Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0000292

Physical Location & Seq

Genomic Coordinates Forward (+)

70420190 .. 70454482

Gene Structure 34293 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00050090.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2865 bp

ATGAACAACTACATGCTGTTGATCCATCGGGTTCTGGTTCATTCTCTCCTGCTACTTTGCTTTGCAACCTTGGCTTTCGGAGCTGATCGATTATCAGCCGATGAAGTACAAGTTTTGAAGGACATAGCAAAAACACTGGGGAAGACAAACTGGAATTTTAGTGCTGATCCATGCACCGGAGAATATGGATGGATTACTCAAGCCGACAAATATTTTGCGAATAACGTCACCTGCGGCAATTGTACGACTTCCGATGCGGCCAATGACACCAGAACTGATCAGATCTGCCATGTTGTTAGCATACTTCTGAGAGCTCAGAATTTGCCAGGAACACTTCCGTCAGAGTTGGCAAGGCTTCCCTATCTCCAACAAATTGACCTCGCCCGTAATTATCTGAATGGAACCATCCCTCCTCAATGGGGCTCCCTGCCACTAGTTAACATTTCTCTTGTTGGAAACCGTTTGACAGGTTCTATCCCCGTTGAGCTTGCAAATATTACCACCCTGAAAAGTTTGACCATGGATTTCAATTATTTCTCTGGAGGTCTTCCTTGGGAACTTGGATTTTTGCCGAGCATAGAAACACTGAGAATTACTGACTTGAATGGACCTGAATCATCCTTTCCTCTACTTGAGGATATGAAAAACTTGAAGATACTGATGTTGAGGAGTTGCAATATTAATGGACAGCTACCAGAATATTTTTCGGACATGACAGAGTTGACAACCTTAGACCTAAGCTTCAACAAGCTAACTGGAAATGTCCCAAGGAGCTTTGTTGCTCCAACGAATAATTTGGATTACATATTCTTAACTGGAAATTTGCTGAATGGATCTGAGCCTCTGTGGATGGTAGGAGAAAACGTTGATCTTTCATATAACAATTTCACTATCACCTCAGGGCCTGTAAATTGTCAGCCACAAGCCGGCTGGAACTTGTTTTCAAGCTCTTCAAAGGGCAACAGTTCAGCTGTTTCATGTTTGAGCAATAAAACTACAACATGTTCAAAAAGATGGTACTCTCTCAATATAAATTGTGGAGGAAGTGGAGTAACAATTGATGGAACAACATATGATGAGGATACAGTTGCAAATGGACCCTCATCATATTATATGAGCAGCACAAATTGGGTGTCAAGCAGCACTGGTTACTTCCCTGATGATGACAGTTCTACAGACCACTTTACTTGGTCCAATAATTCATCTAGACTCTCTATGGTCAATGGTCAACTATACACGACTGCGCGCCTTTCTCCCATCTCTCTAACTTATTATGGGTTTTGCCTGCTGAATGGAAACTACACAGTAAACCTCCATTTTGCAGAGATAATGTTTACAAATGGTCCAAGTCCAACATATAGCAGCTTGGGAAGGCGCATATTTGATGTTTACATTCAGGGCATACGAGTGCTGAAGGATTTCAATATTGCAGATGAGGCAGGTGGGGCTGGTAAGGCAGTCATAAAAGAGTATATTGCTGCTGTAACTACTAATACCTTGGAGATCCGTTTCTTTTGGGCTGGGAAAGGAACCACAGGTCTCCCTGTAAGAGGAGTTTATGGTCCTCTTATCTCCGCCATATCTGTGGTTCCAGACTTTGAACCCCCGGCAGAAACCCCGTCAGGACCCATATCAATGAGGTTATCTATAGGTGCAGTGGTTGGAAGTATGGCTGCAGGGGGAGTATTACTTGTATTGCTGGTTTTGGGTTATCTTTGGTGGAGAGGCTATCTAAGACGAGAAAGGACTTTGGAGCAAGATTTAAAAGGTGTTGACCTGCAAACGGGTAGATTTAGCTTGAGACAAATAAAAGATGCCACAGAAAACTTTGACATAGCCAACAAGATTGGAGAAGGTGGTTTTGGTCGTGTTTACAAGGGCCAACTATCAGATGGCACTGTAATTGCTGTTAAGCAACTTTCTGCCAAATCAAAGCAAGGAAATCGTGAATTTGTGAATGAGATTGGCATGATTTCTGCTCTGCAACACCCTCATCTTGTCAAGCTCTATGGATGCTGCATTGAAGGAAATCAGTTGTTGCTTGTCTATGAGTACATGGAAAATAACAGTGTTGGACGTGCCTTGTTTGGTGCAAAAGAAAGTCAGTTGAAGTTAGATTGGCCAACAAGGCACAGGATTTGTGTTGGTATAGCAAAAGGTTTGGCTTACCTTCATGAAGAATCACGGTTGAAGATCGTTCATAGAGACATCAAAGCTACTAATGTGCTACTTGATAAAAATCTTTACCCTAAGATATCCGACTTTGGCCTAGCCAAGCTTGTTGAAGATGATAGTACCCATATAAGCACTAGGATTGCTGGAACTCATGGATATATGTCACCTGAATACGCAATGCGGGGTTATCTGACTGATAAAGCAGATGTGTATAGTTTCGGAATTCTTGTGTTGGAAATGGTCAATGGAAGGAGCAACACAACTTACCACTCCAAGGGAGAGTGCTACTACATTCTTGATTGGGCACTTCTTCTAAAAGAGAAAGGAAGTTTGTTAGACCTAGTCGATCCAAGGTTGGGCTCAGATTTTAACAAAGCAGACGTGATGATTACCATCAATGTGGCTCTCCTGTGCTGTAATGTCTCTGCAGCACTTAGGCCTGCCATGTCTTCAGTGGTGAGCATGCTTGAAGGTAGGGCTCGTGTTGAGGAATTAGTCTTAGATTCAAGTGCCTCATCTCATGAGATCAGTGAAACAAGGAAACATTTTGAGTCCATTTACTCATTGGAGACCATCGCTGGGAGTACTCAGAGACAAACGATGTCAATCGAAGAGGCATGCACTTCACCGACATCTGTTCAAGATCATCCAATCAATCCTCATTCAGATTACTGGGAGAACAGAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0002682 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009814 GO:0009861 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030054 GO:0031347 GO:0036211 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0045088 GO:0048583 GO:0050776 GO:0050789 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_1 LRR_8 Malectin Pkinase Pkinase_Tyr

Protein Analysis

954

Amino Acids

105.15

Weight (kDa)

5.68

Isoelectric Point (pI)

35.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_8	PF13855	216 - 276	7.8e-08	Leucine rich repeat
Malectin	PF11721	341 - 527	6.3e-43	Malectin domain
PK_Tyr_Ser-Thr	PF07714	612 - 812	5.7e-47	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	612 - 813	2.7e-46	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000292)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53420 AT3G14840
fragaria_vesca	FvH4_3g17840 FvH4_3g17840 FvH4_3g17840 FvH4_3g17840 FvH4_3g17840 FvH4_3g17840 FvH4_3g17841 FvH4_3g17841 FvH4_3g17841 FvH4_3g17841 FvH4_3g17841 FvH4_3g17841 FvH4_3g17850 FvH4_3g17850 FvH4_3g17850 FvH4_3g17850 FvH4_3g17851
malus_domestica	MD03G1246600.v1.1 MD06G1045200.v1.1 MD06G1045400.v1.1 MD11G1267800.v1.1 MD11G1267900.v1.1 MD11G1268100.v1.1 MD11G1268300.v1.1 MD11G1268500.v1.1 MD11G1268600.v1.1
prunus_persica	Prupe.4G157700_v2.0.a1 Prupe.4G157700_v2.0.a1 Prupe.4G157800_v2.0.a1 Prupe.4G157800_v2.0.a1 Prupe.4G157800_v2.0.a1 Prupe.4G157800_v2.0.a1 Prupe.4G157800_v2.0.a1 Prupe.4G157900_v2.0.a1 Prupe.4G157900_v2.0.a1 Prupe.4G157900_v2.0.a1
pyrus_communis	pycom03g19540 pycom11g23600 pycom11g23630 pycom11g23690 pycom11g23720 pycom11g23730 pycom11g23740 pycom11g23750
rosa_chinensis	RchiOBHm_Chr0c40g0503411 RchiOBHm_Chr0c40g0503421 RchiOBHm_Chr5g0029631 RchiOBHm_Chr5g0029661 RchiOBHm_Chr5g0029701 RchiOBHm_Chr5g0029741 RchiOBHm_Chr5g0029771 RchiOBHm_Chr5g0029781 RchiOBHm_Chr5g0029811 RchiOBHm_Chr5g0029851 RchiOBHm_Chr5g0029871 RchiOBHm_Chr5g0029891 RchiOBHm_Chr7g0222221
rosa_laevigata	RLG00000017380 RLG00000032873 RLG00000033188 RLG00000033190 RLG00000033192 RLG00000033193 RLG00000033194 RLG00000033196 RLG00000033199 RLG00000033238
rosa_multiflora	Rmu_co8359987.1_g000001 Rmu_co8444909.1_g000001 Rmu_sc0001608.1_g000007 Rmu_sc0001608.1_g000009 Rmu_sc0001608.1_g000013 Rmu_sc0001608.1_g000018 Rmu_sc0001608.1_g000023 Rmu_sc0001608.1_g000040 Rmu_sc0003238.1_g000016 Rmu_sc0003368.1_g000026 Rmu_sc0003368.1_g000034 Rmu_sc0003368.1_g000043 Rmu_sc0003368.1_g000047 Rmu_sc0003368.1_g000052 Rmu_sc0003368.1_g000054 Rmu_sc0004963.1_g000001
rosa_roxburghii	Rroxscaffold_1G00050080 Rroxscaffold_1G00050090 Rroxscaffold_1G00050110 Rroxscaffold_1G00050120 Rroxscaffold_1G00050180 Rroxscaffold_1G00050190 Rroxscaffold_1G00050220 Rroxscaffold_1G00050270 Rroxscaffold_1G00050300
rosa_rugosa	Rorug04G0065200 Rorug05G0114900 Rorug05G0115000 Rorug05G0115100 Rorug05G0120100 Rorug05G0238900
rosa_samantha	Rh5AG207800 Rh5AG208000 Rh5AG208300 Rh5AG208400 Rh5AG208800 Rh5AG294900 Rh5AG295000 Rh5AG295400 Rh5CG230400 Rh7AG347800
rosa_wichuraiana	Rw0G005370 Rw0G012460 Rw0G019060 Rw5G018910 Rw5G018920 Rw5G018930 Rw5G018940 Rw5G018960 Rw5G018970 Rw5G018980 Rw5G018990 Rw5G019000 Rw7G029840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	239, 1430
Acc36I	ACCTGC	3 cut(s)	239, 1430, 1785
AccII	CGCG	1 cut(s)	1246
AciI	CCGC	4 cut(s)	234, 257, 1575, 2356
AclI	AACGTT	1 cut(s)	864
AclWI	GGATC	5 cut(s)	16, 161, 841, 1498, 2516
AcoI	YGGCCR	2 cut(s)	258, 2120
AcsI	RAATTY	5 cut(s)	154, 319, 820, 1949, 2397
AcuI	CTGAAG	2 cut(s)	1433, 2610
AfaI	GTAC	6 cut(s)	108, 244, 1019, 2054, 2296, 2761
AfiI	CCNNNNNNNGG	8 cut(s)	176, 419, 926, 1550, 1783, 2449, 2531, 2754
AflIII	ACRYGT	1 cut(s)	1001
AhlI	ACTAGT	1 cut(s)	433
AjiI	CACGTC	2 cut(s)	2078, 2557
AjnI	CCWGG	1 cut(s)	325
AjuI	GAANNNNNNNTTGG	2 cut(s)	1845, 1877
AloI	GAACNNNNNNTCC	2 cut(s)	394, 426
Alw21I	GWGCWC	1 cut(s)	316
Alw26I	GTCTC	6 cut(s)	1544, 1795, 2199, 2602, 2738, 2761
AlwI	GGATC	5 cut(s)	16, 161, 841, 1498, 2516
AlwNI	CAGNNNCTG	1 cut(s)	905
AoxI	GGCC	6 cut(s)	258, 902, 1879, 2120, 2266, 2612
ApeKI	GCWGC	7 cut(s)	1119, 1140, 1362, 1478, 1673, 2016, 2603
ApoI	RAATTY	5 cut(s)	154, 319, 820, 1949, 2397
ArsI	GACNNNNNNTTYG	4 cut(s)	197, 229, 1118, 1150
AseI	ATTAAT	1 cut(s)	681
Asp700I	GAANNNNTTC	1 cut(s)	1418
AspLEI	GCGC	3 cut(s)	1246, 1248, 1377
AspS9I	GGNCC	8 cut(s)	608, 902, 1097, 1191, 1343, 1562, 1625, 1879
AsuC2I	CCSGG	1 cut(s)	1607
AsuHPI	GGTGA	5 cut(s)	220, 886, 2331, 2644, 2793
AvaII	GGWCC	6 cut(s)	608, 1097, 1191, 1343, 1562, 1625
AxyI	CCTNAGG	1 cut(s)	898
BaeGI	GKGCMC	1 cut(s)	2482
BaeI	ACNNNNGTAYC	2 cut(s)	647, 680
BalI	TGGCCA	1 cut(s)	2122
BanII	GRGCYC	4 cut(s)	316, 425, 2537, 2656
BauI	CACGAG	1 cut(s)	2655
BbsI	GAAGAC	3 cut(s)	149, 539, 2616
Bbv12I	GWGCWC	1 cut(s)	316
BbvI	GCAGC	7 cut(s)	1131, 1152, 1374, 1465, 1660, 2003, 2615
BcgI	CGANNNNNNTGC	2 cut(s)	1925, 1959
BciT130I	CCWGG	1 cut(s)	327
BciVI	GTATCC	1 cut(s)	1075
BclI	TGATCA	1 cut(s)	277
BcnI	CCSGG	1 cut(s)	1607
BcoDI	GTCTC	6 cut(s)	1544, 1795, 2199, 2602, 2738, 2761
BcuI	ACTAGT	1 cut(s)	433
BfaI	CTAG	5 cut(s)	434, 1206, 2270, 2310, 2516
BfmI	CTRYAG	4 cut(s)	1173, 1647, 1674, 2601
BfuAI	ACCTGC	3 cut(s)	239, 1430, 1785
BfuI	GTATCC	1 cut(s)	1075
BglI	GCCNNNNNGGC	1 cut(s)	2128
BglII	AGATCT	1 cut(s)	282
BisI	GCNGC	9 cut(s)	235, 258, 1120, 1141, 1363, 1479, 1674, 2017, 2604
BlsI	GCNGC	9 cut(s)	236, 259, 1121, 1142, 1364, 1480, 1675, 2018, 2605
BmcAI	AGTACT	1 cut(s)	2761
Bme1390I	CCNGG	2 cut(s)	327, 1607
Bme18I	GGWCC	6 cut(s)	608, 1097, 1191, 1343, 1562, 1625
BmgBI	CACGTC	2 cut(s)	2078, 2557
BmgT120I	GGNCC	8 cut(s)	608, 902, 1097, 1191, 1343, 1562, 1625, 1879
BmiI	GGNNCC	6 cut(s)	403, 424, 1099, 1531, 1590, 1627
BmrFI	CCNGG	2 cut(s)	327, 1607
BmrI	ACTGGG	2 cut(s)	146, 2857
BmsI	GCATC	3 cut(s)	244, 1804, 2003
BmuI	ACTGGG	2 cut(s)	146, 2857
BpiI	GAAGAC	3 cut(s)	149, 539, 2616
BpmI	CTGGAG	1 cut(s)	561
Bpu10I	CCTNAGC	1 cut(s)	737
BpuEI	CTTGAG	3 cut(s)	183, 653, 1819
BpuMI	CCSGG	1 cut(s)	1607
Bsa29I	ATCGAT	1 cut(s)	88
BsaBI	GATNNNNATC	3 cut(s)	2238, 2567, 2780
BsaI	GGTCTC	2 cut(s)	1544, 2738
BsaJI	CCNNGG	8 cut(s)	69, 519, 551, 767, 1497, 1605, 2448, 2525
BsaWI	WCCGGW	1 cut(s)	176
BsaXI	ACNNNNNCTCC	4 cut(s)	394, 424, 849, 879
Bsc4I	CCNNNNNNNGG	8 cut(s)	176, 419, 926, 1550, 1783, 2449, 2531, 2754
Bse118I	RCCGGY	1 cut(s)	926
Bse1I	ACTGG	6 cut(s)	141, 155, 760, 820, 1150, 2852
Bse21I	CCTNAGG	1 cut(s)	898
Bse3DI	GCAATG	1 cut(s)	2358
Bse8I	GATNNNNATC	3 cut(s)	2238, 2567, 2780
BseBI	CCWGG	1 cut(s)	327
BseCI	ATCGAT	1 cut(s)	88
BseDI	CCNNGG	8 cut(s)	69, 519, 551, 767, 1497, 1605, 2448, 2525
BseGI	GGATG	6 cut(s)	194, 405, 617, 855, 2018, 2821
BseJI	GATNNNNATC	3 cut(s)	2238, 2567, 2780
BseLI	CCNNNNNNNGG	8 cut(s)	176, 419, 926, 1550, 1783, 2449, 2531, 2754
BseMI	GCAATG	1 cut(s)	2358
BseMII	CTCAG	7 cut(s)	299, 329, 578, 828, 912, 2550, 2777
BseNI	ACTGG	6 cut(s)	141, 155, 760, 820, 1150, 2852
BsePI	GCGCGC	1 cut(s)	1244
BseRI	GAGGAG	3 cut(s)	402, 682, 1566
BseSI	GKGCMC	1 cut(s)	2482
BseXI	GCAGC	7 cut(s)	1131, 1152, 1374, 1465, 1660, 2003, 2615
BseYI	CCCAGC	2 cut(s)	1520, 2753
BsgI	GTGCAG	1 cut(s)	1674
Bsh1236I	CGCG	1 cut(s)	1246
BshFI	GGCC	6 cut(s)	260, 904, 1881, 2122, 2268, 2614
BshVI	ATCGAT	1 cut(s)	88
BsiHKAI	GWGCWC	1 cut(s)	316
BsiSI	CCGG	3 cut(s)	177, 927, 1607
BslFI	GGGAC	1 cut(s)	749
BslI	CCNNNNNNNGG	8 cut(s)	176, 419, 926, 1550, 1783, 2449, 2531, 2754
BsmAI	GTCTC	6 cut(s)	1544, 1795, 2199, 2602, 2738, 2761
BsmFI	GGGAC	1 cut(s)	749
BsnI	GGCC	6 cut(s)	260, 904, 1881, 2122, 2268, 2614
Bso31I	GGTCTC	2 cut(s)	1544, 2738
Bsp1286I	GDGCHC	5 cut(s)	316, 425, 2482, 2537, 2656
Bsp19I	CCATGG	1 cut(s)	519
BspACI	CCGC	4 cut(s)	234, 257, 1575, 2356
BspANI	GGCC	6 cut(s)	260, 904, 1881, 2122, 2268, 2614
BspCNI	CTCAG	7 cut(s)	300, 328, 579, 829, 911, 2549, 2776
BspDI	ATCGAT	1 cut(s)	88
BspFNI	CGCG	1 cut(s)	1246
BspHI	TCATGA	2 cut(s)	2173, 2695
BspLI	GGNNCC	6 cut(s)	403, 424, 1099, 1531, 1590, 1627
BspMAI	CTGCAG	2 cut(s)	1678, 2605
BspMI	ACCTGC	3 cut(s)	239, 1430, 1785
BspPI	GGATC	5 cut(s)	16, 161, 841, 1498, 2516
BspQI	GCTCTTC	1 cut(s)	955
BspTNI	GGTCTC	2 cut(s)	1544, 2738
BsrDI	GCAATG	1 cut(s)	2358
BsrFI	RCCGGY	1 cut(s)	926
BsrI	ACTGG	6 cut(s)	141, 155, 760, 820, 1150, 2852
BssAI	RCCGGY	1 cut(s)	926
BssECI	CCNNGG	8 cut(s)	69, 519, 551, 767, 1497, 1605, 2448, 2525
BssHII	GCGCGC	1 cut(s)	1244
BssSI	CACGAG	1 cut(s)	2655
BssT1I	CCWWGG	7 cut(s)	69, 519, 551, 767, 1497, 2448, 2525
Bst2BI	CACGAG	1 cut(s)	2655
Bst2UI	CCWGG	1 cut(s)	327
Bst4CI	ACNGT	8 cut(s)	461, 965, 1087, 1169, 1306, 1900, 2069, 2187
Bst6I	CTCTTC	2 cut(s)	955, 2781
BstC8I	GCNNGC	7 cut(s)	489, 928, 1246, 1286, 2616, 2639, 2794
BstDSI	CCRYGG	1 cut(s)	519
BstENI	CCTNNNNNAGG	1 cut(s)	1548
BstF5I	GGATG	6 cut(s)	194, 405, 617, 855, 2018, 2821
BstFNI	CGCG	1 cut(s)	1246
BstHHI	GCGC	3 cut(s)	1246, 1248, 1377
BstMAI	GTCTC	6 cut(s)	1544, 1795, 2199, 2602, 2738, 2761
BstMWI	GCNNNNNNNGC	6 cut(s)	71, 80, 1436, 1445, 1454, 2128
BstNI	CCWGG	1 cut(s)	327
BstNSI	RCATGY	4 cut(s)	16, 1005, 2641, 2796
BstSCI	CCNGG	2 cut(s)	325, 1605
BstSFI	CTRYAG	4 cut(s)	1173, 1647, 1674, 2601
BstSLI	GKGCMC	1 cut(s)	2482
BstUI	CGCG	1 cut(s)	1246
BstV1I	GCAGC	7 cut(s)	1131, 1152, 1374, 1465, 1660, 2003, 2615
BstV2I	GAAGAC	3 cut(s)	149, 539, 2616
BstX2I	RGATCY	3 cut(s)	282, 833, 1503
BstXI	CCANNNNNNTGG	2 cut(s)	1585, 2575
BstYI	RGATCY	3 cut(s)	282, 833, 1503
Bsu15I	ATCGAT	1 cut(s)	88
Bsu36I	CCTNAGG	1 cut(s)	898
BsuI	GTATCC	1 cut(s)	1075
BsuRI	GGCC	6 cut(s)	260, 904, 1881, 2122, 2268, 2614
BsuTUI	ATCGAT	1 cut(s)	88
BtgI	CCRYGG	1 cut(s)	519
BtgZI	GCGATG	1 cut(s)	2734
BtrI	CACGTC	2 cut(s)	2078, 2557
BtsCI	GGATG	6 cut(s)	194, 405, 617, 855, 2018, 2821
BtsI	GCAGTG	1 cut(s)	1662
BtsIMutI	CAGTG	8 cut(s)	134, 584, 1143, 1662, 1896, 2074, 2634, 2710
BveI	ACCTGC	3 cut(s)	239, 1430, 1785
Cac8I	GCNNGC	7 cut(s)	489, 928, 1246, 1286, 2616, 2639, 2794
CaiI	CAGNNNCTG	1 cut(s)	905
CciI	TCATGA	2 cut(s)	2173, 2695
CfoI	GCGC	3 cut(s)	1246, 1248, 1377
Cfr10I	RCCGGY	1 cut(s)	926
Cfr13I	GGNCC	8 cut(s)	608, 902, 1097, 1191, 1343, 1562, 1625, 1879
ClaI	ATCGAT	1 cut(s)	88
Csp6I	GTAC	6 cut(s)	107, 243, 1018, 2053, 2295, 2760
CspCI	CAANNNNNGTGG	2 cut(s)	1169, 1204
CviQI	GTAC	6 cut(s)	107, 243, 1018, 2053, 2295, 2760
DraI	TTTAAA	1 cut(s)	1764
EaeI	YGGCCR	2 cut(s)	258, 2120
Eam1104I	CTCTTC	2 cut(s)	955, 2781
EarI	CTCTTC	2 cut(s)	955, 2781
EciI	GGCGGA	1 cut(s)	1564
Ecl136II	GAGCTC	1 cut(s)	314
Eco130I	CCWWGG	7 cut(s)	69, 519, 551, 767, 1497, 2448, 2525
Eco147I	AGGCCT	1 cut(s)	2614
Eco24I	GRGCYC	4 cut(s)	316, 425, 2537, 2656
Eco31I	GGTCTC	2 cut(s)	1544, 2738
Eco32I	GATATC	1 cut(s)	2256
Eco47I	GGWCC	6 cut(s)	608, 1097, 1191, 1343, 1562, 1625
Eco53kI	GAGCTC	1 cut(s)	314
Eco57I	CTGAAG	2 cut(s)	1433, 2610
Eco81I	CCTNAGG	1 cut(s)	898
EcoICRI	GAGCTC	1 cut(s)	314
EcoNI	CCTNNNNNAGG	1 cut(s)	1548
EcoO109I	RGGNCCY	2 cut(s)	902, 1625
EcoRI	GAATTC	1 cut(s)	2397
EcoRII	CCWGG	1 cut(s)	325
EcoRV	GATATC	1 cut(s)	2256
EcoT14I	CCWWGG	7 cut(s)	69, 519, 551, 767, 1497, 2448, 2525
EcoT38I	GRGCYC	4 cut(s)	316, 425, 2537, 2656
ErhI	CCWWGG	7 cut(s)	69, 519, 551, 767, 1497, 2448, 2525
FaqI	GGGAC	1 cut(s)	749
FauI	CCCGC	1 cut(s)	2349
FauNDI	CATATG	1 cut(s)	1072
FbaI	TGATCA	1 cut(s)	277
Fnu4HI	GCNGC	9 cut(s)	235, 258, 1120, 1141, 1363, 1479, 1674, 2017, 2604
FokI	GGATG	6 cut(s)	201, 392, 604, 862, 2025, 2808
FriOI	GRGCYC	4 cut(s)	316, 425, 2537, 2656
Fsp4HI	GCNGC	9 cut(s)	235, 258, 1120, 1141, 1363, 1479, 1674, 2017, 2604
FspBI	CTAG	5 cut(s)	434, 1206, 2270, 2310, 2516
GlaI	GCGC	3 cut(s)	1245, 1247, 1376
GluI	GCNGC	9 cut(s)	235, 258, 1120, 1141, 1363, 1479, 1674, 2017, 2604
GsaI	CCCAGC	2 cut(s)	1524, 2757
GsuI	CTGGAG	1 cut(s)	561
HaeIII	GGCC	6 cut(s)	260, 904, 1881, 2122, 2268, 2614
HapII	CCGG	3 cut(s)	177, 927, 1607
HhaI	GCGC	3 cut(s)	1246, 1248, 1377
Hin6I	GCGC	3 cut(s)	1244, 1246, 1375
HinP1I	GCGC	3 cut(s)	1244, 1246, 1375
HincII	GTYRAC	4 cut(s)	439, 723, 1229, 1774
HindII	GTYRAC	4 cut(s)	439, 723, 1229, 1774
HindIII	AAGCTT	2 cut(s)	739, 2276
HinfI	GANTC	5 cut(s)	614, 1209, 2180, 2678, 2726
HpaI	GTTAAC	1 cut(s)	439
HpaII	CCGG	3 cut(s)	177, 927, 1607
HphI	GGTGA	5 cut(s)	220, 886, 2331, 2644, 2793
Hpy166II	GTNNAC	8 cut(s)	439, 723, 1229, 1309, 1335, 1390, 1774, 1873
Hpy188III	TCNNGA	9 cut(s)	540, 1206, 1592, 1623, 1946, 2174, 2471, 2696, 2816
Hpy8I	GTNNAC	8 cut(s)	439, 723, 1229, 1309, 1335, 1390, 1774, 1873
HpyAV	CCTTC	8 cut(s)	112, 1365, 1408, 1847, 2018, 2180, 2418, 2639
HpyCH4III	ACNGT	8 cut(s)	461, 965, 1087, 1169, 1306, 1900, 2069, 2187
HpyCH4IV	ACGT	4 cut(s)	225, 864, 2077, 2556
HpyF10VI	GCNNNNNNNGC	6 cut(s)	71, 80, 1436, 1445, 1454, 2128
HpySE526I	ACGT	4 cut(s)	225, 864, 2077, 2556
HspAI	GCGC	3 cut(s)	1244, 1246, 1375
KroI	GCCGGC	1 cut(s)	926
KroNI	GCCGGC	1 cut(s)	928
Ksp22I	TGATCA	1 cut(s)	277
KspAI	GTTAAC	1 cut(s)	439
LguI	GCTCTTC	1 cut(s)	955
LmnI	GCTCC	6 cut(s)	80, 428, 771, 787, 1753, 2427
Lsp1109I	GCAGC	7 cut(s)	1131, 1152, 1374, 1465, 1660, 2003, 2615
LweI	GCATC	3 cut(s)	244, 1804, 2003
MaeI	CTAG	5 cut(s)	434, 1206, 2270, 2310, 2516
MaeII	ACGT	4 cut(s)	225, 864, 2077, 2556
MaeIII	GTNAC	5 cut(s)	226, 1051, 1148, 1483, 2337
MfeI	CAATTG	2 cut(s)	238, 1056
MflI	RGATCY	3 cut(s)	282, 833, 1503
MhlI	GDGCHC	5 cut(s)	316, 425, 2482, 2537, 2656
MlsI	TGGCCA	1 cut(s)	2122
MluNI	TGGCCA	1 cut(s)	2122
MlyI	GAGTC	2 cut(s)	1203, 2735
MmeI	TCCRAC	8 cut(s)	391, 433, 809, 1376, 1642, 2053, 2283, 2388
Mox20I	TGGCCA	1 cut(s)	2122
MroNI	GCCGGC	1 cut(s)	926
MroXI	GAANNNNTTC	1 cut(s)	1418
MscI	TGGCCA	1 cut(s)	2122
MseI	TTAA	7 cut(s)	438, 681, 812, 1763, 1911, 2544, 2863
MslI	CAYNNNNRTG	4 cut(s)	1406, 1583, 1634, 2573
Msp20I	TGGCCA	1 cut(s)	2122
MspA1I	CMGCKG	1 cut(s)	971
MspI	CCGG	3 cut(s)	177, 927, 1607
MspR9I	CCNGG	2 cut(s)	327, 1607
MunI	CAATTG	2 cut(s)	238, 1056
MvaI	CCWGG	1 cut(s)	327
MvnI	CGCG	1 cut(s)	1246
MwoI	GCNNNNNNNGC	6 cut(s)	71, 80, 1436, 1445, 1454, 2128
NaeI	GCCGGC	1 cut(s)	928
NciI	CCSGG	1 cut(s)	1607
NcoI	CCATGG	1 cut(s)	519
NdeI	CATATG	1 cut(s)	1072
NgoMIV	GCCGGC	1 cut(s)	926
NlaIV	GGNNCC	6 cut(s)	403, 424, 1099, 1531, 1590, 1627
NmeAIII	GCCGAG	1 cut(s)	597
NmuCI	GTSAC	2 cut(s)	226, 2337
NspI	RCATGY	4 cut(s)	16, 1005, 2641, 2796
PaeI	GCATGC	2 cut(s)	2641, 2796
PagI	TCATGA	2 cut(s)	2173, 2695
PaqCI	CACCTGC	2 cut(s)	239, 1430
PauI	GCGCGC	1 cut(s)	1244
PceI	AGGCCT	1 cut(s)	2614
PciI	ACATGT	1 cut(s)	1001
PciSI	GCTCTTC	1 cut(s)	955
PdiI	GCCGGC	1 cut(s)	928
PdmI	GAANNNNTTC	1 cut(s)	1418
PfeI	GAWTC	3 cut(s)	614, 2180, 2678
PflFI	GACNNNGTC	1 cut(s)	2516
PkrI	GCNGC	9 cut(s)	236, 259, 1121, 1142, 1364, 1480, 1675, 2018, 2605
PleI	GAGTC	2 cut(s)	1203, 2734
PpsI	GAGTC	2 cut(s)	1203, 2734
PpuMI	RGGWCCY	1 cut(s)	1625
PscI	ACATGT	1 cut(s)	1001
PshBI	ATTAAT	1 cut(s)	681
Psp124BI	GAGCTC	1 cut(s)	316
Psp1406I	AACGTT	1 cut(s)	864
Psp5II	RGGWCCY	1 cut(s)	1625
Psp6I	CCWGG	1 cut(s)	325
PspFI	CCCAGC	2 cut(s)	1520, 2753
PspGI	CCWGG	1 cut(s)	325
PspN4I	GGNNCC	6 cut(s)	403, 424, 1099, 1531, 1590, 1627
PspPI	GGNCC	8 cut(s)	608, 902, 1097, 1191, 1343, 1562, 1625, 1879
PspPPI	RGGWCCY	1 cut(s)	1625
PstI	CTGCAG	2 cut(s)	1678, 2605
PstNI	CAGNNNCTG	1 cut(s)	905
PsuI	RGATCY	3 cut(s)	282, 833, 1503
PsyI	GACNNNGTC	1 cut(s)	2516
PteI	GCGCGC	1 cut(s)	1244
PvuII	CAGCTG	1 cut(s)	971
RsaI	GTAC	6 cut(s)	108, 244, 1019, 2054, 2296, 2761
RsaNI	GTAC	6 cut(s)	107, 243, 1018, 2053, 2295, 2760
RseI	CAYNNNNRTG	4 cut(s)	1406, 1583, 1634, 2573
SacI	GAGCTC	1 cut(s)	316
SapI	GCTCTTC	1 cut(s)	955
SaqAI	TTAA	7 cut(s)	438, 681, 812, 1763, 1911, 2544, 2863
SatI	GCNGC	9 cut(s)	235, 258, 1120, 1141, 1363, 1479, 1674, 2017, 2604
Sau96I	GGNCC	8 cut(s)	608, 902, 1097, 1191, 1343, 1562, 1625, 1879
ScaI	AGTACT	1 cut(s)	2761
SchI	GAGTC	2 cut(s)	1203, 2735
ScrFI	CCNGG	2 cut(s)	327, 1607
SduI	GDGCHC	5 cut(s)	316, 425, 2482, 2537, 2656
SfaNI	GCATC	3 cut(s)	244, 1804, 2003
SfcI	CTRYAG	4 cut(s)	1173, 1647, 1674, 2601
SinI	GGWCC	6 cut(s)	608, 1097, 1191, 1343, 1562, 1625
SmiMI	CAYNNNNRTG	4 cut(s)	1406, 1583, 1634, 2573
SmlI	CTYRAG	3 cut(s)	198, 632, 1798
SmoI	CTYRAG	3 cut(s)	198, 632, 1798
SpeI	ACTAGT	1 cut(s)	433
SphI	GCATGC	2 cut(s)	2641, 2796
SseBI	AGGCCT	1 cut(s)	2614
SsiI	CCGC	4 cut(s)	234, 257, 1575, 2356
SspI	AATATT	5 cut(s)	212, 496, 679, 701, 1426
SspMI	CTAG	5 cut(s)	434, 1206, 2270, 2310, 2516
SstI	GAGCTC	1 cut(s)	316
StuI	AGGCCT	1 cut(s)	2614
StyD4I	CCNGG	2 cut(s)	325, 1605
StyI	CCWWGG	7 cut(s)	69, 519, 551, 767, 1497, 2448, 2525
TaaI	ACNGT	8 cut(s)	461, 965, 1087, 1169, 1306, 1900, 2069, 2187
TaiI	ACGT	4 cut(s)	228, 867, 2080, 2559
TaqI	TCGA	3 cut(s)	88, 2520, 2784
TatI	WGTACW	3 cut(s)	106, 2052, 2759
TauI	GCSGC	2 cut(s)	237, 260
TfiI	GAWTC	3 cut(s)	614, 2180, 2678
Tru1I	TTAA	7 cut(s)	438, 681, 812, 1763, 1911, 2544, 2863
Tru9I	TTAA	7 cut(s)	438, 681, 812, 1763, 1911, 2544, 2863
TscAI	CASTG	8 cut(s)	141, 591, 1150, 1662, 1903, 2074, 2634, 2710
TseFI	GTSAC	2 cut(s)	226, 2337
TseI	GCWGC	7 cut(s)	1119, 1140, 1362, 1478, 1673, 2016, 2603
Tsp45I	GTSAC	2 cut(s)	226, 2337
TspGWI	ACGGA	2 cut(s)	327, 1496
TspRI	CASTG	8 cut(s)	141, 591, 1150, 1662, 1903, 2074, 2634, 2710
Tth111I	GACNNNGTC	1 cut(s)	2516
VpaK11BI	GGWCC	6 cut(s)	608, 1097, 1191, 1343, 1562, 1625
VspI	ATTAAT	1 cut(s)	681
XagI	CCTNNNNNAGG	1 cut(s)	1548
XapI	RAATTY	5 cut(s)	154, 319, 820, 1949, 2397
XbaI	TCTAGA	1 cut(s)	1205
XceI	RCATGY	4 cut(s)	16, 1005, 2641, 2796
XcmI	CCANNNNNNNNNTGG	4 cut(s)	927, 1889, 2626, 2737
XmnI	GAANNNNTTC	1 cut(s)	1418
XspI	CTAG	5 cut(s)	434, 1206, 2270, 2310, 2516
ZrmI	AGTACT	1 cut(s)	2761

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.