Rh4BG423800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000783
Physical Location & Seq
Forward (+)
56828964 .. 56829317
354 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG423800.1

Sequence Viewer

Length: 354 bp
ATGTCTCGAGCTGACCTCTACCCTAGACGGTCTTTTGGTTCGAAGGTGATTGTGGCGACTGCCAGTGGTTTTGAACGTAATGCTAGCTTTCAGCCTTCATCTGGAGGCAGTGACTGCGTCTGGTTGGGATCTGTTCTCCTTGGAGATGAGGGCAGCGATCTGTTGATGGTGAAGGATGGTGATTTGGCTCGATCTGTTTTCATTGACGATGTTGGCGATTGGGGTGGGAATCGGCTTTTTCGTGGTGGCCTAGCAGTGGCTCTGTGGCAGAGTTTTGCTGTGGCAAGGCTAGTTAGGGGTAGTGGTGGGGCGCCCTTAGCAATTCTTGGGGTGCCCTTCGCAATTGCCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

117

Amino Acids

12.24

Weight (kDa)

6.12

Isoelectric Point (pI)

44.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 310, 331
AclWI GGATC 1 cut(s) 136
AcyI GRCGYC 1 cut(s) 311
AfiI CCNNNNNNNGG 2 cut(s) 101, 256
AgsI TTSAA 1 cut(s) 74
AluBI AGCT 2 cut(s) 11, 87
AluI AGCT 2 cut(s) 11, 87
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 1 cut(s) 136
AlwNI CAGNNNCTG 1 cut(s) 114
Ama87I CYCGRG 1 cut(s) 6
AoxI GGCC 1 cut(s) 247
ApeKI GCWGC 1 cut(s) 153
AspLEI GCGC 1 cut(s) 313
AsuHPI GGTGA 3 cut(s) 58, 181, 191
AsuII TTCGAA 1 cut(s) 41
AsuNHI GCTAGC 1 cut(s) 83
AvaI CYCGRG 1 cut(s) 6
BaeGI GKGCMC 1 cut(s) 336
BanI GGYRCC 2 cut(s) 310, 331
BbvI GCAGC 1 cut(s) 165
BccI CCATC 2 cut(s) 160, 170
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 4 cut(s) 24, 84, 251, 290
BfoI RGCGCY 1 cut(s) 314
BisI GCNGC 1 cut(s) 154
BlsI GCNGC 1 cut(s) 155
BmeT110I CYCGRG 1 cut(s) 6
BmiI GGNNCC 2 cut(s) 312, 333
BmtI GCTAGC 1 cut(s) 87
BplI GAGNNNNNCTC 1 cut(s) 32
BpmI CTGGAG 1 cut(s) 123
Bpu10I CCTNAGC 1 cut(s) 316
Bpu14I TTCGAA 1 cut(s) 41
BsaHI GRCGYC 1 cut(s) 311
BsaJI CCNNGG 1 cut(s) 139
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 256
Bse1I ACTGG 1 cut(s) 63
BseDI CCNNGG 1 cut(s) 139
BseGI GGATG 1 cut(s) 181
BseLI CCNNNNNNNGG 2 cut(s) 101, 256
BseNI ACTGG 1 cut(s) 63
BseSI GKGCMC 1 cut(s) 336
BseXI GCAGC 1 cut(s) 165
BshFI GGCC 1 cut(s) 249
BshNI GGYRCC 2 cut(s) 310, 331
BsiHKCI CYCGRG 1 cut(s) 6
BslI CCNNNNNNNGG 2 cut(s) 101, 256
BsmAI GTCTC 1 cut(s) 9
BsnI GGCC 1 cut(s) 249
BsoBI CYCGRG 1 cut(s) 6
Bsp119I TTCGAA 1 cut(s) 41
Bsp1286I GDGCHC 1 cut(s) 336
Bsp143I GATC 3 cut(s) 128, 157, 191
BspANI GGCC 1 cut(s) 249
BspLI GGNNCC 2 cut(s) 312, 333
BspOI GCTAGC 1 cut(s) 87
BspPI GGATC 1 cut(s) 136
BspT104I TTCGAA 1 cut(s) 41
BspT107I GGYRCC 2 cut(s) 310, 331
BsrI ACTGG 1 cut(s) 63
BssECI CCNNGG 1 cut(s) 139
BssMI GATC 3 cut(s) 128, 157, 191
BssNI GRCGYC 1 cut(s) 311
BssT1I CCWWGG 1 cut(s) 139
Bst4CI ACNGT 1 cut(s) 30
BstACI GRCGYC 1 cut(s) 311
BstAPI GCANNNNNTGC 1 cut(s) 114
BstBI TTCGAA 1 cut(s) 41
BstC8I GCNNGC 1 cut(s) 85
BstDEI CTNAG 1 cut(s) 316
BstF5I GGATG 1 cut(s) 181
BstH2I RGCGCY 1 cut(s) 314
BstHHI GCGC 1 cut(s) 313
BstKTI GATC 3 cut(s) 131, 160, 194
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 3 cut(s) 128, 157, 191
BstMWI GCNNNNNNNGC 2 cut(s) 114, 317
BstSLI GKGCMC 1 cut(s) 336
BstV1I GCAGC 1 cut(s) 165
BstX2I RGATCY 1 cut(s) 128
BstYI RGATCY 1 cut(s) 128
BsuRI GGCC 1 cut(s) 249
BtsCI GGATG 1 cut(s) 181
BtsI GCAGTG 2 cut(s) 115, 261
BtsIMutI CAGTG 3 cut(s) 70, 115, 261
Cac8I GCNNGC 1 cut(s) 85
CaiI CAGNNNCTG 1 cut(s) 114
CfoI GCGC 1 cut(s) 313
CseI GACGC 1 cut(s) 106
CviAII CATG 1 cut(s) 349
CviJI RGCY 8 cut(s) 11, 87, 94, 188, 235, 249, 260, 289
CviKI_1 RGCY 8 cut(s) 11, 87, 94, 188, 235, 249, 260, 289
DdeI CTNAG 1 cut(s) 316
DinI GGCGCC 1 cut(s) 312
DpnI GATC 3 cut(s) 130, 159, 193
DpnII GATC 3 cut(s) 128, 157, 191
Eco130I CCWWGG 1 cut(s) 139
Eco88I CYCGRG 1 cut(s) 6
EcoT14I CCWWGG 1 cut(s) 139
EgeI GGCGCC 1 cut(s) 312
EheI GGCGCC 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 139
FaeI CATG 1 cut(s) 352
FaiI YATR 1 cut(s) 350
FatI CATG 1 cut(s) 348
Fnu4HI GCNGC 1 cut(s) 154
FokI GGATG 1 cut(s) 188
Fsp4HI GCNGC 1 cut(s) 154
FspBI CTAG 4 cut(s) 24, 84, 251, 290
GlaI GCGC 1 cut(s) 312
GluI GCNGC 1 cut(s) 154
GsuI CTGGAG 1 cut(s) 123
HaeII RGCGCY 1 cut(s) 314
HaeIII GGCC 1 cut(s) 249
HgaI GACGC 1 cut(s) 106
HhaI GCGC 1 cut(s) 313
Hin1I GRCGYC 1 cut(s) 311
Hin1II CATG 1 cut(s) 352
Hin6I GCGC 1 cut(s) 311
HinP1I GCGC 1 cut(s) 311
HinfI GANTC 1 cut(s) 229
HphI GGTGA 3 cut(s) 58, 181, 191
Hpy188III TCNNGA 2 cut(s) 6, 102
HpyAV CCTTC 4 cut(s) 37, 105, 166, 346
HpyCH4III ACNGT 1 cut(s) 30
HpyCH4IV ACGT 1 cut(s) 76
HpyF10VI GCNNNNNNNGC 2 cut(s) 114, 317
HpyF3I CTNAG 1 cut(s) 316
HpySE526I ACGT 1 cut(s) 76
Hsp92I GRCGYC 1 cut(s) 311
Hsp92II CATG 1 cut(s) 352
HspAI GCGC 1 cut(s) 311
KasI GGCGCC 1 cut(s) 310
Kzo9I GATC 3 cut(s) 128, 157, 191
LpnPI CCDG 3 cut(s) 76, 87, 106
Lsp1109I GCAGC 1 cut(s) 165
MaeI CTAG 4 cut(s) 24, 84, 251, 290
MaeII ACGT 1 cut(s) 76
MaeIII GTNAC 1 cut(s) 110
MalI GATC 3 cut(s) 130, 159, 193
MboI GATC 3 cut(s) 128, 157, 191
MfeI CAATTG 1 cut(s) 342
MflI RGATCY 1 cut(s) 128
MhlI GDGCHC 1 cut(s) 336
MluCI AATT 2 cut(s) 321, 342
Mly113I GGCGCC 1 cut(s) 311
MnlI CCTC 3 cut(s) 26, 98, 142
MunI CAATTG 1 cut(s) 342
MwoI GCNNNNNNNGC 2 cut(s) 114, 317
NarI GGCGCC 1 cut(s) 311
NdeII GATC 3 cut(s) 128, 157, 191
NheI GCTAGC 1 cut(s) 83
NlaIII CATG 1 cut(s) 352
NlaIV GGNNCC 2 cut(s) 312, 333
NmuCI GTSAC 1 cut(s) 110
NspV TTCGAA 1 cut(s) 41
PaeR7I CTCGAG 1 cut(s) 6
PcsI WCGNNNNNNNCGW 2 cut(s) 213, 238
PfeI GAWTC 1 cut(s) 229
PflFI GACNNNGTC 1 cut(s) 116
PkrI GCNGC 1 cut(s) 155
PluTI GGCGCC 1 cut(s) 314
PspN4I GGNNCC 2 cut(s) 312, 333
PstNI CAGNNNCTG 1 cut(s) 114
PsuI RGATCY 1 cut(s) 128
PsyI GACNNNGTC 1 cut(s) 116
SatI GCNGC 1 cut(s) 154
Sau3AI GATC 3 cut(s) 128, 157, 191
SduI GDGCHC 1 cut(s) 336
SetI ASST 5 cut(s) 13, 18, 48, 79, 89
SfoI GGCGCC 1 cut(s) 312
Sfr274I CTCGAG 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 41
SlaI CTCGAG 1 cut(s) 6
SmlI CTYRAG 1 cut(s) 6
SmoI CTYRAG 1 cut(s) 6
Sse9I AATT 2 cut(s) 321, 342
SspDI GGCGCC 1 cut(s) 310
SspMI CTAG 4 cut(s) 24, 84, 251, 290
StyI CCWWGG 1 cut(s) 139
TaaI ACNGT 1 cut(s) 30
TaiI ACGT 1 cut(s) 79
TaqI TCGA 3 cut(s) 7, 41, 190
TasI AATT 2 cut(s) 321, 342
TfiI GAWTC 1 cut(s) 229
TscAI CASTG 3 cut(s) 70, 115, 261
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 1 cut(s) 153
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 2 cut(s) 87, 190
TspRI CASTG 3 cut(s) 70, 115, 261
Tth111I GACNNNGTC 1 cut(s) 116
XhoI CTCGAG 1 cut(s) 6
XspI CTAG 4 cut(s) 24, 84, 251, 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.