Rh1AG223300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000574
Physical Location & Seq
Forward (+)
42516484 .. 42517008
525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG223300.1

Sequence Viewer

Length: 525 bp
ATGGCAAGCCATCAGTTTTTTTGTGTTGGGCTGATGATCAACAATCCTCATTTCAATCACGTTGACATGTACTTGATCAGTAACTACATGCTGTTCATGGACTTTGCTCTTCAAAACCATCATGAAGGTGAGGTTGTTGTGGGCTTCGCCATTCCCATACTGCTAACCTTGGTTCAGATCAGATATCCAGCAGAAGATTTGTTCCGAACACGCCCTATAACCATCACGGTTGTCCTGTCTAGCTTGCTTTTCTATTGCCTGGCCCTCGCCTTCGGGGTACTAATTAGGCAAAGACGTGGCCATGCTCATTTTCATGGTCATGTTCATAGTTATTCTCCTCGTAGTTATTGGTATCGGATTGCCTTGACGGTGTTTGGTTCAATTTCAGTAGCTTCACTCCTTGCACGTCTGTTTCCACACAACAACTCCACTTCACCGCTGCGTCCTCTGCTCTTCGTTTTCTTCGTCTTCGTGTTGCTGGGGAGCTTGGTGCCTAGAAATTGTACAGGTGAAATCGGGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

174

Amino Acids

19.81

Weight (kDa)

9.25

Isoelectric Point (pI)

36.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 490
AciI CCGC 1 cut(s) 437
AcoI YGGCCR 1 cut(s) 298
AfaI GTAC 3 cut(s) 71, 279, 505
AflIII ACRYGT 1 cut(s) 66
AgsI TTSAA 3 cut(s) 55, 113, 381
AjiI CACGTC 2 cut(s) 296, 407
AjnI CCWGG 1 cut(s) 258
AluBI AGCT 3 cut(s) 243, 392, 486
AluI AGCT 3 cut(s) 243, 392, 486
AoxI GGCC 2 cut(s) 261, 298
ApeKI GCWGC 1 cut(s) 439
AspS9I GGNCC 1 cut(s) 262
AsuHPI GGTGA 3 cut(s) 140, 426, 521
BalI TGGCCA 1 cut(s) 300
BanI GGYRCC 1 cut(s) 490
BbsI GAAGAC 1 cut(s) 460
BbvI GCAGC 1 cut(s) 426
BccI CCATC 3 cut(s) 18, 126, 230
BciT130I CCWGG 1 cut(s) 260
BclI TGATCA 2 cut(s) 36, 75
BfaI CTAG 2 cut(s) 240, 495
BisI GCNGC 1 cut(s) 440
BlsI GCNGC 1 cut(s) 441
Bme1390I CCNGG 1 cut(s) 260
BmgBI CACGTC 2 cut(s) 296, 407
BmgT120I GGNCC 1 cut(s) 262
BmiI GGNNCC 1 cut(s) 492
BmrFI CCNGG 1 cut(s) 260
BpiI GAAGAC 1 cut(s) 460
BsaJI CCNNGG 1 cut(s) 168
BsaXI ACNNNNNCTCC 2 cut(s) 410, 440
BseBI CCWGG 1 cut(s) 260
BseDI CCNNGG 1 cut(s) 168
BseRI GAGGAG 1 cut(s) 327
BseXI GCAGC 1 cut(s) 426
BseYI CCCAGC 1 cut(s) 478
BshFI GGCC 2 cut(s) 263, 300
BshNI GGYRCC 1 cut(s) 490
BsnI GGCC 2 cut(s) 263, 300
Bsp1407I TGTACA 1 cut(s) 503
Bsp143I GATC 3 cut(s) 36, 75, 177
BspACI CCGC 1 cut(s) 437
BspANI GGCC 2 cut(s) 263, 300
BspHI TCATGA 2 cut(s) 121, 521
BspLI GGNNCC 1 cut(s) 492
BspQI GCTCTTC 2 cut(s) 114, 458
BspT107I GGYRCC 1 cut(s) 490
BsrGI TGTACA 1 cut(s) 503
BssECI CCNNGG 1 cut(s) 168
BssMI GATC 3 cut(s) 36, 75, 177
BssT1I CCWWGG 1 cut(s) 168
Bst2UI CCWGG 1 cut(s) 260
Bst4CI ACNGT 2 cut(s) 229, 370
Bst6I CTCTTC 2 cut(s) 114, 458
BstAUI TGTACA 1 cut(s) 503
BstC8I GCNNGC 2 cut(s) 7, 245
BstKTI GATC 3 cut(s) 39, 78, 180
BstMBI GATC 3 cut(s) 36, 75, 177
BstMWI GCNNNNNNNGC 1 cut(s) 448
BstNI CCWGG 1 cut(s) 260
BstNSI RCATGY 2 cut(s) 70, 91
BstSCI CCNGG 1 cut(s) 258
BstV1I GCAGC 1 cut(s) 426
BstV2I GAAGAC 1 cut(s) 460
BsuRI GGCC 2 cut(s) 263, 300
BtrI CACGTC 2 cut(s) 296, 407
Cac8I GCNNGC 2 cut(s) 7, 245
CciI TCATGA 2 cut(s) 121, 521
Cfr13I GGNCC 1 cut(s) 262
CseI GACGC 1 cut(s) 431
Csp6I GTAC 3 cut(s) 70, 278, 504
CviAII CATG 8 cut(s) 67, 88, 97, 122, 302, 314, 320, 522
CviJI RGCY 8 cut(s) 9, 31, 144, 243, 263, 300, 392, 486
CviKI_1 RGCY 8 cut(s) 9, 31, 144, 243, 263, 300, 392, 486
CviQI GTAC 3 cut(s) 70, 278, 504
DpnI GATC 3 cut(s) 38, 77, 179
DpnII GATC 3 cut(s) 36, 75, 177
EaeI YGGCCR 1 cut(s) 298
Eam1104I CTCTTC 2 cut(s) 114, 458
EarI CTCTTC 2 cut(s) 114, 458
Eco130I CCWWGG 1 cut(s) 168
Eco32I GATATC 1 cut(s) 185
EcoRII CCWGG 1 cut(s) 258
EcoRV GATATC 1 cut(s) 185
EcoT14I CCWWGG 1 cut(s) 168
ErhI CCWWGG 1 cut(s) 168
FaeI CATG 8 cut(s) 70, 91, 100, 125, 305, 317, 323, 525
FatI CATG 8 cut(s) 66, 87, 96, 121, 301, 313, 319, 521
FbaI TGATCA 2 cut(s) 36, 75
Fnu4HI GCNGC 1 cut(s) 440
Fsp4HI GCNGC 1 cut(s) 440
FspBI CTAG 2 cut(s) 240, 495
GluI GCNGC 1 cut(s) 440
GsaI CCCAGC 1 cut(s) 482
HaeIII GGCC 2 cut(s) 263, 300
HgaI GACGC 1 cut(s) 431
Hin1II CATG 8 cut(s) 70, 91, 100, 125, 305, 317, 323, 525
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HphI GGTGA 3 cut(s) 140, 426, 521
Hpy166II GTNNAC 1 cut(s) 64
Hpy188I TCNGA 4 cut(s) 177, 182, 206, 357
Hpy188III TCNNGA 2 cut(s) 122, 522
Hpy8I GTNNAC 1 cut(s) 64
HpyAV CCTTC 2 cut(s) 119, 280
HpyCH4III ACNGT 2 cut(s) 229, 370
HpyCH4IV ACGT 3 cut(s) 60, 295, 406
HpyCH4V TGCA 1 cut(s) 404
HpyF10VI GCNNNNNNNGC 1 cut(s) 448
HpySE526I ACGT 3 cut(s) 60, 295, 406
Hsp92II CATG 8 cut(s) 70, 91, 100, 125, 305, 317, 323, 525
Ksp22I TGATCA 2 cut(s) 36, 75
Kzo9I GATC 3 cut(s) 36, 75, 177
LguI GCTCTTC 2 cut(s) 114, 458
LmnI GCTCC 1 cut(s) 483
LpnPI CCDG 6 cut(s) 201, 245, 248, 272, 464, 492
Lsp1109I GCAGC 1 cut(s) 426
MaeI CTAG 2 cut(s) 240, 495
MaeII ACGT 3 cut(s) 60, 295, 406
MaeIII GTNAC 1 cut(s) 80
MalI GATC 3 cut(s) 38, 77, 179
MboI GATC 3 cut(s) 36, 75, 177
MboII GAAGA 5 cut(s) 101, 206, 445, 454, 460
MlsI TGGCCA 1 cut(s) 300
MluCI AATT 3 cut(s) 282, 381, 499
MluNI TGGCCA 1 cut(s) 300
MnlI CCTC 5 cut(s) 57, 124, 275, 348, 456
Mox20I TGGCCA 1 cut(s) 300
MscI TGGCCA 1 cut(s) 300
MslI CAYNNNNRTG 3 cut(s) 126, 312, 318
Msp20I TGGCCA 1 cut(s) 300
MspA1I CMGCKG 1 cut(s) 439
MspR9I CCNGG 1 cut(s) 260
MvaI CCWGG 1 cut(s) 260
MwoI GCNNNNNNNGC 1 cut(s) 448
NdeII GATC 3 cut(s) 36, 75, 177
NlaIII CATG 8 cut(s) 70, 91, 100, 125, 305, 317, 323, 525
NlaIV GGNNCC 1 cut(s) 492
NspI RCATGY 2 cut(s) 70, 91
PagI TCATGA 2 cut(s) 121, 521
PciI ACATGT 1 cut(s) 66
PciSI GCTCTTC 2 cut(s) 114, 458
PcsI WCGNNNNNNNCGW 1 cut(s) 462
PkrI GCNGC 1 cut(s) 441
PscI ACATGT 1 cut(s) 66
Psp6I CCWGG 1 cut(s) 258
PspFI CCCAGC 1 cut(s) 478
PspGI CCWGG 1 cut(s) 258
PspN4I GGNNCC 1 cut(s) 492
PspPI GGNCC 1 cut(s) 262
RsaI GTAC 3 cut(s) 71, 279, 505
RsaNI GTAC 3 cut(s) 70, 278, 504
RseI CAYNNNNRTG 3 cut(s) 126, 312, 318
SapI GCTCTTC 2 cut(s) 114, 458
SatI GCNGC 1 cut(s) 440
Sau3AI GATC 3 cut(s) 36, 75, 177
Sau96I GGNCC 1 cut(s) 262
ScrFI CCNGG 1 cut(s) 260
SmiMI CAYNNNNRTG 3 cut(s) 126, 312, 318
Sse9I AATT 3 cut(s) 282, 381, 499
SsiI CCGC 1 cut(s) 437
SspMI CTAG 2 cut(s) 240, 495
StyD4I CCNGG 1 cut(s) 258
StyI CCWWGG 1 cut(s) 168
TaaI ACNGT 2 cut(s) 229, 370
TaiI ACGT 3 cut(s) 63, 298, 409
TasI AATT 3 cut(s) 282, 381, 499
TatI WGTACW 2 cut(s) 69, 503
TseI GCWGC 1 cut(s) 439
TspDTI ATGAA 4 cut(s) 85, 138, 302, 314
XceI RCATGY 2 cut(s) 70, 91
XspI CTAG 2 cut(s) 240, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.