Rorug03G0193800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000783
Physical Location & Seq
Reverse (-)
17024419 .. 17026333
1915 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0193800.1

Sequence Viewer

Length: 1290 bp
ATGAGTTTTCAACTCTTGCGCCTTTTTGGCGGCCAAATTCTCAAGGAGGAGTCCACCACTTTGGCGCAAGTCATCCAAACCTACGCAAAAACCAAGCAGCTAAACAAAGGCAAAGAGCTCCACGCTCACCTCCTACGCACTCATTACCCACTATGCATTTTCCTCACCAACCACCTCCTCAACATGTACTCCAAATGCGGGGACGTCGATTATGCCCTCAAGGTTTTCGACCAAATGCCTCACCGAAACTTGGTTTCCTGGACTGCAATGGTCACTGGGTTTTCTCAGAACTTGAGGTTCTCCGAGAGTTTCAAGGCCTTTTCCCAGATGAGAATTGCTGGGGAGAGCCCGACCCAGTTTGCATTTGCAAGTGTCATCAGAGCTTGTGTGGTTCTTGGGTCGGTTGAGATTGGGAGGCAGCTGCATTCTCTTGCTTTGAAATTGGGCTTGGCTTGTGAGCTGTTTGTGGGTAGTAGTTTAGCGGATATGTATTCGAAATGTGGGTTTATGGTTGAGGCTTGTAAGGTTTTCGAGGAAATGCCGAGTAAGGATGCTGTATCGTGGACTTCGATGATTGATGGGAGATATGTTGACCCTGAGCGTAGAAATATTGTGTCGTATTCCTCTCTGATCAATGGTTATGTTGAGATGGATGAAATCGAGAAGGCTTTTAGTGTGTCTGTTGACTTACAAAAGAGGGGAGTTGAGCCTAATCAGTTCACTTTCTCTAGCTTGGTCAAGGCCTGTGCCAACCAAGCTGCACTTGAACGAGGGATTCAACTTCATGCTCAGGTTGTCAAGTTTAATTTTGATAAAGACAGTTTTGTGTCTTCTGTTCTTGTTGATATTCATGGGAAATTTCATGGCTTAGGAAATGACGCCTTGGAAACTTTTAGTAGAATGGTCCAGGCAGGAGTGAAACCAAATGCAATAACATTTATCAGTCTTTTAACAGGGCTGGAAGGCTTGAAGAGGCCGAAGATTTTATTAACAGCATGCCAATGCAGCCAAATGCTTTCGGGTGGTGCCCCTTTCTTGGTGCCTGCACGATTCATGGTGATAAAGAGGGGCATACTGGCAGCGGAGAAATTGATACAGCTTGAACGTGAGAATAGTGGAGCAGATGTTTTGCTTTCAACTCTACATGCAAAGGAGCAAAAATGGGAAGATTTCAGAAGTGTGCGGAAGATGATGAGAGACAGCAGCATGAAGAAATTTCCCGGTTATAGTTGGGTTGATGTTGGAAACAAAACACACACGTCTGGAGACGAAGATTTGTCTCATCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

429

Amino Acids

47.91

Weight (kDa)

8.72

Isoelectric Point (pI)

38.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 202 - 251 3.2e-13 PPR repeat family
E_motif PF20431 356 - 413 5.5e-13 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 207
AccB1I GGYRCC 2 cut(s) 1025, 1039
AciI CCGC 5 cut(s) 30, 198, 482, 1082, 1183
AcoI YGGCCR 1 cut(s) 31
AcsI RAATTY 3 cut(s) 36, 857, 1214
AcyI GRCGYC 2 cut(s) 204, 879
AfaI GTAC 1 cut(s) 188
AfiI CCNNNNNNNGG 3 cut(s) 198, 250, 1036
AflIII ACRYGT 2 cut(s) 183, 1257
AgsI TTSAA 8 cut(s) 11, 313, 439, 767, 779, 970, 1103, 1137
AjiI CACGTC 1 cut(s) 1260
AjnI CCWGG 2 cut(s) 257, 906
AjuI GAANNNNNNNTTGG 2 cut(s) 431, 463
AluBI AGCT 8 cut(s) 100, 118, 383, 421, 460, 732, 758, 1099
AluI AGCT 8 cut(s) 100, 118, 383, 421, 460, 732, 758, 1099
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 3 cut(s) 1191, 1260, 1284
AoxI GGCC 4 cut(s) 31, 315, 741, 974
ApeKI GCWGC 7 cut(s) 97, 418, 421, 758, 1005, 1079, 1203
ApoI RAATTY 3 cut(s) 36, 857, 1214
AspLEI GCGC 2 cut(s) 21, 67
AspS9I GGNCC 1 cut(s) 904
AsuC2I CCSGG 1 cut(s) 1221
AsuHPI GGTGA 4 cut(s) 119, 157, 233, 1069
AsuII TTCGAA 1 cut(s) 494
AvaII GGWCC 1 cut(s) 904
BaeGI GKGCMC 1 cut(s) 1030
BanI GGYRCC 2 cut(s) 1025, 1039
BanII GRGCYC 2 cut(s) 120, 350
BbsI GAAGAC 1 cut(s) 822
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 7 cut(s) 109, 408, 430, 745, 1017, 1091, 1215
BccI CCATC 2 cut(s) 572, 643
BciT130I CCWGG 2 cut(s) 259, 908
BclI TGATCA 1 cut(s) 630
BcnI CCSGG 1 cut(s) 1221
BcoDI GTCTC 3 cut(s) 1191, 1260, 1284
BfaI CTAG 1 cut(s) 729
BglI GCCNNNNNGGC 1 cut(s) 27
BisI GCNGC 8 cut(s) 31, 98, 419, 422, 759, 1006, 1080, 1204
BlsI GCNGC 8 cut(s) 32, 99, 420, 423, 760, 1007, 1081, 1205
Bme1390I CCNGG 3 cut(s) 259, 908, 1221
Bme18I GGWCC 1 cut(s) 904
BmgBI CACGTC 1 cut(s) 1260
BmgT120I GGNCC 1 cut(s) 904
BmiI GGNNCC 2 cut(s) 1027, 1041
BmrFI CCNGG 3 cut(s) 259, 908, 1221
BmrI ACTGGG 2 cut(s) 285, 349
BmsI GCATC 1 cut(s) 541
BmuI ACTGGG 2 cut(s) 285, 349
BpiI GAAGAC 1 cut(s) 822
BpmI CTGGAG 1 cut(s) 1284
Bpu10I CCTNAGC 3 cut(s) 597, 789, 868
Bpu14I TTCGAA 1 cut(s) 494
BpuEI CTTGAG 3 cut(s) 26, 203, 313
BpuMI CCSGG 1 cut(s) 1221
BsaHI GRCGYC 2 cut(s) 204, 879
BsaJI CCNNGG 1 cut(s) 882
BsaXI ACNNNNNCTCC 4 cut(s) 173, 203, 1110, 1140
Bsc4I CCNNNNNNNGG 3 cut(s) 198, 250, 1036
Bse1I ACTGG 3 cut(s) 280, 355, 1080
Bse3DI GCAATG 1 cut(s) 273
BseBI CCWGG 2 cut(s) 259, 908
BseDI CCNNGG 1 cut(s) 882
BseGI GGATG 4 cut(s) 72, 556, 658, 1282
BseLI CCNNNNNNNGG 3 cut(s) 198, 250, 1036
BseMI GCAATG 1 cut(s) 273
BseMII CTCAG 3 cut(s) 299, 588, 803
BseNI ACTGG 3 cut(s) 280, 355, 1080
BseRI GAGGAG 2 cut(s) 62, 167
BseSI GKGCMC 1 cut(s) 1030
BseXI GCAGC 7 cut(s) 109, 408, 430, 745, 1017, 1091, 1215
BseYI CCCAGC 1 cut(s) 338
BsgI GTGCAG 2 cut(s) 744, 1029
BshFI GGCC 4 cut(s) 33, 317, 743, 976
BshNI GGYRCC 2 cut(s) 1025, 1039
BsiHKAI GWGCWC 1 cut(s) 120
BsiSI CCGG 1 cut(s) 1221
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 3 cut(s) 198, 250, 1036
BsmAI GTCTC 3 cut(s) 1191, 1260, 1284
BsmBI CGTCTC 1 cut(s) 1260
BsmFI GGGAC 1 cut(s) 215
BsmI GAATGC 1 cut(s) 424
BsnI GGCC 4 cut(s) 33, 317, 743, 976
Bsp119I TTCGAA 1 cut(s) 494
Bsp1286I GDGCHC 3 cut(s) 120, 350, 1030
Bsp143I GATC 1 cut(s) 630
BspACI CCGC 5 cut(s) 30, 198, 482, 1082, 1183
BspANI GGCC 4 cut(s) 33, 317, 743, 976
BspCNI CTCAG 3 cut(s) 298, 589, 802
BspLI GGNNCC 2 cut(s) 1027, 1041
BspT104I TTCGAA 1 cut(s) 494
BspT107I GGYRCC 2 cut(s) 1025, 1039
BsrDI GCAATG 1 cut(s) 273
BsrI ACTGG 3 cut(s) 280, 355, 1080
BssECI CCNNGG 1 cut(s) 882
BssMI GATC 1 cut(s) 630
BssNI GRCGYC 2 cut(s) 204, 879
BssT1I CCWWGG 1 cut(s) 882
Bst2UI CCWGG 2 cut(s) 259, 908
Bst4CI ACNGT 1 cut(s) 821
Bst6I CTCTTC 1 cut(s) 965
BstACI GRCGYC 2 cut(s) 204, 879
BstBI TTCGAA 1 cut(s) 494
BstC8I GCNNGC 2 cut(s) 997, 1044
BstDEI CTNAG 5 cut(s) 285, 597, 789, 868, 1287
BstF5I GGATG 4 cut(s) 72, 556, 658, 1282
BstHHI GCGC 2 cut(s) 21, 67
BstKTI GATC 1 cut(s) 633
BstMAI GTCTC 3 cut(s) 1191, 1260, 1284
BstMBI GATC 1 cut(s) 630
BstMWI GCNNNNNNNGC 3 cut(s) 27, 755, 1005
BstNI CCWGG 2 cut(s) 259, 908
BstNSI RCATGY 3 cut(s) 187, 999, 1148
BstSCI CCNGG 3 cut(s) 257, 906, 1219
BstSLI GKGCMC 1 cut(s) 1030
BstV1I GCAGC 7 cut(s) 109, 408, 430, 745, 1017, 1091, 1215
BstV2I GAAGAC 1 cut(s) 822
BstXI CCANNNNNNTGG 1 cut(s) 61
BsuRI GGCC 4 cut(s) 33, 317, 743, 976
BtrI CACGTC 1 cut(s) 1260
BtsCI GGATG 4 cut(s) 72, 556, 658, 1282
BtsIMutI CAGTG 1 cut(s) 273
Cac8I GCNNGC 2 cut(s) 997, 1044
CfoI GCGC 2 cut(s) 21, 67
Cfr13I GGNCC 1 cut(s) 904
CseI GACGC 1 cut(s) 887
Csp6I GTAC 1 cut(s) 187
CviAII CATG 8 cut(s) 184, 785, 851, 863, 996, 1054, 1145, 1207
CviQI GTAC 1 cut(s) 187
DdeI CTNAG 5 cut(s) 285, 597, 789, 868, 1287
DpnI GATC 1 cut(s) 632
DpnII GATC 1 cut(s) 630
EaeI YGGCCR 1 cut(s) 31
Eam1104I CTCTTC 1 cut(s) 965
EarI CTCTTC 1 cut(s) 965
Ecl136II GAGCTC 1 cut(s) 118
Eco130I CCWWGG 1 cut(s) 882
Eco147I AGGCCT 2 cut(s) 317, 743
Eco24I GRGCYC 2 cut(s) 120, 350
Eco47I GGWCC 1 cut(s) 904
Eco53kI GAGCTC 1 cut(s) 118
EcoICRI GAGCTC 1 cut(s) 118
EcoRII CCWGG 2 cut(s) 257, 906
EcoT14I CCWWGG 1 cut(s) 882
EcoT22I ATGCAT 1 cut(s) 158
EcoT38I GRGCYC 2 cut(s) 120, 350
ErhI CCWWGG 1 cut(s) 882
Esp3I CGTCTC 1 cut(s) 1260
FaeI CATG 8 cut(s) 187, 788, 854, 866, 999, 1057, 1148, 1210
FalI AAGNNNNNCTT 2 cut(s) 747, 779
FaqI GGGAC 1 cut(s) 215
FatI CATG 8 cut(s) 183, 784, 850, 862, 995, 1053, 1144, 1206
FauI CCCGC 1 cut(s) 191
FbaI TGATCA 1 cut(s) 630
Fnu4HI GCNGC 8 cut(s) 31, 98, 419, 422, 759, 1006, 1080, 1204
FokI GGATG 4 cut(s) 59, 563, 665, 1269
FriOI GRGCYC 2 cut(s) 120, 350
Fsp4HI GCNGC 8 cut(s) 31, 98, 419, 422, 759, 1006, 1080, 1204
FspBI CTAG 1 cut(s) 729
GlaI GCGC 2 cut(s) 20, 66
GluI GCNGC 8 cut(s) 31, 98, 419, 422, 759, 1006, 1080, 1204
GsaI CCCAGC 1 cut(s) 342
GsuI CTGGAG 1 cut(s) 1284
HaeIII GGCC 4 cut(s) 33, 317, 743, 976
HapII CCGG 1 cut(s) 1221
HgaI GACGC 1 cut(s) 887
HhaI GCGC 2 cut(s) 21, 67
Hin1I GRCGYC 2 cut(s) 204, 879
Hin1II CATG 8 cut(s) 187, 788, 854, 866, 999, 1057, 1148, 1210
Hin6I GCGC 2 cut(s) 19, 65
HinP1I GCGC 2 cut(s) 19, 65
HincII GTYRAC 2 cut(s) 592, 685
HindII GTYRAC 2 cut(s) 592, 685
HinfI GANTC 3 cut(s) 50, 775, 1050
HpaII CCGG 1 cut(s) 1221
HphI GGTGA 4 cut(s) 119, 157, 233, 1069
Hpy166II GTNNAC 5 cut(s) 54, 564, 592, 685, 720
Hpy188I TCNGA 5 cut(s) 288, 304, 380, 630, 1175
Hpy188III TCNNGA 2 cut(s) 661, 1263
Hpy8I GTNNAC 5 cut(s) 54, 564, 592, 685, 720
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 2 cut(s) 658, 956
HpyCH4III ACNGT 1 cut(s) 821
HpyCH4IV ACGT 3 cut(s) 204, 1105, 1259
HpyF10VI GCNNNNNNNGC 3 cut(s) 27, 755, 1005
HpyF3I CTNAG 5 cut(s) 285, 597, 789, 868, 1287
HpySE526I ACGT 3 cut(s) 204, 1105, 1259
Hsp92I GRCGYC 2 cut(s) 204, 879
Hsp92II CATG 8 cut(s) 187, 788, 854, 866, 999, 1057, 1148, 1210
HspAI GCGC 2 cut(s) 19, 65
Ksp22I TGATCA 1 cut(s) 630
Kzo9I GATC 1 cut(s) 630
LmnI GCTCC 3 cut(s) 123, 1118, 1153
Lsp1109I GCAGC 7 cut(s) 109, 408, 430, 745, 1017, 1091, 1215
LweI GCATC 1 cut(s) 541
MaeI CTAG 1 cut(s) 729
MaeII ACGT 3 cut(s) 204, 1105, 1259
MaeIII GTNAC 1 cut(s) 271
MalI GATC 1 cut(s) 632
MboI GATC 1 cut(s) 630
MboII GAAGA 7 cut(s) 822, 982, 991, 1178, 1198, 1222, 1283
MhlI GDGCHC 3 cut(s) 120, 350, 1030
MluCI AATT 7 cut(s) 36, 333, 440, 805, 857, 1088, 1214
MlyI GAGTC 1 cut(s) 59
MmeI TCCRAC 1 cut(s) 1222
Mph1103I ATGCAT 1 cut(s) 158
MseI TTAA 3 cut(s) 804, 950, 989
MslI CAYNNNNRTG 1 cut(s) 1000
MspA1I CMGCKG 2 cut(s) 421, 1082
MspI CCGG 1 cut(s) 1221
MspR9I CCNGG 3 cut(s) 259, 908, 1221
Mva1269I GAATGC 1 cut(s) 424
MvaI CCWGG 2 cut(s) 259, 908
MwoI GCNNNNNNNGC 3 cut(s) 27, 755, 1005
NciI CCSGG 1 cut(s) 1221
NdeII GATC 1 cut(s) 630
NlaIII CATG 8 cut(s) 187, 788, 854, 866, 999, 1057, 1148, 1210
NlaIV GGNNCC 2 cut(s) 1027, 1041
NmeAIII GCCGAG 1 cut(s) 567
NmuCI GTSAC 1 cut(s) 271
NsiI ATGCAT 1 cut(s) 158
NspI RCATGY 3 cut(s) 187, 999, 1148
NspV TTCGAA 1 cut(s) 494
PaeI GCATGC 1 cut(s) 999
PceI AGGCCT 2 cut(s) 317, 743
PciI ACATGT 1 cut(s) 183
PcsI WCGNNNNNNNCGW 1 cut(s) 566
PctI GAATGC 1 cut(s) 424
PfeI GAWTC 2 cut(s) 775, 1050
PfoI TCCNGGA 1 cut(s) 257
PkrI GCNGC 8 cut(s) 32, 99, 420, 423, 760, 1007, 1081, 1205
PleI GAGTC 1 cut(s) 58
PpsI GAGTC 1 cut(s) 58
PscI ACATGT 1 cut(s) 183
Psp124BI GAGCTC 1 cut(s) 120
Psp6I CCWGG 2 cut(s) 257, 906
PspFI CCCAGC 1 cut(s) 338
PspGI CCWGG 2 cut(s) 257, 906
PspN4I GGNNCC 2 cut(s) 1027, 1041
PspPI GGNCC 1 cut(s) 904
PvuII CAGCTG 1 cut(s) 421
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
RseI CAYNNNNRTG 1 cut(s) 1000
SacI GAGCTC 1 cut(s) 120
SaqAI TTAA 3 cut(s) 804, 950, 989
SatI GCNGC 8 cut(s) 31, 98, 419, 422, 759, 1006, 1080, 1204
Sau3AI GATC 1 cut(s) 630
Sau96I GGNCC 1 cut(s) 904
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 3 cut(s) 259, 908, 1221
SduI GDGCHC 3 cut(s) 120, 350, 1030
SfaNI GCATC 1 cut(s) 541
SfuI TTCGAA 1 cut(s) 494
SinI GGWCC 1 cut(s) 904
SmiMI CAYNNNNRTG 1 cut(s) 1000
SmlI CTYRAG 3 cut(s) 41, 218, 292
SmoI CTYRAG 3 cut(s) 41, 218, 292
SphI GCATGC 1 cut(s) 999
Sse9I AATT 7 cut(s) 36, 333, 440, 805, 857, 1088, 1214
SseBI AGGCCT 2 cut(s) 317, 743
SsiI CCGC 5 cut(s) 30, 198, 482, 1082, 1183
SspI AATATT 1 cut(s) 610
SspMI CTAG 1 cut(s) 729
SstI GAGCTC 1 cut(s) 120
StuI AGGCCT 2 cut(s) 317, 743
StyD4I CCNGG 3 cut(s) 257, 906, 1219
StyI CCWWGG 1 cut(s) 882
TaaI ACNGT 1 cut(s) 821
TaiI ACGT 3 cut(s) 207, 1108, 1262
TaqI TCGA 6 cut(s) 207, 228, 494, 531, 569, 660
TasI AATT 7 cut(s) 36, 333, 440, 805, 857, 1088, 1214
TatI WGTACW 1 cut(s) 186
TauI GCSGC 1 cut(s) 33
TfiI GAWTC 2 cut(s) 775, 1050
Tru1I TTAA 3 cut(s) 804, 950, 989
Tru9I TTAA 3 cut(s) 804, 950, 989
TscAI CASTG 1 cut(s) 280
TseFI GTSAC 1 cut(s) 271
TseI GCWGC 7 cut(s) 97, 418, 421, 758, 1005, 1079, 1203
Tsp45I GTSAC 1 cut(s) 271
TspDTI ATGAA 6 cut(s) 669, 773, 839, 851, 1042, 1223
TspRI CASTG 1 cut(s) 280
VpaK11BI GGWCC 1 cut(s) 904
XapI RAATTY 3 cut(s) 36, 857, 1214
XceI RCATGY 3 cut(s) 187, 999, 1148
XspI CTAG 1 cut(s) 729
ZraI GACGTC 1 cut(s) 205
Zsp2I ATGCAT 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.