Rmu_sc0001477.1_g000004

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001477.1
OG0000633
Physical Location & Seq
Reverse (-)
7006 .. 7755
750 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001477.1_g000004.1.cds

Sequence Viewer

Length: 750 bp
atgaatgagccagaagtgacaggagaggggagaagtgtcgcccgggcactgatattctctccgggaacttcgtcagcagaggcgccagctgttcagggggaaatccatggtctcggatccttggacccgataatcccggatcaggtgactccggtatcggaattggagtctatgagagctgagatcacagccttcagggatcgggaacaacggagtaccgatagggaaacaaatggcctcacgcaacaaagaatgcaggatttggaagatgaaaacatggcgctggcccgagcactagacaggaggcaccagcacaacgaggtactcactcaagccctggctagatcatcccagctagcaggcatgaacgctgccccgataacaggagacaccagaaccgcaccggcacctcctacggagggcagtcagaggttaatccaggtaccggtagacttattccctgaaagcttgaggcatctaccaccaccaccgttaccacagccagttcacagtatcacaccggtacccgacgtaaacacgacattactcgtacaaatgagcaactccttacatgccctgcaggcaagggtacaggccacagaaaatagagacacctggagagctcggaacagttacgaggaccgcccggggcctttcacccaacaggttaggcgagccattcgagcaaatacatcgaaaccactgaagattgactatacgggacttggagacccctaccaacatttatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

249

Amino Acids

27.46

Weight (kDa)

5.66

Isoelectric Point (pI)

49.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
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Orthologous Genes (Group: OG0000633)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22376 FvH4_4g15485
rosa_laevigata RLG00000001772
rosa_multiflora Rmu_co7980664.1_g000001 Rmu_co7991850.1_g000001 Rmu_co8069086.1_g000001 Rmu_co8203420.1_g000001 Rmu_co8213206.1_g000001 Rmu_co8240317.1_g000001 Rmu_co8382323.1_g000001 Rmu_co8439419.1_g000001 Rmu_co8463919.1_g000001 Rmu_co8496399.1_g000001 Rmu_co8505885.1_g000001 Rmu_sc0000194.1_g000007 Rmu_sc0000194.1_g000008 Rmu_sc0000279.1_g000005 Rmu_sc0000367.1_g000177 Rmu_sc0000385.1_g000009 Rmu_sc0000423.1_g000016 Rmu_sc0000539.1_g000053 Rmu_sc0000779.1_g000017 Rmu_sc0000940.1_g000038 Rmu_sc0000942.1_g000025 Rmu_sc0001436.1_g000019 Rmu_sc0001477.1_g000004 Rmu_sc0001483.1_g000028 Rmu_sc0001518.1_g000005 Rmu_sc0001565.1_g000021 Rmu_sc0001655.1_g000010 Rmu_sc0001773.1_g000009 Rmu_sc0001782.1_g000037 Rmu_sc0001866.1_g000009 Rmu_sc0002054.1_g000022 Rmu_sc0002075.1_g000001 Rmu_sc0002264.1_g000016 Rmu_sc0002868.1_g000020 Rmu_sc0003033.1_g000062 Rmu_sc0003276.1_g000007 Rmu_sc0003486.1_g000003 Rmu_sc0003760.1_g000026 Rmu_sc0004391.1_g000020 Rmu_sc0004549.1_g000021 Rmu_sc0004835.1_g000002 Rmu_sc0005137.1_g000028 Rmu_sc0005178.1_g000022 Rmu_sc0005406.1_g000004 Rmu_sc0006059.1_g000064 Rmu_sc0006822.1_g000011 Rmu_sc0006997.1_g000014 Rmu_sc0007144.1_g000004 Rmu_sc0007321.1_g000001 Rmu_sc0007677.1_g000001 Rmu_sc0009120.1_g000003 Rmu_sc0009255.1_g000002 Rmu_sc0009427.1_g000001 Rmu_sc0009806.1_g000012 Rmu_sc0010048.1_g000010 Rmu_sc0011416.1_g000001 Rmu_sc0014844.1_g000011 Rmu_sc0018644.1_g000003 Rmu_sc0019345.1_g000006 Rmu_sc0025211.1_g000005 Rmu_sc0029888.1_g000001 Rmu_sc0035796.1_g000001 Rmu_ssc0000381.1_g000017
rosa_roxburghii Rroxscaffold_1G00018250 Rroxscaffold_2G00093100 Rroxscaffold_2G00101670 Rroxscaffold_3G00249740 Rroxscaffold_4G00310550 Rroxscaffold_4G00331340 Rroxscaffold_5G00337050 Rroxscaffold_5G00378590 Rroxscaffold_5G00378600 Rroxscaffold_6G00389000 Rroxscaffold_6G00401050
rosa_rugosa Rorug02G0247700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 442, 523
AccB1I GGYRCC 5 cut(s) 82, 306, 406, 442, 523
AccI GTMKAC 1 cut(s) 450
AciI CCGC 2 cut(s) 399, 643
AclWI GGATC 4 cut(s) 111, 124, 147, 207
AcuI CTGAAG 2 cut(s) 178, 725
AcyI GRCGYC 1 cut(s) 83
AfaI GTAC 6 cut(s) 217, 324, 444, 525, 552, 591
AfiI CCNNNNNNNGG 4 cut(s) 142, 383, 419, 445
AgeI ACCGGT 2 cut(s) 445, 520
AjnI CCWGG 3 cut(s) 336, 438, 614
AluBI AGCT 5 cut(s) 89, 179, 355, 468, 623
AluI AGCT 5 cut(s) 89, 179, 355, 468, 623
Alw21I GWGCWC 2 cut(s) 295, 625
Alw26I GTCTC 4 cut(s) 116, 381, 603, 723
AlwI GGATC 4 cut(s) 111, 124, 147, 207
Ama87I CYCGRG 3 cut(s) 42, 288, 646
AoxI GGCC 4 cut(s) 235, 285, 594, 650
ApeKI GCWGC 1 cut(s) 371
AsiGI ACCGGT 2 cut(s) 445, 520
Asp718I GGTACC 2 cut(s) 442, 523
AspLEI GCGC 2 cut(s) 85, 283
AspS9I GGNCC 4 cut(s) 124, 286, 640, 650
AsuC2I CCSGG 6 cut(s) 43, 44, 63, 137, 647, 648
AsuHPI GGTGA 2 cut(s) 157, 649
AsuNHI GCTAGC 1 cut(s) 355
AvaI CYCGRG 3 cut(s) 42, 288, 646
AvaII GGWCC 2 cut(s) 124, 640
BaeGI GKGCMC 1 cut(s) 49
BaeI ACNNNNGTAYC 4 cut(s) 138, 171, 507, 540
BamHI GGATCC 1 cut(s) 116
BanI GGYRCC 5 cut(s) 82, 306, 406, 442, 523
BanII GRGCYC 1 cut(s) 625
Bbv12I GWGCWC 2 cut(s) 295, 625
BbvI GCAGC 1 cut(s) 358
BcgI CGANNNNNNTGC 2 cut(s) 675, 709
BciT130I CCWGG 3 cut(s) 338, 440, 616
BcnI CCSGG 6 cut(s) 43, 44, 63, 137, 647, 648
BcoDI GTCTC 4 cut(s) 116, 381, 603, 723
BfaI CTAG 3 cut(s) 296, 342, 356
BfmI CTRYAG 1 cut(s) 578
BfoI RGCGCY 2 cut(s) 86, 284
BglI GCCNNNNNGGC 1 cut(s) 581
BisI GCNGC 1 cut(s) 372
BlsI GCNGC 1 cut(s) 373
Bme1390I CCNGG 9 cut(s) 43, 44, 63, 137, 338, 440, 616, 647, 648
Bme18I GGWCC 2 cut(s) 124, 640
BmeT110I CYCGRG 3 cut(s) 42, 288, 646
BmgT120I GGNCC 4 cut(s) 124, 286, 640, 650
BmiI GGNNCC 8 cut(s) 84, 118, 126, 308, 408, 444, 525, 651
BmrFI CCNGG 9 cut(s) 43, 44, 63, 137, 338, 440, 616, 647, 648
BmsI GCATC 1 cut(s) 484
BmtI GCTAGC 1 cut(s) 359
BpmI CTGGAG 1 cut(s) 637
BpuEI CTTGAG 2 cut(s) 315, 490
BpuMI CCSGG 6 cut(s) 43, 44, 63, 137, 647, 648
BsaHI GRCGYC 1 cut(s) 83
BsaI GGTCTC 2 cut(s) 116, 723
BsaJI CCNNGG 6 cut(s) 42, 106, 120, 336, 646, 647
BsaWI WCCGGW 3 cut(s) 151, 445, 520
Bsc4I CCNNNNNNNGG 4 cut(s) 142, 383, 419, 445
Bse118I RCCGGY 3 cut(s) 403, 445, 520
Bse1I ACTGG 1 cut(s) 503
BseBI CCWGG 3 cut(s) 338, 440, 616
BseDI CCNNGG 6 cut(s) 42, 106, 120, 336, 646, 647
BseGI GGATG 1 cut(s) 347
BseLI CCNNNNNNNGG 4 cut(s) 142, 383, 419, 445
BseMII CTCAG 1 cut(s) 171
BseNI ACTGG 1 cut(s) 503
BseSI GKGCMC 1 cut(s) 49
BseXI GCAGC 1 cut(s) 358
BseYI CCCAGC 1 cut(s) 351
BshFI GGCC 4 cut(s) 237, 287, 596, 652
BshNI GGYRCC 5 cut(s) 82, 306, 406, 442, 523
BshTI ACCGGT 2 cut(s) 445, 520
BsiHKAI GWGCWC 2 cut(s) 295, 625
BsiHKCI CYCGRG 3 cut(s) 42, 288, 646
BsiSI CCGG 8 cut(s) 43, 62, 137, 152, 404, 446, 521, 647
BslFI GGGAC 1 cut(s) 735
BslI CCNNNNNNNGG 4 cut(s) 142, 383, 419, 445
BsmAI GTCTC 4 cut(s) 116, 381, 603, 723
BsmFI GGGAC 1 cut(s) 735
BsmI GAATGC 1 cut(s) 258
BsnI GGCC 4 cut(s) 237, 287, 596, 652
Bso31I GGTCTC 2 cut(s) 116, 723
BsoBI CYCGRG 3 cut(s) 42, 288, 646
Bsp1286I GDGCHC 3 cut(s) 49, 295, 625
Bsp143I GATC 5 cut(s) 116, 139, 183, 199, 344
Bsp19I CCATGG 1 cut(s) 106
BspACI CCGC 2 cut(s) 399, 643
BspANI GGCC 4 cut(s) 237, 287, 596, 652
BspCNI CTCAG 1 cut(s) 172
BspLI GGNNCC 8 cut(s) 84, 118, 126, 308, 408, 444, 525, 651
BspMAI CTGCAG 1 cut(s) 582
BspOI GCTAGC 1 cut(s) 359
BspPI GGATC 4 cut(s) 111, 124, 147, 207
BspT107I GGYRCC 5 cut(s) 82, 306, 406, 442, 523
BspTNI GGTCTC 2 cut(s) 116, 723
BsrFI RCCGGY 3 cut(s) 403, 445, 520
BsrI ACTGG 1 cut(s) 503
BssAI RCCGGY 3 cut(s) 403, 445, 520
BssECI CCNNGG 6 cut(s) 42, 106, 120, 336, 646, 647
BssMI GATC 5 cut(s) 116, 139, 183, 199, 344
BssNI GRCGYC 1 cut(s) 83
BssT1I CCWWGG 2 cut(s) 106, 120
Bst2UI CCWGG 3 cut(s) 338, 440, 616
Bst4CI ACNGT 3 cut(s) 492, 512, 632
BstACI GRCGYC 1 cut(s) 83
BstC8I GCNNGC 6 cut(s) 87, 285, 357, 361, 582, 675
BstDEI CTNAG 1 cut(s) 180
BstDSI CCRYGG 1 cut(s) 106
BstF5I GGATG 1 cut(s) 347
BstH2I RGCGCY 2 cut(s) 86, 284
BstHHI GCGC 2 cut(s) 85, 283
BstKTI GATC 5 cut(s) 119, 142, 186, 202, 347
BstMAI GTCTC 4 cut(s) 116, 381, 603, 723
BstMBI GATC 5 cut(s) 116, 139, 183, 199, 344
BstMWI GCNNNNNNNGC 2 cut(s) 581, 683
BstNI CCWGG 3 cut(s) 338, 440, 616
BstNSI RCATGY 1 cut(s) 575
BstSCI CCNGG 9 cut(s) 41, 42, 61, 135, 336, 438, 614, 645, 646
BstSFI CTRYAG 1 cut(s) 578
BstSLI GKGCMC 1 cut(s) 49
BstV1I GCAGC 1 cut(s) 358
BstX2I RGATCY 1 cut(s) 116
BstYI RGATCY 1 cut(s) 116
BsuRI GGCC 4 cut(s) 237, 287, 596, 652
BtgI CCRYGG 1 cut(s) 106
BtsCI GGATG 1 cut(s) 347
BtsIMutI CAGTG 2 cut(s) 47, 701
Cac8I GCNNGC 6 cut(s) 87, 285, 357, 361, 582, 675
CfoI GCGC 2 cut(s) 85, 283
Cfr10I RCCGGY 3 cut(s) 403, 445, 520
Cfr13I GGNCC 4 cut(s) 124, 286, 640, 650
Cfr9I CCCGGG 2 cut(s) 42, 646
Csp6I GTAC 6 cut(s) 216, 323, 443, 524, 551, 590
CspAI ACCGGT 2 cut(s) 445, 520
CviAII CATG 4 cut(s) 107, 277, 364, 572
CviQI GTAC 6 cut(s) 216, 323, 443, 524, 551, 590
DdeI CTNAG 1 cut(s) 180
DinI GGCGCC 1 cut(s) 84
DpnI GATC 5 cut(s) 118, 141, 185, 201, 346
DpnII GATC 5 cut(s) 116, 139, 183, 199, 344
Ecl136II GAGCTC 1 cut(s) 623
Eco130I CCWWGG 2 cut(s) 106, 120
Eco24I GRGCYC 1 cut(s) 625
Eco31I GGTCTC 2 cut(s) 116, 723
Eco47I GGWCC 2 cut(s) 124, 640
Eco53kI GAGCTC 1 cut(s) 623
Eco57I CTGAAG 2 cut(s) 178, 725
Eco88I CYCGRG 3 cut(s) 42, 288, 646
EcoICRI GAGCTC 1 cut(s) 623
EcoO109I RGGNCCY 1 cut(s) 650
EcoRII CCWGG 3 cut(s) 336, 438, 614
EcoT14I CCWWGG 2 cut(s) 106, 120
EcoT38I GRGCYC 1 cut(s) 625
EgeI GGCGCC 1 cut(s) 84
EheI GGCGCC 1 cut(s) 84
ErhI CCWWGG 2 cut(s) 106, 120
FaeI CATG 4 cut(s) 110, 280, 367, 575
FaiI YATR 7 cut(s) 108, 173, 278, 365, 573, 717, 748
FaqI GGGAC 1 cut(s) 735
FatI CATG 4 cut(s) 106, 276, 363, 571
FblI GTMKAC 1 cut(s) 450
Fnu4HI GCNGC 1 cut(s) 372
FokI GGATG 1 cut(s) 334
FriOI GRGCYC 1 cut(s) 625
Fsp4HI GCNGC 1 cut(s) 372
FspBI CTAG 3 cut(s) 296, 342, 356
GlaI GCGC 2 cut(s) 84, 282
GluI GCNGC 1 cut(s) 372
GsaI CCCAGC 1 cut(s) 355
GsuI CTGGAG 1 cut(s) 637
HaeII RGCGCY 2 cut(s) 86, 284
HaeIII GGCC 4 cut(s) 237, 287, 596, 652
HapII CCGG 8 cut(s) 43, 62, 137, 152, 404, 446, 521, 647
HhaI GCGC 2 cut(s) 85, 283
Hin1I GRCGYC 1 cut(s) 83
Hin1II CATG 4 cut(s) 110, 280, 367, 575
Hin6I GCGC 2 cut(s) 83, 281
HinP1I GCGC 2 cut(s) 83, 281
HindIII AAGCTT 1 cut(s) 466
HinfI GANTC 2 cut(s) 148, 167
HpaII CCGG 8 cut(s) 43, 62, 137, 152, 404, 446, 521, 647
HphI GGTGA 2 cut(s) 157, 649
Hpy166II GTNNAC 3 cut(s) 451, 508, 535
Hpy188I TCNGA 4 cut(s) 116, 160, 429, 627
Hpy188III TCNNGA 1 cut(s) 203
Hpy8I GTNNAC 3 cut(s) 451, 508, 535
Hpy99I CGWCG 1 cut(s) 533
HpyAV CCTTC 1 cut(s) 202
HpyCH4III ACNGT 3 cut(s) 492, 512, 632
HpyCH4IV ACGT 1 cut(s) 531
HpyCH4V TGCA 2 cut(s) 256, 580
HpyF10VI GCNNNNNNNGC 2 cut(s) 581, 683
HpyF3I CTNAG 1 cut(s) 180
HpySE526I ACGT 1 cut(s) 531
Hsp92I GRCGYC 1 cut(s) 83
Hsp92II CATG 4 cut(s) 110, 280, 367, 575
HspAI GCGC 2 cut(s) 83, 281
KasI GGCGCC 1 cut(s) 82
KpnI GGTACC 2 cut(s) 446, 527
Kzo9I GATC 5 cut(s) 116, 139, 183, 199, 344
Lsp1109I GCAGC 1 cut(s) 358
LweI GCATC 1 cut(s) 484
MaeI CTAG 3 cut(s) 296, 342, 356
MaeII ACGT 1 cut(s) 531
MaeIII GTNAC 4 cut(s) 16, 145, 492, 632
MalI GATC 5 cut(s) 118, 141, 185, 201, 346
MboI GATC 5 cut(s) 116, 139, 183, 199, 344
MboII GAAGA 2 cut(s) 278, 718
MflI RGATCY 1 cut(s) 116
MhlI GDGCHC 3 cut(s) 49, 295, 625
MluCI AATT 1 cut(s) 161
Mly113I GGCGCC 1 cut(s) 83
MlyI GAGTC 2 cut(s) 142, 176
MseI TTAA 1 cut(s) 434
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 8 cut(s) 43, 62, 137, 152, 404, 446, 521, 647
MspR9I CCNGG 9 cut(s) 43, 44, 63, 137, 338, 440, 616, 647, 648
Mva1269I GAATGC 1 cut(s) 258
MvaI CCWGG 3 cut(s) 338, 440, 616
MwoI GCNNNNNNNGC 2 cut(s) 581, 683
NarI GGCGCC 1 cut(s) 83
NciI CCSGG 6 cut(s) 43, 44, 63, 137, 647, 648
NcoI CCATGG 1 cut(s) 106
NdeII GATC 5 cut(s) 116, 139, 183, 199, 344
NheI GCTAGC 1 cut(s) 355
NlaIII CATG 4 cut(s) 110, 280, 367, 575
NlaIV GGNNCC 8 cut(s) 84, 118, 126, 308, 408, 444, 525, 651
NmuCI GTSAC 2 cut(s) 16, 145
NspI RCATGY 1 cut(s) 575
PctI GAATGC 1 cut(s) 258
PfoI TCCNGGA 2 cut(s) 61, 135
PinAI ACCGGT 2 cut(s) 445, 520
PkrI GCNGC 1 cut(s) 373
PleI GAGTC 2 cut(s) 142, 175
PluTI GGCGCC 1 cut(s) 86
PpsI GAGTC 2 cut(s) 142, 175
Psp124BI GAGCTC 1 cut(s) 625
Psp6I CCWGG 3 cut(s) 336, 438, 614
PspFI CCCAGC 1 cut(s) 351
PspGI CCWGG 3 cut(s) 336, 438, 614
PspN4I GGNNCC 8 cut(s) 84, 118, 126, 308, 408, 444, 525, 651
PspPI GGNCC 4 cut(s) 124, 286, 640, 650
PstI CTGCAG 1 cut(s) 582
PsuI RGATCY 1 cut(s) 116
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 6 cut(s) 217, 324, 444, 525, 552, 591
RsaNI GTAC 6 cut(s) 216, 323, 443, 524, 551, 590
SacI GAGCTC 1 cut(s) 625
SaqAI TTAA 1 cut(s) 434
SatI GCNGC 1 cut(s) 372
Sau3AI GATC 5 cut(s) 116, 139, 183, 199, 344
Sau96I GGNCC 4 cut(s) 124, 286, 640, 650
SbfI CCTGCAGG 1 cut(s) 582
SchI GAGTC 2 cut(s) 142, 176
ScrFI CCNGG 9 cut(s) 43, 44, 63, 137, 338, 440, 616, 647, 648
SdaI CCTGCAGG 1 cut(s) 582
SduI GDGCHC 3 cut(s) 49, 295, 625
SfaNI GCATC 1 cut(s) 484
SfcI CTRYAG 1 cut(s) 578
SfoI GGCGCC 1 cut(s) 84
SinI GGWCC 2 cut(s) 124, 640
SmaI CCCGGG 2 cut(s) 44, 648
SmlI CTYRAG 2 cut(s) 330, 469
SmoI CTYRAG 2 cut(s) 330, 469
SrfI GCCCGGGC 1 cut(s) 44
Sse8387I CCTGCAGG 1 cut(s) 582
Sse9I AATT 1 cut(s) 161
SsiI CCGC 2 cut(s) 399, 643
SspDI GGCGCC 1 cut(s) 82
SspMI CTAG 3 cut(s) 296, 342, 356
SstI GAGCTC 1 cut(s) 625
StyD4I CCNGG 9 cut(s) 41, 42, 61, 135, 336, 438, 614, 645, 646
StyI CCWWGG 2 cut(s) 106, 120
TaaI ACNGT 3 cut(s) 492, 512, 632
TaiI ACGT 1 cut(s) 534
TaqI TCGA 2 cut(s) 682, 695
TasI AATT 1 cut(s) 161
Tru1I TTAA 1 cut(s) 434
Tru9I TTAA 1 cut(s) 434
TscAI CASTG 2 cut(s) 54, 708
TseFI GTSAC 2 cut(s) 16, 145
TseI GCWGC 1 cut(s) 371
Tsp45I GTSAC 2 cut(s) 16, 145
TspDTI ATGAA 3 cut(s) 17, 285, 380
TspGWI ACGGA 2 cut(s) 226, 431
TspMI CCCGGG 2 cut(s) 42, 646
TspRI CASTG 2 cut(s) 54, 708
VpaK11BI GGWCC 2 cut(s) 124, 640
XceI RCATGY 1 cut(s) 575
XmaI CCCGGG 2 cut(s) 42, 646
XmiI GTMKAC 1 cut(s) 450
XspI CTAG 3 cut(s) 296, 342, 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.