Rroxscaffold_4G00298110

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000574
Physical Location & Seq
Forward (+)
17964972 .. 17969485
4514 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00298110.1

Sequence Viewer

Length: 849 bp
ATGGCCACTGATAATGATCATCATATTCGCCTCCAATGGCAATCAGCAGTCTTAAATAATCAACATGGTCACATGGTTGTCTACCTCAACATTGTAGTTCCAACTGTATCTAGATCTGCTTCTTCATCTTCTTCTTCTACTACTATTGATTCACCCTCAAACAGTCTATTTTTCATCATTCCGGTTCTCTTAACCTTCGTTCAGATCAAGTTCTCAGGGATGAACATGTGGTCTGCGCTGTTCGAAACACACCATACAACCACCATGGCCGCCATTGTAAGCTTGCTTGTCTATTGGCTGGTGCTAGAGGCCATGCAGAGATTCCCCGCTTACACGGTCAACTTTCGAGTTGTTAGGATGTTTTCAGGTTTGATCTCAATTGCTTCACTGCTGGCGCTTCTGCTTCCCGACTCTTGGGAGCATGTCCCATATTACATTTTATTCGTGTCATATTGGGTGAGCCGAGGTTTTGGATTTCTACGAATGATGTATGGATTGGTTTACCGACGAGTTGTGGAGGAACTTCTTCAAAAGTTACGGTCATTCATCCACTACCGTCAACTAGTCCGAAGAACTGGTGGGCTCTTGCCGTTTACACATTTATTATCTCGGAGAGGAGGCCACCGAGCAATATGGAGCGACCTTGATAGGCGAAACCTAGGGTTTCGTTCTTTGGCGGCTGCGATCTTACGGTCCCCAGACCAGGCAGTGATGGCGAGGAGAGGCGGTGCAGTCTTGGTCGTCGGCGTCGGTGTTCCAGATCGTTTGCTCGACGGAAATGATGAGGTGCTCCGACTGTTCGGGGAATCATCTATTAAACCTTGTCCACCGGATAGCACATCCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

282

Amino Acids

31.84

Weight (kDa)

10.11

Isoelectric Point (pI)

54.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 81
AciI CCGC 5 cut(s) 270, 327, 677, 726, 843
AcoI YGGCCR 2 cut(s) 3, 267
AcyI GRCGYC 1 cut(s) 747
AfiI CCNNNNNNNGG 2 cut(s) 414, 703
AflIII ACRYGT 1 cut(s) 225
AgsI TTSAA 1 cut(s) 530
AhlI ACTAGT 1 cut(s) 562
AjnI CCWGG 1 cut(s) 702
AluBI AGCT 1 cut(s) 282
AluI AGCT 1 cut(s) 282
Alw21I GWGCWC 1 cut(s) 792
AoxI GGCC 4 cut(s) 3, 267, 309, 619
ApeKI GCWGC 1 cut(s) 680
Asp700I GAANNNNTTC 1 cut(s) 525
AspA2I CCTAGG 1 cut(s) 658
AspLEI GCGC 2 cut(s) 238, 397
AspS9I GGNCC 1 cut(s) 693
AsuHPI GGTGA 2 cut(s) 144, 469
AsuII TTCGAA 1 cut(s) 243
AvaII GGWCC 1 cut(s) 693
AvrII CCTAGG 1 cut(s) 658
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 585
Bbv12I GWGCWC 1 cut(s) 792
BbvI GCAGC 1 cut(s) 667
BccI CCATC 1 cut(s) 706
BceAI ACGGC 1 cut(s) 574
BciT130I CCWGG 1 cut(s) 704
BclI TGATCA 1 cut(s) 16
BcuI ACTAGT 1 cut(s) 562
BfaI CTAG 4 cut(s) 111, 305, 563, 659
BfoI RGCGCY 1 cut(s) 398
BglII AGATCT 1 cut(s) 113
BisI GCNGC 3 cut(s) 270, 678, 681
BlnI CCTAGG 1 cut(s) 658
BlsI GCNGC 3 cut(s) 271, 679, 682
Bme1390I CCNGG 1 cut(s) 704
Bme18I GGWCC 1 cut(s) 693
BmgT120I GGNCC 1 cut(s) 693
BmiI GGNNCC 1 cut(s) 695
BmrFI CCNGG 1 cut(s) 704
Bpu14I TTCGAA 1 cut(s) 243
BsaBI GATNNNNATC 1 cut(s) 15
BsaHI GRCGYC 1 cut(s) 747
BsaJI CCNNGG 3 cut(s) 264, 463, 658
BsaWI WCCGGW 2 cut(s) 181, 829
BsaXI ACNNNNNCTCC 2 cut(s) 712, 742
Bsc4I CCNNNNNNNGG 2 cut(s) 414, 703
Bse1I ACTGG 1 cut(s) 580
Bse8I GATNNNNATC 1 cut(s) 15
BseBI CCWGG 1 cut(s) 704
BseDI CCNNGG 3 cut(s) 264, 463, 658
BseGI GGATG 4 cut(s) 225, 363, 546, 839
BseJI GATNNNNATC 1 cut(s) 15
BseLI CCNNNNNNNGG 2 cut(s) 414, 703
BseMII CTCAG 1 cut(s) 228
BseNI ACTGG 1 cut(s) 580
BseRI GAGGAG 2 cut(s) 630, 733
BseXI GCAGC 1 cut(s) 667
BsgI GTGCAG 1 cut(s) 750
BshFI GGCC 4 cut(s) 5, 269, 311, 621
BsiHKAI GWGCWC 1 cut(s) 792
BsiSI CCGG 2 cut(s) 182, 830
BslFI GGGAC 2 cut(s) 410, 679
BslI CCNNNNNNNGG 2 cut(s) 414, 703
BsmFI GGGAC 2 cut(s) 410, 679
BsnI GGCC 4 cut(s) 5, 269, 311, 621
Bsp119I TTCGAA 1 cut(s) 243
Bsp1286I GDGCHC 2 cut(s) 585, 792
Bsp143I GATC 6 cut(s) 16, 113, 204, 372, 684, 760
Bsp19I CCATGG 1 cut(s) 264
BspACI CCGC 5 cut(s) 270, 327, 677, 726, 843
BspANI GGCC 4 cut(s) 5, 269, 311, 621
BspCNI CTCAG 1 cut(s) 227
BspLI GGNNCC 1 cut(s) 695
BspT104I TTCGAA 1 cut(s) 243
BsrI ACTGG 1 cut(s) 580
BssECI CCNNGG 3 cut(s) 264, 463, 658
BssMI GATC 6 cut(s) 16, 113, 204, 372, 684, 760
BssNI GRCGYC 1 cut(s) 747
BssT1I CCWWGG 2 cut(s) 264, 658
Bst2UI CCWGG 1 cut(s) 704
Bst4CI ACNGT 7 cut(s) 106, 164, 337, 540, 557, 693, 798
BstACI GRCGYC 1 cut(s) 747
BstBI TTCGAA 1 cut(s) 243
BstC8I GCNNGC 2 cut(s) 284, 393
BstDEI CTNAG 1 cut(s) 214
BstDSI CCRYGG 1 cut(s) 264
BstF5I GGATG 4 cut(s) 225, 363, 546, 839
BstH2I RGCGCY 1 cut(s) 398
BstHHI GCGC 2 cut(s) 238, 397
BstKTI GATC 6 cut(s) 19, 116, 207, 375, 687, 763
BstMBI GATC 6 cut(s) 16, 113, 204, 372, 684, 760
BstMWI GCNNNNNNNGC 1 cut(s) 713
BstNI CCWGG 1 cut(s) 704
BstNSI RCATGY 2 cut(s) 229, 425
BstSCI CCNGG 1 cut(s) 702
BstV1I GCAGC 1 cut(s) 667
BstX2I RGATCY 1 cut(s) 113
BstYI RGATCY 1 cut(s) 113
BsuRI GGCC 4 cut(s) 5, 269, 311, 621
BtgI CCRYGG 1 cut(s) 264
BtsCI GGATG 4 cut(s) 225, 363, 546, 839
BtsI GCAGTG 2 cut(s) 386, 714
BtsIMutI CAGTG 3 cut(s) 6, 386, 714
Cac8I GCNNGC 2 cut(s) 284, 393
CfoI GCGC 2 cut(s) 238, 397
Cfr13I GGNCC 1 cut(s) 693
CseI GACGC 1 cut(s) 736
CviAII CATG 6 cut(s) 65, 73, 226, 265, 313, 422
CviJI RGCY 9 cut(s) 5, 269, 282, 298, 311, 462, 583, 621, 680
CviKI_1 RGCY 9 cut(s) 5, 269, 282, 298, 311, 462, 583, 621, 680
DdeI CTNAG 1 cut(s) 214
DpnI GATC 6 cut(s) 18, 115, 206, 374, 686, 762
DpnII GATC 6 cut(s) 16, 113, 204, 372, 684, 760
EaeI YGGCCR 2 cut(s) 3, 267
Eco130I CCWWGG 2 cut(s) 264, 658
Eco24I GRGCYC 1 cut(s) 585
Eco47I GGWCC 1 cut(s) 693
EcoRII CCWGG 1 cut(s) 702
EcoT14I CCWWGG 2 cut(s) 264, 658
EcoT38I GRGCYC 1 cut(s) 585
ErhI CCWWGG 2 cut(s) 264, 658
FaeI CATG 6 cut(s) 68, 76, 229, 268, 316, 425
FaqI GGGAC 2 cut(s) 410, 679
FatI CATG 6 cut(s) 64, 72, 225, 264, 312, 421
FauI CCCGC 1 cut(s) 334
FbaI TGATCA 1 cut(s) 16
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 3 cut(s) 270, 678, 681
FokI GGATG 4 cut(s) 232, 370, 533, 826
FriOI GRGCYC 1 cut(s) 585
Fsp4HI GCNGC 3 cut(s) 270, 678, 681
FspBI CTAG 4 cut(s) 111, 305, 563, 659
GlaI GCGC 2 cut(s) 237, 396
GluI GCNGC 3 cut(s) 270, 678, 681
HaeII RGCGCY 1 cut(s) 398
HaeIII GGCC 4 cut(s) 5, 269, 311, 621
HapII CCGG 2 cut(s) 182, 830
HgaI GACGC 1 cut(s) 736
HhaI GCGC 2 cut(s) 238, 397
Hin1I GRCGYC 1 cut(s) 747
Hin1II CATG 6 cut(s) 68, 76, 229, 268, 316, 425
Hin6I GCGC 2 cut(s) 236, 395
HinP1I GCGC 2 cut(s) 236, 395
HincII GTYRAC 2 cut(s) 340, 560
HindII GTYRAC 2 cut(s) 340, 560
HindIII AAGCTT 1 cut(s) 280
HinfI GANTC 4 cut(s) 149, 321, 410, 806
HpaII CCGG 2 cut(s) 182, 830
HphI GGTGA 2 cut(s) 144, 469
Hpy166II GTNNAC 6 cut(s) 82, 340, 502, 560, 594, 827
Hpy188I TCNGA 4 cut(s) 204, 569, 612, 794
Hpy188III TCNNGA 3 cut(s) 111, 407, 758
Hpy8I GTNNAC 6 cut(s) 82, 340, 502, 560, 594, 827
Hpy99I CGWCG 4 cut(s) 510, 746, 752, 776
HpyAV CCTTC 1 cut(s) 205
HpyCH4III ACNGT 7 cut(s) 106, 164, 337, 540, 557, 693, 798
HpyCH4V TGCA 2 cut(s) 316, 731
HpyF10VI GCNNNNNNNGC 1 cut(s) 713
HpyF3I CTNAG 1 cut(s) 214
Hsp92I GRCGYC 1 cut(s) 747
Hsp92II CATG 6 cut(s) 68, 76, 229, 268, 316, 425
HspAI GCGC 2 cut(s) 236, 395
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 6 cut(s) 16, 113, 204, 372, 684, 760
LmnI GCTCC 3 cut(s) 418, 636, 795
Lsp1109I GCAGC 1 cut(s) 667
MaeI CTAG 4 cut(s) 111, 305, 563, 659
MaeIII GTNAC 2 cut(s) 68, 534
MalI GATC 6 cut(s) 18, 115, 206, 374, 686, 762
MboI GATC 6 cut(s) 16, 113, 204, 372, 684, 760
MboII GAAGA 6 cut(s) 114, 120, 123, 126, 518, 582
MfeI CAATTG 1 cut(s) 378
MflI RGATCY 1 cut(s) 113
MhlI GDGCHC 2 cut(s) 585, 792
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 378
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 2 cut(s) 125, 817
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 525
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 53, 191, 816, 847
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 182, 830
MspR9I CCNGG 1 cut(s) 704
MunI CAATTG 1 cut(s) 378
MvaI CCWGG 1 cut(s) 704
MwoI GCNNNNNNNGC 1 cut(s) 713
NcoI CCATGG 1 cut(s) 264
NdeII GATC 6 cut(s) 16, 113, 204, 372, 684, 760
NlaIII CATG 6 cut(s) 68, 76, 229, 268, 316, 425
NlaIV GGNNCC 1 cut(s) 695
NmeAIII GCCGAG 1 cut(s) 488
NmuCI GTSAC 1 cut(s) 68
NspI RCATGY 2 cut(s) 229, 425
NspV TTCGAA 1 cut(s) 243
PciI ACATGT 1 cut(s) 225
PdmI GAANNNNTTC 1 cut(s) 525
PfeI GAWTC 3 cut(s) 149, 321, 806
PkrI GCNGC 3 cut(s) 271, 679, 682
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
PscI ACATGT 1 cut(s) 225
Psp6I CCWGG 1 cut(s) 702
PspGI CCWGG 1 cut(s) 702
PspN4I GGNNCC 1 cut(s) 695
PspPI GGNCC 1 cut(s) 693
PsuI RGATCY 1 cut(s) 113
SaqAI TTAA 4 cut(s) 53, 191, 816, 847
SatI GCNGC 3 cut(s) 270, 678, 681
Sau3AI GATC 6 cut(s) 16, 113, 204, 372, 684, 760
Sau96I GGNCC 1 cut(s) 693
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 1 cut(s) 704
SduI GDGCHC 2 cut(s) 585, 792
SetI ASST 9 cut(s) 87, 197, 284, 370, 469, 645, 660, 789, 823
SfuI TTCGAA 1 cut(s) 243
SinI GGWCC 1 cut(s) 693
SpeI ACTAGT 1 cut(s) 562
Sse9I AATT 1 cut(s) 378
SsiI CCGC 5 cut(s) 270, 327, 677, 726, 843
SspMI CTAG 4 cut(s) 111, 305, 563, 659
StyD4I CCNGG 1 cut(s) 702
StyI CCWWGG 2 cut(s) 264, 658
TaaI ACNGT 7 cut(s) 106, 164, 337, 540, 557, 693, 798
TaqI TCGA 3 cut(s) 243, 346, 771
TasI AATT 1 cut(s) 378
TauI GCSGC 2 cut(s) 272, 680
TfiI GAWTC 3 cut(s) 149, 321, 806
Tru1I TTAA 4 cut(s) 53, 191, 816, 847
Tru9I TTAA 4 cut(s) 53, 191, 816, 847
TscAI CASTG 3 cut(s) 13, 393, 714
TseFI GTSAC 1 cut(s) 68
TseI GCWGC 1 cut(s) 680
Tsp45I GTSAC 1 cut(s) 68
TspDTI ATGAA 4 cut(s) 114, 163, 236, 535
TspGWI ACGGA 1 cut(s) 789
TspRI CASTG 3 cut(s) 13, 393, 714
VpaK11BI GGWCC 1 cut(s) 693
XbaI TCTAGA 1 cut(s) 110
XceI RCATGY 2 cut(s) 229, 425
XmaJI CCTAGG 1 cut(s) 658
XmiI GTMKAC 1 cut(s) 81
XmnI GAANNNNTTC 1 cut(s) 525
XspI CTAG 4 cut(s) 111, 305, 563, 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.