Rw5G018970

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000292
Physical Location & Seq
Reverse (-)
24588221 .. 24604415
16195 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G018970.1

Sequence Viewer

Length: 2865 bp
ATGAATTACTTGTTCTTATTCTCTCAGCTTCTTATTGGTACCCATCTCTTGCTAGTTTTCTTTGCGAGCTTTGCTTTCGGAGCTAATCGATTATCTTCAGATGAAATACAAGCTTTAAGGGACATTGGAAAGACACTCGGTAAGAACTGGAATTTCAGCGTAGATCCATGCAGCGGAGAACTTGGATGGAGTACTGCTGCGGATGGCCAATACATGAATAACGTCACCTGCGGCAACTGTAGTACTACCTCCAATACTACGGCCGATCAGATCTGCCATGTCACAAACATACTTTTGAAAGCGCAGAATCTGACAGGACAACTCCCGAAGGAGCTGATCAACCTCCCCTATATCCAAGAAATTGACCTCACCCGCAACTACCTGAATGGCACCATCCCTCCAGAATGGGGCTCCCTGCCACTAGTTAGCATTTCTCTTGTTGGAAACCGTTTGACAGGTTCTATCCCTGTTGAGCTAGGAAACATCACCACGCTCAAAAGTTTGACCATCGATTTCAATCGTTTATCTGGAGTTCTTCCTGCGGAGCTTGGAAATTTGTCTAGCATAGAAAGAATGCTTCTTACCTCGAACAATTTTACTGGGAGTTGCCAGAAACATTTTCGGATTAGTGACAGTTCCTTTTCTGGGAAGATACCTGATTTTATTCAGAGCTGGACAAATCTTGAGGGACTAGTGATTCAGGCTAGTGGTTTTACAGGGCCAATTCCTTCCGGCATTTCTCTCTTGACAAGATTAACTGACCTGAGAATTACTGACTTGAATGGATCTGAATCATCCTTTCCTGTACTTGATAATATGAAAAGCCTGGAGACATTGGTGTTGCGGAACTGCAATATTAGTGGGCAGCTACCTGAATATCTTGGGAACATGTCGACTTTAAAAATTTTAGACCTTAGTTTTAACAAGCTAACTGGAGATATCCCAAACAACTTTGCTGGTCTAGGAAATCTGGATAATATGTTCTTAACTGGAAACATGCTGACCGGAACTGTGCCAGATGATTGGATGACAGCTGATTGGATGAAAGAAAAGAGCATTGATCTTTCATATAACAAATTCACCATCAACTCAAATGATGAGAATTGTGAACCACGAGATGGCTTGAACTTGTTTGCAAGCTCTTCAGGTGGCAATACTTCATTTGTTTCATGTTTACCACTTGCAAAATGTGTAAAAACTTGGTACACTCTTCATATAAATTGTGGTGGAAAAGAAGTACCCCTCTATGAAAATTTCACAAGACCCACCAACGCAACATACGAGGAAGATACTGATGTAGCCGGGCCTTCATCAGTTTACCTGAGCAGGACAAATTGGGCATCAAGCACGACTGGCTACTTCGCTGATGATAATCGTCTTACTAAAACCTATACCTGGACTAATTCATCTGGACTCAATATGGCGAATCCTGAGTTGTACATGAATGCACGCCTTTCTCCTATTTCTCTAACTTACTATGGGTTTTGTCTCGGAAATGGAAGCTACACAGTAAACCTCCACTTTGCAGAGATAATATTTACAAATGACACAACATATAGTAGCCTGGGAAGACGTGTATTCGATATATACATTCAGGGGTCACTAGCGTTCAAGGATTTCAATATTGCAGATGCTGCAGGTGGCTTTGGAAACGCAGTCACAAAAAAGTTCACTGCTAACGTAACTGCTGCAGGTACTTTGGAGATCCGTTTATTCTGGGCTGGGAAAGGGACAACAGGTGTTCCTTACAGAGGAGACTATGGTCCTCTTATATCAGCTATCTCCATAGATTCTGACTTTCCACCCCCTCAAGAAACCCAGCCAGGAGGTGGAAGCAGCGGTATATCAGCAGGTGCAGTGGTTGGAATCGTGGCTGGAGGAGTCTTCATTATATTACTGGTTTTAGGAATTCTCTGGTGGAAAGGGTTCATAGGACCAGCAAATACTTTAGAACAAGATTTGAAGGGTGTGGACCTGCAAACCAGCAGATTTACCTTGAGGCAAATCAAAGTTGCCACAAACAACTTTGACATATCAAATAAGATTGGAGAAGGTGGTTTTGGTCCTGTTTACAAGGGCCTTCTATCAGATGACACCGCAATTGCTGTTAAGAAGCTTTCTGCCAAATCAAAGCAAGGGAATCGTGAATTTGTGAATGAGATTGGCATGATTTCTGCTCTGCAACACCCTTACCTTGTCAAGCTTTATGGATGTTGTATTGAAGGAAATGACTTGTTGCTTGTCTATGAGTACTTGGAAAATAATAGCGTTGCTCGTGCTCTGTTTGGCCCAAAAGCAAGTCAGGTGAAGATGGATTGGCCAACAAGGCACAGGATCTGTGTTGGTATAGCAAAAGGTTTGGCTTACCTCCATGAGGAATCAAGTTTCGGAATTGTTGTGTTGGAAATAGTCAGTGGGAGGAGTAACACAACTTACCGCTCCGAAGAAGAATGTTTTTATCTTCTTGATTGGGCACTTATTCTAAAGGAGAGAGGGAGTTTGTTGGACCTAGTGGATCCAAGGCTAGGCTCAGAGTTTAACAAAGAACAGATGATTACTACAATCAATGTGGCTCTCCTTTGCTCTCATGTGTCTTCAGCAGTTAGGCCTTCCATGTCTTCAGTGGTGAAAATGCTTGAAGGCAGGGCTTCGGTTCAGGAGGTGATCTCTGATCCAAATGCCTCAAACAATGAAATCAATGCAATGAGGAAACATTTTCAATCCAGTATTGAAGAAAACAATGGTGACCTTGAGAGTCAGAGACAAACTATGTCAATTGAACCATCGTGGACTGCTTCATCTACATCTGCTCATGATCTCTATTCTGTCCATCCTTATTCCAATTATTGGGAGAACAGAAAATAG

Protein Analysis

954

Amino Acids

104.48

Weight (kDa)

5.26

Isoelectric Point (pI)

29.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 216 - 318 6.1e-06 Leucine-rich repeat region
LRR_8 PF13855 275 - 334 1.6e-08 Leucine rich repeat
Malectin PF11721 403 - 594 4.6e-38 Malectin domain
Pkinase PF00069 677 - 793 1.6e-17 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 678 - 793 3.4e-20 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000292)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53420 AT3G14840
fragaria_vesca FvH4_3g17840 FvH4_3g17840 FvH4_3g17840 FvH4_3g17840 FvH4_3g17840 FvH4_3g17840 FvH4_3g17841 FvH4_3g17841 FvH4_3g17841 FvH4_3g17841 FvH4_3g17841 FvH4_3g17841 FvH4_3g17850 FvH4_3g17850 FvH4_3g17850 FvH4_3g17850 FvH4_3g17851
malus_domestica MD03G1246600.v1.1 MD06G1045200.v1.1 MD06G1045400.v1.1 MD11G1267800.v1.1 MD11G1267900.v1.1 MD11G1268100.v1.1 MD11G1268300.v1.1 MD11G1268500.v1.1 MD11G1268600.v1.1
prunus_persica Prupe.4G157700_v2.0.a1 Prupe.4G157700_v2.0.a1 Prupe.4G157800_v2.0.a1 Prupe.4G157800_v2.0.a1 Prupe.4G157800_v2.0.a1 Prupe.4G157800_v2.0.a1 Prupe.4G157800_v2.0.a1 Prupe.4G157900_v2.0.a1 Prupe.4G157900_v2.0.a1 Prupe.4G157900_v2.0.a1
pyrus_communis pycom03g19540 pycom11g23600 pycom11g23630 pycom11g23690 pycom11g23720 pycom11g23730 pycom11g23740 pycom11g23750
rosa_chinensis RchiOBHm_Chr0c40g0503411 RchiOBHm_Chr0c40g0503421 RchiOBHm_Chr5g0029631 RchiOBHm_Chr5g0029661 RchiOBHm_Chr5g0029701 RchiOBHm_Chr5g0029741 RchiOBHm_Chr5g0029771 RchiOBHm_Chr5g0029781 RchiOBHm_Chr5g0029811 RchiOBHm_Chr5g0029851 RchiOBHm_Chr5g0029871 RchiOBHm_Chr5g0029891 RchiOBHm_Chr7g0222221
rosa_laevigata RLG00000017380 RLG00000032873 RLG00000033188 RLG00000033190 RLG00000033192 RLG00000033193 RLG00000033194 RLG00000033196 RLG00000033199 RLG00000033238
rosa_multiflora Rmu_co8359987.1_g000001 Rmu_co8444909.1_g000001 Rmu_sc0001608.1_g000007 Rmu_sc0001608.1_g000009 Rmu_sc0001608.1_g000013 Rmu_sc0001608.1_g000018 Rmu_sc0001608.1_g000023 Rmu_sc0001608.1_g000040 Rmu_sc0003238.1_g000016 Rmu_sc0003368.1_g000026 Rmu_sc0003368.1_g000034 Rmu_sc0003368.1_g000043 Rmu_sc0003368.1_g000047 Rmu_sc0003368.1_g000052 Rmu_sc0003368.1_g000054 Rmu_sc0004963.1_g000001
rosa_roxburghii Rroxscaffold_1G00050080 Rroxscaffold_1G00050090 Rroxscaffold_1G00050110 Rroxscaffold_1G00050120 Rroxscaffold_1G00050180 Rroxscaffold_1G00050190 Rroxscaffold_1G00050220 Rroxscaffold_1G00050270 Rroxscaffold_1G00050300
rosa_rugosa Rorug04G0065200 Rorug05G0114900 Rorug05G0115000 Rorug05G0115100 Rorug05G0120100 Rorug05G0238900
rosa_samantha Rh5AG207800 Rh5AG208000 Rh5AG208300 Rh5AG208400 Rh5AG208800 Rh5AG294900 Rh5AG295000 Rh5AG295400 Rh5CG230400 Rh7AG347800
rosa_wichuraiana Rw0G005370 Rw0G012460 Rw0G019060 Rw5G018910 Rw5G018920 Rw5G018930 Rw5G018940 Rw5G018960 Rw5G018970 Rw5G018980 Rw5G018990 Rw5G019000 Rw7G029840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 236, 1628, 1841
Acc36I ACCTGC 5 cut(s) 236, 1628, 1682, 1841, 1983
Acc65I GGTACC 1 cut(s) 38
AccB1I GGYRCC 2 cut(s) 38, 389
AccB7I CCANNNNNTGG 3 cut(s) 1118, 1829, 2847
AccBSI CCGCTC 1 cut(s) 2439
AccI GTMKAC 1 cut(s) 893
AciI CCGC 9 cut(s) 174, 200, 231, 373, 542, 844, 1839, 2097, 2437
AclWI GGATC 7 cut(s) 158, 793, 1699, 2342, 2510, 2523, 2666
AcoI YGGCCR 3 cut(s) 205, 261, 2318
AcsI RAATTY 7 cut(s) 151, 553, 903, 1076, 1252, 1908, 2147
AcuI CTGAAG 4 cut(s) 81, 1128, 2580, 2604
AfaI GTAC 9 cut(s) 40, 193, 244, 807, 1205, 1239, 1439, 1696, 2252
AflIII ACRYGT 2 cut(s) 888, 1573
AhlI ACTAGT 2 cut(s) 421, 691
AjiI CACGTC 1 cut(s) 1574
AjnI CCWGG 4 cut(s) 825, 1394, 1563, 1822
AjuI GAANNNNNNNTTGG 2 cut(s) 2043, 2075
AloI GAACNNNNNNTCC 2 cut(s) 965, 997
Alw21I GWGCWC 1 cut(s) 2281
Alw26I GTCTC 4 cut(s) 824, 1493, 1749, 2755
AlwI GGATC 7 cut(s) 158, 793, 1699, 2342, 2510, 2523, 2666
AlwNI CAGNNNCTG 3 cut(s) 310, 1634, 2337
AoxI GGCC 8 cut(s) 205, 261, 719, 1304, 2077, 2287, 2318, 2606
ApeKI GCWGC 6 cut(s) 171, 197, 865, 1634, 1688, 1836
ApoI RAATTY 7 cut(s) 151, 553, 903, 1076, 1252, 1908, 2147
Asp700I GAANNNNTTC 1 cut(s) 1925
Asp718I GGTACC 1 cut(s) 38
AspLEI GCGC 1 cut(s) 304
AspS9I GGNCC 9 cut(s) 719, 1304, 1763, 1934, 1972, 2063, 2077, 2288, 2506
AsuC2I CCSGG 1 cut(s) 1303
AsuHPI GGTGA 8 cut(s) 217, 361, 478, 1072, 2317, 2638, 2674, 2756
AvaII GGWCC 5 cut(s) 1763, 1934, 1972, 2063, 2506
BaeGI GKGCMC 1 cut(s) 2476
BaeI ACNNNNGTAYC 2 cut(s) 1281, 1314
BalI TGGCCA 2 cut(s) 207, 2320
BamHI GGATCC 1 cut(s) 2515
BanI GGYRCC 2 cut(s) 38, 389
BanII GRGCYC 1 cut(s) 413
BauI CACGAG 2 cut(s) 1113, 2274
BbsI GAAGAC 4 cut(s) 1576, 1876, 2586, 2610
Bbv12I GWGCWC 1 cut(s) 2281
BbvI GCAGC 6 cut(s) 183, 184, 877, 1621, 1675, 1848
BceAI ACGGC 1 cut(s) 276
BcgI CGANNNNNNTGC 2 cut(s) 2123, 2157
BciT130I CCWGG 4 cut(s) 827, 1396, 1565, 1824
BclI TGATCA 1 cut(s) 336
BcnI CCSGG 1 cut(s) 1303
BcoDI GTCTC 4 cut(s) 824, 1493, 1749, 2755
BcuI ACTAGT 2 cut(s) 421, 691
BfmI CTRYAG 3 cut(s) 238, 1635, 1689
BfuAI ACCTGC 5 cut(s) 236, 1628, 1682, 1841, 1983
BglI GCCNNNNNGGC 1 cut(s) 2326
BglII AGATCT 1 cut(s) 270
BisI GCNGC 7 cut(s) 172, 198, 232, 866, 1635, 1689, 1837
BlsI GCNGC 7 cut(s) 173, 199, 233, 867, 1636, 1690, 1838
BmcAI AGTACT 3 cut(s) 193, 244, 2252
Bme1390I CCNGG 5 cut(s) 827, 1303, 1396, 1565, 1824
Bme18I GGWCC 5 cut(s) 1763, 1934, 1972, 2063, 2506
BmgBI CACGTC 1 cut(s) 1574
BmgT120I GGNCC 9 cut(s) 719, 1304, 1763, 1934, 1972, 2063, 2077, 2288, 2506
BmiI GGNNCC 4 cut(s) 40, 391, 412, 2517
BmrFI CCNGG 5 cut(s) 827, 1303, 1396, 1565, 1824
BmrI ACTGGG 1 cut(s) 609
BmsI GCATC 2 cut(s) 1349, 1621
BmuI ACTGGG 1 cut(s) 609
BoxI GACNNNNGTC 1 cut(s) 1761
BpiI GAAGAC 4 cut(s) 1576, 1876, 2586, 2610
BplI GAGNNNNNCTC 2 cut(s) 2651, 2683
BpmI CTGGAG 5 cut(s) 384, 549, 848, 954, 1896
Bpu10I CCTNAGC 1 cut(s) 1322
BpuEI CTTGAG 4 cut(s) 704, 1794, 2017, 2771
BpuMI CCSGG 1 cut(s) 1303
Bsa29I ATCGAT 2 cut(s) 88, 510
BsaBI GATNNNNATC 3 cut(s) 516, 790, 1371
BsaJI CCNNGG 2 cut(s) 1564, 2519
BsaWI WCCGGW 1 cut(s) 1004
BsaXI ACNNNNNCTCC 2 cut(s) 382, 412
Bse1I ACTGG 7 cut(s) 152, 604, 937, 994, 1357, 1902, 2724
Bse3DI GCAATG 1 cut(s) 2709
Bse8I GATNNNNATC 3 cut(s) 516, 790, 1371
BseBI CCWGG 4 cut(s) 827, 1396, 1565, 1824
BseCI ATCGAT 2 cut(s) 88, 510
BseDI CCNNGG 2 cut(s) 1564, 2519
BseGI GGATG 8 cut(s) 191, 208, 393, 794, 1032, 1047, 2216, 2830
BseJI GATNNNNATC 3 cut(s) 516, 790, 1371
BseMI GCAATG 1 cut(s) 2709
BseMII CTCAG 5 cut(s) 38, 755, 1313, 1422, 2544
BseNI ACTGG 7 cut(s) 152, 604, 937, 994, 1357, 1902, 2724
BseRI GAGGAG 3 cut(s) 1767, 1893, 2434
BseSI GKGCMC 1 cut(s) 2476
BseX3I CGGCCG 1 cut(s) 261
BseXI GCAGC 6 cut(s) 183, 184, 877, 1621, 1675, 1848
BseYI CCCAGC 2 cut(s) 1721, 1818
BsgI GTGCAG 1 cut(s) 1875
Bsh1285I CGRYCG 1 cut(s) 264
BshFI GGCC 8 cut(s) 207, 263, 721, 1306, 2079, 2289, 2320, 2608
BshNI GGYRCC 2 cut(s) 38, 389
BshVI ATCGAT 2 cut(s) 88, 510
BsiEI CGRYCG 1 cut(s) 264
BsiHKAI GWGCWC 1 cut(s) 2281
BsiSI CCGG 3 cut(s) 732, 1005, 1302
BslFI GGGAC 3 cut(s) 134, 702, 1744
BsmAI GTCTC 4 cut(s) 824, 1493, 1749, 2755
BsmFI GGGAC 3 cut(s) 134, 702, 1744
BsmI GAATGC 2 cut(s) 579, 1450
BsnI GGCC 8 cut(s) 207, 263, 721, 1306, 2079, 2289, 2320, 2608
Bsp1286I GDGCHC 3 cut(s) 413, 2281, 2476
Bsp1407I TGTACA 1 cut(s) 1437
BspACI CCGC 9 cut(s) 174, 200, 231, 373, 542, 844, 1839, 2097, 2437
BspANI GGCC 8 cut(s) 207, 263, 721, 1306, 2079, 2289, 2320, 2608
BspCNI CTCAG 5 cut(s) 37, 756, 1314, 1423, 2543
BspDI ATCGAT 2 cut(s) 88, 510
BspHI TCATGA 1 cut(s) 2812
BspLI GGNNCC 4 cut(s) 40, 391, 412, 2517
BspMAI CTGCAG 2 cut(s) 1639, 1693
BspMI ACCTGC 5 cut(s) 236, 1628, 1682, 1841, 1983
BspPI GGATC 7 cut(s) 158, 793, 1699, 2342, 2510, 2523, 2666
BspQI GCTCTTC 1 cut(s) 1147
BspT107I GGYRCC 2 cut(s) 38, 389
BsrBI CCGCTC 1 cut(s) 2439
BsrDI GCAATG 1 cut(s) 2709
BsrGI TGTACA 1 cut(s) 1437
BsrI ACTGG 7 cut(s) 152, 604, 937, 994, 1357, 1902, 2724
BssECI CCNNGG 2 cut(s) 1564, 2519
BssSI CACGAG 2 cut(s) 1113, 2274
BssT1I CCWWGG 1 cut(s) 2519
Bst2BI CACGAG 2 cut(s) 1113, 2274
Bst2UI CCWGG 4 cut(s) 827, 1396, 1565, 1824
Bst4CI ACNGT 5 cut(s) 239, 449, 635, 1012, 1510
Bst6I CTCTTC 2 cut(s) 1147, 1215
BstAPI GCANNNNNTGC 1 cut(s) 1634
BstAUI TGTACA 1 cut(s) 1437
BstC8I GCNNGC 3 cut(s) 67, 1138, 1450
BstDEI CTNAG 6 cut(s) 24, 764, 914, 1322, 1431, 2530
BstEII GGTNACC 1 cut(s) 2744
BstENI CCTNNNNNAGG 2 cut(s) 1749, 2372
BstF5I GGATG 8 cut(s) 191, 208, 393, 794, 1032, 1047, 2216, 2830
BstHHI GCGC 1 cut(s) 304
BstMAI GTCTC 4 cut(s) 824, 1493, 1749, 2755
BstMCI CGRYCG 1 cut(s) 264
BstMWI GCNNNNNNNGC 5 cut(s) 71, 80, 1353, 1634, 2326
BstNI CCWGG 4 cut(s) 827, 1396, 1565, 1824
BstNSI RCATGY 2 cut(s) 892, 1000
BstPAI GACNNNNGTC 1 cut(s) 1761
BstPI GGTNACC 1 cut(s) 2744
BstSCI CCNGG 5 cut(s) 825, 1301, 1394, 1563, 1822
BstSFI CTRYAG 3 cut(s) 238, 1635, 1689
BstSLI GKGCMC 1 cut(s) 2476
BstV1I GCAGC 6 cut(s) 183, 184, 877, 1621, 1675, 1848
BstV2I GAAGAC 4 cut(s) 1576, 1876, 2586, 2610
BstX2I RGATCY 6 cut(s) 163, 270, 785, 1704, 2334, 2515
BstXI CCANNNNNNTGG 1 cut(s) 1023
BstYI RGATCY 6 cut(s) 163, 270, 785, 1704, 2334, 2515
BstZI CGGCCG 1 cut(s) 261
Bsu15I ATCGAT 2 cut(s) 88, 510
BsuRI GGCC 8 cut(s) 207, 263, 721, 1306, 2079, 2289, 2320, 2608
BsuTUI ATCGAT 2 cut(s) 88, 510
BtrI CACGTC 1 cut(s) 1574
BtsCI GGATG 8 cut(s) 191, 208, 393, 794, 1032, 1047, 2216, 2830
BtsI GCAGTG 2 cut(s) 1671, 1863
BtsIMutI CAGTG 4 cut(s) 1671, 1863, 2419, 2628
BveI ACCTGC 5 cut(s) 236, 1628, 1682, 1841, 1983
Cac8I GCNNGC 3 cut(s) 67, 1138, 1450
CaiI CAGNNNCTG 3 cut(s) 310, 1634, 2337
CciI TCATGA 1 cut(s) 2812
CfoI GCGC 1 cut(s) 304
Cfr13I GGNCC 9 cut(s) 719, 1304, 1763, 1934, 1972, 2063, 2077, 2288, 2506
ClaI ATCGAT 2 cut(s) 88, 510
Csp6I GTAC 9 cut(s) 39, 192, 243, 806, 1204, 1238, 1438, 1695, 2251
CspCI CAANNNNNGTGG 4 cut(s) 841, 876, 2550, 2585
CviQI GTAC 9 cut(s) 39, 192, 243, 806, 1204, 1238, 1438, 1695, 2251
DdeI CTNAG 6 cut(s) 24, 764, 914, 1322, 1431, 2530
DraI TTTAAA 1 cut(s) 900
EaeI YGGCCR 3 cut(s) 205, 261, 2318
EagI CGGCCG 1 cut(s) 261
Eam1104I CTCTTC 2 cut(s) 1147, 1215
EarI CTCTTC 2 cut(s) 1147, 1215
EclXI CGGCCG 1 cut(s) 261
Eco130I CCWWGG 1 cut(s) 2519
Eco147I AGGCCT 1 cut(s) 2608
Eco24I GRGCYC 1 cut(s) 413
Eco32I GATATC 1 cut(s) 940
Eco47I GGWCC 5 cut(s) 1763, 1934, 1972, 2063, 2506
Eco52I CGGCCG 1 cut(s) 261
Eco57I CTGAAG 4 cut(s) 81, 1128, 2580, 2604
Eco91I GGTNACC 1 cut(s) 2744
EcoNI CCTNNNNNAGG 2 cut(s) 1749, 2372
EcoO109I RGGNCCY 1 cut(s) 2077
EcoO65I GGTNACC 1 cut(s) 2744
EcoRI GAATTC 1 cut(s) 1908
EcoRII CCWGG 4 cut(s) 825, 1394, 1563, 1822
EcoRV GATATC 1 cut(s) 940
EcoT14I CCWWGG 1 cut(s) 2519
EcoT38I GRGCYC 1 cut(s) 413
ErhI CCWWGG 1 cut(s) 2519
FaqI GGGAC 3 cut(s) 134, 702, 1744
FauI CCCGC 1 cut(s) 380
FbaI TGATCA 1 cut(s) 336
FblI GTMKAC 1 cut(s) 893
Fnu4HI GCNGC 7 cut(s) 172, 198, 232, 866, 1635, 1689, 1837
FokI GGATG 8 cut(s) 198, 215, 380, 781, 1039, 1054, 2223, 2817
FriOI GRGCYC 1 cut(s) 413
Fsp4HI GCNGC 7 cut(s) 172, 198, 232, 866, 1635, 1689, 1837
GlaI GCGC 1 cut(s) 303
GluI GCNGC 7 cut(s) 172, 198, 232, 866, 1635, 1689, 1837
GsaI CCCAGC 2 cut(s) 1725, 1822
GsuI CTGGAG 5 cut(s) 384, 549, 848, 954, 1896
HaeIII GGCC 8 cut(s) 207, 263, 721, 1306, 2079, 2289, 2320, 2608
HapII CCGG 3 cut(s) 732, 1005, 1302
HhaI GCGC 1 cut(s) 304
Hin6I GCGC 1 cut(s) 302
HinP1I GCGC 1 cut(s) 302
HincII GTYRAC 1 cut(s) 894
HindII GTYRAC 1 cut(s) 894
HindIII AAGCTT 3 cut(s) 111, 2114, 2201
HpaII CCGG 3 cut(s) 732, 1005, 1302
HphI GGTGA 8 cut(s) 217, 361, 478, 1072, 2317, 2638, 2674, 2756
HpyAV CCTTC 9 cut(s) 322, 738, 1317, 1957, 2045, 2090, 2216, 2619, 2633
HpyCH4III ACNGT 5 cut(s) 239, 449, 635, 1012, 1510
HpyCH4IV ACGT 3 cut(s) 222, 1573, 1680
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 80, 1353, 1634, 2326
HpyF3I CTNAG 6 cut(s) 24, 764, 914, 1322, 1431, 2530
HpySE526I ACGT 3 cut(s) 222, 1573, 1680
HspAI GCGC 1 cut(s) 302
KpnI GGTACC 1 cut(s) 42
Ksp22I TGATCA 1 cut(s) 336
LguI GCTCTTC 1 cut(s) 1147
LmnI GCTCC 5 cut(s) 80, 331, 416, 544, 2444
Lsp1109I GCAGC 6 cut(s) 183, 184, 877, 1621, 1675, 1848
LweI GCATC 2 cut(s) 1349, 1621
MaeII ACGT 3 cut(s) 222, 1573, 1680
MaeIII GTNAC 8 cut(s) 223, 280, 629, 1599, 1657, 1681, 2423, 2744
MbiI CCGCTC 1 cut(s) 2439
MfeI CAATTG 2 cut(s) 2100, 2775
MflI RGATCY 6 cut(s) 163, 270, 785, 1704, 2334, 2515
MhlI GDGCHC 3 cut(s) 413, 2281, 2476
MlsI TGGCCA 2 cut(s) 207, 2320
MluNI TGGCCA 2 cut(s) 207, 2320
MlyI GAGTC 3 cut(s) 1407, 1890, 2764
MmeI TCCRAC 4 cut(s) 421, 1843, 2382, 2484
Mox20I TGGCCA 2 cut(s) 207, 2320
MroXI GAANNNNTTC 1 cut(s) 1925
MscI TGGCCA 2 cut(s) 207, 2320
MseI TTAA 7 cut(s) 116, 755, 899, 921, 986, 2109, 2538
Msp20I TGGCCA 2 cut(s) 207, 2320
MspA1I CMGCKG 3 cut(s) 174, 1034, 1839
MspI CCGG 3 cut(s) 732, 1005, 1302
MspR9I CCNGG 5 cut(s) 827, 1303, 1396, 1565, 1824
MunI CAATTG 2 cut(s) 2100, 2775
Mva1269I GAATGC 2 cut(s) 579, 1450
MvaI CCWGG 4 cut(s) 827, 1396, 1565, 1824
MwoI GCNNNNNNNGC 5 cut(s) 71, 80, 1353, 1634, 2326
NciI CCSGG 1 cut(s) 1303
NlaIV GGNNCC 4 cut(s) 40, 391, 412, 2517
NmuCI GTSAC 6 cut(s) 223, 280, 629, 1599, 1657, 2744
NspI RCATGY 2 cut(s) 892, 1000
PagI TCATGA 1 cut(s) 2812
PaqCI CACCTGC 3 cut(s) 236, 1628, 1841
PceI AGGCCT 1 cut(s) 2608
PciI ACATGT 1 cut(s) 888
PciSI GCTCTTC 1 cut(s) 1147
PcsI WCGNNNNNNNCGW 1 cut(s) 1278
PctI GAATGC 2 cut(s) 579, 1450
PdmI GAANNNNTTC 1 cut(s) 1925
PfeI GAWTC 8 cut(s) 307, 697, 791, 1426, 1790, 1866, 2140, 2378
PflMI CCANNNNNTGG 3 cut(s) 1118, 1829, 2847
PkrI GCNGC 7 cut(s) 173, 199, 233, 867, 1636, 1690, 1838
PleI GAGTC 3 cut(s) 1407, 1889, 2763
PpsI GAGTC 3 cut(s) 1407, 1889, 2763
PscI ACATGT 1 cut(s) 888
PshAI GACNNNNGTC 1 cut(s) 1761
Psp6I CCWGG 4 cut(s) 825, 1394, 1563, 1822
PspEI GGTNACC 1 cut(s) 2744
PspFI CCCAGC 2 cut(s) 1721, 1818
PspGI CCWGG 4 cut(s) 825, 1394, 1563, 1822
PspN4I GGNNCC 4 cut(s) 40, 391, 412, 2517
PspPI GGNCC 9 cut(s) 719, 1304, 1763, 1934, 1972, 2063, 2077, 2288, 2506
PstI CTGCAG 2 cut(s) 1639, 1693
PstNI CAGNNNCTG 3 cut(s) 310, 1634, 2337
PsuI RGATCY 6 cut(s) 163, 270, 785, 1704, 2334, 2515
PvuII CAGCTG 1 cut(s) 1034
RsaI GTAC 9 cut(s) 40, 193, 244, 807, 1205, 1239, 1439, 1696, 2252
RsaNI GTAC 9 cut(s) 39, 192, 243, 806, 1204, 1238, 1438, 1695, 2251
SalI GTCGAC 1 cut(s) 892
SapI GCTCTTC 1 cut(s) 1147
SaqAI TTAA 7 cut(s) 116, 755, 899, 921, 986, 2109, 2538
SatI GCNGC 7 cut(s) 172, 198, 232, 866, 1635, 1689, 1837
Sau96I GGNCC 9 cut(s) 719, 1304, 1763, 1934, 1972, 2063, 2077, 2288, 2506
ScaI AGTACT 3 cut(s) 193, 244, 2252
SchI GAGTC 3 cut(s) 1407, 1890, 2764
ScrFI CCNGG 5 cut(s) 827, 1303, 1396, 1565, 1824
SduI GDGCHC 3 cut(s) 413, 2281, 2476
SfaNI GCATC 2 cut(s) 1349, 1621
SfcI CTRYAG 3 cut(s) 238, 1635, 1689
SinI GGWCC 5 cut(s) 1763, 1934, 1972, 2063, 2506
SmlI CTYRAG 4 cut(s) 683, 1809, 1996, 2750
SmoI CTYRAG 4 cut(s) 683, 1809, 1996, 2750
SpeI ACTAGT 2 cut(s) 421, 691
SseBI AGGCCT 1 cut(s) 2608
SsiI CCGC 9 cut(s) 174, 200, 231, 373, 542, 844, 1839, 2097, 2437
SspI AATATT 3 cut(s) 856, 1536, 1624
StuI AGGCCT 1 cut(s) 2608
StyD4I CCNGG 5 cut(s) 825, 1301, 1394, 1563, 1822
StyI CCWWGG 1 cut(s) 2519
TaaI ACNGT 5 cut(s) 239, 449, 635, 1012, 1510
TaiI ACGT 3 cut(s) 225, 1576, 1683
TaqI TCGA 5 cut(s) 88, 510, 587, 893, 1581
TatI WGTACW 5 cut(s) 191, 242, 805, 1437, 2250
TauI GCSGC 1 cut(s) 234
TfiI GAWTC 8 cut(s) 307, 697, 791, 1426, 1790, 1866, 2140, 2378
Tru1I TTAA 7 cut(s) 116, 755, 899, 921, 986, 2109, 2538
Tru9I TTAA 7 cut(s) 116, 755, 899, 921, 986, 2109, 2538
TscAI CASTG 4 cut(s) 1678, 1863, 2419, 2628
TseFI GTSAC 6 cut(s) 223, 280, 629, 1599, 1657, 2744
TseI GCWGC 6 cut(s) 171, 197, 865, 1634, 1688, 1836
Tsp45I GTSAC 6 cut(s) 223, 280, 629, 1599, 1657, 2744
TspGWI ACGGA 1 cut(s) 1697
TspRI CASTG 4 cut(s) 1678, 1863, 2419, 2628
Van91I CCANNNNNTGG 3 cut(s) 1118, 1829, 2847
VpaK11BI GGWCC 5 cut(s) 1763, 1934, 1972, 2063, 2506
XagI CCTNNNNNAGG 2 cut(s) 1749, 2372
XapI RAATTY 7 cut(s) 151, 553, 903, 1076, 1252, 1908, 2147
XceI RCATGY 2 cut(s) 892, 1000
XcmI CCANNNNNNNNNTGG 2 cut(s) 1826, 2620
XmiI GTMKAC 1 cut(s) 893
XmnI GAANNNNTTC 1 cut(s) 1925
ZrmI AGTACT 3 cut(s) 193, 244, 2252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.