ATGCAT 1 cut(s) 412
ErhI CCWWGG 1 cut(s) 371
FaeI CATG 3 cut(s) 127, 217, 414
FaiI YATR 6 cut(s) 125, 215, 308, 408, 412, 483
FaqI GGGAC 1 cut(s) 389
FatI CATG 3 cut(s) 123, 213, 410
FblI GTMKAC 1 cut(s) 499
Fnu4HI GCNGC 3 cut(s) 93, 294, 523
FokI GGATG 1 cut(s) 134
Fsp4HI GCNGC 3 cut(s) 93, 294, 523
FspBI CTAG 1 cut(s) 380
GlaI GCGC 2 cut(s) 493, 521
GluI GCNGC 3 cut(s) 93, 294, 523
HaeIII GGCC 5 cut(s) 5, 95, 167, 311, 426
HapII CCGG 1 cut(s) 164
HhaI GCGC 2 cut(s) 494, 522
Hin1II CATG 3 cut(s) 127, 217, 414
Hin6I GCGC 2 cut(s) 492, 520
HinP1I GCGC 2 cut(s) 492, 520
HinfI GANTC 2 cut(s) 286, 474
HpaII CCGG 1 cut(s) 164
HphI GGTGA 1 cut(s) 133
Hpy166II GTNNAC 4 cut(s) 50, 321, 500, 607
Hpy188I TCNGA 3 cut(s) 173, 434, 599
Hpy188III TCNNGA 3 cut(s) 14, 151, 283
Hpy8I GTNNAC 4 cut(s) 50, 321, 500, 607
Hpy99I CGWCG 1 cut(s) 173
HpyAV CCTTC 4 cut(s) 108, 115, 154, 250
HpyCH4III ACNGT 5 cut(s) 419, 531, 544, 578, 604
HpyCH4IV ACGT 4 cut(s) 46, 52, 81, 390
HpyCH4V TGCA 4 cut(s) 296, 410, 481, 572
HpyF10VI GCNNNNNNNGC 2 cut(s) 260, 367
HpyF3I CTNAG 2 cut(s) 227, 431
HpySE526I ACGT 4 cut(s) 46, 52, 81, 390
Hsp92II CATG 3 cut(s) 127, 217, 414
HspAI GCGC 2 cut(s) 492, 520
Kzo9I GATC 3 cut(s) 16, 153, 581
LmnI GCTCC 1 cut(s) 260
LpnPI CCDG 4 cut(s) 19, 164, 177, 431
Lsp1109I GCAGC 1 cut(s) 280
MaeI CTAG 1 cut(s) 380
MaeII ACGT 4 cut(s) 46, 52, 81, 390
MaeIII GTNAC 2 cut(s) 391, 544
MalI GATC 3 cut(s) 18, 155, 583
MboI GATC 3 cut(s) 16, 153, 581
MlsI TGGCCA 2 cut(s) 5, 311
MluCI AATT 1 cut(s) 158
MluNI TGGCCA 2 cut(s) 5, 311
MmeI TCCRAC 2 cut(s) 151, 582
MnlI CCTC 2 cut(s) 40, 80
Mox20I TGGCCA 2 cut(s) 5, 311
Mph1103I ATGCAT 1 cut(s) 412
MscI TGGCCA 2 cut(s) 5, 311
MseI TTAA 1 cut(s) 441
MslI CAYNNNNRTG 1 cut(s) 122
Msp20I TGGCCA 2 cut(s) 5, 311
MspI CCGG 1 cut(s) 164
MspR9I CCNGG 1 cut(s) 164
MvnI CGCG 2 cut(s) 492, 520
MwoI GCNNNNNNNGC 2 cut(s) 260, 367
NciI CCSGG 1 cut(s) 164
NdeII GATC 3 cut(s) 16, 153, 581
NlaIII CATG 3 cut(s) 127, 217, 414
NlaIV GGNNCC 4 cut(s) 96, 129, 262, 377
NmeAIII GCCGAG 1 cut(s) 402
NmuCI GTSAC 2 cut(s) 391, 544
NsiI ATGCAT 1 cut(s) 412
NspI RCATGY 1 cut(s) 414
PaeI GCATGC 1 cut(s) 414
PfeI GAWTC 2 cut(s) 286, 474
Pfl23II CGTACG 1 cut(s) 387
PflMI CCANNNNNTGG 1 cut(s) 372
PkrI GCNGC 3 cut(s) 94, 295, 524
PmaCI CACGTG 1 cut(s) 53
PmlI CACGTG 1 cut(s) 53
Ppu21I YACGTR 2 cut(s) 53, 391
PpuMI RGGWCCY 1 cut(s) 376
PshBI ATTAAT 1 cut(s) 441
Psp1406I AACGTT 1 cut(s) 81
Psp5II RGGWCCY 1 cut(s) 376
PspCI CACGTG 1 cut(s) 53
PspLI CGTACG 1 cut(s) 387
PspN4I GGNNCC 4 cut(s) 96, 129, 262, 377
PspPI GGNCC 2 cut(s) 94, 376
PspPPI RGGWCCY 1 cut(s) 376
RsaI GTAC 3 cut(s) 87, 322, 389
RsaNI GTAC 3 cut(s) 86, 321, 388
RseI CAYNNNNRTG 1 cut(s) 122
SaqAI TTAA 1 cut(s) 441
SatI GCNGC 3 cut(s) 93, 294, 523
Sau3AI GATC 3 cut(s) 16, 153, 581
Sau96I GGNCC 2 cut(s) 94, 376
ScrFI CCNGG 1 cut(s) 164
SinI GGWCC 1 cut(s) 376
SmiMI CAYNNNNRTG 1 cut(s) 122
SphI GCATGC 1 cut(s) 414
Sse9I AATT 1 cut(s) 158
SsiI CCGC 2 cut(s) 92, 523
SspI AATATT 1 cut(s) 439
SspMI CTAG 1 cut(s) 380
StyD4I CCNGG 1 cut(s) 162
StyI CCWWGG 1 cut(s) 371
TaaI ACNGT 5 cut(s) 419, 531, 544, 578, 604
TaiI ACGT 4 cut(s) 49, 55, 84, 393
TaqII GACCGA 1 cut(s) 22
TasI AATT 1 cut(s) 158
TatI WGTACW 1 cut(s) 85
TauI GCSGC 2 cut(s) 95, 525
TfiI GAWTC 2 cut(s) 286, 474
Tru1I TTAA 1 cut(s) 441
Tru9I TTAA 1 cut(s) 441
TscAI CASTG 3 cut(s) 422, 534, 595
TseFI GTSAC 2 cut(s) 391, 544
TseI GCWGC 1 cut(s) 293
Tsp45I GTSAC 2 cut(s) 391, 544
TspDTI ATGAA 3 cut(s) 112, 136, 202
TspGWI ACGGA 1 cut(s) 325
TspRI CASTG 3 cut(s) 422, 534, 595
Van91I CCANNNNNTGG 1 cut(s) 372
VpaK11BI GGWCC 1 cut(s) 376
VspI ATTAAT 1 cut(s) 441
XapI RAATTY 1 cut(s) 158
XceI RCATGY 1 cut(s) 414
XmiI GTMKAC 1 cut(s) 499
XspI CTAG 1 cut(s) 380
Zsp2I ATGCAT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.