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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 299 |
| AcsI | RAATTY | 2 cut(s) | 38, 141 |
| AgsI | TTSAA | 2 cut(s) | 136, 173 |
| AluBI | AGCT | 1 cut(s) | 305 |
| AluI | AGCT | 1 cut(s) | 305 |
| Alw21I | GWGCWC | 1 cut(s) | 243 |
| Alw26I | GTCTC | 1 cut(s) | 336 |
| AlwI | GGATC | 1 cut(s) | 299 |
| ApoI | RAATTY | 2 cut(s) | 38, 141 |
| AsuHPI | GGTGA | 1 cut(s) | 176 |
| BaeGI | GKGCMC | 1 cut(s) | 255 |
| Bbv12I | GWGCWC | 1 cut(s) | 243 |
| BccI | CCATC | 4 cut(s) | 86, 174, 263, 292 |
| BceAI | ACGGC | 1 cut(s) | 138 |
| BcoDI | GTCTC | 1 cut(s) | 336 |
| BfaI | CTAG | 1 cut(s) | 306 |
| BmsI | GCATC | 1 cut(s) | 335 |
| BoxI | GACNNNNGTC | 1 cut(s) | 384 |
| BpmI | CTGGAG | 1 cut(s) | 306 |
| BsaJI | CCNNGG | 1 cut(s) | 287 |
| Bse118I | RCCGGY | 1 cut(s) | 155 |
| BseDI | CCNNGG | 1 cut(s) | 287 |
| BseGI | GGATG | 1 cut(s) | 193 |
| BseRI | GAGGAG | 1 cut(s) | 35 |
| BseSI | GKGCMC | 1 cut(s) | 255 |
| BsiHKAI | GWGCWC | 1 cut(s) | 243 |
| BsiSI | CCGG | 3 cut(s) | 156, 209, 246 |
| BsmAI | GTCTC | 1 cut(s) | 336 |
| BsmBI | CGTCTC | 1 cut(s) | 336 |
| Bsp1286I | GDGCHC | 2 cut(s) | 243, 255 |
| Bsp143I | GATC | 3 cut(s) | 66, 166, 291 |
| BspPI | GGATC | 1 cut(s) | 299 |
| BsrFI | RCCGGY | 1 cut(s) | 155 |
| BssAI | RCCGGY | 1 cut(s) | 155 |
| BssECI | CCNNGG | 1 cut(s) | 287 |
| BssMI | GATC | 3 cut(s) | 66, 166, 291 |
| BssT1I | CCWWGG | 1 cut(s) | 287 |
| Bst4CI | ACNGT | 1 cut(s) | 164 |
| BstC8I | GCNNGC | 1 cut(s) | 251 |
| BstF5I | GGATG | 1 cut(s) | 193 |
| BstKTI | GATC | 3 cut(s) | 69, 169, 294 |
| BstMAI | GTCTC | 1 cut(s) | 336 |
| BstMBI | GATC | 3 cut(s) | 66, 166, 291 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 384 |
| BstSLI | GKGCMC | 1 cut(s) | 255 |
| BtsCI | GGATG | 1 cut(s) | 193 |
| Cac8I | GCNNGC | 1 cut(s) | 251 |
| Cfr10I | RCCGGY | 1 cut(s) | 155 |
| CviJI | RGCY | 4 cut(s) | 212, 305, 316, 356 |
| CviKI_1 | RGCY | 4 cut(s) | 212, 305, 316, 356 |
| DpnI | GATC | 3 cut(s) | 68, 168, 293 |
| DpnII | GATC | 3 cut(s) | 66, 166, 291 |
| Eco130I | CCWWGG | 1 cut(s) | 287 |
| EcoRI | GAATTC | 1 cut(s) | 38 |
| EcoT14I | CCWWGG | 1 cut(s) | 287 |
| ErhI | CCWWGG | 1 cut(s) | 287 |
| Esp3I | CGTCTC | 1 cut(s) | 336 |
| FaiI | YATR | 4 cut(s) | 48, 190, 270, 272 |
| FokI | GGATG | 1 cut(s) | 180 |
| FspBI | CTAG | 1 cut(s) | 306 |
| GsuI | CTGGAG | 1 cut(s) | 306 |
| HapII | CCGG | 3 cut(s) | 156, 209, 246 |
| HincII | GTYRAC | 1 cut(s) | 84 |
| HindII | GTYRAC | 1 cut(s) | 84 |
| HinfI | GANTC | 1 cut(s) | 319 |
| HpaII | CCGG | 3 cut(s) | 156, 209, 246 |
| HphI | GGTGA | 1 cut(s) | 176 |
| Hpy166II | GTNNAC | 2 cut(s) | 84, 115 |
| Hpy188I | TCNGA | 2 cut(s) | 59, 153 |
| Hpy188III | TCNNGA | 1 cut(s) | 295 |
| Hpy8I | GTNNAC | 2 cut(s) | 84, 115 |
| Hpy99I | CGWCG | 2 cut(s) | 124, 130 |
| HpyAV | CCTTC | 3 cut(s) | 142, 208, 220 |
| HpyCH4III | ACNGT | 1 cut(s) | 164 |
| HpyCH4V | TGCA | 1 cut(s) | 107 |
| Kzo9I | GATC | 3 cut(s) | 66, 166, 291 |
| LmnI | GCTCC | 1 cut(s) | 289 |
| LpnPI | CCDG | 6 cut(s) | 169, 198, 222, 259, 335, 336 |
| LweI | GCATC | 1 cut(s) | 335 |
| MaeI | CTAG | 1 cut(s) | 306 |
| MalI | GATC | 3 cut(s) | 68, 168, 293 |
| MboI | GATC | 3 cut(s) | 66, 166, 291 |
| MboII | GAAGA | 2 cut(s) | 161, 271 |
| MhlI | GDGCHC | 2 cut(s) | 243, 255 |
| MluCI | AATT | 3 cut(s) | 38, 141, 363 |
| MlyI | GAGTC | 1 cut(s) | 313 |
| MnlI | CCTC | 3 cut(s) | 13, 16, 377 |
| MslI | CAYNNNNRTG | 1 cut(s) | 58 |
| MspI | CCGG | 3 cut(s) | 156, 209, 246 |
| NdeII | GATC | 3 cut(s) | 66, 166, 291 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 125 |
| PleI | GAGTC | 1 cut(s) | 313 |
| PpsI | GAGTC | 1 cut(s) | 313 |
| PshAI | GACNNNNGTC | 1 cut(s) | 384 |
| RseI | CAYNNNNRTG | 1 cut(s) | 58 |
| Sau3AI | GATC | 3 cut(s) | 66, 166, 291 |
| SchI | GAGTC | 1 cut(s) | 313 |
| SduI | GDGCHC | 2 cut(s) | 243, 255 |
| SetI | ASST | 4 cut(s) | 134, 231, 307, 388 |
| SfaNI | GCATC | 1 cut(s) | 335 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 58 |
| Sse9I | AATT | 3 cut(s) | 38, 141, 363 |
| SspI | AATATT | 1 cut(s) | 33 |
| SspMI | CTAG | 1 cut(s) | 306 |
| StyI | CCWWGG | 1 cut(s) | 287 |
| TaaI | ACNGT | 1 cut(s) | 164 |
| TaqI | TCGA | 1 cut(s) | 119 |
| TasI | AATT | 3 cut(s) | 38, 141, 363 |
| TspDTI | ATGAA | 1 cut(s) | 113 |
| XapI | RAATTY | 2 cut(s) | 38, 141 |
| XspI | CTAG | 1 cut(s) | 306 |