pycom15g08080

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000265
Physical Location & Seq
Forward (+)
5001304 .. 5003671
2368 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1248 bp
ATGCCTTCGTTTTCTCAAATGGAAAGAGCAAGATCATTTCTTCAGTCTCCCACTTATGGAAACCTCGTCACTGTCCTTAGCATTGACGGAGGTGGTATCCGAGGGATTATCCCCGGAACTATACTCAGCTTCCTAGAGTCTGAGCTTCAGAAGCTGGATGGTGAAGATGCAAGGCTGGCAGATTACTTTGATGTAATTACAGGGACTAGCACAGGTGGACTTGTGACTGCCATGCTTACTACCCCAGATGAAAATAATCGTCCCTTGTTTTCTGCTAAAGATATTAACGCTTTCTACCTCGAACACTGCCCGAAAATCTTCCCCCAGGGCAGCGCTCCACTAATTGGTGAAGCCGTGGATATGGTTAAAAATTTGACAGGACCAAAATATGATGGGAAATATTTACATAATATAGTGAAGGAAAAATTAGGAGACAAACGATTGAACGACACATTGACTAACGTTATAATCCCAACTTTTGATATAAAGCGGCTCCAACCAACCATCTTTTCCAGCTATGAGGCCAAGAAGAAGAGTTGCGTAGATGCTTTACTCTCGGACATATGTATTGCAACTTCAGCAGCACCAACCTACCTTCCAGCACATCATTTTGAAACCAAAACTTCCACTGGTGAAACAAGAGAGTTCCATCTCATTGATGGTGGTGTCGCTGCTAATAACCCGGCATTGGTTGCCATAAACGAAGTCACGAAGGAAATCCACCAGGGAAATTCCGACTTCAACTTTTCCACGAGACCAACTGGGTATGGTCGGTTCTTAGTTGTATCGTTGGGAACTGGTTCTGCAAAATGTGAGGAGAAGTACCATGCCAATGACGTAGCTAAATGGGGTCTTTTGGCATGGTTGACAAGCGGAGGTTCTTGTCCACTCACCGATGTATTTACCCAAGCCAGCAGTGACATGGTTGATTTTCACCTTGCCACTGTTTTCCAAGCTCTTAACTGTGAGAAAAGTTATCTTCGAATCCAGGATGATACATTGGAAAAGACAGTGTCTTCAGTAGACATTGCTACGAAGGAAAACTTGGATAATCTTGTGAAAGTCGGAGAGAAATTGCTGAAAAAGCCTGTTTCAAGGGTGAATATGGATACCGGTGTCTTTGAACCTGCCCATCAATCTACAAATGAAGAGGCTCTCGTAAGGATTGCTGGAATTCTGTCTCGGGAGAGGAAGGTCCGTGAAACGCAATCACCACATGGAAAGGCCGTAACCAACAAGTCCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

416

Amino Acids

45.37

Weight (kDa)

5.97

Isoelectric Point (pI)

29.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 26 - 233 1.3e-26 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 467
Acc36I ACCTGC 1 cut(s) 1135
AccB7I CCANNNNNTGG 1 cut(s) 344
AccI GTMKAC 1 cut(s) 1023
AciI CCGC 2 cut(s) 490, 873
AclI AACGTT 1 cut(s) 462
AcsI RAATTY 3 cut(s) 370, 730, 1173
AcuI CTGAAG 4 cut(s) 26, 131, 561, 1002
AfaI GTAC 1 cut(s) 824
AfeI AGCGCT 1 cut(s) 334
AfiI CCNNNNNNNGG 3 cut(s) 56, 344, 688
AgeI ACCGGT 1 cut(s) 1112
AgsI TTSAA 5 cut(s) 445, 614, 742, 1095, 1124
AjnI CCWGG 3 cut(s) 324, 723, 987
AjuI GAANNNNNNNTTGG 4 cut(s) 493, 525, 1028, 1060
AluBI AGCT 6 cut(s) 129, 145, 154, 516, 842, 956
AluI AGCT 6 cut(s) 129, 145, 154, 516, 842, 956
Alw26I GTCTC 4 cut(s) 51, 426, 748, 1185
AlwNI CAGNNNCTG 1 cut(s) 154
Ama87I CYCGRG 1 cut(s) 1182
Aor51HI AGCGCT 1 cut(s) 334
AoxI GGCC 2 cut(s) 522, 1224
ApeKI GCWGC 3 cut(s) 330, 581, 671
ApoI RAATTY 3 cut(s) 370, 730, 1173
AsiGI ACCGGT 1 cut(s) 1112
Asp700I GAANNNNTTC 2 cut(s) 317, 799
AspLEI GCGC 1 cut(s) 335
AspS9I GGNCC 2 cut(s) 380, 1195
AsuC2I CCSGG 2 cut(s) 114, 683
AsuHPI GGTGA 7 cut(s) 173, 359, 644, 883, 926, 1111, 1203
AsuII TTCGAA 1 cut(s) 982
AvaI CYCGRG 1 cut(s) 1182
AvaII GGWCC 2 cut(s) 380, 1195
BaeI ACNNNNGTAYC 2 cut(s) 1101, 1134
BarI GAAGNNNNNNTAC 2 cut(s) 559, 591
BauI CACGAG 1 cut(s) 751
BbsI GAAGAC 1 cut(s) 1008
BbvI GCAGC 3 cut(s) 342, 593, 658
BccI CCATC 6 cut(s) 152, 386, 512, 653, 657, 1140
BceAI ACGGC 2 cut(s) 338, 1211
BciT130I CCWGG 3 cut(s) 326, 725, 989
BciVI GTATCC 2 cut(s) 107, 1102
BcnI CCSGG 2 cut(s) 114, 683
BcoDI GTCTC 4 cut(s) 51, 426, 748, 1185
BfaI CTAG 2 cut(s) 134, 207
BfoI RGCGCY 1 cut(s) 336
BfuAI ACCTGC 1 cut(s) 1135
BfuI GTATCC 2 cut(s) 107, 1102
BisI GCNGC 4 cut(s) 331, 491, 582, 672
BlsI GCNGC 4 cut(s) 332, 492, 583, 673
Bme1390I CCNGG 5 cut(s) 114, 326, 683, 725, 989
Bme18I GGWCC 2 cut(s) 380, 1195
BmeT110I CYCGRG 1 cut(s) 1182
BmgT120I GGNCC 2 cut(s) 380, 1195
BmiI GGNNCC 1 cut(s) 494
BmrFI CCNGG 5 cut(s) 114, 326, 683, 725, 989
BmrI ACTGGG 1 cut(s) 771
BmsI GCATC 2 cut(s) 157, 535
BmuI ACTGGG 1 cut(s) 771
BpiI GAAGAC 1 cut(s) 1008
Bpu10I CCTNAGC 1 cut(s) 77
Bpu14I TTCGAA 1 cut(s) 982
BpuMI CCSGG 2 cut(s) 114, 683
BsaI GGTCTC 1 cut(s) 748
BsaJI CCNNGG 6 cut(s) 100, 112, 324, 325, 354, 724
BsaWI WCCGGW 1 cut(s) 1112
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 344, 688
Bse118I RCCGGY 1 cut(s) 1112
Bse1I ACTGG 3 cut(s) 634, 766, 802
Bse3DI GCAATG 1 cut(s) 1026
BseBI CCWGG 3 cut(s) 326, 725, 989
BseDI CCNNGG 6 cut(s) 100, 112, 324, 325, 354, 724
BseGI GGATG 2 cut(s) 163, 997
BseLI CCNNNNNNNGG 3 cut(s) 56, 344, 688
BseMI GCAATG 1 cut(s) 1026
BseMII CTCAG 2 cut(s) 132, 139
BseNI ACTGG 3 cut(s) 634, 766, 802
BseRI GAGGAG 1 cut(s) 830
BseXI GCAGC 3 cut(s) 342, 593, 658
BshFI GGCC 2 cut(s) 524, 1226
BshTI ACCGGT 1 cut(s) 1112
BsiHKCI CYCGRG 1 cut(s) 1182
BsiSI CCGG 3 cut(s) 114, 683, 1113
BslFI GGGAC 2 cut(s) 217, 246
BslI CCNNNNNNNGG 3 cut(s) 56, 344, 688
BsmAI GTCTC 4 cut(s) 51, 426, 748, 1185
BsmFI GGGAC 2 cut(s) 217, 246
BsnI GGCC 2 cut(s) 524, 1226
Bso31I GGTCTC 1 cut(s) 748
BsoBI CYCGRG 1 cut(s) 1182
Bsp119I TTCGAA 1 cut(s) 982
Bsp143I GATC 1 cut(s) 32
BspACI CCGC 2 cut(s) 490, 873
BspANI GGCC 2 cut(s) 524, 1226
BspCNI CTCAG 2 cut(s) 133, 138
BspLI GGNNCC 1 cut(s) 494
BspMI ACCTGC 1 cut(s) 1135
BspT104I TTCGAA 1 cut(s) 982
BspTNI GGTCTC 1 cut(s) 748
BsrDI GCAATG 1 cut(s) 1026
BsrFI RCCGGY 1 cut(s) 1112
BsrI ACTGG 3 cut(s) 634, 766, 802
BssAI RCCGGY 1 cut(s) 1112
BssECI CCNNGG 6 cut(s) 100, 112, 324, 325, 354, 724
BssMI GATC 1 cut(s) 32
BssSI CACGAG 1 cut(s) 751
Bst2BI CACGAG 1 cut(s) 751
Bst2UI CCWGG 3 cut(s) 326, 725, 989
Bst4CI ACNGT 4 cut(s) 73, 946, 965, 1012
Bst6I CTCTTC 2 cut(s) 527, 1143
BstAPI GCANNNNNTGC 1 cut(s) 692
BstBI TTCGAA 1 cut(s) 982
BstC8I GCNNGC 2 cut(s) 177, 913
BstDEI CTNAG 4 cut(s) 77, 125, 141, 778
BstDSI CCRYGG 1 cut(s) 354
BstF5I GGATG 2 cut(s) 163, 997
BstH2I RGCGCY 1 cut(s) 336
BstHHI GCGC 1 cut(s) 335
BstKTI GATC 1 cut(s) 35
BstMAI GTCTC 4 cut(s) 51, 426, 748, 1185
BstMBI GATC 1 cut(s) 32
BstMWI GCNNNNNNNGC 5 cut(s) 151, 176, 578, 692, 1084
BstNI CCWGG 3 cut(s) 326, 725, 989
BstSCI CCNGG 5 cut(s) 112, 324, 681, 723, 987
BstV1I GCAGC 3 cut(s) 342, 593, 658
BstV2I GAAGAC 1 cut(s) 1008
BsuI GTATCC 2 cut(s) 107, 1102
BsuRI GGCC 2 cut(s) 524, 1226
BtgI CCRYGG 1 cut(s) 354
BtsCI GGATG 2 cut(s) 163, 997
BtsI GCAGTG 2 cut(s) 304, 922
BtsIMutI CAGTG 6 cut(s) 69, 304, 627, 922, 942, 1017
BveI ACCTGC 1 cut(s) 1135
Cac8I GCNNGC 2 cut(s) 177, 913
CaiI CAGNNNCTG 1 cut(s) 154
CfoI GCGC 1 cut(s) 335
Cfr10I RCCGGY 1 cut(s) 1112
Cfr13I GGNCC 2 cut(s) 380, 1195
Csp6I GTAC 1 cut(s) 823
CspAI ACCGGT 1 cut(s) 1112
CviAII CATG 5 cut(s) 232, 827, 861, 922, 1217
CviQI GTAC 1 cut(s) 823
DdeI CTNAG 4 cut(s) 77, 125, 141, 778
DpnI GATC 1 cut(s) 34
DpnII GATC 1 cut(s) 32
Eam1104I CTCTTC 2 cut(s) 527, 1143
EarI CTCTTC 2 cut(s) 527, 1143
Eco31I GGTCTC 1 cut(s) 748
Eco47I GGWCC 2 cut(s) 380, 1195
Eco47III AGCGCT 1 cut(s) 334
Eco57I CTGAAG 4 cut(s) 26, 131, 561, 1002
Eco88I CYCGRG 1 cut(s) 1182
EcoRI GAATTC 1 cut(s) 1173
EcoRII CCWGG 3 cut(s) 324, 723, 987
FaeI CATG 5 cut(s) 235, 830, 864, 925, 1220
FalI AAGNNNNNCTT 2 cut(s) 1028, 1060
FaqI GGGAC 2 cut(s) 217, 246
FatI CATG 5 cut(s) 231, 826, 860, 921, 1216
FauNDI CATATG 1 cut(s) 563
FblI GTMKAC 1 cut(s) 1023
Fnu4HI GCNGC 4 cut(s) 331, 491, 582, 672
FokI GGATG 2 cut(s) 170, 1004
Fsp4HI GCNGC 4 cut(s) 331, 491, 582, 672
FspBI CTAG 2 cut(s) 134, 207
GlaI GCGC 1 cut(s) 334
GluI GCNGC 4 cut(s) 331, 491, 582, 672
HaeII RGCGCY 1 cut(s) 336
HaeIII GGCC 2 cut(s) 524, 1226
HapII CCGG 3 cut(s) 114, 683, 1113
HhaI GCGC 1 cut(s) 335
Hin1II CATG 5 cut(s) 235, 830, 864, 925, 1220
Hin6I GCGC 1 cut(s) 333
HinP1I GCGC 1 cut(s) 333
HincII GTYRAC 1 cut(s) 867
HindII GTYRAC 1 cut(s) 867
HinfI GANTC 2 cut(s) 137, 984
HpaII CCGG 3 cut(s) 114, 683, 1113
HphI GGTGA 7 cut(s) 173, 359, 644, 883, 926, 1111, 1203
Hpy166II GTNNAC 4 cut(s) 218, 867, 887, 1024
Hpy188I TCNGA 6 cut(s) 101, 142, 150, 559, 736, 1067
Hpy188III TCNNGA 2 cut(s) 709, 1184
Hpy8I GTNNAC 4 cut(s) 218, 867, 887, 1024
HpyAV CCTTC 6 cut(s) 15, 412, 605, 706, 1030, 1186
HpyCH4III ACNGT 4 cut(s) 73, 946, 965, 1012
HpyCH4IV ACGT 2 cut(s) 462, 837
HpyCH4V TGCA 3 cut(s) 170, 572, 806
HpyF10VI GCNNNNNNNGC 5 cut(s) 151, 176, 578, 692, 1084
HpyF3I CTNAG 4 cut(s) 77, 125, 141, 778
HpySE526I ACGT 2 cut(s) 462, 837
Hsp92II CATG 5 cut(s) 235, 830, 864, 925, 1220
HspAI GCGC 1 cut(s) 333
Kzo9I GATC 1 cut(s) 32
LmnI GCTCC 2 cut(s) 340, 498
Lsp1109I GCAGC 3 cut(s) 342, 593, 658
LweI GCATC 2 cut(s) 157, 535
MaeI CTAG 2 cut(s) 134, 207
MaeII ACGT 2 cut(s) 462, 837
MaeIII GTNAC 5 cut(s) 67, 223, 706, 917, 1228
MalI GATC 1 cut(s) 34
MboI GATC 1 cut(s) 32
MboII GAAGA 8 cut(s) 32, 176, 310, 541, 544, 971, 1008, 1160
MluCI AATT 7 cut(s) 195, 342, 370, 425, 730, 1073, 1173
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 3 cut(s) 520, 759, 1045
MnlI CCTC 9 cut(s) 74, 83, 95, 308, 514, 808, 869, 1144, 1182
MroXI GAANNNNTTC 2 cut(s) 317, 799
MseI TTAA 3 cut(s) 285, 366, 960
MslI CAYNNNNRTG 1 cut(s) 831
MspI CCGG 3 cut(s) 114, 683, 1113
MspR9I CCNGG 5 cut(s) 114, 326, 683, 725, 989
MvaI CCWGG 3 cut(s) 326, 725, 989
MwoI GCNNNNNNNGC 5 cut(s) 151, 176, 578, 692, 1084
NciI CCSGG 2 cut(s) 114, 683
NdeI CATATG 1 cut(s) 563
NdeII GATC 1 cut(s) 32
NlaIII CATG 5 cut(s) 235, 830, 864, 925, 1220
NlaIV GGNNCC 1 cut(s) 494
NmuCI GTSAC 4 cut(s) 67, 223, 706, 917
NspV TTCGAA 1 cut(s) 982
PasI CCCWGGG 1 cut(s) 325
PdmI GAANNNNTTC 2 cut(s) 317, 799
PfeI GAWTC 1 cut(s) 984
PflFI GACNNNGTC 1 cut(s) 1012
PflMI CCANNNNNTGG 1 cut(s) 344
PfoI TCCNGGA 1 cut(s) 987
PinAI ACCGGT 1 cut(s) 1112
PkrI GCNGC 4 cut(s) 332, 492, 583, 673
PleI GAGTC 1 cut(s) 145
PpsI GAGTC 1 cut(s) 145
PsiI TTATAA 1 cut(s) 467
Psp1406I AACGTT 1 cut(s) 462
Psp6I CCWGG 3 cut(s) 324, 723, 987
PspGI CCWGG 3 cut(s) 324, 723, 987
PspN4I GGNNCC 1 cut(s) 494
PspPI GGNCC 2 cut(s) 380, 1195
PsrI GAACNNNNNNTAC 2 cut(s) 758, 790
PstNI CAGNNNCTG 1 cut(s) 154
PsyI GACNNNGTC 1 cut(s) 1012
RsaI GTAC 1 cut(s) 824
RsaNI GTAC 1 cut(s) 823
RseI CAYNNNNRTG 1 cut(s) 831
SaqAI TTAA 3 cut(s) 285, 366, 960
SatI GCNGC 4 cut(s) 331, 491, 582, 672
Sau3AI GATC 1 cut(s) 32
Sau96I GGNCC 2 cut(s) 380, 1195
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 5 cut(s) 114, 326, 683, 725, 989
SfaNI GCATC 2 cut(s) 157, 535
SfuI TTCGAA 1 cut(s) 982
SinI GGWCC 2 cut(s) 380, 1195
SmiMI CAYNNNNRTG 1 cut(s) 831
Sse9I AATT 7 cut(s) 195, 342, 370, 425, 730, 1073, 1173
SsiI CCGC 2 cut(s) 490, 873
SspI AATATT 1 cut(s) 401
SspMI CTAG 2 cut(s) 134, 207
StyD4I CCNGG 5 cut(s) 112, 324, 681, 723, 987
TaaI ACNGT 4 cut(s) 73, 946, 965, 1012
TaiI ACGT 2 cut(s) 465, 840
TaqI TCGA 2 cut(s) 300, 982
TasI AATT 7 cut(s) 195, 342, 370, 425, 730, 1073, 1173
TauI GCSGC 1 cut(s) 493
TfiI GAWTC 1 cut(s) 984
Tru1I TTAA 3 cut(s) 285, 366, 960
Tru9I TTAA 3 cut(s) 285, 366, 960
TscAI CASTG 6 cut(s) 76, 311, 634, 922, 949, 1017
TseFI GTSAC 4 cut(s) 67, 223, 706, 917
TseI GCWGC 3 cut(s) 330, 581, 671
Tsp45I GTSAC 4 cut(s) 67, 223, 706, 917
TspDTI ATGAA 2 cut(s) 264, 1161
TspGWI ACGGA 2 cut(s) 102, 1187
TspRI CASTG 6 cut(s) 76, 311, 634, 922, 949, 1017
Tth111I GACNNNGTC 1 cut(s) 1012
Van91I CCANNNNNTGG 1 cut(s) 344
VpaK11BI GGWCC 2 cut(s) 380, 1195
XapI RAATTY 3 cut(s) 370, 730, 1173
XcmI CCANNNNNNNNNTGG 2 cut(s) 656, 919
XmiI GTMKAC 1 cut(s) 1023
XmnI GAANNNNTTC 2 cut(s) 317, 799
XspI CTAG 2 cut(s) 134, 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.