Rh1DG271000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000574
Physical Location & Seq
Reverse (-)
48718822 .. 48719481
660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG271000.1

Sequence Viewer

Length: 660 bp
ATGGCCACTGATAATGATCATCATATTCACTTCCCATGGCAATCAGCAGTCTTAAATAATCAACATGGTCACATGGTTGTCTACCTCAACATTGTAGTTCCAACTGTATCTGCTCCTTCTATTACTACTGATTCACCCTCTAACAATCTAGCTTTCATCATTCCGGTTCTCTTAACCTTCGTTCAGATCAAGTTCCCAGGGACGAACATGTTGTCTTCGCTGTTCGAAACACACCATACAACCACCATGGCCGCCATTGCAAGCTTGCTTGTCTATTGGCTGGCGCTAGAGGCCATGCAGAGATTCCCCGCTTACACTGTCAACTTTCGAGTTGTTAGGATGTTTTCAGGTTTGATCTCAATTGCTTCACTGCTGGCGCTTCTGCTTCCCGACTCTTGGGAGCATGTCCCATATTACATTTTGTTCGTGTCATATTGGGTGAGCCGAGGGTGTGGATTTCTACGAATGATGTATGGATTGGTTTACCGACGAGTTGTGGAGGAACTTCTTCAAAAGTTATGGTCATTCATCCACTACCGTCAACGAGTCCGAAGAACTGGTGGGCTATTGCCGTTTACTGTTATGGATACACATCATCGCACCTCGGTCCGTGGGCACTTGCCATTGACGGTCATGGATATGCATGATCGCATATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

219

Amino Acids

25.24

Weight (kDa)

9.41

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 81
AciI CCGC 2 cut(s) 252, 309
AcoI YGGCCR 2 cut(s) 3, 249
AfiI CCNNNNNNNGG 1 cut(s) 396
AflIII ACRYGT 1 cut(s) 207
AgsI TTSAA 1 cut(s) 512
AjnI CCWGG 1 cut(s) 196
AluBI AGCT 2 cut(s) 152, 264
AluI AGCT 2 cut(s) 152, 264
AoxI GGCC 3 cut(s) 3, 249, 291
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
Asp700I GAANNNNTTC 1 cut(s) 507
AspLEI GCGC 2 cut(s) 286, 379
AspS9I GGNCC 1 cut(s) 607
AsuHPI GGTGA 2 cut(s) 126, 451
AsuII TTCGAA 1 cut(s) 225
AvaII GGWCC 1 cut(s) 607
BaeGI GKGCMC 1 cut(s) 618
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 207
BceAI ACGGC 1 cut(s) 556
BciT130I CCWGG 1 cut(s) 198
BciVI GTATCC 1 cut(s) 580
BclI TGATCA 1 cut(s) 16
BfaI CTAG 2 cut(s) 149, 287
BfoI RGCGCY 2 cut(s) 287, 380
BfuI GTATCC 1 cut(s) 580
BisI GCNGC 1 cut(s) 252
BlsI GCNGC 1 cut(s) 253
Bme1390I CCNGG 1 cut(s) 198
Bme18I GGWCC 1 cut(s) 607
BmgT120I GGNCC 1 cut(s) 607
BmrFI CCNGG 1 cut(s) 198
BpiI GAAGAC 1 cut(s) 207
Bpu14I TTCGAA 1 cut(s) 225
BsaBI GATNNNNATC 2 cut(s) 15, 591
BsaJI CCNNGG 7 cut(s) 35, 196, 197, 246, 445, 603, 610
BsaWI WCCGGW 1 cut(s) 163
Bsc4I CCNNNNNNNGG 1 cut(s) 396
Bse1I ACTGG 1 cut(s) 562
Bse3DI GCAATG 1 cut(s) 255
Bse8I GATNNNNATC 2 cut(s) 15, 591
BseBI CCWGG 1 cut(s) 198
BseDI CCNNGG 7 cut(s) 35, 196, 197, 246, 445, 603, 610
BseGI GGATG 2 cut(s) 345, 528
BseJI GATNNNNATC 2 cut(s) 15, 591
BseLI CCNNNNNNNGG 1 cut(s) 396
BseMI GCAATG 1 cut(s) 255
BseNI ACTGG 1 cut(s) 562
BseSI GKGCMC 1 cut(s) 618
BshFI GGCC 3 cut(s) 5, 251, 293
BsiSI CCGG 1 cut(s) 164
BslFI GGGAC 2 cut(s) 214, 392
BslI CCNNNNNNNGG 1 cut(s) 396
BsmFI GGGAC 2 cut(s) 214, 392
BsnI GGCC 3 cut(s) 5, 251, 293
Bsp119I TTCGAA 1 cut(s) 225
Bsp1286I GDGCHC 1 cut(s) 618
Bsp143I GATC 4 cut(s) 16, 186, 354, 646
Bsp19I CCATGG 2 cut(s) 35, 246
BspACI CCGC 2 cut(s) 252, 309
BspANI GGCC 3 cut(s) 5, 251, 293
BspT104I TTCGAA 1 cut(s) 225
BsrDI GCAATG 1 cut(s) 255
BsrI ACTGG 1 cut(s) 562
BssECI CCNNGG 7 cut(s) 35, 196, 197, 246, 445, 603, 610
BssMI GATC 4 cut(s) 16, 186, 354, 646
BssT1I CCWWGG 2 cut(s) 35, 246
Bst2UI CCWGG 1 cut(s) 198
Bst4CI ACNGT 5 cut(s) 106, 319, 539, 580, 631
BstBI TTCGAA 1 cut(s) 225
BstC8I GCNNGC 4 cut(s) 262, 266, 282, 375
BstDSI CCRYGG 3 cut(s) 35, 246, 610
BstF5I GGATG 2 cut(s) 345, 528
BstH2I RGCGCY 2 cut(s) 287, 380
BstHHI GCGC 2 cut(s) 286, 379
BstKTI GATC 4 cut(s) 19, 189, 357, 649
BstMBI GATC 4 cut(s) 16, 186, 354, 646
BstMWI GCNNNNNNNGC 2 cut(s) 257, 290
BstNI CCWGG 1 cut(s) 198
BstNSI RCATGY 2 cut(s) 211, 407
BstSCI CCNGG 1 cut(s) 196
BstSLI GKGCMC 1 cut(s) 618
BstV2I GAAGAC 1 cut(s) 207
BsuI GTATCC 1 cut(s) 580
BsuRI GGCC 3 cut(s) 5, 251, 293
BtgI CCRYGG 3 cut(s) 35, 246, 610
BtgZI GCGATG 1 cut(s) 581
BtsCI GGATG 2 cut(s) 345, 528
BtsI GCAGTG 1 cut(s) 368
BtsIMutI CAGTG 3 cut(s) 6, 315, 368
Cac8I GCNNGC 4 cut(s) 262, 266, 282, 375
CfoI GCGC 2 cut(s) 286, 379
Cfr13I GGNCC 1 cut(s) 607
CpoI CGGWCCG 1 cut(s) 607
CspI CGGWCCG 1 cut(s) 607
CviAII CATG 9 cut(s) 36, 65, 73, 208, 247, 295, 404, 634, 644
CviJI RGCY 8 cut(s) 5, 152, 251, 264, 280, 293, 444, 565
CviKI_1 RGCY 8 cut(s) 5, 152, 251, 264, 280, 293, 444, 565
DpnI GATC 4 cut(s) 18, 188, 356, 648
DpnII GATC 4 cut(s) 16, 186, 354, 646
EaeI YGGCCR 2 cut(s) 3, 249
Eco130I CCWWGG 2 cut(s) 35, 246
Eco47I GGWCC 1 cut(s) 607
EcoRII CCWGG 1 cut(s) 196
EcoT14I CCWWGG 2 cut(s) 35, 246
EcoT22I ATGCAT 1 cut(s) 645
ErhI CCWWGG 2 cut(s) 35, 246
FaeI CATG 9 cut(s) 39, 68, 76, 211, 250, 298, 407, 637, 647
FaqI GGGAC 2 cut(s) 214, 392
FatI CATG 9 cut(s) 35, 64, 72, 207, 246, 294, 403, 633, 643
FauI CCCGC 1 cut(s) 316
FbaI TGATCA 1 cut(s) 16
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 1 cut(s) 252
FokI GGATG 2 cut(s) 352, 515
Fsp4HI GCNGC 1 cut(s) 252
FspBI CTAG 2 cut(s) 149, 287
GlaI GCGC 2 cut(s) 285, 378
GluI GCNGC 1 cut(s) 252
HaeII RGCGCY 2 cut(s) 287, 380
HaeIII GGCC 3 cut(s) 5, 251, 293
HapII CCGG 1 cut(s) 164
HhaI GCGC 2 cut(s) 286, 379
Hin1II CATG 9 cut(s) 39, 68, 76, 211, 250, 298, 407, 637, 647
Hin6I GCGC 2 cut(s) 284, 377
HinP1I GCGC 2 cut(s) 284, 377
HincII GTYRAC 2 cut(s) 322, 542
HindII GTYRAC 2 cut(s) 322, 542
HindIII AAGCTT 1 cut(s) 262
HinfI GANTC 4 cut(s) 131, 303, 392, 546
HpaII CCGG 1 cut(s) 164
HphI GGTGA 2 cut(s) 126, 451
Hpy166II GTNNAC 5 cut(s) 82, 322, 484, 542, 576
Hpy188I TCNGA 2 cut(s) 186, 551
Hpy188III TCNNGA 1 cut(s) 389
Hpy8I GTNNAC 5 cut(s) 82, 322, 484, 542, 576
Hpy99I CGWCG 1 cut(s) 492
HpyAV CCTTC 2 cut(s) 126, 187
HpyCH4III ACNGT 5 cut(s) 106, 319, 539, 580, 631
HpyCH4V TGCA 3 cut(s) 260, 298, 643
HpyF10VI GCNNNNNNNGC 2 cut(s) 257, 290
Hsp92II CATG 9 cut(s) 39, 68, 76, 211, 250, 298, 407, 637, 647
HspAI GCGC 2 cut(s) 284, 377
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 4 cut(s) 16, 186, 354, 646
LmnI GCTCC 2 cut(s) 118, 400
LpnPI CCDG 7 cut(s) 177, 183, 210, 266, 333, 359, 543
MaeI CTAG 2 cut(s) 149, 287
MaeIII GTNAC 1 cut(s) 68
MalI GATC 4 cut(s) 18, 188, 356, 648
MboI GATC 4 cut(s) 16, 186, 354, 646
MboII GAAGA 3 cut(s) 207, 500, 564
MfeI CAATTG 1 cut(s) 360
MhlI GDGCHC 1 cut(s) 618
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 360
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 386, 555
MmeI TCCRAC 1 cut(s) 125
MnlI CCTC 6 cut(s) 95, 148, 283, 440, 493, 613
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 645
MroXI GAANNNNTTC 1 cut(s) 507
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 53, 173
MslI CAYNNNNRTG 1 cut(s) 638
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 164
MspR9I CCNGG 1 cut(s) 198
MunI CAATTG 1 cut(s) 360
MvaI CCWGG 1 cut(s) 198
MwoI GCNNNNNNNGC 2 cut(s) 257, 290
NcoI CCATGG 2 cut(s) 35, 246
NdeII GATC 4 cut(s) 16, 186, 354, 646
NlaIII CATG 9 cut(s) 39, 68, 76, 211, 250, 298, 407, 637, 647
NmeAIII GCCGAG 1 cut(s) 470
NmuCI GTSAC 1 cut(s) 68
NsiI ATGCAT 1 cut(s) 645
NspI RCATGY 2 cut(s) 211, 407
NspV TTCGAA 1 cut(s) 225
PasI CCCWGGG 1 cut(s) 197
PciI ACATGT 1 cut(s) 207
PdmI GAANNNNTTC 1 cut(s) 507
PfeI GAWTC 2 cut(s) 131, 303
PkrI GCNGC 1 cut(s) 253
PleI GAGTC 2 cut(s) 386, 554
PpsI GAGTC 2 cut(s) 386, 554
PscI ACATGT 1 cut(s) 207
Psp6I CCWGG 1 cut(s) 196
PspGI CCWGG 1 cut(s) 196
PspPI GGNCC 1 cut(s) 607
RseI CAYNNNNRTG 1 cut(s) 638
Rsr2I CGGWCCG 1 cut(s) 607
RsrII CGGWCCG 1 cut(s) 607
SaqAI TTAA 2 cut(s) 53, 173
SatI GCNGC 1 cut(s) 252
Sau3AI GATC 4 cut(s) 16, 186, 354, 646
Sau96I GGNCC 1 cut(s) 607
SchI GAGTC 2 cut(s) 386, 555
ScrFI CCNGG 1 cut(s) 198
SduI GDGCHC 1 cut(s) 618
SetI ASST 6 cut(s) 87, 154, 179, 266, 352, 605
SfuI TTCGAA 1 cut(s) 225
SinI GGWCC 1 cut(s) 607
SmiMI CAYNNNNRTG 1 cut(s) 638
Sse9I AATT 1 cut(s) 360
SsiI CCGC 2 cut(s) 252, 309
SspMI CTAG 2 cut(s) 149, 287
StyD4I CCNGG 1 cut(s) 196
StyI CCWWGG 2 cut(s) 35, 246
TaaI ACNGT 5 cut(s) 106, 319, 539, 580, 631
TaqI TCGA 2 cut(s) 225, 328
TaqII GACCGA 1 cut(s) 595
TasI AATT 1 cut(s) 360
TauI GCSGC 1 cut(s) 254
TfiI GAWTC 2 cut(s) 131, 303
Tru1I TTAA 2 cut(s) 53, 173
Tru9I TTAA 2 cut(s) 53, 173
TscAI CASTG 3 cut(s) 13, 322, 375
TseFI GTSAC 1 cut(s) 68
Tsp45I GTSAC 1 cut(s) 68
TspDTI ATGAA 2 cut(s) 145, 517
TspGWI ACGGA 1 cut(s) 599
TspRI CASTG 3 cut(s) 13, 322, 375
VpaK11BI GGWCC 1 cut(s) 607
XceI RCATGY 2 cut(s) 211, 407
XmiI GTMKAC 1 cut(s) 81
XmnI GAANNNNTTC 1 cut(s) 507
XspI CTAG 2 cut(s) 149, 287
Zsp2I ATGCAT 1 cut(s) 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.