Rroxscaffold_4G00282260

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000783
Physical Location & Seq
Forward (+)
4324184 .. 4326539
2356 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00282260.1

Sequence Viewer

Length: 717 bp
ATGCCGTTGTCATTGATGCTCAACCAAGTCTTTGTCATCCCTTGTTTAATGCTTCTCACTTACCGCCGCTCTTTCCTCCCTGGTCGAAGCACACATCTAGCCTCAAAGCTTGCAGATTTATCACTAAGGGTTTATGCTCAACTCAATACTTGTCCCCGTGTGCCACTTGATGTCGTGGCCCTCCCATCAAGCCGAGGAGGCGGTGATAATTCTCTTCTTTTACCTCTCTCGTTGGTTAGAAGAGGCGGTTTTGTCGATGTTGGTGGGTTGGGGATAGAAACCCTAGTTGACCGGATCTGGTTTGGTGTTCTTATTGGTAGAGATGATGGATCTACTCTTGATCCGTTGACCTATGGTGGCAAGCGGCGAGTGATTGGAGTACCGCCGTGGCGGGGCGGCGTTCGACCCTATGGGGAATGCGGGCGAGGCAAGGGCTTCGCCTTCTTCTCCATTTCCTTGCGGTCTTCCAACGGCTATAGTGGCACGGCTTTCGGGGACGGAGCAACCGGTATGAGTCTTGGGTTAATGAGGACGGAGTCTCGGGTCTTCGATGGTGGAGGCGATTTTTGTTTTGGGTTGCGGCATAGGGGCCGTGGCCGGTGGCCATCGGTGGCGCTTGGTACGGAGATGGAGGATGCACGAGAGGTCGGCGGTGGTGGCGACAACTATGAGGAAGGCGAAGACCGCGACAAAACTTGGGCGGCGGGCGACGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

238

Amino Acids

25.58

Weight (kDa)

8.76

Isoelectric Point (pI)

45.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 69
AccII CGCG 1 cut(s) 687
AclWI GGATC 3 cut(s) 302, 335, 337
AcoI YGGCCR 2 cut(s) 595, 602
AfaI GTAC 2 cut(s) 381, 622
AfiI CCNNNNNNNGG 1 cut(s) 392
AflIII ACRYGT 1 cut(s) 711
AgeI ACCGGT 1 cut(s) 506
AjiI CACGTC 1 cut(s) 712
AjnI CCWGG 1 cut(s) 79
AluBI AGCT 1 cut(s) 109
AluI AGCT 1 cut(s) 109
Alw26I GTCTC 1 cut(s) 543
AlwI GGATC 3 cut(s) 302, 335, 337
Ama87I CYCGRG 1 cut(s) 540
AoxI GGCC 4 cut(s) 177, 589, 595, 602
AsiGI ACCGGT 1 cut(s) 506
AspLEI GCGC 1 cut(s) 616
AspS9I GGNCC 2 cut(s) 178, 589
AsuHPI GGTGA 1 cut(s) 215
AvaI CYCGRG 1 cut(s) 540
BalI TGGCCA 1 cut(s) 604
BauI CACGAG 1 cut(s) 639
BbsI GAAGAC 3 cut(s) 456, 538, 687
BccI CCATC 5 cut(s) 193, 320, 545, 613, 622
BceAI ACGGC 4 cut(s) 370, 487, 501, 576
BcgI CGANNNNNNTGC 4 cut(s) 418, 452, 472, 506
BciT130I CCWGG 1 cut(s) 81
BcoDI GTCTC 1 cut(s) 543
BfaI CTAG 2 cut(s) 98, 284
BfmI CTRYAG 1 cut(s) 475
BfoI RGCGCY 1 cut(s) 617
BglI GCCNNNNNGGC 1 cut(s) 198
BisI GCNGC 5 cut(s) 67, 365, 397, 581, 702
BlsI GCNGC 5 cut(s) 68, 366, 398, 582, 703
Bme1390I CCNGG 1 cut(s) 81
BmeT110I CYCGRG 1 cut(s) 540
BmgBI CACGTC 1 cut(s) 712
BmgT120I GGNCC 2 cut(s) 178, 589
BmiI GGNNCC 1 cut(s) 590
BmrFI CCNGG 1 cut(s) 81
BmsI GCATC 2 cut(s) 6, 625
BpiI GAAGAC 3 cut(s) 456, 538, 687
BsaJI CCNNGG 4 cut(s) 79, 193, 386, 592
BsaWI WCCGGW 2 cut(s) 291, 506
Bsc4I CCNNNNNNNGG 1 cut(s) 392
Bse118I RCCGGY 2 cut(s) 506, 597
BseBI CCWGG 1 cut(s) 81
BseDI CCNNGG 4 cut(s) 79, 193, 386, 592
BseGI GGATG 2 cut(s) 36, 640
BseLI CCNNNNNNNGG 1 cut(s) 392
BseRI GAGGAG 1 cut(s) 210
Bsh1236I CGCG 1 cut(s) 687
BshFI GGCC 4 cut(s) 179, 591, 597, 604
BshTI ACCGGT 1 cut(s) 506
BsiHKCI CYCGRG 1 cut(s) 540
BsiSI CCGG 3 cut(s) 292, 507, 598
BslFI GGGAC 2 cut(s) 138, 509
BslI CCNNNNNNNGG 1 cut(s) 392
BsmAI GTCTC 1 cut(s) 543
BsmFI GGGAC 2 cut(s) 138, 509
BsmI GAATGC 1 cut(s) 422
BsnI GGCC 4 cut(s) 179, 591, 597, 604
BsoBI CYCGRG 1 cut(s) 540
Bsp143I GATC 3 cut(s) 294, 329, 340
BspANI GGCC 4 cut(s) 179, 591, 597, 604
BspFNI CGCG 1 cut(s) 687
BspLI GGNNCC 1 cut(s) 590
BspPI GGATC 3 cut(s) 302, 335, 337
BsrBI CCGCTC 1 cut(s) 69
BsrFI RCCGGY 2 cut(s) 506, 597
BssAI RCCGGY 2 cut(s) 506, 597
BssECI CCNNGG 4 cut(s) 79, 193, 386, 592
BssMI GATC 3 cut(s) 294, 329, 340
BssSI CACGAG 1 cut(s) 639
Bst2BI CACGAG 1 cut(s) 639
Bst2UI CCWGG 1 cut(s) 81
Bst6I CTCTTC 2 cut(s) 219, 235
BstC8I GCNNGC 4 cut(s) 111, 362, 422, 706
BstDEI CTNAG 1 cut(s) 125
BstDSI CCRYGG 2 cut(s) 386, 592
BstF5I GGATG 2 cut(s) 36, 640
BstFNI CGCG 1 cut(s) 687
BstH2I RGCGCY 1 cut(s) 617
BstHHI GCGC 1 cut(s) 616
BstKTI GATC 3 cut(s) 297, 332, 343
BstMAI GTCTC 1 cut(s) 543
BstMBI GATC 3 cut(s) 294, 329, 340
BstMWI GCNNNNNNNGC 5 cut(s) 198, 426, 480, 657, 684
BstNI CCWGG 1 cut(s) 81
BstSCI CCNGG 1 cut(s) 79
BstSFI CTRYAG 1 cut(s) 475
BstUI CGCG 1 cut(s) 687
BstV2I GAAGAC 3 cut(s) 456, 538, 687
BstX2I RGATCY 2 cut(s) 294, 329
BstYI RGATCY 2 cut(s) 294, 329
BsuRI GGCC 4 cut(s) 179, 591, 597, 604
BtgI CCRYGG 2 cut(s) 386, 592
BtrI CACGTC 1 cut(s) 712
BtsCI GGATG 2 cut(s) 36, 640
Cac8I GCNNGC 4 cut(s) 111, 362, 422, 706
CfoI GCGC 1 cut(s) 616
Cfr10I RCCGGY 2 cut(s) 506, 597
Cfr13I GGNCC 2 cut(s) 178, 589
Csp6I GTAC 2 cut(s) 380, 621
CspAI ACCGGT 1 cut(s) 506
CviQI GTAC 2 cut(s) 380, 621
DdeI CTNAG 1 cut(s) 125
DpnI GATC 3 cut(s) 296, 331, 342
DpnII GATC 3 cut(s) 294, 329, 340
EaeI YGGCCR 2 cut(s) 595, 602
Eam1104I CTCTTC 2 cut(s) 219, 235
EarI CTCTTC 2 cut(s) 219, 235
Eco88I CYCGRG 1 cut(s) 540
EcoRII CCWGG 1 cut(s) 79
FaiI YATR 7 cut(s) 135, 354, 411, 477, 512, 585, 669
FaqI GGGAC 2 cut(s) 138, 509
FauI CCCGC 3 cut(s) 384, 413, 697
Fnu4HI GCNGC 5 cut(s) 67, 365, 397, 581, 702
FokI GGATG 2 cut(s) 23, 647
Fsp4HI GCNGC 5 cut(s) 67, 365, 397, 581, 702
FspBI CTAG 2 cut(s) 98, 284
GlaI GCGC 1 cut(s) 615
GluI GCNGC 5 cut(s) 67, 365, 397, 581, 702
HaeII RGCGCY 1 cut(s) 617
HaeIII GGCC 4 cut(s) 179, 591, 597, 604
HapII CCGG 3 cut(s) 292, 507, 598
HhaI GCGC 1 cut(s) 616
Hin6I GCGC 1 cut(s) 614
HinP1I GCGC 1 cut(s) 614
HincII GTYRAC 2 cut(s) 289, 348
HindII GTYRAC 2 cut(s) 289, 348
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 2 cut(s) 514, 536
HpaII CCGG 3 cut(s) 292, 507, 598
HphI GGTGA 1 cut(s) 215
Hpy166II GTNNAC 2 cut(s) 289, 348
Hpy188III TCNNGA 1 cut(s) 338
Hpy8I GTNNAC 2 cut(s) 289, 348
Hpy99I CGWCG 1 cut(s) 713
HpyAV CCTTC 2 cut(s) 451, 668
HpyCH4IV ACGT 1 cut(s) 711
HpyCH4V TGCA 2 cut(s) 113, 638
HpyF10VI GCNNNNNNNGC 5 cut(s) 198, 426, 480, 657, 684
HpyF3I CTNAG 1 cut(s) 125
HpySE526I ACGT 1 cut(s) 711
HspAI GCGC 1 cut(s) 614
Kzo9I GATC 3 cut(s) 294, 329, 340
LmnI GCTCC 1 cut(s) 500
LpnPI CCDG 6 cut(s) 66, 93, 283, 305, 520, 611
LweI GCATC 2 cut(s) 6, 625
MaeI CTAG 2 cut(s) 98, 284
MaeII ACGT 1 cut(s) 711
MalI GATC 3 cut(s) 296, 331, 342
MbiI CCGCTC 1 cut(s) 69
MboI GATC 3 cut(s) 294, 329, 340
MboII GAAGA 6 cut(s) 206, 252, 436, 456, 538, 692
MflI RGATCY 2 cut(s) 294, 329
MlsI TGGCCA 1 cut(s) 604
MluCI AATT 1 cut(s) 208
MluNI TGGCCA 1 cut(s) 604
MlyI GAGTC 2 cut(s) 523, 545
MmeI TCCRAC 1 cut(s) 492
Mox20I TGGCCA 1 cut(s) 604
MscI TGGCCA 1 cut(s) 604
MseI TTAA 2 cut(s) 47, 524
Msp20I TGGCCA 1 cut(s) 604
MspI CCGG 3 cut(s) 292, 507, 598
MspR9I CCNGG 1 cut(s) 81
Mva1269I GAATGC 1 cut(s) 422
MvaI CCWGG 1 cut(s) 81
MvnI CGCG 1 cut(s) 687
MwoI GCNNNNNNNGC 5 cut(s) 198, 426, 480, 657, 684
NdeII GATC 3 cut(s) 294, 329, 340
NlaIV GGNNCC 1 cut(s) 590
NmeAIII GCCGAG 1 cut(s) 218
PctI GAATGC 1 cut(s) 422
PflFI GACNNNGTC 1 cut(s) 535
PinAI ACCGGT 1 cut(s) 506
PkrI GCNGC 5 cut(s) 68, 366, 398, 582, 703
PleI GAGTC 2 cut(s) 522, 544
PpsI GAGTC 2 cut(s) 522, 544
Psp6I CCWGG 1 cut(s) 79
PspGI CCWGG 1 cut(s) 79
PspN4I GGNNCC 1 cut(s) 590
PspPI GGNCC 2 cut(s) 178, 589
PsuI RGATCY 2 cut(s) 294, 329
PsyI GACNNNGTC 1 cut(s) 535
RsaI GTAC 2 cut(s) 381, 622
RsaNI GTAC 2 cut(s) 380, 621
SaqAI TTAA 2 cut(s) 47, 524
SatI GCNGC 5 cut(s) 67, 365, 397, 581, 702
Sau3AI GATC 3 cut(s) 294, 329, 340
Sau96I GGNCC 2 cut(s) 178, 589
SchI GAGTC 2 cut(s) 523, 545
ScrFI CCNGG 1 cut(s) 81
SetI ASST 5 cut(s) 111, 226, 353, 648, 714
SfaNI GCATC 2 cut(s) 6, 625
SfcI CTRYAG 1 cut(s) 475
Sse9I AATT 1 cut(s) 208
SspMI CTAG 2 cut(s) 98, 284
StyD4I CCNGG 1 cut(s) 79
TaiI ACGT 1 cut(s) 714
TaqI TCGA 4 cut(s) 85, 255, 403, 549
TasI AATT 1 cut(s) 208
TauI GCSGC 5 cut(s) 69, 367, 399, 583, 704
Tru1I TTAA 2 cut(s) 47, 524
Tru9I TTAA 2 cut(s) 47, 524
TspGWI ACGGA 4 cut(s) 333, 513, 548, 638
Tth111I GACNNNGTC 1 cut(s) 535
XspI CTAG 2 cut(s) 98, 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.