Rh1AG275200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000574
Physical Location & Seq
Forward (+)
50334284 .. 50334832
549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG275200.1

Sequence Viewer

Length: 549 bp
ATGGCCGATCTTGAGTATCAAATGCGCCCAGCCCTTATCGTCCACGAACATAATTCCTTTACTATCACCTTCAACATTCTTCTAATCAATTTCGCAGCTTCCACTTCTACTCATGCCATTCTCGCTTTCCTCGTCCCCATACTCGGCGCCTTTATTCAAATCAAGTTCCCAGGAATTTCAGTACTGTCTTCTCCATTCGAAACACATCGTAACACCACCGTCGTCGCGATTGCAAGCTTACTTGCATATTGCTTGATTGTTGGGGTTAGGGTAAGGTTTCCAGCTAGGGCTCGTACTTGGTCTAGATTTGCCCTTTTGTTTTCTGGTTTGCTCTCCGTTGCTCTTGCTTCACTGCTTTCAATTTTGATCCCAGATTCTTGGAACCATGTGCCATATATGATGTACTTCATTTATGTCATAGGGGAGGGTTTCCAATTAGTCCGAATGCTTTGTCGACAAGCGGGCCGACGTCAAATGAGATCGAGGCGCACACTGCTCCCTTTGAGGGTAGAGCTAGCGGAGATTCATGAGTTTGCAGCACGTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

182

Amino Acids

20.56

Weight (kDa)

10.5

Isoelectric Point (pI)

47.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 472
AccB1I GGYRCC 1 cut(s) 146
AccI GTMKAC 1 cut(s) 454
AccII CGCG 1 cut(s) 227
AciI CCGC 2 cut(s) 461, 518
AclWI GGATC 1 cut(s) 361
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 174
AcvI CACGTG 1 cut(s) 542
AcyI GRCGYC 2 cut(s) 147, 469
AfaI GTAC 3 cut(s) 183, 295, 404
AfiI CCNNNNNNNGG 2 cut(s) 143, 505
AgsI TTSAA 3 cut(s) 73, 158, 360
AjnI CCWGG 1 cut(s) 169
AluBI AGCT 4 cut(s) 98, 237, 284, 514
AluI AGCT 4 cut(s) 98, 237, 284, 514
AlwI GGATC 1 cut(s) 361
AoxI GGCC 2 cut(s) 3, 463
ApeKI GCWGC 2 cut(s) 95, 536
ApoI RAATTY 1 cut(s) 174
ArsI GACNNNNNNTTYG 2 cut(s) 436, 468
AspLEI GCGC 3 cut(s) 27, 149, 489
AspS9I GGNCC 1 cut(s) 463
AsuHPI GGTGA 1 cut(s) 58
AsuII TTCGAA 1 cut(s) 198
AsuNHI GCTAGC 1 cut(s) 514
BanI GGYRCC 1 cut(s) 146
BanII GRGCYC 1 cut(s) 292
BbrPI CACGTG 1 cut(s) 542
BbsI GAAGAC 1 cut(s) 180
BbvI GCAGC 1 cut(s) 107
BciT130I CCWGG 1 cut(s) 171
BfaI CTAG 3 cut(s) 285, 303, 515
BfoI RGCGCY 1 cut(s) 150
BisI GCNGC 2 cut(s) 96, 537
BlsI GCNGC 2 cut(s) 97, 538
BmcAI AGTACT 1 cut(s) 183
Bme1390I CCNGG 1 cut(s) 171
BmgT120I GGNCC 1 cut(s) 463
BmiI GGNNCC 2 cut(s) 148, 383
BmrFI CCNGG 1 cut(s) 171
BmtI GCTAGC 1 cut(s) 518
BpiI GAAGAC 1 cut(s) 180
Bpu14I TTCGAA 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 32
BsaAI YACGTR 1 cut(s) 542
BsaHI GRCGYC 2 cut(s) 147, 469
BsaJI CCNNGG 1 cut(s) 169
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 505
BseBI CCWGG 1 cut(s) 171
BseDI CCNNGG 1 cut(s) 169
BseLI CCNNNNNNNGG 2 cut(s) 143, 505
BseXI GCAGC 1 cut(s) 107
BseYI CCCAGC 1 cut(s) 28
Bsh1236I CGCG 1 cut(s) 227
BshFI GGCC 2 cut(s) 5, 465
BshNI GGYRCC 1 cut(s) 146
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 2 cut(s) 143, 505
BsmFI GGGAC 1 cut(s) 119
BsmI GAATGC 1 cut(s) 450
BsnI GGCC 2 cut(s) 5, 465
Bsp119I TTCGAA 1 cut(s) 198
Bsp1286I GDGCHC 1 cut(s) 292
Bsp143I GATC 3 cut(s) 7, 366, 479
Bsp68I TCGCGA 1 cut(s) 227
BspACI CCGC 2 cut(s) 461, 518
BspANI GGCC 2 cut(s) 5, 465
BspFNI CGCG 1 cut(s) 227
BspHI TCATGA 1 cut(s) 526
BspLI GGNNCC 2 cut(s) 148, 383
BspOI GCTAGC 1 cut(s) 518
BspPI GGATC 1 cut(s) 361
BspT104I TTCGAA 1 cut(s) 198
BspT107I GGYRCC 1 cut(s) 146
BssECI CCNNGG 1 cut(s) 169
BssMI GATC 3 cut(s) 7, 366, 479
BssNI GRCGYC 2 cut(s) 147, 469
Bst2UI CCWGG 1 cut(s) 171
Bst4CI ACNGT 2 cut(s) 186, 220
BstACI GRCGYC 2 cut(s) 147, 469
BstAPI GCANNNNNTGC 1 cut(s) 542
BstBAI YACGTR 1 cut(s) 542
BstBI TTCGAA 1 cut(s) 198
BstC8I GCNNGC 3 cut(s) 235, 463, 516
BstFNI CGCG 1 cut(s) 227
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 3 cut(s) 27, 149, 489
BstKTI GATC 3 cut(s) 10, 369, 482
BstMBI GATC 3 cut(s) 7, 366, 479
BstMWI GCNNNNNNNGC 3 cut(s) 122, 493, 542
BstNI CCWGG 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 169
BstUI CGCG 1 cut(s) 227
BstV1I GCAGC 1 cut(s) 107
BstV2I GAAGAC 1 cut(s) 180
BstXI CCANNNNNNTGG 1 cut(s) 378
BsuRI GGCC 2 cut(s) 5, 465
BtsI GCAGTG 2 cut(s) 350, 491
BtsIMutI CAGTG 2 cut(s) 350, 491
BtuMI TCGCGA 1 cut(s) 227
Cac8I GCNNGC 3 cut(s) 235, 463, 516
CciI TCATGA 1 cut(s) 526
CfoI GCGC 3 cut(s) 27, 149, 489
Cfr13I GGNCC 1 cut(s) 463
Csp6I GTAC 3 cut(s) 182, 294, 403
CviAII CATG 4 cut(s) 113, 386, 527, 546
CviJI RGCY 8 cut(s) 5, 32, 98, 237, 284, 290, 465, 514
CviKI_1 RGCY 8 cut(s) 5, 32, 98, 237, 284, 290, 465, 514
CviQI GTAC 3 cut(s) 182, 294, 403
DinI GGCGCC 1 cut(s) 148
DpnI GATC 3 cut(s) 9, 368, 481
DpnII GATC 3 cut(s) 7, 366, 479
EaeI YGGCCR 1 cut(s) 3
Eco24I GRGCYC 1 cut(s) 292
Eco72I CACGTG 1 cut(s) 542
EcoRII CCWGG 1 cut(s) 169
EcoT38I GRGCYC 1 cut(s) 292
EgeI GGCGCC 1 cut(s) 148
EheI GGCGCC 1 cut(s) 148
FaeI CATG 4 cut(s) 116, 389, 530, 549
FaqI GGGAC 1 cut(s) 119
FatI CATG 4 cut(s) 112, 385, 526, 545
FauI CCCGC 1 cut(s) 454
FblI GTMKAC 1 cut(s) 454
Fnu4HI GCNGC 2 cut(s) 96, 537
FriOI GRGCYC 1 cut(s) 292
Fsp4HI GCNGC 2 cut(s) 96, 537
FspBI CTAG 3 cut(s) 285, 303, 515
GlaI GCGC 3 cut(s) 26, 148, 488
GluI GCNGC 2 cut(s) 96, 537
GsaI CCCAGC 1 cut(s) 32
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 2 cut(s) 5, 465
HhaI GCGC 3 cut(s) 27, 149, 489
Hin1I GRCGYC 2 cut(s) 147, 469
Hin1II CATG 4 cut(s) 116, 389, 530, 549
Hin6I GCGC 3 cut(s) 25, 147, 487
HinP1I GCGC 3 cut(s) 25, 147, 487
HincII GTYRAC 1 cut(s) 455
HindII GTYRAC 1 cut(s) 455
HindIII AAGCTT 1 cut(s) 235
HinfI GANTC 2 cut(s) 374, 523
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 2 cut(s) 43, 455
Hpy188I TCNGA 1 cut(s) 443
Hpy188III TCNNGA 4 cut(s) 11, 226, 303, 527
Hpy8I GTNNAC 2 cut(s) 43, 455
Hpy99I CGWCG 3 cut(s) 224, 227, 471
HpyAV CCTTC 1 cut(s) 79
HpyCH4III ACNGT 2 cut(s) 186, 220
HpyCH4IV ACGT 2 cut(s) 469, 541
HpyCH4V TGCA 4 cut(s) 233, 245, 536, 545
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 493, 542
HpySE526I ACGT 2 cut(s) 469, 541
Hsp92I GRCGYC 2 cut(s) 147, 469
Hsp92II CATG 4 cut(s) 116, 389, 530, 549
HspAI GCGC 3 cut(s) 25, 147, 487
KasI GGCGCC 1 cut(s) 146
Kzo9I GATC 3 cut(s) 7, 366, 479
LmnI GCTCC 1 cut(s) 501
LpnPI CCDG 6 cut(s) 42, 156, 183, 294, 309, 384
Lsp1109I GCAGC 1 cut(s) 107
MaeI CTAG 3 cut(s) 285, 303, 515
MaeII ACGT 2 cut(s) 469, 541
MaeIII GTNAC 1 cut(s) 209
MalI GATC 3 cut(s) 9, 368, 481
MboI GATC 3 cut(s) 7, 366, 479
MboII GAAGA 2 cut(s) 71, 180
MhlI GDGCHC 1 cut(s) 292
MluCI AATT 5 cut(s) 52, 88, 174, 360, 434
Mly113I GGCGCC 1 cut(s) 147
MnlI CCTC 4 cut(s) 140, 418, 477, 498
MslI CAYNNNNRTG 1 cut(s) 544
MspR9I CCNGG 1 cut(s) 171
Mva1269I GAATGC 1 cut(s) 450
MvaI CCWGG 1 cut(s) 171
MvnI CGCG 1 cut(s) 227
MwoI GCNNNNNNNGC 3 cut(s) 122, 493, 542
NarI GGCGCC 1 cut(s) 147
NdeII GATC 3 cut(s) 7, 366, 479
NheI GCTAGC 1 cut(s) 514
NlaIII CATG 4 cut(s) 116, 389, 530, 549
NlaIV GGNNCC 2 cut(s) 148, 383
NmeAIII GCCGAG 1 cut(s) 123
NruI TCGCGA 1 cut(s) 227
NspV TTCGAA 1 cut(s) 198
PagI TCATGA 1 cut(s) 526
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PctI GAATGC 1 cut(s) 450
PfeI GAWTC 2 cut(s) 374, 523
PkrI GCNGC 2 cut(s) 97, 538
PluTI GGCGCC 1 cut(s) 150
PmaCI CACGTG 1 cut(s) 542
PmlI CACGTG 1 cut(s) 542
Ppu21I YACGTR 1 cut(s) 542
Psp6I CCWGG 1 cut(s) 169
PspCI CACGTG 1 cut(s) 542
PspFI CCCAGC 1 cut(s) 28
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 2 cut(s) 148, 383
PspPI GGNCC 1 cut(s) 463
RruI TCGCGA 1 cut(s) 227
RsaI GTAC 3 cut(s) 183, 295, 404
RsaNI GTAC 3 cut(s) 182, 294, 403
RseI CAYNNNNRTG 1 cut(s) 544
SalI GTCGAC 1 cut(s) 453
SatI GCNGC 2 cut(s) 96, 537
Sau3AI GATC 3 cut(s) 7, 366, 479
Sau96I GGNCC 1 cut(s) 463
ScaI AGTACT 1 cut(s) 183
ScrFI CCNGG 1 cut(s) 171
SduI GDGCHC 1 cut(s) 292
SetI ASST 8 cut(s) 71, 100, 239, 278, 286, 472, 516, 544
SfoI GGCGCC 1 cut(s) 148
SfuI TTCGAA 1 cut(s) 198
SmiMI CAYNNNNRTG 1 cut(s) 544
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
Sse9I AATT 5 cut(s) 52, 88, 174, 360, 434
SsiI CCGC 2 cut(s) 461, 518
SspDI GGCGCC 1 cut(s) 146
SspMI CTAG 3 cut(s) 285, 303, 515
StyD4I CCNGG 1 cut(s) 169
TaaI ACNGT 2 cut(s) 186, 220
TaiI ACGT 2 cut(s) 472, 544
TaqI TCGA 3 cut(s) 198, 454, 482
TasI AATT 5 cut(s) 52, 88, 174, 360, 434
TatI WGTACW 2 cut(s) 181, 402
TfiI GAWTC 2 cut(s) 374, 523
TscAI CASTG 2 cut(s) 357, 498
TseI GCWGC 2 cut(s) 95, 536
TspDTI ATGAA 2 cut(s) 397, 515
TspGWI ACGGA 1 cut(s) 325
TspRI CASTG 2 cut(s) 357, 498
XapI RAATTY 1 cut(s) 174
XbaI TCTAGA 1 cut(s) 302
XmiI GTMKAC 1 cut(s) 454
XspI CTAG 3 cut(s) 285, 303, 515
ZraI GACGTC 1 cut(s) 470
ZrmI AGTACT 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.