Rroxscaffold_2G00101670

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000633
Physical Location & Seq
Forward (+)
23566224 .. 23567697
1474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00101670.1

Sequence Viewer

Length: 627 bp
ATGGCCGGGTTCACGACCCACCAGGGCGGAGCTCATATGGGATGTCACCCCGGACACCCGGGGCCTGGGGTATGGCTTGCACAGCCCATCAGCTTACACAAGAGAGGGCTGATTAGGCATCGGGAGAACAGACTTGCCTCCCACATCGAAGATAGGGGAAACTCATTTCCCATGCTAGGGAGTTGGATCGGTACCCAGGGCGGTATAGCATTACGTGAGGTATTGGAGAAAGCCACCAAAAACATTGGCGCGCCGGATAGGGGAGAATCATGGGCGAGCGGAAGAAACGACGGTGAGGGTAGAAGTGTCGCCGGGCACCGATATTCTCTCCGGGTCGGAGGGACCATTCGAACAAACATATCAAAGCCACCGAAGATTGACTATACGGGAGTAGGGGACCCGTGCGGCACCTACGAGGGCTTTCGCTCACAAACTAGTGCCAAAGGGTACACGGATGAGGAGACGCTTGTCGGTGAAGCTTTGAGTTGGTTCTACAAATTGCCGGCCGGTTACGTAGGGAATTTCAAGGAGCTCGCGGACAAGTTCGTAGCTCGATTCATCTTACGTCGATGGGATGCATACCCCGAAAGGCTGCTAAAGATTGAACGGGGGAAAATGAAACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

208

Amino Acids

22.93

Weight (kDa)

9.69

Isoelectric Point (pI)

37.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000633)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22376 FvH4_4g15485
rosa_laevigata RLG00000001772
rosa_multiflora Rmu_co7980664.1_g000001 Rmu_co7991850.1_g000001 Rmu_co8069086.1_g000001 Rmu_co8203420.1_g000001 Rmu_co8213206.1_g000001 Rmu_co8240317.1_g000001 Rmu_co8382323.1_g000001 Rmu_co8439419.1_g000001 Rmu_co8463919.1_g000001 Rmu_co8496399.1_g000001 Rmu_co8505885.1_g000001 Rmu_sc0000194.1_g000007 Rmu_sc0000194.1_g000008 Rmu_sc0000279.1_g000005 Rmu_sc0000367.1_g000177 Rmu_sc0000385.1_g000009 Rmu_sc0000423.1_g000016 Rmu_sc0000539.1_g000053 Rmu_sc0000779.1_g000017 Rmu_sc0000940.1_g000038 Rmu_sc0000942.1_g000025 Rmu_sc0001436.1_g000019 Rmu_sc0001477.1_g000004 Rmu_sc0001483.1_g000028 Rmu_sc0001518.1_g000005 Rmu_sc0001565.1_g000021 Rmu_sc0001655.1_g000010 Rmu_sc0001773.1_g000009 Rmu_sc0001782.1_g000037 Rmu_sc0001866.1_g000009 Rmu_sc0002054.1_g000022 Rmu_sc0002075.1_g000001 Rmu_sc0002264.1_g000016 Rmu_sc0002868.1_g000020 Rmu_sc0003033.1_g000062 Rmu_sc0003276.1_g000007 Rmu_sc0003486.1_g000003 Rmu_sc0003760.1_g000026 Rmu_sc0004391.1_g000020 Rmu_sc0004549.1_g000021 Rmu_sc0004835.1_g000002 Rmu_sc0005137.1_g000028 Rmu_sc0005178.1_g000022 Rmu_sc0005406.1_g000004 Rmu_sc0006059.1_g000064 Rmu_sc0006822.1_g000011 Rmu_sc0006997.1_g000014 Rmu_sc0007144.1_g000004 Rmu_sc0007321.1_g000001 Rmu_sc0007677.1_g000001 Rmu_sc0009120.1_g000003 Rmu_sc0009255.1_g000002 Rmu_sc0009427.1_g000001 Rmu_sc0009806.1_g000012 Rmu_sc0010048.1_g000010 Rmu_sc0011416.1_g000001 Rmu_sc0014844.1_g000011 Rmu_sc0018644.1_g000003 Rmu_sc0019345.1_g000006 Rmu_sc0025211.1_g000005 Rmu_sc0029888.1_g000001 Rmu_sc0035796.1_g000001 Rmu_ssc0000381.1_g000017
rosa_roxburghii Rroxscaffold_1G00018250 Rroxscaffold_2G00093100 Rroxscaffold_2G00101670 Rroxscaffold_3G00249740 Rroxscaffold_4G00310550 Rroxscaffold_4G00331340 Rroxscaffold_5G00337050 Rroxscaffold_5G00378590 Rroxscaffold_5G00378600 Rroxscaffold_6G00389000 Rroxscaffold_6G00401050
rosa_rugosa Rorug02G0247700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 191
AccB1I GGYRCC 3 cut(s) 191, 315, 407
AccBSI CCGCTC 1 cut(s) 279
AccII CGCG 2 cut(s) 251, 536
AciI CCGC 5 cut(s) 27, 201, 279, 405, 536
AclWI GGATC 1 cut(s) 194
AcoI YGGCCR 2 cut(s) 3, 504
AcsI RAATTY 1 cut(s) 520
AfaI GTAC 2 cut(s) 193, 449
AfiI CCNNNNNNNGG 4 cut(s) 65, 176, 177, 260
AgsI TTSAA 2 cut(s) 526, 605
AhlI ACTAGT 1 cut(s) 434
AjnI CCWGG 3 cut(s) 21, 64, 195
AluBI AGCT 5 cut(s) 32, 93, 479, 532, 551
AluI AGCT 5 cut(s) 32, 93, 479, 532, 551
Alw21I GWGCWC 2 cut(s) 34, 534
Alw26I GTCTC 1 cut(s) 455
AlwI GGATC 1 cut(s) 194
Ama87I CYCGRG 1 cut(s) 58
AoxI GGCC 3 cut(s) 3, 62, 504
ApeKI GCWGC 1 cut(s) 592
ApoI RAATTY 1 cut(s) 520
AscI GGCGCGCC 1 cut(s) 249
Asp718I GGTACC 1 cut(s) 191
AspLEI GCGC 2 cut(s) 251, 253
AspS9I GGNCC 3 cut(s) 62, 342, 397
AsuC2I CCSGG 6 cut(s) 7, 51, 59, 60, 313, 332
AsuHPI GGTGA 3 cut(s) 38, 305, 485
AsuII TTCGAA 1 cut(s) 349
AvaI CYCGRG 1 cut(s) 58
AvaII GGWCC 2 cut(s) 342, 397
BaeGI GKGCMC 1 cut(s) 318
BanI GGYRCC 3 cut(s) 191, 315, 407
BanII GRGCYC 2 cut(s) 34, 534
Bbv12I GWGCWC 2 cut(s) 34, 534
BbvI GCAGC 1 cut(s) 579
BccI CCATC 2 cut(s) 95, 564
BciT130I CCWGG 3 cut(s) 23, 66, 197
BcnI CCSGG 6 cut(s) 7, 51, 59, 60, 313, 332
BcoDI GTCTC 1 cut(s) 455
BcuI ACTAGT 1 cut(s) 434
BfaI CTAG 2 cut(s) 176, 435
BisI GCNGC 2 cut(s) 406, 593
BlsI GCNGC 2 cut(s) 407, 594
Bme1390I CCNGG 9 cut(s) 7, 23, 51, 59, 60, 66, 197, 313, 332
Bme18I GGWCC 2 cut(s) 342, 397
BmeT110I CYCGRG 1 cut(s) 58
BmgT120I GGNCC 3 cut(s) 62, 342, 397
BmiI GGNNCC 7 cut(s) 63, 193, 317, 343, 398, 399, 409
BmrFI CCNGG 9 cut(s) 7, 23, 51, 59, 60, 66, 197, 313, 332
BmsI GCATC 2 cut(s) 127, 565
BoxI GACNNNNGTC 1 cut(s) 467
Bpu14I TTCGAA 1 cut(s) 349
BpuMI CCSGG 6 cut(s) 7, 51, 59, 60, 313, 332
BsaAI YACGTR 2 cut(s) 215, 514
BsaJI CCNNGG 7 cut(s) 22, 49, 58, 59, 65, 195, 196
Bsc4I CCNNNNNNNGG 4 cut(s) 65, 176, 177, 260
Bse118I RCCGGY 2 cut(s) 502, 506
BseBI CCWGG 3 cut(s) 23, 66, 197
BseDI CCNNGG 7 cut(s) 22, 49, 58, 59, 65, 195, 196
BseGI GGATG 3 cut(s) 47, 460, 580
BseLI CCNNNNNNNGG 4 cut(s) 65, 176, 177, 260
BsePI GCGCGC 1 cut(s) 249
BseRI GAGGAG 1 cut(s) 473
BseSI GKGCMC 1 cut(s) 318
BseX3I CGGCCG 1 cut(s) 504
BseXI GCAGC 1 cut(s) 579
Bsh1236I CGCG 2 cut(s) 251, 536
Bsh1285I CGRYCG 1 cut(s) 507
BshFI GGCC 3 cut(s) 5, 64, 506
BshNI GGYRCC 3 cut(s) 191, 315, 407
BsiEI CGRYCG 1 cut(s) 507
BsiHKAI GWGCWC 2 cut(s) 34, 534
BsiHKCI CYCGRG 1 cut(s) 58
BsiSI CCGG 8 cut(s) 6, 51, 59, 254, 312, 331, 503, 507
BslFI GGGAC 2 cut(s) 355, 410
BslI CCNNNNNNNGG 4 cut(s) 65, 176, 177, 260
BsmAI GTCTC 1 cut(s) 455
BsmBI CGTCTC 1 cut(s) 455
BsmFI GGGAC 2 cut(s) 355, 410
BsnI GGCC 3 cut(s) 5, 64, 506
BsoBI CYCGRG 1 cut(s) 58
Bsp119I TTCGAA 1 cut(s) 349
Bsp1286I GDGCHC 3 cut(s) 34, 318, 534
Bsp143I GATC 1 cut(s) 186
BspACI CCGC 5 cut(s) 27, 201, 279, 405, 536
BspANI GGCC 3 cut(s) 5, 64, 506
BspFNI CGCG 2 cut(s) 251, 536
BspLI GGNNCC 7 cut(s) 63, 193, 317, 343, 398, 399, 409
BspPI GGATC 1 cut(s) 194
BspT104I TTCGAA 1 cut(s) 349
BspT107I GGYRCC 3 cut(s) 191, 315, 407
BsrBI CCGCTC 1 cut(s) 279
BsrFI RCCGGY 2 cut(s) 502, 506
BssAI RCCGGY 2 cut(s) 502, 506
BssECI CCNNGG 7 cut(s) 22, 49, 58, 59, 65, 195, 196
BssHII GCGCGC 1 cut(s) 249
BssMI GATC 1 cut(s) 186
Bst2UI CCWGG 3 cut(s) 23, 66, 197
Bst4CI ACNGT 1 cut(s) 293
BstBAI YACGTR 2 cut(s) 215, 514
BstBI TTCGAA 1 cut(s) 349
BstC8I GCNNGC 5 cut(s) 78, 251, 277, 504, 534
BstF5I GGATG 3 cut(s) 47, 460, 580
BstFNI CGCG 2 cut(s) 251, 536
BstHHI GCGC 2 cut(s) 251, 253
BstKTI GATC 1 cut(s) 189
BstMAI GTCTC 1 cut(s) 455
BstMBI GATC 1 cut(s) 186
BstMCI CGRYCG 1 cut(s) 507
BstMWI GCNNNNNNNGC 2 cut(s) 82, 115
BstNI CCWGG 3 cut(s) 23, 66, 197
BstPAI GACNNNNGTC 1 cut(s) 467
BstSCI CCNGG 9 cut(s) 5, 21, 49, 57, 58, 64, 195, 311, 330
BstSLI GKGCMC 1 cut(s) 318
BstSNI TACGTA 1 cut(s) 514
BstUI CGCG 2 cut(s) 251, 536
BstV1I GCAGC 1 cut(s) 579
BstZI CGGCCG 1 cut(s) 504
BsuRI GGCC 3 cut(s) 5, 64, 506
BtsCI GGATG 3 cut(s) 47, 460, 580
Cac8I GCNNGC 5 cut(s) 78, 251, 277, 504, 534
CfoI GCGC 2 cut(s) 251, 253
Cfr10I RCCGGY 2 cut(s) 502, 506
Cfr13I GGNCC 3 cut(s) 62, 342, 397
Cfr9I CCCGGG 1 cut(s) 58
CseI GACGC 1 cut(s) 472
Csp6I GTAC 2 cut(s) 192, 448
CviAII CATG 2 cut(s) 172, 270
CviQI GTAC 2 cut(s) 192, 448
DpnI GATC 1 cut(s) 188
DpnII GATC 1 cut(s) 186
EaeI YGGCCR 2 cut(s) 3, 504
EagI CGGCCG 1 cut(s) 504
EciI GGCGGA 1 cut(s) 42
Ecl136II GAGCTC 2 cut(s) 32, 532
EclXI CGGCCG 1 cut(s) 504
Eco105I TACGTA 1 cut(s) 514
Eco24I GRGCYC 2 cut(s) 34, 534
Eco47I GGWCC 2 cut(s) 342, 397
Eco52I CGGCCG 1 cut(s) 504
Eco53kI GAGCTC 2 cut(s) 32, 532
Eco88I CYCGRG 1 cut(s) 58
EcoICRI GAGCTC 2 cut(s) 32, 532
EcoO109I RGGNCCY 2 cut(s) 62, 397
EcoRII CCWGG 3 cut(s) 21, 64, 195
EcoT22I ATGCAT 1 cut(s) 580
EcoT38I GRGCYC 2 cut(s) 34, 534
Esp3I CGTCTC 1 cut(s) 455
FaeI CATG 2 cut(s) 175, 273
FaiI YATR 9 cut(s) 36, 38, 73, 173, 206, 271, 359, 384, 580
FaqI GGGAC 2 cut(s) 355, 410
FatI CATG 2 cut(s) 171, 269
FauNDI CATATG 1 cut(s) 36
Fnu4HI GCNGC 2 cut(s) 406, 593
FokI GGATG 3 cut(s) 54, 467, 587
FriOI GRGCYC 2 cut(s) 34, 534
Fsp4HI GCNGC 2 cut(s) 406, 593
FspBI CTAG 2 cut(s) 176, 435
GlaI GCGC 2 cut(s) 250, 252
GluI GCNGC 2 cut(s) 406, 593
HaeIII GGCC 3 cut(s) 5, 64, 506
HapII CCGG 8 cut(s) 6, 51, 59, 254, 312, 331, 503, 507
HgaI GACGC 1 cut(s) 472
HhaI GCGC 2 cut(s) 251, 253
Hin1II CATG 2 cut(s) 175, 273
Hin6I GCGC 2 cut(s) 249, 251
HinP1I GCGC 2 cut(s) 249, 251
HindIII AAGCTT 1 cut(s) 477
HinfI GANTC 2 cut(s) 266, 555
HpaII CCGG 8 cut(s) 6, 51, 59, 254, 312, 331, 503, 507
HphI GGTGA 3 cut(s) 38, 305, 485
Hpy166II GTNNAC 2 cut(s) 12, 450
Hpy188I TCNGA 1 cut(s) 338
Hpy188III TCNNGA 2 cut(s) 13, 122
Hpy8I GTNNAC 2 cut(s) 12, 450
Hpy99I CGWCG 2 cut(s) 293, 570
HpyCH4III ACNGT 1 cut(s) 293
HpyCH4IV ACGT 3 cut(s) 214, 513, 565
HpyCH4V TGCA 2 cut(s) 80, 578
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 115
HpySE526I ACGT 3 cut(s) 214, 513, 565
Hsp92II CATG 2 cut(s) 175, 273
HspAI GCGC 2 cut(s) 249, 251
KflI GGGWCCC 1 cut(s) 397
KpnI GGTACC 1 cut(s) 195
KroI GCCGGC 1 cut(s) 502
KroNI GCCGGC 1 cut(s) 504
Kzo9I GATC 1 cut(s) 186
LmnI GCTCC 2 cut(s) 29, 529
Lsp1109I GCAGC 1 cut(s) 579
LweI GCATC 2 cut(s) 127, 565
MaeI CTAG 2 cut(s) 176, 435
MaeII ACGT 3 cut(s) 214, 513, 565
MaeIII GTNAC 2 cut(s) 44, 509
MalI GATC 1 cut(s) 188
MbiI CCGCTC 1 cut(s) 279
MboI GATC 1 cut(s) 186
MboII GAAGA 3 cut(s) 161, 294, 385
MhlI GDGCHC 3 cut(s) 34, 318, 534
MluCI AATT 2 cut(s) 497, 520
MmeI TCCRAC 2 cut(s) 164, 316
MnlI CCTC 7 cut(s) 98, 148, 211, 289, 332, 409, 451
Mph1103I ATGCAT 1 cut(s) 580
MroNI GCCGGC 1 cut(s) 502
MspI CCGG 8 cut(s) 6, 51, 59, 254, 312, 331, 503, 507
MspR9I CCNGG 9 cut(s) 7, 23, 51, 59, 60, 66, 197, 313, 332
MvaI CCWGG 3 cut(s) 23, 66, 197
MvnI CGCG 2 cut(s) 251, 536
MwoI GCNNNNNNNGC 2 cut(s) 82, 115
NaeI GCCGGC 1 cut(s) 504
NciI CCSGG 6 cut(s) 7, 51, 59, 60, 313, 332
NdeI CATATG 1 cut(s) 36
NdeII GATC 1 cut(s) 186
NgoMIV GCCGGC 1 cut(s) 502
NlaIII CATG 2 cut(s) 175, 273
NlaIV GGNNCC 7 cut(s) 63, 193, 317, 343, 398, 399, 409
NmuCI GTSAC 1 cut(s) 44
NsiI ATGCAT 1 cut(s) 580
NspV TTCGAA 1 cut(s) 349
PalAI GGCGCGCC 1 cut(s) 249
PasI CCCWGGG 1 cut(s) 196
PauI GCGCGC 1 cut(s) 249
PdiI GCCGGC 1 cut(s) 504
PfeI GAWTC 2 cut(s) 266, 555
PkrI GCNGC 2 cut(s) 407, 594
Ppu21I YACGTR 2 cut(s) 215, 514
PpuMI RGGWCCY 1 cut(s) 397
PshAI GACNNNNGTC 1 cut(s) 467
Psp124BI GAGCTC 2 cut(s) 34, 534
Psp5II RGGWCCY 1 cut(s) 397
Psp6I CCWGG 3 cut(s) 21, 64, 195
PspGI CCWGG 3 cut(s) 21, 64, 195
PspN4I GGNNCC 7 cut(s) 63, 193, 317, 343, 398, 399, 409
PspPI GGNCC 3 cut(s) 62, 342, 397
PspPPI RGGWCCY 1 cut(s) 397
PteI GCGCGC 1 cut(s) 249
RsaI GTAC 2 cut(s) 193, 449
RsaNI GTAC 2 cut(s) 192, 448
SacI GAGCTC 2 cut(s) 34, 534
SatI GCNGC 2 cut(s) 406, 593
Sau3AI GATC 1 cut(s) 186
Sau96I GGNCC 3 cut(s) 62, 342, 397
ScrFI CCNGG 9 cut(s) 7, 23, 51, 59, 60, 66, 197, 313, 332
SduI GDGCHC 3 cut(s) 34, 318, 534
SfaNI GCATC 2 cut(s) 127, 565
SfuI TTCGAA 1 cut(s) 349
SgsI GGCGCGCC 1 cut(s) 249
SinI GGWCC 2 cut(s) 342, 397
SmaI CCCGGG 1 cut(s) 60
SnaBI TACGTA 1 cut(s) 514
SpeI ACTAGT 1 cut(s) 434
Sse9I AATT 2 cut(s) 497, 520
SsiI CCGC 5 cut(s) 27, 201, 279, 405, 536
SspMI CTAG 2 cut(s) 176, 435
SstI GAGCTC 2 cut(s) 34, 534
StyD4I CCNGG 9 cut(s) 5, 21, 49, 57, 58, 64, 195, 311, 330
TaaI ACNGT 1 cut(s) 293
TaiI ACGT 3 cut(s) 217, 516, 568
TaqI TCGA 4 cut(s) 147, 349, 553, 568
TasI AATT 2 cut(s) 497, 520
TauI GCSGC 1 cut(s) 408
TfiI GAWTC 2 cut(s) 266, 555
TseFI GTSAC 1 cut(s) 44
TseI GCWGC 1 cut(s) 592
Tsp45I GTSAC 1 cut(s) 44
TspDTI ATGAA 1 cut(s) 547
TspGWI ACGGA 1 cut(s) 467
TspMI CCCGGG 1 cut(s) 58
VpaK11BI GGWCC 2 cut(s) 342, 397
XapI RAATTY 1 cut(s) 520
XmaI CCCGGG 1 cut(s) 58
XspI CTAG 2 cut(s) 176, 435
Zsp2I ATGCAT 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.