RchiOBHm_Chr5g0029771

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000292
Physical Location & Seq
Reverse (-)
23534431 .. 23543936
9506 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30913

Sequence Viewer

Length: 2856 bp
ATGTTTGAGGATGGTGTTTGTTTAGAAGAATTAAATGGTTGCATTTTACTGATTGTAGTACAAGCTTTAAGGGACATTGGAAAGACACTCGGTAAGAACTGGAATTTCAGCGTAGATCCATGCAGCGGAGAACTTGGATGGAGTACTGCTCCGGATGGCCAATACATGAATAACGTTACCTGCGGCAACTGTAGTTCTACCTCCAATACTACGACCGATCAAATCTGCCATGTCACAAACATACTTTTGAAAGGGCAGAATCTGACGGGACAACTCCCCAAGGAGTTGATCAACCTCTCCTATCTCCAAGAAATTGACCTCACCCGCAACTTCCTGAATGGCACCATCCCTCCAGAATGGGGCACCCTTCCACTAGTTAGCATTTCTCTTGTTGGAAACCGTTTGACAGGTTCTATCCCTGTTGAGCTAGGAAACATCACCACGCTCAAAAGTTTGACAATCGATTTCAATCGTTTATCTGGAGTTCTTCCTGCGGAGCTTGGAAATTTGTCTAGCATAGAAAGAATGCTTCTTACCTCGAACAATTTTACTGGGGAGTTGCCAGAAACATTTGCAAAGCTTAACACCTTAACTGACTTTCGGATTAGTGACAGTTCCTTTTCTGGGAAGATACCTGATTTTATTCAGAGCTGGACAAATCTTGAGGGACTAGTGATTCAGGCTAGTGGTTTTACAGGGCCAATTCCTTCCGGCATTTCTCTCTTGACAAGATTAACTGACCTGGTGTTGCGGAACTGCAATATTAGTGGGCAGCTACCTGAATATGTTGGGAACATGTCGACTTTAAAAATTTTAGACCTTAGTTTTAACAAGCTAACTGGAGATATCCCAAACAACTTTGCTGGTCTAGGAAATCTGGATAATATGTTCTTAACTGGAAACATGCTGACCGGAACTGTGCCAGCTGATTGGATGAAAGCCAATAGCATATTTGTTTCATGTTTACCACTTGCAAAATGTGTAAAAACTTGGTACACTCTTCATATAAATTGTGGTGGAAAAGAAGTACCCCTCTATGAAAATTTCACAAGACCCACCAACGCAACATACGAGGAAGATACTGATGTAGCCGGGCCTTCATCAGTTTACCTGAGCAGGACAAATTGGGCATCAAGCACGACTGGCTACTTCGCTGATGATAATCGTCTTACTAAAACCTATACCTGGACTAATTCATCTGGACTCAATATGGCGAATCCTGAGTTGTACATGAATGCACACCTTTCTCCTATTTCTCTAACTTACTATGGGTTTTGTCTCGGAAATGGAAGCTACACAGTAAACCTCCACTTTGCAGAGATAATATTTACAAATGACACAACATATAGTAGCCTGGGAAGACGTGTATTCGATATATACATTCAGGGGTCACTAGCGTTCAAGGATTTCAATATTGCAGATGCTGCAGGTGGCTTTGGAAACGCAGTCACAAAAAAGTTCACTGCTAACGTAACTGCTGGTACTTTGGAGATCCGTTTATTCTGGGCTGGGAAAGGGACAACAGGTGTTCCTTACAGAGGAGACTATGGTCCTCTTATATCAGCTATCTCCATAGATTCTGACTTTCCACCCCCTCAAGAAACCCAGCCAGGAGGTGGAAGCAGCGGTATATCAGCAGGTGCAGTGGTTGGAATCGTGGCGGGAGGAGTCTTCATTATATTACTGGTTTTAGGAATTCTCTGGTGGAAAGGGTTCATAGGACCAGCAAATACTTTAGAACAAGATTTGAAGGGTGTGGACCTGCAAACCAGCAGATTTACCTTGAGGCAAATCAAAGTTGCCACAAACAACTTTGACATATCAAATAAGATTGGAGAAGGTGGTTTTGGTCCTGTTTACAAGGGCCTTCTATCAGATGACACCGCAATTGCTGTTAAGAAGCTTTCTGCCAAATCAAAGCAAGGGAATCGTGAATTTGTGAATGAGATTGGCATGATTTCTGCTCTGCAACACCCTTACCTTGTCAAGCTTTATGGTTGTTGTATTGAAGGAAATGACTTGTTGCTTGTCTATGAGTACTTGGAAAATAATAGCGTTGCTCGTGCTCTGTTTGGCCCAAAAGCAAGTCAGGTGAAGATGGATTGGCCAACAAGGCACAGGATTTGTGTTGGTATAGCAAAAGGTTTGGCTTACCTCCATGAGGAATCAAGGTTGAAGATCGTTCATAGAGACATTAAAGCTACTAATGTGCTACTCGATAAAAATCTTTGTCCAAAGATAGCTGATTTTGGATTGGCCAAGCTTGATGAACAGGATAATACCCACATAAGCACCCGGATTGCTGGAACTCTAGGATATATGGCACCTGAATATGCAATGCGAGGTTATCTGACTGATAAAGCAGATGTTTATAGTTTCGGAATTGTTGTGTTGGAAATAGTCAGTGGGAGGAGTAACACAACTTACCGCTCTGAAGAAGAATGTTTTTATCTTCTTGATTGGGCACTTCTTCTAAAGGAGAGAGGGAGTTTGTTGGACCTAGTGGATCCAAGGCTAGGCTCACAGTTTAACAAAGAGCAGATGATTACTACAATCAATGTGGCTCTCCTTTGCTCTCATGTGTCTTCGGCAGTTAGGCCTTCCATGTCTTCAGTGGTGAAAATGCTTGAAGGCAGGGCTTCGGTTCAGGAGGTGATCTCTGATCCAAATGCCTCAAACAATGAAATCAATGCAATGAGGAAACATTTTCAATCCAGTTTCGAAGAAAACAATGGTGACCTTGAGAGTCAGAGACAAACTATGTCAATTGAACCGTCGTGGACTGCTTCATCTACATCTGCTCATGATCTCTATCTCGTCCATCCTGATTCCAGTTATTGGGAGAACAGAAAATAG

Protein Analysis

951

Amino Acids

104.11

Weight (kDa)

5.35

Isoelectric Point (pI)

28.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 244 - 303 2.2e-08 Leucine rich repeat
Malectin PF11721 333 - 523 2.1e-39 Malectin domain
Pkinase PF00069 606 - 808 6.5e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 607 - 812 5.5e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000292)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53420 AT3G14840
fragaria_vesca FvH4_3g17840 FvH4_3g17840 FvH4_3g17840 FvH4_3g17840 FvH4_3g17840 FvH4_3g17840 FvH4_3g17841 FvH4_3g17841 FvH4_3g17841 FvH4_3g17841 FvH4_3g17841 FvH4_3g17841 FvH4_3g17850 FvH4_3g17850 FvH4_3g17850 FvH4_3g17850 FvH4_3g17851
malus_domestica MD03G1246600.v1.1 MD06G1045200.v1.1 MD06G1045400.v1.1 MD11G1267800.v1.1 MD11G1267900.v1.1 MD11G1268100.v1.1 MD11G1268300.v1.1 MD11G1268500.v1.1 MD11G1268600.v1.1
prunus_persica Prupe.4G157700_v2.0.a1 Prupe.4G157700_v2.0.a1 Prupe.4G157800_v2.0.a1 Prupe.4G157800_v2.0.a1 Prupe.4G157800_v2.0.a1 Prupe.4G157800_v2.0.a1 Prupe.4G157800_v2.0.a1 Prupe.4G157900_v2.0.a1 Prupe.4G157900_v2.0.a1 Prupe.4G157900_v2.0.a1
pyrus_communis pycom03g19540 pycom11g23600 pycom11g23630 pycom11g23690 pycom11g23720 pycom11g23730 pycom11g23740 pycom11g23750
rosa_chinensis RchiOBHm_Chr0c40g0503411 RchiOBHm_Chr0c40g0503421 RchiOBHm_Chr5g0029631 RchiOBHm_Chr5g0029661 RchiOBHm_Chr5g0029701 RchiOBHm_Chr5g0029741 RchiOBHm_Chr5g0029771 RchiOBHm_Chr5g0029781 RchiOBHm_Chr5g0029811 RchiOBHm_Chr5g0029851 RchiOBHm_Chr5g0029871 RchiOBHm_Chr5g0029891 RchiOBHm_Chr7g0222221
rosa_laevigata RLG00000017380 RLG00000032873 RLG00000033188 RLG00000033190 RLG00000033192 RLG00000033193 RLG00000033194 RLG00000033196 RLG00000033199 RLG00000033238
rosa_multiflora Rmu_co8359987.1_g000001 Rmu_co8444909.1_g000001 Rmu_sc0001608.1_g000007 Rmu_sc0001608.1_g000009 Rmu_sc0001608.1_g000013 Rmu_sc0001608.1_g000018 Rmu_sc0001608.1_g000023 Rmu_sc0001608.1_g000040 Rmu_sc0003238.1_g000016 Rmu_sc0003368.1_g000026 Rmu_sc0003368.1_g000034 Rmu_sc0003368.1_g000043 Rmu_sc0003368.1_g000047 Rmu_sc0003368.1_g000052 Rmu_sc0003368.1_g000054 Rmu_sc0004963.1_g000001
rosa_roxburghii Rroxscaffold_1G00050080 Rroxscaffold_1G00050090 Rroxscaffold_1G00050110 Rroxscaffold_1G00050120 Rroxscaffold_1G00050180 Rroxscaffold_1G00050190 Rroxscaffold_1G00050220 Rroxscaffold_1G00050270 Rroxscaffold_1G00050300
rosa_rugosa Rorug04G0065200 Rorug05G0114900 Rorug05G0115000 Rorug05G0115100 Rorug05G0120100 Rorug05G0238900
rosa_samantha Rh5AG207800 Rh5AG208000 Rh5AG208300 Rh5AG208400 Rh5AG208800 Rh5AG294900 Rh5AG295000 Rh5AG295400 Rh5CG230400 Rh7AG347800
rosa_wichuraiana Rw0G005370 Rw0G012460 Rw0G019060 Rw5G018910 Rw5G018920 Rw5G018930 Rw5G018940 Rw5G018960 Rw5G018970 Rw5G018980 Rw5G018990 Rw5G019000 Rw7G029840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1418, 1628
Acc36I ACCTGC 4 cut(s) 188, 1418, 1628, 1770
AccB1I GGYRCC 3 cut(s) 341, 362, 2323
AccB7I CCANNNNNTGG 2 cut(s) 1616, 2838
AccBSI CCGCTC 1 cut(s) 2430
AccI GTMKAC 1 cut(s) 800
AccIII TCCGGA 1 cut(s) 151
AciI CCGC 9 cut(s) 126, 183, 325, 494, 751, 1626, 1661, 1884, 2428
AclI AACGTT 1 cut(s) 174
AclWI GGATC 5 cut(s) 110, 1486, 2501, 2514, 2657
AcoI YGGCCR 3 cut(s) 157, 2105, 2256
AcsI RAATTY 6 cut(s) 103, 505, 810, 1042, 1695, 1934
AcuI CTGAAG 2 cut(s) 2454, 2595
AfaI GTAC 7 cut(s) 60, 145, 995, 1029, 1229, 1483, 2039
AfiI CCNNNNNNNGG 9 cut(s) 125, 359, 624, 1185, 1538, 1616, 2161, 2516, 2838
AflIII ACRYGT 2 cut(s) 795, 1363
AhlI ACTAGT 2 cut(s) 373, 670
AjiI CACGTC 1 cut(s) 1364
AjnI CCWGG 4 cut(s) 741, 1184, 1353, 1609
AjuI GAANNNNNNNTTGG 2 cut(s) 1830, 1862
AloI GAACNNNNNNTCC 2 cut(s) 872, 904
Alw21I GWGCWC 1 cut(s) 2068
Alw26I GTCTC 4 cut(s) 1283, 1536, 2184, 2746
AlwI GGATC 5 cut(s) 110, 1486, 2501, 2514, 2657
AlwNI CAGNNNCTG 2 cut(s) 262, 1424
Aor13HI TCCGGA 1 cut(s) 151
AoxI GGCC 8 cut(s) 157, 698, 1094, 1864, 2074, 2105, 2256, 2597
ApeKI GCWGC 4 cut(s) 123, 772, 1424, 1623
ApoI RAATTY 6 cut(s) 103, 505, 810, 1042, 1695, 1934
Asp700I GAANNNNTTC 1 cut(s) 1712
AspS9I GGNCC 9 cut(s) 698, 1094, 1550, 1721, 1759, 1850, 1864, 2075, 2497
AsuC2I CCSGG 2 cut(s) 1093, 2296
AsuHPI GGTGA 6 cut(s) 313, 430, 2104, 2629, 2665, 2747
AsuII TTCGAA 1 cut(s) 2721
AvaII GGWCC 5 cut(s) 1550, 1721, 1759, 1850, 2497
BaeGI GKGCMC 2 cut(s) 365, 2467
BaeI ACNNNNGTAYC 2 cut(s) 1071, 1104
BalI TGGCCA 3 cut(s) 159, 2107, 2258
BamHI GGATCC 1 cut(s) 2506
BanI GGYRCC 3 cut(s) 341, 362, 2323
BauI CACGAG 1 cut(s) 2061
BbsI GAAGAC 4 cut(s) 1366, 1663, 2577, 2601
Bbv12I GWGCWC 1 cut(s) 2068
BbvI GCAGC 4 cut(s) 135, 784, 1411, 1635
BccI CCATC 6 cut(s) 5, 132, 149, 353, 2092, 2829
BcgI CGANNNNNNTGC 2 cut(s) 1910, 1944
BciT130I CCWGG 4 cut(s) 743, 1186, 1355, 1611
BclI TGATCA 1 cut(s) 288
BcnI CCSGG 2 cut(s) 1093, 2296
BcoDI GTCTC 4 cut(s) 1283, 1536, 2184, 2746
BcuI ACTAGT 2 cut(s) 373, 670
BfmI CTRYAG 2 cut(s) 190, 1425
BfuAI ACCTGC 4 cut(s) 188, 1418, 1628, 1770
BglI GCCNNNNNGGC 1 cut(s) 2113
BisI GCNGC 5 cut(s) 124, 184, 773, 1425, 1624
BlsI GCNGC 5 cut(s) 125, 185, 774, 1426, 1625
BmcAI AGTACT 2 cut(s) 145, 2039
Bme1390I CCNGG 6 cut(s) 743, 1093, 1186, 1355, 1611, 2296
Bme18I GGWCC 5 cut(s) 1550, 1721, 1759, 1850, 2497
BmgBI CACGTC 1 cut(s) 1364
BmgT120I GGNCC 9 cut(s) 698, 1094, 1550, 1721, 1759, 1850, 1864, 2075, 2497
BmiI GGNNCC 4 cut(s) 343, 364, 2325, 2508
BmrFI CCNGG 6 cut(s) 743, 1093, 1186, 1355, 1611, 2296
BmrI ACTGGG 1 cut(s) 561
BmsI GCATC 2 cut(s) 1139, 1411
BmuI ACTGGG 1 cut(s) 561
BoxI GACNNNNGTC 1 cut(s) 1548
BpiI GAAGAC 4 cut(s) 1366, 1663, 2577, 2601
BplI GAGNNNNNCTC 4 cut(s) 133, 165, 2642, 2674
BpmI CTGGAG 3 cut(s) 336, 501, 861
Bpu10I CCTNAGC 1 cut(s) 1112
Bpu14I TTCGAA 1 cut(s) 2721
BpuEI CTTGAG 4 cut(s) 683, 1581, 1804, 2762
BpuMI CCSGG 2 cut(s) 1093, 2296
Bsa29I ATCGAT 1 cut(s) 462
BsaBI GATNNNNATC 4 cut(s) 468, 1161, 2223, 2811
BsaJI CCNNGG 3 cut(s) 279, 1354, 2510
BsaWI WCCGGW 2 cut(s) 151, 911
BsaXI ACNNNNNCTCC 2 cut(s) 334, 364
Bsc4I CCNNNNNNNGG 9 cut(s) 125, 359, 624, 1185, 1538, 1616, 2161, 2516, 2838
Bse1I ACTGG 8 cut(s) 104, 556, 844, 901, 1147, 1689, 2715, 2832
Bse3DI GCAATG 2 cut(s) 2343, 2700
Bse8I GATNNNNATC 4 cut(s) 468, 1161, 2223, 2811
BseAI TCCGGA 1 cut(s) 151
BseBI CCWGG 4 cut(s) 743, 1186, 1355, 1611
BseCI ATCGAT 1 cut(s) 462
BseDI CCNNGG 3 cut(s) 279, 1354, 2510
BseGI GGATG 6 cut(s) 16, 143, 160, 345, 939, 2821
BseJI GATNNNNATC 4 cut(s) 468, 1161, 2223, 2811
BseLI CCNNNNNNNGG 9 cut(s) 125, 359, 624, 1185, 1538, 1616, 2161, 2516, 2838
BseMI GCAATG 2 cut(s) 2343, 2700
BseMII CTCAG 2 cut(s) 1103, 1212
BseNI ACTGG 8 cut(s) 104, 556, 844, 901, 1147, 1689, 2715, 2832
BseRI GAGGAG 3 cut(s) 1554, 1680, 2425
BseSI GKGCMC 2 cut(s) 365, 2467
BseXI GCAGC 4 cut(s) 135, 784, 1411, 1635
BseYI CCCAGC 2 cut(s) 1508, 1605
BsgI GTGCAG 1 cut(s) 1662
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 8 cut(s) 159, 700, 1096, 1866, 2076, 2107, 2258, 2599
BshNI GGYRCC 3 cut(s) 341, 362, 2323
BshVI ATCGAT 1 cut(s) 462
BsiEI CGRYCG 1 cut(s) 216
BsiHKAI GWGCWC 1 cut(s) 2068
BsiSI CCGG 5 cut(s) 152, 711, 912, 1092, 2296
BslFI GGGAC 4 cut(s) 86, 282, 681, 1531
BslI CCNNNNNNNGG 9 cut(s) 125, 359, 624, 1185, 1538, 1616, 2161, 2516, 2838
BsmAI GTCTC 4 cut(s) 1283, 1536, 2184, 2746
BsmFI GGGAC 4 cut(s) 86, 282, 681, 1531
BsmI GAATGC 2 cut(s) 531, 1240
BsnI GGCC 8 cut(s) 159, 700, 1096, 1866, 2076, 2107, 2258, 2599
Bsp119I TTCGAA 1 cut(s) 2721
Bsp1286I GDGCHC 3 cut(s) 365, 2068, 2467
Bsp13I TCCGGA 1 cut(s) 151
Bsp1407I TGTACA 1 cut(s) 1227
Bsp143I GATC 9 cut(s) 115, 217, 288, 1491, 2178, 2506, 2655, 2662, 2806
BspACI CCGC 9 cut(s) 126, 183, 325, 494, 751, 1626, 1661, 1884, 2428
BspANI GGCC 8 cut(s) 159, 700, 1096, 1866, 2076, 2107, 2258, 2599
BspCNI CTCAG 2 cut(s) 1104, 1213
BspDI ATCGAT 1 cut(s) 462
BspEI TCCGGA 1 cut(s) 151
BspHI TCATGA 1 cut(s) 2803
BspLI GGNNCC 4 cut(s) 343, 364, 2325, 2508
BspMAI CTGCAG 1 cut(s) 1429
BspMI ACCTGC 4 cut(s) 188, 1418, 1628, 1770
BspPI GGATC 5 cut(s) 110, 1486, 2501, 2514, 2657
BspT104I TTCGAA 1 cut(s) 2721
BspT107I GGYRCC 3 cut(s) 341, 362, 2323
BsrBI CCGCTC 1 cut(s) 2430
BsrDI GCAATG 2 cut(s) 2343, 2700
BsrGI TGTACA 1 cut(s) 1227
BsrI ACTGG 8 cut(s) 104, 556, 844, 901, 1147, 1689, 2715, 2832
BssECI CCNNGG 3 cut(s) 279, 1354, 2510
BssMI GATC 9 cut(s) 115, 217, 288, 1491, 2178, 2506, 2655, 2662, 2806
BssSI CACGAG 1 cut(s) 2061
BssT1I CCWWGG 2 cut(s) 279, 2510
Bst2BI CACGAG 1 cut(s) 2061
Bst2UI CCWGG 4 cut(s) 743, 1186, 1355, 1611
Bst4CI ACNGT 7 cut(s) 191, 401, 614, 919, 1300, 2526, 2775
Bst6I CTCTTC 1 cut(s) 1005
BstAPI GCANNNNNTGC 1 cut(s) 1424
BstAUI TGTACA 1 cut(s) 1227
BstBI TTCGAA 1 cut(s) 2721
BstC8I GCNNGC 1 cut(s) 924
BstDEI CTNAG 3 cut(s) 821, 1112, 1221
BstEII GGTNACC 1 cut(s) 2735
BstENI CCTNNNNNAGG 2 cut(s) 1536, 2159
BstF5I GGATG 6 cut(s) 16, 143, 160, 345, 939, 2821
BstKTI GATC 9 cut(s) 118, 220, 291, 1494, 2181, 2509, 2658, 2665, 2809
BstMAI GTCTC 4 cut(s) 1283, 1536, 2184, 2746
BstMBI GATC 9 cut(s) 115, 217, 288, 1491, 2178, 2506, 2655, 2662, 2806
BstMCI CGRYCG 1 cut(s) 216
BstMWI GCNNNNNNNGC 3 cut(s) 1143, 1424, 2113
BstNI CCWGG 4 cut(s) 743, 1186, 1355, 1611
BstNSI RCATGY 2 cut(s) 799, 907
BstPAI GACNNNNGTC 1 cut(s) 1548
BstPI GGTNACC 1 cut(s) 2735
BstSCI CCNGG 6 cut(s) 741, 1091, 1184, 1353, 1609, 2294
BstSFI CTRYAG 2 cut(s) 190, 1425
BstSLI GKGCMC 2 cut(s) 365, 2467
BstV1I GCAGC 4 cut(s) 135, 784, 1411, 1635
BstV2I GAAGAC 4 cut(s) 1366, 1663, 2577, 2601
BstX2I RGATCY 3 cut(s) 115, 1491, 2506
BstXI CCANNNNNNTGG 1 cut(s) 930
BstYI RGATCY 3 cut(s) 115, 1491, 2506
Bsu15I ATCGAT 1 cut(s) 462
BsuRI GGCC 8 cut(s) 159, 700, 1096, 1866, 2076, 2107, 2258, 2599
BsuTUI ATCGAT 1 cut(s) 462
BtrI CACGTC 1 cut(s) 1364
BtsCI GGATG 6 cut(s) 16, 143, 160, 345, 939, 2821
BtsI GCAGTG 2 cut(s) 1461, 1650
BtsIMutI CAGTG 4 cut(s) 1461, 1650, 2410, 2619
BveI ACCTGC 4 cut(s) 188, 1418, 1628, 1770
Cac8I GCNNGC 1 cut(s) 924
CaiI CAGNNNCTG 2 cut(s) 262, 1424
CciI TCATGA 1 cut(s) 2803
Cfr13I GGNCC 9 cut(s) 698, 1094, 1550, 1721, 1759, 1850, 1864, 2075, 2497
ClaI ATCGAT 1 cut(s) 462
CsiI ACCWGGT 1 cut(s) 741
Csp6I GTAC 7 cut(s) 59, 144, 994, 1028, 1228, 1482, 2038
CspCI CAANNNNNGTGG 4 cut(s) 748, 783, 2541, 2576
CviQI GTAC 7 cut(s) 59, 144, 994, 1028, 1228, 1482, 2038
DdeI CTNAG 3 cut(s) 821, 1112, 1221
DpnI GATC 9 cut(s) 117, 219, 290, 1493, 2180, 2508, 2657, 2664, 2808
DpnII GATC 9 cut(s) 115, 217, 288, 1491, 2178, 2506, 2655, 2662, 2806
DraI TTTAAA 1 cut(s) 807
EaeI YGGCCR 3 cut(s) 157, 2105, 2256
Eam1104I CTCTTC 1 cut(s) 1005
EarI CTCTTC 1 cut(s) 1005
Eco130I CCWWGG 2 cut(s) 279, 2510
Eco147I AGGCCT 1 cut(s) 2599
Eco32I GATATC 1 cut(s) 847
Eco47I GGWCC 5 cut(s) 1550, 1721, 1759, 1850, 2497
Eco57I CTGAAG 2 cut(s) 2454, 2595
Eco91I GGTNACC 1 cut(s) 2735
EcoNI CCTNNNNNAGG 2 cut(s) 1536, 2159
EcoO109I RGGNCCY 1 cut(s) 1864
EcoO65I GGTNACC 1 cut(s) 2735
EcoRI GAATTC 1 cut(s) 1695
EcoRII CCWGG 4 cut(s) 741, 1184, 1353, 1609
EcoRV GATATC 1 cut(s) 847
EcoT14I CCWWGG 2 cut(s) 279, 2510
ErhI CCWWGG 2 cut(s) 279, 2510
FaqI GGGAC 4 cut(s) 86, 282, 681, 1531
FauI CCCGC 2 cut(s) 332, 1654
FbaI TGATCA 1 cut(s) 288
FblI GTMKAC 1 cut(s) 800
Fnu4HI GCNGC 5 cut(s) 124, 184, 773, 1425, 1624
FokI GGATG 6 cut(s) 23, 150, 167, 332, 946, 2808
Fsp4HI GCNGC 5 cut(s) 124, 184, 773, 1425, 1624
GluI GCNGC 5 cut(s) 124, 184, 773, 1425, 1624
GsaI CCCAGC 2 cut(s) 1512, 1609
GsuI CTGGAG 3 cut(s) 336, 501, 861
HaeIII GGCC 8 cut(s) 159, 700, 1096, 1866, 2076, 2107, 2258, 2599
HapII CCGG 5 cut(s) 152, 711, 912, 1092, 2296
HincII GTYRAC 1 cut(s) 801
HindII GTYRAC 1 cut(s) 801
HindIII AAGCTT 5 cut(s) 63, 578, 1901, 1988, 2261
HpaII CCGG 5 cut(s) 152, 711, 912, 1092, 2296
HphI GGTGA 6 cut(s) 313, 430, 2104, 2629, 2665, 2747
Hpy166II GTNNAC 9 cut(s) 801, 965, 996, 1108, 1303, 1461, 1759, 1858, 2781
Hpy8I GTNNAC 9 cut(s) 801, 965, 996, 1108, 1303, 1461, 1759, 1858, 2781
Hpy99I CGWCG 1 cut(s) 2779
HpyAV CCTTC 9 cut(s) 377, 717, 1107, 1744, 1832, 1877, 2003, 2610, 2624
HpyCH4III ACNGT 7 cut(s) 191, 401, 614, 919, 1300, 2526, 2775
HpyCH4IV ACGT 3 cut(s) 174, 1363, 1470
HpyF10VI GCNNNNNNNGC 3 cut(s) 1143, 1424, 2113
HpyF3I CTNAG 3 cut(s) 821, 1112, 1221
HpySE526I ACGT 3 cut(s) 174, 1363, 1470
Kpn2I TCCGGA 1 cut(s) 151
Ksp22I TGATCA 1 cut(s) 288
Kzo9I GATC 9 cut(s) 115, 217, 288, 1491, 2178, 2506, 2655, 2662, 2806
LmnI GCTCC 2 cut(s) 154, 496
Lsp1109I GCAGC 4 cut(s) 135, 784, 1411, 1635
LweI GCATC 2 cut(s) 1139, 1411
MabI ACCWGGT 1 cut(s) 741
MaeII ACGT 3 cut(s) 174, 1363, 1470
MaeIII GTNAC 8 cut(s) 175, 232, 608, 1389, 1447, 1471, 2414, 2735
MalI GATC 9 cut(s) 117, 219, 290, 1493, 2180, 2508, 2657, 2664, 2808
MbiI CCGCTC 1 cut(s) 2430
MboI GATC 9 cut(s) 115, 217, 288, 1491, 2178, 2506, 2655, 2662, 2806
MfeI CAATTG 2 cut(s) 1887, 2766
MflI RGATCY 3 cut(s) 115, 1491, 2506
MhlI GDGCHC 3 cut(s) 365, 2068, 2467
MlsI TGGCCA 3 cut(s) 159, 2107, 2258
MluNI TGGCCA 3 cut(s) 159, 2107, 2258
MlyI GAGTC 3 cut(s) 1197, 1677, 2755
MmeI TCCRAC 4 cut(s) 373, 1630, 2373, 2475
Mox20I TGGCCA 3 cut(s) 159, 2107, 2258
MroI TCCGGA 1 cut(s) 151
MroXI GAANNNNTTC 1 cut(s) 1712
MscI TGGCCA 3 cut(s) 159, 2107, 2258
Msp20I TGGCCA 3 cut(s) 159, 2107, 2258
MspA1I CMGCKG 3 cut(s) 126, 926, 1626
MspI CCGG 5 cut(s) 152, 711, 912, 1092, 2296
MspR9I CCNGG 6 cut(s) 743, 1093, 1186, 1355, 1611, 2296
MunI CAATTG 2 cut(s) 1887, 2766
Mva1269I GAATGC 2 cut(s) 531, 1240
MvaI CCWGG 4 cut(s) 743, 1186, 1355, 1611
MwoI GCNNNNNNNGC 3 cut(s) 1143, 1424, 2113
NciI CCSGG 2 cut(s) 1093, 2296
NdeII GATC 9 cut(s) 115, 217, 288, 1491, 2178, 2506, 2655, 2662, 2806
NlaIV GGNNCC 4 cut(s) 343, 364, 2325, 2508
NmuCI GTSAC 5 cut(s) 232, 608, 1389, 1447, 2735
NspI RCATGY 2 cut(s) 799, 907
NspV TTCGAA 1 cut(s) 2721
PagI TCATGA 1 cut(s) 2803
PaqCI CACCTGC 2 cut(s) 1418, 1628
PceI AGGCCT 1 cut(s) 2599
PciI ACATGT 1 cut(s) 795
PcsI WCGNNNNNNNCGW 1 cut(s) 1068
PctI GAATGC 2 cut(s) 531, 1240
PdmI GAANNNNTTC 1 cut(s) 1712
PfeI GAWTC 8 cut(s) 259, 676, 1216, 1577, 1653, 1927, 2165, 2828
PflMI CCANNNNNTGG 2 cut(s) 1616, 2838
PkrI GCNGC 5 cut(s) 125, 185, 774, 1426, 1625
PleI GAGTC 3 cut(s) 1197, 1676, 2754
PpsI GAGTC 3 cut(s) 1197, 1676, 2754
PscI ACATGT 1 cut(s) 795
PshAI GACNNNNGTC 1 cut(s) 1548
Psp1406I AACGTT 1 cut(s) 174
Psp6I CCWGG 4 cut(s) 741, 1184, 1353, 1609
PspEI GGTNACC 1 cut(s) 2735
PspFI CCCAGC 2 cut(s) 1508, 1605
PspGI CCWGG 4 cut(s) 741, 1184, 1353, 1609
PspN4I GGNNCC 4 cut(s) 343, 364, 2325, 2508
PspPI GGNCC 9 cut(s) 698, 1094, 1550, 1721, 1759, 1850, 1864, 2075, 2497
PstI CTGCAG 1 cut(s) 1429
PstNI CAGNNNCTG 2 cut(s) 262, 1424
PsuI RGATCY 3 cut(s) 115, 1491, 2506
PvuII CAGCTG 1 cut(s) 926
RsaI GTAC 7 cut(s) 60, 145, 995, 1029, 1229, 1483, 2039
RsaNI GTAC 7 cut(s) 59, 144, 994, 1028, 1228, 1482, 2038
SalI GTCGAC 1 cut(s) 799
SatI GCNGC 5 cut(s) 124, 184, 773, 1425, 1624
Sau3AI GATC 9 cut(s) 115, 217, 288, 1491, 2178, 2506, 2655, 2662, 2806
Sau96I GGNCC 9 cut(s) 698, 1094, 1550, 1721, 1759, 1850, 1864, 2075, 2497
ScaI AGTACT 2 cut(s) 145, 2039
SchI GAGTC 3 cut(s) 1197, 1677, 2755
ScrFI CCNGG 6 cut(s) 743, 1093, 1186, 1355, 1611, 2296
SduI GDGCHC 3 cut(s) 365, 2068, 2467
SexAI ACCWGGT 1 cut(s) 741
SfaNI GCATC 2 cut(s) 1139, 1411
SfcI CTRYAG 2 cut(s) 190, 1425
SfuI TTCGAA 1 cut(s) 2721
SinI GGWCC 5 cut(s) 1550, 1721, 1759, 1850, 2497
SmlI CTYRAG 4 cut(s) 662, 1596, 1783, 2741
SmoI CTYRAG 4 cut(s) 662, 1596, 1783, 2741
SpeI ACTAGT 2 cut(s) 373, 670
SseBI AGGCCT 1 cut(s) 2599
SsiI CCGC 9 cut(s) 126, 183, 325, 494, 751, 1626, 1661, 1884, 2428
SspI AATATT 3 cut(s) 763, 1326, 1414
StuI AGGCCT 1 cut(s) 2599
StyD4I CCNGG 6 cut(s) 741, 1091, 1184, 1353, 1609, 2294
StyI CCWWGG 2 cut(s) 279, 2510
TaaI ACNGT 7 cut(s) 191, 401, 614, 919, 1300, 2526, 2775
TaiI ACGT 3 cut(s) 177, 1366, 1473
TaqI TCGA 6 cut(s) 462, 539, 800, 1371, 2217, 2721
TaqII GACCGA 1 cut(s) 230
TatI WGTACW 4 cut(s) 58, 143, 1227, 2037
TauI GCSGC 1 cut(s) 186
TfiI GAWTC 8 cut(s) 259, 676, 1216, 1577, 1653, 1927, 2165, 2828
TscAI CASTG 4 cut(s) 1468, 1650, 2410, 2619
TseFI GTSAC 5 cut(s) 232, 608, 1389, 1447, 2735
TseI GCWGC 4 cut(s) 123, 772, 1424, 1623
Tsp45I GTSAC 5 cut(s) 232, 608, 1389, 1447, 2735
TspGWI ACGGA 1 cut(s) 1484
TspRI CASTG 4 cut(s) 1468, 1650, 2410, 2619
Van91I CCANNNNNTGG 2 cut(s) 1616, 2838
VpaK11BI GGWCC 5 cut(s) 1550, 1721, 1759, 1850, 2497
XagI CCTNNNNNAGG 2 cut(s) 1536, 2159
XapI RAATTY 6 cut(s) 103, 505, 810, 1042, 1695, 1934
XceI RCATGY 2 cut(s) 799, 907
XcmI CCANNNNNNNNNTGG 2 cut(s) 1613, 2611
XmiI GTMKAC 1 cut(s) 800
XmnI GAANNNNTTC 1 cut(s) 1712
ZrmI AGTACT 2 cut(s) 145, 2039
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.