Rh4AG080800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000783
Physical Location & Seq
Forward (+)
16935349 .. 16943834
8486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG080800.1

Sequence Viewer

Length: 381 bp
ATGGTAGTGGTGCAGGTTTCAAGGCAGGTGCAGTGGGTTGCCTTTTGGTTCATCACGACGGAGGATCTGATCTTCGATGGTGGGGGTGGTACTTCGTCCGGAGGCGGTGAAGGCTTCTGGTTGAAATTTGACCCCCTTGAAAATGGGGCAGAAGGTGGAGGTGCAGTGATGCAGCTGGCTGTACTTGAAAGGGATGCTGGCGGAGGTTCTGGGGTGGATGATCGGAGATCTACGGTGACGGAGGTCGACGGTGACATACGATCGGAGTGGGTTGCCTTGTCTCCTTGCTGGGCAGATTGCTTCTGGGATCCTTGCTTTGGGCTTTTTGGGCTAAGGTTGACAGGATGGGCTTTGTCCCATGGCTGGCAGTGGGTAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

126

Amino Acids

13.63

Weight (kDa)

4.18

Isoelectric Point (pI)

33.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 16
Acc36I ACCTGC 2 cut(s) 4, 16
AccI GTMKAC 1 cut(s) 246
AccIII TCCGGA 1 cut(s) 98
AciI CCGC 2 cut(s) 105, 201
AclWI GGATC 3 cut(s) 72, 302, 315
AcsI RAATTY 1 cut(s) 125
AfaI GTAC 2 cut(s) 91, 183
AfiI CCNNNNNNNGG 2 cut(s) 317, 363
AgsI TTSAA 4 cut(s) 21, 124, 140, 188
AluBI AGCT 1 cut(s) 175
AluI AGCT 1 cut(s) 175
Alw26I GTCTC 1 cut(s) 285
AlwI GGATC 3 cut(s) 72, 302, 315
Aor13HI TCCGGA 1 cut(s) 98
ApeKI GCWGC 1 cut(s) 172
ApoI RAATTY 1 cut(s) 125
AsuHPI GGTGA 3 cut(s) 119, 247, 263
BamHI GGATCC 1 cut(s) 307
BbvI GCAGC 1 cut(s) 184
BccI CCATC 2 cut(s) 71, 339
BcoDI GTCTC 1 cut(s) 285
BfaI CTAG 1 cut(s) 379
BfuAI ACCTGC 2 cut(s) 4, 16
BglII AGATCT 1 cut(s) 227
BisI GCNGC 1 cut(s) 173
BlsI GCNGC 1 cut(s) 174
BmiI GGNNCC 1 cut(s) 309
BmsI GCATC 2 cut(s) 159, 184
BoxI GACNNNNGTC 1 cut(s) 242
Bpu10I CCTNAGC 1 cut(s) 332
BsaJI CCNNGG 1 cut(s) 358
BsaWI WCCGGW 1 cut(s) 98
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bsc4I CCNNNNNNNGG 2 cut(s) 317, 363
BseAI TCCGGA 1 cut(s) 98
BseDI CCNNGG 1 cut(s) 358
BseGI GGATG 3 cut(s) 199, 223, 350
BseLI CCNNNNNNNGG 2 cut(s) 317, 363
BseXI GCAGC 1 cut(s) 184
BseYI CCCAGC 1 cut(s) 288
BsgI GTGCAG 3 cut(s) 32, 50, 183
Bsh1285I CGRYCG 1 cut(s) 263
BsiEI CGRYCG 1 cut(s) 263
BsiSI CCGG 1 cut(s) 99
BslFI GGGAC 1 cut(s) 340
BslI CCNNNNNNNGG 2 cut(s) 317, 363
BsmAI GTCTC 1 cut(s) 285
BsmFI GGGAC 1 cut(s) 340
Bsp13I TCCGGA 1 cut(s) 98
Bsp143I GATC 6 cut(s) 64, 69, 220, 227, 260, 307
Bsp19I CCATGG 1 cut(s) 358
BspACI CCGC 2 cut(s) 105, 201
BspEI TCCGGA 1 cut(s) 98
BspLI GGNNCC 1 cut(s) 309
BspMI ACCTGC 2 cut(s) 4, 16
BspPI GGATC 3 cut(s) 72, 302, 315
BssECI CCNNGG 1 cut(s) 358
BssMI GATC 6 cut(s) 64, 69, 220, 227, 260, 307
BssT1I CCWWGG 1 cut(s) 358
Bst4CI ACNGT 2 cut(s) 235, 251
BstC8I GCNNGC 3 cut(s) 177, 199, 365
BstDEI CTNAG 1 cut(s) 332
BstDSI CCRYGG 1 cut(s) 358
BstF5I GGATG 3 cut(s) 199, 223, 350
BstKTI GATC 6 cut(s) 67, 72, 223, 230, 263, 310
BstMAI GTCTC 1 cut(s) 285
BstMBI GATC 6 cut(s) 64, 69, 220, 227, 260, 307
BstMCI CGRYCG 1 cut(s) 263
BstMWI GCNNNNNNNGC 2 cut(s) 111, 328
BstPAI GACNNNNGTC 1 cut(s) 242
BstV1I GCAGC 1 cut(s) 184
BstX2I RGATCY 3 cut(s) 64, 227, 307
BstYI RGATCY 3 cut(s) 64, 227, 307
BtgI CCRYGG 1 cut(s) 358
BtsCI GGATG 3 cut(s) 199, 223, 350
BtsI GCAGTG 3 cut(s) 38, 171, 374
BtsIMutI CAGTG 3 cut(s) 38, 171, 374
BveI ACCTGC 2 cut(s) 4, 16
Cac8I GCNNGC 3 cut(s) 177, 199, 365
Csp6I GTAC 2 cut(s) 90, 182
CviAII CATG 1 cut(s) 359
CviJI RGCY 8 cut(s) 114, 175, 179, 322, 331, 350, 363, 378
CviKI_1 RGCY 8 cut(s) 114, 175, 179, 322, 331, 350, 363, 378
CviQI GTAC 2 cut(s) 90, 182
DdeI CTNAG 1 cut(s) 332
DpnI GATC 6 cut(s) 66, 71, 222, 229, 262, 309
DpnII GATC 6 cut(s) 64, 69, 220, 227, 260, 307
EciI GGCGGA 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 358
EcoT14I CCWWGG 1 cut(s) 358
ErhI CCWWGG 1 cut(s) 358
FaeI CATG 1 cut(s) 362
FaiI YATR 2 cut(s) 257, 360
FaqI GGGAC 1 cut(s) 340
FatI CATG 1 cut(s) 358
FblI GTMKAC 1 cut(s) 246
Fnu4HI GCNGC 1 cut(s) 173
FokI GGATG 3 cut(s) 206, 230, 357
Fsp4HI GCNGC 1 cut(s) 173
FspBI CTAG 1 cut(s) 379
GluI GCNGC 1 cut(s) 173
GsaI CCCAGC 1 cut(s) 292
HapII CCGG 1 cut(s) 99
Hin1II CATG 1 cut(s) 362
HincII GTYRAC 2 cut(s) 247, 339
HindII GTYRAC 2 cut(s) 247, 339
HpaII CCGG 1 cut(s) 99
HphI GGTGA 3 cut(s) 119, 247, 263
Hpy166II GTNNAC 2 cut(s) 247, 339
Hpy188I TCNGA 3 cut(s) 69, 225, 265
Hpy188III TCNNGA 2 cut(s) 55, 99
Hpy8I GTNNAC 2 cut(s) 247, 339
Hpy99I CGWCG 2 cut(s) 61, 251
HpyAV CCTTC 2 cut(s) 104, 146
HpyCH4III ACNGT 2 cut(s) 235, 251
HpyCH4V TGCA 4 cut(s) 13, 31, 164, 172
HpyF10VI GCNNNNNNNGC 2 cut(s) 111, 328
HpyF3I CTNAG 1 cut(s) 332
Hsp92II CATG 1 cut(s) 362
Kpn2I TCCGGA 1 cut(s) 98
Kzo9I GATC 6 cut(s) 64, 69, 220, 227, 260, 307
Lsp1109I GCAGC 1 cut(s) 184
LweI GCATC 2 cut(s) 159, 184
MaeI CTAG 1 cut(s) 379
MaeIII GTNAC 2 cut(s) 235, 251
MalI GATC 6 cut(s) 66, 71, 222, 229, 262, 309
MboI GATC 6 cut(s) 64, 69, 220, 227, 260, 307
MboII GAAGA 1 cut(s) 64
MflI RGATCY 3 cut(s) 64, 227, 307
MluCI AATT 1 cut(s) 125
MnlI CCTC 5 cut(s) 55, 95, 152, 197, 235
MroI TCCGGA 1 cut(s) 98
MspA1I CMGCKG 1 cut(s) 175
MspI CCGG 1 cut(s) 99
MwoI GCNNNNNNNGC 2 cut(s) 111, 328
NcoI CCATGG 1 cut(s) 358
NdeII GATC 6 cut(s) 64, 69, 220, 227, 260, 307
NlaIII CATG 1 cut(s) 362
NlaIV GGNNCC 1 cut(s) 309
NmuCI GTSAC 2 cut(s) 235, 251
PaqCI CACCTGC 1 cut(s) 16
PkrI GCNGC 1 cut(s) 174
Ple19I CGATCG 1 cut(s) 263
PshAI GACNNNNGTC 1 cut(s) 242
PspFI CCCAGC 1 cut(s) 288
PspN4I GGNNCC 1 cut(s) 309
PsuI RGATCY 3 cut(s) 64, 227, 307
PvuI CGATCG 1 cut(s) 263
PvuII CAGCTG 1 cut(s) 175
RsaI GTAC 2 cut(s) 91, 183
RsaNI GTAC 2 cut(s) 90, 182
SalI GTCGAC 1 cut(s) 245
SatI GCNGC 1 cut(s) 173
Sau3AI GATC 6 cut(s) 64, 69, 220, 227, 260, 307
SetI ASST 8 cut(s) 18, 30, 157, 163, 177, 208, 246, 338
SfaNI GCATC 2 cut(s) 159, 184
Sse9I AATT 1 cut(s) 125
SsiI CCGC 2 cut(s) 105, 201
SspMI CTAG 1 cut(s) 379
StyI CCWWGG 1 cut(s) 358
TaaI ACNGT 2 cut(s) 235, 251
TaqI TCGA 2 cut(s) 75, 246
TasI AATT 1 cut(s) 125
TatI WGTACW 1 cut(s) 181
TscAI CASTG 3 cut(s) 38, 171, 374
TseFI GTSAC 2 cut(s) 235, 251
TseI GCWGC 1 cut(s) 172
Tsp45I GTSAC 2 cut(s) 235, 251
TspDTI ATGAA 1 cut(s) 40
TspGWI ACGGA 2 cut(s) 74, 254
TspRI CASTG 3 cut(s) 38, 171, 374
XapI RAATTY 1 cut(s) 125
XmiI GTMKAC 1 cut(s) 246
XspI CTAG 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.