Rroxscaffold_1G00050120

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000292
Physical Location & Seq
Forward (+)
70481774 .. 70491227
9454 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00050120.1

Sequence Viewer

Length: 2823 bp
ATGACGCCCAGGCGAAGTCGGCACTATCCATGGCCGCAGCCGTACAAGGAACCGTTTGGTGGTGTTTTCCTCTCTCGCGACGCTAGGGCTAGAGAGAGTTACCTGACCTACATTAAGTATTTACCTACTATTATTCATGAATATTTCATCTGGGATGAAGCGCAGACTTTTGAAAGGGCAGAATCTGACAGGACAACTCCCCAAGGAGTTGATCAACCTCCCTATCTCCAAGAAATTGACCTCACCCGCAACTACCTGAATGGCACCATCCCTCCAGAATGGGGCTCCCTGCCACTAGTTAGCATGTACACTCTTATCTCTATCTGCATATATATGTCTGCATTGGCTTCTCTTGTTGGAAACCGTTTGACAGGTTCTATCCCTGTTGAGCTAGGAAACATCACCACGCTCAAAAGTTTGACCATCGATTTCAATCGTTTATCTGGAGTTCTTCCTGCGGAGCTTGGAAATTTGTCTAGCATAGAAAGAATGCTTCTTACCTCGAACAATTTTACTGGGGAGTTGCCAGAAACATTTGCAAAGCTTACCACCTTAACTGACTTTCGGATTAGTGACAGTTCCTTTTCTGGAAAGATACCTGATTTTATTCAGAGCTGGACAAATCTTGAGGGACTAGTGATTCAGGCTAGTGGTTTTACAGGGCCAATTCCTTCCGGCATTTCTCTCTTGACAAGATTAACTGACCTGAGAATTACTGACTTGAATGGATCTGAATCATCCTTTCCTGTACTTGATAATATGAAAAGCCTGGAGACATTGGTGTTGCGAAACTGCAATATTAGTGGGCAGCTACCTGAATATCTTGGGAACATGTCGACTTTAAAAATTTTAGACCTTAGTTTTAACAAGCTAACTGGAGATATCCCAAACAACTTTGCTGGTCTAGGAAATCTGGATAATATGTTCTTAACTGGAAACATGCTGACCGGAACTGTGCCAGCTGATTGGATGAAAGCCAATAGCATTGATCTTTCATATAACAAATTCACCATCAACTCAAATGATGAGAATTGTGAACCACGAGATGGCTTGAACTTGTTTGCAAGCTCTTCAGGTGGCAATACTTCATTTGTTTCATGTTTACCACTTGCAAAATGTGTAAAAACTTGGTACACTCTTCATATAAATTGTGGTGGAAAAGAAGTACCCCTCTATGAAAATTTCACAAGACCCACCAATGCAACATACGAGGAAGATACTGATGTAGCCGGGCCTTCATCAGTTTACCTGAGCAGGACAAATTGGGCATCAAGCACGACTGGCTACTTCGCTGATGATAATCGTCTTACTAAAACCTATACCTGGACTAATTCATCTGGACTCAATATGGCGAATCCTGAGTTGTACATGAATGCACGCCTTTCTCCTATTTCTCTAACTTACTATGGGTTTTGTCTTGGAAATGGAAGCTACACAGGGTCACTAGCGTTCAAGGATTTCAATATTGCAGATGCTGCAGGTGGCTTTGGAAACGCAGTCACAAAAAAGTTCACTGCTAACGTAACTGCTGGTACTTTGGAGATCCGTTTATTCTGGGCTGGGAAAGGGACAACAGGTGTTCCTTACAGAGGAGACTATGGTCCTCTTATATCAGCTATCTCTATAGATTCTGACTTTCCACCCCCTCAAGAAACCCAGCCAGGAGGTGGAAGCAGCGGTATATCAGCAGGTGCAGTGGTTGGAATCGTGGCGGGAGGAGTCTTCATTGTATTACTGGTTTTAGGAATTCTCTGGTGGAAAGGGTTCATAGGACCAGCAAATACTTTAGAACAAGATTTGAAGGGTGTAGACCTGCAAACCAGCAGATTTACCTTGAGGCAAATCAAAGTTGCCACAAACAACTTTGACATATCAAATAAGATTGGAGAAGGTGGTTTTGGTCCTGTTTACAAGGGCCTTCTATCAGATGACACCGCAATTGCTGTTAAGAAGCTTTCTGCCAAATCAAAGCAAGGGAATCGTGAATTTGTGAATGAGATTGGCATGATTTCTGCTCTGCAACACCCTTACCTTGTCAAGCTTTATGGATGTTGTATTGAAGGAAATGACTTGTTGCTTGTCTATGAGTACTTGGAAAATAATAGCGTTGCTCGTGCTCTGTTTGGCCCAAAAGCAAGTCAGGTGAAGATGGATTGGCCAACAAGGCACAGGATCTGTGTTGGTATAGCAAAAGGTTTGGCTTACCTCCATGAGGAATCAAGGTTGAAGATCGTTCATAGAGACATTAAAGCTACTAATGTGCTACTCGATAAAAATCTTTGTCCAAAGATAGCTGATTTTGGATTGGCCAAGCTTGATGAACAGGATAATACCCACATAAGCACCCGGATTGCTGGAACTCTAGGATATATGGCACCTGAATATGCAATGCGAGGTTATCCGACTGATAAAGCAGATGTTTATAGTTTCGGAATTGTTGTGTTGGAAATAGTCAGTGGGAGGAGTAACACAACTTACCGCTCCGAAGAAGAATGTTTTTATCTTCTTGATTGGGCACTTCTTCTAAAGGAGAGAGGGAGTTTGTTGGACCTAGTGGATCCAAGGCTAGGCTCAGAGTTTAATAAAGAACAGATGATTACTACAATCAATGTGGCTCTCCTTTGCTCTCATGTGTCTTCGGCAGTTAGGCCTTCCATGTCTTCAGTGGTGAAAATGCTTGAAGGCAGGGCTTCGGTTCAGGAGGTGATCTCTGATCCAAATGCCTCAAACAATGAAATCAATGCAATGAGGAAACATTTTCAATCCAATTTCGAAGAAAACAATGGTGACCTTGAGAGTCGAGAGACAAACTATGTCAATTGA

Protein Analysis

940

Amino Acids

103.45

Weight (kDa)

5.35

Isoelectric Point (pI)

32.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 130 - 239 1.6e-06 Leucine-rich repeat region
LRR_8 PF13855 256 - 315 1.5e-08 Leucine rich repeat
Malectin PF11721 379 - 484 4.7e-08 Malectin domain
Malectin PF11721 480 - 540 4.8e-10 Malectin domain
Pkinase PF00069 623 - 825 1.3e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 624 - 829 2.5e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000292)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53420 AT3G14840
fragaria_vesca FvH4_3g17840 FvH4_3g17840 FvH4_3g17840 FvH4_3g17840 FvH4_3g17840 FvH4_3g17840 FvH4_3g17841 FvH4_3g17841 FvH4_3g17841 FvH4_3g17841 FvH4_3g17841 FvH4_3g17841 FvH4_3g17850 FvH4_3g17850 FvH4_3g17850 FvH4_3g17850 FvH4_3g17851
malus_domestica MD03G1246600.v1.1 MD06G1045200.v1.1 MD06G1045400.v1.1 MD11G1267800.v1.1 MD11G1267900.v1.1 MD11G1268100.v1.1 MD11G1268300.v1.1 MD11G1268500.v1.1 MD11G1268600.v1.1
prunus_persica Prupe.4G157700_v2.0.a1 Prupe.4G157700_v2.0.a1 Prupe.4G157800_v2.0.a1 Prupe.4G157800_v2.0.a1 Prupe.4G157800_v2.0.a1 Prupe.4G157800_v2.0.a1 Prupe.4G157800_v2.0.a1 Prupe.4G157900_v2.0.a1 Prupe.4G157900_v2.0.a1 Prupe.4G157900_v2.0.a1
pyrus_communis pycom03g19540 pycom11g23600 pycom11g23630 pycom11g23690 pycom11g23720 pycom11g23730 pycom11g23740 pycom11g23750
rosa_chinensis RchiOBHm_Chr0c40g0503411 RchiOBHm_Chr0c40g0503421 RchiOBHm_Chr5g0029631 RchiOBHm_Chr5g0029661 RchiOBHm_Chr5g0029701 RchiOBHm_Chr5g0029741 RchiOBHm_Chr5g0029771 RchiOBHm_Chr5g0029781 RchiOBHm_Chr5g0029811 RchiOBHm_Chr5g0029851 RchiOBHm_Chr5g0029871 RchiOBHm_Chr5g0029891 RchiOBHm_Chr7g0222221
rosa_laevigata RLG00000017380 RLG00000032873 RLG00000033188 RLG00000033190 RLG00000033192 RLG00000033193 RLG00000033194 RLG00000033196 RLG00000033199 RLG00000033238
rosa_multiflora Rmu_co8359987.1_g000001 Rmu_co8444909.1_g000001 Rmu_sc0001608.1_g000007 Rmu_sc0001608.1_g000009 Rmu_sc0001608.1_g000013 Rmu_sc0001608.1_g000018 Rmu_sc0001608.1_g000023 Rmu_sc0001608.1_g000040 Rmu_sc0003238.1_g000016 Rmu_sc0003368.1_g000026 Rmu_sc0003368.1_g000034 Rmu_sc0003368.1_g000043 Rmu_sc0003368.1_g000047 Rmu_sc0003368.1_g000052 Rmu_sc0003368.1_g000054 Rmu_sc0004963.1_g000001
rosa_roxburghii Rroxscaffold_1G00050080 Rroxscaffold_1G00050090 Rroxscaffold_1G00050110 Rroxscaffold_1G00050120 Rroxscaffold_1G00050180 Rroxscaffold_1G00050190 Rroxscaffold_1G00050220 Rroxscaffold_1G00050270 Rroxscaffold_1G00050300
rosa_rugosa Rorug04G0065200 Rorug05G0114900 Rorug05G0115000 Rorug05G0115100 Rorug05G0120100 Rorug05G0238900
rosa_samantha Rh5AG207800 Rh5AG208000 Rh5AG208300 Rh5AG208400 Rh5AG208800 Rh5AG294900 Rh5AG295000 Rh5AG295400 Rh5CG230400 Rh7AG347800
rosa_wichuraiana Rw0G005370 Rw0G012460 Rw0G019060 Rw5G018910 Rw5G018920 Rw5G018930 Rw5G018940 Rw5G018960 Rw5G018970 Rw5G018980 Rw5G018990 Rw5G019000 Rw7G029840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1469, 1679
Acc36I ACCTGC 3 cut(s) 1469, 1679, 1821
AccB1I GGYRCC 2 cut(s) 263, 2374
AccB7I CCANNNNNTGG 2 cut(s) 1046, 1667
AccBSI CCGCTC 1 cut(s) 2481
AccI GTMKAC 2 cut(s) 836, 1809
AccII CGCG 1 cut(s) 78
AciI CCGC 7 cut(s) 35, 247, 458, 1677, 1712, 1935, 2479
AclWI GGATC 6 cut(s) 736, 1537, 2180, 2552, 2565, 2708
AcoI YGGCCR 3 cut(s) 32, 2156, 2307
AcsI RAATTY 6 cut(s) 469, 846, 1004, 1180, 1746, 1985
AcuI CTGAAG 2 cut(s) 1056, 2646
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 8 cut(s) 44, 308, 750, 1133, 1167, 1367, 1534, 2090
AfiI CCNNNNNNNGG 8 cut(s) 59, 281, 1046, 1323, 1589, 1667, 2212, 2567
AflIII ACRYGT 1 cut(s) 831
AhlI ACTAGT 2 cut(s) 295, 634
AjnI CCWGG 4 cut(s) 8, 768, 1322, 1660
AjuI GAANNNNNNNTTGG 2 cut(s) 1881, 1913
AloI GAACNNNNNNTCC 2 cut(s) 908, 940
Alw21I GWGCWC 1 cut(s) 2119
Alw26I GTCTC 4 cut(s) 767, 1587, 2235, 2798
AlwI GGATC 6 cut(s) 736, 1537, 2180, 2552, 2565, 2708
AlwNI CAGNNNCTG 3 cut(s) 185, 1475, 2175
AoxI GGCC 8 cut(s) 32, 662, 1232, 1915, 2125, 2156, 2307, 2648
ApeKI GCWGC 4 cut(s) 37, 808, 1475, 1674
ApoI RAATTY 6 cut(s) 469, 846, 1004, 1180, 1746, 1985
Asp700I GAANNNNTTC 1 cut(s) 1763
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 8 cut(s) 662, 1232, 1601, 1772, 1901, 1915, 2126, 2548
AsuC2I CCSGG 2 cut(s) 1231, 2347
AsuHPI GGTGA 7 cut(s) 235, 394, 1000, 2155, 2680, 2716, 2798
AsuII TTCGAA 1 cut(s) 2772
AvaII GGWCC 4 cut(s) 1601, 1772, 1901, 2548
BaeGI GKGCMC 1 cut(s) 2518
BaeI ACNNNNGTAYC 2 cut(s) 1209, 1242
BalI TGGCCA 2 cut(s) 2158, 2309
BamHI GGATCC 1 cut(s) 2557
BanI GGYRCC 2 cut(s) 263, 2374
BanII GRGCYC 1 cut(s) 287
BauI CACGAG 2 cut(s) 1041, 2112
BbsI GAAGAC 3 cut(s) 1714, 2628, 2652
Bbv12I GWGCWC 1 cut(s) 2119
BbvI GCAGC 4 cut(s) 49, 820, 1462, 1686
BccI CCATC 5 cut(s) 275, 431, 1019, 1040, 2143
BceAI ACGGC 1 cut(s) 25
BcgI CGANNNNNNTGC 2 cut(s) 1961, 1995
BciT130I CCWGG 4 cut(s) 10, 770, 1324, 1662
BclI TGATCA 1 cut(s) 211
BcnI CCSGG 2 cut(s) 1231, 2347
BcoDI GTCTC 4 cut(s) 767, 1587, 2235, 2798
BcuI ACTAGT 2 cut(s) 295, 634
BfmI CTRYAG 2 cut(s) 1476, 1623
BfuAI ACCTGC 3 cut(s) 1469, 1679, 1821
BglI GCCNNNNNGGC 1 cut(s) 2164
BisI GCNGC 5 cut(s) 35, 38, 809, 1476, 1675
BlsI GCNGC 5 cut(s) 36, 39, 810, 1477, 1676
BmcAI AGTACT 1 cut(s) 2090
Bme1390I CCNGG 6 cut(s) 10, 770, 1231, 1324, 1662, 2347
Bme18I GGWCC 4 cut(s) 1601, 1772, 1901, 2548
BmgT120I GGNCC 8 cut(s) 662, 1232, 1601, 1772, 1901, 1915, 2126, 2548
BmiI GGNNCC 5 cut(s) 51, 265, 286, 2376, 2559
BmrFI CCNGG 6 cut(s) 10, 770, 1231, 1324, 1662, 2347
BmrI ACTGGG 1 cut(s) 525
BmsI GCATC 2 cut(s) 1277, 1462
BmuI ACTGGG 1 cut(s) 525
BoxI GACNNNNGTC 1 cut(s) 1599
BpiI GAAGAC 3 cut(s) 1714, 2628, 2652
BplI GAGNNNNNCTC 2 cut(s) 2693, 2725
BpmI CTGGAG 4 cut(s) 258, 465, 791, 897
Bpu10I CCTNAGC 1 cut(s) 1250
Bpu14I TTCGAA 1 cut(s) 2772
BpuEI CTTGAG 4 cut(s) 647, 1632, 1855, 2813
BpuMI CCSGG 2 cut(s) 1231, 2347
Bsa29I ATCGAT 1 cut(s) 426
BsaBI GATNNNNATC 4 cut(s) 432, 733, 1299, 2274
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 4 cut(s) 8, 29, 202, 2561
BsaWI WCCGGW 1 cut(s) 947
BsaXI ACNNNNNCTCC 2 cut(s) 256, 286
Bsc4I CCNNNNNNNGG 8 cut(s) 59, 281, 1046, 1323, 1589, 1667, 2212, 2567
Bse1I ACTGG 5 cut(s) 520, 880, 937, 1285, 1740
Bse3DI GCAATG 2 cut(s) 2394, 2751
Bse8I GATNNNNATC 4 cut(s) 432, 733, 1299, 2274
BseBI CCWGG 4 cut(s) 10, 770, 1324, 1662
BseCI ATCGAT 1 cut(s) 426
BseDI CCNNGG 4 cut(s) 8, 29, 202, 2561
BseGI GGATG 5 cut(s) 160, 267, 737, 975, 2054
BseJI GATNNNNATC 4 cut(s) 432, 733, 1299, 2274
BseLI CCNNNNNNNGG 8 cut(s) 59, 281, 1046, 1323, 1589, 1667, 2212, 2567
BseMI GCAATG 2 cut(s) 2394, 2751
BseMII CTCAG 4 cut(s) 698, 1241, 1350, 2586
BseNI ACTGG 5 cut(s) 520, 880, 937, 1285, 1740
BseRI GAGGAG 3 cut(s) 1605, 1731, 2476
BseSI GKGCMC 1 cut(s) 2518
BseXI GCAGC 4 cut(s) 49, 820, 1462, 1686
BseYI CCCAGC 2 cut(s) 1559, 1656
BsgI GTGCAG 1 cut(s) 1713
Bsh1236I CGCG 1 cut(s) 78
BshFI GGCC 8 cut(s) 34, 664, 1234, 1917, 2127, 2158, 2309, 2650
BshNI GGYRCC 2 cut(s) 263, 2374
BshVI ATCGAT 1 cut(s) 426
BsiHKAI GWGCWC 1 cut(s) 2119
BsiSI CCGG 4 cut(s) 675, 948, 1230, 2347
BslFI GGGAC 2 cut(s) 645, 1582
BslI CCNNNNNNNGG 8 cut(s) 59, 281, 1046, 1323, 1589, 1667, 2212, 2567
BsmAI GTCTC 4 cut(s) 767, 1587, 2235, 2798
BsmFI GGGAC 2 cut(s) 645, 1582
BsmI GAATGC 2 cut(s) 495, 1378
BsnI GGCC 8 cut(s) 34, 664, 1234, 1917, 2127, 2158, 2309, 2650
Bsp119I TTCGAA 1 cut(s) 2772
Bsp1286I GDGCHC 3 cut(s) 287, 2119, 2518
Bsp1407I TGTACA 2 cut(s) 306, 1365
Bsp143I GATC 9 cut(s) 211, 728, 988, 1542, 2172, 2229, 2557, 2706, 2713
Bsp19I CCATGG 1 cut(s) 29
Bsp68I TCGCGA 1 cut(s) 78
BspACI CCGC 7 cut(s) 35, 247, 458, 1677, 1712, 1935, 2479
BspANI GGCC 8 cut(s) 34, 664, 1234, 1917, 2127, 2158, 2309, 2650
BspCNI CTCAG 4 cut(s) 699, 1242, 1351, 2585
BspDI ATCGAT 1 cut(s) 426
BspFNI CGCG 1 cut(s) 78
BspHI TCATGA 1 cut(s) 136
BspLI GGNNCC 5 cut(s) 51, 265, 286, 2376, 2559
BspMAI CTGCAG 1 cut(s) 1480
BspMI ACCTGC 3 cut(s) 1469, 1679, 1821
BspPI GGATC 6 cut(s) 736, 1537, 2180, 2552, 2565, 2708
BspQI GCTCTTC 1 cut(s) 1075
BspT104I TTCGAA 1 cut(s) 2772
BspT107I GGYRCC 2 cut(s) 263, 2374
BsrBI CCGCTC 1 cut(s) 2481
BsrDI GCAATG 2 cut(s) 2394, 2751
BsrGI TGTACA 2 cut(s) 306, 1365
BsrI ACTGG 5 cut(s) 520, 880, 937, 1285, 1740
BssECI CCNNGG 4 cut(s) 8, 29, 202, 2561
BssMI GATC 9 cut(s) 211, 728, 988, 1542, 2172, 2229, 2557, 2706, 2713
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 2 cut(s) 1041, 2112
BssT1I CCWWGG 3 cut(s) 29, 202, 2561
Bst2BI CACGAG 2 cut(s) 1041, 2112
Bst2UI CCWGG 4 cut(s) 10, 770, 1324, 1662
Bst4CI ACNGT 4 cut(s) 54, 365, 578, 955
Bst6I CTCTTC 2 cut(s) 1075, 1143
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 1475
BstAUI TGTACA 2 cut(s) 306, 1365
BstBI TTCGAA 1 cut(s) 2772
BstC8I GCNNGC 3 cut(s) 960, 1066, 1378
BstDEI CTNAG 5 cut(s) 707, 857, 1250, 1359, 2572
BstDSI CCRYGG 1 cut(s) 29
BstEII GGTNACC 1 cut(s) 2786
BstENI CCTNNNNNAGG 2 cut(s) 1587, 2210
BstF5I GGATG 5 cut(s) 160, 267, 737, 975, 2054
BstFNI CGCG 1 cut(s) 78
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 9 cut(s) 214, 731, 991, 1545, 2175, 2232, 2560, 2709, 2716
BstMAI GTCTC 4 cut(s) 767, 1587, 2235, 2798
BstMBI GATC 9 cut(s) 211, 728, 988, 1542, 2172, 2229, 2557, 2706, 2713
BstMWI GCNNNNNNNGC 4 cut(s) 19, 1281, 1475, 2164
BstNI CCWGG 4 cut(s) 10, 770, 1324, 1662
BstNSI RCATGY 3 cut(s) 307, 835, 943
BstPAI GACNNNNGTC 1 cut(s) 1599
BstPI GGTNACC 1 cut(s) 2786
BstSCI CCNGG 6 cut(s) 8, 768, 1229, 1322, 1660, 2345
BstSFI CTRYAG 2 cut(s) 1476, 1623
BstSLI GKGCMC 1 cut(s) 2518
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 4 cut(s) 49, 820, 1462, 1686
BstV2I GAAGAC 3 cut(s) 1714, 2628, 2652
BstX2I RGATCY 4 cut(s) 728, 1542, 2172, 2557
BstXI CCANNNNNNTGG 1 cut(s) 966
BstYI RGATCY 4 cut(s) 728, 1542, 2172, 2557
Bsu15I ATCGAT 1 cut(s) 426
BsuRI GGCC 8 cut(s) 34, 664, 1234, 1917, 2127, 2158, 2309, 2650
BsuTUI ATCGAT 1 cut(s) 426
BtgI CCRYGG 1 cut(s) 29
BtsCI GGATG 5 cut(s) 160, 267, 737, 975, 2054
BtsI GCAGTG 2 cut(s) 1512, 1701
BtsIMutI CAGTG 4 cut(s) 1512, 1701, 2461, 2670
BtuMI TCGCGA 1 cut(s) 78
BveI ACCTGC 3 cut(s) 1469, 1679, 1821
Cac8I GCNNGC 3 cut(s) 960, 1066, 1378
CaiI CAGNNNCTG 3 cut(s) 185, 1475, 2175
CciI TCATGA 1 cut(s) 136
CfoI GCGC 1 cut(s) 163
Cfr13I GGNCC 8 cut(s) 662, 1232, 1601, 1772, 1901, 1915, 2126, 2548
ClaI ATCGAT 1 cut(s) 426
CseI GACGC 2 cut(s) 13, 89
Csp6I GTAC 8 cut(s) 43, 307, 749, 1132, 1166, 1366, 1533, 2089
CspCI CAANNNNNGTGG 4 cut(s) 784, 819, 2592, 2627
CviQI GTAC 8 cut(s) 43, 307, 749, 1132, 1166, 1366, 1533, 2089
DdeI CTNAG 5 cut(s) 707, 857, 1250, 1359, 2572
DpnI GATC 9 cut(s) 213, 730, 990, 1544, 2174, 2231, 2559, 2708, 2715
DpnII GATC 9 cut(s) 211, 728, 988, 1542, 2172, 2229, 2557, 2706, 2713
DraI TTTAAA 1 cut(s) 843
EaeI YGGCCR 3 cut(s) 32, 2156, 2307
Eam1104I CTCTTC 2 cut(s) 1075, 1143
EarI CTCTTC 2 cut(s) 1075, 1143
Eco130I CCWWGG 3 cut(s) 29, 202, 2561
Eco147I AGGCCT 1 cut(s) 2650
Eco24I GRGCYC 1 cut(s) 287
Eco32I GATATC 1 cut(s) 883
Eco47I GGWCC 4 cut(s) 1601, 1772, 1901, 2548
Eco57I CTGAAG 2 cut(s) 1056, 2646
Eco91I GGTNACC 1 cut(s) 2786
EcoNI CCTNNNNNAGG 2 cut(s) 1587, 2210
EcoO109I RGGNCCY 1 cut(s) 1915
EcoO65I GGTNACC 1 cut(s) 2786
EcoRI GAATTC 1 cut(s) 1746
EcoRII CCWGG 4 cut(s) 8, 768, 1322, 1660
EcoRV GATATC 1 cut(s) 883
EcoT14I CCWWGG 3 cut(s) 29, 202, 2561
EcoT38I GRGCYC 1 cut(s) 287
ErhI CCWWGG 3 cut(s) 29, 202, 2561
FaqI GGGAC 2 cut(s) 645, 1582
FauI CCCGC 2 cut(s) 254, 1705
FbaI TGATCA 1 cut(s) 211
FblI GTMKAC 2 cut(s) 836, 1809
Fnu4HI GCNGC 5 cut(s) 35, 38, 809, 1476, 1675
FokI GGATG 5 cut(s) 167, 254, 724, 982, 2061
FriOI GRGCYC 1 cut(s) 287
Fsp4HI GCNGC 5 cut(s) 35, 38, 809, 1476, 1675
GlaI GCGC 1 cut(s) 162
GluI GCNGC 5 cut(s) 35, 38, 809, 1476, 1675
GsaI CCCAGC 2 cut(s) 1563, 1660
GsuI CTGGAG 4 cut(s) 258, 465, 791, 897
HaeIII GGCC 8 cut(s) 34, 664, 1234, 1917, 2127, 2158, 2309, 2650
HapII CCGG 4 cut(s) 675, 948, 1230, 2347
HgaI GACGC 2 cut(s) 13, 89
HhaI GCGC 1 cut(s) 163
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HincII GTYRAC 1 cut(s) 837
HindII GTYRAC 1 cut(s) 837
HindIII AAGCTT 4 cut(s) 542, 1952, 2039, 2312
HpaII CCGG 4 cut(s) 675, 948, 1230, 2347
HphI GGTGA 7 cut(s) 235, 394, 1000, 2155, 2680, 2716, 2798
Hpy166II GTNNAC 9 cut(s) 309, 837, 1037, 1103, 1134, 1246, 1512, 1810, 1909
Hpy8I GTNNAC 9 cut(s) 309, 837, 1037, 1103, 1134, 1246, 1512, 1810, 1909
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 8 cut(s) 681, 1245, 1795, 1883, 1928, 2054, 2661, 2675
HpyCH4III ACNGT 4 cut(s) 54, 365, 578, 955
HpyCH4IV ACGT 1 cut(s) 1521
HpyF10VI GCNNNNNNNGC 4 cut(s) 19, 1281, 1475, 2164
HpyF3I CTNAG 5 cut(s) 707, 857, 1250, 1359, 2572
HpySE526I ACGT 1 cut(s) 1521
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 161
Ksp22I TGATCA 1 cut(s) 211
Kzo9I GATC 9 cut(s) 211, 728, 988, 1542, 2172, 2229, 2557, 2706, 2713
LguI GCTCTTC 1 cut(s) 1075
LmnI GCTCC 3 cut(s) 290, 460, 2486
Lsp1109I GCAGC 4 cut(s) 49, 820, 1462, 1686
LweI GCATC 2 cut(s) 1277, 1462
MaeII ACGT 1 cut(s) 1521
MaeIII GTNAC 7 cut(s) 98, 572, 1440, 1498, 1522, 2465, 2786
MalI GATC 9 cut(s) 213, 730, 990, 1544, 2174, 2231, 2559, 2708, 2715
MbiI CCGCTC 1 cut(s) 2481
MboI GATC 9 cut(s) 211, 728, 988, 1542, 2172, 2229, 2557, 2706, 2713
MfeI CAATTG 2 cut(s) 1938, 2818
MflI RGATCY 4 cut(s) 728, 1542, 2172, 2557
MhlI GDGCHC 3 cut(s) 287, 2119, 2518
MlsI TGGCCA 2 cut(s) 2158, 2309
MluNI TGGCCA 2 cut(s) 2158, 2309
MlyI GAGTC 3 cut(s) 1335, 1728, 2806
MmeI TCCRAC 5 cut(s) 337, 1681, 2424, 2426, 2526
Mox20I TGGCCA 2 cut(s) 2158, 2309
MroXI GAANNNNTTC 1 cut(s) 1763
MscI TGGCCA 2 cut(s) 2158, 2309
MseI TTAA 9 cut(s) 114, 554, 698, 842, 864, 929, 1947, 2247, 2580
MslI CAYNNNNRTG 1 cut(s) 332
Msp20I TGGCCA 2 cut(s) 2158, 2309
MspA1I CMGCKG 2 cut(s) 962, 1677
MspI CCGG 4 cut(s) 675, 948, 1230, 2347
MspR9I CCNGG 6 cut(s) 10, 770, 1231, 1324, 1662, 2347
MunI CAATTG 2 cut(s) 1938, 2818
Mva1269I GAATGC 2 cut(s) 495, 1378
MvaI CCWGG 4 cut(s) 10, 770, 1324, 1662
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 4 cut(s) 19, 1281, 1475, 2164
NciI CCSGG 2 cut(s) 1231, 2347
NcoI CCATGG 1 cut(s) 29
NdeII GATC 9 cut(s) 211, 728, 988, 1542, 2172, 2229, 2557, 2706, 2713
NlaIV GGNNCC 5 cut(s) 51, 265, 286, 2376, 2559
NmuCI GTSAC 4 cut(s) 572, 1440, 1498, 2786
NruI TCGCGA 1 cut(s) 78
NspI RCATGY 3 cut(s) 307, 835, 943
NspV TTCGAA 1 cut(s) 2772
PagI TCATGA 1 cut(s) 136
PaqCI CACCTGC 2 cut(s) 1469, 1679
PceI AGGCCT 1 cut(s) 2650
PciI ACATGT 1 cut(s) 831
PciSI GCTCTTC 1 cut(s) 1075
PctI GAATGC 2 cut(s) 495, 1378
PdmI GAANNNNTTC 1 cut(s) 1763
PfeI GAWTC 8 cut(s) 182, 640, 734, 1354, 1628, 1704, 1978, 2216
PflMI CCANNNNNTGG 2 cut(s) 1046, 1667
PkrI GCNGC 5 cut(s) 36, 39, 810, 1477, 1676
PleI GAGTC 3 cut(s) 1335, 1727, 2805
PpsI GAGTC 3 cut(s) 1335, 1727, 2805
PscI ACATGT 1 cut(s) 831
PshAI GACNNNNGTC 1 cut(s) 1599
Psp6I CCWGG 4 cut(s) 8, 768, 1322, 1660
PspEI GGTNACC 1 cut(s) 2786
PspFI CCCAGC 2 cut(s) 1559, 1656
PspGI CCWGG 4 cut(s) 8, 768, 1322, 1660
PspN4I GGNNCC 5 cut(s) 51, 265, 286, 2376, 2559
PspPI GGNCC 8 cut(s) 662, 1232, 1601, 1772, 1901, 1915, 2126, 2548
PstI CTGCAG 1 cut(s) 1480
PstNI CAGNNNCTG 3 cut(s) 185, 1475, 2175
PsuI RGATCY 4 cut(s) 728, 1542, 2172, 2557
PvuII CAGCTG 1 cut(s) 962
RruI TCGCGA 1 cut(s) 78
RsaI GTAC 8 cut(s) 44, 308, 750, 1133, 1167, 1367, 1534, 2090
RsaNI GTAC 8 cut(s) 43, 307, 749, 1132, 1166, 1366, 1533, 2089
RseI CAYNNNNRTG 1 cut(s) 332
SalI GTCGAC 1 cut(s) 835
SapI GCTCTTC 1 cut(s) 1075
SaqAI TTAA 9 cut(s) 114, 554, 698, 842, 864, 929, 1947, 2247, 2580
SatI GCNGC 5 cut(s) 35, 38, 809, 1476, 1675
Sau3AI GATC 9 cut(s) 211, 728, 988, 1542, 2172, 2229, 2557, 2706, 2713
Sau96I GGNCC 8 cut(s) 662, 1232, 1601, 1772, 1901, 1915, 2126, 2548
ScaI AGTACT 1 cut(s) 2090
SchI GAGTC 3 cut(s) 1335, 1728, 2806
ScrFI CCNGG 6 cut(s) 10, 770, 1231, 1324, 1662, 2347
SduI GDGCHC 3 cut(s) 287, 2119, 2518
SfaNI GCATC 2 cut(s) 1277, 1462
SfcI CTRYAG 2 cut(s) 1476, 1623
SfuI TTCGAA 1 cut(s) 2772
SinI GGWCC 4 cut(s) 1601, 1772, 1901, 2548
SmiMI CAYNNNNRTG 1 cut(s) 332
SmlI CTYRAG 4 cut(s) 626, 1647, 1834, 2792
SmoI CTYRAG 4 cut(s) 626, 1647, 1834, 2792
SpeI ACTAGT 2 cut(s) 295, 634
SseBI AGGCCT 1 cut(s) 2650
SsiI CCGC 7 cut(s) 35, 247, 458, 1677, 1712, 1935, 2479
SspI AATATT 3 cut(s) 143, 799, 1465
StuI AGGCCT 1 cut(s) 2650
StyD4I CCNGG 6 cut(s) 8, 768, 1229, 1322, 1660, 2345
StyI CCWWGG 3 cut(s) 29, 202, 2561
TaaI ACNGT 4 cut(s) 54, 365, 578, 955
TaiI ACGT 1 cut(s) 1524
TaqI TCGA 6 cut(s) 426, 503, 836, 2268, 2772, 2800
TatI WGTACW 4 cut(s) 306, 748, 1365, 2088
TauI GCSGC 1 cut(s) 37
TfiI GAWTC 8 cut(s) 182, 640, 734, 1354, 1628, 1704, 1978, 2216
Tru1I TTAA 9 cut(s) 114, 554, 698, 842, 864, 929, 1947, 2247, 2580
Tru9I TTAA 9 cut(s) 114, 554, 698, 842, 864, 929, 1947, 2247, 2580
TscAI CASTG 4 cut(s) 1519, 1701, 2461, 2670
TseFI GTSAC 4 cut(s) 572, 1440, 1498, 2786
TseI GCWGC 4 cut(s) 37, 808, 1475, 1674
Tsp45I GTSAC 4 cut(s) 572, 1440, 1498, 2786
TspGWI ACGGA 1 cut(s) 1535
TspRI CASTG 4 cut(s) 1519, 1701, 2461, 2670
Van91I CCANNNNNTGG 2 cut(s) 1046, 1667
VpaK11BI GGWCC 4 cut(s) 1601, 1772, 1901, 2548
XagI CCTNNNNNAGG 2 cut(s) 1587, 2210
XapI RAATTY 6 cut(s) 469, 846, 1004, 1180, 1746, 1985
XceI RCATGY 3 cut(s) 307, 835, 943
XcmI CCANNNNNNNNNTGG 2 cut(s) 1664, 2662
XmiI GTMKAC 2 cut(s) 836, 1809
XmnI GAANNNNTTC 1 cut(s) 1763
ZrmI AGTACT 1 cut(s) 2090
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.