Rroxscaffold_1G00012170

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000574
Physical Location & Seq
Reverse (-)
15027014 .. 15028145
1132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00012170.1

Sequence Viewer

Length: 555 bp
ATGATGAAGGACAAACTTCTTCGTGCAACACAGCCCTCATTCAACATTCTGGTCATCATTAATCAACAAGGGCTTGCTGCTGCTGCTCCACCTCCTCCTCCTCCTTCCACTTCAACTTCCACTCATGCCATTTTCATTTTCATCGTCAGTGTGTTCGTCTCCTTTGTCCAAATCAAGTTCCCAGAAATTCAGACCTCTTCACCATTTGAAACGCATCACACAACCATCATGGTTTTCTTTGCTAGCATAATTTCATATTGCTCATCTCTTTGGGCCACGAAAAGATTTCCTAGTTACGCCTATCTTTTTTATGTTATCATAAAATTTGCTGGTCTACTTGCACTAGCTTCATTGCTTTCACTTCTTCTGCCGAGCTCCTGTCACCGTGTGCCATACATGATGCTAATCTTCTATTTCATTGTGGAGTATTTACAGTTGCTCCGAATGCCATGTCAATTAACTTACCGGCAAGCCACTCATGGATCTGTGCCAGGCATTCATCGGAGGTGTGAAGTATTGCCGCTGACATACATGGATTTGCCTCCGATAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

184

Amino Acids

20.82

Weight (kDa)

9.3

Isoelectric Point (pI)

62.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 334
AciI CCGC 1 cut(s) 521
AclWI GGATC 1 cut(s) 490
AcsI RAATTY 2 cut(s) 186, 323
AdeI CACNNNGTG 1 cut(s) 388
AgsI TTSAA 3 cut(s) 43, 114, 209
AjnI CCWGG 1 cut(s) 490
AluBI AGCT 2 cut(s) 347, 375
AluI AGCT 2 cut(s) 347, 375
Alw21I GWGCWC 1 cut(s) 377
Alw26I GTCTC 1 cut(s) 163
AlwI GGATC 1 cut(s) 490
AoxI GGCC 1 cut(s) 273
ApeKI GCWGC 3 cut(s) 77, 80, 83
ApoI RAATTY 2 cut(s) 186, 323
ArsI GACNNNNNNTTYG 2 cut(s) 436, 468
AseI ATTAAT 1 cut(s) 60
AspS9I GGNCC 1 cut(s) 273
AsuHPI GGTGA 2 cut(s) 192, 374
AsuNHI GCTAGC 1 cut(s) 242
BanII GRGCYC 1 cut(s) 377
Bbv12I GWGCWC 1 cut(s) 377
BbvI GCAGC 3 cut(s) 64, 67, 70
BccI CCATC 1 cut(s) 233
BciT130I CCWGG 1 cut(s) 492
BcoDI GTCTC 1 cut(s) 163
BfaI CTAG 3 cut(s) 243, 291, 344
BisI GCNGC 4 cut(s) 78, 81, 84, 521
BlsI GCNGC 4 cut(s) 79, 82, 85, 522
Bme1390I CCNGG 1 cut(s) 492
BmgT120I GGNCC 1 cut(s) 273
BmrFI CCNGG 1 cut(s) 492
BmsI GCATC 2 cut(s) 223, 390
BmtI GCTAGC 1 cut(s) 246
BsaBI GATNNNNATC 1 cut(s) 404
BsaXI ACNNNNNCTCC 2 cut(s) 423, 453
Bse118I RCCGGY 1 cut(s) 465
Bse3DI GCAATG 1 cut(s) 350
Bse8I GATNNNNATC 1 cut(s) 404
BseBI CCWGG 1 cut(s) 492
BseJI GATNNNNATC 1 cut(s) 404
BseMI GCAATG 1 cut(s) 350
BseRI GAGGAG 3 cut(s) 84, 87, 90
BseXI GCAGC 3 cut(s) 64, 67, 70
BshFI GGCC 1 cut(s) 275
BsiHKAI GWGCWC 1 cut(s) 377
BsiSI CCGG 1 cut(s) 466
BsmAI GTCTC 1 cut(s) 163
BsmBI CGTCTC 1 cut(s) 163
BsmI GAATGC 2 cut(s) 450, 495
BsnI GGCC 1 cut(s) 275
Bsp1286I GDGCHC 1 cut(s) 377
Bsp143I GATC 1 cut(s) 482
BspACI CCGC 1 cut(s) 521
BspANI GGCC 1 cut(s) 275
BspOI GCTAGC 1 cut(s) 246
BspPI GGATC 1 cut(s) 490
BsrDI GCAATG 1 cut(s) 350
BsrFI RCCGGY 1 cut(s) 465
BssAI RCCGGY 1 cut(s) 465
BssMI GATC 1 cut(s) 482
Bst2UI CCWGG 1 cut(s) 492
Bst4CI ACNGT 2 cut(s) 386, 435
Bst6I CTCTTC 1 cut(s) 202
BstC8I GCNNGC 3 cut(s) 75, 244, 471
BstKTI GATC 1 cut(s) 485
BstMAI GTCTC 1 cut(s) 163
BstMBI GATC 1 cut(s) 482
BstMWI GCNNNNNNNGC 2 cut(s) 83, 445
BstNI CCWGG 1 cut(s) 492
BstSCI CCNGG 1 cut(s) 490
BstV1I GCAGC 3 cut(s) 64, 67, 70
BstX2I RGATCY 1 cut(s) 482
BstYI RGATCY 1 cut(s) 482
BsuRI GGCC 1 cut(s) 275
BtsIMutI CAGTG 1 cut(s) 154
Cac8I GCNNGC 3 cut(s) 75, 244, 471
Cfr10I RCCGGY 1 cut(s) 465
Cfr13I GGNCC 1 cut(s) 273
CviAII CATG 6 cut(s) 125, 229, 397, 450, 479, 532
CviJI RGCY 6 cut(s) 34, 73, 275, 347, 375, 473
CviKI_1 RGCY 6 cut(s) 34, 73, 275, 347, 375, 473
DpnI GATC 1 cut(s) 484
DpnII GATC 1 cut(s) 482
DraIII CACNNNGTG 1 cut(s) 388
Eam1104I CTCTTC 1 cut(s) 202
EarI CTCTTC 1 cut(s) 202
Ecl136II GAGCTC 1 cut(s) 375
Eco24I GRGCYC 1 cut(s) 377
Eco53kI GAGCTC 1 cut(s) 375
EcoICRI GAGCTC 1 cut(s) 375
EcoRII CCWGG 1 cut(s) 490
EcoT38I GRGCYC 1 cut(s) 377
Esp3I CGTCTC 1 cut(s) 163
FaeI CATG 6 cut(s) 128, 232, 400, 453, 482, 535
FatI CATG 6 cut(s) 124, 228, 396, 449, 478, 531
FblI GTMKAC 1 cut(s) 334
Fnu4HI GCNGC 4 cut(s) 78, 81, 84, 521
FriOI GRGCYC 1 cut(s) 377
Fsp4HI GCNGC 4 cut(s) 78, 81, 84, 521
FspBI CTAG 3 cut(s) 243, 291, 344
GluI GCNGC 4 cut(s) 78, 81, 84, 521
HaeIII GGCC 1 cut(s) 275
HapII CCGG 1 cut(s) 466
Hin1II CATG 6 cut(s) 128, 232, 400, 453, 482, 535
HpaII CCGG 1 cut(s) 466
HphI GGTGA 2 cut(s) 192, 374
Hpy166II GTNNAC 1 cut(s) 335
Hpy188I TCNGA 4 cut(s) 192, 443, 504, 546
Hpy8I GTNNAC 1 cut(s) 335
HpyAV CCTTC 1 cut(s) 114
HpyCH4III ACNGT 2 cut(s) 386, 435
HpyCH4V TGCA 2 cut(s) 26, 341
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 445
Hsp92II CATG 6 cut(s) 128, 232, 400, 453, 482, 535
Kzo9I GATC 1 cut(s) 482
LmnI GCTCC 3 cut(s) 91, 380, 444
LpnPI CCDG 7 cut(s) 35, 195, 315, 391, 477, 479, 504
Lsp1109I GCAGC 3 cut(s) 64, 67, 70
LweI GCATC 2 cut(s) 223, 390
MaeI CTAG 3 cut(s) 243, 291, 344
MaeIII GTNAC 2 cut(s) 293, 380
MalI GATC 1 cut(s) 484
MboI GATC 1 cut(s) 482
MboII GAAGA 4 cut(s) 11, 189, 356, 400
MflI RGATCY 1 cut(s) 482
MhlI GDGCHC 1 cut(s) 377
MluCI AATT 5 cut(s) 186, 249, 323, 455, 550
MnlI CCTC 8 cut(s) 46, 102, 105, 108, 111, 205, 498, 552
MseI TTAA 2 cut(s) 60, 458
MspA1I CMGCKG 1 cut(s) 523
MspI CCGG 1 cut(s) 466
MspR9I CCNGG 1 cut(s) 492
Mva1269I GAATGC 2 cut(s) 450, 495
MvaI CCWGG 1 cut(s) 492
MwoI GCNNNNNNNGC 2 cut(s) 83, 445
NdeII GATC 1 cut(s) 482
NheI GCTAGC 1 cut(s) 242
NlaIII CATG 6 cut(s) 128, 232, 400, 453, 482, 535
NmeAIII GCCGAG 1 cut(s) 396
NmuCI GTSAC 1 cut(s) 380
PctI GAATGC 2 cut(s) 450, 495
PkrI GCNGC 4 cut(s) 79, 82, 85, 522
PshBI ATTAAT 1 cut(s) 60
Psp124BI GAGCTC 1 cut(s) 377
Psp6I CCWGG 1 cut(s) 490
PspGI CCWGG 1 cut(s) 490
PspPI GGNCC 1 cut(s) 273
PsuI RGATCY 1 cut(s) 482
SacI GAGCTC 1 cut(s) 377
SaqAI TTAA 2 cut(s) 60, 458
SatI GCNGC 4 cut(s) 78, 81, 84, 521
Sau3AI GATC 1 cut(s) 482
Sau96I GGNCC 1 cut(s) 273
ScrFI CCNGG 1 cut(s) 492
SduI GDGCHC 1 cut(s) 377
SetI ASST 5 cut(s) 94, 197, 349, 377, 509
SfaNI GCATC 2 cut(s) 223, 390
Sse9I AATT 5 cut(s) 186, 249, 323, 455, 550
SsiI CCGC 1 cut(s) 521
SspMI CTAG 3 cut(s) 243, 291, 344
SstI GAGCTC 1 cut(s) 377
StyD4I CCNGG 1 cut(s) 490
TaaI ACNGT 2 cut(s) 386, 435
TasI AATT 5 cut(s) 186, 249, 323, 455, 550
TauI GCSGC 1 cut(s) 523
Tru1I TTAA 2 cut(s) 60, 458
Tru9I TTAA 2 cut(s) 60, 458
TscAI CASTG 1 cut(s) 154
TseFI GTSAC 1 cut(s) 380
TseI GCWGC 3 cut(s) 77, 80, 83
Tsp45I GTSAC 1 cut(s) 380
TspDTI ATGAA 7 cut(s) 20, 124, 130, 243, 339, 406, 488
TspRI CASTG 1 cut(s) 154
VspI ATTAAT 1 cut(s) 60
XapI RAATTY 2 cut(s) 186, 323
XmiI GTMKAC 1 cut(s) 334
XspI CTAG 3 cut(s) 243, 291, 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.