RLG00000035999

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000574
Physical Location & Seq
Reverse (-)
74734406 .. 74735975
1570 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035999

Sequence Viewer

Length: 591 bp
ATGGCCGAAGAGATGCCCAAGAAACAGCCAGCAGAGCTCAATTCCCCCTGTTATGCACTTGGACGTATTGTGCTTCTATTCAAGGACCAAGGCTTCGCTACTCTTTCCTCATTTGCGAAGTTTCGTTACAGCAAATCATCAAACAGGTCTCTCCCACCCCATCCTACAACACGAATATCAACCTTCATGCACACTTCCACTTCCACCAGAGCCGATCAGCATTGGTGCCGGCCAGAATTCAACACTCTGGTAATGATCAACCACGGACAAATGTCAACTCGTCCTTCCACTTCTACTTCAACTTCCGCTCATGCCATTTTCGCTTTCATTGTTGCCGTACTTGTTTCCTTCATCCAAATCAGATTCCCAGGAACAGTAATCTCCTCCCCTTTTGAAACACATCCTACGACTATCGTGGTTTTCATTGCTAGCTTATTTTCATACTGCTCGTCTCTTTTGGCCGCCAAAAGATCACCTGGTTATGCACCCACGTTTTGCATTGCCATGAGATCTTCTGGTTTACTTGCACTTGCTTCACTGCTCTCACTTCTGTTACCCGACTCTTATCACCGCGTGCCATACTGGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

21.75

Weight (kDa)

9.78

Isoelectric Point (pI)

52.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 225
AccBSI CCGCTC 1 cut(s) 308
AccII CGCG 1 cut(s) 573
AciI CCGC 3 cut(s) 306, 462, 571
AcoI YGGCCR 3 cut(s) 3, 230, 459
AcsI RAATTY 1 cut(s) 236
AdeI CACNNNGTG 1 cut(s) 574
AfaI GTAC 1 cut(s) 339
AgsI TTSAA 4 cut(s) 82, 241, 300, 395
AjnI CCWGG 2 cut(s) 367, 475
AluBI AGCT 2 cut(s) 37, 432
AluI AGCT 2 cut(s) 37, 432
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 2 cut(s) 153, 456
AoxI GGCC 3 cut(s) 3, 230, 459
ApoI RAATTY 1 cut(s) 236
ArsI GACNNNNNNTTYG 2 cut(s) 77, 109
AspS9I GGNCC 1 cut(s) 85
AsuHPI GGTGA 2 cut(s) 465, 560
AsuNHI GCTAGC 1 cut(s) 428
AvaII GGWCC 1 cut(s) 85
BanI GGYRCC 1 cut(s) 225
BanII GRGCYC 1 cut(s) 39
BarI GAAGNNNNNNTAC 2 cut(s) 110, 142
Bbv12I GWGCWC 1 cut(s) 39
BccI CCATC 1 cut(s) 168
BceAI ACGGC 1 cut(s) 320
BciT130I CCWGG 2 cut(s) 369, 477
BclI TGATCA 1 cut(s) 255
BcoDI GTCTC 2 cut(s) 153, 456
BfaI CTAG 1 cut(s) 429
BglII AGATCT 1 cut(s) 509
BisI GCNGC 1 cut(s) 462
BlsI GCNGC 1 cut(s) 463
Bme1390I CCNGG 2 cut(s) 369, 477
Bme18I GGWCC 1 cut(s) 85
BmgT120I GGNCC 1 cut(s) 85
BmiI GGNNCC 1 cut(s) 227
BmrFI CCNGG 2 cut(s) 369, 477
BmsI GCATC 1 cut(s) 3
BmtI GCTAGC 1 cut(s) 432
BoxI GACNNNNGTC 1 cut(s) 271
BsaI GGTCTC 1 cut(s) 153
BsaJI CCNNGG 3 cut(s) 88, 262, 367
Bse118I RCCGGY 1 cut(s) 228
Bse1I ACTGG 1 cut(s) 587
Bse3DI GCAATG 2 cut(s) 423, 498
BseBI CCWGG 2 cut(s) 369, 477
BseDI CCNNGG 3 cut(s) 88, 262, 367
BseGI GGATG 4 cut(s) 160, 351, 400, 591
BseMI GCAATG 2 cut(s) 423, 498
BseNI ACTGG 1 cut(s) 587
BseRI GAGGAG 1 cut(s) 373
Bsh1236I CGCG 1 cut(s) 573
BshFI GGCC 3 cut(s) 5, 232, 461
BshNI GGYRCC 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 39
BsiSI CCGG 1 cut(s) 229
BsmAI GTCTC 2 cut(s) 153, 456
BsmBI CGTCTC 1 cut(s) 456
BsnI GGCC 3 cut(s) 5, 232, 461
Bso31I GGTCTC 1 cut(s) 153
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 4 cut(s) 214, 255, 470, 509
BspACI CCGC 3 cut(s) 306, 462, 571
BspANI GGCC 3 cut(s) 5, 232, 461
BspFNI CGCG 1 cut(s) 573
BspLI GGNNCC 1 cut(s) 227
BspOI GCTAGC 1 cut(s) 432
BspT107I GGYRCC 1 cut(s) 225
BspTNI GGTCTC 1 cut(s) 153
BsrBI CCGCTC 1 cut(s) 308
BsrDI GCAATG 2 cut(s) 423, 498
BsrFI RCCGGY 1 cut(s) 228
BsrI ACTGG 1 cut(s) 587
BssAI RCCGGY 1 cut(s) 228
BssECI CCNNGG 3 cut(s) 88, 262, 367
BssMI GATC 4 cut(s) 214, 255, 470, 509
BssT1I CCWWGG 1 cut(s) 88
Bst2UI CCWGG 2 cut(s) 369, 477
Bst4CI ACNGT 1 cut(s) 376
Bst6I CTCTTC 1 cut(s) 3
BstC8I GCNNGC 4 cut(s) 30, 230, 430, 575
BstDSI CCRYGG 1 cut(s) 262
BstF5I GGATG 4 cut(s) 160, 351, 400, 591
BstFNI CGCG 1 cut(s) 573
BstKTI GATC 4 cut(s) 217, 258, 473, 512
BstMAI GTCTC 2 cut(s) 153, 456
BstMBI GATC 4 cut(s) 214, 255, 470, 509
BstMWI GCNNNNNNNGC 2 cut(s) 34, 320
BstNI CCWGG 2 cut(s) 369, 477
BstPAI GACNNNNGTC 1 cut(s) 271
BstSCI CCNGG 2 cut(s) 367, 475
BstUI CGCG 1 cut(s) 573
BstX2I RGATCY 1 cut(s) 509
BstYI RGATCY 1 cut(s) 509
BsuRI GGCC 3 cut(s) 5, 232, 461
BtgI CCRYGG 1 cut(s) 262
BtsCI GGATG 4 cut(s) 160, 351, 400, 591
BtsI GCAGTG 1 cut(s) 536
BtsIMutI CAGTG 1 cut(s) 536
Cac8I GCNNGC 4 cut(s) 30, 230, 430, 575
Cfr10I RCCGGY 1 cut(s) 228
Cfr13I GGNCC 1 cut(s) 85
CsiI ACCWGGT 1 cut(s) 475
Csp6I GTAC 1 cut(s) 338
CviAII CATG 3 cut(s) 187, 311, 505
CviJI RGCY 8 cut(s) 5, 28, 37, 93, 212, 232, 432, 461
CviKI_1 RGCY 8 cut(s) 5, 28, 37, 93, 212, 232, 432, 461
CviQI GTAC 1 cut(s) 338
DpnI GATC 4 cut(s) 216, 257, 472, 511
DpnII GATC 4 cut(s) 214, 255, 470, 509
DraIII CACNNNGTG 1 cut(s) 574
EaeI YGGCCR 3 cut(s) 3, 230, 459
Eam1104I CTCTTC 1 cut(s) 3
EarI CTCTTC 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 88
Eco24I GRGCYC 1 cut(s) 39
Eco31I GGTCTC 1 cut(s) 153
Eco47I GGWCC 1 cut(s) 85
Eco53kI GAGCTC 1 cut(s) 37
EcoICRI GAGCTC 1 cut(s) 37
EcoRI GAATTC 1 cut(s) 236
EcoRII CCWGG 2 cut(s) 367, 475
EcoT14I CCWWGG 1 cut(s) 88
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 88
Esp3I CGTCTC 1 cut(s) 456
FaeI CATG 3 cut(s) 190, 314, 508
FaiI YATR 7 cut(s) 54, 188, 312, 442, 483, 506, 580
FatI CATG 3 cut(s) 186, 310, 504
FbaI TGATCA 1 cut(s) 255
Fnu4HI GCNGC 1 cut(s) 462
FokI GGATG 3 cut(s) 147, 338, 387
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 1 cut(s) 462
FspBI CTAG 1 cut(s) 429
GluI GCNGC 1 cut(s) 462
HaeIII GGCC 3 cut(s) 5, 232, 461
HapII CCGG 1 cut(s) 229
Hin1II CATG 3 cut(s) 190, 314, 508
HincII GTYRAC 1 cut(s) 276
HindII GTYRAC 1 cut(s) 276
HinfI GANTC 2 cut(s) 363, 560
HpaII CCGG 1 cut(s) 229
HphI GGTGA 2 cut(s) 465, 560
Hpy166II GTNNAC 2 cut(s) 276, 521
Hpy188I TCNGA 1 cut(s) 362
Hpy8I GTNNAC 2 cut(s) 276, 521
HpyAV CCTTC 3 cut(s) 193, 294, 358
HpyCH4III ACNGT 1 cut(s) 376
HpyCH4IV ACGT 2 cut(s) 64, 491
HpyCH4V TGCA 5 cut(s) 56, 190, 485, 498, 527
HpyF10VI GCNNNNNNNGC 2 cut(s) 34, 320
HpySE526I ACGT 2 cut(s) 64, 491
Hsp92II CATG 3 cut(s) 190, 314, 508
KroI GCCGGC 1 cut(s) 228
KroNI GCCGGC 1 cut(s) 230
Ksp22I TGATCA 1 cut(s) 255
Kzo9I GATC 4 cut(s) 214, 255, 470, 509
LweI GCATC 1 cut(s) 3
MabI ACCWGGT 1 cut(s) 475
MaeI CTAG 1 cut(s) 429
MaeII ACGT 2 cut(s) 64, 491
MaeIII GTNAC 2 cut(s) 125, 552
MalI GATC 4 cut(s) 216, 257, 472, 511
MbiI CCGCTC 1 cut(s) 308
MboI GATC 4 cut(s) 214, 255, 470, 509
MboII GAAGA 2 cut(s) 20, 504
MflI RGATCY 1 cut(s) 509
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 2 cut(s) 40, 236
MlyI GAGTC 1 cut(s) 554
MnlI CCTC 2 cut(s) 118, 394
MroNI GCCGGC 1 cut(s) 228
MslI CAYNNNNRTG 1 cut(s) 503
MspI CCGG 1 cut(s) 229
MspR9I CCNGG 2 cut(s) 369, 477
MvaI CCWGG 2 cut(s) 369, 477
MvnI CGCG 1 cut(s) 573
MwoI GCNNNNNNNGC 2 cut(s) 34, 320
NaeI GCCGGC 1 cut(s) 230
NdeII GATC 4 cut(s) 214, 255, 470, 509
NgoMIV GCCGGC 1 cut(s) 228
NheI GCTAGC 1 cut(s) 428
NlaIII CATG 3 cut(s) 190, 314, 508
NlaIV GGNNCC 1 cut(s) 227
PdiI GCCGGC 1 cut(s) 230
PfeI GAWTC 1 cut(s) 363
PkrI GCNGC 1 cut(s) 463
PleI GAGTC 1 cut(s) 554
PpsI GAGTC 1 cut(s) 554
PshAI GACNNNNGTC 1 cut(s) 271
Psp124BI GAGCTC 1 cut(s) 39
Psp6I CCWGG 2 cut(s) 367, 475
PspGI CCWGG 2 cut(s) 367, 475
PspN4I GGNNCC 1 cut(s) 227
PspPI GGNCC 1 cut(s) 85
PsuI RGATCY 1 cut(s) 509
RsaI GTAC 1 cut(s) 339
RsaNI GTAC 1 cut(s) 338
RseI CAYNNNNRTG 1 cut(s) 503
SacI GAGCTC 1 cut(s) 39
SatI GCNGC 1 cut(s) 462
Sau3AI GATC 4 cut(s) 214, 255, 470, 509
Sau96I GGNCC 1 cut(s) 85
SchI GAGTC 1 cut(s) 554
ScrFI CCNGG 2 cut(s) 369, 477
SduI GDGCHC 1 cut(s) 39
SetI ASST 7 cut(s) 39, 67, 149, 185, 434, 478, 494
SexAI ACCWGGT 1 cut(s) 475
SfaNI GCATC 1 cut(s) 3
SinI GGWCC 1 cut(s) 85
SmiMI CAYNNNNRTG 1 cut(s) 503
Sse9I AATT 2 cut(s) 40, 236
SsiI CCGC 3 cut(s) 306, 462, 571
SspMI CTAG 1 cut(s) 429
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 367, 475
StyI CCWWGG 1 cut(s) 88
TaaI ACNGT 1 cut(s) 376
TaiI ACGT 2 cut(s) 67, 494
TasI AATT 2 cut(s) 40, 236
TauI GCSGC 1 cut(s) 464
TfiI GAWTC 1 cut(s) 363
TscAI CASTG 1 cut(s) 543
TspDTI ATGAA 5 cut(s) 175, 316, 340, 412, 429
TspGWI ACGGA 1 cut(s) 279
TspRI CASTG 1 cut(s) 543
VpaK11BI GGWCC 1 cut(s) 85
XapI RAATTY 1 cut(s) 236
XspI CTAG 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.