Prupe.2G180100_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000574
Physical Location & Seq
Forward (+)
22354591 .. 22356120
1530 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G180100.1

Sequence Viewer

Length: 513 bp
ATGCAATCACTGCCATGGGCCTCTAGAGAAAAAATTGGCAAGAGTATTACTAATATTAGTGGGGCATGCAATTTCCAGATGTCAGTACTTGTTGCCCTAATTCCAATCAAGTTCCCGGGAATATCAATGTTGTCTTCTCCATTTGAAACCCATTGTACCACCACCATGGTTGCCATTGCAAGCTTACTTGCTTACAGCTCCGCTGTTGCGGCTAGGCTAAGATTCCCTACTTACTCTCCCACCAACTTTCGTTTGGCCATCATATTTTCTGCTTTACTTTCAGTTGCATCTCTGCTATCACTTCTCTTTCCTGACTCCTGGCACCACATCCCATACGTGATCTTCGTCTTCTACTATTTCATGGCCGAGGTTTCTTCAGTCTTATATATTCCTTTTTCTTTCTTATTTCTTTGTAACTTGTTTTTCTTTCAAGTACTTACTCCTTACCATTTGAGTAAGACTTGTATTCTTATCAAACTTGATACAAGTGCTCATAACTTGAAGCTCTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

171

Amino Acids

19.07

Weight (kDa)

9.06

Isoelectric Point (pI)

39.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 321
AciI CCGC 2 cut(s) 201, 209
AcoI YGGCCR 2 cut(s) 255, 363
AcuI CTGAAG 1 cut(s) 360
AfaI GTAC 3 cut(s) 87, 157, 435
AgsI TTSAA 3 cut(s) 146, 431, 502
AjnI CCWGG 1 cut(s) 317
AluBI AGCT 3 cut(s) 183, 198, 505
AluI AGCT 3 cut(s) 183, 198, 505
Alw21I GWGCWC 1 cut(s) 493
Ama87I CYCGRG 1 cut(s) 115
AoxI GGCC 3 cut(s) 18, 255, 363
AspS9I GGNCC 1 cut(s) 18
AsuC2I CCSGG 2 cut(s) 116, 117
AvaI CYCGRG 1 cut(s) 115
BalI TGGCCA 1 cut(s) 257
BanI GGYRCC 1 cut(s) 321
BbsI GAAGAC 2 cut(s) 126, 340
Bbv12I GWGCWC 1 cut(s) 493
BccI CCATC 1 cut(s) 266
BciT130I CCWGG 1 cut(s) 319
BcnI CCSGG 2 cut(s) 116, 117
BfaI CTAG 2 cut(s) 24, 213
BisI GCNGC 1 cut(s) 210
BlsI GCNGC 1 cut(s) 211
BmcAI AGTACT 2 cut(s) 87, 435
Bme1390I CCNGG 3 cut(s) 116, 117, 319
BmeT110I CYCGRG 1 cut(s) 115
BmgT120I GGNCC 1 cut(s) 18
BmiI GGNNCC 1 cut(s) 323
BmrFI CCNGG 3 cut(s) 116, 117, 319
BmsI GCATC 1 cut(s) 296
BpiI GAAGAC 2 cut(s) 126, 340
BpuMI CCSGG 2 cut(s) 116, 117
BsaAI YACGTR 1 cut(s) 337
BsaJI CCNNGG 4 cut(s) 14, 115, 165, 366
BsaXI ACNNNNNCTCC 2 cut(s) 220, 250
Bse3DI GCAATG 1 cut(s) 174
BseBI CCWGG 1 cut(s) 319
BseDI CCNNGG 4 cut(s) 14, 115, 165, 366
BseGI GGATG 1 cut(s) 327
BseMI GCAATG 1 cut(s) 174
BshFI GGCC 3 cut(s) 20, 257, 365
BshNI GGYRCC 1 cut(s) 321
BsiHKAI GWGCWC 1 cut(s) 493
BsiHKCI CYCGRG 1 cut(s) 115
BsiSI CCGG 1 cut(s) 116
BsnI GGCC 3 cut(s) 20, 257, 365
BsoBI CYCGRG 1 cut(s) 115
Bsp1286I GDGCHC 1 cut(s) 493
Bsp143I GATC 1 cut(s) 339
Bsp19I CCATGG 2 cut(s) 14, 165
BspACI CCGC 2 cut(s) 201, 209
BspANI GGCC 3 cut(s) 20, 257, 365
BspLI GGNNCC 1 cut(s) 323
BspT107I GGYRCC 1 cut(s) 321
BsrDI GCAATG 1 cut(s) 174
BssECI CCNNGG 4 cut(s) 14, 115, 165, 366
BssMI GATC 1 cut(s) 339
BssT1I CCWWGG 2 cut(s) 14, 165
Bst2UI CCWGG 1 cut(s) 319
BstAPI GCANNNNNTGC 1 cut(s) 10
BstBAI YACGTR 1 cut(s) 337
BstC8I GCNNGC 2 cut(s) 67, 181
BstDEI CTNAG 1 cut(s) 218
BstDSI CCRYGG 2 cut(s) 14, 165
BstF5I GGATG 1 cut(s) 327
BstKTI GATC 1 cut(s) 342
BstMBI GATC 1 cut(s) 339
BstMWI GCNNNNNNNGC 2 cut(s) 10, 209
BstNI CCWGG 1 cut(s) 319
BstNSI RCATGY 1 cut(s) 69
BstSCI CCNGG 3 cut(s) 114, 115, 317
BstV2I GAAGAC 2 cut(s) 126, 340
BstXI CCANNNNNNTGG 1 cut(s) 166
BsuRI GGCC 3 cut(s) 20, 257, 365
BtgI CCRYGG 2 cut(s) 14, 165
BtsCI GGATG 1 cut(s) 327
BtsI GCAGTG 1 cut(s) 8
BtsIMutI CAGTG 1 cut(s) 8
Cac8I GCNNGC 2 cut(s) 67, 181
Cfr13I GGNCC 1 cut(s) 18
Cfr9I CCCGGG 1 cut(s) 115
Csp6I GTAC 3 cut(s) 86, 156, 434
CspCI CAANNNNNGTGG 2 cut(s) 151, 186
CviAII CATG 4 cut(s) 15, 66, 166, 361
CviJI RGCY 8 cut(s) 20, 183, 198, 212, 217, 257, 365, 505
CviKI_1 RGCY 8 cut(s) 20, 183, 198, 212, 217, 257, 365, 505
CviQI GTAC 3 cut(s) 86, 156, 434
DdeI CTNAG 1 cut(s) 218
DpnI GATC 1 cut(s) 341
DpnII GATC 1 cut(s) 339
EaeI YGGCCR 2 cut(s) 255, 363
Eco130I CCWWGG 2 cut(s) 14, 165
Eco57I CTGAAG 1 cut(s) 360
Eco88I CYCGRG 1 cut(s) 115
EcoRII CCWGG 1 cut(s) 317
EcoT14I CCWWGG 2 cut(s) 14, 165
ErhI CCWWGG 2 cut(s) 14, 165
FaeI CATG 4 cut(s) 18, 69, 169, 364
FaiI YATR 9 cut(s) 16, 67, 167, 263, 334, 362, 385, 387, 495
FatI CATG 4 cut(s) 14, 65, 165, 360
Fnu4HI GCNGC 1 cut(s) 210
FokI GGATG 1 cut(s) 314
Fsp4HI GCNGC 1 cut(s) 210
FspBI CTAG 2 cut(s) 24, 213
GluI GCNGC 1 cut(s) 210
HaeIII GGCC 3 cut(s) 20, 257, 365
HapII CCGG 1 cut(s) 116
Hin1II CATG 4 cut(s) 18, 69, 169, 364
HindIII AAGCTT 1 cut(s) 181
HinfI GANTC 2 cut(s) 222, 314
HpaII CCGG 1 cut(s) 116
Hpy188III TCNNGA 3 cut(s) 24, 76, 311
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 4 cut(s) 4, 69, 179, 287
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 209
HpyF3I CTNAG 1 cut(s) 218
HpySE526I ACGT 1 cut(s) 336
Hsp92II CATG 4 cut(s) 18, 69, 169, 364
Kzo9I GATC 1 cut(s) 339
LmnI GCTCC 1 cut(s) 203
LpnPI CCDG 5 cut(s) 89, 129, 304, 324, 331
LweI GCATC 1 cut(s) 296
MaeI CTAG 2 cut(s) 24, 213
MaeII ACGT 1 cut(s) 336
MaeIII GTNAC 1 cut(s) 413
MalI GATC 1 cut(s) 341
MboI GATC 1 cut(s) 339
MboII GAAGA 4 cut(s) 126, 334, 340, 366
MhlI GDGCHC 1 cut(s) 493
MlsI TGGCCA 1 cut(s) 257
MluCI AATT 3 cut(s) 33, 70, 99
MluNI TGGCCA 1 cut(s) 257
MlyI GAGTC 1 cut(s) 308
MnlI CCTC 2 cut(s) 31, 361
Mox20I TGGCCA 1 cut(s) 257
MscI TGGCCA 1 cut(s) 257
MslI CAYNNNNRTG 2 cut(s) 13, 164
Msp20I TGGCCA 1 cut(s) 257
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 1 cut(s) 116
MspR9I CCNGG 3 cut(s) 116, 117, 319
MvaI CCWGG 1 cut(s) 319
MwoI GCNNNNNNNGC 2 cut(s) 10, 209
NciI CCSGG 2 cut(s) 116, 117
NcoI CCATGG 2 cut(s) 14, 165
NdeII GATC 1 cut(s) 339
NlaIII CATG 4 cut(s) 18, 69, 169, 364
NlaIV GGNNCC 1 cut(s) 323
NmeAIII GCCGAG 1 cut(s) 391
NspI RCATGY 1 cut(s) 69
PaeI GCATGC 1 cut(s) 69
PcsI WCGNNNNNNNCGW 1 cut(s) 342
PfeI GAWTC 1 cut(s) 222
PkrI GCNGC 1 cut(s) 211
PleI GAGTC 1 cut(s) 308
PpsI GAGTC 1 cut(s) 308
Ppu21I YACGTR 1 cut(s) 337
Psp6I CCWGG 1 cut(s) 317
PspGI CCWGG 1 cut(s) 317
PspN4I GGNNCC 1 cut(s) 323
PspPI GGNCC 1 cut(s) 18
RsaI GTAC 3 cut(s) 87, 157, 435
RsaNI GTAC 3 cut(s) 86, 156, 434
RseI CAYNNNNRTG 2 cut(s) 13, 164
SatI GCNGC 1 cut(s) 210
Sau3AI GATC 1 cut(s) 339
Sau96I GGNCC 1 cut(s) 18
ScaI AGTACT 2 cut(s) 87, 435
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 3 cut(s) 116, 117, 319
SduI GDGCHC 1 cut(s) 493
SetI ASST 5 cut(s) 185, 200, 339, 372, 507
SfaNI GCATC 1 cut(s) 296
SmaI CCCGGG 1 cut(s) 117
SmiMI CAYNNNNRTG 2 cut(s) 13, 164
SphI GCATGC 1 cut(s) 69
Sse9I AATT 3 cut(s) 33, 70, 99
SsiI CCGC 2 cut(s) 201, 209
SspI AATATT 1 cut(s) 55
SspMI CTAG 2 cut(s) 24, 213
StyD4I CCNGG 3 cut(s) 114, 115, 317
StyI CCWWGG 2 cut(s) 14, 165
TaiI ACGT 1 cut(s) 339
TasI AATT 3 cut(s) 33, 70, 99
TatI WGTACW 2 cut(s) 85, 433
TauI GCSGC 1 cut(s) 212
TfiI GAWTC 1 cut(s) 222
TscAI CASTG 1 cut(s) 15
TspDTI ATGAA 1 cut(s) 349
TspMI CCCGGG 1 cut(s) 115
TspRI CASTG 1 cut(s) 15
XbaI TCTAGA 1 cut(s) 23
XceI RCATGY 1 cut(s) 69
XcmI CCANNNNNNNNNTGG 1 cut(s) 250
XmaI CCCGGG 1 cut(s) 115
XspI CTAG 2 cut(s) 24, 213
ZrmI AGTACT 2 cut(s) 87, 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.