Rh7AG494100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000574
Physical Location & Seq
Reverse (-)
69454215 .. 69455293
1079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG494100.1

Sequence Viewer

Length: 561 bp
ATGGCCAGCCGTCCATTGACACCCTCGGTCCCTGAACGTGGTAATAATATCTTCATCTTCAACTTTGGACTAGCGGCCTCTTCCACCTTCGCCATTTTCACTTTCATGGTGCCGACACTTCTCACCTTCATCCAGACACTTCTCACCTTCATCCCATTTGAAACCCACCCCTTCAAGTTCATGGTTGCCATTCTCAGCTTCCTTGCTTTCTTTTGCTTTGCCATTATTGGAGCCACCCAAAACTTCTTCCCAAATCACGCTCCAAAACTTTTTATGGCTATCAAGTGTACGGCCTCGCTCTCGGTGGCTTTGCTTGTGTCGCTTTTGCTTCCACAGCCTTGGGACCAAGTGTCATATGTGACCTACTTCTTGTATGCATGCCACTGTCTCGGCTTACTCAGAAATATTAATCTGGCTTATTACCACGACATTGCTTATGGATTTGTGCGTAGCTCACTCGCCCGTCTACAGCAGTTGATACAAACGCGCCGCCCACCGTTGCCGCTGACAGTCAGAGATATCTCCAACCACCCGATTGCCAACCGTCGATCAATCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

186

Amino Acids

21.04

Weight (kDa)

9.8

Isoelectric Point (pI)

56.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 109
AccI GTMKAC 1 cut(s) 466
AccII CGCG 1 cut(s) 487
AciI CCGC 3 cut(s) 74, 490, 503
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 1 cut(s) 289
AfiI CCNNNNNNNGG 1 cut(s) 38
AgsI TTSAA 3 cut(s) 61, 161, 175
AhdI GACNNNNNGTC 1 cut(s) 349
AluBI AGCT 2 cut(s) 198, 453
AluI AGCT 2 cut(s) 198, 453
Alw26I GTCTC 1 cut(s) 392
AoxI GGCC 3 cut(s) 3, 75, 291
AseI ATTAAT 1 cut(s) 408
AspLEI GCGC 1 cut(s) 489
AspS9I GGNCC 2 cut(s) 28, 343
AsuHPI GGTGA 2 cut(s) 115, 136
AvaII GGWCC 2 cut(s) 28, 343
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 109
BceAI ACGGC 1 cut(s) 306
BcoDI GTCTC 1 cut(s) 392
BfaI CTAG 1 cut(s) 71
BfmI CTRYAG 1 cut(s) 467
BisI GCNGC 3 cut(s) 75, 490, 503
BlsI GCNGC 3 cut(s) 76, 491, 504
Bme18I GGWCC 2 cut(s) 28, 343
BmeRI GACNNNNNGTC 1 cut(s) 349
BmgT120I GGNCC 2 cut(s) 28, 343
BmiI GGNNCC 4 cut(s) 30, 111, 232, 344
BsaJI CCNNGG 2 cut(s) 24, 338
Bsc4I CCNNNNNNNGG 1 cut(s) 38
Bse3DI GCAATG 1 cut(s) 429
BseDI CCNNGG 2 cut(s) 24, 338
BseGI GGATG 2 cut(s) 129, 150
BseLI CCNNNNNNNGG 1 cut(s) 38
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 2 cut(s) 208, 412
Bsh1236I CGCG 1 cut(s) 487
BshFI GGCC 3 cut(s) 5, 77, 293
BshNI GGYRCC 1 cut(s) 109
BslFI GGGAC 2 cut(s) 14, 356
BslI CCNNNNNNNGG 1 cut(s) 38
BsmAI GTCTC 1 cut(s) 392
BsmFI GGGAC 2 cut(s) 14, 356
BsnI GGCC 3 cut(s) 5, 77, 293
Bsp143I GATC 1 cut(s) 548
BspACI CCGC 3 cut(s) 74, 490, 503
BspANI GGCC 3 cut(s) 5, 77, 293
BspCNI CTCAG 2 cut(s) 207, 411
BspFNI CGCG 1 cut(s) 487
BspLI GGNNCC 4 cut(s) 30, 111, 232, 344
BspT107I GGYRCC 1 cut(s) 109
BsrDI GCAATG 1 cut(s) 429
BssECI CCNNGG 2 cut(s) 24, 338
BssMI GATC 1 cut(s) 548
BssT1I CCWWGG 1 cut(s) 338
Bst4CI ACNGT 4 cut(s) 386, 498, 511, 545
Bst6I CTCTTC 1 cut(s) 85
BstC8I GCNNGC 2 cut(s) 7, 379
BstDEI CTNAG 2 cut(s) 194, 398
BstF5I GGATG 2 cut(s) 129, 150
BstFNI CGCG 1 cut(s) 487
BstHHI GCGC 1 cut(s) 489
BstKTI GATC 1 cut(s) 551
BstMAI GTCTC 1 cut(s) 392
BstMBI GATC 1 cut(s) 548
BstMWI GCNNNNNNNGC 2 cut(s) 319, 334
BstNSI RCATGY 1 cut(s) 381
BstSFI CTRYAG 1 cut(s) 467
BstUI CGCG 1 cut(s) 487
BstXI CCANNNNNNTGG 1 cut(s) 339
BsuRI GGCC 3 cut(s) 5, 77, 293
BtsCI GGATG 2 cut(s) 129, 150
BtsIMutI CAGTG 1 cut(s) 382
Cac8I GCNNGC 2 cut(s) 7, 379
CfoI GCGC 1 cut(s) 489
Cfr13I GGNCC 2 cut(s) 28, 343
Csp6I GTAC 1 cut(s) 288
CviAII CATG 3 cut(s) 106, 181, 378
CviQI GTAC 1 cut(s) 288
DdeI CTNAG 2 cut(s) 194, 398
DpnI GATC 1 cut(s) 550
DpnII GATC 1 cut(s) 548
DriI GACNNNNNGTC 1 cut(s) 349
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 85
Eam1105I GACNNNNNGTC 1 cut(s) 349
EarI CTCTTC 1 cut(s) 85
Eco130I CCWWGG 1 cut(s) 338
Eco32I GATATC 1 cut(s) 520
Eco47I GGWCC 2 cut(s) 28, 343
EcoRV GATATC 1 cut(s) 520
EcoT14I CCWWGG 1 cut(s) 338
EcoT22I ATGCAT 1 cut(s) 379
ErhI CCWWGG 1 cut(s) 338
FaeI CATG 3 cut(s) 109, 184, 381
FaiI YATR 8 cut(s) 107, 182, 275, 355, 357, 375, 379, 438
FaqI GGGAC 2 cut(s) 14, 356
FatI CATG 3 cut(s) 105, 180, 377
FauNDI CATATG 1 cut(s) 355
FblI GTMKAC 1 cut(s) 466
Fnu4HI GCNGC 3 cut(s) 75, 490, 503
FokI GGATG 2 cut(s) 116, 137
Fsp4HI GCNGC 3 cut(s) 75, 490, 503
FspBI CTAG 1 cut(s) 71
GlaI GCGC 1 cut(s) 488
GluI GCNGC 3 cut(s) 75, 490, 503
HaeIII GGCC 3 cut(s) 5, 77, 293
HhaI GCGC 1 cut(s) 489
Hin1II CATG 3 cut(s) 109, 184, 381
Hin6I GCGC 1 cut(s) 487
HinP1I GCGC 1 cut(s) 487
HphI GGTGA 2 cut(s) 115, 136
Hpy166II GTNNAC 2 cut(s) 288, 467
Hpy188I TCNGA 2 cut(s) 401, 515
Hpy188III TCNNGA 1 cut(s) 133
Hpy8I GTNNAC 2 cut(s) 288, 467
Hpy99I CGWCG 1 cut(s) 549
HpyAV CCTTC 4 cut(s) 97, 136, 157, 181
HpyCH4III ACNGT 4 cut(s) 386, 498, 511, 545
HpyCH4IV ACGT 1 cut(s) 37
HpyCH4V TGCA 1 cut(s) 377
HpyF10VI GCNNNNNNNGC 2 cut(s) 319, 334
HpyF3I CTNAG 2 cut(s) 194, 398
HpySE526I ACGT 1 cut(s) 37
Hsp92II CATG 3 cut(s) 109, 184, 381
HspAI GCGC 1 cut(s) 487
Kzo9I GATC 1 cut(s) 548
LmnI GCTCC 2 cut(s) 230, 265
LpnPI CCDG 4 cut(s) 19, 45, 146, 398
MaeI CTAG 1 cut(s) 71
MaeII ACGT 1 cut(s) 37
MaeIII GTNAC 1 cut(s) 358
MalI GATC 1 cut(s) 550
MboI GATC 1 cut(s) 548
MboII GAAGA 4 cut(s) 43, 49, 72, 238
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 549
MnlI CCTC 3 cut(s) 34, 88, 304
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 379
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 408
MslI CAYNNNNRTG 1 cut(s) 104
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 505
MvnI CGCG 1 cut(s) 487
MwoI GCNNNNNNNGC 2 cut(s) 319, 334
NdeI CATATG 1 cut(s) 355
NdeII GATC 1 cut(s) 548
NlaIII CATG 3 cut(s) 109, 184, 381
NlaIV GGNNCC 4 cut(s) 30, 111, 232, 344
NmeAIII GCCGAG 1 cut(s) 369
NmuCI GTSAC 1 cut(s) 358
NsiI ATGCAT 1 cut(s) 379
NspI RCATGY 1 cut(s) 381
PaeI GCATGC 1 cut(s) 381
PkrI GCNGC 3 cut(s) 76, 491, 504
PshBI ATTAAT 1 cut(s) 408
PspN4I GGNNCC 4 cut(s) 30, 111, 232, 344
PspPI GGNCC 2 cut(s) 28, 343
RsaI GTAC 1 cut(s) 289
RsaNI GTAC 1 cut(s) 288
RseI CAYNNNNRTG 1 cut(s) 104
SaqAI TTAA 1 cut(s) 408
SatI GCNGC 3 cut(s) 75, 490, 503
Sau3AI GATC 1 cut(s) 548
Sau96I GGNCC 2 cut(s) 28, 343
SetI ASST 7 cut(s) 40, 89, 128, 149, 200, 365, 455
SfcI CTRYAG 1 cut(s) 467
SinI GGWCC 2 cut(s) 28, 343
SmiMI CAYNNNNRTG 1 cut(s) 104
SphI GCATGC 1 cut(s) 381
SsiI CCGC 3 cut(s) 74, 490, 503
SspI AATATT 1 cut(s) 406
SspMI CTAG 1 cut(s) 71
StyI CCWWGG 1 cut(s) 338
TaaI ACNGT 4 cut(s) 386, 498, 511, 545
TaiI ACGT 1 cut(s) 40
TaqI TCGA 1 cut(s) 547
TaqII GACCGA 1 cut(s) 16
TauI GCSGC 3 cut(s) 77, 492, 505
Tru1I TTAA 1 cut(s) 408
Tru9I TTAA 1 cut(s) 408
TscAI CASTG 1 cut(s) 389
TseFI GTSAC 1 cut(s) 358
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 5 cut(s) 43, 94, 118, 139, 169
TspRI CASTG 1 cut(s) 389
VpaK11BI GGWCC 2 cut(s) 28, 343
VspI ATTAAT 1 cut(s) 408
XceI RCATGY 1 cut(s) 381
XmiI GTMKAC 1 cut(s) 466
XspI CTAG 1 cut(s) 71
Zsp2I ATGCAT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.